Csa4G016360 (gene) Cucumber (Chinese Long) v2

BLAST of Csa4G016360 vs. Swiss-Prot
Match: RGA3_SOLBU (Putative disease resistance protein RGA3 OS=Solanum bulbocastanum GN=RGA3 PE=2 SV=2)

HSP 1 Score: 308.1 bits (788), Expect = 3.3e-82
Identity = 251/764 (32.85%), Postives = 378/764 (49.48%), Query Frame = 1

Query: 201 WEELEDCLKEIVGKFGITIIVTTRLDEVANIMGTVSGYRLEKLPEDHCWSLFKRSANANG 260
           W+ L   LK  +G  G +I++TTRL+++ +IMGT+  Y+L  L ++ CW LFK+ A  + 
Sbjct: 271 WDNLRAVLK--IGASGASILITTRLEKIGSIMGTLQLYQLSNLSQEDCWLLFKQRAFCHQ 330

Query: 261 VKMTPKLEAIRIKLLQKIDGIPLVAKVLGGAVEFEGDLDRWETTLESIVREIPMKQKSYV 320
            + +PKL  I  ++++K  G+PL AK LGG + F+ +   WE   +S +  +P  + S V
Sbjct: 331 TETSPKLMEIGKEIVKKCGGVPLAAKTLGGLLRFKREESEWEHVRDSEIWNLPQDENS-V 390

Query: 321 LSILQLSVDRLPFVEKQCFAYCSIFPKDCEVVKENLIRMWIAQGFIQPTEGENTMEDLGE 380
           L  L+LS   LP   +QCFAYC++FPKD ++ KE LI +W+A  F+  ++G   +ED+G 
Sbjct: 391 LPALRLSYHHLPLDLRQCFAYCAVFPKDTKIEKEYLIALWMAHSFLL-SKGNMELEDVGN 450

Query: 381 GHFNFLLSRSLFQDVVKDKYGRITHFKMHDLIHDVALAILST-------RQKSVLDPTHW 440
             +N L  RS FQ+ ++ K G+ T+FKMHDLIHD+A ++ S        RQ +V D    
Sbjct: 451 EVWNELYLRSFFQE-IEVKSGK-TYFKMHDLIHDLATSMFSASASSRSIRQINVKDDEDM 510

Query: 441 NGKTSRKLRTLLYNNQEIHHKVADCVFLRVLEVNSLHMMNN----LPDFIAKLKHLRYLD 500
               +     +     E+    +  +F R + +  L++ N+    LP  +  L HLRYLD
Sbjct: 511 MFIVTNYKDMMSIGFSEVVSSYSPSLFKRFVSLRVLNLSNSEFEQLPSSVGDLVHLRYLD 570

Query: 501 ISSCSMWVMPHSVTTLFNLQTLKL---GSIENLPMNLRNLVRLRHLEFHVYYNTRKMPSH 560
           +S   +  +P  +  L NLQTL L    S+  LP     L  LR+L    +     MP  
Sbjct: 571 LSGNKICSLPKRLCKLQNLQTLDLYNCQSLSCLPKQTSKLCSLRNLVLD-HCPLTSMPPR 630

Query: 561 MGELIHLQILSWFVAGFEEGCKIEELGNLKNLKGQLQLSNLEQVRSKEEALAAKLVNKKN 620
           +G L  L+ L +FV G  +G ++ EL NL NL+G + +++LE+V++  EA  A L  K N
Sbjct: 631 IGLLTCLKTLGYFVVGERKGYQLGELRNL-NLRGAISITHLERVKNDMEAKEANLSAKAN 690

Query: 621 LRELTFEWSIDILRECSSYNDFEVLEGLQPPKNLSSLKITNFGGKFLP---AATFVENLV 680
           L  L+  W      E     + +VLE L+P  NL  L+I +F G  LP     + ++N+V
Sbjct: 691 LHSLSMSWDRPNRYES---EEVKVLEALKPHPNLKYLEIIDFCGFCLPDWMNHSVLKNVV 750

