Bhi06G000138 (gene) Wax gourd

NameBhi06G000138
Typegene
OrganismBenincasa hispida (Wax gourd)
DescriptionEndo-1,3(4)-beta-glucanase
Locationchr6 : 3393285 .. 3395798 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCACTGTTCCCTAGGGAATAATGACGTTACACTTTTCCCTTTTTAAAAAATACAAATTAGAACGTTAAGAACTCATCATTTCTTTCTTATTTTAATACATCATTTTCATTTATGACCTTCCACAATCCTCCCTTTTATAATTATAAAATCCTCTCCACCAATAATCCCTCACTAGCCATGGAAACGCCTAAGCCATGGCTAATTTCCATCTTCTTCTTCTTCTTCATTATTCTCACTGCATTTCCGCCGGCTGTTTCCGGCCAATTTCTCTTCCCCCAAACCACCTCCACGGCGGTTCCCGACCCCGCCAAATTCTTCTCTCCCACTCTCCTCTCCTCTCCCCTTCCCACTAATTCCTTCTTCCAGAACTTTGTCCTCAACAATGGCGATAAGCCGGAATATATTCATCCGTATATCGTCAGAACGGCAAATTCTTCGCTCTCTGTTTCATACCCATCTCGTATTTCCAATTCTTCAATCACCCAGTTGCAGTTCTACCCCGATCTCACCATTTCTGCACTCGACAGAACTTCCCCCGTTTCTAACACAACCCATTTCATCTCGTCCTTCAGCGATCTCGGTGTTGATTTGGACATTGGCGATTTCAGATTCCATCTTGTTCGTGGAAGTCCTTATTTGACTTTCTCTGTTCTGAAAATCTCCTCTGTTTCGATCTCAACCGGCAATGGCGTCCGGTCTGTTGATTCTTATGACGATTCTACAAAACATATCATTCGGTTGAACAATGGACGGAATTGGGTACTTTATTCCTCGTCGGCGATTTATCTGATTAAATCGAAGAATAATCAGATTGTTACTTCCGGTGGATTTTCCGGCGTGATTCGGATTGCTGTTTTGCCAAATTCAGCGATGGAATCGGGGAACATTCTTGATCGGTACAGTGGATGTTACCCTGTTTCTGGGTATGTGAAGTTGTCTGAAAATTTTGGGTTCAAATACAAATGGCAGAAGAAAGGAAGTGGCGGCCTTCTCATGTTGGCTCACCCATTGCACCGGCAAATTTTGTCGAGAAATCAAACCGTTCTTCCGAATCTCCGGTACGGCAGCATCGACGGCGACCTTCTGGGAGTCGTCGGAGATTCGTGGGATTTGAATTTTAATCCGGTTCCGATCACTTGGCATTCAATCAACGGAATCGACAAAAAGTTCTTCCCTGAAATTGTCGCGGCTCTTAAACGAGACGTTGCGACTTTAAACGTTACAGAGCTTTCGTCAACGGCGTCGTCTTATTTCTACGCAAAGTTGTTGGCCAGAGCTGCGAGATTGGCACTGATCGCAGAGGAGGTGGATAACGGCGCCGGTGTCATTCCGGCGGTGGTGAGATTCCTGAAAAACGGGATTCAGCCATGGCTGATCGGGAAATTTCCGAAAAATGGATTTCTATATGAAAGAAAATGGGCTGGATTGGTAACGAAGAACGGAGCTACAAGTACAACAGAGGATTTCGGATTTGGAATCTACAACGATCATCATTTCCATTTGGGTTATTTCGTGTACTCAATCGCCGTTCTTGCCAAACTCGACCCAAATTGGGGAAAACAATTCAAGCCTCAAGCGTACGCTTTGGTTTACGATTACATGAATTTCAGACCCAAAAAATCCCAATTCTCAATTCCGTTTAGAAACTTCGATTTCTGGAAGCTCCACTCGTGGGCCGCCGGATTAACGGAATTCCCCGACGGCCGGAATCAGGAAAGCACAAGCGAAGCCGTCAACGCCTATTACGCGGCGGCGCTGATGGGTTTGGCTTACGACGATGAGTCTCTGACGGCCGCTGGGTCGACCCTGACTGCGGCTGAGATTGCGGCGACGCAGACATGGTGGCATGTGAAGAGCGAAAAAAACTGGATTTACGACAAGGGATTTGAAGAGGAAAACAGAGTAGTGGGGATATTGTGGAGTGCGGCAAGGGAGAGTCGGTTGTGGTTTGCGCCGGCGGAGTGGAGGGAGTGTAGAGTTGGGATACAGGTGTTGCCGGTATTGCCGGTGTCGGAGAGGGTGTTTTCCGACAAGGGGTTTGTGAAGGAGGTTGTGGAGTGGGTGTCACGGGCGTTGGAACGGGAAGACGCCGGCGAAGGATGGAAGGGATTTGCGTATGCTTTGGAAGGGATTTATGATAAGAAGAGTGCGATTGAGAAGGTAAAGAAGTTGAAGAAGCATGATGATGGGAATTCTCTCAGTAATCTTTTGTGGTGGATTTACAGTCGGCCGGAGGGACGAGGCTGAAGACGGTAACTTGAAAACAGGGGAACAGGGGATTTTGATTGCCTAGTTTTCCACGTGTTACACGTGTCAATCTTTTCTTTTTCGTATTCTTTTCGAATTTATTAATCAAATTTACATTAAAAAAAAAAACAAAGAGAAAATATATATTTGAATTTGGCCACTTACCTCTAACACTAGTGTCGTGTTTCACATTGTTGATTGCATCAATGGGTCATTTTTTTTAATCATTTCTATTAATTGGTCAGTGAATAATATCATCAATTTTAA

mRNA sequence

CCACTGTTCCCTAGGGAATAATGACGTTACACTTTTCCCTTTTTAAAAAATACAAATTAGAACGTTAAGAACTCATCATTTCTTTCTTATTTTAATACATCATTTTCATTTATGACCTTCCACAATCCTCCCTTTTATAATTATAAAATCCTCTCCACCAATAATCCCTCACTAGCCATGGAAACGCCTAAGCCATGGCTAATTTCCATCTTCTTCTTCTTCTTCATTATTCTCACTGCATTTCCGCCGGCTGTTTCCGGCCAATTTCTCTTCCCCCAAACCACCTCCACGGCGGTTCCCGACCCCGCCAAATTCTTCTCTCCCACTCTCCTCTCCTCTCCCCTTCCCACTAATTCCTTCTTCCAGAACTTTGTCCTCAACAATGGCGATAAGCCGGAATATATTCATCCGTATATCGTCAGAACGGCAAATTCTTCGCTCTCTGTTTCATACCCATCTCGTATTTCCAATTCTTCAATCACCCAGTTGCAGTTCTACCCCGATCTCACCATTTCTGCACTCGACAGAACTTCCCCCGTTTCTAACACAACCCATTTCATCTCGTCCTTCAGCGATCTCGGTGTTGATTTGGACATTGGCGATTTCAGATTCCATCTTGTTCGTGGAAGTCCTTATTTGACTTTCTCTGTTCTGAAAATCTCCTCTGTTTCGATCTCAACCGGCAATGGCGTCCGGTCTGTTGATTCTTATGACGATTCTACAAAACATATCATTCGGTTGAACAATGGACGGAATTGGGTACTTTATTCCTCGTCGGCGATTTATCTGATTAAATCGAAGAATAATCAGATTGTTACTTCCGGTGGATTTTCCGGCGTGATTCGGATTGCTGTTTTGCCAAATTCAGCGATGGAATCGGGGAACATTCTTGATCGGTACAGTGGATGTTACCCTGTTTCTGGGTATGTGAAGTTGTCTGAAAATTTTGGGTTCAAATACAAATGGCAGAAGAAAGGAAGTGGCGGCCTTCTCATGTTGGCTCACCCATTGCACCGGCAAATTTTGTCGAGAAATCAAACCGTTCTTCCGAATCTCCGGTACGGCAGCATCGACGGCGACCTTCTGGGAGTCGTCGGAGATTCGTGGGATTTGAATTTTAATCCGGTTCCGATCACTTGGCATTCAATCAACGGAATCGACAAAAAGTTCTTCCCTGAAATTGTCGCGGCTCTTAAACGAGACGTTGCGACTTTAAACGTTACAGAGCTTTCGTCAACGGCGTCGTCTTATTTCTACGCAAAGTTGTTGGCCAGAGCTGCGAGATTGGCACTGATCGCAGAGGAGGTGGATAACGGCGCCGGTGTCATTCCGGCGGTGGTGAGATTCCTGAAAAACGGGATTCAGCCATGGCTGATCGGGAAATTTCCGAAAAATGGATTTCTATATGAAAGAAAATGGGCTGGATTGGTAACGAAGAACGGAGCTACAAGTACAACAGAGGATTTCGGATTTGGAATCTACAACGATCATCATTTCCATTTGGGTTATTTCGTGTACTCAATCGCCGTTCTTGCCAAACTCGACCCAAATTGGGGAAAACAATTCAAGCCTCAAGCGTACGCTTTGGTTTACGATTACATGAATTTCAGACCCAAAAAATCCCAATTCTCAATTCCGTTTAGAAACTTCGATTTCTGGAAGCTCCACTCGTGGGCCGCCGGATTAACGGAATTCCCCGACGGCCGGAATCAGGAAAGCACAAGCGAAGCCGTCAACGCCTATTACGCGGCGGCGCTGATGGGTTTGGCTTACGACGATGAGTCTCTGACGGCCGCTGGGTCGACCCTGACTGCGGCTGAGATTGCGGCGACGCAGACATGGTGGCATGTGAAGAGCGAAAAAAACTGGATTTACGACAAGGGATTTGAAGAGGAAAACAGAGTAGTGGGGATATTGTGGAGTGCGGCAAGGGAGAGTCGGTTGTGGTTTGCGCCGGCGGAGTGGAGGGAGTGTAGAGTTGGGATACAGGTGTTGCCGGTATTGCCGGTGTCGGAGAGGGTGTTTTCCGACAAGGGGTTTGTGAAGGAGGTTGTGGAGTGGGTGTCACGGGCGTTGGAACGGGAAGACGCCGGCGAAGGATGGAAGGGATTTGCGTATGCTTTGGAAGGGATTTATGATAAGAAGAGTGCGATTGAGAAGGTAAAGAAGTTGAAGAAGCATGATGATGGGAATTCTCTCAGTAATCTTTTGTGGTGGATTTACAGTCGGCCGGAGGGACGAGGCTGAAGACGGTAACTTGAAAACAGGGGAACAGGGGATTTTGATTGCCTAGTTTTCCACGTGTTACACGTGTCAATCTTTTCTTTTTCGTATTCTTTTCGAATTTATTAATCAAATTTACATTAAAAAAAAAAACAAAGAGAAAATATATATTTGAATTTGGCCACTTACCTCTAACACTAGTGTCGTGTTTCACATTGTTGATTGCATCAATGGGTCATTTTTTTTAATCATTTCTATTAATTGGTCAGTGAATAATATCATCAATTTTAA

Coding sequence (CDS)

ATGACCTTCCACAATCCTCCCTTTTATAATTATAAAATCCTCTCCACCAATAATCCCTCACTAGCCATGGAAACGCCTAAGCCATGGCTAATTTCCATCTTCTTCTTCTTCTTCATTATTCTCACTGCATTTCCGCCGGCTGTTTCCGGCCAATTTCTCTTCCCCCAAACCACCTCCACGGCGGTTCCCGACCCCGCCAAATTCTTCTCTCCCACTCTCCTCTCCTCTCCCCTTCCCACTAATTCCTTCTTCCAGAACTTTGTCCTCAACAATGGCGATAAGCCGGAATATATTCATCCGTATATCGTCAGAACGGCAAATTCTTCGCTCTCTGTTTCATACCCATCTCGTATTTCCAATTCTTCAATCACCCAGTTGCAGTTCTACCCCGATCTCACCATTTCTGCACTCGACAGAACTTCCCCCGTTTCTAACACAACCCATTTCATCTCGTCCTTCAGCGATCTCGGTGTTGATTTGGACATTGGCGATTTCAGATTCCATCTTGTTCGTGGAAGTCCTTATTTGACTTTCTCTGTTCTGAAAATCTCCTCTGTTTCGATCTCAACCGGCAATGGCGTCCGGTCTGTTGATTCTTATGACGATTCTACAAAACATATCATTCGGTTGAACAATGGACGGAATTGGGTACTTTATTCCTCGTCGGCGATTTATCTGATTAAATCGAAGAATAATCAGATTGTTACTTCCGGTGGATTTTCCGGCGTGATTCGGATTGCTGTTTTGCCAAATTCAGCGATGGAATCGGGGAACATTCTTGATCGGTACAGTGGATGTTACCCTGTTTCTGGGTATGTGAAGTTGTCTGAAAATTTTGGGTTCAAATACAAATGGCAGAAGAAAGGAAGTGGCGGCCTTCTCATGTTGGCTCACCCATTGCACCGGCAAATTTTGTCGAGAAATCAAACCGTTCTTCCGAATCTCCGGTACGGCAGCATCGACGGCGACCTTCTGGGAGTCGTCGGAGATTCGTGGGATTTGAATTTTAATCCGGTTCCGATCACTTGGCATTCAATCAACGGAATCGACAAAAAGTTCTTCCCTGAAATTGTCGCGGCTCTTAAACGAGACGTTGCGACTTTAAACGTTACAGAGCTTTCGTCAACGGCGTCGTCTTATTTCTACGCAAAGTTGTTGGCCAGAGCTGCGAGATTGGCACTGATCGCAGAGGAGGTGGATAACGGCGCCGGTGTCATTCCGGCGGTGGTGAGATTCCTGAAAAACGGGATTCAGCCATGGCTGATCGGGAAATTTCCGAAAAATGGATTTCTATATGAAAGAAAATGGGCTGGATTGGTAACGAAGAACGGAGCTACAAGTACAACAGAGGATTTCGGATTTGGAATCTACAACGATCATCATTTCCATTTGGGTTATTTCGTGTACTCAATCGCCGTTCTTGCCAAACTCGACCCAAATTGGGGAAAACAATTCAAGCCTCAAGCGTACGCTTTGGTTTACGATTACATGAATTTCAGACCCAAAAAATCCCAATTCTCAATTCCGTTTAGAAACTTCGATTTCTGGAAGCTCCACTCGTGGGCCGCCGGATTAACGGAATTCCCCGACGGCCGGAATCAGGAAAGCACAAGCGAAGCCGTCAACGCCTATTACGCGGCGGCGCTGATGGGTTTGGCTTACGACGATGAGTCTCTGACGGCCGCTGGGTCGACCCTGACTGCGGCTGAGATTGCGGCGACGCAGACATGGTGGCATGTGAAGAGCGAAAAAAACTGGATTTACGACAAGGGATTTGAAGAGGAAAACAGAGTAGTGGGGATATTGTGGAGTGCGGCAAGGGAGAGTCGGTTGTGGTTTGCGCCGGCGGAGTGGAGGGAGTGTAGAGTTGGGATACAGGTGTTGCCGGTATTGCCGGTGTCGGAGAGGGTGTTTTCCGACAAGGGGTTTGTGAAGGAGGTTGTGGAGTGGGTGTCACGGGCGTTGGAACGGGAAGACGCCGGCGAAGGATGGAAGGGATTTGCGTATGCTTTGGAAGGGATTTATGATAAGAAGAGTGCGATTGAGAAGGTAAAGAAGTTGAAGAAGCATGATGATGGGAATTCTCTCAGTAATCTTTTGTGGTGGATTTACAGTCGGCCGGAGGGACGAGGCTGA

