Csa4G154320 (gene) Cucumber (Chinese Long) v2

BLAST of Csa4G154320 vs. Swiss-Prot
Match: PGIP_PYRCO (Polygalacturonase inhibitor OS=Pyrus communis GN=PGIP PE=1 SV=1)

HSP 1 Score: 428.3 bits (1100), Expect = 7.5e-119
Identity = 212/323 (65.63%), Postives = 239/323 (73.99%), Query Frame = 1

Query: 6   LLLLLLLLFFFTVSYAELCHPNDKKVLLNIKKAFNNPYILTSWKPEEDCCTWYCVECDRK 65
           L L LL       + ++LC+P+DKKVLL IKKAF +PY+L SWK + DCC WYCV CD  
Sbjct: 9   LSLTLLFSSVLNPALSDLCNPDDKKVLLQIKKAFGDPYVLASWKSDTDCCDWYCVTCDST 68

Query: 66  SHRIIALTVFADDKLSGPIPPFVGDLPFLENLMFHKLPNLIGPIPPTIAKLNNLKYLDLS 125
           ++RI +LT+FA  ++SG IP  VGDLP+LE L FHK PNL GPI P IAKL  LK L LS
Sbjct: 69  TNRINSLTIFAG-QVSGQIPALVGDLPYLETLEFHKQPNLTGPIQPAIAKLKGLKSLRLS 128

Query: 126 WNGLSGPVPSFLGSLSNLDVLDLSFNRFTGSIPSSLANLRRLGTLHLDRNKLTGPIPESF 185
           W  LSG VP FL  L NL  LDLSFN  TG+IPSSL+ L  LG L LDRNKLTG IP SF
Sbjct: 129 WTNLSGSVPDFLSQLKNLTFLDLSFNNLTGAIPSSLSELPNLGALRLDRNKLTGHIPISF 188

Query: 186 GNFKGKVPYLYLSHNQLSGKIPISMGKVDFNYIDLSRNKLVGDGSLIFGSKKTTEIVDLS 245
           G F G VP LYLSHNQLSG IP S  ++DF  IDLSRNKL GD S+IFG  KTT+IVDLS
Sbjct: 189 GQFIGNVPDLYLSHNQLSGNIPTSFAQMDFTSIDLSRNKLEGDASVIFGLNKTTQIVDLS 248

Query: 246 RNLLEFNMSKVVFPRTLTYLDLNHNKIFGEIPTEVVKLELQMFNVSYNALCGRIPMGGKL 305
           RNLLEFN+SKV FP +LT LD+NHNKI+G IP E  +L  Q  NVSYN LCG+IP+GGKL
Sbjct: 249 RNLLEFNLSKVEFPTSLTSLDINHNKIYGSIPVEFTQLNFQFLNVSYNRLCGQIPVGGKL 308

Query: 306 QSFDVYSYFHNKCLCGKPLGSCK 329
           QSFD YSYFHN+CLCG PL SCK
Sbjct: 309 QSFDEYSYFHNRCLCGAPLPSCK 330

BLAST of Csa4G154320 vs. Swiss-Prot
Match: PGIP_VITVI (Polygalacturonase inhibitor OS=Vitis vinifera GN=pgip PE=1 SV=1)

HSP 1 Score: 409.5 bits (1051), Expect = 3.6e-113
Identity = 203/322 (63.04%), Postives = 236/322 (73.29%), Query Frame = 1

Query: 7   LLLLLLLFFFTVSYAELCHPNDKKVLLNIKKAFNNPYILTSWKPEEDCCTWYCVECDRKS 66
           LLL+LL      S +E C+P DKKVLL IKKA +NPYIL SW P  DCC WYCVECD  +
Sbjct: 13  LLLVLLATRPCPSLSERCNPKDKKVLLQIKKALDNPYILASWNPNTDCCGWYCVECDLTT 72

Query: 67  HRIIALTVFADDKLSGPIPPFVGDLPFLENLMFHKLPNLIGPIPPTIAKLNNLKYLDLSW 126
           HRI +LT+F+  +LSG IP  VGDLPFLE L+F KL NL G IPP IAKL +LK + LSW
Sbjct: 73  HRINSLTIFSG-QLSGQIPDAVGDLPFLETLIFRKLSNLTGQIPPAIAKLKHLKMVRLSW 132

Query: 127 NGLSGPVPSFLGSLSNLDVLDLSFNRFTGSIPSSLANLRRLGTLHLDRNKLTGPIPESFG 186
             LSGPVP+F   L NL  LDLSFN  +G IP SL+ L  LG LHLDRN LTGPIP+SFG
Sbjct: 133 TNLSGPVPAFFSELKNLTYLDLSFNNLSGPIPGSLSLLPNLGALHLDRNHLTGPIPDSFG 192

Query: 187 NFKGKVPYLYLSHNQLSGKIPISMGKVDFNYIDLSRNKLVGDGSLIFGSKKTTEIVDLSR 246
            F G  P L+LSHNQLSGKIP S    D N +DLSRNKL GD S+ F + K+T+IVD SR
Sbjct: 193 KFAGSTPGLHLSHNQLSGKIPYSFRGFDPNVMDLSRNKLEGDLSIFFNANKSTQIVDFSR 252

Query: 247 NLLEFNMSKVVFPRTLTYLDLNHNKIFGEIPTEVVKLELQMFNVSYNALCGRIPMGGKLQ 306
           NL +F++S+V FP++LT LDL+HNKI G +P  +  L+LQ  NVSYN LCG+IP+GGKLQ
Sbjct: 253 NLFQFDLSRVEFPKSLTSLDLSHNKIAGSLPEMMTSLDLQFLNVSYNRLCGKIPVGGKLQ 312

Query: 307 SFDVYSYFHNKCLCGKPLGSCK 329
           SFD  SYFHN+CLCG PL SCK
Sbjct: 313 SFDYDSYFHNRCLCGAPLQSCK 333

BLAST of Csa4G154320 vs. Swiss-Prot
Match: PGIP1_ARATH (Polygalacturonase inhibitor 1 OS=Arabidopsis thaliana GN=PGIP1 PE=2 SV=1)

HSP 1 Score: 400.6 bits (1028), Expect = 1.7e-110
Identity = 198/325 (60.92%), Postives = 234/325 (72.00%), Query Frame = 1

Query: 6   LLLLLLLLFFFTVSYAELCHPNDKKVLLNIKKAFNNPYILTSWKPEEDCCTWYCVECDRK 65
           L LL L  F  T    +LC+ NDK  LL IKK+ NNPY L SW P+ DCC+WYC+EC   
Sbjct: 7   LCLLFLFTFLTTCLSKDLCNQNDKNTLLKIKKSLNNPYHLASWDPQTDCCSWYCLECGDA 66

Query: 66  S--HRIIALTVFADDKLSGPIPPFVGDLPFLENLMFHKLPNLIGPIPPTIAKLNNLKYLD 125
           +  HR+ ALT+F+  ++SG IP  VGDLP+LE L+F KL NL G I PTIAKL NL+ L 
Sbjct: 67  TVNHRVTALTIFSG-QISGQIPAEVGDLPYLETLVFRKLSNLTGTIQPTIAKLKNLRMLR 126

Query: 126 LSWNGLSGPVPSFLGSLSNLDVLDLSFNRFTGSIPSSLANLRRLGTLHLDRNKLTGPIPE 185
           LSW  L+GP+P F+  L NL+ L+LSFN  +GSIPSSL+ L ++  L L RNKLTG IPE
Sbjct: 127 LSWTNLTGPIPDFISQLKNLEFLELSFNDLSGSIPSSLSTLPKILALELSRNKLTGSIPE 186

