Csa5G154750 (gene) Cucumber (Chinese Long) v2

NameCsa5G154750
Typegene
OrganismCucumis. sativus (Cucumber (Chinese Long) v2)
DescriptionSerine/threonine dehydratase serine racemase; contains IPR001926 (Tryptophan synthase beta subunit-like PLP-dependent enzymes superfamily)
LocationChr5 : 5159807 .. 5163511 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAACGTGGAGAGTCAAAATAAGAAGAAAGAGTATGCTGCTGATATTTCCTCCATAAAGGAAGCCCGGATACGTATTCGACCTTTCATCCATCAAACCCCAGTTTTTTCATCTGAAACTATCAATGCTGCTTCAGGAAAACAACTTTTCTTCAAATGTGAATGTTTCCAGAAGGGGTGAGTAGCTTAACCAACGTTGAAATATTATAAGGTTCTGATTCAAATGGTGTGTGGTCTTTTTAATGCTGACATGCACAGTAACATTTAAACTTATTTTCAGTTTCAACGTAACTGACTCTTGAAATCTATTATGAAATGTGTTATCTTGTTTCTAAGTCAAAAGCCATCTTTTAAAGATAAATGGACAGGTGATGATTCATCTTTTGAAGAAACGACGTTACTTGATATTTTAGATATATTAGTTCTAAATCATCTCTCTTAGATTATTATTCAATAGTTAATAGGTACAATGTAAATTTGATTATTTCAAAGTTTGTATGAAAAGCTCTTCTTGCCATGTTGCTGTTGGTACTTTTGCTAAGAGTACTCTTTGTGAAGTCAAGTCAAGGATAAGTTGAAAATGTCTGTGAGCTATTCTATCATAATTTTGAAATAAATTTGTCATAATATTAAAGGTAGGAGAGAGAATTATGGTTTCTAATGGAAATCTAATGGTTTATTATTTGGCTATTCAGTGGAGCTTTCAAATTCAGAGGTGCCTGCAATGCTATATATTCACTTGATGAGGGCGAGGCTGCTAAAGGGGTTGTGACACATAGCAGGTCCACTCTGGACTACACAATATACTTCTTTGAAGCTTTTTGAGAACATTTAACTGATAATGCTCTCCAAAATTAGTGGCAACCATGCTGCAGCTTTGTCTTTGGCTGCAAAACTGCGAGGAATCCCTGCATATATAGTTATACCAGAAAATGCTCCCAAATGTAAAGTTGAAAATGTGATACGTTATGGTGGTCAGATTATTTGGAGCAAAAGCGCAATACAGTCAAGGGAATCGGTTGCTGCGAGGGTTATGCAAGAAACTGGTGCACTTCTCATACACCCATATAATGATGGGCGCATAATAAGGTAAAATAATGTTAATATTGTTTTGTTTAACTGTCTTTGGAAGTACTCATGATGATTTCTCAAAGTACAAATCTTTATTTACTTTTAATTTTTTTATCCCTATTTTGAAAATGGATACAAAGTTTGTTAGTGAAACAATGAAAGAGACGAGTGCTGAAAGCCAAATGTAGAAACAATTAAAACTTGCCTTGAAACTGAACAAAACAATCAAGTGTCTTTGATAAGTTTTCCCTATTTTATAAAGACAAATATCTTGAATGGAAAGGTCTGCAAATAATTTGGAGAGCAAGTACCAAAAAGAAGCTTTAACCGAGCAATTTTACAAGGTTCAGATCAATTGGTTTAGGTTTGATTCCTTTCAAAACAAATTTTGGAGAGGATGGCTTTAACCGCATTTCACCACACCAACTGACATTGAGGCTTCAAGAAAGGACCCACAAGAATCTAAACGACATTTTCCTTAAAACCATCACAAAATATCCACTGGATCCTGAAAGTTGAAAACAACTTGAGACAAGAAGCCATGGAACAACACCATTCATAGAAAAAATGTTGAGGATCTTCATTGGCCGAGAACAAAGAGGACACATAGAGGATAATGAGGTGTTAGAAGGTAGCTTCTTTTGAAGTACATTAGAACAATTGAGTGCCCCATAAATCATAATCCATGTGAGTAAATAGATTTCTACAGAGCCTTTTGCAACAACTTATCCATAGTAGAAGAGACAGGCAAGTGCTTGAAAATGGAATTAACAGAAAACTGACATTTGAAACTTTAAAAACTCAGAACTTAATCATCCTTTAAAAATCTTTTAAAAGATATTTGACCAGAAACGAGTGATATCATACCAATTATCAAGAATAGAGCCTTTCAGATTTGAAGAAATTCTTGAAAGGCTTGGAAAAATGGAGCCCAAAGAACTACTGTCCAGCCAAGTATCATGCTAAAAGGAAATTATACTGCCGTTTCTGAGCTTTTATCACTATTCTTAACGCATTCTTTTATTTGACTAATTTTAATTTGTTGTTTCGTCACAAGACTAGTTTTCATAACAAGAACTTGGTTTTACTCAACTGAAATGGATATGTTATGGCATGTTGATCGCTCTTGCAGTGGGCAAGGTACCATCTCGTTGGAGCTTCTGGAACAAGTCCCACAATTAGACACTTTAATCGTTCCCATAAGTGGTTTGTTTGATTGGATCGTTTTATACCTTTCAATTTTCATTGTTAATTTTATGCATTATCATAGTTGATAATATCCCATTGGGACAGGCTTGTTCTTTTCTGAAACCCTTCACCATGAAAATGTAGGTGGTGGTTTGATATCAGGAATTTCAGTGGCTGCAAAGGCCATCAATCCTGCCATCCGGATTTTTGCTGCAGAACCTAAGGGAGCTAATGATGCAGCCATGTCAAAAGCAGCTGGTAAGATAGTAACACTGCCAGAAACCACCACCATAGCCGATGGTCTTCGAGCTTTTCTCGGCAACCTGACCTGGTAATATAACTTCTCTCTTATTTAAATTTTCATCAACGTTAGAAATTGTTTGACTCGTGGATTTAATTATAGGGAAATTTAGTCCCTTTCTCTCCTACAAGTGATTACTATTTATCTCTTGCAAAGCATACTGCAAACCCTCTTTAGTTTCATTCAACTACTCTTTGTACTATTTTTCTCTTCTTCTCATTCTTTCATTTATAGTTTGCCTCTTCATATTCTTAATGATTAAGTTTTATCATTAATACTTTGTTCCCACCCATTTGCAAATTTTCTCTTGTTTATATTGTAAACTAATTTTAATCATAAACTTGAGAACCACCGATAGAAACAGAAGCATGTAATTCAGTAGAAATTGAAGTGTACTTCCCTCCCTTGTGGTAGTAAATTAAACTGATGTTTCTTTCTCCTTCAAGTTTATCTACTATACTACAGTACCAACATGAATATGATTTCCACCAATCATACCATGACTTTTCTGTCTATCAATAACAACTTGGACTACTGAGTGAATGCAAAATAGTAGACTACTATCTTAGCAATAATGAACCAACCTAGACTTTGCATTGGATCCATCTGTTAAGTAATCCATACATCACGATAAGTTCAAATGATCATACTCCCACATTTATTGTATTAATCTAACCTTTCTAACCCAAGACTCATAAAAGCGTAGGCCTATGACAGTAATGTTTTCGCTAATGCATCATTTTGTTGGAACTACTAGTATATATAGCATTTATTTTAAATACTGAAATTCTTGTCATATGATATCTCTCAACTTTGCTTGAATGGTTAAAAACTGATCAAGATTCTTTCTGTGGATTTCATTTACTACTAAATGACTTAATTCCTTGTTGAATGATTAGGCCAATAGTAAGAGATTTGGTGGATGATGTAATAACTGTGGAGGACATAGAGATAGTTGAAGCAATGAGACTTTGTCTTGAAATTCTGAAAGTTGTTGTGGAACCAAGTGGGGCAATAGGTCTTGCTGCTGTTCTCTCTGATAGTTTCAAGCAAAACCCTTCATGGAAGGATTGCAACTCCATTGGCATTATACTTTCTGGAGGTAATGTTGATCTTGGCATGCTTTGGAATTCTTACAAAAAATGA