Protein sequence

MTFHNPPFYNYKILSTNNPSLAMETPKPWLISIFFFFFIILTAFPPAVSGQFLFPQTTSTAVPDPAKFFSPTLLSSPLPTNSFFQNFVLNNGDKPEYIHPYIVRTANSSLSVSYPSRISNSSITQLQFYPDLTISALDRTSPVSNTTHFISSFSDLGVDLDIGDFRFHLVRGSPYLTFSVLKISSVSISTGNGVRSVDSYDDSTKHIIRLNNGRNWVLYSSSAIYLIKSKNNQIVTSGGFSGVIRIAVLPNSAMESGNILDRYSGCYPVSGYVKLSENFGFKYKWQKKGSGGLLMLAHPLHRQILSRNQTVLPNLRYGSIDGDLLGVVGDSWDLNFNPVPITWHSINGIDKKFFPEIVAALKRDVATLNVTELSSTASSYFYAKLLARAARLALIAEEVDNGAGVIPAVVRFLKNGIQPWLIGKFPKNGFLYERKWAGLVTKNGATSTTEDFGFGIYNDHHFHLGYFVYSIAVLAKLDPNWGKQFKPQAYALVYDYMNFRPKKSQFSIPFRNFDFWKLHSWAAGLTEFPDGRNQESTSEAVNAYYAAALMGLAYDDESLTAAGSTLTAAEIAATQTWWHVKSEKNWIYDKGFEEENRVVGILWSAARESRLWFAPAEWRECRVGIQVLPVLPVSERVFSDKGFVKEVVEWVSRALEREDAGEGWKGFAYALEGIYDKKSAIEKVKKLKKHDDGNSLSNLLWWIYSRPEGRG
BLAST of Bhi06G000138 vs. Swiss-Prot
Match: sp|D4AZ24|ENG1_ARTBC (Probable endo-1,3(4)-beta-glucanase ARB_01444 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_01444 PE=1 SV=1)

HSP 1 Score: 172.9 bits (437), Expect = 1.2e-41
Identity = 198/731 (27.09%), Postives = 311/731 (42.54%), Query Frame = 0

Query: 15  STNNPSLAMETPKPWLISIFXXXXXXXTAFPPAVSGQFLFPQTTSTAVP--------DPA 74
           ST+NP     +P   + S         +A P  ++GQ +F       +P         P 
Sbjct: 162 STSNPGPTATSPTSGIPS-------QPSAPPATMAGQDIFQPIAKDPIPANIKSRDDHPV 221

Query: 75  KFFSPTLLSSPLPTNSFFQNFVLNNGDKPEYIHPYIVRTANSSLSV-SYPSRISNSSITQ 134
           K       + P+ TN F+ NF L N     + HPY +  A    +  S+   IS+   +Q
Sbjct: 222 KANHIENPTGPISTNKFYANFFLGNQTSTTFTHPYTMIWAKGDKNASSFGMAISHVEPSQ 281

Query: 135 --------------LQFY------PDLTISALDRTSPVSNTTHFISSFSDLGVDLDIG-- 194
                         +++Y        L +SA +     + +     +FS   +    G  
Sbjct: 282 RATGEPNNKLPGNPVRYYINPVGIKSLVLSASELKESTTMSVAKPQAFSAQAILRPTGGS 341

Query: 195 --DFRFHLVRGSPYLTFSVLKISSVSISTGNGVRSVDSYDDST----KHIIRLNNGRNWV 254
                F LV+G  ++T ++      +I +    R V+          K+ I L + +NW+
Sbjct: 342 SESITFPLVQGMGFIT-AIYNNLQPAIQSAVLFRKVEPAGSPQGGIFKYKITLEDDKNWL 401

Query: 255 LY---SSSAIYLIKSKNNQIVTS-GGFSGVIRIAVLPNSAMESGNILDRYSGCY----PV 314
           LY    + A   +K + N++++   GF GVI++A  P SA E   I D+ +G Y     +
Sbjct: 402 LYVIPENGADPKLKLEGNKLISGPTGFKGVIQVAKNP-SAEEGEGIYDKSAGSYATDIKI 461

Query: 315 SGYVKLSENFGFKYKWQKKGSGG-LLMLAHPLHRQIL-SRNQTVLPNLRYG-SIDGDLLG 374
           SG V       +K+ ++K G G  L+M A P H +      +    N++   +  G    
Sbjct: 462 SGSVGTDGTGTYKFSFEKAGKGAPLVMYALPHHVESFDDATKNTKKNMKLSTTTKGMATA 521

Query: 375 VVGDSWDLNFNPVPIT-----W---HSINGIDKKFFPEIVAALKRDVATLNVTELSSTAS 434
            VGDSW +    +P++     W    S      +     + A+  +  + ++   ++  S
Sbjct: 522 CVGDSWTMVEGNLPLSMDFAPWKPGSSSQVTLSEGAKNAIKAVAGNELSQDMELQTNLNS 581

Query: 435 SYFYAKLLARAARLALIAEEVDNGAGVIPAVVRFLKNGIQPWLIGKFPKNGFLYERKWAG 494
            YF  K L + A      +E+          +  LK   + ++  K  +   +Y+  W G
Sbjct: 582 MYFSGKGLNKFAGAIYTVQELVGDKAAASGPLNSLKESFKRFVDNK-QQIPLVYDNVWKG 641

Query: 495 LVTKN--GATSTTEDFGFGIYNDHHFHLGYFVYSIAVLAKLDPNWGKQFKPQAYALVYDY 554
           +V+        T  DFG  +YNDHHFH GYF+ + A+L KLDP W    K     LV D 
Sbjct: 642 VVSSGTYEKGDTGLDFGNTLYNDHHFHYGYFILTAAILGKLDPAWLDANKAYVNMLVRDS 701

Query: 555 MNFRPKKSQFSIPF-RNFDFWKLHSWAAGLTEFPDGRNQESTSEAVNAYYAAALMGLAYD 614
            N       F  PF R FD++  HSWA GL E  DG++QESTSE     YA  + G    
Sbjct: 702 GNSVDNDEHF--PFSRAFDWYHGHSWAKGLFESSDGKDQESTSEDTMYAYAIKMWGKTSG 761

Query: 615 DESLTAAGSTLTAAEIAATQTWWHVKSEKNWIYDKGFEEENRVVGILWSAARESRLWF-A 674
           D+S+ A G+ L    +A T   + +    N    K F   N+V GIL+    +   +F A
Sbjct: 762 DKSMEARGN-LMLGILARTLNNYFLMKNDNVNQPKNF-IGNKVTGILFENKIDHTTYFGA 821

Query: 675 PAEWRECRVGIQVLPVLPVSERVFSDKGFVKEVVEWVS-----RALEREDAGEGWKGFAY 681
             E+ +   GI +LP+LP S    S + FVKE  EW +      A   E    GWKG  Y
Sbjct: 822 NLEYIQ---GIHMLPLLPNSAYTRSAE-FVKE--EWEAMFASGAAAPAEKVTGGWKGVLY 872

BLAST of Bhi06G000138 vs. Swiss-Prot
Match: sp|Q09850|ENG2_SCHPO (Putative endo-1,3(4)-beta-glucanase 2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=eng2 PE=3 SV=1)

HSP 1 Score: 169.9 bits (429), Expect = 1.0e-40
Identity = 177/680 (26.03%), Postives = 291/680 (42.79%), Query Frame = 0

Query: 55  PQTTSTAVPDPAKFFSPTLLSSPLPTNSFFQNFVLNNGDKPEYIHPYIVRTANSSLSVSY 114
           P   S A P P    +  L   P+ TN F+ N  +     P + HPY V   N   S  Y
Sbjct: 14  PVFPSRAHPSPPLGLTSNLF--PIQTNKFYGNLYIGTRHNPSWSHPYSVTWLNG--SSYY 73

Query: 115 PSRISNSSITQLQFYPD-----------------LTISALDRTS----PVSNTTHFISSF 174
              IS+   +Q  F PD                 + ISA +  S     +  + HF    
Sbjct: 74  GLAISHIDDSQRVFGPDPESVPCQYYFNPAGLYSIIISAREFASGNLLSLDQSRHFSIQA 133

Query: 175 SDLGVDLDIGDFRFHLVRGSPYLTFSVLKISSVSIST--GNGVRSVDSYDDSTKHIIRLN 234
           +        G     +V G  +++     ++ V  S+   + +  + ++    K+ I+L 
Sbjct: 134 TLSATTSGSGTIILPIVAGMGFVSGYYTNLTPVFNSSILFSSITKI-NFSRGYKYRIQLT 193

Query: 235 NGRNWVLY-----SSSAIYLIKSKNNQIVTSGGFSGVIRIAVLPNSAMESG---NILDRY 294
           +G+ W LY     SSS   L  + N+Q+ TS  F+G+I+I  +PN ++ +     I D  
Sbjct: 194 DGKIWFLYAFPTSSSSTFNLTLASNSQLTTSTKFTGLIQICKVPNESVNNSYPDTIYDAS 253

Query: 295 SGCYPVSGYVKLSENFGFKYKWQKKGSGGL-----LMLAHPLHRQIL-SRNQTVLPNLRY 354
           +G Y  S  +    +      W +  + G      LM A P H Q   S  Q     L  
Sbjct: 254 AGVYTTSISLSAQVSGTTGEYWFRFATAGYTNLNPLMFALPHHMQSFGSDTQAYKTGLGL 313

Query: 355 GSID-GDLLGVVGDSWDL-------NFNPVPITWHSINGIDKKFFPEIVAALKRDVAT-- 414
            S   G +      +W L           +PI W   NG    + P  +AA++   AT  
Sbjct: 314 ASTTMGIMFAYATKTWHLIEKNLPTQVGFLPIPW---NGGSNTYSPTALAAIRAACATDI 373

Query: 415 -LNVTELSSTASSYFYAKLLARAARLALIAEEVDNGAGVIPAVVRFLKNGIQPWLIGKFP 474
             +V   S+  S Y   K++A  A++ L+A  +   + +    +  LK       + +F 
Sbjct: 374 NFDVVNASNLDSMYTSGKIVAMYAQVCLVASRILGDSTLTNTGLTKLKQA-----MARFT 433

Query: 475 KN----GFLYERKWAGLVTKNGATSTTEDFGFGIYNDHHFHLGYFVYSIAVLAKLDPNWG 534
            N      +Y+  + G+++  G +S   D+G   YNDHHFH GY +Y+ AV+  LDP+W 
Sbjct: 434 TNTQMYPLVYDTTYKGIISTAGYSSPLADYGNTYYNDHHFHWGYHIYACAVIGLLDPSWL 493

Query: 535 KQFKPQ-AYALVYDYMNFRPKKSQFSIPFRNFDFWKLHSWAAGLTEFPDGRNQESTSEAV 594
                +   AL+ D  N     + F++ FRNFD++  HSWA G+ E  DG+++ESTSE  
Sbjct: 494 VNDNIRYVNALLRDSANPSESDTYFAM-FRNFDWFVGHSWATGIFESGDGKDEESTSEDF 553

Query: 595 NAYYAAALMGLAYDDESLTAAGSTLTAAEIAATQTWWHVKSEKNWIYDKGFEEENRVVGI 654
           N  YA  L G+  +D  L    + + A    +  T+ ++    +          N V GI
Sbjct: 554 NFLYATKLWGMVRNDTVLINRANLMLAVLKNSLNTYIYMTPTTS--VQPSQILGNYVTGI 613

Query: 655 LWSAARESRLWFAPAEWRECRVGIQVLPVLPVSERVFSDKGFVKEVVEWVSR-ALEREDA 681
            +    +   +F+  E+  C+ GI ++P  P+S  + S   +V++  +W ++ A      
Sbjct: 614 TFMNKVDYATYFSAEEY--CKQGIHMIPTTPISGYLRS-PSYVQQ--DWNAKIAPIINTF 672

BLAST of Bhi06G000138 vs. Swiss-Prot
Match: sp|P53753|ENG1_YEAST (Endo-1,3(4)-beta-glucanase 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DSE4 PE=1 SV=1)

HSP 1 Score: 156.4 bits (394), Expect = 1.2e-36
Identity = 167/684 (24.42%), Postives = 291/684 (42.54%), Query Frame = 0

Query: 55   PQTTSTAVPDPAKFFSPTLLSSPLPTNSFFQNFVLNNGDKPEYIHPYIVRTANSSLSVSY 114
            P T     P+P           P+ TN F+ N ++ + + P +++PY +    SS   SY
Sbjct: 411  PPTVFDRSPNPMSLADGVSNDGPIQTNKFYTNLIVGSQESPAFVYPYSLWKYTSS---SY 470