Query: 186 SFGNFKGKVPYLYLSHNQLSGKIPISMGKVDFNYIDLSRNKLVGDGSLIFGSKKTTEIVD 245
           SFG+F G VP L LSHNQLSG IP S+G +DFN IDLSRNKL GD S++FGS KTT  +D
Sbjct: 187 SFGSFPGTVPDLRLSHNQLSGPIPKSLGNIDFNRIDLSRNKLQGDASMLFGSNKTTWSID 246

Query: 246 LSRNLLEFNMSKVVFPRTLTYLDLNHNKIFGEIPTEVVKLELQMFNVSYNALCGRIPMGG 305
           LSRN+ +F++SKV  P+TL  LDLNHN I G IP +  +  LQ FNVSYN LCG IP GG
Sbjct: 247 LSRNMFQFDISKVDIPKTLGILDLNHNGITGNIPVQWTEAPLQFFNVSYNKLCGHIPTGG 306

Query: 306 KLQSFDVYSYFHNKCLCGKPLGSCK 329
           KLQ+FD YSYFHNKCLCG PL  CK
Sbjct: 307 KLQTFDSYSYFHNKCLCGAPLEICK 330

BLAST of Csa4G154320 vs. Swiss-Prot
Match: PGIP2_ARATH (Polygalacturonase inhibitor 2 OS=Arabidopsis thaliana GN=PGIP2 PE=2 SV=2)

HSP 1 Score: 398.7 bits (1023), Expect = 6.3e-110
Identity = 199/325 (61.23%), Postives = 235/325 (72.31%), Query Frame = 1

Query: 7   LLLLLLLFFFTVSYA-ELCHPNDKKVLLNIKKAFNNPYILTSWKPEEDCCTWYCVECDRK 66
           L LLL     T S A +LCH +DK  LL IKK+ NNPY L SW P+ DCC+WYC+EC   
Sbjct: 7   LFLLLSTLLLTTSLAKDLCHKDDKTTLLKIKKSLNNPYHLASWDPKTDCCSWYCLECGDA 66

Query: 67  S--HRIIALTVFADDKLSGPIPPFVGDLPFLENLMFHKLPNLIGPIPPTIAKLNNLKYLD 126
           +  HR+ +L +  D ++SG IPP VGDLP+L +L+F KL NL G I PTIAKL NL +L 
Sbjct: 67  TVNHRVTSL-IIQDGEISGQIPPEVGDLPYLTSLIFRKLTNLTGHIQPTIAKLKNLTFLR 126

Query: 127 LSWNGLSGPVPSFLGSLSNLDVLDLSFNRFTGSIPSSLANLRRLGTLHLDRNKLTGPIPE 186
           LSW  L+GPVP FL  L NL+ +DLSFN  +GSIPSSL++LR+L  L L RNKLTGPIPE
Sbjct: 127 LSWTNLTGPVPEFLSQLKNLEYIDLSFNDLSGSIPSSLSSLRKLEYLELSRNKLTGPIPE 186

Query: 187 SFGNFKGKVPYLYLSHNQLSGKIPISMGKVDFNYIDLSRNKLVGDGSLIFGSKKTTEIVD 246
           SFG F GKVP L+LSHNQLSG IP S+G  DF  IDLSRNKL GD S++FG+KKTT IVD
Sbjct: 187 SFGTFSGKVPSLFLSHNQLSGTIPKSLGNPDFYRIDLSRNKLQGDASILFGAKKTTWIVD 246

Query: 247 LSRNLLEFNMSKVVFPRTLTYLDLNHNKIFGEIPTEVVKLELQMFNVSYNALCGRIPMGG 306
           +SRN+ +F++SKV   +TL  LD+NHN I G IP E  K   Q+ NVSYN LCGRIP G 
Sbjct: 247 ISRNMFQFDLSKVKLAKTLNNLDMNHNGITGSIPAEWSKAYFQLLNVSYNRLCGRIPKGE 306

Query: 307 KLQSFDVYSYFHNKCLCGKPLGSCK 329
            +Q FD YS+FHNKCLCG PL SCK
Sbjct: 307 YIQRFDSYSFFHNKCLCGAPLPSCK 330

BLAST of Csa4G154320 vs. Swiss-Prot
Match: PGIP1_PHAVU (Polygalacturonase inhibitor 1 OS=Phaseolus vulgaris GN=PGIP1 PE=1 SV=1)

HSP 1 Score: 275.4 bits (703), Expect = 8.1e-73
Identity = 141/331 (42.60%), Postives = 207/331 (62.54%), Query Frame = 1

Query: 3   SPNLLLLLLLLFFFTVSYAELCHPNDKKVLLNIKKAFNNPYILTSWKPEEDCC--TWYCV 62
           S +L ++L++L     + +ELC+P DK+ LL IKK   NP  L+SW P  DCC  TW  V
Sbjct: 11  SSSLSIILVILVSLRTALSELCNPQDKQALLQIKKDLGNPTTLSSWLPTTDCCNRTWLGV 70

Query: 63  ECDR--KSHRIIALTVFADDKLSG-PIPPFVGDLPFLENLMFHKLPNLIGPIPPTIAKLN 122
            CD   +++R+  L +   +     PIP  + +LP+L  L    + NL+GPIPP IAKL 
Sbjct: 71  LCDTDTQTYRVNNLDLSGHNLPKPYPIPSSLANLPYLNFLYIGGINNLVGPIPPAIAKLT 130

Query: 123 NLKYLDLSWNGLSGPVPSFLGSLSNLDVLDLSFNRFTGSIPSSLANLRRLGTLHLDRNKL 182
            L YL ++   +SG +P FL  +  L  LD S+N  +G++P S+++L  LG +  D N++
Sbjct: 131 QLHYLYITHTNVSGAIPDFLSQIKTLVTLDFSYNALSGTLPPSISSLPNLGGITFDGNRI 190

Query: 183 TGPIPESFGNFKGKVPYLYLSHNQLSGKIPISMGKVDFNYIDLSRNKLVGDGSLIFGSKK 242
           +G IP+S+G+F      + +S N+L+GKIP +   ++  ++DLSRN L GD S++FGS K
Sbjct: 191 SGAIPDSYGSFSKLFTAMTISRNRLTGKIPPTFANLNLAFVDLSRNMLEGDASVLFGSDK 250

Query: 243 TTEIVDLSRNLLEFNMSKVVFPRTLTYLDLNHNKIFGEIPTEVVKLE-LQMFNVSYNALC 302
            T+ + L++N L F++ KV   + L  LDL +N+I+G +P  + +L+ LQ  NVS+N LC
Sbjct: 251 NTKKIHLAKNSLAFDLGKVGLSKNLNGLDLRNNRIYGTLPQGLTQLKFLQSLNVSFNNLC 310

Query: 303 GRIPMGGKLQSFDVYSYFHNKCLCGKPLGSC 328
           G IP GG L+ FDV SY +NKCLCG PL SC
Sbjct: 311 GEIPQGGNLKRFDVSSYANNKCLCGSPLPSC 341

BLAST of Csa4G154320 vs. TrEMBL
Match: K7QLL6_CUCSA (Polygalacturonase-inhibiting protein 2 OS=Cucumis sativus GN=PGIP2 PE=2 SV=1)

HSP 1 Score: 459.1 bits (1180), Expect = 4.4e-126
Identity = 218/320 (68.12%), Postives = 259/320 (80.94%), Query Frame = 1

Query: 10  LLLLFFFTVSYAELCHPNDKKVLLNIKKAFNNPYILTSWKPEEDCCTWYCVECDRKSHRI 69
           L+  FF T+S AELC+PNDKKVLL IKKAFNNPYILTSW P+ DCC WYCV+C+R +HRI
Sbjct: 16  LIFFFFVTISSAELCNPNDKKVLLKIKKAFNNPYILTSWDPQTDCCHWYCVKCNRTTHRI 75

