Csa4G496760 (gene) Cucumber (Chinese Long) v2

BLAST of Csa4G496760 vs. Swiss-Prot
Match: PAD4_ARATH (Lipase-like PAD4 OS=Arabidopsis thaliana GN=PAD4 PE=1 SV=1)

HSP 1 Score: 305.1 bits (780), Expect = 1.8e-81
Identity = 173/426 (40.61%), Postives = 248/426 (58.22%), Query Frame = 1

Query: 189 GKFCHVVSNHDIMPRLLS-TPLSSLSPKLHILLRYWHLSMASPTFGK--LATQLTEREKE 248
           GK   V++ H     L + T L  LS       R + ++  SP  G   L+T ++     
Sbjct: 108 GKKLVVITGHSTGGALAAFTALWLLSQSSPPSFRVFCITFGSPLLGNQSLSTSISRSRLA 167

Query: 249 ELF-HIVLAHSNRISDLGEGTVQSQFWPFGNFFFCSEHGAICLDNAISVLKMLYLMLKTS 308
             F H+V  H     DL   +   QFWPFG + FCS+ G +CLDNA SV  M  ++  T+
Sbjct: 168 HNFCHVVSIH-----DLVPRSSNEQFWPFGTYLFCSDKGGVCLDNAGSVRLMFNILNTTA 227

Query: 309 APNLSIEDHLNYGYHVKKVGVQYMERKNFNSSCPPNSSYEAGLALALQSAGIPFQDEVAQ 368
             N   E+H  YG++V  +   +++ ++F     P++SY+AG+ALA+++ G    D    
Sbjct: 228 TQNT--EEHQRYGHYVFTLSHMFLKSRSFLGGSIPDNSYQAGVALAVEALGFSNDDTSGV 287

Query: 369 IAEHCLRTASRIGQTPNMNAAKLAISLSKITPYRAEIEWYKASCEEADNQLGYYDCFKKE 428
           + + C+ TA+RI + P + +A+LA  L+ + P R EI+WYK  C+ ++ QLGYYD FK+ 
Sbjct: 288 LVKECIETATRIVRAPILRSAELANELASVLPARLEIQWYKDRCDASEEQLGYYDFFKRY 347

Query: 429 DASVRHDRVNMNRHKLATFWNRVINMWENNELPPDFNTRAKWVNASQFYKLLVEPLDIAE 488
               R  +VNM+R +LA FW+ VI M E NELP DF+   KW+ ASQFY+LL EPLDIA 
Sbjct: 348 SLK-RDFKVNMSRIRLAKFWDTVIKMVETNELPFDFHLGKKWIYASQFYQLLAEPLDIAN 407

Query: 489 YYHRDMHIVHGHYLKCGRERRYEIFDKWWRGREVTEEGNTQRMKYASLTQDSCFWARLEE 548
           +Y        GHYL+  R +RYE+ DKW +G +V EE    R +YAS TQD+CFWA+LE+
Sbjct: 408 FYKNRDIKTGGHYLEGNRPKRYEVIDKWQKGVKVPEE--CVRSRYASTTQDTCFWAKLEQ 467

Query: 549 AKDLL-EIIKRDGDVRKLAPIWKSLENFERYARGLIERKEVSKDVIAKNSSYTLWAQELR 608
           AK+ L E  K   D ++ + + + +  FE YA  L+ +KEVS DV AKNSSY++W   L+
Sbjct: 468 AKEWLDEARKESSDPQRRSLLREKIVPFESYANTLVTKKEVSLDVKAKNSSYSVWEANLK 523

Query: 609 ALKLNM 610
             K  M
Sbjct: 528 EFKCKM 523

HSP 2 Score: 110.9 bits (276), Expect = 4.9e-23
Identity = 76/200 (38.00%), Postives = 103/200 (51.50%), Query Frame = 1

Query: 8   FESCHVMAALLGSTPLLLQSWEFCAAANAASPESFTTVVIDDVA---YVGFSGVQVLPRC 67
           FE+  + A+++ STPL   SW  C  AN        ++ I D+A   YV    V ++   
Sbjct: 6   FETSELQASVMISTPLFTDSWSSCNTANCNG-----SIKIHDIAGITYVAIPAVSMI--- 65

Query: 68  GGGGRELVALDGEGVEAELFWPLNRHREELQEPAMADSGILKMFVDIYTHKNL-VETITK 127
                +L  L G  V  ++ +P     E L    M D+ ILK+F+ +   + L +E + K
Sbjct: 66  -----QLGNLVGLPVTGDVLFPGLSSDEPLP---MVDAAILKLFLQLKIKEGLELELLGK 125

Query: 128 VMERSKSIVITGHSLGGAAATLCTLWLLSFLHTKTHHHPILCITFGSPLIGNESLSRAIQ 187
                K +VITGHS GGA A    LWLLS   +      + CITFGSPL+GN+SLS +I 
Sbjct: 126 -----KLVVITGHSTGGALAAFTALWLLS--QSSPPSFRVFCITFGSPLLGNQSLSTSIS 182

Query: 188 RERWCGKFCHVVSNHDIMPR 204
           R R    FCHVVS HD++PR
Sbjct: 186 RSRLAHNFCHVVSIHDLVPR 182

BLAST of Csa4G496760 vs. Swiss-Prot
Match: EDSBC_ARATH (Protein EDS1B OS=Arabidopsis thaliana GN=EDS1B PE=1 SV=1)

HSP 1 Score: 149.8 bits (377), Expect = 9.5e-35
Identity = 127/521 (24.38%), Postives = 225/521 (43.19%), Query Frame = 1

Query: 120 TITKVMERSKSIVITGHSLGGAAATLCTLWLLS--FLHTKTHHHPILCITFGSPLIGNES 179
           ++   ++R + +V TGHS GGA A L T+W L   F+          C+TFG+PL+G+  
Sbjct: 106 SVQSAVDRKQQVVFTGHSFGGATAILATVWYLETYFIRDAYAAPEPRCVTFGAPLVGDYI 165

Query: 180 LSRAIQRERWCGKFCHVVSNHDIMPRLLSTPLSSLSPKLHILLRYWHLSMAS-PTFGKLA 239
              A+ RE W   F + V+  DI+PR++    +++   L  +L     + A      ++ 
Sbjct: 166 FKHALGRENWSRFFVNFVTRFDIVPRIMLARKTTIEQTLSYVLGKLDSTRAPIHESDQVI 225

Query: 240 TQLTEREKEELFHIVLAHSNRISDLGEGTVQ--------SQFWPFGNFFFCSEHGAICLD 299
           T+   R   + + +      ++   GE  ++        S + P G F F ++   + ++
Sbjct: 226 TEFYTRVMRDTYTVASKAVCQLIGNGEAFLETLSSFYELSPYRPVGTFVFSTQKRLVVVN 285

Query: 300 NAISVLKMLYLMLKTS-------APNLSIEDHLNYGYHVKKVGVQYMERKNFNSSCPPNS 359
           N+ ++L+ML+   +++        P LSI DH  Y   V+ +G++ +   + ++   P  
Sbjct: 286 NSDAILQMLFYTCQSNDEQELSVIPFLSIRDHHGYEELVQSIGIKLLNHLDLHN---PLL 345

Query: 360 SYEAGLALALQSAGIPFQDEVAQIAEHCLRTASRIGQTPNMNAAKLAISLSKITPYRAEI 419
             E  +  AL   G+  +      A  C+  A    +    N  K+     +I      I
Sbjct: 346 DGENSIGSALDDLGMSTR------ARQCIHAALEAEKQRVENQKKIETKRDQIVERLTWI 405

Query: 420 -EWYKASCEEADNQLGYYDCFKKEDASVRHD-RVNMNRHKLATFWNRVINMWENNELPPD 479
            E YK  C+   N  GYYD FK  D++  +D + N+ R +LA  ++ V+ + +  +LP  
Sbjct: 406 VEVYKPKCQAHKN--GYYDSFK--DSNEENDFKANVKRVELAGIFDEVLGLVKKGQLPDG 465

Query: 480 FNTRAKWVNASQFYKLLVEPLDIAEYYHRDMHIVHGHYLKCGRERRYEIFDKWWRGREVT 539
           F     W+N +  Y+ L+EPLDI+ Y+ +  +   G Y+  GR  RY+   + +    + 
Sbjct: 466 FEGSRGWINLATQYRRLIEPLDISNYHGQLKNEDTGPYMLHGRPSRYKYAQRGYEHDILK 525

Query: 540 EEGNTQRMKYAS--------LTQD-------------SCFWARLEEAKDLLEIIKRDGDV 599
             G   +  + S        L QD             SCFWA +EE K            
Sbjct: 526 PTGMIAKDVFWSKVNGLNLGLQQDIQEILKNSGSECGSCFWAEVEELKG----------- 585

BLAST of Csa4G496760 vs. Swiss-Prot
Match: EDS1C_ARATH (Protein EDS1 OS=Arabidopsis thaliana GN=EDS1 PE=1 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 3.4e-32
Identity = 133/502 (26.49%), Postives = 215/502 (42.83%), Query Frame = 1

Query: 98  AMADSGILKMFVDIYTHKNLVETITKVMERS-KSIVITGHSLGGAAATLCTLWLLS--FL 157
           A  +   LK    I   +   +   ++  RS K IV TGHS GGA A L T+W L   F+
Sbjct: 83  ATVNEAFLKNLEAIIDPRTSFQASVEMAVRSRKQIVFTGHSSGGATAILATVWYLEKYFI 142

Query: 158 HTKTHHHPILCITFGSPLIGNESLSRAIQRERWCGKFCHVVSNHDIMPRLLSTPLSSLSP 217
                +    C+TFG+PL+G+   S A+ RE+W   F + VS  DI+PR++    +S+  
Sbjct: 143 RNPNVYLEPRCVTFGAPLVGDSIFSHALGREKWSRFFVNFVSRFDIVPRIMLARKASVEE 202

Query: 218 KL-HILLRYWHLSMASPTFGKLATQLTEREKEELFHIVLAHSNRISD------------- 277
            L H+L      +   P   K + Q +E+   E +  V+  ++ +++             
Sbjct: 203 TLPHVL------AQLDPR--KSSVQESEQRITEFYTRVMRDTSTVANQAVCELTGSAEAF 262

Query: 278 ---LGEGTVQSQFWPFGNFFFCSEHGAICLDNAISVLKMLYLMLKTS-------APNLSI 337
              L      S + P G F F +E   + ++N+ ++L+ML+   + S        P  SI
Sbjct: 263 LETLSSFLELSPYRPAGTFVFSTEKRLVAVNNSDAILQMLFYTSQASDEQEWSLIPFRSI 322

