Csa4G103350 (gene) Cucumber (Chinese Long) v2

BLAST of Csa4G103350 vs. Swiss-Prot
Match: DIM_PEA (Delta(24)-sterol reductase OS=Pisum sativum GN=DIM PE=1 SV=1)

HSP 1 Score: 945.7 bits (2443), Expect = 2.4e-274
Identity = 445/564 (78.90%), Postives = 500/564 (88.65%), Query Frame = 1

Query: 1   MTDLEASKPQKRKKGWVDILVNFRWIIVIFVVLPFSFSFYFIQYLGDKRSERKSYKQRQI 60
           M+DLEA    KRKK WVD  V FRWI+VIFVVLP SF+ YF+ YLGD RSE KS+K RQ 
Sbjct: 1   MSDLEAPLRPKRKKIWVDYFVKFRWILVIFVVLPISFTLYFLTYLGDVRSEWKSFKTRQK 60

Query: 61  EHEENVQKVVERLKQRNPSKDGLVCTARKPWLAVGMRNVDYKRARHFEVDLSAFRNILDI 120
           EH+ENVQKVV RLK+RNPSKDGLVCTARKPW+AVGMRNVDYKRARHFEVDLS FRNILDI
Sbjct: 61  EHDENVQKVVNRLKKRNPSKDGLVCTARKPWVAVGMRNVDYKRARHFEVDLSPFRNILDI 120

Query: 121 DKERMIAKVEPHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS 180
           DKERMIA+VEP VNMGQI+R T+PMNL+LAVVAELDDLTVGGLINGYGIEGSSH YGLFS
Sbjct: 121 DKERMIARVEPLVNMGQITRVTVPMNLALAVVAELDDLTVGGLINGYGIEGSSHKYGLFS 180

Query: 181 DTVVAYEIVLADGRVVRATKDNEYSDLFYAIPWSQGTLGFLVSAEIKLIHIKEYMKLTYL 240
           DTVVA+EI+LADG +V+ATKDNEYSDLFYAIPWSQGTLG LV+AE+KLI IKEYMKLTY 
Sbjct: 181 DTVVAFEIILADGSLVKATKDNEYSDLFYAIPWSQGTLGLLVAAEVKLIPIKEYMKLTYK 240

Query: 241 PVKGNLQEIGQAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYASKEEAKKK 300
           PV GNL++I QAY DSF PRD DQDN EKVPDFVETMIYSPT AVCMTGRYASKEEAKKK
Sbjct: 241 PVVGNLKDIAQAYSDSFAPRDGDQDNDEKVPDFVETMIYSPTRAVCMTGRYASKEEAKKK 300

Query: 301 GNVINNIGWWFKPWFYQHAQKSLKNGEFVEYIPTREYYHRHTKSLYWEGKLILPFADQWW 360
           GN INN+GWW+K WFYQHA+ +LK G FVEYIPTREYYHRHT+ LYWEGKLILPF DQ+ 
Sbjct: 301 GNKINNVGWWYKTWFYQHAETALKKGLFVEYIPTREYYHRHTRCLYWEGKLILPFGDQFS 360

Query: 361 FRFFLGWMMPPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWIHREMEVYPL 420
           FRF  GW+MPPKVSLLKATQGEAIRNYYHEMH++QDMLVPL ++GD LEW  REME+YP+
Sbjct: 361 FRFLFGWLMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVGDALEWSDREMEIYPI 420

Query: 421 WLCPHRLFKPPMKTMVSPEPGFELQLKQGDTPYAQMYTDIGVYYAPGPVLRGEVYDGVQA 480
           WLCPH+LFK P+KTM+ PE GFELQ +QGDT  AQM+TD+GVYYAPGPVLRGEV+DG +A
Sbjct: 421 WLCPHKLFKLPIKTMIYPEAGFELQRRQGDTQNAQMFTDVGVYYAPGPVLRGEVFDGAEA 480

Query: 481 VRNMENWLIEIHGFQPQHSVTEMSEKEFWKMFDSELYEACRKKYGAIGTFMHVYYKCKKG 540
           VR ME+W+IE H FQPQ++V+E++EK FW+MFD+ LYE CR+KYGA+GTFM VYYKCKKG
Sbjct: 481 VRKMESWMIENHCFQPQYAVSELNEKNFWRMFDAGLYEHCRRKYGAVGTFMSVYYKCKKG 540

Query: 541 RKTEKEVEEAEKAQAETAYADVEQ 565
           RKTEKEV EAE+A  +TAY +V+Q
Sbjct: 541 RKTEKEVREAEQAHLDTAYPEVDQ 564

BLAST of Csa4G103350 vs. Swiss-Prot
Match: DIM_ARATH (Delta(24)-sterol reductase OS=Arabidopsis thaliana GN=DIM PE=1 SV=2)

HSP 1 Score: 903.3 bits (2333), Expect = 1.4e-261
Identity = 423/564 (75.00%), Postives = 489/564 (86.70%), Query Frame = 1

Query: 1   MTDLEASKPQ-KRKKGWVDILVNFRWIIVIFVVLPFSFSFYFIQYLGDKRSERKSYKQRQ 60
           M+DL+    + KRKK WVD  V FRWIIVIF+VLPFS +FYF+ YLGD  SE KS+++RQ
Sbjct: 1   MSDLQTPLVRPKRKKTWVDYFVKFRWIIVIFIVLPFSATFYFLIYLGDMWSESKSFEKRQ 60

Query: 61  IEHEENVQKVVERLKQRNPSKDGLVCTARKPWLAVGMRNVDYKRARHFEVDLSAFRNILD 120
            EH+ENV+KV++RLK R+ SKDGLVCTARKPW+AVGMRNVDYKRARHFEVDL  FRNIL+
Sbjct: 61  KEHDENVKKVIKRLKGRDASKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLGEFRNILE 120

Query: 121 IDKERMIAKVEPHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLF 180
           I+KE+M A+VEP VNMGQISRAT+PMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLF
Sbjct: 121 INKEKMTARVEPLVNMGQISRATVPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLF 180

Query: 181 SDTVVAYEIVLADGRVVRATKDNEYSDLFYAIPWSQGTLGFLVSAEIKLIHIKEYMKLTY 240
           +DTV AYEIVLA G +VRAT+DNEYSDL+YAIPWSQGTLG LV+AEI+LI +KEYM+LTY
Sbjct: 181 ADTVEAYEIVLAGGELVRATRDNEYSDLYYAIPWSQGTLGLLVAAEIRLIKVKEYMRLTY 240

Query: 241 LPVKGNLQEIGQAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYASKEEAKK 300
           +PVKG+LQ + Q Y+DSF P+D D+    K+PDFVE M+Y+PTE V M G YASKEEAKK
Sbjct: 241 IPVKGDLQALAQGYIDSFAPKDGDKS---KIPDFVEGMVYNPTEGVMMVGTYASKEEAKK 300

Query: 301 KGNVINNIGWWFKPWFYQHAQKSLKNGEFVEYIPTREYYHRHTKSLYWEGKLILPFADQW 360
           KGN INN+GWWFKPWFYQHAQ +LK G+FVEYIPTREYYHRHT+ LYWEGKLILPF DQ+
Sbjct: 301 KGNKINNVGWWFKPWFYQHAQTALKKGQFVEYIPTREYYHRHTRCLYWEGKLILPFGDQF 360

Query: 361 WFRFFLGWMMPPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWIHREMEVYP 420
           WFR+ LGW+MPPKVSLLKATQGEAIRNYYH+MH++QDMLVPL ++GD LEW+HREMEVYP
Sbjct: 361 WFRYLLGWLMPPKVSLLKATQGEAIRNYYHDMHVIQDMLVPLYKVGDALEWVHREMEVYP 420

Query: 421 LWLCPHRLFKPPMKTMVSPEPGFELQLKQGDTPYAQMYTDIGVYYAPGPVLRGEVYDGVQ 480
           +WLCPH+LFK P+K  + PEPGFE + +QGDT  AQMYTD+GVYYAPG VLRGE +DG +
Sbjct: 421 IWLCPHKLFKQPIKGQIYPEPGFEYENRQGDTEDAQMYTDVGVYYAPGCVLRGEEFDGSE 480

Query: 481 AVRNMENWLIEIHGFQPQHSVTEMSEKEFWKMFDSELYEACRKKYGAIGTFMHVYYKCKK 540
           AVR ME WLIE HGFQPQ++V+E+ EK FW+MF+ ELYE CRKKY AIGTFM VYYK KK
Sbjct: 481 AVRRMEKWLIENHGFQPQYAVSELDEKSFWRMFNGELYEECRKKYRAIGTFMSVYYKSKK 540