Query: 70  IALTVFADDKLSGPIPPFVGDLPFLENLMFHKLPNLIGPIPPTIAKLNNLKYLDLSWNGL 129
           I+LT+FADD+L+G IPP VGDLPFL+ LM HKLPNL GPI PTIAKL NL +LDLSWNGL
Sbjct: 76  ISLTIFADDRLTGQIPPEVGDLPFLQTLMLHKLPNLTGPIQPTIAKLRNLVFLDLSWNGL 135

Query: 130 SGPVPSFLGSLSNLDVLDLSFNRFTGSIPSSLANLRRLGTLHLDRNKLTGPIPESFGNFK 189
           SG +P  L +L NL +L LSFN+ TG IPSSL+ L  LG L LDRN+LTG IPESFG F 
Sbjct: 136 SGEIPDSLSTLKNLFILTLSFNKLTGEIPSSLSELPNLGGLRLDRNQLTGQIPESFGYFS 195

Query: 190 G-KVPYLYLSHNQLSGKIPISMGKVDFNYIDLSRNKLVGDGSLIFGSKKTTEIVDLSRNL 249
           G + P L LSHNQLSGKIP S+ +++   IDLSRNKL GD S+ FG+ K  +I+DLSRNL
Sbjct: 196 GEQAPDLILSHNQLSGKIPSSLSRLNSTSIDLSRNKLEGDASMFFGANKMIQILDLSRNL 255

Query: 250 LEFNMSKVVFPRTLTYLDLNHNKIFGEIPTEVVKLELQMFNVSYNALCGRIPMGGKLQSF 309
           LEFN+S+VVFP++LT LDLNHNK++G +P  + +L+LQ  NVSYN LCG IP+GG +QSF
Sbjct: 256 LEFNLSEVVFPQSLTSLDLNHNKVYGGLPPVMTELKLQYLNVSYNRLCGPIPVGGDMQSF 315

Query: 310 DVYSYFHNKCLCGKPLGSCK 329
           D+YSYFHNKCLCG PLG CK
Sbjct: 316 DLYSYFHNKCLCGAPLGDCK 335

BLAST of Csa4G154320 vs. TrEMBL
Match: A0A067DC51_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g020203mg PE=4 SV=1)

HSP 1 Score: 438.7 bits (1127), Expect = 6.1e-120
Identity = 213/325 (65.54%), Postives = 250/325 (76.92%), Query Frame = 1

Query: 10  LLLLFFF-----TVSYAELCHPNDKKVLLNIKKAFNNPYILTSWKPEEDCCTWYCVECDR 69
           LL LFFF     + S ++LC+PNDKKVLL  KK+ NNPY+L SW P+ DCC WYCV CD 
Sbjct: 6   LLSLFFFLCLCISPSLSDLCNPNDKKVLLKFKKSLNNPYVLASWNPKTDCCDWYCVTCDL 65

Query: 70  KSHRIIALTVFADDKLSGPIPPFVGDLPFLENLMFHKLPNLIGPIPPTIAKLNNLKYLDL 129
            ++RI +LT+FA D L G IPP VGDLP+LE LMFHKLP+L GPI P IAKL NLK L +
Sbjct: 66  TTNRINSLTIFAGD-LPGQIPPEVGDLPYLETLMFHKLPSLTGPIQPAIAKLKNLKTLRI 125

Query: 130 SWNGLSGPVPSFLGSLSNLDVLDLSFNRFTGSIPSSLANLRRLGTLHLDRNKLTGPIPES 189
           SW  +SGPVP F+  L+NL  L+LSFN  +G+IP SL+ L++LG LHLDRNKLTG IPES
Sbjct: 126 SWTNISGPVPDFISQLTNLTFLELSFNNLSGTIPGSLSKLQKLGALHLDRNKLTGSIPES 185

Query: 190 FGNFKGKVPYLYLSHNQLSGKIPISMGKVDFNYIDLSRNKLVGDGSLIFGSKKTTEIVDL 249
           FG F G +P LYLSHNQLSGKIP S+G +DFN IDLSRNKL GD S +FG  KTT+ +D+
Sbjct: 186 FGTFTGSIPDLYLSHNQLSGKIPASLGSMDFNTIDLSRNKLEGDASFLFGLNKTTQRIDV 245

Query: 250 SRNLLEFNMSKVVFPRTLTYLDLNHNKIFGEIPTEVVKLE-LQMFNVSYNALCGRIPMGG 309
           SRNLLEFN+SKV FP++LT LDLNHNKIFG IP ++  LE L   NVSYN LCG IP+GG
Sbjct: 246 SRNLLEFNLSKVEFPQSLTNLDLNHNKIFGSIPAQITSLENLGFLNVSYNRLCGPIPVGG 305

Query: 310 KLQSFDVYSYFHNKCLCGKPLGSCK 329
           KLQSF    YFHN+CLCG PL SCK
Sbjct: 306 KLQSFGYTEYFHNRCLCGAPLESCK 329

BLAST of Csa4G154320 vs. TrEMBL
Match: Q8W3U5_9ROSI (Polygalacturonase-inhibitor protein OS=Citrus hybrid cultivar GN=PGIP1 PE=4 SV=1)

HSP 1 Score: 438.3 bits (1126), Expect = 8.0e-120
Identity = 212/325 (65.23%), Postives = 250/325 (76.92%), Query Frame = 1

Query: 10  LLLLFFF-----TVSYAELCHPNDKKVLLNIKKAFNNPYILTSWKPEEDCCTWYCVECDR 69
           LL LFFF     + S ++LC+PNDKKVLL  KK+ NNPY+L SW P+ DCC WYCV CD 
Sbjct: 6   LLSLFFFLCLCISPSLSDLCNPNDKKVLLKFKKSLNNPYVLASWNPKTDCCDWYCVTCDL 65

Query: 70  KSHRIIALTVFADDKLSGPIPPFVGDLPFLENLMFHKLPNLIGPIPPTIAKLNNLKYLDL 129
            ++RI +LT+FA D L G IPP VGDLP+LE LMFHKLP+L GP+ P IAKL NLK L +
Sbjct: 66  TTNRINSLTIFAGD-LPGQIPPEVGDLPYLETLMFHKLPSLTGPVQPAIAKLKNLKTLRI 125

Query: 130 SWNGLSGPVPSFLGSLSNLDVLDLSFNRFTGSIPSSLANLRRLGTLHLDRNKLTGPIPES 189
           SW  +SGPVP F+  L+NL  L+LSFN  +G+IP SL+ L++LG LHLDRNKLTG IPES
Sbjct: 126 SWTNISGPVPDFISQLTNLTFLELSFNNLSGTIPGSLSKLQKLGALHLDRNKLTGSIPES 185

Query: 190 FGNFKGKVPYLYLSHNQLSGKIPISMGKVDFNYIDLSRNKLVGDGSLIFGSKKTTEIVDL 249
           FG F G +P LYLSHNQLSGKIP S+G +DFN IDLSRNKL GD S +FG  KTT+ +D+
Sbjct: 186 FGTFTGSIPDLYLSHNQLSGKIPASLGSMDFNTIDLSRNKLEGDASFLFGLNKTTQRIDV 245

Query: 250 SRNLLEFNMSKVVFPRTLTYLDLNHNKIFGEIPTEVVKLE-LQMFNVSYNALCGRIPMGG 309
           SRNLLEFN+SKV FP++LT LDLNHNKIFG IP ++  LE L   NVSYN LCG IP+GG
Sbjct: 246 SRNLLEFNLSKVEFPQSLTNLDLNHNKIFGSIPAQITSLENLGFLNVSYNRLCGPIPVGG 305