mRNA sequence

ATGAACGTGGAGAGTCAAAATAAGAAGAAAGAGTATGCTGCTGATATTTCCTCCATAAAGGAAGCCCGGATACGTATTCGACCTTTCATCCATCAAACCCCAGTTTTTTCATCTGAAACTATCAATGCTGCTTCAGGAAAACAACTTTTCTTCAAATGTGAATGTTTCCAGAAGGGTGGAGCTTTCAAATTCAGAGGTGCCTGCAATGCTATATATTCACTTGATGAGGGCGAGGCTGCTAAAGGGGTTGTGACACATAGCAGTGGCAACCATGCTGCAGCTTTGTCTTTGGCTGCAAAACTGCGAGGAATCCCTGCATATATAGTTATACCAGAAAATGCTCCCAAATGTAAAGTTGAAAATGTGATACGTTATGGTGGTCAGATTATTTGGAGCAAAAGCGCAATACAGTCAAGGGAATCGGTTGCTGCGAGGGTTATGCAAGAAACTGGTGCACTTCTCATACACCCATATAATGATGGGCGCATAATAAGTGGGCAAGGTACCATCTCGTTGGAGCTTCTGGAACAAGTCCCACAATTAGACACTTTAATCGTTCCCATAAGTGGTGGTGGTTTGATATCAGGAATTTCAGTGGCTGCAAAGGCCATCAATCCTGCCATCCGGATTTTTGCTGCAGAACCTAAGGGAGCTAATGATGCAGCCATGTCAAAAGCAGCTGGTAAGATAGTAACACTGCCAGAAACCACCACCATAGCCGATGGTCTTCGAGCTTTTCTCGGCAACCTGACCTGGCCAATAGTAAGAGATTTGGTGGATGATGTAATAACTGTGGAGGACATAGAGATAGTTGAAGCAATGAGACTTTGTCTTGAAATTCTGAAAGTTGTTGTGGAACCAAGTGGGGCAATAGGTCTTGCTGCTGTTCTCTCTGATAGTTTCAAGCAAAACCCTTCATGGAAGGATTGCAACTCCATTGGCATTATACTTTCTGGAGGTAATGTTGATCTTGGCATGCTTTGGAATTCTTACAAAAAATGA

Coding sequence (CDS)

ATGAACGTGGAGAGTCAAAATAAGAAGAAAGAGTATGCTGCTGATATTTCCTCCATAAAGGAAGCCCGGATACGTATTCGACCTTTCATCCATCAAACCCCAGTTTTTTCATCTGAAACTATCAATGCTGCTTCAGGAAAACAACTTTTCTTCAAATGTGAATGTTTCCAGAAGGGTGGAGCTTTCAAATTCAGAGGTGCCTGCAATGCTATATATTCACTTGATGAGGGCGAGGCTGCTAAAGGGGTTGTGACACATAGCAGTGGCAACCATGCTGCAGCTTTGTCTTTGGCTGCAAAACTGCGAGGAATCCCTGCATATATAGTTATACCAGAAAATGCTCCCAAATGTAAAGTTGAAAATGTGATACGTTATGGTGGTCAGATTATTTGGAGCAAAAGCGCAATACAGTCAAGGGAATCGGTTGCTGCGAGGGTTATGCAAGAAACTGGTGCACTTCTCATACACCCATATAATGATGGGCGCATAATAAGTGGGCAAGGTACCATCTCGTTGGAGCTTCTGGAACAAGTCCCACAATTAGACACTTTAATCGTTCCCATAAGTGGTGGTGGTTTGATATCAGGAATTTCAGTGGCTGCAAAGGCCATCAATCCTGCCATCCGGATTTTTGCTGCAGAACCTAAGGGAGCTAATGATGCAGCCATGTCAAAAGCAGCTGGTAAGATAGTAACACTGCCAGAAACCACCACCATAGCCGATGGTCTTCGAGCTTTTCTCGGCAACCTGACCTGGCCAATAGTAAGAGATTTGGTGGATGATGTAATAACTGTGGAGGACATAGAGATAGTTGAAGCAATGAGACTTTGTCTTGAAATTCTGAAAGTTGTTGTGGAACCAAGTGGGGCAATAGGTCTTGCTGCTGTTCTCTCTGATAGTTTCAAGCAAAACCCTTCATGGAAGGATTGCAACTCCATTGGCATTATACTTTCTGGAGGTAATGTTGATCTTGGCATGCTTTGGAATTCTTACAAAAAATGA

Protein sequence

MNVESQNKKKEYAADISSIKEARIRIRPFIHQTPVFSSETINAASGKQLFFKCECFQKGGAFKFRGACNAIYSLDEGEAAKGVVTHSSGNHAAALSLAAKLRGIPAYIVIPENAPKCKVENVIRYGGQIIWSKSAIQSRESVAARVMQETGALLIHPYNDGRIISGQGTISLELLEQVPQLDTLIVPISGGGLISGISVAAKAINPAIRIFAAEPKGANDAAMSKAAGKIVTLPETTTIADGLRAFLGNLTWPIVRDLVDDVITVEDIEIVEAMRLCLEILKVVVEPSGAIGLAAVLSDSFKQNPSWKDCNSIGIILSGGNVDLGMLWNSYKK*
BLAST of Csa5G154750 vs. Swiss-Prot
Match: SRR_ARATH (Serine racemase OS=Arabidopsis thaliana GN=SR PE=1 SV=1)

HSP 1 Score: 503.4 bits (1295), Expect = 1.9e-141
Identity = 237/324 (73.15%), Postives = 292/324 (90.12%), Query Frame = 1

Query: 9   KKEYAADISSIKEARIRIRPFIHQTPVFSSETINAASGKQLFFKCECFQKGGAFKFRGAC 68
           +++YAADI SIKEA  RI+P+IH+TPV +SE++N+ SG+ LFFKCEC QKGGAFKFRGAC
Sbjct: 5   REKYAADILSIKEAHDRIKPYIHRTPVLTSESLNSISGRSLFFKCECLQKGGAFKFRGAC 64

Query: 69  NAIYSLDEGEAAKGVVTHSSGNHAAALSLAAKLRGIPAYIVIPENAPKCKVENVIRYGGQ 128
           NA+ SLD  +AAKGVVTHSSGNHAAALSLAAK++GIPAYIV+P+ APKCKV+NVIRYGG+
Sbjct: 65  NAVLSLDAEQAAKGVVTHSSGNHAAALSLAAKIQGIPAYIVVPKGAPKCKVDNVIRYGGK 124

Query: 129 IIWSKSAIQSRESVAARVMQETGALLIHPYNDGRIISGQGTISLELLEQVPQLDTLIVPI 188
           +IWS++ + SRE +A++V+QETG++LIHPYNDGRIISGQGTI+LELLEQ+ ++D ++VPI
Sbjct: 125 VIWSEATMSSREEIASKVLQETGSVLIHPYNDGRIISGQGTIALELLEQIQEIDAIVVPI 184

Query: 189 SGGGLISGISVAAKAINPAIRIFAAEPKGANDAAMSKAAGKIVTLPETTTIADGLRAFLG 248
           SGGGLISG+++AAK+I P+IRI AAEPKGA+DAA SK AGKI+TLP T TIADGLRA LG
Sbjct: 185 SGGGLISGVALAAKSIKPSIRIIAAEPKGADDAAQSKVAGKIITLPVTNTIADGLRASLG 244

Query: 249 NLTWPIVRDLVDDVITVEDIEIVEAMRLCLEILKVVVEPSGAIGLAAVLSDSFKQNPSWK 308
           +LTWP+VRDLVDDV+T+E+ EI+EAM++C EILKV VEPSGAIGLAAVLS+SF+ NPS +
Sbjct: 245 DLTWPVVRDLVDDVVTLEECEIIEAMKMCYEILKVSVEPSGAIGLAAVLSNSFRNNPSCR 304

Query: 309 DCNSIGIILSGGNVDLGMLWNSYK 333
           DC +IGI+LSGGNVDLG LW+S+K
Sbjct: 305 DCKNIGIVLSGGNVDLGSLWDSFK 328

BLAST of Csa5G154750 vs. Swiss-Prot
Match: SRR_ORYSJ (Serine racemase OS=Oryza sativa subsp. japonica GN=SERR PE=1 SV=2)

HSP 1 Score: 484.2 bits (1245), Expect = 1.2e-135
Identity = 234/322 (72.67%), Postives = 280/322 (86.96%), Query Frame = 1