Query: 681 FLCLYGCTKCERLPMLGQLANLQELSICFMDSVRSIGSEFYGIDSNRRGYFPKLKKFDFC 740
            + + GC  C  LP  G+L  L+ L +    SV     E  G  + RR  FP L+K    
Sbjct: 751 SILISGCENCSCLPPFGELPCLESLEL-QDGSVEVEYVEDSGFLTRRR--FPSLRKLHIG 810

Query: 741 WMCNLEQWELEVANHESNHFGSLQTLKLDRCGKLTKLPNGLECCKSVHEVIISNCPNLTL 800
             CNL+                LQ +K            G E    + E+ IS+CP    
Sbjct: 811 GFCNLK---------------GLQRMK------------GAEQFPVLEEMKISDCPMFVF 870

Query: 801 NVEEMHNLSVLLIDGLKFLPKGLALHPNLKTIMIKGCIEDYDYSP-----FLNLPSLTKL 860
               + ++  L I G +    GL+   NL T+       ++  +      F NL +L  +
Sbjct: 871 --PTLSSVKKLEIWG-EADAGGLSSISNLSTLTSLKIFSNHTVTSLLEEMFKNLENL--I 930

Query: 861 YLN-DGLGNATQLPKQLQHLTALKILAIENFYGIEVLP-EWLRKLTCLETLDLVRCKNLK 920
           YL+   L N  +LP  L  L  LK L I   Y +E LP E L  L+ L  L +  C  LK
Sbjct: 931 YLSVSFLENLKELPTSLASLNNLKCLDIRYCYALESLPEEGLEGLSSLTELFVEHCNMLK 986

Query: 921 RLPSRGAMRCLTKLKDFKVIACPLLLLGGQ--ADQEGAKYLHIP 939
            LP    ++ LT L   K+  CP L+   +    ++  K  HIP
Sbjct: 991 CLPE--GLQHLTTLTSLKIRGCPQLIKRCEKGIGEDWHKISHIP 986

BLAST of Csa4G016360 vs. Swiss-Prot
Match: RGA2_SOLBU (Disease resistance protein RGA2 OS=Solanum bulbocastanum GN=RGA2 PE=1 SV=1)

HSP 1 Score: 296.6 bits (758), Expect = 9.9e-79
Identity = 246/759 (32.41%), Postives = 370/759 (48.75%), Query Frame = 1

Query: 201 WEELEDCLKEIVGKFGITIIVTTRLDEVANIMGTVSGYRLEKLPEDHCWSLFKRSANANG 260
           W  L   LK  VG  G +++ TTRL++V +IMGT+  Y L  L ++ CW LF + A  + 
Sbjct: 272 WANLRAVLK--VGASGASVLTTTRLEKVGSIMGTLQPYELSNLSQEDCWLLFMQRAFGHQ 331

Query: 261 VKMTPKLEAIRIKLLQKIDGIPLVAKVLGGAVEFEGDLDRWETTLESIVREIPMKQKSYV 320
            ++ P L AI  ++++K  G+PL AK LGG + F+ +   WE   +S +  +P  + S +
Sbjct: 332 EEINPNLVAIGKEIVKKSGGVPLAAKTLGGILCFKREERAWEHVRDSPIWNLPQDESS-I 391

Query: 321 LSILQLSVDRLPFVEKQCFAYCSIFPKDCEVVKENLIRMWIAQGFIQPTEGENTMEDLGE 380
           L  L+LS  +LP   KQCFAYC++FPKD ++ KE LI +W+A GF+  ++G   +ED+G+
Sbjct: 392 LPALRLSYHQLPLDLKQCFAYCAVFPKDAKMEKEKLISLWMAHGFLL-SKGNMELEDVGD 451

Query: 381 GHFNFLLSRSLFQDV-VKDKYGRITHFKMHDLIHDVALAILSTRQKSVLDPTHWNGKTSR 440
             +  L  RS FQ++ VKD  G+ T+FKMHDLIHD+A ++ S    S  +    N  +  
Sbjct: 452 EVWKELYLRSFFQEIEVKD--GK-TYFKMHDLIHDLATSLFSANTSS-SNIREINKHSYT 511