Query: 310 KLQSFDVYSYFHNKCLCGKPLGSCK 329
           KLQSF    YFHN+CLCG PL SCK
Sbjct: 306 KLQSFGYTEYFHNRCLCGAPLESCK 329

BLAST of Csa4G154320 vs. TrEMBL
Match: Q9ZWH6_PONTR (Polygalacturonase inhibitor OS=Poncirus trifoliata GN=PGIP PE=4 SV=1)

HSP 1 Score: 437.2 bits (1123), Expect = 1.8e-119
Identity = 212/326 (65.03%), Postives = 251/326 (76.99%), Query Frame = 1

Query: 1   MHSPNLLLLLLLLFFFT-VSYAELCHPNDKKVLLNIKKAFNNPYILTSWKPEEDCCTWYC 60
           M + +LL L   L  FT +S ++LC+PNDK+VLLN KKA NNPY+L SW P+ DCC WYC
Sbjct: 1   MSNTSLLSLFFFLSLFTSLSLSDLCNPNDKRVLLNFKKALNNPYVLASWNPKTDCCDWYC 60

Query: 61  VECDRKSHRIIALTVFADDKLSGPIPPFVGDLPFLENLMFHKLPNLIGPIPPTIAKLNNL 120
           V CD  ++RI +LT+FA D L G IPP VGDLP+LE LMFHKLP+L GPI P IAKL NL
Sbjct: 61  VTCDLTTNRINSLTIFAGD-LPGQIPPEVGDLPYLETLMFHKLPSLTGPIQPAIAKLKNL 120

Query: 121 KYLDLSWNGLSGPVPSFLGSLSNLDVLDLSFNRFTGSIPSSLANLRRLGTLHLDRNKLTG 180
           K L +SW  +SGPVP F+  L+NL  L+LSFN  +G+IPSSL+ LR+LG LHLDRNKLTG
Sbjct: 121 KMLRISWTNISGPVPDFISQLTNLTFLELSFNNLSGTIPSSLSKLRKLGALHLDRNKLTG 180

Query: 181 PIPESFGNFKGKVPYLYLSHNQLSGKIPISMGKVDFNYIDLSRNKLVGDGSLIFGSKKTT 240
            IP+SFG F G +P LYLSHNQLSGKIP S+G +DFN IDLSR+KL GD S +FG  KTT
Sbjct: 181 SIPDSFGTFTGSIPDLYLSHNQLSGKIPASLGSMDFNTIDLSRSKLEGDASFLFGLNKTT 240

Query: 241 EIVDLSRNLLEFNMSKVVFPRTLTYLDLNHNKIFGEIPTEVVKLE-LQMFNVSYNALCGR 300
           + +D+SRNLLEFN+SKV FP +LT LDLNHNKIFG IP ++  LE L   NVSYN LCG 
Sbjct: 241 QRIDVSRNLLEFNLSKVEFPESLTNLDLNHNKIFGSIPAQITSLENLGFLNVSYNRLCGP 300

Query: 301 IPMGGKLQSFDVYSYFHNKCLCGKPL 325
           IP+GGKLQSF    YFHN+CLCG PL
Sbjct: 301 IPVGGKLQSFGYKEYFHNRCLCGAPL 325

BLAST of Csa4G154320 vs. TrEMBL
Match: Q8W3U6_9ROSI (Polygalacturonase-inhibitor protein OS=Citrus hybrid cultivar GN=PGIP3 PE=4 SV=1)

HSP 1 Score: 437.2 bits (1123), Expect = 1.8e-119
Identity = 212/325 (65.23%), Postives = 250/325 (76.92%), Query Frame = 1

Query: 10  LLLLFFF-----TVSYAELCHPNDKKVLLNIKKAFNNPYILTSWKPEEDCCTWYCVECDR 69
           LL LFFF     + S ++LC+PNDKKVLL  KK+ NNPY+L SW P+ DCC WYCV CD 
Sbjct: 6   LLSLFFFLCLCISPSLSDLCNPNDKKVLLKFKKSLNNPYVLASWNPKTDCCDWYCVTCDL 65

Query: 70  KSHRIIALTVFADDKLSGPIPPFVGDLPFLENLMFHKLPNLIGPIPPTIAKLNNLKYLDL 129
            ++RI +LT+FA D L G IPP VGDLP+LE LMFHKLP+L GPI P IAKL NLK L +
Sbjct: 66  TTNRINSLTIFAGD-LPGQIPPEVGDLPYLETLMFHKLPSLTGPIQPAIAKLKNLKTLRI 125

Query: 130 SWNGLSGPVPSFLGSLSNLDVLDLSFNRFTGSIPSSLANLRRLGTLHLDRNKLTGPIPES 189
           SW  +SGPVP F+  L+NL  L+LSFN  +G+IP SL+ L++LG LHLDRNKLTG IPES
Sbjct: 126 SWTNISGPVPDFISQLTNLTFLELSFNNLSGTIPGSLSKLQKLGALHLDRNKLTGSIPES 185

Query: 190 FGNFKGKVPYLYLSHNQLSGKIPISMGKVDFNYIDLSRNKLVGDGSLIFGSKKTTEIVDL 249
           FG F G +P LYLSHNQLSGKIP S+G +DFN IDLSRNKL GD S +FG  KTT+ +D+
Sbjct: 186 FGTFTGSIPDLYLSHNQLSGKIPASLGSMDFNTIDLSRNKLEGDASFLFGLNKTTQRIDV 245

Query: 250 SRNLLEFNMSKVVFPRTLTYLDLNHNKIFGEIPTEVVKLE-LQMFNVSYNALCGRIPMGG 309
           SRNLLEFN+SKV FP++LT LDLNHN+IFG IP ++  LE L   NVSYN LCG IP+GG
Sbjct: 246 SRNLLEFNLSKVEFPQSLTNLDLNHNEIFGSIPAQITSLENLGFLNVSYNRLCGPIPVGG 305

Query: 310 KLQSFDVYSYFHNKCLCGKPLGSCK 329
           KLQSF    YFHN+CLCG PL SCK
Sbjct: 306 KLQSFGYTEYFHNRCLCGAPLESCK 329

BLAST of Csa4G154320 vs. TAIR10
Match: AT5G06860.1 (AT5G06860.1 polygalacturonase inhibiting protein 1)

HSP 1 Score: 400.6 bits (1028), Expect = 9.4e-112
Identity = 198/325 (60.92%), Postives = 234/325 (72.00%), Query Frame = 1

Query: 6   LLLLLLLLFFFTVSYAELCHPNDKKVLLNIKKAFNNPYILTSWKPEEDCCTWYCVECDRK 65
           L LL L  F  T    +LC+ NDK  LL IKK+ NNPY L SW P+ DCC+WYC+EC   
Sbjct: 7   LCLLFLFTFLTTCLSKDLCNQNDKNTLLKIKKSLNNPYHLASWDPQTDCCSWYCLECGDA 66

Query: 66  S--HRIIALTVFADDKLSGPIPPFVGDLPFLENLMFHKLPNLIGPIPPTIAKLNNLKYLD 125
           +  HR+ ALT+F+  ++SG IP  VGDLP+LE L+F KL NL G I PTIAKL NL+ L 
Sbjct: 67  TVNHRVTALTIFSG-QISGQIPAEVGDLPYLETLVFRKLSNLTGTIQPTIAKLKNLRMLR 126

Query: 126 LSWNGLSGPVPSFLGSLSNLDVLDLSFNRFTGSIPSSLANLRRLGTLHLDRNKLTGPIPE 185
           LSW  L+GP+P F+  L NL+ L+LSFN  +GSIPSSL+ L ++  L L RNKLTG IPE
Sbjct: 127 LSWTNLTGPIPDFISQLKNLEFLELSFNDLSGSIPSSLSTLPKILALELSRNKLTGSIPE 186