Query: 12  YAADISSIKEARIRIRPFIHQTPVFSSETINAASGKQLFFKCECFQKGGAFKFRGACNAI 71
           YAADI SI+EA+ RI P++H+TPV SS +I+A  GKQLFFKCECFQK GAFK RGA N+I
Sbjct: 17  YAADIHSIREAQARIAPYVHKTPVLSSTSIDAIVGKQLFFKCECFQKAGAFKIRGASNSI 76

Query: 72  YSLDEGEAAKGVVTHSSGNHAAALSLAAKLRGIPAYIVIPENAPKCKVENVIRYGGQIIW 131
           ++LD+ EA+KGVVTHSSGNHAAA++LAAKLRGIPAYIVIP NAP CKV+NV RYGG IIW
Sbjct: 77  FALDDDEASKGVVTHSSGNHAAAVALAAKLRGIPAYIVIPRNAPACKVDNVKRYGGHIIW 136

Query: 132 SKSAIQSRESVAARVMQETGALLIHPYNDGRIISGQGTISLELLEQVPQLDTLIVPISGG 191
           S  +I+SRESVA RV +ETGA+L+HP+N+   ISGQGT+SLELLE+VP++DT+IVPISGG
Sbjct: 137 SDVSIESRESVAKRVQEETGAILVHPFNNKNTISGQGTVSLELLEEVPEIDTIIVPISGG 196

Query: 192 GLISGISVAAKAINPAIRIFAAEPKGANDAAMSKAAGKIVTLPETTTIADGLRAFLGNLT 251
           GLISG+++AAKAINP+IRI AAEPKGA+D+A SKAAGKI+TLP T TIADGLRAFLG+LT
Sbjct: 197 GLISGVALAAKAINPSIRILAAEPKGADDSAQSKAAGKIITLPSTNTIADGLRAFLGDLT 256

Query: 252 WPIVRDLVDDVITVEDIEIVEAMRLCLEILKVVVEPSGAIGLAAVLSDSFKQNPSWKDCN 311
           WP+VRDLVDD+I V+D  IV+AM++C E+LKV VEPSGAIGLAA LSD FKQ+ +W + +
Sbjct: 257 WPVVRDLVDDIIVVDDNAIVDAMKMCYEMLKVAVEPSGAIGLAAALSDEFKQSSAWHESS 316

Query: 312 SIGIILSGGNVDLGMLWNSYKK 334
            IGII+SGGNVDLG+LW S  K
Sbjct: 317 KIGIIVSGGNVDLGVLWESLYK 338

BLAST of Csa5G154750 vs. Swiss-Prot
Match: SRR_ORYSI (Serine racemase OS=Oryza sativa subsp. indica GN=OsI_16936 PE=3 SV=1)

HSP 1 Score: 484.2 bits (1245), Expect = 1.2e-135
Identity = 234/322 (72.67%), Postives = 280/322 (86.96%), Query Frame = 1

Query: 12  YAADISSIKEARIRIRPFIHQTPVFSSETINAASGKQLFFKCECFQKGGAFKFRGACNAI 71
           YAADI SI+EA+ RI P++H+TPV SS +I+A  GKQLFFKCECFQK GAFK RGA N+I
Sbjct: 17  YAADIHSIREAQARIAPYVHKTPVLSSTSIDAIVGKQLFFKCECFQKAGAFKIRGASNSI 76

Query: 72  YSLDEGEAAKGVVTHSSGNHAAALSLAAKLRGIPAYIVIPENAPKCKVENVIRYGGQIIW 131
           ++LD+ EA+KGVVTHSSGNHAAA++LAAKLRGIPAYIVIP NAP CKV+NV RYGG IIW
Sbjct: 77  FALDDDEASKGVVTHSSGNHAAAVALAAKLRGIPAYIVIPRNAPACKVDNVKRYGGHIIW 136

Query: 132 SKSAIQSRESVAARVMQETGALLIHPYNDGRIISGQGTISLELLEQVPQLDTLIVPISGG 191
           S  +I+SRESVA RV +ETGA+L+HP+N+   ISGQGT+SLELLE+VP++DT+IVPISGG
Sbjct: 137 SDVSIESRESVAKRVQEETGAILVHPFNNKNTISGQGTVSLELLEEVPEIDTIIVPISGG 196

Query: 192 GLISGISVAAKAINPAIRIFAAEPKGANDAAMSKAAGKIVTLPETTTIADGLRAFLGNLT 251
           GLISG+++AAKAINP+IRI AAEPKGA+D+A SKAAGKI+TLP T TIADGLRAFLG+LT
Sbjct: 197 GLISGVALAAKAINPSIRILAAEPKGADDSAQSKAAGKIITLPSTNTIADGLRAFLGDLT 256

Query: 252 WPIVRDLVDDVITVEDIEIVEAMRLCLEILKVVVEPSGAIGLAAVLSDSFKQNPSWKDCN 311
           WP+VRDLVDD+I V+D  IV+AM++C E+LKV VEPSGAIGLAA LSD FKQ+ +W + +
Sbjct: 257 WPVVRDLVDDIIVVDDNAIVDAMKMCYEMLKVAVEPSGAIGLAAALSDEFKQSSAWHESS 316

Query: 312 SIGIILSGGNVDLGMLWNSYKK 334
            IGII+SGGNVDLG+LW S  K
Sbjct: 317 KIGIIVSGGNVDLGVLWESLYK 338

BLAST of Csa5G154750 vs. Swiss-Prot
Match: SRR_DICDI (Probable serine racemase OS=Dictyostelium discoideum GN=srr PE=3 SV=1)

HSP 1 Score: 307.8 bits (787), Expect = 1.5e-82
Identity = 155/314 (49.36%), Postives = 217/314 (69.11%), Query Frame = 1

Query: 16  ISSIKEARIRIRPFIHQTPVFSSETINAASGKQLFFKCECFQKGGAFKFRGACNAIYSLD 75
           +  IKEA  RI  +IH+TPV ++ TIN  +GK+L+FKCE  QK G+FK RGACNAI+SLD
Sbjct: 9   LKDIKEAHKRIEKYIHKTPVLTNSTINELAGKELYFKCENLQKTGSFKMRGACNAIFSLD 68

Query: 76  EGEAAKGVVTHSSGNHAAALSLAAKLRGIPAYIVIPENAPKCKVENVIRYGGQIIWSKSA 135
           E E +KGVVTHSSGNH  ALS A+K+R +  Y+V+PE+AP  K+  +  YG  +   K+ 
Sbjct: 69  EEELSKGVVTHSSGNHGQALSYASKVRCVKCYVVVPEDAPSVKLNAICGYGATVTKCKAT 128

Query: 136 IQSRESVAARVMQETGALLIHPYNDGRIISGQGTISLELLEQVPQLDTLIVPISGGGLIS 195
           +++RES   +++++    LIHP+++ ++I+GQGT SLEL+EQV  LD +I P+ GGGL+S
Sbjct: 129 LEARESNTKQLIEQHSCKLIHPFDNLQVIAGQGTASLELMEQVENLDAIITPVGGGGLLS 188

Query: 196 GISVAAKAINPAIRIFAAEPKGANDAAMSKAAGKIV--TLPETTTIADGLRAFLGNLTWP 255
           G  + AK++NP I++FAAEP GA+D   S  +G+I   T  +  TIADGL   +G+LT+P
Sbjct: 189 GTCITAKSLNPNIKVFAAEPLGADDTYRSLLSGEIQKHTPGKPNTIADGLLTTVGSLTFP 248

Query: 256 IVRDLVDDVITVEDIEIVEAMRLCLEILKVVVEPSGAIGLAAVLSDSFKQNPSWKDCNSI 315
           I+++  D VI V + EI  AM+L  E +K+++EPS A  LAA+L   FK     KD   +
Sbjct: 249 IIKENCDGVILVTEDEIKYAMKLVWERMKIIIEPSSATTLAAILKQEFKDK---KDIKKV 308

Query: 316 GIILSGGNVDLGML 328
           GII+SGGNVDL  +
Sbjct: 309 GIIISGGNVDLSSI 319

BLAST of Csa5G154750 vs. Swiss-Prot
Match: SRR_BOVIN (Serine racemase OS=Bos taurus GN=SRR PE=2 SV=1)

HSP 1 Score: 305.8 bits (782), Expect = 5.7e-82
Identity = 150/321 (46.73%), Postives = 217/321 (67.60%), Query Frame = 1