Query: 541 GRKTEKEVEEAEKAQAETAYADVE 564
           GRKTEKEV EAE+A  ETAYA+ +
Sbjct: 541 GRKTEKEVREAEQAHLETAYAEAD 561

BLAST of Csa4G103350 vs. Swiss-Prot
Match: DHC24_RAT (Delta(24)-sterol reductase OS=Rattus norvegicus GN=Dhcr24 PE=2 SV=2)

HSP 1 Score: 381.7 bits (979), Expect = 1.4e-104
Identity = 220/533 (41.28%), Postives = 311/533 (58.35%), Query Frame = 1

Query: 12  RKKGWVDILVNFRWIIVIFVVLPFSFSFYFIQYLGDKRSERKSYKQRQIEHEENVQKVVE 71
           R KG   +L++ RW+ V   +LP S  F    Y+      + S   R   HE+ VQ + +
Sbjct: 19  RVKGLEFVLIHQRWVFVCLFLLPLSLIFDIYYYVRAWVVFKLSSAPRL--HEQRVQDIQK 78

Query: 72  RLKQ-RNPSKDGLVCTARKPWLAVGMRNVDYKRARHFEVDLSAFRNILDIDKERMIAKVE 131
           ++++ +       +CT R  WL V +R   YK+  H  + ++   +IL++D ++ I +VE
Sbjct: 79  QVREWKEQGSKTFMCTGRPGWLTVSLRVGKYKKT-HKNIMINLM-DILEVDTKKQIVRVE 138

Query: 132 PHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFSDTVVAYEIVL 191
           P V+MGQ++     +  +L V+ ELDDLTVGGLI G GIE SSH YGLF     AYE++L
Sbjct: 139 PLVSMGQVTALLNSIGWTLPVLPELDDLTVGGLIMGTGIESSSHKYGLFQHICTAYELIL 198

Query: 192 ADGRVVRATKDNEYSDLFYAIPWSQGTLGFLVSAEIKLIHIKEYMKLTYLPVKGNLQEIG 251
           ADG  VR T  +E SDLFYA+PWS GTLGFLV+AEI++I  K+Y+KL + PV+G      
Sbjct: 199 ADGSFVRCTP-SENSDLFYAVPWSCGTLGFLVAAEIRIIPAKKYVKLRFEPVRGL----- 258

Query: 252 QAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYASKEEAKKKGNVINNIGWW 311
           +A  + FT      +N      FVE ++YS  EAV MTG      E  K    +N+IG +
Sbjct: 259 EAICEKFTHESQRLEN-----HFVEGLLYSLDEAVIMTGVMTDDVEPSK----LNSIGSY 318

Query: 312 FKPWFYQHAQKSLK-NGEFVEYIPTREYYHRHTKSLYWEGKLILPFADQWWFRFFLGWMM 371
           +KPWF++H +  LK N E +EYIP R YYHRHT+S++WE + I+PF +   FR+  GWM+
Sbjct: 319 YKPWFFKHVENYLKTNREGLEYIPLRHYYHRHTRSIFWELQDIIPFGNNPIFRYLFGWMV 378

Query: 372 PPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWIHREMEVYPLWLCPHRLFK 431
           PPK+SLLK TQGE +R  Y + H+VQDMLVP++ +   L     ++ VYP+WLCP  L  
Sbjct: 379 PPKISLLKLTQGETLRKLYEQHHVVQDMLVPMKCLSQALHTFQNDIHVYPIWLCPFIL-- 438

Query: 432 PPMKTMVSPEPGFELQLKQGDTPYAQMYTDIGVYYAPGPVLRGEVYDGVQAVRNMENWLI 491
           P    +V P         +GD   A++Y DIG Y  P    R + ++    +R +E ++ 
Sbjct: 439 PSQPGLVHP---------KGDE--AELYVDIGAYGEP----RVKHFEARSCMRQLEKFVR 498

Query: 492 EIHGFQPQHSVTEMSEKEFWKMFDSELYEACRKKYGAIGTFMHVYYK-CKKGR 542
            +HGFQ  ++   M+ +EFW+MFD  LY   RK+ G    F  VY K CK  R
Sbjct: 499 SVHGFQMLYADCYMNREEFWEMFDGSLYHKLRKQLGCQDAFPEVYDKICKAAR 515

BLAST of Csa4G103350 vs. Swiss-Prot
Match: DHC24_HUMAN (Delta(24)-sterol reductase OS=Homo sapiens GN=DHCR24 PE=1 SV=2)

HSP 1 Score: 380.6 bits (976), Expect = 3.1e-104
Identity = 221/533 (41.46%), Postives = 311/533 (58.35%), Query Frame = 1

Query: 12  RKKGWVDILVNFRWIIVIFVVLPFSFSFYFIQYLGDKRSERKSYKQRQIEHEENVQKVVE 71
           R KG   +L++ RW+ V   +LP S  F    Y+      + S   R   HE+ V+ + +
Sbjct: 19  RLKGLEFVLIHQRWVFVCLFLLPLSLIFDIYYYVRAWVVFKLSSAPRL--HEQRVRDIQK 78

Query: 72  RLKQ-RNPSKDGLVCTARKPWLAVGMRNVDYKRARHFEVDLSAFRNILDIDKERMIAKVE 131
           ++++ +       +CT R  WL V +R   YK+  H  + ++   +IL++D ++ I +VE
Sbjct: 79  QVREWKEQGSKTFMCTGRPGWLTVSLRVGKYKKT-HKNIMINLM-DILEVDTKKQIVRVE 138

Query: 132 PHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFSDTVVAYEIVL 191
           P V MGQ++     +  +L V+ ELDDLTVGGLI G GIE SSH YGLF     AYE+VL
Sbjct: 139 PLVTMGQVTALLTSIGWTLPVLPELDDLTVGGLIMGTGIESSSHKYGLFQHICTAYELVL 198

Query: 192 ADGRVVRATKDNEYSDLFYAIPWSQGTLGFLVSAEIKLIHIKEYMKLTYLPVKGNLQEIG 251
           ADG  VR T  +E SDLFYA+PWS GTLGFLV+AEI++I  K+Y+KL + PV+G      
Sbjct: 199 ADGSFVRCTP-SENSDLFYAVPWSCGTLGFLVAAEIRIIPAKKYVKLRFEPVRGL----- 258

Query: 252 QAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYASKEEAKKKGNVINNIGWW 311
           +A    FT     Q+N      FVE ++YS  EAV MTG    + E  K    +N+IG +
Sbjct: 259 EAICAKFTHESQRQEN-----HFVEGLLYSLDEAVIMTGVMTDEAEPSK----LNSIGNY 318

Query: 312 FKPWFYQHAQKSLK-NGEFVEYIPTREYYHRHTKSLYWEGKLILPFADQWWFRFFLGWMM 371
           +KPWF++H +  LK N E +EYIP R YYHRHT+S++WE + I+PF +   FR+  GWM+
Sbjct: 319 YKPWFFKHVENYLKTNREGLEYIPLRHYYHRHTRSIFWELQDIIPFGNNPIFRYLFGWMV 378

Query: 372 PPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWIHREMEVYPLWLCPHRLFK 431
           PPK+SLLK TQGE +R  Y + H+VQDMLVP++ +   L     ++ VYP+WLCP  L  
Sbjct: 379 PPKISLLKLTQGETLRKLYEQHHVVQDMLVPMKCLQQALHTFQNDIHVYPIWLCPFIL-- 438

Query: 432 PPMKTMVSPEPGFELQLKQGDTPYAQMYTDIGVYYAPGPVLRGEVYDGVQAVRNMENWLI 491
           P    +V P+ G E          A++Y DIG Y  P    R + ++    +R +E ++ 
Sbjct: 439 PSQPGLVHPK-GNE----------AELYIDIGAYGEP----RVKHFEARSCMRQLEKFVR 498

Query: 492 EIHGFQPQHSVTEMSEKEFWKMFDSELYEACRKKYGAIGTFMHVYYK-CKKGR 542
            +HGFQ  ++   M+ +EFW+MFD  LY   R+K G    F  VY K CK  R
Sbjct: 499 SVHGFQMLYADCYMNREEFWEMFDGSLYHKLREKLGCQDAFPEVYDKICKAAR 515