Query: 115  PSRISNSSITQLQF-------YPDLTISALDRTSPVSNTTHFISSFSDLGVDLDIGDFR- 174
               + ++++ Q  +         +  ++ L     V + ++F SS +    ++ +   R 
Sbjct: 471  GFAVQHTTVDQYSYGGYDSSGNAEYLVNPLGIAHVVFSASNFDSSMTMQVDEMTLSSTRV 530

Query: 175  -------------FHLVRGSPYLTFSVLKISSVSISTGNGVRSVDSYDDST------KHI 234
                           LV+G  + T       +  I +  G  ++ S   S       K+ 
Sbjct: 531  VLSESNDSSNYLEIPLVQGMGFATGIYHGSLNAKIGSSVGFNTIVSESSSNLAQGILKYR 590

Query: 235  IRLNNGRNWVLY-------SSSAIYLIKSKNNQIVTSGGFSG-VIRIAVLPNSAMESGNI 294
            I L NG  W+ Y       +S+   L  S   +I  S    G +I++AV P S  +    
Sbjct: 591  ITLLNGVTWLCYVIGPDDLTSTDFSLEVSSEYEIKASASVDGLIIQLAVAP-SETDYEVF 650

Query: 295  LDRYSGCYPVSGYVK-----LSENFGFKYKWQ-KKGSGGLLMLAHPLHRQILS------R 354
             D+ +G Y  +  ++      +  + F Y  Q +  SG  ++ A P H    S       
Sbjct: 651  YDQAAGMYVTNFKLQGVSDGSTATYEFSYTTQGESASGSTMIFALPHHESSFSDIMQDYY 710

Query: 355  NQTVLPNLRYGSIDGDLLGVVGDSWDLNFNPVPITWHSINGID----KKFFPEIVAALKR 414
                L +   G ++G L   +  S  LN     + W S  G +     K   +++A +  
Sbjct: 711  TGIQLASTTKGVMNGYLTTSLQFSTSLNRQISWLPWSSQLGSNLLEYSKEQLQLLAEVAN 770

Query: 415  DVATLNVTELSSTASSYFYAKLLARAARLALIAEEVDNGAGVIPAVVRFLKNGIQPWLIG 474
                ++++E  S  ++Y+  K++ + + + L   E+        + +  +K+     L+ 
Sbjct: 771  SELQVSISESISGLNTYYLGKVIDKYSYILLTVSEIIQDEASTKSTLENIKSAFD-ILLQ 830

Query: 475  KFPKNGFLYERKWAGLVTKN--GATSTTEDFGFGIYNDHHFHLGYFVYSIAVL----AKL 534
                   +Y+ K+ GLV+    G+TST  DFG   YNDHHFH GY +++ AV+    +KL
Sbjct: 831  NEQTYPLIYDTKFNGLVSSGDWGSTSTQYDFGNTYYNDHHFHYGYIIHAAAVIGYVDSKL 890

Query: 535  DPNWGKQFKPQAYALVYDYMNFRPKKSQFSIPFRNFDFWKLHSWAAGLTEFPDGRNQEST 594
            +  W    K    +LV D  N   +K ++    R FD++  HSWAAGL E  +G+N+ES+
Sbjct: 891  NGTWAADNKDWVNSLVRDVAN-PSEKDEYFAQSRMFDWFNGHSWAAGLYENGNGKNEESS 950

Query: 595  SEAVNAYYAAALMGLAYDDESLTAAGSTLTAAEIAATQTWWHVKSEKNWIYDKGFEEE-- 654
            SE  N  YA  L G    D+S+   G  + +    A   +++ ++      D   E E  
Sbjct: 951  SEDYNFAYAMKLWGATIGDQSMELRGDLMISIMKDAMNDYFYYQN------DNTVEPEEI 1010

Query: 655  --NRVVGILWSAARESRLWFAPAEWRECRVGIQVLPVLPVSERVFSDKGFVKEVVEWVSR 677
              N+V GIL+    +   +F      E   GI +LP+ PVS  + S+  FV+E  EW ++
Sbjct: 1011 IGNKVSGILFDNIIDYTTYFGTN--TEYIHGIHMLPITPVSSNIRSET-FVEE--EWQTK 1070

BLAST of Bhi06G000138 vs. Swiss-Prot
Match: sp|Q12168|ENG2_YEAST (Endo-1,3(4)-beta-glucanase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ACF2 PE=1 SV=1)

HSP 1 Score: 154.5 bits (389), Expect = 4.6e-36
Identity = 173/666 (25.98%), Postives = 290/666 (43.54%), Query Frame = 0

Query: 77  PLPTNSFFQNFVLNNGDKPEYIHPYIV----------RTANSSLSVSYPSRISNSSITQL 136
           PL TN F+ N +L++  +P + HPY +            AN +L+     R+ +++    
Sbjct: 110 PLETNKFYTNMLLDDNTQPIWTHPYSIWFSRDPELFGLAANHTLA---SQRVFDTTTNPP 169

Query: 137 QFYPDLTISALDRTSPVSNTTHFISS------FSD-------LGVDLDIGDF-RFHLVRG 196
           +FY     +  +  S V     F+SS      F D       L + L    F  F LV+G
Sbjct: 170 RFY----FNPTNIKSFVFKAREFVSSNDIKLEFRDMKHMSMCLLMSLSSSQFIEFPLVQG 229

Query: 197 SPYLTFSVLKISSVSISTGNGVRSVDSYDDST---KHIIRLNNGRNWVLYSSSAIYLIKS 256
             ++T ++       + +  G RS++    +    K+ I+L N RNW+LY +S  Y    
Sbjct: 230 MGFVT-AIYHDLGFELRSAVGFRSLERISVNERYGKYNIQLENNRNWILYLTSPDYSFPQ 289

Query: 257 -------KNNQIVTSGGFSGVIRIAVLPNSAMESGNILDRYSGCYPVSGYVKLS-----E 316
                   +N I++S   +G+I        + +S   +D  +GCYPV  Y  LS     E
Sbjct: 290 DFQISLLDSNTIISSHKINGLI-----CQLSADSVPSIDMAAGCYPV--YCDLSGQTVDE 349

Query: 317 NF-GFKYKWQKKG---SGGLLMLAHPLH----------RQILSRNQTVLPNLRYGSIDGD 376
           +F  +++ +   G   SG  LM A P H          R+I S   + +  L  G +   
Sbjct: 350 HFTNYRFNYTVAGYSQSGTTLMYALPHHKAAFTPEMQEREIASSLDSTVKGLMTGYLTNS 409

Query: 377 LLGVVGDSWDLNFNPVPITWHSINGIDKKFFPEIVAALKRDVATLNVTELSSTASSYFYA 436
               V    +L F PV ++ +      ++   +I  A  ++V   +  + S+  S YF  
Sbjct: 410 FDMQVQVPQELGFEPVALSLNKKADYSQEKLSKIREAAVQEVQLSDPQQESNIDSMYFSG 469

Query: 437 KLLARAARLALIAEEVDNGAGVIPAVVRFLKNGIQPWLIGK--FPKNGFLYERKWAGLVT 496
           K+LA+ A +  +   + +   +   ++  L   ++ ++  +   P N   Y+  W G+++
Sbjct: 470 KILAKYAWILYVTHYILHDENLTKELLSKLTIAMERFISNQQVLPLN---YDVSWKGIIS 529

Query: 497 KNGATSTTEDFGFGIYNDHHFHLGYFVYSIAVLAKLDP--------NWGKQFKPQAYALV 556
                S+++DFG   YNDHHFH  Y V + A+++ +D         +W +  +     L+
Sbjct: 530 SG---SSSQDFGNSYYNDHHFHYSYHVITAAIISLVDSDLSGVTNNSWLENNRDWVECLI 589

Query: 557 YDYMNFRPKKSQFSIPFRNFDFWKLHSWAAGLTEFPDGRNQESTSEAVNAYYAAALMGLA 616
            DY         F   FR+FD++  HSWA GL    DG+++ESTSE VN+ YA  L GL 
Sbjct: 590 RDYSGVDNDDPYFP-QFRSFDWFNGHSWAKGLFPSGDGKDEESTSEDVNSCYAIKLWGLV 649

Query: 617 YDDESLTAAGSTLTAAEIAATQTWWHVKSEKNWIYDKGFEEENRVVGILWSAARESRLWF 676
             +  LT   +          Q+++  +S  N +  K F   N+V GIL+    +   +F
Sbjct: 650 TGNSKLTDIANLQLGIMRNVFQSYFLYES-NNTVQPKEF-IGNKVSGILFENKIDHATYF 709

Query: 677 APAEWRECRVGIQVLPVLPVSERVFSDKGFVKEVVEWVSRALERED-AGEGWKGFAYALE 679
              E +   + I  +P+   S  V     FVKE  EW  +     D   +GWKG      
Sbjct: 710 G-MEPQYIHM-IHAIPITSASSWV-RTPNFVKE--EWEEKMQPIIDQVNDGWKGIIMLNM 746

BLAST of Bhi06G000138 vs. Swiss-Prot
Match: sp|Q9UT45|ENG1_SCHPO (Endo-1,3(4)-beta-glucanase 1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=eng1 PE=3 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 7.8e-28
Identity = 124/508 (24.41%), Postives = 219/508 (43.11%), Query Frame = 0

Query: 205 KHIIRLNNGRNWVLY-SSSAIYLIKSKNNQIVTSGGFSGVIRIAVLPNSAMESGNILDRY 264
           K+ + +++   W++Y    ++ L +S +  +V S  F+G I+IA +P     +  + D Y
Sbjct: 226 KYRVTMSDNHVWLIYIYGDSLTLTESTSQMLVGSNTFNGYIQIAKIPLGDGTAEALYDTY 285

Query: 265 SGCY----PVSGYVKLSENFGFKYKWQKKGSGGL----LMLAHPLHRQILSRNQT--VLP 324
           +G Y     +SGYV+ +  + + + +   G   +     +L H +   +     T  VL 
Sbjct: 286 AGVYITGISISGYVEDAVGY-YSFDFTTAGDTSVEPLFFLLPHQVDTAVSGTKVTSIVLA 345

Query: 325 NLRYGSIDGDLLGVVGDS--------WDLNFNPVPITWHSINGIDKKFFPEIVAALKRDV 384
           +L    + GD+    G+S         D+ F P   T   I   ++    EI+A +    
Sbjct: 346 SL----VSGDMNAAAGNSITFAEAIPQDIGFLPWSPTGGQIGYSEEAL--EIIAEVAGTE 405

Query: 385 ATLNVTELSSTASSYFYAKLLARAARLALIAEEVDNGAGVIPAVVRFLKNGIQPWLIGK- 444
              + +  S+  S Y+  K+LA+ A L +   ++          ++ L+     ++  + 
Sbjct: 406 LGEDFSANSNLNSMYYSGKVLAKYAMLCVTINDILGDETSSEQCIQKLEAAFARFVDNQQ 465

Query: 445 -FPKNGFLYERKWAGLVTKNGAT-STTEDFGFGIYNDHHFHLGYFVYSIAVLAKLDPNWG 504
            +P     Y+  W G+V+  G +  +  DFG   YNDHHFH GYFV++ AV+  +DP+W 
Sbjct: 466 IYP---LTYDNTWKGVVSVAGLSGDSLADFGNSYYNDHHFHYGYFVFTAAVIGHIDPDWI 525

Query: 505 KQFKPQAYA--LVYDYMNFRPKKSQFSIP-FRNFDFWKLHSWAAGLTEFPDGRNQESTSE 564
                + +   LV D  N  P  +    P  R  D +  H WA+GL E  DG+++ESTSE
Sbjct: 526 NTGNNKEWVNFLVRDVAN--PSSNDPYFPKHRMIDIYHGHGWASGLFESNDGKDEESTSE 585

Query: 565 AVNAYYAAALMGLAYDDESLTAAGSTLTAAEIAATQTWWHVKSEKNWIYDKG-----FEE 624
             N ++   L G    D  +    + +   E  A          K  +Y  G       +
Sbjct: 586 DYNFFFGMKLWGQVIGDSDMEDRANIILGIERNAL--------NKYMLYADGNVQPTSMQ 645

Query: 625 ENRVVGILWSAARESRLWFAPAEWRECRVGIQVLPVLPVSERVFSDKGFVKEVVEWVSRA 683
            N V GI +        +F      E   GI +LP+ P+S  +      + E    ++  
Sbjct: 646 PNYVAGITFMNKITHTTYFGTN--IEYIQGIHMLPITPISAFIRGPSFVLAEWNALLASV 705

BLAST of Bhi06G000138 vs. TAIR10
Match: AT5G15870.1 (glycosyl hydrolase family 81 protein)

HSP 1 Score: 731.1 bits (1886), Expect = 6.5e-211
Identity = 379/656 (57.77%), Postives = 468/656 (71.34%), Query Frame = 0

Query: 52  FLFPQTTSTAVPDPAKFFSPTLLSSPLPTNSFFQNFVLNNGDKPEYIHPYIVRTANSSLS 111
           FLFP++ S+ +PDP++FFS  LLSSPLPTNSFFQNF LNNGD+ EY HPYI++ + SSLS
Sbjct: 55  FLFPRSDSSVLPDPSRFFSHDLLSSPLPTNSFFQNFTLNNGDQAEYFHPYIIKPSTSSLS 114

Query: 112 VSYPSRISNSSITQLQFYPDLTISALDRTSPVSNTTHFISSFSDLGVDLDI--GDFRFHL 171
           +SYPS   NS+     F  D+TI+  D   P S  +H ISSFSDLGV LD    + RF L
Sbjct: 115 ISYPSLSHNSAFIYEAFNADITITGSDGPDPHSRKSHLISSFSDLGVTLDFPSSNLRFFL 174