Query: 338 EDHLNYGYHVKKVGVQYMERKNFNSSCPPNSSYEAGLALALQSAGIPFQDEVAQIAEHCL 397
            DH +Y   V+ +G     +K FN     NS       L + + G  +    A + E   
Sbjct: 323 RDHHSYEELVQSMG-----KKLFNHLDGENSIESTLNDLGVSTRGRQYVQ--AALEEEKK 382

Query: 398 RTASRIGQTPNMNAAKLAISLSKITPYRAEIEW----YKASCEEADNQLGYYDCFKKEDA 457
           R  ++          K  I + +   +  ++ W    YK  C+   N  GYYD FK  + 
Sbjct: 383 RVENQ----------KKIIQVIEQERFLKKLAWIEDEYKPKCQAHKN--GYYDSFKVSNE 442

Query: 458 SVRHDRVNMNRHKLATFWNRVINMWENNELPPDFNTRAKWVNASQFYKLLVEPLDIAEYY 517
                + N+ R +LA  ++ V+ + +  +LP +F     W+  +  Y+ LVEPLDIA Y+
Sbjct: 443 E-NDFKANVKRAELAGVFDEVLGLMKKCQLPDEFEGDIDWIKLATRYRRLVEPLDIANYH 502

Query: 518 HRDMHIVHGHYLKCGR-------ERRYEIF----------DKWWR-----GREVTEEGNT 547
               +   G Y+K GR       +R YE +          D +W         +  E   
Sbjct: 503 RHLKNEDTGPYMKRGRPTRYIYAQRGYEHYILKPNGMIAEDVFWNKVNGLNLGLQLEEIQ 556

BLAST of Csa4G496760 vs. Swiss-Prot
Match: EDS1L_ARATH (Protein EDS1L OS=Arabidopsis thaliana GN=EDS1 PE=1 SV=1)

HSP 1 Score: 137.1 bits (344), Expect = 6.4e-31
Identity = 122/479 (25.47%), Postives = 202/479 (42.17%), Query Frame = 1

Query: 120 TITKVMERSKSIVITGHSLGGAAATLCTLWLLS--FLHTKTHHHPILCITFGSPLIGNES 179
           ++   +   K IV TGHS GGA A L T+W L   F+     +    C+TFG+PL+G+  
Sbjct: 106 SVEMAVRSRKQIVFTGHSSGGATAILATVWYLEKYFIRNPNVYLEPRCVTFGAPLVGDSI 165

Query: 180 LSRAIQRERWCGKFCHVVSNHDIMPRLLSTPLSSLSPKL-HILLRYWHLSMASPTFGKLA 239
            S A+ RE+W   F + V+  DI+PR+     +S+   L H+L      +   P     +
Sbjct: 166 FSHALGREKWSRFFVNFVTRFDIVPRITLARKASVEETLPHVL------AQLDPR--NSS 225

Query: 240 TQLTEREKEELFHIVLAHSNRISD----------------LGEGTVQSQFWPFGNFFFCS 299
            Q +E+   E +  V+  ++ +++                L      S + P G F F +
Sbjct: 226 VQESEQRITEFYTSVMRDTSTVANQAVCELTGSAEAILETLSSFLELSPYRPAGTFVFST 285

Query: 300 EHGAICLDNAISVLKMLYLMLKTS-------APNLSIEDHLNYGYHVKKVGVQYMERKNF 359
           E   + ++N+ ++L+ML+   + S        P  SI DH +Y   V+ +G+     K F
Sbjct: 286 EKRLVAVNNSDAILQMLFYTCQASDEQEWSLIPFRSIRDHHSYEELVQSMGM-----KLF 345

Query: 360 NSSCPPNSSYEAGLALALQSAGIPFQDEVAQIAEHCLRTASRIGQTPNMNAAKLAISLSK 419
           N     NS   +   L + + G  +    A + E   R  ++          K  I + +
Sbjct: 346 NHLDGENSIESSLNDLGVSTRGRQYVQ--AALEEEKKRVENQ----------KKIIQVIQ 405

Query: 420 ITPYRAEIEW----YKASCEEADNQLGYYDCFKKEDASVRHDRVNMNRHKLATFWNRVIN 479
              +  ++ W    YK  C+   N  GYYD FK  +      + N+ R +LA  ++ V+ 
Sbjct: 406 QERFLKKLAWIEDEYKPKCQAHKN--GYYDSFKVSNEE-NDFKANVKRAELAGVFDEVLG 465

Query: 480 MWENNELPPDFNTRAKWVNASQFYKLLVEPLDIAEYYHRDMHIVHGHYLKCGRERRYEIF 539
           + +  +LP +F     W+  +  Y+ LVEPLDIA Y+    +   G Y+K GR  RY   
Sbjct: 466 LLKKCQLPDEFEGDIDWIKLATRYRRLVEPLDIANYHRHLKNEDTGPYMKRGRPTRYIYA 525

Query: 540 DKWWRGREVTEEGN----------------------TQRMKYASLTQDSCFWARLEEAK 547
            + +    +   G                        + +K +     SCFWA +EE K
Sbjct: 526 QRGYEHHILKPNGMIAEDVFWNKVNGLNLGLQLEEIQETLKNSGSECGSCFWAEVEELK 556

BLAST of Csa4G496760 vs. Swiss-Prot
Match: SG101_ARATH (Senescence-associated carboxylesterase 101 OS=Arabidopsis thaliana GN=SAG101 PE=1 SV=1)

HSP 1 Score: 100.9 bits (250), Expect = 5.1e-20
Identity = 73/252 (28.97%), Postives = 116/252 (46.03%), Query Frame = 1

Query: 365 IAEHCLRTASRIGQTPNMNAAKLAISLSKITPYRAEIEWYKASCEEADNQLGYYDCFKKE 424
           I E  ++   +  +  N+    +   L+ +    A IEWYK  C+E   ++GYYD FK +
Sbjct: 271 IPEDVIKGIEKRAEMKNLRFDMMFKKLNDMKISMAYIEWYKKKCKEV--KIGYYDRFKTQ 330

Query: 425 DA--SVRHDRVNMNRHK--LATFWNRVINMWENNELPPDFNTRAKWVNASQFYKLLVEPL 484
            A  S   D    N HK  L  FW  V+   E          + +++ +   Y+ ++EPL
Sbjct: 331 LAFPSKEFDINIKNHHKSELNRFWKSVVEEVERRPQSDASILKRRFLFSGNNYRRMIEPL 390

Query: 485 DIAEYYHRDMHIVHGHYLKCGRERRYEIFDKWWRGREVTEEGNTQRMKYAS--LTQDSCF 544
           DIAEYY          Y   GR   Y + +KW+    +  E    + +  S  LT DSCF
Sbjct: 391 DIAEYYLEG----RKEYRTTGRSHHYVMLEKWFGMESILIEKERCKKRDLSDLLTFDSCF 450

Query: 545 WARLEEA----KDLLEIIKRDGDVRKLAPIWKSLENFERYARGLIERKEVSKDVIAKNSS 604
           WA +E++      L   +    DVR++  + + L  FE Y   +I ++EVS ++  + SS
Sbjct: 451 WAEVEDSLIVINQLNTTVGMRDDVREV--LTRKLVEFEGYVWEIITKREVSPEIFLEESS 510

Query: 605 YTLWAQELRALK 607
           +  W +E + +K
Sbjct: 511 FMKWWKEYKKIK 514

HSP 2 Score: 61.6 bits (148), Expect = 3.4e-08
Identity = 35/80 (43.75%), Postives = 47/80 (58.75%), Query Frame = 1

Query: 117 LVETITKVMERSKSIVITGHSLGGAAATLCTLWLLSFLHTKTHHHPILCITFGSPLIGNE 176
           L   + + ++  K ++ITG +LGG+ A+L TLWLL  +   T   P LCITFGSPLIG+ 
Sbjct: 126 LKSELLQTLKSEKPVIITGAALGGSVASLYTLWLLETIEP-TLKRP-LCITFGSPLIGDA 185

Query: 177 SLSRAIQRERWCGKFCHVVS 197
           SL + ++       F HVVS
Sbjct: 186 SLQQILENSVRNSCFLHVVS 203

BLAST of Csa4G496760 vs. TrEMBL
Match: A0A0A0L3S4_CUCSA (PAD4 OS=Cucumis sativus GN=Csa_4G496760 PE=4 SV=1)

HSP 1 Score: 1250.3 bits (3234), Expect = 0.0e+00
Identity = 609/609 (100.00%), Postives = 609/609 (100.00%), Query Frame = 1

Query: 1   MESEASTFESCHVMAALLGSTPLLLQSWEFCAAANAASPESFTTVVIDDVAYVGFSGVQV 60
           MESEASTFESCHVMAALLGSTPLLLQSWEFCAAANAASPESFTTVVIDDVAYVGFSGVQV
Sbjct: 1   MESEASTFESCHVMAALLGSTPLLLQSWEFCAAANAASPESFTTVVIDDVAYVGFSGVQV 60

Query: 61  LPRCGGGGRELVALDGEGVEAELFWPLNRHREELQEPAMADSGILKMFVDIYTHKNLVET 120
           LPRCGGGGRELVALDGEGVEAELFWPLNRHREELQEPAMADSGILKMFVDIYTHKNLVET
Sbjct: 61  LPRCGGGGRELVALDGEGVEAELFWPLNRHREELQEPAMADSGILKMFVDIYTHKNLVET 120

Query: 121 ITKVMERSKSIVITGHSLGGAAATLCTLWLLSFLHTKTHHHPILCITFGSPLIGNESLSR 180
           ITKVMERSKSIVITGHSLGGAAATLCTLWLLSFLHTKTHHHPILCITFGSPLIGNESLSR
Sbjct: 121 ITKVMERSKSIVITGHSLGGAAATLCTLWLLSFLHTKTHHHPILCITFGSPLIGNESLSR 180

Query: 181 AIQRERWCGKFCHVVSNHDIMPRLLSTPLSSLSPKLHILLRYWHLSMASPTFGKLATQLT 240
           AIQRERWCGKFCHVVSNHDIMPRLLSTPLSSLSPKLHILLRYWHLSMASPTFGKLATQLT
Sbjct: 181 AIQRERWCGKFCHVVSNHDIMPRLLSTPLSSLSPKLHILLRYWHLSMASPTFGKLATQLT 240