BLAST of Csa4G103350 vs. Swiss-Prot
Match: DHC24_MOUSE (Delta(24)-sterol reductase OS=Mus musculus GN=Dhcr24 PE=1 SV=1)

HSP 1 Score: 379.8 bits (974), Expect = 5.2e-104
Identity = 219/533 (41.09%), Postives = 311/533 (58.35%), Query Frame = 1

Query: 12  RKKGWVDILVNFRWIIVIFVVLPFSFSFYFIQYLGDKRSERKSYKQRQIEHEENVQKVVE 71
           R KG   +L++ RW+ V   +LP S  F    Y+      + S   R   HE+ V+ + +
Sbjct: 19  RVKGLEFVLIHQRWVFVCLFLLPLSLIFDIYYYVRAWVVFKLSSAPRL--HEQRVRDIQK 78

Query: 72  RLKQ-RNPSKDGLVCTARKPWLAVGMRNVDYKRARHFEVDLSAFRNILDIDKERMIAKVE 131
           ++++ +       +CT R  WL V +R   YK+  H  + ++   +IL++D ++ I +VE
Sbjct: 79  QVREWKEQGSKTFMCTGRPGWLTVSLRVGKYKKT-HKNIMINLM-DILEVDTKKQIVRVE 138

Query: 132 PHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFSDTVVAYEIVL 191
           P V+MGQ++     +  +L V+ ELDDLTVGGLI G GIE SSH YGLF     AYE++L
Sbjct: 139 PLVSMGQVTALLNSIGWTLPVLPELDDLTVGGLIMGTGIESSSHKYGLFQHICTAYELIL 198

Query: 192 ADGRVVRATKDNEYSDLFYAIPWSQGTLGFLVSAEIKLIHIKEYMKLTYLPVKGNLQEIG 251
           ADG  VR T  +E SDLFYA+PWS GTLGFLV+AEI++I  K+Y+KL + PV+G      
Sbjct: 199 ADGSFVRCTP-SENSDLFYAVPWSCGTLGFLVAAEIRIIPAKKYVKLRFEPVRGL----- 258

Query: 252 QAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYASKEEAKKKGNVINNIGWW 311
           +A  + FT      +N      FVE ++YS  EAV MTG      E  K    +N+IG +
Sbjct: 259 EAICEKFTRESQRLEN-----HFVEGLLYSLDEAVIMTGVMTDDVEPSK----LNSIGSY 318

Query: 312 FKPWFYQHAQKSLK-NGEFVEYIPTREYYHRHTKSLYWEGKLILPFADQWWFRFFLGWMM 371
           +KPWF++H +  LK N E +EYIP R YYHRHT+S++WE + I+PF +   FR+  GWM+
Sbjct: 319 YKPWFFKHVENYLKTNREGLEYIPLRHYYHRHTRSIFWELQDIIPFGNNPIFRYLFGWMV 378

Query: 372 PPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWIHREMEVYPLWLCPHRLFK 431
           PPK+SLLK TQGE +R  Y + H+VQDMLVP++ +   L     ++ VYP+WLCP  L  
Sbjct: 379 PPKISLLKLTQGETLRKLYEQHHVVQDMLVPMKCMSQALHTFQNDIHVYPIWLCPFIL-- 438

Query: 432 PPMKTMVSPEPGFELQLKQGDTPYAQMYTDIGVYYAPGPVLRGEVYDGVQAVRNMENWLI 491
           P    +V P         +GD   A++Y DIG Y  P    R + ++    +R +E ++ 
Sbjct: 439 PSQPGLVHP---------KGDE--AELYVDIGAYGEP----RVKHFEARSCMRQLEKFVR 498

Query: 492 EIHGFQPQHSVTEMSEKEFWKMFDSELYEACRKKYGAIGTFMHVYYK-CKKGR 542
            +HGFQ  ++   M+ +EFW+MFD  LY   RK+ G    F  VY K CK  R
Sbjct: 499 SVHGFQMLYADCYMNREEFWEMFDGSLYHKLRKQLGCQDAFPEVYDKICKAAR 515

BLAST of Csa4G103350 vs. TrEMBL
Match: W9SYN1_9ROSA (Delta(24)-sterol reductase OS=Morus notabilis GN=L484_014984 PE=4 SV=1)

HSP 1 Score: 999.6 bits (2583), Expect = 1.6e-288
Identity = 466/567 (82.19%), Postives = 520/567 (91.71%), Query Frame = 1

Query: 1   MTDLEASKPQKRKKGWVDILVNFRWIIVIFVVLPFSFSFYFIQYLGDKRSERKSYKQRQI 60
           M+DLEA    KRKK WVD  V FRWI+VIFVVLP SF+ YF+ YLGD +SERKS+KQRQ 
Sbjct: 1   MSDLEAPLRPKRKKIWVDYFVQFRWILVIFVVLPISFTLYFLTYLGDVKSERKSFKQRQK 60

Query: 61  EHEENVQKVVERLKQRNPSKDGLVCTARKPWLAVGMRNVDYKRARHFEVDLSAFRNILDI 120
           EH+ENVQKV++RLKQR+PSKDGLVCTARKPW+AVGMRNVDYKRARHFEVDLSAFRNILDI
Sbjct: 61  EHDENVQKVIKRLKQRDPSKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI 120

Query: 121 DKERMIAKVEPHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS 180
           DK+RMIA+VEP VNMGQISRAT+PMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS
Sbjct: 121 DKDRMIARVEPLVNMGQISRATVPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS 180

Query: 181 DTVVAYEIVLADGRVVRATKDNEYSDLFYAIPWSQGTLGFLVSAEIKLIHIKEYMKLTYL 240
           DTVVAYEIVLADGRVVRATKDNE+SDLFYAIPWSQGTLG LV+AEIKLI +KEYM++TY 
Sbjct: 181 DTVVAYEIVLADGRVVRATKDNEFSDLFYAIPWSQGTLGLLVAAEIKLIPVKEYMRVTYK 240

Query: 241 PVKGNLQEIGQAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYASKEEAKKK 300
           PV GNLQE+GQ Y+DSF PRD DQDNP+KVPDFVETMIY+P EAVCMTGRYASKEEAKKK
Sbjct: 241 PVVGNLQELGQGYIDSFAPRDCDQDNPDKVPDFVETMIYNPAEAVCMTGRYASKEEAKKK 300

Query: 301 GNVINNIGWWFKPWFYQHAQKSLKNGEFVEYIPTREYYHRHTKSLYWEGKLILPFADQWW 360
           GNVIN++GWWFKPWFYQHAQ +LK GEFVEYIPTREYYHRHT+ LYWEGKLILPFADQWW
Sbjct: 301 GNVINSVGWWFKPWFYQHAQTALKKGEFVEYIPTREYYHRHTRCLYWEGKLILPFADQWW 360

Query: 361 FRFFLGWMMPPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWIHREMEVYPL 420
           FRF LGWMMPPKVSLLKATQGEAIRNYYHEMH++QDMLVPL ++GD LEW+H+EMEVYP+
Sbjct: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVGDALEWVHQEMEVYPI 420

Query: 421 WLCPHRLFKPPMKTMVSPEPGFELQLKQGDTPYAQMYTDIGVYYAPGPVLRGEVYDGVQA 480
           WLCPHRL+K P+KTM+ PEPGFEL  +QGDT YAQMYTD+GVYYAPGPVLRGEV+DG  A
Sbjct: 421 WLCPHRLYKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGVYYAPGPVLRGEVFDGAGA 480

Query: 481 VRNMENWLIEIHGFQPQHSVTEMSEKEFWKMFDSELYEACRKKYGAIGTFMHVYYKCKKG 540
           VR ME+WLI+ HGFQPQ++V+E+SEK FW+MFD+ LYE CR+KYGA+GTFM VYYK KKG
Sbjct: 481 VRKMEDWLIKNHGFQPQYAVSELSEKSFWRMFDAGLYEHCRRKYGAVGTFMSVYYKSKKG 540

Query: 541 RKTEKEVEEAEKAQAETAYADVEQAGE 568
           RKTEKEV EAE+A  ETAYA+V+Q  +
Sbjct: 541 RKTEKEVREAEQAHLETAYAEVDQPAD 567

BLAST of Csa4G103350 vs. TrEMBL
Match: B9HHN5_POPTR (Diminuto family protein OS=Populus trichocarpa GN=POPTR_0008s08440g PE=4 SV=1)