Query: 172 VRGSPYLTFSVLKISSVSISTGNGVRSVDSYDDSTKHIIRLNNGRNWVLYSSSAIYLIKS 231
           VRGSP++TFSV   SS++IST + V S+     STK+ ++LNN + W++Y+SS I L K 
Sbjct: 175 VRGSPFITFSV--NSSITISTIHAVLSLSGNTSSTKYTVKLNNNQTWLIYASSPINLTKD 234

Query: 232 KNNQIVTSGGFSGVIRIAVLPNSAMESGNILDRYSGCYPVSGYVKLSENFGFKYKWQKKG 291
             + I    GFSG+IRI VLPN       ILD +S  YPVSG    ++ F  +YKW+K+G
Sbjct: 235 GVSSINCGDGFSGIIRIVVLPNPNPYFETILDGFSCSYPVSGDADFTKPFALEYKWEKRG 294

Query: 292 SGGLLMLAHPLHRQILSRNQ---TVLPNLRYGSIDGDLLGVVGDSWDLNFNPVPITWHSI 351
            G LLMLAHPLH ++LS N    TVL N +Y SIDGDL+GV+GDSW L  +PV +TWHSI
Sbjct: 295 YGDLLMLAHPLHLKLLSTNDCSITVLDNFKYNSIDGDLVGVIGDSWVLKPDPVSVTWHSI 354

Query: 352 NGIDKKFFPEIVAALKRDVATLNVTELSSTASSYFYAKLLARAARLALIAEEVDNGAGVI 411
            G+ +    EI++AL +DV  L+ +    T SSYFYAKL+ARAARLALIAEEV     VI
Sbjct: 355 KGVQEDSHQEIISALIKDVNALD-SSAEVTNSSYFYAKLIARAARLALIAEEVCY-LDVI 414

Query: 412 PAVVRFLKNGIQPWLIGKFPKNGFLYERKWAGLVTKNGATSTTEDFGFGIYNDHHFHLGY 471
           P +  +LKN I+PWL G F  NGFLY+ KW G++TK G+  +  DFGFGIYNDHH+HLGY
Sbjct: 415 PKIRTYLKNMIEPWLNGSFGPNGFLYDPKWGGVITKLGSRDSGADFGFGIYNDHHYHLGY 474

Query: 472 FVYSIAVLAKLDPNWGKQFKPQAYALVYDYMNFRPK--KSQFSIP-FRNFDFWKLHSWAA 531
           FVY+IAVLAK+DP WGK+++PQAY L+ DY+    K  KS  + P  R FD +KLHSWA 
Sbjct: 475 FVYAIAVLAKIDPLWGKRYRPQAYTLMADYLTLGKKGAKSNSNYPRLRCFDLFKLHSWAG 534

Query: 532 GLTEFPDGRNQESTSEAVNAYYAAALMGLAYDDESLTAAGSTLTAAEIAATQTWWHVKSE 591
           GLTEF DGRNQESTSEAVNAYY+AAL+GLAY D  L AA S +   EI A + WW VK E
Sbjct: 535 GLTEFADGRNQESTSEAVNAYYSAALLGLAYGDTHLVAAASMVLTLEIHAAKMWWQVK-E 594

Query: 592 KNWIYDKGFEEENRVVGILWSAARESRLWFAPAEWRECRVGIQVLPVLPVSERVFSDKGF 651
            + IY + F  ENRVVG+LWS  R+S LWFAP EW+ECR+GIQ+LP+LPVSE +FSD  F
Sbjct: 595 DDAIYPQDFTSENRVVGVLWSTKRDSGLWFAPKEWKECRLGIQLLPLLPVSEVLFSDVTF 654

Query: 652 VKEVVEWVSRALEREDAGEGWKGFAYALEGIYDKKSAIEKVKKLKKHDDGNSLSNL 700
           VK++V W   AL R+  GEGWKGF YALE +YDK  A+EK+K L   DDGNSLSNL
Sbjct: 655 VKQLVNWTMPALARDSVGEGWKGFVYALESMYDKDGAMEKIKGLNGFDDGNSLSNL 705

BLAST of Bhi06G000138 vs. TAIR10
Match: AT1G18310.1 (glycosyl hydrolase family 81 protein)

HSP 1 Score: 680.6 bits (1755), Expect = 1.0e-195
Identity = 355/684 (51.90%), Postives = 446/684 (65.20%), Query Frame = 0

Query: 52  FLFPQTTSTAVPDPAKFFSPTLLSSPLPTNSFFQNFVLNNGDKPEYIHPYIVRTANSSLS 111
           FLFP+T S+ +PDP++FFSP LLS+PLPTNSFFQNF L NGD+ EY HPY++++ +SSL 
Sbjct: 8   FLFPETKSSVLPDPSRFFSPDLLSNPLPTNSFFQNFTLKNGDQAEYFHPYLIKSVDSSLC 67

Query: 112 VSYPSRISNSSITQLQFYPDLTISALDRTSPVSNTTHFISSFSDLGVDLDI--GDFRFHL 171
           +SYPS   NS      F  D+ IS  +   P S  TH ISSFSDL V LD    + RF L
Sbjct: 68  ISYPSLFHNSDFFYEVFTADIIISGSNGPGPRSRKTHIISSFSDLVVTLDFPSSNLRFFL 127

Query: 172 VRGSPYLTFSVLKISSVSISTGNGVRSVDSYDDSTKHIIRLNNGRNWVLYSSSAIYLIKS 231
           VRGSP                            STK   +L N + W++Y+SS I L K 
Sbjct: 128 VRGSP--------------------------SSSTKWSDKLTNNQRWLIYASSPIDLTKH 187

Query: 232 KNNQIVTSGGFSGVIRIAVLPNSAMESGNILDRYSGCYPVSGYVKLSENFGFKYKWQKKG 291
            ++ I   GGF+G++RI VLP S     + LDR+S CYPVSG    ++ F  KY W+K+G
Sbjct: 188 GDSSIHCRGGFTGIVRITVLPGSNPGYESTLDRFSSCYPVSGDADFTKPFTLKYMWEKRG 247

Query: 292 SGGLLMLAHPLHRQILSRNQ-----------------------TVLPNLRYGSIDGDLLG 351
           SG LLMLAHPLH ++L+++                        TVL + RY SIDGDL+G
Sbjct: 248 SGDLLMLAHPLHLKLLAKDASSLFQQSTLLLVCPDRGCTNSSVTVLDHFRYKSIDGDLVG 307

Query: 352 VVGDSWDLNFNPVPITWHSINGIDKKFFPEIVAALKRDVATLNVTELSSTASSYFYAKLL 411
           VVGDSW L  + V +TWHS+ G+ K  + EI++AL +DV  LN +    T+SSYFY KL+
Sbjct: 308 VVGDSWVLKPDSVSVTWHSMKGVKKDSYKEIISALGKDVNGLN-SSAEVTSSSYFYGKLI 367

Query: 412 ARAARLALIAEEVDNGAGVIPAVVRFLKNGIQPWLIGKFPKNGFLYERKWAGLVTKNGAT 471
           ARAAR ALIAEEV     VIP +V +LKN I+PWL G F  NGFLY+ KW GL+TK G+ 
Sbjct: 368 ARAARFALIAEEVCY-LDVIPKIVTYLKNMIEPWLDGSFKPNGFLYDPKWGGLITKQGSK 427

Query: 472 STTEDFGFGIYNDHHFHLGYFVYSIAVLAKLDPNWGKQFKPQAYALVYDYMNFRPK---K 531
            +  DFGFGIYNDHH+H+GYF+Y+IAVLAK DP WG++++ QAY+L+ D+M F  K    
Sbjct: 428 DSQADFGFGIYNDHHYHIGYFLYAIAVLAKFDPLWGERYRAQAYSLLADFMTFGRKDDNN 487

Query: 532 SQFSIP-FRNFDFWKLHSWAAGLTEFPDGRNQESTSEAVNAYYAAALMGLAYDDESLTAA 591
           S  S P  RNFD +KLHSWA GLTEF DGRNQESTSEAVNAYY+AAL+GLAY D+ L   
Sbjct: 488 SNSSYPRLRNFDLFKLHSWAGGLTEFWDGRNQESTSEAVNAYYSAALLGLAYGDKHLVET 547

Query: 592 GSTLTAAEIAATQTWWHVKSEKNWIYDKGFEEENRVVGILWSAARESRLWFAPAEWRECR 651
            ST+   EI A + WW VK  +  +Y K F  ENRVVG+LWS  R+S LWF P EW+ECR
Sbjct: 548 ASTIMTLEIHAAKMWWQVKKGEA-LYPKDFTAENRVVGVLWSTKRDSSLWFGPKEWKECR 607

Query: 652 VGIQVLPVLPVSERVFSDKGFVKEVVEWVSRALEREDAGEGWKGFAYALEGIYDKKSAIE 707
           +GIQ+LP+LP              +V W   AL+R   GEGWKGF YALE +YDK  AI+
Sbjct: 608 LGIQLLPILP--------------LVNWTLPALQRNGVGEGWKGFLYALESLYDKDGAIK 648

BLAST of Bhi06G000138 vs. TrEMBL
Match: tr|A0A0A0KW06|A0A0A0KW06_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G647400 PE=4 SV=1)

HSP 1 Score: 1213.0 bits (3137), Expect = 0.0e+00
Identity = 600/687 (87.34%), Postives = 629/687 (91.56%), Query Frame = 0

Query: 24  ETPKPWLISIFXXXXXXXTAFPPAVSGQFLFPQTTSTAVPDPAKFFSPTLLSSPLPTNSF 83
           ET KP  IS          A   AVS QF FP+TTSTAVPDP KFFSP LLSSPLPTNSF
Sbjct: 3   ETSKPSPISTLFFLLLLTAA---AVSAQFPFPETTSTAVPDPGKFFSPNLLSSPLPTNSF 62

Query: 84  FQNFVLNNGDKPEYIHPYIVRTANSSLSVSYPSRISNSSITQLQFYPDLTISALDRTSPV 143
           FQNFVLNNGD PEYIHPY++RTANSSLSVSYPSRISNSSITQLQF+PDL IS+  +T   
Sbjct: 63  FQNFVLNNGDLPEYIHPYLIRTANSSLSVSYPSRISNSSITQLQFFPDLVISSPKKT--- 122

Query: 144 SNTTHFISSFSDLGVDLDIGDFRFHLVRGSPYLTFSVLKISSVSISTGNGVRSVDSYDDS 203
            NTTHFISSFSDLGVDLDIG FRFHLVRGSPYLTFSVLK SSV IST NGVRSVDSY+D 
Sbjct: 123 FNTTHFISSFSDLGVDLDIGVFRFHLVRGSPYLTFSVLKTSSVLISTSNGVRSVDSYEDY 182

Query: 204 TKHIIRLNNGRNWVLYSSSAIYLIKSKNNQIVTSGGFSGVIRIAVLPNSAMESGNILDRY 263
           TKHIIRLNNGR+WVLYSSSAIYL+KSK+NQIVTSGGF GVIR+AVLP+SA+ES  ILDRY
Sbjct: 183 TKHIIRLNNGRSWVLYSSSAIYLVKSKSNQIVTSGGFIGVIRVAVLPDSAVESEKILDRY 242

Query: 264 SGCYPVSGYVKLSENFGFKYKWQKKGSGGLLMLAHPLHRQILSRNQTVLPNLRYGSIDGD 323
           SGCYPVSG+VKL   FGF+YKWQKKGSGGLLMLAH LHR+IL R+QTVL N+RY SIDGD
Sbjct: 243 SGCYPVSGFVKLLGGFGFEYKWQKKGSGGLLMLAHTLHREILPRDQTVLQNIRYSSIDGD 302

Query: 324 LLGVVGDSWDLNFNPVPITWHSINGIDKKFFPEIVAALKRDVATLNVTELSSTASSYFYA 383
           LLGVVGDSWDL FNP+PITWHSINGID KFFPEIVAALKRDVATLN TELSS  +SYFY 
Sbjct: 303 LLGVVGDSWDLKFNPIPITWHSINGIDSKFFPEIVAALKRDVATLNATELSSMPASYFYG 362

Query: 384 KLLARAARLALIAEEVDNGAGVIPAVVRFLKNGIQPWLIGKFPKNGFLYERKWAGLVTKN 443
           KLLARAARLALIAEEV+ GAGVIPAVV+FLKNGIQPWLIGKFPKNGFLYERKWAGLVTKN
Sbjct: 363 KLLARAARLALIAEEVNGGAGVIPAVVKFLKNGIQPWLIGKFPKNGFLYERKWAGLVTKN 422

Query: 444 GATSTTEDFGFGIYNDHHFHLGYFVYSIAVLAKLDPNWGKQFKPQAYALVYDYMNFRPKK 503
           GATSTTEDFGFGIYNDHHFHLGYFVYSIAVLAKLDPNWGKQ+KPQAYAL+YDYMNFRPKK
Sbjct: 423 GATSTTEDFGFGIYNDHHFHLGYFVYSIAVLAKLDPNWGKQYKPQAYALLYDYMNFRPKK 482

Query: 504 SQFSIPFRNFDFWKLHSWAAGLTEFPDGRNQESTSEAVNAYYAAALMGLAYDDESLTAAG 563
           SQFSIPFRNFDFWKLHSWAAGLTEFPDGRNQEST+EAVNAYYAAALMGLAYDDESLTAAG
Sbjct: 483 SQFSIPFRNFDFWKLHSWAAGLTEFPDGRNQESTTEAVNAYYAAALMGLAYDDESLTAAG 542

Query: 564 STLTAAEIAATQTWWHVKSEKNWIYDKGFEEENRVVGILWSAARESRLWFAPAEWRECRV 623
           STLTAAEI ATQTWWHVK E N IYDKGF EENR+VGILWSAARESRLWFAPAEWRECRV
Sbjct: 543 STLTAAEITATQTWWHVKRENNGIYDKGFTEENRMVGILWSAARESRLWFAPAEWRECRV 602

Query: 624 GIQVLPVLPVSERVFSDKGFVKEVVEWVSRALEREDAGEGWKGFAYALEGIYDKKSAIEK 683
           GIQVLPVLPV+ERVF+D GFVKEVVEWV  ALEREDAGEGWKGFAYALEGIYDKKSA+EK
Sbjct: 603 GIQVLPVLPVTERVFTDVGFVKEVVEWVQPALEREDAGEGWKGFAYALEGIYDKKSAVEK 662