Query: 11  EYAADISSIKEARIRIRPFIHQTPVFSSETINAASGKQLFFKCECFQKGGAFKFRGACNA 70
           +Y    + +++A I IR FIH TPV +S  +N  +G+ LFFKCE FQK G+FK RGA NA
Sbjct: 4   QYCISFADVEKAHINIRDFIHLTPVLTSSILNQITGRNLFFKCELFQKTGSFKIRGALNA 63

Query: 71  IYSLDEG---EAAKGVVTHSSGNHAAALSLAAKLRGIPAYIVIPENAPKCKVENVIRYGG 130
           I  L      E  + VV HSSGNH  ALS AA+L GIPAY+++PE AP CK   +  YG 
Sbjct: 64  IRGLISAHPEEKPRAVVAHSSGNHGQALSFAARLEGIPAYVIVPETAPNCKKLAIQAYGA 123

Query: 131 QIIWSKSAIQSRESVAARVMQETGALLIHPYNDGRIISGQGTISLELLEQVPQLDTLIVP 190
            I++S+ + +SRE++  R+ +ET  +++HP  +  +I+GQGTI++E+L QVP +D L+VP
Sbjct: 124 SIVYSEQSEESRENITKRIAEETEGIMVHPNQEPAVIAGQGTIAMEVLNQVPLVDALVVP 183

Query: 191 ISGGGLISGISVAAKAINPAIRIFAAEPKGANDAAMSKAAGKIVTLP-ETTTIADGLRAF 250
           + GGG+++GI+V  KA+ P+++++AAEP  A+D   SK  G++   P    TIADG+++ 
Sbjct: 184 VGGGGMLAGIAVTVKALRPSVKVYAAEPLNADDCYQSKLKGELTPNPYPPETIADGIKSS 243

Query: 251 LGNLTWPIVRDLVDDVITVEDIEIVEAMRLCLEILKVVVEPSGAIGLAAVLSDSFKQNPS 310
           +G  TWPI+RDLVDDV TV + EI  A +L  E +K+++EP+  +G+A VLS  F+  P+
Sbjct: 244 IGLNTWPIIRDLVDDVFTVTEDEIKYATQLVWERMKLLIEPTAGVGVAVVLSQHFRTVPA 303

Query: 311 WKDCNSIGIILSGGNVDLGML 328
             +  +I I+LSGGNVDL  L
Sbjct: 304 --EVKNICIVLSGGNVDLTSL 322

BLAST of Csa5G154750 vs. TrEMBL
Match: D7TW10_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_02s0025g03670 PE=4 SV=1)

HSP 1 Score: 544.3 bits (1401), Expect = 1.1e-151
Identity = 258/333 (77.48%), Postives = 307/333 (92.19%), Query Frame = 1

Query: 1   MNVESQNKKKEYAADISSIKEARIRIRPFIHQTPVFSSETINAASGKQLFFKCECFQKGG 60
           M  E++ + K YAADISSI+EA++RI  FIH+TPV +SET+N+ +G+ LFFKCECFQKGG
Sbjct: 1   MEAENEIRSKRYAADISSIREAQVRINSFIHKTPVLTSETLNSLAGRGLFFKCECFQKGG 60

Query: 61  AFKFRGACNAIYSLDEGEAAKGVVTHSSGNHAAALSLAAKLRGIPAYIVIPENAPKCKVE 120
           AFKFRGACNAI+SLD+ +AAKGVVTHSSGNHAAALSLAAKLRGIPAYIVIPENAP+CKVE
Sbjct: 61  AFKFRGACNAIFSLDDEQAAKGVVTHSSGNHAAALSLAAKLRGIPAYIVIPENAPRCKVE 120

Query: 121 NVIRYGGQIIWSKSAIQSRESVAARVMQETGALLIHPYNDGRIISGQGTISLELLEQVPQ 180
           NVIRYGGQ+IWS++ +QSRE +A RV+QETGA+L+HPYNDGRIISGQGT+SLELLEQ PQ
Sbjct: 121 NVIRYGGQVIWSRATMQSREDIATRVLQETGAVLLHPYNDGRIISGQGTVSLELLEQAPQ 180

Query: 181 LDTLIVPISGGGLISGISVAAKAINPAIRIFAAEPKGANDAAMSKAAGKIVTLPETTTIA 240
           +DT++VPISGGGLISG+++AAK+INP+IR+FAAEPKGANDAA SK AG+I+TLPET TIA
Sbjct: 181 IDTIMVPISGGGLISGVALAAKSINPSIRVFAAEPKGANDAAQSKNAGRIITLPETNTIA 240

Query: 241 DGLRAFLGNLTWPIVRDLVDDVITVEDIEIVEAMRLCLEILKVVVEPSGAIGLAAVLSDS 300
           DGLRA LG+LTWP+VRDLVDD+I VED EI+EAM+LC EILKV VEPSGAIGLAAVLSDS
Sbjct: 241 DGLRASLGDLTWPVVRDLVDDIIVVEDKEIIEAMKLCYEILKVAVEPSGAIGLAAVLSDS 300

Query: 301 FKQNPSWKDCNSIGIILSGGNVDLGMLWNSYKK 334
           FK+NP+WK+C ++GI+LSGGNVDLG+LW+S  K
Sbjct: 301 FKKNPAWKNCRNVGIVLSGGNVDLGVLWDSLGK 333

BLAST of Csa5G154750 vs. TrEMBL
Match: B9RC97_RICCO (Serine racemase, putative OS=Ricinus communis GN=RCOM_1686380 PE=4 SV=1)

HSP 1 Score: 540.0 bits (1390), Expect = 2.0e-150
Identity = 259/333 (77.78%), Postives = 306/333 (91.89%), Query Frame = 1

Query: 1   MNVESQNKKKEYAADISSIKEARIRIRPFIHQTPVFSSETINAASGKQLFFKCECFQKGG 60
           M   S+   ++YAADISSI+EA  RI PFIH+TPV SSE++NA SG++LFFKCECFQKGG
Sbjct: 1   METRSRISTEKYAADISSIREAHARINPFIHKTPVLSSESLNALSGRKLFFKCECFQKGG 60

Query: 61  AFKFRGACNAIYSLDEGEAAKGVVTHSSGNHAAALSLAAKLRGIPAYIVIPENAPKCKVE 120
           AFKFRGACNAI+SLD+ + AKGVVTHSSGNHAAALSLAAKLRGIPAYIV+P+ AP+CKVE
Sbjct: 61  AFKFRGACNAIFSLDDDQTAKGVVTHSSGNHAAALSLAAKLRGIPAYIVVPKTAPRCKVE 120

Query: 121 NVIRYGGQIIWSKSAIQSRESVAARVMQETGALLIHPYNDGRIISGQGTISLELLEQVPQ 180
           NVIRYGGQ+IWS+ ++ SRESVA +V+QETGA+LIHPYND RIISGQGTISLELLEQ PQ
Sbjct: 121 NVIRYGGQVIWSEPSLHSRESVATKVVQETGAVLIHPYNDRRIISGQGTISLELLEQAPQ 180

Query: 181 LDTLIVPISGGGLISGISVAAKAINPAIRIFAAEPKGANDAAMSKAAGKIVTLPETTTIA 240
           +DT+IVPISGGGLISG+++AAK+INP+IRI AAEPKGANDAA SKAAG+I+TLPET TIA
Sbjct: 181 IDTIIVPISGGGLISGVALAAKSINPSIRILAAEPKGANDAAQSKAAGEIITLPETNTIA 240

Query: 241 DGLRAFLGNLTWPIVRDLVDDVITVEDIEIVEAMRLCLEILKVVVEPSGAIGLAAVLSDS 300
           DGLRA LG+LTWP+VRDLVDDVIT+ED EIVEAM+LC EILKV +EPSG IGLAA+LSDS
Sbjct: 241 DGLRASLGSLTWPVVRDLVDDVITLEDNEIVEAMKLCYEILKVAIEPSGVIGLAAILSDS 300

Query: 301 FKQNPSWKDCNSIGIILSGGNVDLGMLWNSYKK 334
           F++NP+WKDC++IGIILSGGNVDLG++W+S++K
Sbjct: 301 FQKNPNWKDCSNIGIILSGGNVDLGVIWDSFRK 333

BLAST of Csa5G154750 vs. TrEMBL
Match: A0A067K0X5_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_20610 PE=4 SV=1)