Query: 241 EREKEELFHIVLAHSNRISDLGEGTVQSQFWPFGNFFFCSEHGAICLDNAISVLKMLYLM 300
           EREKEELFHIVLAHSNRISDLGEGTVQSQFWPFGNFFFCSEHGAICLDNAISVLKMLYLM
Sbjct: 241 EREKEELFHIVLAHSNRISDLGEGTVQSQFWPFGNFFFCSEHGAICLDNAISVLKMLYLM 300

Query: 301 LKTSAPNLSIEDHLNYGYHVKKVGVQYMERKNFNSSCPPNSSYEAGLALALQSAGIPFQD 360
           LKTSAPNLSIEDHLNYGYHVKKVGVQYMERKNFNSSCPPNSSYEAGLALALQSAGIPFQD
Sbjct: 301 LKTSAPNLSIEDHLNYGYHVKKVGVQYMERKNFNSSCPPNSSYEAGLALALQSAGIPFQD 360

Query: 361 EVAQIAEHCLRTASRIGQTPNMNAAKLAISLSKITPYRAEIEWYKASCEEADNQLGYYDC 420
           EVAQIAEHCLRTASRIGQTPNMNAAKLAISLSKITPYRAEIEWYKASCEEADNQLGYYDC
Sbjct: 361 EVAQIAEHCLRTASRIGQTPNMNAAKLAISLSKITPYRAEIEWYKASCEEADNQLGYYDC 420

Query: 421 FKKEDASVRHDRVNMNRHKLATFWNRVINMWENNELPPDFNTRAKWVNASQFYKLLVEPL 480
           FKKEDASVRHDRVNMNRHKLATFWNRVINMWENNELPPDFNTRAKWVNASQFYKLLVEPL
Sbjct: 421 FKKEDASVRHDRVNMNRHKLATFWNRVINMWENNELPPDFNTRAKWVNASQFYKLLVEPL 480

Query: 481 DIAEYYHRDMHIVHGHYLKCGRERRYEIFDKWWRGREVTEEGNTQRMKYASLTQDSCFWA 540
           DIAEYYHRDMHIVHGHYLKCGRERRYEIFDKWWRGREVTEEGNTQRMKYASLTQDSCFWA
Sbjct: 481 DIAEYYHRDMHIVHGHYLKCGRERRYEIFDKWWRGREVTEEGNTQRMKYASLTQDSCFWA 540

Query: 541 RLEEAKDLLEIIKRDGDVRKLAPIWKSLENFERYARGLIERKEVSKDVIAKNSSYTLWAQ 600
           RLEEAKDLLEIIKRDGDVRKLAPIWKSLENFERYARGLIERKEVSKDVIAKNSSYTLWAQ
Sbjct: 541 RLEEAKDLLEIIKRDGDVRKLAPIWKSLENFERYARGLIERKEVSKDVIAKNSSYTLWAQ 600

Query: 601 ELRALKLNM 610
           ELRALKLNM
Sbjct: 601 ELRALKLNM 609

BLAST of Csa4G496760 vs. TrEMBL
Match: A0A0B2Q5X4_GLYSO (Lipase OS=Glycine soja GN=glysoja_003038 PE=4 SV=1)

HSP 1 Score: 627.1 bits (1616), Expect = 2.3e-176
Identity = 318/610 (52.13%), Postives = 420/610 (68.85%), Query Frame = 1

Query: 3   SEASTFESCHVMAALLGSTPLLLQSWEFCAAANAASPESFTTVVIDDVAYVGFSGVQVLP 62
           +E S FES  ++A+ + STPLL  SW  C  ANA    +F T  +    YV FSGV +  
Sbjct: 6   NETSPFESREMLASFVSSTPLLSDSWRLCTQANATPFLTFVTERVGASVYVAFSGVHMAG 65

Query: 63  RCGGGGRELVALDGEGVEAELFWPLNRHREELQEPAMADSGILKMFVDIYT--HKNLVET 122
                 R L  L   G    LF   +R  +E +EP M  +GIL +F  ++      ++E 
Sbjct: 66  ESDPNWRNLTPLYSIG-GLPLF--SSRRSKEWEEPVMVHAGILNLFFSLFNSFQNQMLEI 125

Query: 123 ITKVMERSKSIVITGHSLGGAAATLCTLWLLSFLHTKTHHHPILCITFGSPLIGNESLSR 182
           +    + +KS+VITGHS+GGA A+LCTLWLLS+L + +    ILCIT+G+PLIGNES S+
Sbjct: 126 VGN--KDTKSVVITGHSIGGATASLCTLWLLSYLQSISSSVSILCITYGAPLIGNESFSQ 185

Query: 183 AIQRERWCGKFCHVVSNHDIMPRLLSTPLSSLSPKLHILLRYWHLSMASPTFGKLATQLT 242
            I +ERW G FCHVVS HDIMPRLL  P++SLS +L+ LL++WHLSM SP FGKLA Q++
Sbjct: 186 TIFKERWGGNFCHVVSKHDIMPRLLFAPITSLSTQLNSLLQFWHLSMTSPDFGKLANQIS 245

Query: 243 EREKEELFHIVLAHSNRISDLGEGTVQSQFWPFGNFFFCSEHGAICLDNAISVLKMLYLM 302
           E+EK++LF  V+ +    +  GE +    F PFG++FF SE GA+C+D+  +++KM++LM
Sbjct: 246 EKEKDKLFTAVMDYLEAATQDGEKSAPILFHPFGSYFFVSEEGAVCVDSPSAIIKMMHLM 305

Query: 303 LKTSAPNLSIEDHLNYGYHVKKVGVQYMERKNFNSSCPPNSSYEAGLALALQSAGIPFQD 362
           L TS+P  SIEDHL YG +V K+  Q + + N      P+SSYEAGL LA+QS+GI  Q+
Sbjct: 306 LATSSPASSIEDHLKYGDYVNKMSAQTLYQSNSMQKNIPDSSYEAGLELAIQSSGIANQE 365

Query: 363 EVAQIAEHCLRTASRIGQTPNMNAAKLAISLSKITPYRAEIEWYKASCEEADNQLGYYDC 422
                A+ CL+T  R+G +P +NAA LA+SLSK+ PYRA+IEWYK  C+E D+Q+GYYD 
Sbjct: 366 PAITSAKECLKTTRRMGPSPTLNAASLAVSLSKVVPYRAQIEWYKTWCDEQDDQMGYYDS 425

Query: 423 FKKED--ASVRHDRVNMNRHKLATFWNRVINMWENNELPPDFNTRAKWVNASQFYKLLVE 482
           FK  D  +S R  ++N+NR KLA FWN VI+M E  ELP DF+ RAKWVN S FYKLLVE
Sbjct: 426 FKSRDSSSSKRDMKININRCKLARFWNNVIDMLERGELPHDFDKRAKWVNTSHFYKLLVE 485

Query: 483 PLDIAEYYHRDMHIVHGHYLKCGRERRYEIFDKWWRGREVT--EEGNTQRMKYASLTQDS 542
           PLDIAEYY + MH   GHY++ GRERRYEIFD+WW+ + VT   E N +R K+ASLTQDS
Sbjct: 486 PLDIAEYYGKGMHRTKGHYMQHGRERRYEIFDRWWKDKTVTTGREENKERSKFASLTQDS 545

Query: 543 CFWARLEEAKDLLEIIKRDGDVRKLAPIWKSLENFERYARGLIERKEVSKDVIAKNSSYT 602
           CFWAR+EEA+D L  ++ + D  KLA +W  +ENFE+YA  LIE KEVS DV+ KNSSY+
Sbjct: 546 CFWARVEEARDWLNCVRSERDTNKLALLWDKIENFEKYAIDLIENKEVSGDVLFKNSSYS 605

Query: 603 LWAQELRALK 607
           +W ++LR LK
Sbjct: 606 IWVEDLRELK 610

BLAST of Csa4G496760 vs. TrEMBL
Match: K7L476_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_08G002100 PE=4 SV=1)

HSP 1 Score: 627.1 bits (1616), Expect = 2.3e-176
Identity = 318/610 (52.13%), Postives = 420/610 (68.85%), Query Frame = 1

Query: 3   SEASTFESCHVMAALLGSTPLLLQSWEFCAAANAASPESFTTVVIDDVAYVGFSGVQVLP 62
           +E S FES  ++A+ + STPLL  SW  C  ANA    +F T  +    YV FSGV +  
Sbjct: 6   NETSPFESREMLASFVSSTPLLSDSWRLCTQANATPFLTFVTERVGASVYVAFSGVHMAG 65

Query: 63  RCGGGGRELVALDGEGVEAELFWPLNRHREELQEPAMADSGILKMFVDIYT--HKNLVET 122
                 R L  L   G    LF   +R  +E +EP M  +GIL +F  ++      ++E 
Sbjct: 66  ESDPNWRNLTPLYSIG-GLPLF--SSRRSKEWEEPVMVHAGILNLFFSLFNSFQNQMLEI 125

Query: 123 ITKVMERSKSIVITGHSLGGAAATLCTLWLLSFLHTKTHHHPILCITFGSPLIGNESLSR 182
           +    + +KS+VITGHS+GGA A+LCTLWLLS+L + +    ILCIT+G+PLIGNES S+
Sbjct: 126 VGN--KDTKSVVITGHSIGGATASLCTLWLLSYLQSISSSVSILCITYGAPLIGNESFSQ 185

Query: 183 AIQRERWCGKFCHVVSNHDIMPRLLSTPLSSLSPKLHILLRYWHLSMASPTFGKLATQLT 242
            I +ERW G FCHVVS HDIMPRLL  P++SLS +L+ LL++WHLSM SP FGKLA Q++
Sbjct: 186 TIFKERWGGNFCHVVSKHDIMPRLLFAPITSLSTQLNSLLQFWHLSMTSPDFGKLANQIS 245

Query: 243 EREKEELFHIVLAHSNRISDLGEGTVQSQFWPFGNFFFCSEHGAICLDNAISVLKMLYLM 302
           E+EK++LF  V+ +    +  GE +    F PFG++FF SE GA+C+D+  +++KM++LM
Sbjct: 246 EKEKDKLFTAVMDYLEAATQDGEKSAPILFHPFGSYFFVSEEGAVCVDSPSAIIKMMHLM 305

Query: 303 LKTSAPNLSIEDHLNYGYHVKKVGVQYMERKNFNSSCPPNSSYEAGLALALQSAGIPFQD 362
           L TS+P  SIEDHL YG +V K+  Q + + N      P+SSYEAGL LA+QS+GI  Q+
Sbjct: 306 LATSSPASSIEDHLKYGDYVNKMSAQTLYQSNSMQKNIPDSSYEAGLELAIQSSGIANQE 365