Query: 441 KLRTLLYNNQEIHHKVADC---VFLRVLEVNSLHMMNNLPDFIAKLKHLRYLDISSCSMW 500
            + ++ +      + +      + LRVL +      N LP  I  L HLRYL++    M 
Sbjct: 512 HMMSIGFAEVVFFYTLPPLEKFISLRVLNLGD-STFNKLPSSIGDLVHLRYLNLYGSGMR 571

Query: 501 VMPHSVTTLFNLQTLKLGSIEN---LPMNLRNLVRLRHLEFHVYYNTRKMPSHMGELIHL 560
            +P  +  L NLQTL L        LP     L  LR+L      +   MP  +G L  L
Sbjct: 572 SLPKQLCKLQNLQTLDLQYCTKLCCLPKETSKLGSLRNLLLDGSQSLTCMPPRIGSLTCL 631

Query: 561 QILSWFVAGFEEGCKIEELGNLKNLKGQLQLSNLEQVRSKEEALAAKLVNKKNLRELTFE 620
           + L  FV G ++G ++ ELGNL NL G +++S+LE+V++ ++A  A L  K NL  L+  
Sbjct: 632 KTLGQFVVGRKKGYQLGELGNL-NLYGSIKISHLERVKNDKDAKEANLSAKGNLHSLSMS 691

Query: 621 WSIDILRECSSYNDFEVLEGLQPPKNLSSLKITNFGGKFLPA---ATFVENLVFLCLYGC 680
           W+ +         + +VLE L+P  NL+SLKI  F G  LP     + ++N+V + +   
Sbjct: 692 WN-NFGPHIYESEEVKVLEALKPHSNLTSLKIYGFRGIHLPEWMNHSVLKNIVSILISNF 751

Query: 681 TKCERLPMLGQLANLQELSICF----MDSVRSIGSEFY-GIDSNRRGYFPKLKKFDFCWM 740
             C  LP  G L  L+ L + +    ++ V  +  + + G  +  R  FP L+K D    
Sbjct: 752 RNCSCLPPFGDLPCLESLELHWGSADVEYVEEVDIDVHSGFPTRIR--FPSLRKLDI--- 811

Query: 741 CNLEQWELEVANHESNHFGSLQTLKLDRCGKLTKLPNGLECCKSVHEVIISNCPNLTL-- 800
                W+          FGSL+       G L K   G E    + E+II  CP LTL  
Sbjct: 812 -----WD----------FGSLK-------GLLKK--EGEEQFPVLEEMIIHECPFLTLSS 871

Query: 801 NVEEMHNLSVLLIDGLKFLPKGLALH-PNLKTIMIKGCIEDYDYSPFLNLPSLTKLYLND 860
           N+  + +L +         P+ +  +  NLK + I  C                      
Sbjct: 872 NLRALTSLRICYNKVATSFPEEMFKNLANLKYLTISRC---------------------- 931

Query: 861 GLGNATQLPKQLQHLTALKILAIENFYGIEVLP-EWLRKLTCLETLDLVRCKNLKRLPSR 920
              N  +LP  L  L ALK L I+    +E LP E L  L+ L  L +  C  LK LP  
Sbjct: 932 --NNLKELPTSLASLNALKSLKIQLCCALESLPEEGLEGLSSLTELFVEHCNMLKCLPE- 964

Query: 921 GAMRCLTKLKDFKVIACPLLLLGGQ--ADQEGAKYLHIP 939
             ++ LT L   K+  CP L+   +    ++  K  HIP
Sbjct: 992 -GLQHLTTLTSLKIRGCPQLIKRCEKGIGEDWHKISHIP 964

BLAST of Csa4G016360 vs. Swiss-Prot
Match: RGA1_SOLBU (Putative disease resistance protein RGA1 OS=Solanum bulbocastanum GN=RGA1 PE=2 SV=2)

HSP 1 Score: 276.2 bits (705), Expect = 1.4e-72
Identity = 246/771 (31.91%), Postives = 374/771 (48.51%), Query Frame = 1

Query: 159 ETTSILNFDVVGRETEVLDILR-----LVIDSSSNEYELPLLIVPILWEELEDCLKEIVG 218
           E  S+ + D+   + ++ ++L      LV+D   NE +         W  L   LK  VG
Sbjct: 231 EGKSLSDMDLAPLQKKLQELLNGKRYFLVLDDVWNEDQHK-------WANLRAVLK--VG 290