Query: 684 VKKLKKHDDGNSLSNLLWWIYSRPEGR 711
           VKKLKKHDDGNSLSNLLWWIYSRPEGR
Sbjct: 663 VKKLKKHDDGNSLSNLLWWIYSRPEGR 683

BLAST of Bhi06G000138 vs. TrEMBL
Match: tr|A0A1S3C9V5|A0A1S3C9V5_CUCME (putative endo-1,3(4)-beta-glucanase 2 OS=Cucumis melo OX=3656 GN=LOC103498635 PE=4 SV=1)

HSP 1 Score: 1197.6 bits (3097), Expect = 0.0e+00
Identity = 602/714 (84.31%), Postives = 638/714 (89.36%), Query Frame = 0

Query: 1   MTFHNPPFYNYKILSTNNPSLAME---TPKPWLISIFXXXXXXXTAFPPAVSGQFLFPQT 60
           M FHN PF     LS    SLAME      P+ I  F       TA P  VS QF FP+T
Sbjct: 1   MAFHN-PFPQSFFLS----SLAMEETCKQSPFSILFF---FLLLTAAP--VSAQFPFPET 60

Query: 61  TSTAVPDPAKFFSPTLLSSPLPTNSFFQNFVLNNGDKPEYIHPYIVRTANSSLSVSYPSR 120
           TSTAVPDP+KFFSP LLSSPLPTNSFFQNFVLNNGD PEYIHPY++RTANSSL VSYPSR
Sbjct: 61  TSTAVPDPSKFFSPNLLSSPLPTNSFFQNFVLNNGDSPEYIHPYLIRTANSSLFVSYPSR 120

Query: 121 ISNSSITQLQFYPDLTISALDRTSPVSNTTHFISSFSDLGVDLDIGDFRFHLVRGSPYLT 180
           ISNSSI QLQF+PDL IS+L++T   SN THF+SSFSDLGVDLDIG FR+HLVRGSPYLT
Sbjct: 121 ISNSSINQLQFFPDLVISSLNKT---SNATHFVSSFSDLGVDLDIGVFRYHLVRGSPYLT 180

Query: 181 FSVLKISSVSISTGNGVRSVDSYDDSTKHIIRLNNGRNWVLYSSSAIYLIKSKNNQIVTS 240
           FSVLK S V IST NGVRSVDSY+D TK+IIRLNNGR+WVLYSSSAIYLIKSK+NQIVTS
Sbjct: 181 FSVLKTSFVLISTSNGVRSVDSYEDYTKYIIRLNNGRSWVLYSSSAIYLIKSKSNQIVTS 240

Query: 241 GGFSGVIRIAVLPNSAMESGNILDRYSGCYPVSGYVKLSENFGFKYKWQKKGSGGLLMLA 300
           GGF GVIR+AVLP+SA+ES  ILDRYSGCYPVSG+ KLS  FGF+YKWQKKGSGGLLMLA
Sbjct: 241 GGFIGVIRVAVLPDSAVESEKILDRYSGCYPVSGFAKLSGGFGFEYKWQKKGSGGLLMLA 300

Query: 301 HPLHRQILSRNQTVLPNLRYGSIDGDLLGVVGDSWDLNFNPVPITWHSINGIDKKFFPEI 360
           H LHR+IL R+QTVL NLRY SIDGDLLGVVGDSWDL FNP+PITWHSINGI+ KFFPEI
Sbjct: 301 HTLHREILPRDQTVLQNLRYSSIDGDLLGVVGDSWDLKFNPIPITWHSINGINSKFFPEI 360

Query: 361 VAALKRDVATLNVTELSSTASSYFYAKLLARAARLALIAEEVDNGAGVIPAVVRFLKNGI 420
           VAALKRDVATLNVTELSST +SYFY KLLARAARLALIAEEV+ GA VIPAVV+FLKNGI
Sbjct: 361 VAALKRDVATLNVTELSSTPASYFYGKLLARAARLALIAEEVNYGASVIPAVVKFLKNGI 420

Query: 421 QPWLIGKFPKNGFLYERKWAGLVTKNGATSTTEDFGFGIYNDHHFHLGYFVYSIAVLAKL 480
           QPWLIGKFPKNGFLYERKWAGLVTKNGATST EDFGFGIYNDHHFHLGYFVYSIAVLAKL
Sbjct: 421 QPWLIGKFPKNGFLYERKWAGLVTKNGATSTIEDFGFGIYNDHHFHLGYFVYSIAVLAKL 480

Query: 481 DPNWGKQFKPQAYALVYDYMNFRPKKSQFSIPFRNFDFWKLHSWAAGLTEFPDGRNQEST 540
           DPNW KQ+KPQAYAL+YDYMNFRPKKSQFSIPFRNFDFWKLHSWAAGL EFPDGRNQEST
Sbjct: 481 DPNWRKQYKPQAYALLYDYMNFRPKKSQFSIPFRNFDFWKLHSWAAGLAEFPDGRNQEST 540

Query: 541 SEAVNAYYAAALMGLAYDDESLTAAGSTLTAAEIAATQTWWHVKSEKNWIYDKGFEEENR 600
           SEAVNAYYAAALMGLAY D SLTAAGSTLTAAEI A+QTWWHVK EKN IYDKGF EENR
Sbjct: 541 SEAVNAYYAAALMGLAYGDASLTAAGSTLTAAEITASQTWWHVKKEKNGIYDKGFAEENR 600

Query: 601 VVGILWSAARESRLWFAPAEWRECRVGIQVLPVLPVSERVFSDKGFVKEVVEWVSRALER 660
           +VGILWSAARESRLWFAPAEWRECR+GIQVLPVLPV+ER+F+D GFVKE VEWV  ALER
Sbjct: 601 MVGILWSAARESRLWFAPAEWRECRLGIQVLPVLPVTERMFTDVGFVKEAVEWVQPALER 660

Query: 661 EDAGEGWKGFAYALEGIYDKKSAIEKVKKLKKHDDGNSLSNLLWWIYSRPEGRG 712
           EDAGEGWKGFAYALEGIYDKKSA+EKVKKLKKHDDGNSLSNLLWWIYSRPEGRG
Sbjct: 661 EDAGEGWKGFAYALEGIYDKKSAVEKVKKLKKHDDGNSLSNLLWWIYSRPEGRG 701

BLAST of Bhi06G000138 vs. TrEMBL
Match: tr|A0A142BTQ9|A0A142BTQ9_CUCME (Beta-1,3-glucanase 2 OS=Cucumis melo OX=3656 GN=glu2 PE=2 SV=1)

HSP 1 Score: 1192.9 bits (3085), Expect = 0.0e+00
Identity = 586/670 (87.46%), Postives = 621/670 (92.69%), Query Frame = 0

Query: 42  TAFPPAVSGQFLFPQTTSTAVPDPAKFFSPTLLSSPLPTNSFFQNFVLNNGDKPEYIHPY 101
           TA P  VS QF FP+TTSTAVPDP+KFFSP LLSSPLPTNSFFQNFVLNNGD PEYIHPY
Sbjct: 20  TAAP--VSAQFPFPETTSTAVPDPSKFFSPNLLSSPLPTNSFFQNFVLNNGDSPEYIHPY 79

Query: 102 IVRTANSSLSVSYPSRISNSSITQLQFYPDLTISALDRTSPVSNTTHFISSFSDLGVDLD 161
           ++RTANSSL VSYPSRISNSSI QLQF+PDL IS+L++T   SN THF+SSFSDLGVDLD
Sbjct: 80  LIRTANSSLFVSYPSRISNSSINQLQFFPDLVISSLNKT---SNATHFVSSFSDLGVDLD 139

Query: 162 IGDFRFHLVRGSPYLTFSVLKISSVSISTGNGVRSVDSYDDSTKHIIRLNNGRNWVLYSS 221
           IG FR+HLVRGSPYLTFSVLK S V IST NGVRSVDSY+D TK+IIRLNNGR+WVLYSS
Sbjct: 140 IGVFRYHLVRGSPYLTFSVLKTSFVLISTSNGVRSVDSYEDYTKYIIRLNNGRSWVLYSS 199

Query: 222 SAIYLIKSKNNQIVTSGGFSGVIRIAVLPNSAMESGNILDRYSGCYPVSGYVKLSENFGF 281
           SAIYLIKSK+NQIVTSGGF GVIR+AVLP+SA+ES  ILDRYSGCYPVSG+ KLS  FGF
Sbjct: 200 SAIYLIKSKSNQIVTSGGFIGVIRVAVLPDSAVESEKILDRYSGCYPVSGFAKLSGGFGF 259

Query: 282 KYKWQKKGSGGLLMLAHPLHRQILSRNQTVLPNLRYGSIDGDLLGVVGDSWDLNFNPVPI 341
           +YKWQKKGSGGLLMLAH LHR+IL R+QTVL NLRY SIDGDLLGVVGDSWDL FNP+PI
Sbjct: 260 EYKWQKKGSGGLLMLAHTLHREILPRDQTVLQNLRYSSIDGDLLGVVGDSWDLKFNPIPI 319

Query: 342 TWHSINGIDKKFFPEIVAALKRDVATLNVTELSSTASSYFYAKLLARAARLALIAEEVDN 401
           TWHSINGI+ KFFPEIVAALKRDVATLNVTELSST +SYFY KLLARAARLALIAEEV+ 
Sbjct: 320 TWHSINGINSKFFPEIVAALKRDVATLNVTELSSTPASYFYGKLLARAARLALIAEEVNY 379

Query: 402 GAGVIPAVVRFLKNGIQPWLIGKFPKNGFLYERKWAGLVTKNGATSTTEDFGFGIYNDHH 461
           GA VIPAVV+FLKNGIQPWLIGKFPKNGFLYERKWAGLVTKNGATST EDFGFGIYNDHH
Sbjct: 380 GASVIPAVVKFLKNGIQPWLIGKFPKNGFLYERKWAGLVTKNGATSTIEDFGFGIYNDHH 439

Query: 462 FHLGYFVYSIAVLAKLDPNWGKQFKPQAYALVYDYMNFRPKKSQFSIPFRNFDFWKLHSW 521
           FHLGYFVYSIAVLAKLDPNW KQ+KPQAYAL+YDYMNFRPKKSQFSIPFRNFDFWKLHSW
Sbjct: 440 FHLGYFVYSIAVLAKLDPNWRKQYKPQAYALLYDYMNFRPKKSQFSIPFRNFDFWKLHSW 499

Query: 522 AAGLTEFPDGRNQESTSEAVNAYYAAALMGLAYDDESLTAAGSTLTAAEIAATQTWWHVK 581
           AAGL EFPDGRNQESTSEAVNAYYAAALMGLAY D SLTAAGSTLTAAEI A+QTWWHVK
Sbjct: 500 AAGLAEFPDGRNQESTSEAVNAYYAAALMGLAYGDASLTAAGSTLTAAEITASQTWWHVK 559

Query: 582 SEKNWIYDKGFEEENRVVGILWSAARESRLWFAPAEWRECRVGIQVLPVLPVSERVFSDK 641
            EKN IYDKGF EENR+VGILWSAARESRLWFAPAEWRECR+GIQVLPVLPV+ER+F+D 
Sbjct: 560 KEKNGIYDKGFAEENRMVGILWSAARESRLWFAPAEWRECRLGIQVLPVLPVTERMFTDV 619

Query: 642 GFVKEVVEWVSRALEREDAGEGWKGFAYALEGIYDKKSAIEKVKKLKKHDDGNSLSNLLW 701
           GFVKE VEWV  ALEREDAGEGWKGFAYALEGIYDKKSA+EKVKKLKKHDDGNSLSNLLW
Sbjct: 620 GFVKEAVEWVQPALEREDAGEGWKGFAYALEGIYDKKSAVEKVKKLKKHDDGNSLSNLLW 679

Query: 702 WIYSRPEGRG 712
           WIYSRPEGRG
Sbjct: 680 WIYSRPEGRG 684

BLAST of Bhi06G000138 vs. TrEMBL
Match: tr|A0A1Q3DC08|A0A1Q3DC08_CEPFO (Glyco_hydro_81 domain-containing protein OS=Cephalotus follicularis OX=3775 GN=CFOL_v3_33201 PE=4 SV=1)

HSP 1 Score: 796.6 bits (2056), Expect = 4.6e-227
Identity = 396/668 (59.28%), Postives = 499/668 (74.70%), Query Frame = 0

Query: 46  PAVSGQFLFPQTTSTAVPDPAKFFSPTLLSSPLPTNSFFQNFVLNNGDKPEYIHPYIVRT 105
           P  +  FLFP T ST +PDP+ FFSP LLS+PLPTNSFFQNF L NGD+ EYIHPY++++
Sbjct: 51  PPPNSPFLFPNTQSTVLPDPSLFFSPALLSTPLPTNSFFQNFTLKNGDQAEYIHPYLIKS 110

Query: 106 ANSSLSVSYPSRISNSSITQLQFYPDLTISALDRTSPVSNTTHFISSFSDLGVDLDI--G 165
           + +SLSVSYPS+  NSS T   F PDLTISA D TSP S  +H I+S+SDL V LDI   
Sbjct: 111 SLTSLSVSYPSQFHNSSFTYQVFVPDLTISASDNTSPDSQRSHIITSYSDLSVTLDIPSS 170

Query: 166 DFRFHLVRGSPYLTFSVLKISSVSISTGNGVRSVDSYDDSTKHIIRLNNGRNWVLYSSSA 225
           + RF +VRGSP+LTFSV   +++SIST + + S DS    TK+ I+LNN + WVLYSSS 
Sbjct: 171 NLRFFIVRGSPFLTFSVTNNTTISISTIHAILSFDSNSSLTKYTIKLNNNQTWVLYSSSP 230