HSP 1 Score: 538.5 bits (1386), Expect = 5.8e-150
Identity = 260/333 (78.08%), Postives = 304/333 (91.29%), Query Frame = 1

Query: 1   MNVESQNKKKEYAADISSIKEARIRIRPFIHQTPVFSSETINAASGKQLFFKCECFQKGG 60
           M  ++Q   ++YAADISSI+EA++RI  F+H+TPV  SE++NA SG++LFFKCECFQKGG
Sbjct: 1   METKNQISTEKYAADISSIREAQLRINSFVHKTPVLISESLNAISGRELFFKCECFQKGG 60

Query: 61  AFKFRGACNAIYSLDEGEAAKGVVTHSSGNHAAALSLAAKLRGIPAYIVIPENAPKCKVE 120
           AFKFRGACNAI+SLD   AAKGVVTHSSGNHAAAL+LAAKLRGIPAY+VIP+NAPKCKVE
Sbjct: 61  AFKFRGACNAIFSLDGDRAAKGVVTHSSGNHAAALALAAKLRGIPAYVVIPKNAPKCKVE 120

Query: 121 NVIRYGGQIIWSKSAIQSRESVAARVMQETGALLIHPYNDGRIISGQGTISLELLEQVPQ 180
           NV+RYGG+IIWS+  IQSRES A +V++ETGA+L+HP+NDGRIISGQGTISLELLEQ PQ
Sbjct: 121 NVVRYGGKIIWSEPTIQSRESTATKVLRETGAVLVHPFNDGRIISGQGTISLELLEQAPQ 180

Query: 181 LDTLIVPISGGGLISGISVAAKAINPAIRIFAAEPKGANDAAMSKAAGKIVTLPETTTIA 240
           +DT+I PISGGGLISG+++AAK+INP+IR+ AAEPKGANDAA SKAAG+I+ LPET TIA
Sbjct: 181 IDTIIAPISGGGLISGVALAAKSINPSIRVLAAEPKGANDAAQSKAAGEIIILPETNTIA 240

Query: 241 DGLRAFLGNLTWPIVRDLVDDVITVEDIEIVEAMRLCLEILKVVVEPSGAIGLAAVLSDS 300
           DGLRAFLGNLTWPIVRD VDDVITVED EIVEAM+LC EILKV +EPSGAIGLAAVLSDS
Sbjct: 241 DGLRAFLGNLTWPIVRDFVDDVITVEDEEIVEAMKLCYEILKVAIEPSGAIGLAAVLSDS 300

Query: 301 FKQNPSWKDCNSIGIILSGGNVDLGMLWNSYKK 334
           F++NPS KDCN IGIILSGGNVDLG+LW+S+K+
Sbjct: 301 FQKNPSLKDCNHIGIILSGGNVDLGVLWDSFKR 333

BLAST of Csa5G154750 vs. TrEMBL
Match: A0A061G5P5_THECC (Serine racemase isoform 2 OS=Theobroma cacao GN=TCM_016234 PE=4 SV=1)

HSP 1 Score: 534.6 bits (1376), Expect = 8.3e-149
Identity = 254/325 (78.15%), Postives = 300/325 (92.31%), Query Frame = 1

Query: 9   KKEYAADISSIKEARIRIRPFIHQTPVFSSETINAASGKQLFFKCECFQKGGAFKFRGAC 68
           K +YAAD+SSI+EA+ RI  FIH+TPV +SE+++A SG++LFFKCECFQKGGAFKFRGAC
Sbjct: 5   KGKYAADLSSIREAQARINSFIHKTPVMTSESLDAISGRRLFFKCECFQKGGAFKFRGAC 64

Query: 69  NAIYSLDEGEAAKGVVTHSSGNHAAALSLAAKLRGIPAYIVIPENAPKCKVENVIRYGGQ 128
           NA++SLD+ +AAKGVVTHSSGNHAAAL+LAAKLRGIPA+IVIP+NAP+CKV+NV+RYGGQ
Sbjct: 65  NAVFSLDDHQAAKGVVTHSSGNHAAALALAAKLRGIPAHIVIPKNAPQCKVQNVVRYGGQ 124

Query: 129 IIWSKSAIQSRESVAARVMQETGALLIHPYNDGRIISGQGTISLELLEQVPQLDTLIVPI 188
           +IWS++ + SRE  A +V+QETGA+LIHPYNDGRIISGQGTISLELLEQ P++DT+IVPI
Sbjct: 125 VIWSEATVPSREKTATKVLQETGAVLIHPYNDGRIISGQGTISLELLEQAPEIDTIIVPI 184

Query: 189 SGGGLISGISVAAKAINPAIRIFAAEPKGANDAAMSKAAGKIVTLPETTTIADGLRAFLG 248
           SGGGLISG+++AAK+INPAIR+ AAEPKGANDAA SKAAG+IVTLPET T+ADGLRAFLG
Sbjct: 185 SGGGLISGVALAAKSINPAIRVLAAEPKGANDAAQSKAAGRIVTLPETNTVADGLRAFLG 244

Query: 249 NLTWPIVRDLVDDVITVEDIEIVEAMRLCLEILKVVVEPSGAIGLAAVLSDSFKQNPSWK 308
            LTWPIVRD VDD+ITV+D EI+EAMRLC EILKV VEPSGAIGLAAVLSDSF+ NP+WK
Sbjct: 245 ELTWPIVRDFVDDIITVDDTEIIEAMRLCYEILKVAVEPSGAIGLAAVLSDSFRNNPAWK 304

Query: 309 DCNSIGIILSGGNVDLGMLWNSYKK 334
           DCN IGIILSGGNVDL  LW+S++K
Sbjct: 305 DCNQIGIILSGGNVDLEKLWSSFRK 329

BLAST of Csa5G154750 vs. TrEMBL
Match: A0A0D2T695_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_008G246100 PE=4 SV=1)

HSP 1 Score: 530.4 bits (1365), Expect = 1.6e-147
Identity = 256/325 (78.77%), Postives = 297/325 (91.38%), Query Frame = 1

Query: 9   KKEYAADISSIKEARIRIRPFIHQTPVFSSETINAASGKQLFFKCECFQKGGAFKFRGAC 68
           K++YAAD+SSIKEAR RI  FI++TP+ +SE+++A SG++LFFKCECFQKGGAFKFRGAC
Sbjct: 5   KEKYAADLSSIKEARARISSFINKTPLMTSESLDAISGRRLFFKCECFQKGGAFKFRGAC 64

Query: 69  NAIYSLDEGEAAKGVVTHSSGNHAAALSLAAKLRGIPAYIVIPENAPKCKVENVIRYGGQ 128
           NAI+SLD+ +AAKGVVTHSSGNHAAAL+LAAKLRGI +YIVIP+NAP+CKV+NV+RYGGQ
Sbjct: 65  NAIFSLDDQQAAKGVVTHSSGNHAAALALAAKLRGITSYIVIPKNAPQCKVQNVVRYGGQ 124

Query: 129 IIWSKSAIQSRESVAARVMQETGALLIHPYNDGRIISGQGTISLELLEQVPQLDTLIVPI 188
           +IWS++ I+SRE  A  V QETGA LIHPYNDGRIISGQGTISLELLEQ P +DT+IVPI
Sbjct: 125 VIWSETTIKSREETATTVQQETGAALIHPYNDGRIISGQGTISLELLEQAPHIDTIIVPI 184

Query: 189 SGGGLISGISVAAKAINPAIRIFAAEPKGANDAAMSKAAGKIVTLPETTTIADGLRAFLG 248
           SGGGLISG+++AAK+INPAIR+ AAEPKGANDAA SKAAG+IVTLPET T+ADGLRAFLG
Sbjct: 185 SGGGLISGVALAAKSINPAIRVLAAEPKGANDAAQSKAAGRIVTLPETNTVADGLRAFLG 244

Query: 249 NLTWPIVRDLVDDVITVEDIEIVEAMRLCLEILKVVVEPSGAIGLAAVLSDSFKQNPSWK 308
             TWPIVRDLVDDVITV+D EI++AMRLC EILKV VEPSGAIGLAAVLSDSF+ NP+WK
Sbjct: 245 EFTWPIVRDLVDDVITVDDTEIIKAMRLCYEILKVAVEPSGAIGLAAVLSDSFRTNPAWK 304