HSP 1 Score: 992.3 bits (2564), Expect = 2.5e-286
Identity = 469/561 (83.60%), Postives = 514/561 (91.62%), Query Frame = 1

Query: 1   MTDLEASKPQKRKKGWVDILVNFRWIIVIFVVLPFSFSFYFIQYLGDKRSERKSYKQRQI 60
           M+DLEA    KRKK WVD  V FRWI+VIFVVLP SF+ YF+ YLGD +SE KSYKQRQ 
Sbjct: 1   MSDLEAPLRPKRKKVWVDYFVQFRWILVIFVVLPISFTLYFLTYLGDVKSEMKSYKQRQK 60

Query: 61  EHEENVQKVVERLKQRNPSKDGLVCTARKPWLAVGMRNVDYKRARHFEVDLSAFRNILDI 120
           EH+ENV+KVV+RLK+RNPSKDGLVCTARKPW+AVGMRNVDYKRARHFEVDLSAFRNIL+I
Sbjct: 61  EHDENVKKVVKRLKERNPSKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILEI 120

Query: 121 DKERMIAKVEPHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS 180
           DKERMIA+VEP VNMGQISRA++PMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS
Sbjct: 121 DKERMIARVEPLVNMGQISRASVPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS 180

Query: 181 DTVVAYEIVLADGRVVRATKDNEYSDLFYAIPWSQGTLGFLVSAEIKLIHIKEYMKLTYL 240
           DTVVAYEIVLADG+VVRATKDNEYSDLFYAIPWSQGTLG LVSAEIKLI IKEYM+LTY 
Sbjct: 181 DTVVAYEIVLADGQVVRATKDNEYSDLFYAIPWSQGTLGLLVSAEIKLIPIKEYMRLTYK 240

Query: 241 PVKGNLQEIGQAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYASKEEAKKK 300
           PV GNL+E+ QAY+DSF PRD DQDNPEKVPDFVETMIY+ TE V MTGRYASKEEAKKK
Sbjct: 241 PVVGNLKELAQAYIDSFAPRDGDQDNPEKVPDFVETMIYNSTEGVMMTGRYASKEEAKKK 300

Query: 301 GNVINNIGWWFKPWFYQHAQKSLKNGEFVEYIPTREYYHRHTKSLYWEGKLILPFADQWW 360
           GNVINN+GWWFKPWFYQHAQ +LK GEFVEYIPTREYYHRHT+ LYWEGKLILPFADQWW
Sbjct: 301 GNVINNVGWWFKPWFYQHAQTALKKGEFVEYIPTREYYHRHTRCLYWEGKLILPFADQWW 360

Query: 361 FRFFLGWMMPPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWIHREMEVYPL 420
           FRF LGWMMPPKVSLLKATQGEAIRNYYHEMH++QDMLVPL ++GD LEW+H+EMEVYPL
Sbjct: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVGDALEWVHQEMEVYPL 420

Query: 421 WLCPHRLFKPPMKTMVSPEPGFELQLKQGDTPYAQMYTDIGVYYAPGPVLRGEVYDGVQA 480
           WLCPHRLFK P+KTMV PEPGFE Q KQGDT YAQMYTD+GVYY+PGPVLRGEV+DG  A
Sbjct: 421 WLCPHRLFKLPVKTMVYPEPGFEHQHKQGDTSYAQMYTDVGVYYSPGPVLRGEVFDGADA 480

Query: 481 VRNMENWLIEIHGFQPQHSVTEMSEKEFWKMFDSELYEACRKKYGAIGTFMHVYYKCKKG 540
           VR ME+WLIE  GFQPQ++V+E+SEK+FW+MFD +LYE CRKKYGA+GTFM VYYK KKG
Sbjct: 481 VRRMEDWLIENRGFQPQYAVSELSEKKFWRMFDGDLYEHCRKKYGAVGTFMSVYYKSKKG 540

Query: 541 RKTEKEVEEAEKAQAETAYAD 562
           RKTEKEV+EAE+A  ETAYA+
Sbjct: 541 RKTEKEVQEAEQAHLETAYAE 561

BLAST of Csa4G103350 vs. TrEMBL
Match: F6HGH6_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_01s0010g01200 PE=4 SV=1)

HSP 1 Score: 988.8 bits (2555), Expect = 2.7e-285
Identity = 458/563 (81.35%), Postives = 514/563 (91.30%), Query Frame = 1

Query: 1   MTDLEASKPQKRKKGWVDILVNFRWIIVIFVVLPFSFSFYFIQYLGDKRSERKSYKQRQI 60
           M+DL+A    KRKK WVD  V+FRWIIVIFVVLP SF+ YF+ YLGD RSE KS+KQRQ 
Sbjct: 54  MSDLQAPLRPKRKKIWVDYFVHFRWIIVIFVVLPISFTLYFLTYLGDVRSESKSFKQRQE 113

Query: 61  EHEENVQKVVERLKQRNPSKDGLVCTARKPWLAVGMRNVDYKRARHFEVDLSAFRNILDI 120
           EH ENV+KV++RLK+RNPS+DGLVCTARKPW+AVGMRNVDYKRARHFEVDLSAFRNILDI
Sbjct: 114 EHNENVKKVIKRLKERNPSRDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI 173

Query: 121 DKERMIAKVEPHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS 180
           DKERMIA+ EP VNMGQISR ++PMNL+LAVVAELDDLTVGGLINGYGIEGSSHIYGLFS
Sbjct: 174 DKERMIARCEPLVNMGQISRVSVPMNLALAVVAELDDLTVGGLINGYGIEGSSHIYGLFS 233

Query: 181 DTVVAYEIVLADGRVVRATKDNEYSDLFYAIPWSQGTLGFLVSAEIKLIHIKEYMKLTYL 240
           DTVVAYEI+LADGR+VRATKDNEYSDLFYAIPWSQGTLG LV+AEIKLI IKEYMKLTY 
Sbjct: 234 DTVVAYEIILADGRLVRATKDNEYSDLFYAIPWSQGTLGLLVAAEIKLIPIKEYMKLTYK 293

Query: 241 PVKGNLQEIGQAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYASKEEAKKK 300
           PV GNL+++ Q Y+DSF PRD DQDNPEKVPDFVETMIY+PTEAVCMTGRYASKEEAKKK
Sbjct: 294 PVVGNLKDLAQGYLDSFAPRDGDQDNPEKVPDFVETMIYNPTEAVCMTGRYASKEEAKKK 353

Query: 301 GNVINNIGWWFKPWFYQHAQKSLKNGEFVEYIPTREYYHRHTKSLYWEGKLILPFADQWW 360
           GNVIN++GWW+KPWFYQHAQ +LK GEFVEYIPTREYYHRHT+ LYWEGKLILPFADQWW
Sbjct: 354 GNVINSVGWWYKPWFYQHAQTALKKGEFVEYIPTREYYHRHTRCLYWEGKLILPFADQWW 413

Query: 361 FRFFLGWMMPPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWIHREMEVYPL 420
           FRF  GW+MPPKVSLLKATQGEAIRNYYHEMH++QDMLVPL ++GD LEW+H EMEVYP+
Sbjct: 414 FRFLFGWLMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVGDALEWVHHEMEVYPI 473

Query: 421 WLCPHRLFKPPMKTMVSPEPGFELQLKQGDTPYAQMYTDIGVYYAPGPVLRGEVYDGVQA 480
           WLCPHRL+K P+KTM+ PEPGFEL  +QGDT YAQMYTD+GVYYAPGPVLRGE +DG +A
Sbjct: 474 WLCPHRLYKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGVYYAPGPVLRGEQFDGAEA 533

Query: 481 VRNMENWLIEIHGFQPQHSVTEMSEKEFWKMFDSELYEACRKKYGAIGTFMHVYYKCKKG 540
           VR MENWLIE HGFQPQ++V+E++EK FW+MFD+ LYE CRKKYGA+GTFM VYYKCKKG
Sbjct: 534 VRRMENWLIENHGFQPQYAVSELTEKNFWRMFDAGLYEHCRKKYGAVGTFMSVYYKCKKG 593

Query: 541 RKTEKEVEEAEKAQAETAYADVE 564
           +KTEKEV+EAE+AQ ET YA+ +
Sbjct: 594 KKTEKEVQEAEQAQLETPYAEAD 616

BLAST of Csa4G103350 vs. TrEMBL
Match: A5BTG6_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_016141 PE=4 SV=1)