Query: 186 SFGNFKGKVPYLYLSHNQLSGKIPISMGKVDFNYIDLSRNKLVGDGSLIFGSKKTTEIVD 245
           SFG+F G VP L LSHNQLSG IP S+G +DFN IDLSRNKL GD S++FGS KTT  +D
Sbjct: 187 SFGSFPGTVPDLRLSHNQLSGPIPKSLGNIDFNRIDLSRNKLQGDASMLFGSNKTTWSID 246

Query: 246 LSRNLLEFNMSKVVFPRTLTYLDLNHNKIFGEIPTEVVKLELQMFNVSYNALCGRIPMGG 305
           LSRN+ +F++SKV  P+TL  LDLNHN I G IP +  +  LQ FNVSYN LCG IP GG
Sbjct: 247 LSRNMFQFDISKVDIPKTLGILDLNHNGITGNIPVQWTEAPLQFFNVSYNKLCGHIPTGG 306

Query: 306 KLQSFDVYSYFHNKCLCGKPLGSCK 329
           KLQ+FD YSYFHNKCLCG PL  CK
Sbjct: 307 KLQTFDSYSYFHNKCLCGAPLEICK 330

BLAST of Csa4G154320 vs. TAIR10
Match: AT5G06870.1 (AT5G06870.1 polygalacturonase inhibiting protein 2)

HSP 1 Score: 398.7 bits (1023), Expect = 3.6e-111
Identity = 199/325 (61.23%), Postives = 235/325 (72.31%), Query Frame = 1

Query: 7   LLLLLLLFFFTVSYA-ELCHPNDKKVLLNIKKAFNNPYILTSWKPEEDCCTWYCVECDRK 66
           L LLL     T S A +LCH +DK  LL IKK+ NNPY L SW P+ DCC+WYC+EC   
Sbjct: 7   LFLLLSTLLLTTSLAKDLCHKDDKTTLLKIKKSLNNPYHLASWDPKTDCCSWYCLECGDA 66

Query: 67  S--HRIIALTVFADDKLSGPIPPFVGDLPFLENLMFHKLPNLIGPIPPTIAKLNNLKYLD 126
           +  HR+ +L +  D ++SG IPP VGDLP+L +L+F KL NL G I PTIAKL NL +L 
Sbjct: 67  TVNHRVTSL-IIQDGEISGQIPPEVGDLPYLTSLIFRKLTNLTGHIQPTIAKLKNLTFLR 126

Query: 127 LSWNGLSGPVPSFLGSLSNLDVLDLSFNRFTGSIPSSLANLRRLGTLHLDRNKLTGPIPE 186
           LSW  L+GPVP FL  L NL+ +DLSFN  +GSIPSSL++LR+L  L L RNKLTGPIPE
Sbjct: 127 LSWTNLTGPVPEFLSQLKNLEYIDLSFNDLSGSIPSSLSSLRKLEYLELSRNKLTGPIPE 186

Query: 187 SFGNFKGKVPYLYLSHNQLSGKIPISMGKVDFNYIDLSRNKLVGDGSLIFGSKKTTEIVD 246
           SFG F GKVP L+LSHNQLSG IP S+G  DF  IDLSRNKL GD S++FG+KKTT IVD
Sbjct: 187 SFGTFSGKVPSLFLSHNQLSGTIPKSLGNPDFYRIDLSRNKLQGDASILFGAKKTTWIVD 246

Query: 247 LSRNLLEFNMSKVVFPRTLTYLDLNHNKIFGEIPTEVVKLELQMFNVSYNALCGRIPMGG 306
           +SRN+ +F++SKV   +TL  LD+NHN I G IP E  K   Q+ NVSYN LCGRIP G 
Sbjct: 247 ISRNMFQFDLSKVKLAKTLNNLDMNHNGITGSIPAEWSKAYFQLLNVSYNRLCGRIPKGE 306

Query: 307 KLQSFDVYSYFHNKCLCGKPLGSCK 329
            +Q FD YS+FHNKCLCG PL SCK
Sbjct: 307 YIQRFDSYSFFHNKCLCGAPLPSCK 330

BLAST of Csa4G154320 vs. TAIR10
Match: AT3G12145.1 (AT3G12145.1 Leucine-rich repeat (LRR) family protein)

HSP 1 Score: 306.2 bits (783), Expect = 2.4e-83
Identity = 161/322 (50.00%), Postives = 206/322 (63.98%), Query Frame = 1

Query: 7   LLLLLLLFFFTVSYAELCHPNDKKVLLNIKKAFNNPYILTSWKPEEDCCT-WYCVECDRK 66
           L +   + F T+  +  C  NDK  LL IKKA  NP +L+SW P  DCCT W  VEC  +
Sbjct: 7   LSIFFSILFITLPSSYSCTENDKNALLQIKKALGNPPLLSSWNPRTDCCTGWTGVECTNR 66

Query: 67  SHRIIALTVFADDKLSGPIPPFVGDLPFLENLMFHKLPNLIGPIPPTIAKLNNLKYLDLS 126
             R+  L+V + + +SG I   +GDL  L  L F  LP+L G IP TI KL NL  L L 
Sbjct: 67  --RVTGLSVTSGE-VSGQISYQIGDLVDLRTLDFSYLPHLTGNIPRTITKLKNLNTLYLK 126

Query: 127 WNGLSGPVPSFLGSLSNLDVLDLSFNRFTGSIPSSLANLRRLGTLHLDRNKLTGPIPESF 186
              LSGP+P ++  L +L  LDLSFN+FTG IP SL+ + +L  + ++ NKLTG IP SF
Sbjct: 127 HTSLSGPIPDYISELKSLTFLDLSFNQFTGPIPGSLSQMPKLEAIQINDNKLTGSIPNSF 186

Query: 187 GNFKGKVPYLYLSHNQLSGKIPISMGKVDFNYIDLSRNKLVGDGSLIFGSKKTTEIVDLS 246
           G+F G VP LYLS+N+LSGKIP S+ K DFN +DLS N   GD  + FG  KTT  VDLS
Sbjct: 187 GSFVGNVPNLYLSNNKLSGKIPESLSKYDFNAVDLSGNGFEGDAFMFFGRNKTTVRVDLS 246

Query: 247 RNLLEFNMSKVVFPRTLTYLDLNHNKIFGEIPTEVVKLELQMFNVSYNALCGRIPMGGKL 306
           RN+  F++ KV F R++  LDL+ N I+G+IP  + KL L+ FNVS N LCG+IP GG L
Sbjct: 247 RNMFNFDLVKVKFARSIVSLDLSQNHIYGKIPPALTKLHLEHFNVSDNHLCGKIPSGGLL 306

Query: 307 QSFDVYSYFHNKCLCGKPLGSC 328
           Q+F+  ++ HN CLCG PL +C
Sbjct: 307 QTFEPSAFAHNICLCGTPLKAC 325

BLAST of Csa4G154320 vs. TAIR10
Match: AT5G12940.1 (AT5G12940.1 Leucine-rich repeat (LRR) family protein)

HSP 1 Score: 165.6 bits (418), Expect = 5.1e-41
Identity = 110/363 (30.30%), Postives = 167/363 (46.01%), Query Frame = 1

Query: 6   LLLLLLLLFFFTVSYAELCHPNDKKVLLNIKKAFNNPYI--LTSWKPEEDCCTWYCVECD 65
           +LL  +++F    +    C P+D+  LL  +   N PYI    +WK  + C  WY V CD
Sbjct: 9   ILLTNVVVFLLLSTTVHSCLPSDRAALLEFRAKLNEPYIGVFNTWKGLDCCKGWYGVSCD 68

Query: 66  RKSHRIIALTVFADDK------------LSGPIPPFVGDLPFLENLMFHKLPNLIGPIPP 125
             + R+  +T+  + +            ++G I P +  L  L  ++      + G IP 
Sbjct: 69  PNTRRVAGITLRGESEDPLFQKAKRSGLMTGSISPSICKLTRLSGIIIADWKGISGVIPS 128