Query: 363 EVAQIAEHCLRTASRIGQTPNMNAAKLAISLSKITPYRAEIEWYKASCEEADNQLGYYDC 422
                A+ CL+T  R+G +P +NAA LA+SLSK+ PYRA+IEWYK  C+E D+Q+GYYD 
Sbjct: 366 PAITSAKECLKTTRRMGPSPTLNAASLAVSLSKVVPYRAQIEWYKTWCDEQDDQMGYYDS 425

Query: 423 FKKED--ASVRHDRVNMNRHKLATFWNRVINMWENNELPPDFNTRAKWVNASQFYKLLVE 482
           FK  D  +S R  ++N+NR KLA FWN VI+M E  ELP DF+ RAKWVN S FYKLLVE
Sbjct: 426 FKSRDSSSSKRDMKININRCKLARFWNNVIDMLERGELPHDFDKRAKWVNTSHFYKLLVE 485

Query: 483 PLDIAEYYHRDMHIVHGHYLKCGRERRYEIFDKWWRGREVT--EEGNTQRMKYASLTQDS 542
           PLDIAEYY + MH   GHY++ GRERRYEIFD+WW+ + VT   E N +R K+ASLTQDS
Sbjct: 486 PLDIAEYYGKGMHRTKGHYMQHGRERRYEIFDRWWKDKTVTTGREENKERSKFASLTQDS 545

Query: 543 CFWARLEEAKDLLEIIKRDGDVRKLAPIWKSLENFERYARGLIERKEVSKDVIAKNSSYT 602
           CFWAR+EEA+D L  ++ + D  KLA +W  +ENFE+YA  LIE KEVS DV+ KNSSY+
Sbjct: 546 CFWARVEEARDWLNCVRSERDTNKLALLWDKIENFEKYAIDLIENKEVSGDVLFKNSSYS 605

Query: 603 LWAQELRALK 607
           +W ++LR LK
Sbjct: 606 IWVEDLRELK 610

BLAST of Csa4G496760 vs. TrEMBL
Match: D2DF32_SOYBN (PAD4 OS=Glycine max PE=2 SV=1)

HSP 1 Score: 622.5 bits (1604), Expect = 5.6e-175
Identity = 316/610 (51.80%), Postives = 419/610 (68.69%), Query Frame = 1

Query: 3   SEASTFESCHVMAALLGSTPLLLQSWEFCAAANAASPESFTTVVIDDVAYVGFSGVQVLP 62
           +E S FES  ++A+ + STPLL  SW  C  ANA    +F T  +    YV FSGV +  
Sbjct: 6   NETSPFESREMLASFVSSTPLLSDSWRLCTQANATPFLTFVTERVGASVYVAFSGVHMAG 65

Query: 63  RCGGGGRELVALDGEGVEAELFWPLNRHREELQEPAMADSGILKMFVDIYT--HKNLVET 122
                 R L  L   G    LF   +R  +E +EP M  +GIL +F  ++      ++E 
Sbjct: 66  ESDPNWRNLTPLYSIG-GLPLF--SSRRSKEWEEPVMVHAGILNLFFSLFNSFQNQMLEI 125

Query: 123 ITKVMERSKSIVITGHSLGGAAATLCTLWLLSFLHTKTHHHPILCITFGSPLIGNESLSR 182
           +    + +KS+VITGHS+GGA A+LCTLWLLS+L + +    ILCIT+G+PLIGNES S+
Sbjct: 126 VGN--KDTKSVVITGHSIGGATASLCTLWLLSYLQSISSSVSILCITYGAPLIGNESFSQ 185

Query: 183 AIQRERWCGKFCHVVSNHDIMPRLLSTPLSSLSPKLHILLRYWHLSMASPTFGKLATQLT 242
            I +ERW G FCHVVS HDIMPRLL  P++SLS +L+ LL++WHLSM SP FGKLA Q++
Sbjct: 186 TIFKERWGGNFCHVVSKHDIMPRLLFAPITSLSTQLNSLLQFWHLSMTSPDFGKLANQIS 245

Query: 243 EREKEELFHIVLAHSNRISDLGEGTVQSQFWPFGNFFFCSEHGAICLDNAISVLKMLYLM 302
           E+EK++LF  V+ +    +  GE +    F PFG++FF SE GA+C+D+  +++KM++LM
Sbjct: 246 EKEKDKLFTAVMDYLEAATQDGEKSAPILFHPFGSYFFVSEEGAVCVDSPSAIIKMMHLM 305

Query: 303 LKTSAPNLSIEDHLNYGYHVKKVGVQYMERKNFNSSCPPNSSYEAGLALALQSAGIPFQD 362
           L TS+P  SIEDHL YG +V K+  Q + + N      P+SSYEAGL LA+QS+GI  Q+
Sbjct: 306 LATSSPASSIEDHLKYGDYVNKMSAQTLYQSNSMQKNIPDSSYEAGLELAIQSSGIANQE 365

Query: 363 EVAQIAEHCLRTASRIGQTPNMNAAKLAISLSKITPYRAEIEWYKASCEEADNQLGYYDC 422
                A+ CL+T  R+G +P +NAA LA+SLSK+ PYRA+IEWYK  C+E D+Q+GYYD 
Sbjct: 366 PAITSAKECLKTTRRMGPSPTLNAASLAVSLSKVVPYRAQIEWYKTWCDEQDDQMGYYDS 425

Query: 423 FKKED--ASVRHDRVNMNRHKLATFWNRVINMWENNELPPDFNTRAKWVNASQFYKLLVE 482
           FK  D  +S R  ++N+NR KLA FWN VI+M E  ELP DF+ RAKWVN S FYKLLVE
Sbjct: 426 FKSRDSPSSKRDMKININRCKLARFWNNVIDMLERGELPHDFDKRAKWVNTSHFYKLLVE 485

Query: 483 PLDIAEYYHRDMHIVHGHYLKCGRERRYEIFDKWWRGREVT--EEGNTQRMKYASLTQDS 542
           PLDIA+ Y + MH   GHY++ GRERRYEIFD+WW+   VT  +E N +R K+ASLTQDS
Sbjct: 486 PLDIADIYGKGMHRTKGHYMQHGRERRYEIFDRWWKDETVTTGKEENKERSKFASLTQDS 545

Query: 543 CFWARLEEAKDLLEIIKRDGDVRKLAPIWKSLENFERYARGLIERKEVSKDVIAKNSSYT 602
           CFWAR+EEA+D L  ++ + D  KLA +W  +ENFE+YA  LIE KEVS DV+ KNSSY+
Sbjct: 546 CFWARVEEARDWLNCVRSERDTNKLALLWDKIENFEKYAIDLIENKEVSGDVLFKNSSYS 605

Query: 603 LWAQELRALK 607
           +W ++LR LK
Sbjct: 606 IWVEDLRELK 610

BLAST of Csa4G496760 vs. TrEMBL
Match: V4T711_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10000576mg PE=4 SV=1)

HSP 1 Score: 610.5 bits (1573), Expect = 2.2e-171
Identity = 315/619 (50.89%), Postives = 415/619 (67.04%), Query Frame = 1

Query: 1   MESEASTFESCHVMAALLGSTPLLLQSWEFCA-AANAASP----ESFTTVVIDDVAYVGF 60
           ME EAS+FES  ++A  + S+PLL +SW  C     AASP    +SF    +  + YV F
Sbjct: 1   MEHEASSFESSEMLANHVASSPLLSESWRLCCDITTAASPSIPGQSFVMKQVGSIGYVAF 60

Query: 61  SGV-----QVLPRCGGGGRELVALDGEGVEAELFWPLNRH-REELQEPAMADSGILKMFV 120
           S +       +  C G    LVALD +      F PLN+   E  +EP +  +G L++F 
Sbjct: 61  SSIISEAEAGICCCNGN---LVALDDQ-----FFSPLNKQINEGEEEPVLVHAGFLRLFF 120

Query: 121 DIYTHKNLVETITKVMERSKSIVITGHSLGGAAATLCTLWLLSFLH-TKTHHHPILCITF 180
            IY   +    + +++++SKSIVITGHS+    A+L TLWLLS L  + +   PILCITF
Sbjct: 121 SIYASPSFQTQMMEIIQKSKSIVITGHSVRATTASLSTLWLLSHLQKSNSPSLPILCITF 180

Query: 181 GSPLIGNESLSRAIQRERWCGKFCHVVSNHDIMPRLLSTPLSSLSPKLHILLRYWHLSMA 240
           GSPL+GN SLSRAI RERW G FCHVVS HDIMPRLL  P      +L  LL +WHLSM 
Sbjct: 181 GSPLLGNASLSRAILRERWDGNFCHVVSKHDIMPRLLFVPPLHFINQLKFLLNFWHLSMT 240

Query: 241 SPTFGKLATQLTEREKEELFHIVLAHSNRISDLGE-GTVQSQFWPFGNFFFCSEHGAICL 300
           SP F  LATQL   +K E+F  V+A    ++   E G+    FWPFG++FFCSE GAIC+
Sbjct: 241 SPQFQTLATQLNNEQKAEIFRSVMACLEVLAQAEEAGSETRAFWPFGSYFFCSEEGAICM 300

Query: 301 DNAISVLKMLYLMLKTSAPNLSIEDHLNYGYHVKKVGVQYMERKNFNSSCPPNSSYEAGL 360
           +NA SV+KM++LML T +P  SIEDHL YG ++ K+  Q+++++N      P S YEAG+
Sbjct: 301 ENATSVIKMMHLMLMTGSPCASIEDHLKYGDYIGKISYQFLKQRNSVDGDIPESCYEAGV 360

Query: 361 ALALQSAGIPFQDEVAQIAEHCLRTASRIGQTPNMNAAKLAISLSKITPYRAEIEWYKAS 420
           ALALQS+ I  Q+ V+  A+ CL+ A R+G+TPN+N A LAI LS I PYRA+IEWYKA 
Sbjct: 361 ALALQSSAISSQEPVSTAAKDCLKMARRMGRTPNLNVADLAIKLSMINPYRAQIEWYKAC 420

Query: 421 CEEADNQLGYYDCFKKEDASVRHDRVNMNRHKLATFWNRVINMWENNELPPDFNTRAKWV 480
           C+++D Q+GYYD FK    S R  +VNMNR  L  FW+ VI+  ENN+LP DF+ R+KWV
Sbjct: 421 CDDSDEQMGYYDSFKLRGVSKRDSKVNMNRIMLGKFWDGVIDKLENNDLPHDFHRRSKWV 480