Query: 219 KFGITIIVTTRLDEVANIMGTVSGYRLEKLPEDHCWSLFKRSANANGVKMTPKLEAIRIK 278
             G  ++ TTRL++V +IMGT+  Y L  L  + CW LF + A  +  ++ P L AI  +
Sbjct: 291 ASGAFVLTTTRLEKVGSIMGTLQPYELSNLSPEDCWFLFMQRAFGHQEEINPNLMAIGKE 350

Query: 279 LLQKIDGIPLVAKVLGGAVEFEGDLDRWETTLESIVREIPMKQKSYVLSILQLSVDRLPF 338
           +++K  G+PL AK LGG + F+ +   WE   +S +  +P  + S +L  L+LS   LP 
Sbjct: 351 IVKKCGGVPLAAKTLGGILRFKREEREWEHVRDSPIWNLPQDESS-ILPALRLSYHHLPL 410

Query: 339 VEKQCFAYCSIFPKDCEVVKENLIRMWIAQGFIQPTEGENTMEDLGEGHFNFLLSRSLFQ 398
             +QCF YC++FPKD ++ KENLI  W+A GF+  ++G   +ED+G   +N L  RS FQ
Sbjct: 411 DLRQCFVYCAVFPKDTKMAKENLIAFWMAHGFLL-SKGNLELEDVGNEVWNELYLRSFFQ 470

Query: 399 DVVKDKYGRITHFKMHDLIHDVALAILSTRQKSVLDPTHWNGKTSRKLRTLLYNNQEIHH 458
           ++  +  G+ T+FKMHDLIHD+A ++ S    S  +    N      + ++ +      +
Sbjct: 471 EIEVES-GK-TYFKMHDLIHDLATSLFSANTSS-SNIREINANYDGYMMSIGFAEVVSSY 530

Query: 459 K---VADCVFLRVLEVNSLHMMNNLPDFIAKLKHLRYLDIS-SCSMWVMPHSVTTLFNLQ 518
               +   V LRVL + + + +N LP  I  L HLRYLD+S +  +  +P  +  L NLQ
Sbjct: 531 SPSLLQKFVSLRVLNLRNSN-LNQLPSSIGDLVHLRYLDLSGNFRIRNLPKRLCKLQNLQ 590

Query: 519 TLKL---GSIENLPMNLRNLVRLRHLEFHVYYNTRKMPSHMGELIHLQILSWFVAGFEEG 578
           TL L    S+  LP     L  LR+L       T   P  +G L  L+ LS FV G  +G
Sbjct: 591 TLDLHYCDSLSCLPKQTSKLGSLRNLLLDGCSLT-STPPRIGLLTCLKSLSCFVIGKRKG 650

Query: 579 CKIEELGNLKNLKGQLQLSNLEQVRSKEEALAAKLVNKKNLRELTFEWSIDILRECSSYN 638
            ++ EL NL NL G + ++ L++V+   +A  A L  K NL  L   W +D         
Sbjct: 651 HQLGELKNL-NLYGSISITKLDRVKKDTDAKEANLSAKANLHSLCLSWDLD----GKHRY 710

Query: 639 DFEVLEGLQPPKNLSSLKITNFGGKFLP---AATFVENLVFLCLYGCTKCERLPMLGQLA 698
           D EVLE L+P  NL  L+I  FGG  LP     + ++N+V + + GC  C  LP  G+L 
Sbjct: 711 DSEVLEALKPHSNLKYLEINGFGGIRLPDWMNQSVLKNVVSIRIRGCENCSCLPPFGELP 770

Query: 699 NLQELSICFMDSVRSIGSEFYGIDSNRRGYFPKLKK---FDFCWMCNLEQWELEVANHES 758
            L+ L +       S   E Y  D+   G FP L+K   +DF  +  L + E E      
Sbjct: 771 CLESLEL----HTGSADVE-YVEDNVHPGRFPSLRKLVIWDFSNLKGLLKMEGE------ 830