Query: 309 DCNSIGIILSGGNVDLGMLWNSYKK 334
           DCN IGIILSGGNVDL +LWNS+ K
Sbjct: 305 DCNQIGIILSGGNVDLEVLWNSFSK 329

BLAST of Csa5G154750 vs. TAIR10
Match: AT4G11640.1 (AT4G11640.1 serine racemase)

HSP 1 Score: 503.4 bits (1295), Expect = 1.0e-142
Identity = 237/324 (73.15%), Postives = 292/324 (90.12%), Query Frame = 1

Query: 9   KKEYAADISSIKEARIRIRPFIHQTPVFSSETINAASGKQLFFKCECFQKGGAFKFRGAC 68
           +++YAADI SIKEA  RI+P+IH+TPV +SE++N+ SG+ LFFKCEC QKGGAFKFRGAC
Sbjct: 5   REKYAADILSIKEAHDRIKPYIHRTPVLTSESLNSISGRSLFFKCECLQKGGAFKFRGAC 64

Query: 69  NAIYSLDEGEAAKGVVTHSSGNHAAALSLAAKLRGIPAYIVIPENAPKCKVENVIRYGGQ 128
           NA+ SLD  +AAKGVVTHSSGNHAAALSLAAK++GIPAYIV+P+ APKCKV+NVIRYGG+
Sbjct: 65  NAVLSLDAEQAAKGVVTHSSGNHAAALSLAAKIQGIPAYIVVPKGAPKCKVDNVIRYGGK 124

Query: 129 IIWSKSAIQSRESVAARVMQETGALLIHPYNDGRIISGQGTISLELLEQVPQLDTLIVPI 188
           +IWS++ + SRE +A++V+QETG++LIHPYNDGRIISGQGTI+LELLEQ+ ++D ++VPI
Sbjct: 125 VIWSEATMSSREEIASKVLQETGSVLIHPYNDGRIISGQGTIALELLEQIQEIDAIVVPI 184

Query: 189 SGGGLISGISVAAKAINPAIRIFAAEPKGANDAAMSKAAGKIVTLPETTTIADGLRAFLG 248
           SGGGLISG+++AAK+I P+IRI AAEPKGA+DAA SK AGKI+TLP T TIADGLRA LG
Sbjct: 185 SGGGLISGVALAAKSIKPSIRIIAAEPKGADDAAQSKVAGKIITLPVTNTIADGLRASLG 244

Query: 249 NLTWPIVRDLVDDVITVEDIEIVEAMRLCLEILKVVVEPSGAIGLAAVLSDSFKQNPSWK 308
           +LTWP+VRDLVDDV+T+E+ EI+EAM++C EILKV VEPSGAIGLAAVLS+SF+ NPS +
Sbjct: 245 DLTWPVVRDLVDDVVTLEECEIIEAMKMCYEILKVSVEPSGAIGLAAVLSNSFRNNPSCR 304

Query: 309 DCNSIGIILSGGNVDLGMLWNSYK 333
           DC +IGI+LSGGNVDLG LW+S+K
Sbjct: 305 DCKNIGIVLSGGNVDLGSLWDSFK 328

BLAST of Csa5G154750 vs. TAIR10
Match: AT3G10050.1 (AT3G10050.1 L-O-methylthreonine resistant 1)

HSP 1 Score: 150.6 bits (379), Expect = 1.7e-36
Identity = 100/319 (31.35%), Postives = 166/319 (52.04%), Query Frame = 1

Query: 11  EYAADISSIKEARIRIRPFIHQTPVFSSETINAASGKQLFFKCECFQKGGAFKFRGACNA 70
           EY  +I S K   I I     ++P+  ++ ++   G +++ K E  Q   +FK RGA N 
Sbjct: 94  EYLTNILSTKVYDIAI-----ESPLQLAKKLSKRLGVRMYLKREDLQPVFSFKLRGAYNM 153

Query: 71  IYSLDEGEAAKGVVTHSSGNHAAALSLAAKLRGIPAYIVIPENAPKCKVENVIRYGGQII 130
           +  L   + AKGV+  S+GNHA  ++L+A   G  A IV+P   P+ K + V   G  ++
Sbjct: 154 MVKLPADQLAKGVICSSAGNHAQGVALSASKLGCTAVIVMPVTTPEIKWQAVENLGATVV 213

Query: 131 WSKSAIQSRESVAARVMQETGALLIHPYNDGRIISGQGTISLELLEQVP-QLDTLIVPIS 190
               +    ++ A    +E G   I P++   +I+GQGT+ +E+  Q    L  + VP+ 
Sbjct: 214 LFGDSYDQAQAHAKIRAEEEGLTFIPPFDHPDVIAGQGTVGMEITRQAKGPLHAIFVPVG 273

Query: 191 GGGLISGISVAAKAINPAIRIFAAEPKGANDAAMSKAAGKIVTLPETTTIADGLRA-FLG 250
           GGGLI+GI+   K ++P ++I   EP  AN  A+S   G+ V L +    ADG+    +G
Sbjct: 274 GGGLIAGIAAYVKRVSPEVKIIGVEPADANAMALSLHHGERVILDQVGGFADGVAVKEVG 333

Query: 251 NLTWPIVRDLVDDVITVEDIEIVEAMRLCLEILKVVVEPSGAIGLAAVLSDSFKQNPSWK 310
             T+ I R+L+D V+ V    I  +++   E  + ++EP+GA+ LA   ++++ +    K
Sbjct: 334 EETFRISRNLMDGVVLVTRDAICASIKDMFEEKRNILEPAGALALAG--AEAYCKYYGLK 393

Query: 311 DCNSIGIILSGGNVDLGML 328
           D N +  I SG N++   L
Sbjct: 394 DVNVVA-ITSGANMNFDKL 404

BLAST of Csa5G154750 vs. NCBI nr
Match: gi|778699683|ref|XP_011654760.1| (PREDICTED: serine racemase-like [Cucumis sativus])

HSP 1 Score: 652.1 bits (1681), Expect = 5.1e-184
Identity = 333/333 (100.00%), Postives = 333/333 (100.00%), Query Frame = 1

Query: 1   MNVESQNKKKEYAADISSIKEARIRIRPFIHQTPVFSSETINAASGKQLFFKCECFQKGG 60
           MNVESQNKKKEYAADISSIKEARIRIRPFIHQTPVFSSETINAASGKQLFFKCECFQKGG
Sbjct: 1   MNVESQNKKKEYAADISSIKEARIRIRPFIHQTPVFSSETINAASGKQLFFKCECFQKGG 60

Query: 61  AFKFRGACNAIYSLDEGEAAKGVVTHSSGNHAAALSLAAKLRGIPAYIVIPENAPKCKVE 120
           AFKFRGACNAIYSLDEGEAAKGVVTHSSGNHAAALSLAAKLRGIPAYIVIPENAPKCKVE
Sbjct: 61  AFKFRGACNAIYSLDEGEAAKGVVTHSSGNHAAALSLAAKLRGIPAYIVIPENAPKCKVE 120

Query: 121 NVIRYGGQIIWSKSAIQSRESVAARVMQETGALLIHPYNDGRIISGQGTISLELLEQVPQ 180
           NVIRYGGQIIWSKSAIQSRESVAARVMQETGALLIHPYNDGRIISGQGTISLELLEQVPQ
Sbjct: 121 NVIRYGGQIIWSKSAIQSRESVAARVMQETGALLIHPYNDGRIISGQGTISLELLEQVPQ 180

Query: 181 LDTLIVPISGGGLISGISVAAKAINPAIRIFAAEPKGANDAAMSKAAGKIVTLPETTTIA 240
           LDTLIVPISGGGLISGISVAAKAINPAIRIFAAEPKGANDAAMSKAAGKIVTLPETTTIA
Sbjct: 181 LDTLIVPISGGGLISGISVAAKAINPAIRIFAAEPKGANDAAMSKAAGKIVTLPETTTIA 240

Query: 241 DGLRAFLGNLTWPIVRDLVDDVITVEDIEIVEAMRLCLEILKVVVEPSGAIGLAAVLSDS 300
           DGLRAFLGNLTWPIVRDLVDDVITVEDIEIVEAMRLCLEILKVVVEPSGAIGLAAVLSDS
Sbjct: 241 DGLRAFLGNLTWPIVRDLVDDVITVEDIEIVEAMRLCLEILKVVVEPSGAIGLAAVLSDS 300