Query: 126 TIAKLNNLKYLDLSWNGLSGPVPSFLGSLSNLDVLDLSFNRFTGSIPSSLANLRRLGTLH 185
            I  L  L++LDL  N  SG +P+ +G L  L VL+L+ N   G IP S+  L  L  L 
Sbjct: 129 CIENLPFLRHLDLVGNKFSGVIPANIGKLLRLKVLNLADNHLYGVIPPSITRLVSLSHLD 188

Query: 186 LDRNKLTGPIPESFGNFKGKVPYLYLSHNQLSGKIPISMGKV-DFNYIDLSRNKLVGDGS 245
           L  N ++G IP   G  K  V  + LS N++SG+IP S+ ++     ++LS N+L G   
Sbjct: 189 LRNNNISGVIPRDIGRLK-MVSRVLLSGNKISGQIPDSLTRIYRLADLELSMNRLTGPIP 248

Query: 246 LIFGSKKTTEIVDLSRNLLEFNMSKVVFPRTLTYLDLNHNKIFGEIPTEVVKLE------ 305
             FG       ++L  NL+   +   +   +++ L+L+ N I G IP             
Sbjct: 249 ASFGKMSVLATLNLDGNLISGMIPGSLLASSISNLNLSGNLITGSIPNTFGPRSYFTVLD 308

Query: 306 -------------------LQMFNVSYNALCGRIPMGGKLQSFDVYSYFHNKCLCGKPLG 329
                              +   +VS+N LCG+IPMG      D  S+ +N CLCGKPLG
Sbjct: 309 LANNRLQGPIPASITAASFIGHLDVSHNHLCGKIPMGSPFDHLDATSFAYNACLCGKPLG 368

BLAST of Csa4G154320 vs. TAIR10
Match: AT2G26380.1 (AT2G26380.1 Leucine-rich repeat (LRR) family protein)

HSP 1 Score: 158.7 bits (400), Expect = 6.2e-39
Identity = 112/314 (35.67%), Postives = 162/314 (51.59%), Query Frame = 1

Query: 2   HSPNLLLLLLLLFFFTVSYAELCHPNDKKVLLNIKKAFNNPY--ILTSWKPEEDCCTWYC 61
           H+  +   ++ L     + A  CHP+D+  LL  K         IL++WK   DCC+W  
Sbjct: 7   HNFFIFTAVIFLRCLNPTAAATCHPDDEAGLLAFKSGITKDPSGILSTWKKGTDCCSWNG 66

Query: 62  VECDRKSHRIIALTVF--ADDK---LSGPIPPFVGDLPFLENLMFHKLPNLIGPIPPTIA 121
           V C    +R++ LT+   +DD    LSG I P +  L  LE ++F  L N+ GP PP + 
Sbjct: 67  VSCPN-GNRVVVLTIRIESDDAGIFLSGTISPSLAKLQHLEGVVFINLKNITGPFPPFLF 126

Query: 122 KLNNLKYLDLSWNGLSGPVPSFLGSLSNLDVLDLSFNRFTGSIPSSLANLRRLGTLHLDR 181
           +L +LKY+ L    LSGP+P+ +G+L+ LD L +  NRF GSIPSS++NL RL  L+L  
Sbjct: 127 RLPHLKYVYLENTRLSGPLPANIGALNRLDTLTVKGNRFIGSIPSSISNLTRLNYLNLGG 186

Query: 182 NKLTGPIPESFGNFKGKVPYLYLSHNQLSGKIP-ISMGKVDFNYIDLSRNKLVGD-GSLI 241
           N LTG IP    N K  +  L L  N+LSG IP I     +   + LSRN+  G     I
Sbjct: 187 NLLTGTIPLGIANLK-LISNLNLDGNRLSGTIPDIFKSMTNLRILTLSRNRFSGKLPPSI 246

Query: 242 FGSKKTTEIVDLSRNLLEFN----MSKVVFPRTLTYLDLNHNKIFGEIPTEVVKL-ELQM 301
                    ++L +N L  +    +S+ V    L  LDL+ N+  G +P  + KL ++  
Sbjct: 247 ASLAPVLAFLELGQNNLSGSIPSYLSRFV---ALDTLDLSKNRFSGAVPKSLAKLTKIAN 306

HSP 2 Score: 130.6 bits (327), Expect = 1.8e-30
Identity = 94/279 (33.69%), Postives = 130/279 (46.59%), Query Frame = 1

Query: 78  DKLSGPIPPFVGDLPFLENLMFHKLPNLIGPIPPTIAKLNN-LKYLDLSWNGLSGPVPSF 137
           ++LSG IP     +  L  L   +     G +PP+IA L   L +L+L  N LSG +PS+
Sbjct: 210 NRLSGTIPDIFKSMTNLRILTLSR-NRFSGKLPPSIASLAPVLAFLELGQNNLSGSIPSY 269

Query: 138 LGSLSNLDVLDLSFNRFTGSIPSSLANLRRLGTLHLDRNKLTGPIPESFGNFKGKVPYLY 197
           L     LD LDLS NRF+G++P SLA L ++  ++L  N LT P P    N K  +  L 
Sbjct: 270 LSRFVALDTLDLSKNRFSGAVPKSLAKLTKIANINLSHNLLTNPFPVL--NVKNYILTLD 329

Query: 198 LSHNQLS-------------------GKIPISMGKVDFN--------YIDLSRNKLVGDG 257
           LS+N+                      K  I M   D+          IDLS N++ G  
Sbjct: 330 LSYNKFHMETIPEWVTSASILGSLKLAKCGIKMSLDDWKTRQTDLYVSIDLSDNEISGSP 389

Query: 258 SLIFGSKKTTEIVDLSRNLLEFNMSKVVFPRTLTYLDLNHNKIFGEIPTEVVKLELQMFN 317
                  +      +S N L F++ K+ F  TL  LDL+ N +FG++P  V    L+  N
Sbjct: 390 LRFLKGAEQLREFRMSGNKLRFDLRKLSFSTTLETLDLSRNLVFGKVPARVA--GLKTLN 449

Query: 318 VSYNALCGRIPMGGKLQSFDVYSYFHNKCLCGKPLGSCK 329
           +S N LCG++P    +  F    +  N CLCG PL  CK
Sbjct: 450 LSQNHLCGKLP----VTKFPESVFAGNDCLCGSPLSHCK 479

HSP 3 Score: 99.8 bits (247), Expect = 3.4e-21
Identity = 70/206 (33.98%), Postives = 106/206 (51.46%), Query Frame = 1

Query: 79  KLSGPIPPFVGDLPFLENLMFHKLPNLIGPIPPTIAKLNNLKYLDLSWNGLSGPVPSFLG 138
           +LSGP+P  +G L  L+ L   K    IG IP +I+ L  L YL+L  N L+G +P  + 
Sbjct: 139 RLSGPLPANIGALNRLDTLTV-KGNRFIGSIPSSISNLTRLNYLNLGGNLLTGTIPLGIA 198

Query: 139 SLSNLDVLDLSFNRFTGSIPSSLANLRRLGTLHLDRNKLTGPIPESFGNFKGKVPYLYLS 198
           +L  +  L+L  NR +G+IP    ++  L  L L RN+ +G +P S  +    + +L L 
Sbjct: 199 NLKLISNLNLDGNRLSGTIPDIFKSMTNLRILTLSRNRFSGKLPPSIASLAPVLAFLELG 258

Query: 199 HNQLSGKIPISMGK-VDFNYIDLSRNKLVGDGSLIFGSKKTTEI--VDLSRNLLEFNMSK 258
            N LSG IP  + + V  + +DLS+N+    G++     K T+I  ++LS NLL      
Sbjct: 259 QNNLSGSIPSYLSRFVALDTLDLSKNRF--SGAVPKSLAKLTKIANINLSHNLLTNPFPV 318