HSP 1 Score: 985.7 bits (2547), Expect = 2.3e-284
Identity = 457/563 (81.17%), Postives = 513/563 (91.12%), Query Frame = 1

Query: 1   MTDLEASKPQKRKKGWVDILVNFRWIIVIFVVLPFSFSFYFIQYLGDKRSERKSYKQRQI 60
           M+DL+A    KRKK WVD  V+FRWIIVIFVVLP SF+ YF+ YLGD RSE KS+KQRQ 
Sbjct: 1   MSDLQAPLRPKRKKIWVDYFVHFRWIIVIFVVLPISFTLYFLTYLGDVRSESKSFKQRQE 60

Query: 61  EHEENVQKVVERLKQRNPSKDGLVCTARKPWLAVGMRNVDYKRARHFEVDLSAFRNILDI 120
           EH ENV+KV++RLK+RNPS+DGLVCTARKPW+AVGMRNVDYKRARHFEVDLSAFRNILDI
Sbjct: 61  EHNENVKKVIKRLKERNPSRDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI 120

Query: 121 DKERMIAKVEPHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS 180
           DKERMIA+ EP VNMGQISR ++PMNL+LAVVAELDDLTVGGLINGYGIEGSSHIYGLFS
Sbjct: 121 DKERMIARCEPLVNMGQISRVSVPMNLALAVVAELDDLTVGGLINGYGIEGSSHIYGLFS 180

Query: 181 DTVVAYEIVLADGRVVRATKDNEYSDLFYAIPWSQGTLGFLVSAEIKLIHIKEYMKLTYL 240
           DTVVAYEI+LADGR+VRATKDNEYSDLFYAIPWSQGTLG LV+AEIKLI IKEYMKLTY 
Sbjct: 181 DTVVAYEIILADGRLVRATKDNEYSDLFYAIPWSQGTLGLLVAAEIKLIPIKEYMKLTYK 240

Query: 241 PVKGNLQEIGQAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYASKEEAKKK 300
           PV GNL+++ Q Y+DSF PRD DQDN EKVPDFVETMIY+PTEAVCMTGRYASKEEAKKK
Sbjct: 241 PVVGNLKDLAQGYLDSFAPRDGDQDNXEKVPDFVETMIYNPTEAVCMTGRYASKEEAKKK 300

Query: 301 GNVINNIGWWFKPWFYQHAQKSLKNGEFVEYIPTREYYHRHTKSLYWEGKLILPFADQWW 360
           GNVIN++GWW+KPWFYQHAQ +LK GEFVEYIPTREYYHRHT+ LYWEGKLILPFADQWW
Sbjct: 301 GNVINSVGWWYKPWFYQHAQTALKKGEFVEYIPTREYYHRHTRCLYWEGKLILPFADQWW 360

Query: 361 FRFFLGWMMPPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWIHREMEVYPL 420
           FRF  GW+MPPKVSLLKATQGEAIRNYYHEMH++QDMLVPL ++GD LEW+H EMEVYP+
Sbjct: 361 FRFLFGWLMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVGDALEWVHHEMEVYPI 420

Query: 421 WLCPHRLFKPPMKTMVSPEPGFELQLKQGDTPYAQMYTDIGVYYAPGPVLRGEVYDGVQA 480
           WLCPHRL+K P+KTM+ PEPGFEL  +QGDT YAQMYTD+GVYYAPGPVLRGE +DG +A
Sbjct: 421 WLCPHRLYKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGVYYAPGPVLRGEQFDGAEA 480

Query: 481 VRNMENWLIEIHGFQPQHSVTEMSEKEFWKMFDSELYEACRKKYGAIGTFMHVYYKCKKG 540
           VR MENWLIE HGFQPQ++V+E++EK FW+MFD+ LYE CRKKYGA+GTFM VYYKCKKG
Sbjct: 481 VRRMENWLIENHGFQPQYAVSELTEKNFWRMFDAGLYEHCRKKYGAVGTFMSVYYKCKKG 540

Query: 541 RKTEKEVEEAEKAQAETAYADVE 564
           +KTEKEV+EAE+AQ ET YA+ +
Sbjct: 541 KKTEKEVQEAEQAQLETPYAEAD 563

BLAST of Csa4G103350 vs. TrEMBL
Match: A0A169WV31_DAUCA (Uncharacterized protein OS=Daucus carota subsp. sativus GN=DCAR_002565 PE=4 SV=1)

HSP 1 Score: 980.7 bits (2534), Expect = 7.5e-283
Identity = 459/563 (81.53%), Postives = 516/563 (91.65%), Query Frame = 1

Query: 1   MTDLEASKPQKRKKGWVDILVNFRWIIVIFVVLPFSFSFYFIQYLGDKRSERKSYKQRQI 60
           M+DLEA    KRKK WVD  VNFRWIIVIFVVLP SF+ YF+ YLGD RSE KS+++RQ 
Sbjct: 1   MSDLEAPLRPKRKKIWVDYFVNFRWIIVIFVVLPISFTLYFLTYLGDVRSEWKSFEKRQK 60

Query: 61  EHEENVQKVVERLKQRNPSKDGLVCTARKPWLAVGMRNVDYKRARHFEVDLSAFRNILDI 120
           EH+ENV+KVV+RLKQRNPSKDGLVCTARKP++AVGMRNVDYKRARHFEVDLSAFRNIL+I
Sbjct: 61  EHDENVKKVVKRLKQRNPSKDGLVCTARKPYIAVGMRNVDYKRARHFEVDLSAFRNILEI 120

Query: 121 DKERMIAKVEPHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS 180
           D+ERMIAK EP VNMGQI+R T+PMNL+LAVVAELDDLTVGGLINGYGIEGSSH YGLF+
Sbjct: 121 DQERMIAKCEPLVNMGQITRVTVPMNLALAVVAELDDLTVGGLINGYGIEGSSHKYGLFA 180

Query: 181 DTVVAYEIVLADGRVVRATKDNEYSDLFYAIPWSQGTLGFLVSAEIKLIHIKEYMKLTYL 240
           DTVVAYEIVLADG+VVRATKDNEYSDLFYAIPWSQGTLG LVSAEIKLI IKEYMKLTY 
Sbjct: 181 DTVVAYEIVLADGKVVRATKDNEYSDLFYAIPWSQGTLGLLVSAEIKLIPIKEYMKLTYT 240

Query: 241 PVKGNLQEIGQAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYASKEEAKKK 300
           PV+GNL+E+ Q Y+DSF PRD DQDN EKVPDFVETMIY+P+EAVCMTGRYASKEEAKKK
Sbjct: 241 PVRGNLKELAQGYIDSFAPRDGDQDNDEKVPDFVETMIYNPSEAVCMTGRYASKEEAKKK 300

Query: 301 GNVINNIGWWFKPWFYQHAQKSLKNGEFVEYIPTREYYHRHTKSLYWEGKLILPFADQWW 360
           GN IN +GWWFKPWFYQHA+K+LK GEFVEYIPTREYYHRHT+ LYWEGKLILPFADQWW
Sbjct: 301 GNKINEVGWWFKPWFYQHAEKALKKGEFVEYIPTREYYHRHTRCLYWEGKLILPFADQWW 360

Query: 361 FRFFLGWMMPPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWIHREMEVYPL 420
           FR+ LGW+MPPKVSLLKATQGEAIRNYYHEMH++QDMLVPL ++GD LE++HREME+YPL
Sbjct: 361 FRYTLGWLMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVGDALEFVHREMELYPL 420

Query: 421 WLCPHRLFKPPMKTMVSPEPGFELQLKQGDTPYAQMYTDIGVYYAPGPVLRGEVYDGVQA 480
           WLCPH+L+K P KTM+ PEPGFEL  +QGDT YAQMYTD+GVYYAPGPVLRGE +DG +A
Sbjct: 421 WLCPHKLYKLPCKTMIYPEPGFELHHRQGDTHYAQMYTDVGVYYAPGPVLRGEEFDGAEA 480

Query: 481 VRNMENWLIEIHGFQPQHSVTEMSEKEFWKMFDSELYEACRKKYGAIGTFMHVYYKCKKG 540
           VR +E+WLIE HGFQPQ++V+E+SEK FWKMFD+ELYE CR+KYGA+GTFM VYYKCKKG
Sbjct: 481 VRRLESWLIENHGFQPQYAVSELSEKNFWKMFDAELYEQCRRKYGAVGTFMSVYYKCKKG 540