Query: 226 IYLIKSKNNQIVTSGGFSGVIRIAVLPNSAMESGNILDRYSGCYPVSGYVKLSENFGFKY 285
           I L  S +  ++TS GFSG+IRI VLP+S  +   ILDR+S CYPVSG    ++ F  +Y
Sbjct: 231 INLCNSLS--LITSDGFSGIIRIVVLPDSDPKYEAILDRFSSCYPVSGDAAFTKPFCLEY 290

Query: 286 KWQKKGSGGLLMLAHPLHRQILSRNQ---TVLPNLRYGSIDGDLLGVVGDSWDLNFNPVP 345
           +W KKG G LLMLAHPLH +ILS +    TVL + +Y SIDGDL+GV+GDSW L  + V 
Sbjct: 291 RWGKKGWGDLLMLAHPLHVKILSADDCDITVLEDFKYKSIDGDLIGVIGDSWVLKPDQVS 350

Query: 346 ITWHSINGIDKKFFPEIVAALKRDVATLNVTELSSTASSYFYAKLLARAARLALIAEEVD 405
           +TWHSI G+ ++ + EIV+AL++DV  LN T +++T SSYFY KL+ARAARLALIAEEV 
Sbjct: 351 VTWHSIEGVKEEAYDEIVSALRKDVDALNPTTITTT-SSYFYGKLIARAARLALIAEEVC 410

Query: 406 NGAGVIPAVVRFLKNGIQPWLIGKFPKNGFLYERKWAGLVTKNGATSTTEDFGFGIYNDH 465
               VIPA+ +FLK+ I+PWL G F  NGFLY+ KW G+VTK G+  +  DFGFG+YNDH
Sbjct: 411 Y-LDVIPAIRKFLKDAIEPWLDGTFTANGFLYDSKWGGIVTKQGSADSGADFGFGVYNDH 470

Query: 466 HFHLGYFVYSIAVLAKLDPNWGKQFKPQAYALVYDYMNFRPKKSQFSIPFRNFDFWKLHS 525
           H+HLGYF+Y+IAVLAK+DP WG+++KPQAY+L+ D+MN   + +      R FD +KLHS
Sbjct: 471 HYHLGYFLYAIAVLAKIDPAWGRKYKPQAYSLMADFMNLGRRSNSNYPRLRCFDLYKLHS 530

Query: 526 WAAGLTEFPDGRNQESTSEAVNAYYAAALMGLAYDDESLTAAGSTLTAAEIAATQTWWHV 585
           WA GLTEF DGRNQESTSEAVNAYY+AALMGLAY D  L   GS L A EI + QTWWHV
Sbjct: 531 WAGGLTEFGDGRNQESTSEAVNAYYSAALMGLAYGDTHLVTMGSMLAAMEILSAQTWWHV 590

Query: 586 KSEKNWIYDKGFEEENRVVGILWSAARESRLWFAPAEWRECRVGIQVLPVLPVSERVFSD 645
           +   N +Y + F  ENRVVG+LW+  R+S LWFAP +W+ECR+GIQV+P+LP++E +FSD
Sbjct: 591 REGDN-LYAEDFTRENRVVGVLWANKRDSGLWFAPPDWKECRLGIQVIPLLPITEVLFSD 650

Query: 646 KGFVKEVVEWVSRALEREDAGEGWKGFAYALEGIYDKKSAIEKVKKLKKHDDGNSLSNLL 705
            GF KE+V+W   AL RE  GEGWKGF Y+LEGIYDK+ A+EKV+ L   DDGNSL+NLL
Sbjct: 651 VGFAKELVKWTLPALAREGVGEGWKGFVYSLEGIYDKEGALEKVRNLNGFDDGNSLTNLL 710

Query: 706 WWIYSRPE 709
           WWI+SR +
Sbjct: 711 WWIHSRSD 713

BLAST of Bhi06G000138 vs. TrEMBL
Match: tr|A0A061EHI7|A0A061EHI7_THECC (Glycosyl hydrolase family 81 protein OS=Theobroma cacao OX=3641 GN=TCM_019610 PE=4 SV=1)

HSP 1 Score: 794.7 bits (2051), Expect = 1.8e-226
Identity = 396/660 (60.00%), Postives = 495/660 (75.00%), Query Frame = 0

Query: 52  FLFPQTTSTAVPDPAKFFSPTLLSSPLPTNSFFQNFVLNNGDKPEYIHPYIVRTANSSLS 111
           FLFP   S+ +PDP+ FFSP LLS+PLPTNSFFQNF L NGD+PEYIHPY++++A+SSLS
Sbjct: 51  FLFPGVQSSVLPDPSVFFSPKLLSTPLPTNSFFQNFTLKNGDQPEYIHPYLIKSADSSLS 110

Query: 112 VSYPSRISNSSITQLQFYPDLTISALDRTSPVSNTTHFISSFSDLGVDLDI--GDFRFHL 171
           +SYPS   NSS     F  DLTI A DRT+P S   H ISS+SDL + LD    + RF+L
Sbjct: 111 ISYPSHFHNSSFLYQIFISDLTIFATDRTNPTSQKNHIISSYSDLSLTLDFPCSNLRFYL 170

Query: 172 VRGSPYLTFSVLKISSVSISTGNGVRSVDSYDDSTKHIIRLNNGRNWVLYSSSAIYLIKS 231
           VRGSP+LT  V   + +SIST + + S  S    TKH I+LNN + W++Y+SSAI L  S
Sbjct: 171 VRGSPFLTCFVTGNTPISISTIHAILSFSSNTSLTKHTIKLNNNQTWLIYTSSAISLNHS 230

Query: 232 KNNQIVTSGGFSGVIRIAVLPNSAMESGNILDRYSGCYPVSGYVKLSENFGFKYKWQKKG 291
            +  ++TSGGFSG+IRIAVLP+S     +ILD+YS CYP+SG   L++ F  +YKW+KKG
Sbjct: 231 LS--LITSGGFSGIIRIAVLPDSDPTYESILDQYSSCYPISGDAVLTKPFCLEYKWEKKG 290

Query: 292 SGGLLMLAHPLHRQILSRNQ---TVLPNLRYGSIDGDLLGVVGDSWDLNFNPVPITWHSI 351
            G LLMLAHPLH  ILSR+    TVL N +Y SIDG+L+G+VGDSW L  + V +TWHSI
Sbjct: 291 WGDLLMLAHPLHLNILSRDDSEVTVLENFKYKSIDGELVGIVGDSWVLKPHQVSVTWHSI 350

Query: 352 NGIDKKFFPEIVAALKRDVATLNVTELSSTASSYFYAKLLARAARLALIAEEVDNGAGVI 411
            G+ ++ + EIV AL +DV  L+ + +++T SSYFY KL+ARAARLALIAEEV     VI
Sbjct: 351 KGVKEESYHEIVTALSKDVEALDSSAITTT-SSYFYGKLIARAARLALIAEEV-CFLDVI 410

Query: 412 PAVVRFLKNGIQPWLIGKFPKNGFLYERKWAGLVTKNGATSTTEDFGFGIYNDHHFHLGY 471
           PA+ +FLK+ I+PWL G F  NGFLY+ KW G+VTK G+T +  DFGFGIYNDHH+H+GY
Sbjct: 411 PAIRKFLKDTIEPWLDGTFNGNGFLYDSKWGGIVTKQGSTDSGADFGFGIYNDHHYHIGY 470

Query: 472 FVYSIAVLAKLDPNWGKQFKPQAYALVYDYMNFRPKKSQFSIPFRNFDFWKLHSWAAGLT 531
           F+Y+IAVLAK+DP WG+++KPQAY LV D+MN   + +      R FD +KLHSWA GLT
Sbjct: 471 FLYAIAVLAKIDPAWGRRYKPQAYTLVADFMNLGRRSNSNYPRLRCFDLYKLHSWAGGLT 530

Query: 532 EFPDGRNQESTSEAVNAYYAAALMGLAYDDESLTAAGSTLTAAEIAATQTWWHVKSEKNW 591
           EF DGRNQESTSEAVNAYY+AALMGLAY D  L A GSTL A EI A QTWWHVK   N 
Sbjct: 531 EFADGRNQESTSEAVNAYYSAALMGLAYGDTHLVATGSTLAAMEIQAAQTWWHVKEGDN- 590

Query: 592 IYDKGFEEENRVVGILWSAARESRLWFAPAEWRECRVGIQVLPVLPVSERVFSDKGFVKE 651
           +Y++ F  ENRVVG+LW+  R+S LWFAPA+WRECR+GIQ+LP+LP+SE +FSD  F +E
Sbjct: 591 LYEEEFTTENRVVGVLWANKRDSGLWFAPADWRECRLGIQLLPILPISEVLFSDISFTRE 650

Query: 652 VVEWVSRALEREDAGEGWKGFAYALEGIYDKKSAIEKVKKLKKHDDGNSLSNLLWWIYSR 707
           +V W   AL RE  GEGWKGF YALE IYDK+SA+EK++ L  +DDGNSL+NLLWW++SR
Sbjct: 651 LVNWTLPALGREGVGEGWKGFVYALEAIYDKESALEKIRNLNGYDDGNSLTNLLWWVHSR 705

BLAST of Bhi06G000138 vs. NCBI nr
Match: XP_011656051.1 (PREDICTED: putative endo-1,3(4)-beta-glucanase 2 [Cucumis sativus] >KGN52617.1 hypothetical protein Csa_5G647400 [Cucumis sativus])

HSP 1 Score: 1213.0 bits (3137), Expect = 0.0e+00
Identity = 600/687 (87.34%), Postives = 629/687 (91.56%), Query Frame = 0

Query: 24  ETPKPWLISIFXXXXXXXTAFPPAVSGQFLFPQTTSTAVPDPAKFFSPTLLSSPLPTNSF 83
           ET KP  IS          A   AVS QF FP+TTSTAVPDP KFFSP LLSSPLPTNSF
Sbjct: 3   ETSKPSPISTLFFLLLLTAA---AVSAQFPFPETTSTAVPDPGKFFSPNLLSSPLPTNSF 62

Query: 84  FQNFVLNNGDKPEYIHPYIVRTANSSLSVSYPSRISNSSITQLQFYPDLTISALDRTSPV 143
           FQNFVLNNGD PEYIHPY++RTANSSLSVSYPSRISNSSITQLQF+PDL IS+  +T   
Sbjct: 63  FQNFVLNNGDLPEYIHPYLIRTANSSLSVSYPSRISNSSITQLQFFPDLVISSPKKT--- 122

Query: 144 SNTTHFISSFSDLGVDLDIGDFRFHLVRGSPYLTFSVLKISSVSISTGNGVRSVDSYDDS 203
            NTTHFISSFSDLGVDLDIG FRFHLVRGSPYLTFSVLK SSV IST NGVRSVDSY+D 
Sbjct: 123 FNTTHFISSFSDLGVDLDIGVFRFHLVRGSPYLTFSVLKTSSVLISTSNGVRSVDSYEDY 182

Query: 204 TKHIIRLNNGRNWVLYSSSAIYLIKSKNNQIVTSGGFSGVIRIAVLPNSAMESGNILDRY 263
           TKHIIRLNNGR+WVLYSSSAIYL+KSK+NQIVTSGGF GVIR+AVLP+SA+ES  ILDRY
Sbjct: 183 TKHIIRLNNGRSWVLYSSSAIYLVKSKSNQIVTSGGFIGVIRVAVLPDSAVESEKILDRY 242

Query: 264 SGCYPVSGYVKLSENFGFKYKWQKKGSGGLLMLAHPLHRQILSRNQTVLPNLRYGSIDGD 323
           SGCYPVSG+VKL   FGF+YKWQKKGSGGLLMLAH LHR+IL R+QTVL N+RY SIDGD
Sbjct: 243 SGCYPVSGFVKLLGGFGFEYKWQKKGSGGLLMLAHTLHREILPRDQTVLQNIRYSSIDGD 302

Query: 324 LLGVVGDSWDLNFNPVPITWHSINGIDKKFFPEIVAALKRDVATLNVTELSSTASSYFYA 383
           LLGVVGDSWDL FNP+PITWHSINGID KFFPEIVAALKRDVATLN TELSS  +SYFY 
Sbjct: 303 LLGVVGDSWDLKFNPIPITWHSINGIDSKFFPEIVAALKRDVATLNATELSSMPASYFYG 362

Query: 384 KLLARAARLALIAEEVDNGAGVIPAVVRFLKNGIQPWLIGKFPKNGFLYERKWAGLVTKN 443
           KLLARAARLALIAEEV+ GAGVIPAVV+FLKNGIQPWLIGKFPKNGFLYERKWAGLVTKN
Sbjct: 363 KLLARAARLALIAEEVNGGAGVIPAVVKFLKNGIQPWLIGKFPKNGFLYERKWAGLVTKN 422

Query: 444 GATSTTEDFGFGIYNDHHFHLGYFVYSIAVLAKLDPNWGKQFKPQAYALVYDYMNFRPKK 503
           GATSTTEDFGFGIYNDHHFHLGYFVYSIAVLAKLDPNWGKQ+KPQAYAL+YDYMNFRPKK
Sbjct: 423 GATSTTEDFGFGIYNDHHFHLGYFVYSIAVLAKLDPNWGKQYKPQAYALLYDYMNFRPKK 482

Query: 504 SQFSIPFRNFDFWKLHSWAAGLTEFPDGRNQESTSEAVNAYYAAALMGLAYDDESLTAAG 563
           SQFSIPFRNFDFWKLHSWAAGLTEFPDGRNQEST+EAVNAYYAAALMGLAYDDESLTAAG
Sbjct: 483 SQFSIPFRNFDFWKLHSWAAGLTEFPDGRNQESTTEAVNAYYAAALMGLAYDDESLTAAG 542

Query: 564 STLTAAEIAATQTWWHVKSEKNWIYDKGFEEENRVVGILWSAARESRLWFAPAEWRECRV 623
           STLTAAEI ATQTWWHVK E N IYDKGF EENR+VGILWSAARESRLWFAPAEWRECRV
Sbjct: 543 STLTAAEITATQTWWHVKRENNGIYDKGFTEENRMVGILWSAARESRLWFAPAEWRECRV 602