Query: 481 NASQFYKLLVEPLDIAEYYHRDMHIVHGHYLKCGRERRYEIFDKWWRGREVTEEGNTQRM 540
           NASQFY LLVEPLDIA+YY    H   GHY+K GR RRYEIF++WW+ R V+ E N +R 
Sbjct: 481 NASQFYMLLVEPLDIADYYRNGHHRSKGHYVKHGRPRRYEIFERWWKERRVSNEENNKRS 540

Query: 541 KYASLTQDSCFWARLEEAKDLLEIIKRDGDVRKLAPIWKSLENFERYARGLIERKEVSKD 600
           + ASLTQDSCFWA LEEA++ L+ ++ + D  KL  +W+++ NFE++A GL+E K+VSKD
Sbjct: 541 RLASLTQDSCFWAELEEARECLDNVRSERDPNKLDLLWQNINNFEKHAVGLVESKQVSKD 600

Query: 601 VIAKNSSYTLWAQELRALK 607
           V+A+NSSY  W  +LR L+
Sbjct: 601 VLARNSSYVSWVDDLRELR 611

BLAST of Csa4G496760 vs. TAIR10
Match: AT3G52430.1 (AT3G52430.1 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 305.1 bits (780), Expect = 9.9e-83
Identity = 173/426 (40.61%), Postives = 248/426 (58.22%), Query Frame = 1

Query: 189 GKFCHVVSNHDIMPRLLS-TPLSSLSPKLHILLRYWHLSMASPTFGK--LATQLTEREKE 248
           GK   V++ H     L + T L  LS       R + ++  SP  G   L+T ++     
Sbjct: 108 GKKLVVITGHSTGGALAAFTALWLLSQSSPPSFRVFCITFGSPLLGNQSLSTSISRSRLA 167

Query: 249 ELF-HIVLAHSNRISDLGEGTVQSQFWPFGNFFFCSEHGAICLDNAISVLKMLYLMLKTS 308
             F H+V  H     DL   +   QFWPFG + FCS+ G +CLDNA SV  M  ++  T+
Sbjct: 168 HNFCHVVSIH-----DLVPRSSNEQFWPFGTYLFCSDKGGVCLDNAGSVRLMFNILNTTA 227

Query: 309 APNLSIEDHLNYGYHVKKVGVQYMERKNFNSSCPPNSSYEAGLALALQSAGIPFQDEVAQ 368
             N   E+H  YG++V  +   +++ ++F     P++SY+AG+ALA+++ G    D    
Sbjct: 228 TQNT--EEHQRYGHYVFTLSHMFLKSRSFLGGSIPDNSYQAGVALAVEALGFSNDDTSGV 287

Query: 369 IAEHCLRTASRIGQTPNMNAAKLAISLSKITPYRAEIEWYKASCEEADNQLGYYDCFKKE 428
           + + C+ TA+RI + P + +A+LA  L+ + P R EI+WYK  C+ ++ QLGYYD FK+ 
Sbjct: 288 LVKECIETATRIVRAPILRSAELANELASVLPARLEIQWYKDRCDASEEQLGYYDFFKRY 347

Query: 429 DASVRHDRVNMNRHKLATFWNRVINMWENNELPPDFNTRAKWVNASQFYKLLVEPLDIAE 488
               R  +VNM+R +LA FW+ VI M E NELP DF+   KW+ ASQFY+LL EPLDIA 
Sbjct: 348 SLK-RDFKVNMSRIRLAKFWDTVIKMVETNELPFDFHLGKKWIYASQFYQLLAEPLDIAN 407

Query: 489 YYHRDMHIVHGHYLKCGRERRYEIFDKWWRGREVTEEGNTQRMKYASLTQDSCFWARLEE 548
           +Y        GHYL+  R +RYE+ DKW +G +V EE    R +YAS TQD+CFWA+LE+
Sbjct: 408 FYKNRDIKTGGHYLEGNRPKRYEVIDKWQKGVKVPEE--CVRSRYASTTQDTCFWAKLEQ 467

Query: 549 AKDLL-EIIKRDGDVRKLAPIWKSLENFERYARGLIERKEVSKDVIAKNSSYTLWAQELR 608
           AK+ L E  K   D ++ + + + +  FE YA  L+ +KEVS DV AKNSSY++W   L+
Sbjct: 468 AKEWLDEARKESSDPQRRSLLREKIVPFESYANTLVTKKEVSLDVKAKNSSYSVWEANLK 523

Query: 609 ALKLNM 610
             K  M
Sbjct: 528 EFKCKM 523

HSP 2 Score: 110.9 bits (276), Expect = 2.8e-24
Identity = 76/200 (38.00%), Postives = 103/200 (51.50%), Query Frame = 1

Query: 8   FESCHVMAALLGSTPLLLQSWEFCAAANAASPESFTTVVIDDVA---YVGFSGVQVLPRC 67
           FE+  + A+++ STPL   SW  C  AN        ++ I D+A   YV    V ++   
Sbjct: 6   FETSELQASVMISTPLFTDSWSSCNTANCNG-----SIKIHDIAGITYVAIPAVSMI--- 65

Query: 68  GGGGRELVALDGEGVEAELFWPLNRHREELQEPAMADSGILKMFVDIYTHKNL-VETITK 127
                +L  L G  V  ++ +P     E L    M D+ ILK+F+ +   + L +E + K
Sbjct: 66  -----QLGNLVGLPVTGDVLFPGLSSDEPLP---MVDAAILKLFLQLKIKEGLELELLGK 125

Query: 128 VMERSKSIVITGHSLGGAAATLCTLWLLSFLHTKTHHHPILCITFGSPLIGNESLSRAIQ 187
                K +VITGHS GGA A    LWLLS   +      + CITFGSPL+GN+SLS +I 
Sbjct: 126 -----KLVVITGHSTGGALAAFTALWLLS--QSSPPSFRVFCITFGSPLLGNQSLSTSIS 182

Query: 188 RERWCGKFCHVVSNHDIMPR 204
           R R    FCHVVS HD++PR
Sbjct: 186 RSRLAHNFCHVVSIHDLVPR 182

BLAST of Csa4G496760 vs. TAIR10
Match: AT3G48080.1 (AT3G48080.1 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 149.8 bits (377), Expect = 5.3e-36
Identity = 127/521 (24.38%), Postives = 225/521 (43.19%), Query Frame = 1

Query: 120 TITKVMERSKSIVITGHSLGGAAATLCTLWLLS--FLHTKTHHHPILCITFGSPLIGNES 179
           ++   ++R + +V TGHS GGA A L T+W L   F+          C+TFG+PL+G+  
Sbjct: 106 SVQSAVDRKQQVVFTGHSFGGATAILATVWYLETYFIRDAYAAPEPRCVTFGAPLVGDYI 165

Query: 180 LSRAIQRERWCGKFCHVVSNHDIMPRLLSTPLSSLSPKLHILLRYWHLSMAS-PTFGKLA 239
              A+ RE W   F + V+  DI+PR++    +++   L  +L     + A      ++ 
Sbjct: 166 FKHALGRENWSRFFVNFVTRFDIVPRIMLARKTTIEQTLSYVLGKLDSTRAPIHESDQVI 225

Query: 240 TQLTEREKEELFHIVLAHSNRISDLGEGTVQ--------SQFWPFGNFFFCSEHGAICLD 299
           T+   R   + + +      ++   GE  ++        S + P G F F ++   + ++
Sbjct: 226 TEFYTRVMRDTYTVASKAVCQLIGNGEAFLETLSSFYELSPYRPVGTFVFSTQKRLVVVN 285

Query: 300 NAISVLKMLYLMLKTS-------APNLSIEDHLNYGYHVKKVGVQYMERKNFNSSCPPNS 359
           N+ ++L+ML+   +++        P LSI DH  Y   V+ +G++ +   + ++   P  
Sbjct: 286 NSDAILQMLFYTCQSNDEQELSVIPFLSIRDHHGYEELVQSIGIKLLNHLDLHN---PLL 345

Query: 360 SYEAGLALALQSAGIPFQDEVAQIAEHCLRTASRIGQTPNMNAAKLAISLSKITPYRAEI 419
             E  +  AL   G+  +      A  C+  A    +    N  K+     +I      I
Sbjct: 346 DGENSIGSALDDLGMSTR------ARQCIHAALEAEKQRVENQKKIETKRDQIVERLTWI 405

Query: 420 -EWYKASCEEADNQLGYYDCFKKEDASVRHD-RVNMNRHKLATFWNRVINMWENNELPPD 479
            E YK  C+   N  GYYD FK  D++  +D + N+ R +LA  ++ V+ + +  +LP  
Sbjct: 406 VEVYKPKCQAHKN--GYYDSFK--DSNEENDFKANVKRVELAGIFDEVLGLVKKGQLPDG 465

Query: 480 FNTRAKWVNASQFYKLLVEPLDIAEYYHRDMHIVHGHYLKCGRERRYEIFDKWWRGREVT 539
           F     W+N +  Y+ L+EPLDI+ Y+ +  +   G Y+  GR  RY+   + +    + 
Sbjct: 466 FEGSRGWINLATQYRRLIEPLDISNYHGQLKNEDTGPYMLHGRPSRYKYAQRGYEHDILK 525

Query: 540 EEGNTQRMKYAS--------LTQD-------------SCFWARLEEAKDLLEIIKRDGDV 599
             G   +  + S        L QD             SCFWA +EE K            
Sbjct: 526 PTGMIAKDVFWSKVNGLNLGLQQDIQEILKNSGSECGSCFWAEVEELKG----------- 585

BLAST of Csa4G496760 vs. TAIR10
Match: AT3G48090.1 (AT3G48090.1 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 141.4 bits (355), Expect = 1.9e-33
Identity = 133/502 (26.49%), Postives = 215/502 (42.83%), Query Frame = 1

Query: 98  AMADSGILKMFVDIYTHKNLVETITKVMERS-KSIVITGHSLGGAAATLCTLWLLS--FL 157
           A  +   LK    I   +   +   ++  RS K IV TGHS GGA A L T+W L   F+
Sbjct: 83  ATVNEAFLKNLEAIIDPRTSFQASVEMAVRSRKQIVFTGHSSGGATAILATVWYLEKYFI 142

Query: 158 HTKTHHHPILCITFGSPLIGNESLSRAIQRERWCGKFCHVVSNHDIMPRLLSTPLSSLSP 217
                +    C+TFG+PL+G+   S A+ RE+W   F + VS  DI+PR++    +S+  
Sbjct: 143 RNPNVYLEPRCVTFGAPLVGDSIFSHALGREKWSRFFVNFVSRFDIVPRIMLARKASVEE 202