Query: 541 RKTEKEVEEAEKAQAETAYADVE 564
           RKTEKEV+EAE+A  ETAYA+V+
Sbjct: 541 RKTEKEVQEAEQAHLETAYAEVD 563

BLAST of Csa4G103350 vs. TAIR10
Match: AT3G19820.1 (AT3G19820.1 cell elongation protein / DWARF1 / DIMINUTO (DIM))

HSP 1 Score: 903.3 bits (2333), Expect = 7.7e-263
Identity = 423/564 (75.00%), Postives = 489/564 (86.70%), Query Frame = 1

Query: 1   MTDLEASKPQ-KRKKGWVDILVNFRWIIVIFVVLPFSFSFYFIQYLGDKRSERKSYKQRQ 60
           M+DL+    + KRKK WVD  V FRWIIVIF+VLPFS +FYF+ YLGD  SE KS+++RQ
Sbjct: 1   MSDLQTPLVRPKRKKTWVDYFVKFRWIIVIFIVLPFSATFYFLIYLGDMWSESKSFEKRQ 60

Query: 61  IEHEENVQKVVERLKQRNPSKDGLVCTARKPWLAVGMRNVDYKRARHFEVDLSAFRNILD 120
            EH+ENV+KV++RLK R+ SKDGLVCTARKPW+AVGMRNVDYKRARHFEVDL  FRNIL+
Sbjct: 61  KEHDENVKKVIKRLKGRDASKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLGEFRNILE 120

Query: 121 IDKERMIAKVEPHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLF 180
           I+KE+M A+VEP VNMGQISRAT+PMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLF
Sbjct: 121 INKEKMTARVEPLVNMGQISRATVPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLF 180

Query: 181 SDTVVAYEIVLADGRVVRATKDNEYSDLFYAIPWSQGTLGFLVSAEIKLIHIKEYMKLTY 240
           +DTV AYEIVLA G +VRAT+DNEYSDL+YAIPWSQGTLG LV+AEI+LI +KEYM+LTY
Sbjct: 181 ADTVEAYEIVLAGGELVRATRDNEYSDLYYAIPWSQGTLGLLVAAEIRLIKVKEYMRLTY 240

Query: 241 LPVKGNLQEIGQAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYASKEEAKK 300
           +PVKG+LQ + Q Y+DSF P+D D+    K+PDFVE M+Y+PTE V M G YASKEEAKK
Sbjct: 241 IPVKGDLQALAQGYIDSFAPKDGDKS---KIPDFVEGMVYNPTEGVMMVGTYASKEEAKK 300

Query: 301 KGNVINNIGWWFKPWFYQHAQKSLKNGEFVEYIPTREYYHRHTKSLYWEGKLILPFADQW 360
           KGN INN+GWWFKPWFYQHAQ +LK G+FVEYIPTREYYHRHT+ LYWEGKLILPF DQ+
Sbjct: 301 KGNKINNVGWWFKPWFYQHAQTALKKGQFVEYIPTREYYHRHTRCLYWEGKLILPFGDQF 360

Query: 361 WFRFFLGWMMPPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWIHREMEVYP 420
           WFR+ LGW+MPPKVSLLKATQGEAIRNYYH+MH++QDMLVPL ++GD LEW+HREMEVYP
Sbjct: 361 WFRYLLGWLMPPKVSLLKATQGEAIRNYYHDMHVIQDMLVPLYKVGDALEWVHREMEVYP 420

Query: 421 LWLCPHRLFKPPMKTMVSPEPGFELQLKQGDTPYAQMYTDIGVYYAPGPVLRGEVYDGVQ 480
           +WLCPH+LFK P+K  + PEPGFE + +QGDT  AQMYTD+GVYYAPG VLRGE +DG +
Sbjct: 421 IWLCPHKLFKQPIKGQIYPEPGFEYENRQGDTEDAQMYTDVGVYYAPGCVLRGEEFDGSE 480

Query: 481 AVRNMENWLIEIHGFQPQHSVTEMSEKEFWKMFDSELYEACRKKYGAIGTFMHVYYKCKK 540
           AVR ME WLIE HGFQPQ++V+E+ EK FW+MF+ ELYE CRKKY AIGTFM VYYK KK
Sbjct: 481 AVRRMEKWLIENHGFQPQYAVSELDEKSFWRMFNGELYEECRKKYRAIGTFMSVYYKSKK 540

Query: 541 GRKTEKEVEEAEKAQAETAYADVE 564
           GRKTEKEV EAE+A  ETAYA+ +
Sbjct: 541 GRKTEKEVREAEQAHLETAYAEAD 561

BLAST of Csa4G103350 vs. NCBI nr
Match: gi|449438803|ref|XP_004137177.1| (PREDICTED: delta(24)-sterol reductase-like [Cucumis sativus])

HSP 1 Score: 1175.6 bits (3040), Expect = 0.0e+00
Identity = 567/567 (100.00%), Postives = 567/567 (100.00%), Query Frame = 1

Query: 1   MTDLEASKPQKRKKGWVDILVNFRWIIVIFVVLPFSFSFYFIQYLGDKRSERKSYKQRQI 60
           MTDLEASKPQKRKKGWVDILVNFRWIIVIFVVLPFSFSFYFIQYLGDKRSERKSYKQRQI
Sbjct: 1   MTDLEASKPQKRKKGWVDILVNFRWIIVIFVVLPFSFSFYFIQYLGDKRSERKSYKQRQI 60

Query: 61  EHEENVQKVVERLKQRNPSKDGLVCTARKPWLAVGMRNVDYKRARHFEVDLSAFRNILDI 120
           EHEENVQKVVERLKQRNPSKDGLVCTARKPWLAVGMRNVDYKRARHFEVDLSAFRNILDI
Sbjct: 61  EHEENVQKVVERLKQRNPSKDGLVCTARKPWLAVGMRNVDYKRARHFEVDLSAFRNILDI 120

Query: 121 DKERMIAKVEPHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS 180
           DKERMIAKVEPHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS
Sbjct: 121 DKERMIAKVEPHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS 180

Query: 181 DTVVAYEIVLADGRVVRATKDNEYSDLFYAIPWSQGTLGFLVSAEIKLIHIKEYMKLTYL 240
           DTVVAYEIVLADGRVVRATKDNEYSDLFYAIPWSQGTLGFLVSAEIKLIHIKEYMKLTYL
Sbjct: 181 DTVVAYEIVLADGRVVRATKDNEYSDLFYAIPWSQGTLGFLVSAEIKLIHIKEYMKLTYL 240

Query: 241 PVKGNLQEIGQAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYASKEEAKKK 300
           PVKGNLQEIGQAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYASKEEAKKK
Sbjct: 241 PVKGNLQEIGQAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYASKEEAKKK 300

Query: 301 GNVINNIGWWFKPWFYQHAQKSLKNGEFVEYIPTREYYHRHTKSLYWEGKLILPFADQWW 360
           GNVINNIGWWFKPWFYQHAQKSLKNGEFVEYIPTREYYHRHTKSLYWEGKLILPFADQWW
Sbjct: 301 GNVINNIGWWFKPWFYQHAQKSLKNGEFVEYIPTREYYHRHTKSLYWEGKLILPFADQWW 360

Query: 361 FRFFLGWMMPPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWIHREMEVYPL 420
           FRFFLGWMMPPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWIHREMEVYPL
Sbjct: 361 FRFFLGWMMPPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWIHREMEVYPL 420

Query: 421 WLCPHRLFKPPMKTMVSPEPGFELQLKQGDTPYAQMYTDIGVYYAPGPVLRGEVYDGVQA 480
           WLCPHRLFKPPMKTMVSPEPGFELQLKQGDTPYAQMYTDIGVYYAPGPVLRGEVYDGVQA
Sbjct: 421 WLCPHRLFKPPMKTMVSPEPGFELQLKQGDTPYAQMYTDIGVYYAPGPVLRGEVYDGVQA 480

Query: 481 VRNMENWLIEIHGFQPQHSVTEMSEKEFWKMFDSELYEACRKKYGAIGTFMHVYYKCKKG 540
           VRNMENWLIEIHGFQPQHSVTEMSEKEFWKMFDSELYEACRKKYGAIGTFMHVYYKCKKG
Sbjct: 481 VRNMENWLIEIHGFQPQHSVTEMSEKEFWKMFDSELYEACRKKYGAIGTFMHVYYKCKKG 540