Query: 301 FKQNPSWKDCNSIGIILSGGNVDLGMLWNSYKK 334
           FKQNPSWKDCNSIGIILSGGNVDLGMLWNSYKK
Sbjct: 301 FKQNPSWKDCNSIGIILSGGNVDLGMLWNSYKK 333

BLAST of Csa5G154750 vs. NCBI nr
Match: gi|659073779|ref|XP_008437249.1| (PREDICTED: serine racemase [Cucumis melo])

HSP 1 Score: 637.1 bits (1642), Expect = 1.7e-179
Identity = 323/333 (97.00%), Postives = 328/333 (98.50%), Query Frame = 1

Query: 1   MNVESQNKKKEYAADISSIKEARIRIRPFIHQTPVFSSETINAASGKQLFFKCECFQKGG 60
           MN ESQNKKKEYAADISSIKEARIRIRPFIH+TPVFSSETINAASGK+LFFKCECFQKGG
Sbjct: 1   MNAESQNKKKEYAADISSIKEARIRIRPFIHETPVFSSETINAASGKRLFFKCECFQKGG 60

Query: 61  AFKFRGACNAIYSLDEGEAAKGVVTHSSGNHAAALSLAAKLRGIPAYIVIPENAPKCKVE 120
           AFK RGACNAIYSLDEGEAAKGVVTHSSGNHAAALSLAAKLRGIPAYIVIPENAPKCKVE
Sbjct: 61  AFKIRGACNAIYSLDEGEAAKGVVTHSSGNHAAALSLAAKLRGIPAYIVIPENAPKCKVE 120

Query: 121 NVIRYGGQIIWSKSAIQSRESVAARVMQETGALLIHPYNDGRIISGQGTISLELLEQVPQ 180
           NVIRYGGQIIWSK+ IQSRESVAARVMQETGALLIHPYNDGRIISGQGTISLELLEQVPQ
Sbjct: 121 NVIRYGGQIIWSKATIQSRESVAARVMQETGALLIHPYNDGRIISGQGTISLELLEQVPQ 180

Query: 181 LDTLIVPISGGGLISGISVAAKAINPAIRIFAAEPKGANDAAMSKAAGKIVTLPETTTIA 240
           LDTLIVPISGGGLISGISVAAKAINPAIRIFAAEPKGAND AMSKAAGKIVTLPETTTIA
Sbjct: 181 LDTLIVPISGGGLISGISVAAKAINPAIRIFAAEPKGANDVAMSKAAGKIVTLPETTTIA 240

Query: 241 DGLRAFLGNLTWPIVRDLVDDVITVEDIEIVEAMRLCLEILKVVVEPSGAIGLAAVLSDS 300
           DGLRAFLG+LTWPIVRDLVDDVITVED EI+EAMRLCLEILKVVVEPSGAIGLAAVLSDS
Sbjct: 241 DGLRAFLGDLTWPIVRDLVDDVITVEDTEIIEAMRLCLEILKVVVEPSGAIGLAAVLSDS 300

Query: 301 FKQNPSWKDCNSIGIILSGGNVDLGMLWNSYKK 334
           FKQNPSWKDCNSIGIILSGGNVDLGMLWNSYKK
Sbjct: 301 FKQNPSWKDCNSIGIILSGGNVDLGMLWNSYKK 333

BLAST of Csa5G154750 vs. NCBI nr
Match: gi|645266985|ref|XP_008238865.1| (PREDICTED: serine racemase [Prunus mume])

HSP 1 Score: 550.4 bits (1417), Expect = 2.1e-153
Identity = 266/334 (79.64%), Postives = 312/334 (93.41%), Query Frame = 1

Query: 1   MNVESQNKKKEYAADISSIKEARIRIRPFIHQTPVFSSETINAASGKQLFFKCECFQKGG 60
           M  ESQ ++ +YAADISSI+EA+ RI+PF+HQTPV SSE++NA +G+QLFFKCECFQKGG
Sbjct: 1   MEAESQMREVKYAADISSIREAQARIKPFVHQTPVLSSESLNALAGRQLFFKCECFQKGG 60

Query: 61  AFKFRGACNAIYSLDEGEAAKGVVTHSSGNHAAALSLAAKLRGIPAYIVIPENAPKCKVE 120
           AFKFRGACNA++SLD+ +A KGVVTHSSGNHAAALSLAAKLRGIPAYIVIP+NAPKCKVE
Sbjct: 61  AFKFRGACNAVFSLDDDQAVKGVVTHSSGNHAAALSLAAKLRGIPAYIVIPKNAPKCKVE 120

Query: 121 NVIRYGGQIIWSKSAIQSRESVAARVMQETGALLIHPYNDGRIISGQGTISLELLE-QVP 180
           NVIRYGGQ+IWS+  +QSRES AA+V+QETGA+L+HPYND RIISGQGTISLELLE QVP
Sbjct: 121 NVIRYGGQVIWSEPTMQSRESTAAKVLQETGAVLLHPYNDRRIISGQGTISLELLEQQVP 180

Query: 181 QLDTLIVPISGGGLISGISVAAKAINPAIRIFAAEPKGANDAAMSKAAGKIVTLPETTTI 240
           QLDT+IVPISGGGLISG+++AAK+INPAIR+ AAEP+GANDAA SKAAG+I+TLPET T+
Sbjct: 181 QLDTIIVPISGGGLISGVALAAKSINPAIRVLAAEPEGANDAAQSKAAGRIITLPETNTV 240

Query: 241 ADGLRAFLGNLTWPIVRDLVDDVITVEDIEIVEAMRLCLEILKVVVEPSGAIGLAAVLSD 300
           ADGLRAFLG+LTWP+VRDLVD +ITVED EI+ AM+LC EILKVVVEPSGAIGLAAVLSD
Sbjct: 241 ADGLRAFLGDLTWPVVRDLVDGIITVEDKEIIHAMKLCYEILKVVVEPSGAIGLAAVLSD 300

Query: 301 SFKQNPSWKDCNSIGIILSGGNVDLGMLWNSYKK 334
           SFK+N +WKDC++IGIILSGGNVDLG+LW++Y+K
Sbjct: 301 SFKKNSAWKDCSNIGIILSGGNVDLGILWDAYRK 334

BLAST of Csa5G154750 vs. NCBI nr
Match: gi|470125543|ref|XP_004298761.1| (PREDICTED: serine racemase isoform X3 [Fragaria vesca subsp. vesca])

HSP 1 Score: 546.6 bits (1407), Expect = 3.0e-152
Identity = 267/334 (79.94%), Postives = 306/334 (91.62%), Query Frame = 1

Query: 1   MNVESQNKKKEYAADISSIKEARIRIRPFIHQTPVFSSETINAASGKQLFFKCECFQKGG 60
           M  ESQ ++ +YAADISSIKEA+ RI+PFIH+TPV SSET+N  +G+QLFFKCECFQKGG
Sbjct: 1   MEAESQVREVKYAADISSIKEAQARIKPFIHETPVLSSETLNLIAGRQLFFKCECFQKGG 60

Query: 61  AFKFRGACNAIYSLDEGEAAKGVVTHSSGNHAAALSLAAKLRGIPAYIVIPENAPKCKVE 120
           AFKFRGACNA++SLD+ +AAKGVVTHSSGNHAAALSLAAKLRGIPAYIVIP+NAPKCKVE
Sbjct: 61  AFKFRGACNAVFSLDDDQAAKGVVTHSSGNHAAALSLAAKLRGIPAYIVIPKNAPKCKVE 120

Query: 121 NVIRYGGQIIWSKSAIQSRESVAARVMQETGALLIHPYNDGRIISGQGTISLELLE-QVP 180
           NV+RYGGQ+IWS+  +QSRES AA+V+QETGA+L+HPYND RIISGQGTISLELLE QVP
Sbjct: 121 NVMRYGGQVIWSEPTMQSRESTAAKVLQETGAVLLHPYNDERIISGQGTISLELLEQQVP 180

Query: 181 QLDTLIVPISGGGLISGISVAAKAINPAIRIFAAEPKGANDAAMSKAAGKIVTLPETTTI 240
           QLDT+IVPISGGGLISG+++AAK INPAIR+ AAEPKGANDAA SK AG+I+TLPET T+
Sbjct: 181 QLDTIIVPISGGGLISGVALAAKYINPAIRVLAAEPKGANDAAQSKEAGRIITLPETNTV 240