Query: 259 VVFPRTLTYLDLNHNKIFGEIPTEVV 282
           +     +  LDL++NK   E   E V
Sbjct: 319 LNVKNYILTLDLSYNKFHMETIPEWV 341

BLAST of Csa4G154320 vs. NCBI nr
Match: gi|700198674|gb|KGN53832.1| (Polygalacturonase-inhibiting protein [Cucumis sativus])

HSP 1 Score: 677.9 bits (1748), Expect = 8.6e-192
Identity = 328/328 (100.00%), Postives = 328/328 (100.00%), Query Frame = 1

Query: 1   MHSPNLLLLLLLLFFFTVSYAELCHPNDKKVLLNIKKAFNNPYILTSWKPEEDCCTWYCV 60
           MHSPNLLLLLLLLFFFTVSYAELCHPNDKKVLLNIKKAFNNPYILTSWKPEEDCCTWYCV
Sbjct: 1   MHSPNLLLLLLLLFFFTVSYAELCHPNDKKVLLNIKKAFNNPYILTSWKPEEDCCTWYCV 60

Query: 61  ECDRKSHRIIALTVFADDKLSGPIPPFVGDLPFLENLMFHKLPNLIGPIPPTIAKLNNLK 120
           ECDRKSHRIIALTVFADDKLSGPIPPFVGDLPFLENLMFHKLPNLIGPIPPTIAKLNNLK
Sbjct: 61  ECDRKSHRIIALTVFADDKLSGPIPPFVGDLPFLENLMFHKLPNLIGPIPPTIAKLNNLK 120

Query: 121 YLDLSWNGLSGPVPSFLGSLSNLDVLDLSFNRFTGSIPSSLANLRRLGTLHLDRNKLTGP 180
           YLDLSWNGLSGPVPSFLGSLSNLDVLDLSFNRFTGSIPSSLANLRRLGTLHLDRNKLTGP
Sbjct: 121 YLDLSWNGLSGPVPSFLGSLSNLDVLDLSFNRFTGSIPSSLANLRRLGTLHLDRNKLTGP 180

Query: 181 IPESFGNFKGKVPYLYLSHNQLSGKIPISMGKVDFNYIDLSRNKLVGDGSLIFGSKKTTE 240
           IPESFGNFKGKVPYLYLSHNQLSGKIPISMGKVDFNYIDLSRNKLVGDGSLIFGSKKTTE
Sbjct: 181 IPESFGNFKGKVPYLYLSHNQLSGKIPISMGKVDFNYIDLSRNKLVGDGSLIFGSKKTTE 240

Query: 241 IVDLSRNLLEFNMSKVVFPRTLTYLDLNHNKIFGEIPTEVVKLELQMFNVSYNALCGRIP 300
           IVDLSRNLLEFNMSKVVFPRTLTYLDLNHNKIFGEIPTEVVKLELQMFNVSYNALCGRIP
Sbjct: 241 IVDLSRNLLEFNMSKVVFPRTLTYLDLNHNKIFGEIPTEVVKLELQMFNVSYNALCGRIP 300

Query: 301 MGGKLQSFDVYSYFHNKCLCGKPLGSCK 329
           MGGKLQSFDVYSYFHNKCLCGKPLGSCK
Sbjct: 301 MGGKLQSFDVYSYFHNKCLCGKPLGSCK 328

BLAST of Csa4G154320 vs. NCBI nr
Match: gi|821595287|ref|NP_001295863.1| (polygalacturonase inhibitor-like precursor [Cucumis sativus])

HSP 1 Score: 677.6 bits (1747), Expect = 1.1e-191
Identity = 327/328 (99.70%), Postives = 328/328 (100.00%), Query Frame = 1

Query: 1   MHSPNLLLLLLLLFFFTVSYAELCHPNDKKVLLNIKKAFNNPYILTSWKPEEDCCTWYCV 60
           MHSPNLLLLLLLLFFFTVSYAELCHPNDKKVLLNIKKAFNNPYILTSWKPEEDCCTWYCV
Sbjct: 1   MHSPNLLLLLLLLFFFTVSYAELCHPNDKKVLLNIKKAFNNPYILTSWKPEEDCCTWYCV 60

Query: 61  ECDRKSHRIIALTVFADDKLSGPIPPFVGDLPFLENLMFHKLPNLIGPIPPTIAKLNNLK 120
           ECDRKSHRIIALTVFADDKLSGPIPPFVGDLPFLENLMFHKLPNLIGPIPPTIAKLNNLK
Sbjct: 61  ECDRKSHRIIALTVFADDKLSGPIPPFVGDLPFLENLMFHKLPNLIGPIPPTIAKLNNLK 120

Query: 121 YLDLSWNGLSGPVPSFLGSLSNLDVLDLSFNRFTGSIPSSLANLRRLGTLHLDRNKLTGP 180
           YLDLSWNGLSGP+PSFLGSLSNLDVLDLSFNRFTGSIPSSLANLRRLGTLHLDRNKLTGP
Sbjct: 121 YLDLSWNGLSGPIPSFLGSLSNLDVLDLSFNRFTGSIPSSLANLRRLGTLHLDRNKLTGP 180

Query: 181 IPESFGNFKGKVPYLYLSHNQLSGKIPISMGKVDFNYIDLSRNKLVGDGSLIFGSKKTTE 240
           IPESFGNFKGKVPYLYLSHNQLSGKIPISMGKVDFNYIDLSRNKLVGDGSLIFGSKKTTE
Sbjct: 181 IPESFGNFKGKVPYLYLSHNQLSGKIPISMGKVDFNYIDLSRNKLVGDGSLIFGSKKTTE 240

Query: 241 IVDLSRNLLEFNMSKVVFPRTLTYLDLNHNKIFGEIPTEVVKLELQMFNVSYNALCGRIP 300
           IVDLSRNLLEFNMSKVVFPRTLTYLDLNHNKIFGEIPTEVVKLELQMFNVSYNALCGRIP
Sbjct: 241 IVDLSRNLLEFNMSKVVFPRTLTYLDLNHNKIFGEIPTEVVKLELQMFNVSYNALCGRIP 300

Query: 301 MGGKLQSFDVYSYFHNKCLCGKPLGSCK 329
           MGGKLQSFDVYSYFHNKCLCGKPLGSCK
Sbjct: 301 MGGKLQSFDVYSYFHNKCLCGKPLGSCK 328

BLAST of Csa4G154320 vs. NCBI nr
Match: gi|940373614|ref|NP_001303611.1| (polygalacturonase inhibitor-like precursor [Cucumis melo])

HSP 1 Score: 641.0 bits (1652), Expect = 1.2e-180
Identity = 309/328 (94.21%), Postives = 319/328 (97.26%), Query Frame = 1

Query: 1   MHSPNLLLLLLLLFFFTVSYAELCHPNDKKVLLNIKKAFNNPYILTSWKPEEDCCTWYCV 60
           MHS NLLLLL   FFFTVS+AELCHPNDK+VLLNIKKAFNNPYILTSWKPEEDCCTWYCV
Sbjct: 1   MHSHNLLLLLF--FFFTVSFAELCHPNDKEVLLNIKKAFNNPYILTSWKPEEDCCTWYCV 60

Query: 61  ECDRKSHRIIALTVFADDKLSGPIPPFVGDLPFLENLMFHKLPNLIGPIPPTIAKLNNLK 120
           ECD KSHRIIALT+FADD+LSGPIPPFVGDLPFLENLMFHKLPNL GPIPPTIAKL+NLK
Sbjct: 61  ECDLKSHRIIALTIFADDELSGPIPPFVGDLPFLENLMFHKLPNLTGPIPPTIAKLHNLK 120