Query: 624 GIQVLPVLPVSERVFSDKGFVKEVVEWVSRALEREDAGEGWKGFAYALEGIYDKKSAIEK 683
           GIQVLPVLPV+ERVF+D GFVKEVVEWV  ALEREDAGEGWKGFAYALEGIYDKKSA+EK
Sbjct: 603 GIQVLPVLPVTERVFTDVGFVKEVVEWVQPALEREDAGEGWKGFAYALEGIYDKKSAVEK 662

Query: 684 VKKLKKHDDGNSLSNLLWWIYSRPEGR 711
           VKKLKKHDDGNSLSNLLWWIYSRPEGR
Sbjct: 663 VKKLKKHDDGNSLSNLLWWIYSRPEGR 683

BLAST of Bhi06G000138 vs. NCBI nr
Match: NP_001315392.1 (putative endo-1,3(4)-beta-glucanase 2 precursor [Cucumis melo] >AMP41497.1 beta-1,3-glucanase 2 [Cucumis melo] >ANB86004.1 beta-1,3-glucanase 2 [Cucumis melo] >ANB86008.1 beta-1,3-glucanase 2 [Cucumis melo] >AXM43892.1 beta-1,3-glucanase A [Cucumis melo] >AXM43893.1 beta-1,3-glucanase A [Cucumis melo] >AXM43894.1 beta-1,3-glucanase A [Cucumis melo])

HSP 1 Score: 1192.9 bits (3085), Expect = 0.0e+00
Identity = 586/670 (87.46%), Postives = 621/670 (92.69%), Query Frame = 0

Query: 42  TAFPPAVSGQFLFPQTTSTAVPDPAKFFSPTLLSSPLPTNSFFQNFVLNNGDKPEYIHPY 101
           TA P  VS QF FP+TTSTAVPDP+KFFSP LLSSPLPTNSFFQNFVLNNGD PEYIHPY
Sbjct: 20  TAAP--VSAQFPFPETTSTAVPDPSKFFSPNLLSSPLPTNSFFQNFVLNNGDSPEYIHPY 79

Query: 102 IVRTANSSLSVSYPSRISNSSITQLQFYPDLTISALDRTSPVSNTTHFISSFSDLGVDLD 161
           ++RTANSSL VSYPSRISNSSI QLQF+PDL IS+L++T   SN THF+SSFSDLGVDLD
Sbjct: 80  LIRTANSSLFVSYPSRISNSSINQLQFFPDLVISSLNKT---SNATHFVSSFSDLGVDLD 139

Query: 162 IGDFRFHLVRGSPYLTFSVLKISSVSISTGNGVRSVDSYDDSTKHIIRLNNGRNWVLYSS 221
           IG FR+HLVRGSPYLTFSVLK S V IST NGVRSVDSY+D TK+IIRLNNGR+WVLYSS
Sbjct: 140 IGVFRYHLVRGSPYLTFSVLKTSFVLISTSNGVRSVDSYEDYTKYIIRLNNGRSWVLYSS 199

Query: 222 SAIYLIKSKNNQIVTSGGFSGVIRIAVLPNSAMESGNILDRYSGCYPVSGYVKLSENFGF 281
           SAIYLIKSK+NQIVTSGGF GVIR+AVLP+SA+ES  ILDRYSGCYPVSG+ KLS  FGF
Sbjct: 200 SAIYLIKSKSNQIVTSGGFIGVIRVAVLPDSAVESEKILDRYSGCYPVSGFAKLSGGFGF 259

Query: 282 KYKWQKKGSGGLLMLAHPLHRQILSRNQTVLPNLRYGSIDGDLLGVVGDSWDLNFNPVPI 341
           +YKWQKKGSGGLLMLAH LHR+IL R+QTVL NLRY SIDGDLLGVVGDSWDL FNP+PI
Sbjct: 260 EYKWQKKGSGGLLMLAHTLHREILPRDQTVLQNLRYSSIDGDLLGVVGDSWDLKFNPIPI 319

Query: 342 TWHSINGIDKKFFPEIVAALKRDVATLNVTELSSTASSYFYAKLLARAARLALIAEEVDN 401
           TWHSINGI+ KFFPEIVAALKRDVATLNVTELSST +SYFY KLLARAARLALIAEEV+ 
Sbjct: 320 TWHSINGINSKFFPEIVAALKRDVATLNVTELSSTPASYFYGKLLARAARLALIAEEVNY 379

Query: 402 GAGVIPAVVRFLKNGIQPWLIGKFPKNGFLYERKWAGLVTKNGATSTTEDFGFGIYNDHH 461
           GA VIPAVV+FLKNGIQPWLIGKFPKNGFLYERKWAGLVTKNGATST EDFGFGIYNDHH
Sbjct: 380 GASVIPAVVKFLKNGIQPWLIGKFPKNGFLYERKWAGLVTKNGATSTIEDFGFGIYNDHH 439

Query: 462 FHLGYFVYSIAVLAKLDPNWGKQFKPQAYALVYDYMNFRPKKSQFSIPFRNFDFWKLHSW 521
           FHLGYFVYSIAVLAKLDPNW KQ+KPQAYAL+YDYMNFRPKKSQFSIPFRNFDFWKLHSW
Sbjct: 440 FHLGYFVYSIAVLAKLDPNWRKQYKPQAYALLYDYMNFRPKKSQFSIPFRNFDFWKLHSW 499

Query: 522 AAGLTEFPDGRNQESTSEAVNAYYAAALMGLAYDDESLTAAGSTLTAAEIAATQTWWHVK 581
           AAGL EFPDGRNQESTSEAVNAYYAAALMGLAY D SLTAAGSTLTAAEI A+QTWWHVK
Sbjct: 500 AAGLAEFPDGRNQESTSEAVNAYYAAALMGLAYGDASLTAAGSTLTAAEITASQTWWHVK 559

Query: 582 SEKNWIYDKGFEEENRVVGILWSAARESRLWFAPAEWRECRVGIQVLPVLPVSERVFSDK 641
            EKN IYDKGF EENR+VGILWSAARESRLWFAPAEWRECR+GIQVLPVLPV+ER+F+D 
Sbjct: 560 KEKNGIYDKGFAEENRMVGILWSAARESRLWFAPAEWRECRLGIQVLPVLPVTERMFTDV 619

Query: 642 GFVKEVVEWVSRALEREDAGEGWKGFAYALEGIYDKKSAIEKVKKLKKHDDGNSLSNLLW 701
           GFVKE VEWV  ALEREDAGEGWKGFAYALEGIYDKKSA+EKVKKLKKHDDGNSLSNLLW
Sbjct: 620 GFVKEAVEWVQPALEREDAGEGWKGFAYALEGIYDKKSAVEKVKKLKKHDDGNSLSNLLW 679

Query: 702 WIYSRPEGRG 712
           WIYSRPEGRG
Sbjct: 680 WIYSRPEGRG 684

BLAST of Bhi06G000138 vs. NCBI nr
Match: XP_023534896.1 (probable endo-1,3(4)-beta-glucanase ARB_01444 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1148.3 bits (2969), Expect = 0.0e+00
Identity = 567/693 (81.82%), Postives = 616/693 (88.89%), Query Frame = 0

Query: 23  METPKPWLISIFXXXXXXXTA--FPPAVSGQFLFPQTTSTAVPDPAKFFSPTLLSSPLPT 82
           ME P PWL+  F        +   PP+ S QF FP+TTSTAVPDP+KFFSP+LLSSPLPT
Sbjct: 1   MEAPNPWLLPFFLLLLAAAFSDQLPPS-SAQFPFPETTSTAVPDPSKFFSPSLLSSPLPT 60

Query: 83  NSFFQNFVLNNGDKPEYIHPYIVRTANSSLSVSYPSRISNSSITQLQFYPDLTISALDRT 142
           NSFFQNFVLNNGD PEYIHPY++RTANSSLSVSYPSR SNSSITQL F PDLTIS+    
Sbjct: 61  NSFFQNFVLNNGDLPEYIHPYLIRTANSSLSVSYPSRTSNSSITQLPFSPDLTISS---- 120

Query: 143 SPVSNTTHFISSFSDLGVDLDIGDFRFHLVRGSPYLTFSVLKISSVSIST-GNGVRSVDS 202
             VSN THF+SSFSDL VDLDIGDFRFHLVRGSPYLTFSVLK SSVSIST GNGV SVDS
Sbjct: 121 --VSNKTHFVSSFSDLAVDLDIGDFRFHLVRGSPYLTFSVLKTSSVSISTGGNGVGSVDS 180

Query: 203 YDDSTKHIIRLNNGRNWVLYSSSAIYLIKSKNNQIVTSGGFSGVIRIAVLPNSAMESGNI 262
           YDDSTKHIIRL+NGR WV+YS++AIYL+K K+++IVTSGGF GVIR+AVLPNSA E+  I
Sbjct: 181 YDDSTKHIIRLSNGRTWVVYSTAAIYLMKGKSSEIVTSGGFFGVIRVAVLPNSAAETEQI 240

Query: 263 LDRYSGCYPVSGYVKLSENFGFKYKWQKKGSGGLLMLAHPLHRQILSRNQTVLPNLRYGS 322
           LDRYSGCYPVSGY KLS +FGF YKWQKKGSGGLLMLAHPLHR++LS N TVL N +YGS
Sbjct: 241 LDRYSGCYPVSGYAKLSGSFGFSYKWQKKGSGGLLMLAHPLHRRVLSENLTVLQNFKYGS 300

Query: 323 IDGDLLGVVGDSWDLNFNPVPITWHSINGIDKKFFPEIVAALKRDVATLNVTELSSTASS 382
           IDGDL+GVVGDSWDLNF P+PITWHSINGI++KFFPEIVAALK DV TLNVTELSSTA+S
Sbjct: 301 IDGDLVGVVGDSWDLNFAPIPITWHSINGIERKFFPEIVAALKHDVGTLNVTELSSTAAS 360

Query: 383 YFYAKLLARAARLALIAEEVDNGAGVIPAVVRFLKNGIQPWLIGKFPKNGFLYERKWAGL 442
           YFY KLLARAARLALIAEEVD  AGVIPAVV+FLK G+QPWL GKF +NGFLY+RKWAGL
Sbjct: 361 YFYGKLLARAARLALIAEEVDYAAGVIPAVVKFLKTGVQPWLDGKFGRNGFLYDRKWAGL 420

Query: 443 VTKNGATSTTEDFGFGIYNDHHFHLGYFVYSIAVLAKLDPNWGKQFKPQAYALVYDYMNF 502
           VTKNGATS TEDFGFGIYNDHHFHLGYFVYSIAVLAKLD NWG Q+K  AYALVYDYMN+
Sbjct: 421 VTKNGATSKTEDFGFGIYNDHHFHLGYFVYSIAVLAKLDRNWGSQYKAPAYALVYDYMNY 480

Query: 503 RPKKSQFSIPFRNFDFWKLHSWAAGLTEFPDGRNQESTSEAVNAYYAAALMGLAYDDESL 562
           R K +QFSIPFRNFDFWKLHSWAAGLTEFPDGRNQESTSEA+NAYYAAALMGLAY DESL
Sbjct: 481 RSKNTQFSIPFRNFDFWKLHSWAAGLTEFPDGRNQESTSEAINAYYAAALMGLAYGDESL 540

Query: 563 TAAGSTLTAAEIAATQTWWHV-KSEKNWIYDKGFEEENRVVGILWSAARESRLWFAPAEW 622
           TA GS LTAAEIAA QTWWHV +  ++ IYD+GF EENRVVGILWS ARESRLWFA AEW
Sbjct: 541 TAVGSALTAAEIAAAQTWWHVNRGGRSSIYDEGFTEENRVVGILWSGARESRLWFATAEW 600

Query: 623 RECRVGIQVLPVLPVSERVFSDKGFVKEVVEWVSRALEREDAGEGWKGFAYALEGIYDKK 682
           RECRVGIQVLPV+PV+ERVF+D+GFVKEVVEWVS ALEREDAGEGWKGF YALEGIYDK+
Sbjct: 601 RECRVGIQVLPVVPVTERVFADEGFVKEVVEWVSPALEREDAGEGWKGFVYALEGIYDKE 660

Query: 683 SAIEKVKKLKKHDDGNSLSNLLWWIYSRPEGRG 712
           +A+ KVKKLKKHDDGNSLSNLLWWIYSRPE +G
Sbjct: 661 NAVGKVKKLKKHDDGNSLSNLLWWIYSRPERQG 686

BLAST of Bhi06G000138 vs. NCBI nr
Match: XP_022925518.1 (probable endo-1,3(4)-beta-glucanase ARB_01444 [Cucurbita moschata])

HSP 1 Score: 1145.6 bits (2962), Expect = 0.0e+00
Identity = 569/695 (81.87%), Postives = 615/695 (88.49%), Query Frame = 0

Query: 23  METPKPWLISIFXXXXXXXTAFP---PAVSGQFLFPQTTSTAVPDPAKFFSPTLLSSPLP 82
           ME P PWL+  F        AF    P  S QF FP+TTSTAVPDP KFFSP+LLSSPLP
Sbjct: 1   MEAPNPWLLPFF-LLLLLAAAFSDQLPPSSAQFPFPETTSTAVPDPTKFFSPSLLSSPLP 60

Query: 83  TNSFFQNFVLNNGDKPEYIHPYIVRTANSSLSVSYPSRISNSSITQLQFYPDLTISALDR 142
           TNSFFQNFVLNNGD PEYIHPY++RTANSSLSVSYPSR SNSSITQL F PDLTIS+   
Sbjct: 61  TNSFFQNFVLNNGDLPEYIHPYLIRTANSSLSVSYPSRTSNSSITQLPFSPDLTISS--- 120

Query: 143 TSPVSNTTHFISSFSDLGVDLDIGDFRFHLVRGSPYLTFSVLKISSVSIST-GNGVRSVD 202
              VSN THF+SSFSDL VDLDIGDFRFHLVRGSPYLTFSVLK SS+SIST GNGV SVD
Sbjct: 121 ---VSNKTHFVSSFSDLAVDLDIGDFRFHLVRGSPYLTFSVLKTSSISISTGGNGVGSVD 180