Query: 759 NHFGSLQTLKLDRCGKLTKLPNGLECCKSVHEVI--------ISNCPNLTLNVEEMHNLS 818
             F  L+ +    C  +  +P  L   K++  ++        ISN   LT +++   N+ 
Sbjct: 831 KQFPVLEEMTFYWC-PMFVIPT-LSSVKTLKVIVTDATVLRSISNLRALT-SLDISDNVE 890

Query: 819 VLLIDGLKFLPKGLALHPNLKTIMIKGCIEDYDYSPFLNLPSLTKLYLNDGLGNATQLPK 878
              +    F  K LA    LK    +   E       LN     K    D L     LP+
Sbjct: 891 ATSLPEEMF--KSLANLKYLKISFFRNLKELPTSLASLNALKSLKFEFCDAL---ESLPE 950

Query: 879 Q-LQHLTALKILAIENFYGIEVLPEWLRKLTCLETLDLVRCKNLKRLPSRG 903
           + ++ LT+L  L++ N   ++ LPE L+ LT L TL + +C  + +   RG
Sbjct: 951 EGVKGLTSLTELSVSNCMMLKCLPEGLQHLTALTTLTITQCPIVFKRCERG 961

HSP 2 Score: 46.6 bits (109), Expect = 1.8e-03
Identity = 33/92 (35.87%), Postives = 48/92 (52.17%), Query Frame = 1

Query: 831 NLPSLTKLYLNDGLGNATQLPKQL-QHLTALKILAIENFYGIEVLPEWLRKLTCLETLDL 890
           NL +LT L ++D +  AT LP+++ + L  LK L I  F  ++ LP  L  L  L++L  
Sbjct: 841 NLRALTSLDISDNV-EATSLPEEMFKSLANLKYLKISFFRNLKELPTSLASLNALKSLKF 900

Query: 891 VRCKNLKRLPSRGAMRCLTKLKDFKVIACPLL 922
             C  L+ LP  G ++ LT L +  V  C +L
Sbjct: 901 EFCDALESLPEEG-VKGLTSLTELSVSNCMML 930

HSP 3 Score: 42.7 bits (99), Expect = 2.6e-02
Identity = 32/95 (33.68%), Postives = 44/95 (46.32%), Query Frame = 1

Query: 826 YSPFLNLPSLTKLYLNDGLGNATQLPKQLQHLTALKILAIENFYGIEVLPEWLRKLTCLE 885
           YSP L    ++   LN    N  QLP  +  L  L+ L +   + I  LP+ L KL  L+
Sbjct: 516 YSPSLLQKFVSLRVLNLRNSNLNQLPSSIGDLVHLRYLDLSGNFRIRNLPKRLCKLQNLQ 575

Query: 886 TLDLVRCKNLKRLPSRGAMRCLTKLKDFKVIACPL 921
           TLDL  C +L  LP + +   L  L++  +  C L
Sbjct: 576 TLDLHYCDSLSCLPKQTSK--LGSLRNLLLDGCSL 608

BLAST of Csa4G016360 vs. Swiss-Prot
Match: RGA4_SOLBU (Putative disease resistance protein RGA4 OS=Solanum bulbocastanum GN=RGA4 PE=2 SV=1)

HSP 1 Score: 266.9 bits (681), Expect = 8.4e-70
Identity = 245/772 (31.74%), Postives = 368/772 (47.67%), Query Frame = 1

Query: 194 LLIVPILW-EELEDCLKE----IVGKFGITIIVTTRLDEVANIMGTVSGYRLEKLPEDHC 253
           LL++  +W ++LE   K      VG  G +I+ TTRL++V +IMGT+  Y L  L     
Sbjct: 259 LLVLDDVWNDDLEKWAKLRAVLTVGARGASILATTRLEKVGSIMGTLQPYHLSNLSPHDS 318

Query: 254 WSLFKRSANANGVKMTPKLEAIRIKLLQKIDGIPLVAKVLGGAVEFEGDLDRWETTLESI 313
             LF + A     +  P L AI  ++++K  G+PL AK LGG + F+ +   WE   ++ 
Sbjct: 319 LLLFMQRAFGQQKEANPNLVAIGKEIVKKCGGVPLAAKTLGGLLRFKREESEWEHVRDNE 378