Query: 218 KL-HILLRYWHLSMASPTFGKLATQLTEREKEELFHIVLAHSNRISD------------- 277
            L H+L      +   P   K + Q +E+   E +  V+  ++ +++             
Sbjct: 203 TLPHVL------AQLDPR--KSSVQESEQRITEFYTRVMRDTSTVANQAVCELTGSAEAF 262

Query: 278 ---LGEGTVQSQFWPFGNFFFCSEHGAICLDNAISVLKMLYLMLKTS-------APNLSI 337
              L      S + P G F F +E   + ++N+ ++L+ML+   + S        P  SI
Sbjct: 263 LETLSSFLELSPYRPAGTFVFSTEKRLVAVNNSDAILQMLFYTSQASDEQEWSLIPFRSI 322

Query: 338 EDHLNYGYHVKKVGVQYMERKNFNSSCPPNSSYEAGLALALQSAGIPFQDEVAQIAEHCL 397
            DH +Y   V+ +G     +K FN     NS       L + + G  +    A + E   
Sbjct: 323 RDHHSYEELVQSMG-----KKLFNHLDGENSIESTLNDLGVSTRGRQYVQ--AALEEEKK 382

Query: 398 RTASRIGQTPNMNAAKLAISLSKITPYRAEIEW----YKASCEEADNQLGYYDCFKKEDA 457
           R  ++          K  I + +   +  ++ W    YK  C+   N  GYYD FK  + 
Sbjct: 383 RVENQ----------KKIIQVIEQERFLKKLAWIEDEYKPKCQAHKN--GYYDSFKVSNE 442

Query: 458 SVRHDRVNMNRHKLATFWNRVINMWENNELPPDFNTRAKWVNASQFYKLLVEPLDIAEYY 517
                + N+ R +LA  ++ V+ + +  +LP +F     W+  +  Y+ LVEPLDIA Y+
Sbjct: 443 E-NDFKANVKRAELAGVFDEVLGLMKKCQLPDEFEGDIDWIKLATRYRRLVEPLDIANYH 502

Query: 518 HRDMHIVHGHYLKCGR-------ERRYEIF----------DKWWR-----GREVTEEGNT 547
               +   G Y+K GR       +R YE +          D +W         +  E   
Sbjct: 503 RHLKNEDTGPYMKRGRPTRYIYAQRGYEHYILKPNGMIAEDVFWNKVNGLNLGLQLEEIQ 556

BLAST of Csa4G496760 vs. TAIR10
Match: AT5G14930.2 (AT5G14930.2 senescence-associated gene 101)

HSP 1 Score: 100.9 bits (250), Expect = 2.8e-21
Identity = 73/252 (28.97%), Postives = 116/252 (46.03%), Query Frame = 1

Query: 365 IAEHCLRTASRIGQTPNMNAAKLAISLSKITPYRAEIEWYKASCEEADNQLGYYDCFKKE 424
           I E  ++   +  +  N+    +   L+ +    A IEWYK  C+E   ++GYYD FK +
Sbjct: 271 IPEDVIKGIEKRAEMKNLRFDMMFKKLNDMKISMAYIEWYKKKCKEV--KIGYYDRFKTQ 330

Query: 425 DA--SVRHDRVNMNRHK--LATFWNRVINMWENNELPPDFNTRAKWVNASQFYKLLVEPL 484
            A  S   D    N HK  L  FW  V+   E          + +++ +   Y+ ++EPL
Sbjct: 331 LAFPSKEFDINIKNHHKSELNRFWKSVVEEVERRPQSDASILKRRFLFSGNNYRRMIEPL 390

Query: 485 DIAEYYHRDMHIVHGHYLKCGRERRYEIFDKWWRGREVTEEGNTQRMKYAS--LTQDSCF 544
           DIAEYY          Y   GR   Y + +KW+    +  E    + +  S  LT DSCF
Sbjct: 391 DIAEYYLEG----RKEYRTTGRSHHYVMLEKWFGMESILIEKERCKKRDLSDLLTFDSCF 450

Query: 545 WARLEEA----KDLLEIIKRDGDVRKLAPIWKSLENFERYARGLIERKEVSKDVIAKNSS 604
           WA +E++      L   +    DVR++  + + L  FE Y   +I ++EVS ++  + SS
Sbjct: 451 WAEVEDSLIVINQLNTTVGMRDDVREV--LTRKLVEFEGYVWEIITKREVSPEIFLEESS 510

Query: 605 YTLWAQELRALK 607
           +  W +E + +K
Sbjct: 511 FMKWWKEYKKIK 514

HSP 2 Score: 61.6 bits (148), Expect = 1.9e-09
Identity = 35/80 (43.75%), Postives = 47/80 (58.75%), Query Frame = 1

Query: 117 LVETITKVMERSKSIVITGHSLGGAAATLCTLWLLSFLHTKTHHHPILCITFGSPLIGNE 176
           L   + + ++  K ++ITG +LGG+ A+L TLWLL  +   T   P LCITFGSPLIG+ 
Sbjct: 126 LKSELLQTLKSEKPVIITGAALGGSVASLYTLWLLETIEP-TLKRP-LCITFGSPLIGDA 185

Query: 177 SLSRAIQRERWCGKFCHVVS 197
           SL + ++       F HVVS
Sbjct: 186 SLQQILENSVRNSCFLHVVS 203

BLAST of Csa4G496760 vs. TAIR10
Match: AT3G07400.1 (AT3G07400.1 lipase class 3 family protein)

HSP 1 Score: 57.0 bits (136), Expect = 4.7e-08
Identity = 45/161 (27.95%), Postives = 68/161 (42.24%), Query Frame = 1

Query: 118 VETITKVMERSKSIVITGHSLGGAAATLCTLWLLSFLHTKT----HHHPILCITFGSPLI 177
           +E      ++ + +V+ GHSLGGA A L TL +L  +   +     +  + CITF  P +
Sbjct: 216 LELYRLAQKKKRKLVLCGHSLGGAVAALATLAILRVVAASSKRGNENIHVKCITFSQPPV 275

Query: 178 GNESLSRAIQRERWCGKFCHVVSNHDIMPRLLSTPLSSLSPKLHILLRYWHLSMA---SP 237
           GN +L   +  + W   F       D++PR+       LSP          +SMA     
Sbjct: 276 GNAALRDYVHEKGWHHYFKSYCIPEDLVPRI-------LSPAYFHHYNEQRISMAGETEA 335

Query: 238 TFGKLATQLTEREKEELFHIVLAHSNRISDLGEGTVQSQFW 272
           T G+  T   E+ K +       H   +  +G G VQ+ FW
Sbjct: 336 TNGQGVTSEAEKRKTK------EHEQLV--IGVGPVQNSFW 361

BLAST of Csa4G496760 vs. NCBI nr
Match: gi|778694921|ref|XP_011653897.1| (PREDICTED: lipase-like PAD4 [Cucumis sativus])

HSP 1 Score: 1250.3 bits (3234), Expect = 0.0e+00
Identity = 609/609 (100.00%), Postives = 609/609 (100.00%), Query Frame = 1

Query: 1   MESEASTFESCHVMAALLGSTPLLLQSWEFCAAANAASPESFTTVVIDDVAYVGFSGVQV 60
           MESEASTFESCHVMAALLGSTPLLLQSWEFCAAANAASPESFTTVVIDDVAYVGFSGVQV
Sbjct: 1   MESEASTFESCHVMAALLGSTPLLLQSWEFCAAANAASPESFTTVVIDDVAYVGFSGVQV 60

Query: 61  LPRCGGGGRELVALDGEGVEAELFWPLNRHREELQEPAMADSGILKMFVDIYTHKNLVET 120
           LPRCGGGGRELVALDGEGVEAELFWPLNRHREELQEPAMADSGILKMFVDIYTHKNLVET
Sbjct: 61  LPRCGGGGRELVALDGEGVEAELFWPLNRHREELQEPAMADSGILKMFVDIYTHKNLVET 120

Query: 121 ITKVMERSKSIVITGHSLGGAAATLCTLWLLSFLHTKTHHHPILCITFGSPLIGNESLSR 180
           ITKVMERSKSIVITGHSLGGAAATLCTLWLLSFLHTKTHHHPILCITFGSPLIGNESLSR
Sbjct: 121 ITKVMERSKSIVITGHSLGGAAATLCTLWLLSFLHTKTHHHPILCITFGSPLIGNESLSR 180

Query: 181 AIQRERWCGKFCHVVSNHDIMPRLLSTPLSSLSPKLHILLRYWHLSMASPTFGKLATQLT 240
           AIQRERWCGKFCHVVSNHDIMPRLLSTPLSSLSPKLHILLRYWHLSMASPTFGKLATQLT
Sbjct: 181 AIQRERWCGKFCHVVSNHDIMPRLLSTPLSSLSPKLHILLRYWHLSMASPTFGKLATQLT 240

Query: 241 EREKEELFHIVLAHSNRISDLGEGTVQSQFWPFGNFFFCSEHGAICLDNAISVLKMLYLM 300
           EREKEELFHIVLAHSNRISDLGEGTVQSQFWPFGNFFFCSEHGAICLDNAISVLKMLYLM
Sbjct: 241 EREKEELFHIVLAHSNRISDLGEGTVQSQFWPFGNFFFCSEHGAICLDNAISVLKMLYLM 300

Query: 301 LKTSAPNLSIEDHLNYGYHVKKVGVQYMERKNFNSSCPPNSSYEAGLALALQSAGIPFQD 360
           LKTSAPNLSIEDHLNYGYHVKKVGVQYMERKNFNSSCPPNSSYEAGLALALQSAGIPFQD
Sbjct: 301 LKTSAPNLSIEDHLNYGYHVKKVGVQYMERKNFNSSCPPNSSYEAGLALALQSAGIPFQD 360

Query: 361 EVAQIAEHCLRTASRIGQTPNMNAAKLAISLSKITPYRAEIEWYKASCEEADNQLGYYDC 420
           EVAQIAEHCLRTASRIGQTPNMNAAKLAISLSKITPYRAEIEWYKASCEEADNQLGYYDC
Sbjct: 361 EVAQIAEHCLRTASRIGQTPNMNAAKLAISLSKITPYRAEIEWYKASCEEADNQLGYYDC 420