Query: 203 SYDDSTKHIIRLNNGRNWVLYSSSAIYLIKSKNNQIVTSGGFSGVIRIAVLPNSAMESGN 262
           SYD STKHIIRL+NGR+WV+YS++AIYL+K K+++IVTSGGFSGVIR+AVLPNSA E+  
Sbjct: 181 SYDGSTKHIIRLSNGRSWVVYSTAAIYLMKGKSSEIVTSGGFSGVIRVAVLPNSAAETEQ 240

Query: 263 ILDRYSGCYPVSGYVKLSENFGFKYKWQKKGSGGLLMLAHPLHRQILSRNQTVLPNLRYG 322
           ILDRYSGCYPVSGY KLS NFGF YKWQKKGSGGLLMLAHPLHR++LS N TVL N +YG
Sbjct: 241 ILDRYSGCYPVSGYAKLSGNFGFSYKWQKKGSGGLLMLAHPLHRRVLSENLTVLQNFKYG 300

Query: 323 SIDGDLLGVVGDSWDLNFNPVPITWHSINGIDKKFFPEIVAALKRDVATLNVTELSSTAS 382
           SIDGDL+GVVGDSWDLNF P+PITWHSINGI++KFFPEIVAALK DV TLNVTELSSTA+
Sbjct: 301 SIDGDLVGVVGDSWDLNFAPIPITWHSINGIERKFFPEIVAALKHDVGTLNVTELSSTAA 360

Query: 383 SYFYAKLLARAARLALIAEEVDNGAGVIPAVVRFLKNGIQPWLIGKFPKNGFLYERKWAG 442
           SYFY KLLARAARLALIAEEVD  AGVIPAVV+FLK G+QPWL GKF +NGFLY+RKWAG
Sbjct: 361 SYFYGKLLARAARLALIAEEVDYAAGVIPAVVKFLKTGVQPWLDGKFGRNGFLYDRKWAG 420

Query: 443 LVTKNGATSTTEDFGFGIYNDHHFHLGYFVYSIAVLAKLDPNWGKQFKPQAYALVYDYMN 502
           LVTKNGATS TEDFGFGIYNDHHFHLGYFVYSIAVLAKLD NWG Q+K  AYALVYDYMN
Sbjct: 421 LVTKNGATSKTEDFGFGIYNDHHFHLGYFVYSIAVLAKLDRNWGNQYKAPAYALVYDYMN 480

Query: 503 FRPKKSQFSIPFRNFDFWKLHSWAAGLTEFPDGRNQESTSEAVNAYYAAALMGLAYDDES 562
           +R K SQFSIPFRNFDFWKLHSWAAGLTEFPDGRNQESTSEA+NAYYAAALMGLAY DES
Sbjct: 481 YRSKNSQFSIPFRNFDFWKLHSWAAGLTEFPDGRNQESTSEAINAYYAAALMGLAYGDES 540

Query: 563 LTAAGSTLTAAEIAATQTWWHV-KSEKNWIYDKGFEEENRVVGILWSAARESRLWFAPAE 622
           LTA GS LTAAEIAA QTWWHV +  ++ IYD+GF EENRVVGILWS ARESRLWFA AE
Sbjct: 541 LTAVGSALTAAEIAAAQTWWHVNRGGRSSIYDEGFTEENRVVGILWSGARESRLWFATAE 600

Query: 623 WRECRVGIQVLPVLPVSERVFSDKGFVKEVVEWVSRALEREDAGEGWKGFAYALEGIYD- 682
           WRECRVGIQVLPV+PV+ERVF+D+GFVKEVVEWVS ALEREDAGEGWKGF YALEGIYD 
Sbjct: 601 WRECRVGIQVLPVVPVTERVFADEGFVKEVVEWVSPALEREDAGEGWKGFVYALEGIYDN 660

Query: 683 KKSAIEKVKKLKKHDDGNSLSNLLWWIYSRPEGRG 712
           K++A+ KVKKLKKHDDGNSLSNLLWWIYSRPE +G
Sbjct: 661 KENAVGKVKKLKKHDDGNSLSNLLWWIYSRPERQG 688

BLAST of Bhi06G000138 vs. NCBI nr
Match: XP_022973486.1 (putative endo-1,3(4)-beta-glucanase 2 [Cucurbita maxima])

HSP 1 Score: 1141.7 bits (2952), Expect = 0.0e+00
Identity = 562/691 (81.33%), Postives = 611/691 (88.42%), Query Frame = 0

Query: 23  METPKPWLISIFXXXXXXXTAFPPAVSGQFLFPQTTSTAVPDPAKFFSPTLLSSPLPTNS 82
           M    PWL+  F       +   P  S QF FP+TTSTAVPDP KFFSP+LLSSPLPTNS
Sbjct: 1   MAATNPWLLPFFLLLAAAYSDQLPPSSAQFPFPETTSTAVPDPTKFFSPSLLSSPLPTNS 60

Query: 83  FFQNFVLNNGDKPEYIHPYIVRTANSSLSVSYPSRISNSSITQLQFYPDLTISALDRTSP 142
           FFQNFVLN GD PEYIHPY++RTANSSLSVSYPSR SN+SITQL F PDLTIS+      
Sbjct: 61  FFQNFVLNKGDLPEYIHPYLIRTANSSLSVSYPSRTSNTSITQLPFSPDLTISS------ 120

Query: 143 VSNTTHFISSFSDLGVDLDIGDFRFHLVRGSPYLTFSVLKISSVSIST-GNGVRSVDSYD 202
           VSN THF+SSFSDL VDLDIG+FRFHLVRGSPYLTFSVLK SSVSIST GNGV SVDSYD
Sbjct: 121 VSNKTHFVSSFSDLAVDLDIGEFRFHLVRGSPYLTFSVLKTSSVSISTGGNGVDSVDSYD 180

Query: 203 DSTKHIIRLNNGRNWVLYSSSAIYLIKSKNNQIVTSGGFSGVIRIAVLPNSAMESGNILD 262
            STKHIIRL+NGRNWV+YS++AIYL+K+K+++IVTSGGFSGVIR+AVLPNSA E+  ILD
Sbjct: 181 GSTKHIIRLSNGRNWVVYSTAAIYLMKAKSSEIVTSGGFSGVIRVAVLPNSAAETEQILD 240

Query: 263 RYSGCYPVSGYVKLSENFGFKYKWQKKGSGGLLMLAHPLHRQILSRNQTVLPNLRYGSID 322
           RYSGCYPVSGY KLS NFGF YKWQKKGSGGLLMLAHPLHR++L  N TVL N +YGSID
Sbjct: 241 RYSGCYPVSGYAKLSGNFGFSYKWQKKGSGGLLMLAHPLHRRVLPENLTVLQNFKYGSID 300

Query: 323 GDLLGVVGDSWDLNFNPVPITWHSINGIDKKFFPEIVAALKRDVATLNVTELSSTASSYF 382
           GDL+GVVGDSWDLNF P+PITWHSINGI++KFFPEIVAALK DV TLNVTELSSTA+SYF
Sbjct: 301 GDLVGVVGDSWDLNFAPIPITWHSINGIERKFFPEIVAALKHDVGTLNVTELSSTAASYF 360

Query: 383 YAKLLARAARLALIAEEVDNGAGVIPAVVRFLKNGIQPWLIGKFPKNGFLYERKWAGLVT 442
           Y KLLARAARLALIAEEVD  AGVIPAVV+FLK GIQPWL GKF +NGFLY+RKWAGLVT
Sbjct: 361 YGKLLARAARLALIAEEVDYAAGVIPAVVKFLKTGIQPWLDGKFGRNGFLYDRKWAGLVT 420

Query: 443 KNGATSTTEDFGFGIYNDHHFHLGYFVYSIAVLAKLDPNWGKQFKPQAYALVYDYMNFRP 502
           KNGATS TEDFGFGIYNDHHFHLGYFVYSIAVLAKLD NWG Q+K  AYALV+DYMN+R 
Sbjct: 421 KNGATSKTEDFGFGIYNDHHFHLGYFVYSIAVLAKLDRNWGNQYKAPAYALVHDYMNYRS 480

Query: 503 KKSQFSIPFRNFDFWKLHSWAAGLTEFPDGRNQESTSEAVNAYYAAALMGLAYDDESLTA 562
           + +QFSIPFRNFDFWKLHSWAAGLTEFPDGRNQESTSEA+NAYYAAALMGLAY DESLTA
Sbjct: 481 RNTQFSIPFRNFDFWKLHSWAAGLTEFPDGRNQESTSEAINAYYAAALMGLAYGDESLTA 540

Query: 563 AGSTLTAAEIAATQTWWHV-KSEKNWIYDKGFEEENRVVGILWSAARESRLWFAPAEWRE 622
            GS LTAAEIAA QTWWHV +  ++ IYD+GF EENRVVGILWS ARESRLWFA AEWRE
Sbjct: 541 VGSALTAAEIAAAQTWWHVNRGGRSSIYDEGFTEENRVVGILWSGARESRLWFASAEWRE 600

Query: 623 CRVGIQVLPVLPVSERVFSDKGFVKEVVEWVSRALEREDAGEGWKGFAYALEGIYDKKSA 682
           CRVGIQVLPV+PV+ERVF+D+GFVKEVVEWVS ALEREDAGEGWKGF YALEGIYDKK+A
Sbjct: 601 CRVGIQVLPVVPVTERVFADEGFVKEVVEWVSPALEREDAGEGWKGFVYALEGIYDKKNA 660

Query: 683 IEKVKKLKKHDDGNSLSNLLWWIYSRPEGRG 712
           + KVKKLKKHDDGNSLSNLLWWIYSRPE +G
Sbjct: 661 VGKVKKLKKHDDGNSLSNLLWWIYSRPERQG 685

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
sp|D4AZ24|ENG1_ARTBC1.2e-4127.09Probable endo-1,3(4)-beta-glucanase ARB_01444 OS=Arthroderma benhamiae (strain A... [more]
sp|Q09850|ENG2_SCHPO1.0e-4026.03Putative endo-1,3(4)-beta-glucanase 2 OS=Schizosaccharomyces pombe (strain 972 /... [more]
sp|P53753|ENG1_YEAST1.2e-3624.42Endo-1,3(4)-beta-glucanase 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S... [more]
sp|Q12168|ENG2_YEAST4.6e-3625.98Endo-1,3(4)-beta-glucanase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S... [more]
sp|Q9UT45|ENG1_SCHPO7.8e-2824.41Endo-1,3(4)-beta-glucanase 1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 248... [more]
Match NameE-valueIdentityDescription
AT5G15870.16.5e-21157.77glycosyl hydrolase family 81 protein[more]
AT1G18310.11.0e-19551.90glycosyl hydrolase family 81 protein[more]
Match NameE-valueIdentityDescription
tr|A0A0A0KW06|A0A0A0KW06_CUCSA0.0e+0087.34Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G647400 PE=4 SV=1[more]
tr|A0A1S3C9V5|A0A1S3C9V5_CUCME0.0e+0084.31putative endo-1,3(4)-beta-glucanase 2 OS=Cucumis melo OX=3656 GN=LOC103498635 PE... [more]
tr|A0A142BTQ9|A0A142BTQ9_CUCME0.0e+0087.46Beta-1,3-glucanase 2 OS=Cucumis melo OX=3656 GN=glu2 PE=2 SV=1[more]
tr|A0A1Q3DC08|A0A1Q3DC08_CEPFO4.6e-22759.28Glyco_hydro_81 domain-containing protein OS=Cephalotus follicularis OX=3775 GN=C... [more]
tr|A0A061EHI7|A0A061EHI7_THECC1.8e-22660.00Glycosyl hydrolase family 81 protein OS=Theobroma cacao OX=3641 GN=TCM_019610 PE... [more]
Match NameE-valueIdentityDescription
XP_011656051.10.0e+0087.34PREDICTED: putative endo-1,3(4)-beta-glucanase 2 [Cucumis sativus] >KGN52617.1 h... [more]
NP_001315392.10.0e+0087.46putative endo-1,3(4)-beta-glucanase 2 precursor [Cucumis melo] >AMP41497.1 beta-... [more]
XP_023534896.10.0e+0081.82probable endo-1,3(4)-beta-glucanase ARB_01444 [Cucurbita pepo subsp. pepo][more]
XP_022925518.10.0e+0081.87probable endo-1,3(4)-beta-glucanase ARB_01444 [Cucurbita moschata][more]
XP_022973486.10.0e+0081.33putative endo-1,3(4)-beta-glucanase 2 [Cucurbita maxima][more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0052861glucan endo-1,3-beta-glucanase activity, C-3 substituted reducing group
Vocabulary: INTERPRO
TermDefinition
IPR005200Endo-beta-glucanase
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008152 metabolic process
biological_process GO:0016998 cell wall macromolecule catabolic process
biological_process GO:0006076 (1->3)-beta-D-glucan catabolic process
biological_process GO:0005982 starch metabolic process
biological_process GO:0005985 sucrose metabolic process
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
cellular_component GO:0009986 cell surface
molecular_function GO:0030246 carbohydrate binding
molecular_function GO:0008810 cellulase activity
molecular_function GO:0052861 glucan endo-1,3-beta-glucanase activity, C-3 substituted reducing group
molecular_function GO:0052862 glucan endo-1,4-beta-glucanase activity, C-3 substituted reducing group
molecular_function GO:0042973 glucan endo-1,3-beta-D-glucosidase activity
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0016798 hydrolase activity, acting on glycosyl bonds

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi06M000138Bhi06M000138mRNA


Analysis Name: InterPro Annotations of wax gourd
Date Performed: 2019-11-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:2.70.98.30coord: 69..262
e-value: 5.6E-17
score: 64.1
NoneNo IPR availablePANTHERPTHR31983:SF0GLYCOSYL HYDROLASE FAMILY 81 PROTEINcoord: 54..706
IPR005200Endo-1,3(4)-beta-glucanasePFAMPF03639Glyco_hydro_81coord: 63..703
e-value: 2.4E-167
score: 558.5
IPR005200Endo-1,3(4)-beta-glucanasePANTHERPTHR31983FAMILY NOT NAMEDcoord: 54..706