Query: 121 YLDLSWNGLSGPVPSFLGSLSNLDVLDLSFNRFTGSIPSSLANLRRLGTLHLDRNKLTGP 180
           YLDLSWNGLSGP+PSFLGSLSNLD+LDLSFNRFTGSIPSSLANLRRLGTLHLDRNKLTGP
Sbjct: 121 YLDLSWNGLSGPIPSFLGSLSNLDILDLSFNRFTGSIPSSLANLRRLGTLHLDRNKLTGP 180

Query: 181 IPESFGNFKGKVPYLYLSHNQLSGKIPISMGKVDFNYIDLSRNKLVGDGSLIFGSKKTTE 240
           IP+SFGNFKGKVPYLYLSHNQLSGKIP SMGKVDFNYIDLSRNKLVGDGSLIFGSKKTTE
Sbjct: 181 IPDSFGNFKGKVPYLYLSHNQLSGKIPTSMGKVDFNYIDLSRNKLVGDGSLIFGSKKTTE 240

Query: 241 IVDLSRNLLEFNMSKVVFPRTLTYLDLNHNKIFGEIPTEVVKLELQMFNVSYNALCGRIP 300
           IVDLSRNLLEFN+SKVVFPRTLT+LDLNHNKIFGEIPT VVKLELQM NVSYNALCGRIP
Sbjct: 241 IVDLSRNLLEFNISKVVFPRTLTWLDLNHNKIFGEIPTGVVKLELQMLNVSYNALCGRIP 300

Query: 301 MGGKLQSFDVYSYFHNKCLCGKPLGSCK 329
           MGGKLQSFDVYSYFHNKCLCGKPLGSCK
Sbjct: 301 MGGKLQSFDVYSYFHNKCLCGKPLGSCK 326

BLAST of Csa4G154320 vs. NCBI nr
Match: gi|425904953|gb|AFY10521.1| (polygalacturonase-inhibiting protein [Cucumis melo subsp. agrestis])

HSP 1 Score: 639.0 bits (1647), Expect = 4.4e-180
Identity = 308/328 (93.90%), Postives = 318/328 (96.95%), Query Frame = 1

Query: 1   MHSPNLLLLLLLLFFFTVSYAELCHPNDKKVLLNIKKAFNNPYILTSWKPEEDCCTWYCV 60
           MHS NLLLLL   FFFTVS+AELCHPNDK+VLLNIKKAFNNPYILTSWKPEEDCCTWYCV
Sbjct: 1   MHSHNLLLLLF--FFFTVSFAELCHPNDKEVLLNIKKAFNNPYILTSWKPEEDCCTWYCV 60

Query: 61  ECDRKSHRIIALTVFADDKLSGPIPPFVGDLPFLENLMFHKLPNLIGPIPPTIAKLNNLK 120
           ECD KSHRI ALT+FADD+LSGPIPPFVGDLPFLENLMFHKLPNL GPIPPTIAKL+NLK
Sbjct: 61  ECDLKSHRITALTIFADDELSGPIPPFVGDLPFLENLMFHKLPNLTGPIPPTIAKLHNLK 120

Query: 121 YLDLSWNGLSGPVPSFLGSLSNLDVLDLSFNRFTGSIPSSLANLRRLGTLHLDRNKLTGP 180
           YLDLSWNGLSGP+PSFLGSLSNLD+LDLSFNRFTGSIPSSLANLRRLGTLHLDRNKLTGP
Sbjct: 121 YLDLSWNGLSGPIPSFLGSLSNLDILDLSFNRFTGSIPSSLANLRRLGTLHLDRNKLTGP 180

Query: 181 IPESFGNFKGKVPYLYLSHNQLSGKIPISMGKVDFNYIDLSRNKLVGDGSLIFGSKKTTE 240
           IP+SFGNFKGKVPYLYLSHNQLSGKIP SMGKVDFNYIDLSRNKLVGDGSLIFGSKKTTE
Sbjct: 181 IPDSFGNFKGKVPYLYLSHNQLSGKIPTSMGKVDFNYIDLSRNKLVGDGSLIFGSKKTTE 240

Query: 241 IVDLSRNLLEFNMSKVVFPRTLTYLDLNHNKIFGEIPTEVVKLELQMFNVSYNALCGRIP 300
           IVDLSRNLLEFN+SKVVFPRTLT+LDLNHNKIFGEIPT VVKLELQM NVSYNALCGRIP
Sbjct: 241 IVDLSRNLLEFNISKVVFPRTLTWLDLNHNKIFGEIPTGVVKLELQMLNVSYNALCGRIP 300

Query: 301 MGGKLQSFDVYSYFHNKCLCGKPLGSCK 329
           MGGKLQSFDVYSYFHNKCLCGKPLGSCK
Sbjct: 301 MGGKLQSFDVYSYFHNKCLCGKPLGSCK 326

BLAST of Csa4G154320 vs. NCBI nr
Match: gi|659102348|ref|XP_008452081.1| (PREDICTED: polygalacturonase inhibitor-like [Cucumis melo])

HSP 1 Score: 469.9 bits (1208), Expect = 3.6e-129
Identity = 222/326 (68.10%), Postives = 263/326 (80.67%), Query Frame = 1

Query: 4   PNLLLLLLLLFFFTVSYAELCHPNDKKVLLNIKKAFNNPYILTSWKPEEDCCTWYCVECD 63
           P   +     FF T+S+AELC+PNDKKVLL IKKAFNNPYILTSW P+ DCC WYCV+CD
Sbjct: 12  PFFFIFFFFFFFITISFAELCNPNDKKVLLKIKKAFNNPYILTSWDPQTDCCHWYCVKCD 71

Query: 64  RKSHRIIALTVFADDKLSGPIPPFVGDLPFLENLMFHKLPNLIGPIPPTIAKLNNLKYLD 123
           R +HRII+LT+FADD+L+G IPP VGDLPFL+ LM HKLPNL GPI PTIAKL+NLK+LD
Sbjct: 72  RTTHRIISLTIFADDRLTGQIPPEVGDLPFLQTLMLHKLPNLTGPIQPTIAKLHNLKFLD 131

Query: 124 LSWNGLSGPVPSFLGSLSNLDVLDLSFNRFTGSIPSSLANLRRLGTLHLDRNKLTGPIPE 183
           LSWNGLSG +P  L +L NL +L LSFN+ TG IPSSL+ L  LG L LDRN+LTG IP+
Sbjct: 132 LSWNGLSGEIPDSLSTLKNLFILTLSFNKLTGKIPSSLSELPSLGGLRLDRNQLTGQIPD 191

Query: 184 SFGNFKG-KVPYLYLSHNQLSGKIPISMGKVDFNYIDLSRNKLVGDGSLIFGSKKTTEIV 243
           SFG F G + P L LSHNQLSGKIP S+ +++F  IDLSRNKL GD  + FG+ KT +I+
Sbjct: 192 SFGYFAGEQAPDLILSHNQLSGKIPSSLSRLNFTSIDLSRNKLEGDAFMFFGANKTIQIL 251

Query: 244 DLSRNLLEFNMSKVVFPRTLTYLDLNHNKIFGEIPTEVVKLELQMFNVSYNALCGRIPMG 303
           DLSRNLLEFN+S+VVFP++LT LDLNHNK+FG +P  + +L+LQ  NVSYN LCG IP+G
Sbjct: 252 DLSRNLLEFNLSEVVFPQSLTSLDLNHNKVFGGLPPVMTELKLQYLNVSYNRLCGPIPVG 311

Query: 304 GKLQSFDVYSYFHNKCLCGKPLGSCK 329
           G LQSFD+YSYFHNKCLCG PLG CK
Sbjct: 312 GNLQSFDLYSYFHNKCLCGAPLGDCK 337