Query: 421 FKKEDASVRHDRVNMNRHKLATFWNRVINMWENNELPPDFNTRAKWVNASQFYKLLVEPL 480
           FKKEDASVRHDRVNMNRHKLATFWNRVINMWENNELPPDFNTRAKWVNASQFYKLLVEPL
Sbjct: 421 FKKEDASVRHDRVNMNRHKLATFWNRVINMWENNELPPDFNTRAKWVNASQFYKLLVEPL 480

Query: 481 DIAEYYHRDMHIVHGHYLKCGRERRYEIFDKWWRGREVTEEGNTQRMKYASLTQDSCFWA 540
           DIAEYYHRDMHIVHGHYLKCGRERRYEIFDKWWRGREVTEEGNTQRMKYASLTQDSCFWA
Sbjct: 481 DIAEYYHRDMHIVHGHYLKCGRERRYEIFDKWWRGREVTEEGNTQRMKYASLTQDSCFWA 540

Query: 541 RLEEAKDLLEIIKRDGDVRKLAPIWKSLENFERYARGLIERKEVSKDVIAKNSSYTLWAQ 600
           RLEEAKDLLEIIKRDGDVRKLAPIWKSLENFERYARGLIERKEVSKDVIAKNSSYTLWAQ
Sbjct: 541 RLEEAKDLLEIIKRDGDVRKLAPIWKSLENFERYARGLIERKEVSKDVIAKNSSYTLWAQ 600

Query: 601 ELRALKLNM 610
           ELRALKLNM
Sbjct: 601 ELRALKLNM 609

BLAST of Csa4G496760 vs. NCBI nr
Match: gi|659083007|ref|XP_008442139.1| (PREDICTED: senescence-associated carboxylesterase 101 [Cucumis melo])

HSP 1 Score: 1164.4 bits (3011), Expect = 0.0e+00
Identity = 573/610 (93.93%), Postives = 584/610 (95.74%), Query Frame = 1

Query: 1   MESEASTFESCHVMAALLGSTPLLLQSWEFCAAANAASPESFTTVVIDDVAYVGFSGVQV 60
           MESEASTFESCHVMAALLGSTPLLLQSWEFCAAANAASPESFTT+VIDDVAYV FSGVQV
Sbjct: 1   MESEASTFESCHVMAALLGSTPLLLQSWEFCAAANAASPESFTTLVIDDVAYVAFSGVQV 60

Query: 61  LPRCGGGG-RELVALDGEGVEAELFWPLNRHREELQEPAMADSGILKMFVDIYTHKNLVE 120
           LP CGGG  RELVALDGEGVE ELFWPL RHREELQEPAMADSGILKMFVDIY HKNLVE
Sbjct: 61  LPGCGGGRVRELVALDGEGVEGELFWPLKRHREELQEPAMADSGILKMFVDIYAHKNLVE 120

Query: 121 TITKVMERSKSIVITGHSLGGAAATLCTLWLLSFLHTKTHHHPILCITFGSPLIGNESLS 180
           T+TKVME+SKSIVITGHSLGGAAATLCTLWLLSF HTKTHHHPILCITFGSPLIGNESLS
Sbjct: 121 TMTKVMEKSKSIVITGHSLGGAAATLCTLWLLSFFHTKTHHHPILCITFGSPLIGNESLS 180

Query: 181 RAIQRERWCGKFCHVVSNHDIMPRLLSTPLSSLSPKLHILLRYWHLSMASPTFGKLATQL 240
           RAIQRERWCGKFCHVVSNHDIMPRLLSTPL SLSPKLHI+ RYWHLSMASP FGKLATQL
Sbjct: 181 RAIQRERWCGKFCHVVSNHDIMPRLLSTPLPSLSPKLHIIFRYWHLSMASPQFGKLATQL 240

Query: 241 TEREKEELFHIVLAHSNRISDLGEGTVQSQFWPFGNFFFCSEHGAICLDNAISVLKMLYL 300
           TEREKEELF+IVLAHSN I DLGEG+VQSQFWPFGNFFFCSEHGAICLDNAISVLKML L
Sbjct: 241 TEREKEELFYIVLAHSNTIFDLGEGSVQSQFWPFGNFFFCSEHGAICLDNAISVLKMLDL 300

Query: 301 MLKTSAPNLSIEDHLNYGYHVKKVGVQYMERKNFNSSCPPNSSYEAGLALALQSAGIPFQ 360
           MLKTSAPNLSIEDHLNYG HVKKVGVQYMERKN NS C PNSSYEAGLALAL+SAGIPFQ
Sbjct: 301 MLKTSAPNLSIEDHLNYGDHVKKVGVQYMERKNLNSLCLPNSSYEAGLALALESAGIPFQ 360

Query: 361 DEVAQIAEHCLRTASRIGQTPNMNAAKLAISLSKITPYRAEIEWYKASCEEADNQLGYYD 420
           DEVA +AEH LRTASRIGQTPNMNAAKLAISLSKITPYRAEIEWYK SC+EADNQLGYYD
Sbjct: 361 DEVAHMAEHSLRTASRIGQTPNMNAAKLAISLSKITPYRAEIEWYKTSCDEADNQLGYYD 420

Query: 421 CFKKEDASVRHDRVNMNRHKLATFWNRVINMWENNELPPDFNTRAKWVNASQFYKLLVEP 480
           CFKKEDASVRHDRVNMNRHKLATFWNRVI+MWENNELPPDFN RAKWVNASQFYKLLVEP
Sbjct: 421 CFKKEDASVRHDRVNMNRHKLATFWNRVIDMWENNELPPDFNMRAKWVNASQFYKLLVEP 480

Query: 481 LDIAEYYHRDMHIVHGHYLKCGRERRYEIFDKWWRGREVTEEGNTQRMKYASLTQDSCFW 540
           LDIAEYY RD HIVHGHYLKCGRERRYEIFDKWWRGREVTEEGNT RMKYASLTQDSCFW
Sbjct: 481 LDIAEYYRRDTHIVHGHYLKCGRERRYEIFDKWWRGREVTEEGNTPRMKYASLTQDSCFW 540

Query: 541 ARLEEAKDLLEIIKRDGDVRKLAPIWKSLENFERYARGLIERKEVSKDVIAKNSSYTLWA 600
           ARLEEAKDLLEIIKR GDVRKLAPIWKSLENFERYARGLIERKEVS+DVIAKNSSYTLWA
Sbjct: 541 ARLEEAKDLLEIIKR-GDVRKLAPIWKSLENFERYARGLIERKEVSEDVIAKNSSYTLWA 600

Query: 601 QELRALKLNM 610
           QELRALKLNM
Sbjct: 601 QELRALKLNM 609

BLAST of Csa4G496760 vs. NCBI nr
Match: gi|1009172596|ref|XP_015867358.1| (PREDICTED: lipase-like PAD4 [Ziziphus jujuba])

HSP 1 Score: 667.9 bits (1722), Expect = 1.7e-188
Identity = 340/613 (55.46%), Postives = 437/613 (71.29%), Query Frame = 1

Query: 1   MESEASTFESCHVMAALLGSTPLLLQSWEFCAAANAASPESFTTVVIDDVAYVGFSGVQV 60
           ME+EAS FE+  +++  L STPLL ++W  C+ AN  +P SF T +I DV YV FSG+Q+
Sbjct: 1   MEAEASPFETSEMLSTFLASTPLLSEAWRLCSLANTTAPMSFVTELIGDVGYVAFSGIQM 60

Query: 61  LPRCGGGG-RELVALDGEGVEAEL-FWPLNRHREELQEPAMADSGILKMFVDIYTHKNLV 120
           +        RE+V L+  G   E  F PL   R E +EP M   G+LK+F   +  +N  
Sbjct: 61  VDVSEPASCREMVPLESAGGLIENPFHPLLSARNEDEEPLMVHGGLLKLF---FGCQNFR 120

Query: 121 ETITKVMERSKSIVITGHSLGGAAATLCTLWLLSFLHTKTHHHPILCITFGSPLIGNESL 180
           + +  V+++SKS+V TGHSLGG+ A+L  LWLL +LH+ +   PI CITFGSPL+GNES 
Sbjct: 121 DQVLAVLQKSKSMVFTGHSLGGSIASLYALWLLCYLHSSSSSIPIFCITFGSPLLGNESF 180

Query: 181 SRAIQRERWCGKFCHVVSNHDIMPRLLSTPLSSLSPKLHILLRYWHLSMASPTFGKLA-- 240
           SRAI RERW G FCHVVS HDIMPRLL  PL  L+ +LH+LL +WHL+MAS   G  A  
Sbjct: 181 SRAILRERWGGNFCHVVSKHDIMPRLLFAPL--LTHQLHLLLNHWHLAMASQQIGNPAGV 240

Query: 241 TQLTEREKEELFHIVLAHSNRISDLG-EGTVQSQFWPFGNFFFCSEHGAICLDNAISVLK 300
            Q+ + +K      VLA+  R S L  EG  +  FWPFG++ FCS+ G ICL+NA+SV+K
Sbjct: 241 LQVPDEDKARFLRFVLAYLERSSQLAVEGERREMFWPFGSYLFCSKEGGICLENAVSVIK 300

Query: 301 MLYLMLKTSAPNLSIEDHLNYGYHVKKVGVQYMERKNF-NSSCPPNSSYEAGLALALQSA 360
           M++LM+ T + +  I DHLNYG ++     Q+++R+NF      P SSYEAGLALALQS+
Sbjct: 301 MMHLMVATVSLDECIMDHLNYGDYIGNFSSQFLKRRNFMQGGLGPCSSYEAGLALALQSS 360

Query: 361 GIPFQDEVAQIAEHCLRTASRIGQTPNMNAAKLAISLSKITPYRAEIEWYKASCEEADNQ 420
           GI +Q+  A  A+ CL+ A R GQ PN+N A LA+SLSKITPYRAE+EWYKA+C+E+D+Q
Sbjct: 361 GIAWQEPAAIPAKECLKMARRSGQKPNLNGANLAVSLSKITPYRAELEWYKATCDESDDQ 420

Query: 421 LGYYDCFKKEDASVRHDRVNMNRHKLATFWNRVINMWENNELPPDFNTRAKWVNASQFYK 480
           +GYYD FK+  +S R  RVNMNRHKLA+FWN+VI+M ENNELP DF+ RAKWVNAS FYK
Sbjct: 421 MGYYDSFKQSRSSKRGHRVNMNRHKLASFWNKVISMLENNELPYDFHKRAKWVNASHFYK 480

Query: 481 LLVEPLDIAEYYHRDMHIVHGHYLKCGRERRYEIFDKWWRGREVTE-EGNTQRMKYASLT 540
           LLVEPLDIAEYY    H V GHYL+ GRERRY+IFDKWW  + V   EGN +R K+ASLT
Sbjct: 481 LLVEPLDIAEYYRWGTHKVKGHYLEHGRERRYQIFDKWWNEKTVVNGEGNNKRSKFASLT 540