Query: 541 RKTEKEVEEAEKAQAETAYADVEQAGE 568
           RKTEKEVEEAEKAQAETAYADVEQAGE
Sbjct: 541 RKTEKEVEEAEKAQAETAYADVEQAGE 567

BLAST of Csa4G103350 vs. NCBI nr
Match: gi|659120557|ref|XP_008460246.1| (PREDICTED: delta(24)-sterol reductase-like [Cucumis melo])

HSP 1 Score: 1147.5 bits (2967), Expect = 0.0e+00
Identity = 552/565 (97.70%), Postives = 557/565 (98.58%), Query Frame = 1

Query: 3   DLEASKPQKRKKGWVDILVNFRWIIVIFVVLPFSFSFYFIQYLGDKRSERKSYKQRQIEH 62
           DLEASKPQKRKKGWVDILVNFRWIIVIFVVLPFSFSFYFIQYLGDKRSERKSYKQRQIEH
Sbjct: 2   DLEASKPQKRKKGWVDILVNFRWIIVIFVVLPFSFSFYFIQYLGDKRSERKSYKQRQIEH 61

Query: 63  EENVQKVVERLKQRNPSKDGLVCTARKPWLAVGMRNVDYKRARHFEVDLSAFRNILDIDK 122
           EENVQKVVERLKQRNPSKDGLVCTARKPWLAVGMRNVDYKRARHFEVDLSAFRNILDIDK
Sbjct: 62  EENVQKVVERLKQRNPSKDGLVCTARKPWLAVGMRNVDYKRARHFEVDLSAFRNILDIDK 121

Query: 123 ERMIAKVEPHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFSDT 182
           ERMIAKVEPHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFSDT
Sbjct: 122 ERMIAKVEPHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFSDT 181

Query: 183 VVAYEIVLADGRVVRATKDNEYSDLFYAIPWSQGTLGFLVSAEIKLIHIKEYMKLTYLPV 242
           VVAYEIVLADGRVVRATKDNEYSDLFYAIPWSQGTLGFLVSAEIKLIHIKEYMKLTYLPV
Sbjct: 182 VVAYEIVLADGRVVRATKDNEYSDLFYAIPWSQGTLGFLVSAEIKLIHIKEYMKLTYLPV 241

Query: 243 KGNLQEIGQAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYASKEEAKKKGN 302
           KG LQEIGQAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYASKEEAKKKGN
Sbjct: 242 KGKLQEIGQAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYASKEEAKKKGN 301

Query: 303 VINNIGWWFKPWFYQHAQKSLKNGEFVEYIPTREYYHRHTKSLYWEGKLILPFADQWWFR 362
           VIN+IGWWFKPWFYQHAQK+L  GEFVEYIPTREYYHRHT+SLYWEGKLILPFADQWWFR
Sbjct: 302 VINSIGWWFKPWFYQHAQKALIKGEFVEYIPTREYYHRHTRSLYWEGKLILPFADQWWFR 361

Query: 363 FFLGWMMPPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWIHREMEVYPLWL 422
           F LGWMMPPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWIHREMEVYPLWL
Sbjct: 362 FLLGWMMPPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWIHREMEVYPLWL 421

Query: 423 CPHRLFKPPMKTMVSPEPGFELQLKQGDTPYAQMYTDIGVYYAPGPVLRGEVYDGVQAVR 482
           CPHRLFKPPMKTMVSPEPGFELQ+KQGDT YAQMYTDIGVYYAPGPVLRGEVYDG QAVR
Sbjct: 422 CPHRLFKPPMKTMVSPEPGFELQIKQGDTSYAQMYTDIGVYYAPGPVLRGEVYDGAQAVR 481

Query: 483 NMENWLIEIHGFQPQHSVTEMSEKEFWKMFDSELYEACRKKYGAIGTFMHVYYKCKKGRK 542
           NMENWLIEIHGFQPQHSVTEMSEKEFWKMFDSELYEACRKKYGAIGTFMHVYYKCKKGRK
Sbjct: 482 NMENWLIEIHGFQPQHSVTEMSEKEFWKMFDSELYEACRKKYGAIGTFMHVYYKCKKGRK 541

Query: 543 TEKEVEEAEKAQAETAYADVEQAGE 568
           TEKE+EEAE AQAETAYADVEQA E
Sbjct: 542 TEKEIEEAENAQAETAYADVEQASE 566

BLAST of Csa4G103350 vs. NCBI nr
Match: gi|703159643|ref|XP_010112301.1| (Delta(24)-sterol reductase [Morus notabilis])

HSP 1 Score: 999.6 bits (2583), Expect = 2.2e-288
Identity = 466/567 (82.19%), Postives = 520/567 (91.71%), Query Frame = 1

Query: 1   MTDLEASKPQKRKKGWVDILVNFRWIIVIFVVLPFSFSFYFIQYLGDKRSERKSYKQRQI 60
           M+DLEA    KRKK WVD  V FRWI+VIFVVLP SF+ YF+ YLGD +SERKS+KQRQ 
Sbjct: 1   MSDLEAPLRPKRKKIWVDYFVQFRWILVIFVVLPISFTLYFLTYLGDVKSERKSFKQRQK 60

Query: 61  EHEENVQKVVERLKQRNPSKDGLVCTARKPWLAVGMRNVDYKRARHFEVDLSAFRNILDI 120
           EH+ENVQKV++RLKQR+PSKDGLVCTARKPW+AVGMRNVDYKRARHFEVDLSAFRNILDI
Sbjct: 61  EHDENVQKVIKRLKQRDPSKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI 120

Query: 121 DKERMIAKVEPHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS 180
           DK+RMIA+VEP VNMGQISRAT+PMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS
Sbjct: 121 DKDRMIARVEPLVNMGQISRATVPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS 180

Query: 181 DTVVAYEIVLADGRVVRATKDNEYSDLFYAIPWSQGTLGFLVSAEIKLIHIKEYMKLTYL 240
           DTVVAYEIVLADGRVVRATKDNE+SDLFYAIPWSQGTLG LV+AEIKLI +KEYM++TY 
Sbjct: 181 DTVVAYEIVLADGRVVRATKDNEFSDLFYAIPWSQGTLGLLVAAEIKLIPVKEYMRVTYK 240

Query: 241 PVKGNLQEIGQAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYASKEEAKKK 300
           PV GNLQE+GQ Y+DSF PRD DQDNP+KVPDFVETMIY+P EAVCMTGRYASKEEAKKK
Sbjct: 241 PVVGNLQELGQGYIDSFAPRDCDQDNPDKVPDFVETMIYNPAEAVCMTGRYASKEEAKKK 300

Query: 301 GNVINNIGWWFKPWFYQHAQKSLKNGEFVEYIPTREYYHRHTKSLYWEGKLILPFADQWW 360
           GNVIN++GWWFKPWFYQHAQ +LK GEFVEYIPTREYYHRHT+ LYWEGKLILPFADQWW
Sbjct: 301 GNVINSVGWWFKPWFYQHAQTALKKGEFVEYIPTREYYHRHTRCLYWEGKLILPFADQWW 360

Query: 361 FRFFLGWMMPPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWIHREMEVYPL 420
           FRF LGWMMPPKVSLLKATQGEAIRNYYHEMH++QDMLVPL ++GD LEW+H+EMEVYP+
Sbjct: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVGDALEWVHQEMEVYPI 420

Query: 421 WLCPHRLFKPPMKTMVSPEPGFELQLKQGDTPYAQMYTDIGVYYAPGPVLRGEVYDGVQA 480
           WLCPHRL+K P+KTM+ PEPGFEL  +QGDT YAQMYTD+GVYYAPGPVLRGEV+DG  A
Sbjct: 421 WLCPHRLYKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGVYYAPGPVLRGEVFDGAGA 480

Query: 481 VRNMENWLIEIHGFQPQHSVTEMSEKEFWKMFDSELYEACRKKYGAIGTFMHVYYKCKKG 540
           VR ME+WLI+ HGFQPQ++V+E+SEK FW+MFD+ LYE CR+KYGA+GTFM VYYK KKG
Sbjct: 481 VRKMEDWLIKNHGFQPQYAVSELSEKSFWRMFDAGLYEHCRRKYGAVGTFMSVYYKSKKG 540

Query: 541 RKTEKEVEEAEKAQAETAYADVEQAGE 568
           RKTEKEV EAE+A  ETAYA+V+Q  +
Sbjct: 541 RKTEKEVREAEQAHLETAYAEVDQPAD 567