Query: 241 ADGLRAFLGNLTWPIVRDLVDDVITVEDIEIVEAMRLCLEILKVVVEPSGAIGLAAVLSD 300
           ADGLRAFLG+LTWP+VRDLVD VITVED EI+ AM+LC EILKV VEPSGAIGLAAVLSD
Sbjct: 241 ADGLRAFLGDLTWPVVRDLVDGVITVEDKEIINAMKLCYEILKVAVEPSGAIGLAAVLSD 300

Query: 301 SFKQNPSWKDCNSIGIILSGGNVDLGMLWNSYKK 334
           SFK N +WKDC +IGI+LSGGNVDLG+LW+SY K
Sbjct: 301 SFKNNTAWKDCTNIGIVLSGGNVDLGILWDSYTK 334

BLAST of Csa5G154750 vs. NCBI nr
Match: gi|225426387|ref|XP_002269706.1| (PREDICTED: serine racemase isoform X1 [Vitis vinifera])

HSP 1 Score: 544.3 bits (1401), Expect = 1.5e-151
Identity = 258/333 (77.48%), Postives = 307/333 (92.19%), Query Frame = 1

Query: 1   MNVESQNKKKEYAADISSIKEARIRIRPFIHQTPVFSSETINAASGKQLFFKCECFQKGG 60
           M  E++ + K YAADISSI+EA++RI  FIH+TPV +SET+N+ +G+ LFFKCECFQKGG
Sbjct: 1   MEAENEIRSKRYAADISSIREAQVRINSFIHKTPVLTSETLNSLAGRGLFFKCECFQKGG 60

Query: 61  AFKFRGACNAIYSLDEGEAAKGVVTHSSGNHAAALSLAAKLRGIPAYIVIPENAPKCKVE 120
           AFKFRGACNAI+SLD+ +AAKGVVTHSSGNHAAALSLAAKLRGIPAYIVIPENAP+CKVE
Sbjct: 61  AFKFRGACNAIFSLDDEQAAKGVVTHSSGNHAAALSLAAKLRGIPAYIVIPENAPRCKVE 120

Query: 121 NVIRYGGQIIWSKSAIQSRESVAARVMQETGALLIHPYNDGRIISGQGTISLELLEQVPQ 180
           NVIRYGGQ+IWS++ +QSRE +A RV+QETGA+L+HPYNDGRIISGQGT+SLELLEQ PQ
Sbjct: 121 NVIRYGGQVIWSRATMQSREDIATRVLQETGAVLLHPYNDGRIISGQGTVSLELLEQAPQ 180

Query: 181 LDTLIVPISGGGLISGISVAAKAINPAIRIFAAEPKGANDAAMSKAAGKIVTLPETTTIA 240
           +DT++VPISGGGLISG+++AAK+INP+IR+FAAEPKGANDAA SK AG+I+TLPET TIA
Sbjct: 181 IDTIMVPISGGGLISGVALAAKSINPSIRVFAAEPKGANDAAQSKNAGRIITLPETNTIA 240

Query: 241 DGLRAFLGNLTWPIVRDLVDDVITVEDIEIVEAMRLCLEILKVVVEPSGAIGLAAVLSDS 300
           DGLRA LG+LTWP+VRDLVDD+I VED EI+EAM+LC EILKV VEPSGAIGLAAVLSDS
Sbjct: 241 DGLRASLGDLTWPVVRDLVDDIIVVEDKEIIEAMKLCYEILKVAVEPSGAIGLAAVLSDS 300

Query: 301 FKQNPSWKDCNSIGIILSGGNVDLGMLWNSYKK 334
           FK+NP+WK+C ++GI+LSGGNVDLG+LW+S  K
Sbjct: 301 FKKNPAWKNCRNVGIVLSGGNVDLGVLWDSLGK 333

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
SRR_ARATH1.9e-14173.15Serine racemase OS=Arabidopsis thaliana GN=SR PE=1 SV=1[more]
SRR_ORYSJ1.2e-13572.67Serine racemase OS=Oryza sativa subsp. japonica GN=SERR PE=1 SV=2[more]
SRR_ORYSI1.2e-13572.67Serine racemase OS=Oryza sativa subsp. indica GN=OsI_16936 PE=3 SV=1[more]
SRR_DICDI1.5e-8249.36Probable serine racemase OS=Dictyostelium discoideum GN=srr PE=3 SV=1[more]
SRR_BOVIN5.7e-8246.73Serine racemase OS=Bos taurus GN=SRR PE=2 SV=1[more]
Match NameE-valueIdentityDescription
D7TW10_VITVI1.1e-15177.48Putative uncharacterized protein OS=Vitis vinifera GN=VIT_02s0025g03670 PE=4 SV=... [more]
B9RC97_RICCO2.0e-15077.78Serine racemase, putative OS=Ricinus communis GN=RCOM_1686380 PE=4 SV=1[more]
A0A067K0X5_JATCU5.8e-15078.08Uncharacterized protein OS=Jatropha curcas GN=JCGZ_20610 PE=4 SV=1[more]
A0A061G5P5_THECC8.3e-14978.15Serine racemase isoform 2 OS=Theobroma cacao GN=TCM_016234 PE=4 SV=1[more]
A0A0D2T695_GOSRA1.6e-14778.77Uncharacterized protein OS=Gossypium raimondii GN=B456_008G246100 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT4G11640.11.0e-14273.15 serine racemase[more]
AT3G10050.11.7e-3631.35 L-O-methylthreonine resistant 1[more]
Match NameE-valueIdentityDescription
gi|778699683|ref|XP_011654760.1|5.1e-184100.00PREDICTED: serine racemase-like [Cucumis sativus][more]
gi|659073779|ref|XP_008437249.1|1.7e-17997.00PREDICTED: serine racemase [Cucumis melo][more]
gi|645266985|ref|XP_008238865.1|2.1e-15379.64PREDICTED: serine racemase [Prunus mume][more]
gi|470125543|ref|XP_004298761.1|3.0e-15279.94PREDICTED: serine racemase isoform X3 [Fragaria vesca subsp. vesca][more]
gi|225426387|ref|XP_002269706.1|1.5e-15177.48PREDICTED: serine racemase isoform X1 [Vitis vinifera][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000634Ser/Thr_deHydtase_PyrdxlP-BS
IPR001926PLP-dep
Vocabulary: Biological Process
TermDefinition
GO:0006520cellular amino acid metabolic process
Vocabulary: Molecular Function
TermDefinition
GO:0030170pyridoxal phosphate binding
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006534 cysteine metabolic process
biological_process GO:0070178 D-serine metabolic process
biological_process GO:0006094 gluconeogenesis
biological_process GO:0006544 glycine metabolic process
biological_process GO:0006563 L-serine metabolic process
biological_process GO:0006566 threonine metabolic process
biological_process GO:0006520 cellular amino acid metabolic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0008721 D-serine ammonia-lyase activity
molecular_function GO:0003941 L-serine ammonia-lyase activity
molecular_function GO:0004794 L-threonine ammonia-lyase activity
molecular_function GO:0030170 pyridoxal phosphate binding
molecular_function GO:0030378 serine racemase activity
This gene is associated with the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
CU154773cucumber EST collection version 3.0transcribed_cluster
CU171800cucumber EST collection version 3.0transcribed_cluster

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csa5G154750.1Csa5G154750.1mRNA


The following transcribed_cluster feature(s) are associated with this gene:

Feature NameUnique NameType
CU154773CU154773transcribed_cluster
CU171800CU171800transcribed_cluster


Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000634Serine/threonine dehydratase, pyridoxal-phosphate-binding sitePROSITEPS00165DEHYDRATASE_SER_THRcoord: 54..67
scor
IPR001926Tryptophan synthase beta subunit-like PLP-dependent enzymePFAMPF00291PALPcoord: 27..300
score: 2.5
IPR001926Tryptophan synthase beta subunit-like PLP-dependent enzymeunknownSSF53686Tryptophan synthase beta subunit-like PLP-dependent enzymescoord: 16..326
score: 6.68
NoneNo IPR availableGENE3DG3DSA:3.40.50.1100coord: 81..326
score: 1.9
NoneNo IPR availablePANTHERPTHR10314SER/THR DEHYDRATASE, TRP SYNTHASEcoord: 15..327
score: 2.8E
NoneNo IPR availablePANTHERPTHR10314:SF36SERINE RACEMASEcoord: 15..327
score: 2.8E