Query: 541 QDSCFWARLEEAKDLLEIIKRDGDVRKLAPIWKSLENFERYARGLIERKEVSKDVIAKNS 600
           QDSCFWAR+EEA++ L  ++ + D RK   +W+++  FERYA  L+ER EVSKDV+AKNS
Sbjct: 541 QDSCFWARVEEAREWLTSLRSENDPRKKELLWENINKFERYASNLVERMEVSKDVVAKNS 600

Query: 601 SYTLWAQELRALK 607
           SYTLW ++LR LK
Sbjct: 601 SYTLWVEDLRELK 608

BLAST of Csa4G496760 vs. NCBI nr
Match: gi|720045666|ref|XP_010270277.1| (PREDICTED: lipase-like PAD4 [Nelumbo nucifera])

HSP 1 Score: 644.4 bits (1661), Expect = 2.0e-181
Identity = 312/606 (51.49%), Postives = 417/606 (68.81%), Query Frame = 1

Query: 1   MESEASTFESCHVMAALLGSTPLLLQSWEFCAAANAASPESFTTVVIDDVAYVGFSGVQV 60
           ME E   FE+  ++A  L STP+L +SW  C+ ANA +P  F    I DV Y+ FSG+QV
Sbjct: 1   MEMETELFETSEMLAVFLASTPVLSESWRLCSHANATAPGGFIVNQIGDVGYIAFSGIQV 60

Query: 61  LPRCGGGGRELVALDGEGVEAELFWPLNRHREELQEPAMADSGILKMFVDIYTHKNLVET 120
           +      G  LV L+G       F P  R  ++ +EP M  SG L +F  IY +      
Sbjct: 61  ISGLNPNGNNLVLLNGTSSGLS-FSP--REVDDSEEPPMVHSGFLHIFHSIYNNPCFQNQ 120

Query: 121 ITKVMERSKSIVITGHSLGGAAATLCTLWLLSFLHTKTHHHPILCITFGSPLIGNESLSR 180
           I+ +M+ SKS+++TGHS+GGA A+L TLWLLS+L + +    +LCITFGSPL+GNESLS+
Sbjct: 121 ISMLMQNSKSLIVTGHSVGGATASLATLWLLSYLQSLSSSFSVLCITFGSPLLGNESLSK 180

Query: 181 AIQRERWCGKFCHVVSNHDIMPRLLSTPLSSLSPKLHILLRYWHLSMASPTFGKLATQLT 240
            I RERW GKFCHVV  +DI+PRLL  PL S++ +L+ +L++WHLSM +P +G L + L+
Sbjct: 181 TILRERWGGKFCHVVLKNDIVPRLLFAPLDSIATQLNHILQFWHLSM-TPQYGHLVSGLS 240

Query: 241 EREKEELFHIVLAHSNRISDLGEGTVQSQFWPFGNFFFCSEHGAICLDNAISVLKMLYLM 300
             EK E +H +L H+   +   +G+ +  +WPFG++ F S  GA+C++NA SV++MLYLM
Sbjct: 241 SEEKTEFYHYILNHTAAYAAQKDGSKRCSYWPFGSYLFFSGEGAVCIENATSVIQMLYLM 300

Query: 301 LKTSAPNLSIEDHLNYGYHVKKVGVQYMERKNFNSSCPPNSSYEAGLALALQSAGIPFQD 360
             T + N  I+DHL YG    KV    + R++F     P S YEAG++LA++++GI  QD
Sbjct: 301 FTTGSANSCIDDHLKYGDVAAKVSQLLLMRRSFTQGILPESGYEAGISLAIEASGIAPQD 360

Query: 361 EVAQIAEHCLRTASRIGQTPNMNAAKLAISLSKITPYRAEIEWYKASCEEADNQLGYYDC 420
            V   A  CL+ A ++G+ PN+N+A LAI L+K+TPYRA+IEWYK +C+ +++Q+GYYD 
Sbjct: 361 TVVNPARECLKMAKQMGRKPNLNSASLAIGLAKVTPYRAQIEWYKETCDNSEDQMGYYDS 420

Query: 421 FKKEDASVRHDRVNMNRHKLATFWNRVINMWENNELPPDFNTRAKWVNASQFYKLLVEPL 480
           FK   AS +  +VNMNR KLA FWN VI M E N+LP DF+ RAKWVNASQFYKLLVEPL
Sbjct: 421 FKLRGASKKDSQVNMNRFKLAVFWNNVIRMLETNQLPHDFHRRAKWVNASQFYKLLVEPL 480

Query: 481 DIAEYYHRDMHIVHGHYLKCGRERRYEIFDKWWRGREVTEEGNTQRMKYASLTQDSCFWA 540
           DIAEYY   MH   GHYL  GRERRYEIFD+WWRGRE   E N  R ++ASLTQDSCFWA
Sbjct: 481 DIAEYYRTGMHKTKGHYLTHGRERRYEIFDRWWRGREDVSEDNNSRTRFASLTQDSCFWA 540

Query: 541 RLEEAKDLLEIIKRDGDVRKLAPIWKSLENFERYARGLIERKEVSKDVIAKNSSYTLWAQ 600
           R+E AK+ ++  K + D  KLAP+W+S+  FE YA  L+E+KEVSKDV+AKNSSY+LW +
Sbjct: 541 RVEMAKEWMQNAKGETDPHKLAPLWESMNRFELYASQLVEKKEVSKDVVAKNSSYSLWLE 600

Query: 601 ELRALK 607
           E +  K
Sbjct: 601 EWKEFK 602

BLAST of Csa4G496760 vs. NCBI nr
Match: gi|743826816|ref|XP_011022873.1| (PREDICTED: lipase-like PAD4 [Populus euphratica])

HSP 1 Score: 633.6 bits (1633), Expect = 3.5e-178
Identity = 310/607 (51.07%), Postives = 420/607 (69.19%), Query Frame = 1

Query: 1   MESEASTFESCHVMAALLGSTPLLLQSWEFCAAANAASPESFTTVVIDDVAYVGFSGVQV 60
           M++E S FE+  ++A  L STPLL +SW  C  A A SP+SF    + ++ YV F G   
Sbjct: 1   MDTETSPFETSGMLANFLASTPLLTESWRLCNLAQAKSPQSFVAEQVGNIGYVAFPGTLF 60

Query: 61  LPRCGGGGRELVALDGEGVEA-ELFWPLNRHREELQEPAMADSGILKMFVDIYTHKNLVE 120
           +       + LV L    V   +LF PL+  + E +EP M    +L++F +IY+  +   
Sbjct: 61  VSGSDPSFKNLVRLPVHDVAGNDLFAPLH-DQNEGEEPVMVQGALLRIFENIYSDPSFQN 120

Query: 121 TITKVMERSKSIVITGHSLGGAAATLCTLWLLSFLHTKTHHHPILCITFGSPLIGNESLS 180
            ++ +M+ S+SI+ TGHS+GG  A+L  LWLLS+L + + +  +LCITFGSPL+GNE+LS
Sbjct: 121 QMSTLMQTSQSIIFTGHSIGGTTASLAALWLLSYLQSNSPNLSLLCITFGSPLLGNETLS 180

Query: 181 RAIQRERWCGKFCHVVSNHDIMPRLLSTPLSSLSPKLHILLRYWHLSMASPTFGKLATQL 240
           RAI RERW GKFCHVVS +D+MPR+L  PL  ++P +  LL++WHL M SP  G LA Q 
Sbjct: 181 RAILRERWGGKFCHVVSKYDLMPRILFAPLDPIAPLIKPLLQFWHLYMTSPHLGLLAVQR 240

Query: 241 TEREKEELFHIVLAHSNRISDLGEGTVQSQFWPFGNFFFCSEHGAICLDNAISVLKMLYL 300
            +  + E+F  VL H  R  + GE  V   F PFGN+FFCSE GAIC+DN  SV+KM+YL
Sbjct: 241 NDDYEAEIFKFVLVHLRRSVEAGEEAVTGVFRPFGNYFFCSEDGAICVDNVESVIKMMYL 300

Query: 301 MLKTSAPNLSIEDHLNYGYHVKKVGVQYMERKNFNSSCPPNSSYEAGLALALQSAGIPFQ 360
           +L T + + SIEDHL YG +V+++  Q++ER++F     P SSYEAG+ LALQS+GI  Q
Sbjct: 301 LLATGSSSCSIEDHLKYGDYVERISSQFLERRSFMEGELPESSYEAGVVLALQSSGISCQ 360

Query: 361 DEVAQIAEHCLRTASRIGQTPNMNAAKLAISLSKITPYRAEIEWYKASCEEADNQLGYYD 420
           + VA  A+ CL+ A R+G+TPN+N A LAI LS+I PYRAEIEWYK  C+ +D+Q+GYYD
Sbjct: 361 EPVAGPAKDCLKAARRMGRTPNLNCANLAIKLSRINPYRAEIEWYKELCDRSDDQMGYYD 420

Query: 421 CFKKEDASVRHDRVNMNRHKLATFWNRVINMWENNELPPDFNTRAKWVNASQFYKLLVEP 480
            FK+  AS R  +VN+NRHKLA FW+ VI++ E+N+LP DF+   KWV +SQFYKLLVEP
Sbjct: 421 SFKRRGASKRDFKVNLNRHKLAKFWDNVIDLMESNQLPHDFHRHGKWVYSSQFYKLLVEP 480

Query: 481 LDIAEYYHRDMHIVHGHYLKCGRERRYEIFDKWWRGREVTEEGNTQRMKYASLTQDSCFW 540
           LDIAEYY   MH   GHY+  GRERRY+IFD+WW+GR V +E N +R KYASLTQD+CFW
Sbjct: 481 LDIAEYYRTGMHHSKGHYINHGRERRYQIFDRWWKGRNVRDEEN-KRSKYASLTQDTCFW 540

Query: 541 ARLEEAKDLLEIIKRDGDVRKLAPIWKSLENFERYARGLIERKEVSKDVIAKNSSYTLWA 600
           A++EEA+ LL+ +    D    A +W+ +++F  YA+ L+E KEVS DV+ KNSSY+LW 
Sbjct: 541 AKVEEARGLLDDVGNTRDPSHSALLWEKMDSFASYAKTLVETKEVSADVVLKNSSYSLWL 600

Query: 601 QELRALK 607
           ++   LK
Sbjct: 601 KDYNELK 605