BLAST of Csa4G103350 vs. NCBI nr
Match: gi|224098551|ref|XP_002311215.1| (diminuto family protein [Populus trichocarpa])

HSP 1 Score: 992.3 bits (2564), Expect = 3.6e-286
Identity = 469/561 (83.60%), Postives = 514/561 (91.62%), Query Frame = 1

Query: 1   MTDLEASKPQKRKKGWVDILVNFRWIIVIFVVLPFSFSFYFIQYLGDKRSERKSYKQRQI 60
           M+DLEA    KRKK WVD  V FRWI+VIFVVLP SF+ YF+ YLGD +SE KSYKQRQ 
Sbjct: 1   MSDLEAPLRPKRKKVWVDYFVQFRWILVIFVVLPISFTLYFLTYLGDVKSEMKSYKQRQK 60

Query: 61  EHEENVQKVVERLKQRNPSKDGLVCTARKPWLAVGMRNVDYKRARHFEVDLSAFRNILDI 120
           EH+ENV+KVV+RLK+RNPSKDGLVCTARKPW+AVGMRNVDYKRARHFEVDLSAFRNIL+I
Sbjct: 61  EHDENVKKVVKRLKERNPSKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILEI 120

Query: 121 DKERMIAKVEPHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS 180
           DKERMIA+VEP VNMGQISRA++PMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS
Sbjct: 121 DKERMIARVEPLVNMGQISRASVPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS 180

Query: 181 DTVVAYEIVLADGRVVRATKDNEYSDLFYAIPWSQGTLGFLVSAEIKLIHIKEYMKLTYL 240
           DTVVAYEIVLADG+VVRATKDNEYSDLFYAIPWSQGTLG LVSAEIKLI IKEYM+LTY 
Sbjct: 181 DTVVAYEIVLADGQVVRATKDNEYSDLFYAIPWSQGTLGLLVSAEIKLIPIKEYMRLTYK 240

Query: 241 PVKGNLQEIGQAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYASKEEAKKK 300
           PV GNL+E+ QAY+DSF PRD DQDNPEKVPDFVETMIY+ TE V MTGRYASKEEAKKK
Sbjct: 241 PVVGNLKELAQAYIDSFAPRDGDQDNPEKVPDFVETMIYNSTEGVMMTGRYASKEEAKKK 300

Query: 301 GNVINNIGWWFKPWFYQHAQKSLKNGEFVEYIPTREYYHRHTKSLYWEGKLILPFADQWW 360
           GNVINN+GWWFKPWFYQHAQ +LK GEFVEYIPTREYYHRHT+ LYWEGKLILPFADQWW
Sbjct: 301 GNVINNVGWWFKPWFYQHAQTALKKGEFVEYIPTREYYHRHTRCLYWEGKLILPFADQWW 360

Query: 361 FRFFLGWMMPPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWIHREMEVYPL 420
           FRF LGWMMPPKVSLLKATQGEAIRNYYHEMH++QDMLVPL ++GD LEW+H+EMEVYPL
Sbjct: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVGDALEWVHQEMEVYPL 420

Query: 421 WLCPHRLFKPPMKTMVSPEPGFELQLKQGDTPYAQMYTDIGVYYAPGPVLRGEVYDGVQA 480
           WLCPHRLFK P+KTMV PEPGFE Q KQGDT YAQMYTD+GVYY+PGPVLRGEV+DG  A
Sbjct: 421 WLCPHRLFKLPVKTMVYPEPGFEHQHKQGDTSYAQMYTDVGVYYSPGPVLRGEVFDGADA 480

Query: 481 VRNMENWLIEIHGFQPQHSVTEMSEKEFWKMFDSELYEACRKKYGAIGTFMHVYYKCKKG 540
           VR ME+WLIE  GFQPQ++V+E+SEK+FW+MFD +LYE CRKKYGA+GTFM VYYK KKG
Sbjct: 481 VRRMEDWLIENRGFQPQYAVSELSEKKFWRMFDGDLYEHCRKKYGAVGTFMSVYYKSKKG 540

Query: 541 RKTEKEVEEAEKAQAETAYAD 562
           RKTEKEV+EAE+A  ETAYA+
Sbjct: 541 RKTEKEVQEAEQAHLETAYAE 561

BLAST of Csa4G103350 vs. NCBI nr
Match: gi|743782362|ref|XP_011016908.1| (PREDICTED: delta(24)-sterol reductase-like [Populus euphratica])

HSP 1 Score: 991.9 bits (2563), Expect = 4.7e-286
Identity = 468/561 (83.42%), Postives = 515/561 (91.80%), Query Frame = 1

Query: 1   MTDLEASKPQKRKKGWVDILVNFRWIIVIFVVLPFSFSFYFIQYLGDKRSERKSYKQRQI 60
           M+DLEA    KRKK WVD  V FRWI+VIFVVLP SFS YF+ YLGD +SE KSYKQRQ 
Sbjct: 1   MSDLEAPLRPKRKKVWVDYFVQFRWILVIFVVLPISFSLYFLTYLGDVKSEMKSYKQRQK 60

Query: 61  EHEENVQKVVERLKQRNPSKDGLVCTARKPWLAVGMRNVDYKRARHFEVDLSAFRNILDI 120
           EH+ENV+KVV+RLK+RNPSKDGLVCTARKPW+AVGMRNVDYKRARHFEVDLSAFRNIL+I
Sbjct: 61  EHDENVKKVVKRLKERNPSKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILEI 120

Query: 121 DKERMIAKVEPHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS 180
           DKERMIA+VEP VNMGQISRA++PMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS
Sbjct: 121 DKERMIARVEPLVNMGQISRASVPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS 180

Query: 181 DTVVAYEIVLADGRVVRATKDNEYSDLFYAIPWSQGTLGFLVSAEIKLIHIKEYMKLTYL 240
           DTVVAYEIVLA+G+VVRATKDNEYSDLFYAIPWSQGTLG LVSAEIKLI +KEYM+LTY 
Sbjct: 181 DTVVAYEIVLANGQVVRATKDNEYSDLFYAIPWSQGTLGLLVSAEIKLIPVKEYMRLTYK 240

Query: 241 PVKGNLQEIGQAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYASKEEAKKK 300
           PV GNL+E+ QAY+DSF PRD DQDNPEKVPDFVETMIY+ TE V MTGRYASKEEAKKK
Sbjct: 241 PVVGNLKELAQAYIDSFAPRDGDQDNPEKVPDFVETMIYNSTEGVMMTGRYASKEEAKKK 300

Query: 301 GNVINNIGWWFKPWFYQHAQKSLKNGEFVEYIPTREYYHRHTKSLYWEGKLILPFADQWW 360
           GNVINN+GWWFKPWFYQHAQ +LK GEFVEYIPTREYYHRHT+ LYWEGKLILPFADQWW
Sbjct: 301 GNVINNVGWWFKPWFYQHAQTALKKGEFVEYIPTREYYHRHTRCLYWEGKLILPFADQWW 360

Query: 361 FRFFLGWMMPPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWIHREMEVYPL 420
           FRF LGWMMPPKVSLLKATQGEAIRNYYHEMH++QDMLVPL ++GD LEW+H+EMEVYPL
Sbjct: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVGDALEWVHQEMEVYPL 420

Query: 421 WLCPHRLFKPPMKTMVSPEPGFELQLKQGDTPYAQMYTDIGVYYAPGPVLRGEVYDGVQA 480
           WLCPHRLFK P+KTMV PEPGFE Q +QGDT YAQMYTD+GVYYAPGPVLRGEV+DG  A
Sbjct: 421 WLCPHRLFKLPVKTMVYPEPGFEHQQRQGDTSYAQMYTDVGVYYAPGPVLRGEVFDGADA 480

Query: 481 VRNMENWLIEIHGFQPQHSVTEMSEKEFWKMFDSELYEACRKKYGAIGTFMHVYYKCKKG 540
           VR ME+WLIE  GFQPQ++V+E+SEK+FW+MFD++LYE CRKKYGA+GTFM VYYK KKG
Sbjct: 481 VRRMEDWLIENRGFQPQYAVSELSEKKFWRMFDADLYEHCRKKYGAVGTFMSVYYKSKKG 540

Query: 541 RKTEKEVEEAEKAQAETAYAD 562
           RKTEKEV+EAE+A  ETAYA+
Sbjct: 541 RKTEKEVQEAEQAHLETAYAE 561