Query: 314 VREIPMKQKSYVLSILQLSVDRLPFVEKQCFAYCSIFPKDCEVVKENLIRMWIAQGFIQP 373
           +  +P  + S +L  L+LS   LP   +QCFAYC++FPKD +++KENLI +W+A GF+  
Sbjct: 379 IWSLPQDESS-ILPALRLSYHHLPLDLRQCFAYCAVFPKDTKMIKENLITLWMAHGFLL- 438

Query: 374 TEGENTMEDLGEGHFNFLLSRSLFQDVVKDKYGRITHFKMHDLIHDVALAILST------ 433
           ++G   +ED+G   +N L  RS FQ+ ++ K G  T+FK+HDLIHD+A ++ S       
Sbjct: 439 SKGNLELEDVGNEVWNELYLRSFFQE-IEAKSGN-TYFKIHDLIHDLATSLFSASASCGN 498

Query: 434 -RQKSVLDPTHWNGKTSRKLRTLLYNNQEIHHKVADCVFLRVLEVNSLHMMNNLPDFIAK 493
            R+ +V D  H     +  +      +      +   V LRVL + S   +  LP  I  
Sbjct: 499 IREINVKDYKH-----TVSIGFAAVVSSYSPSLLKKFVSLRVLNL-SYSKLEQLPSSIGD 558

Query: 494 LKHLRYLDISSCSMWVMPHSVTTLFNLQTLKLG---SIENLPMNLRNLVRLRHLEFHVYY 553
           L HLRYLD+S  +   +P  +  L NLQTL +    S+  LP     L  LRHL      
Sbjct: 559 LLHLRYLDLSCNNFRSLPERLCKLQNLQTLDVHNCYSLNCLPKQTSKLSSLRHLVVDGCP 618

Query: 554 NTRKMPSHMGELIHLQILSWFVAGFEEGCKIEELGNLKNLKGQLQLSNLEQVRSKEEALA 613
            T   P  +G L  L+ L +F+ G ++G ++ EL NL NL G + +++LE+V++  +A  
Sbjct: 619 LT-STPPRIGLLTCLKTLGFFIVGSKKGYQLGELKNL-NLCGSISITHLERVKNDTDA-E 678

Query: 614 AKLVNKKNLRELTFEWSIDILRECSSYNDFEVLEGLQPPKNLSSLKITNFGGKFLPA--- 673
           A L  K NL+ L+  W  D      S  + +VLE L+P  NL  L+I  FGG   P+   
Sbjct: 679 ANLSAKANLQSLSMSWDNDGPNRYES-KEVKVLEALKPHPNLKYLEIIAFGGFRFPSWIN 738

Query: 674 ATFVENLVFLCLYGCTKCERLPMLGQLANLQELSICFMDS-VRSIGSEFYGIDSNRRGYF 733
            + +E ++ + +  C  C  LP  G+L  L+ L +    + V  +  +      + R  F
Sbjct: 739 HSVLEKVISVRIKSCKNCLCLPPFGELPCLENLELQNGSAEVEYVEEDDVHSRFSTRRSF 798

Query: 734 PKLKKFDFCWMCNLEQWELEVANHESNHFGSLQTLKLDRCGKLTKLPNGLECCKSVHEVI 793
           P LKK        L  W           F SL+ L  +          G E    + E+ 
Sbjct: 799 PSLKK--------LRIW----------FFRSLKGLMKE---------EGEEKFPMLEEMA 858

Query: 794 ISNCPNLTLNVEEMHNLSVLLIDGLKFLPKGLALHPNLKTIMIKGCIEDYDYSP-----F 853
           I  CP        + ++  L + G     +GL+   NL T+       +Y  +      F
Sbjct: 859 ILYCPLFVF--PTLSSVKKLEVHG-NTNTRGLSSISNLSTLTSLRIGANYRATSLPEEMF 918

Query: 854 LNLPSLTKLYLNDGLGNATQLPKQLQHLTALKILAIENFYGIEVLPE-WLRKLTCLETLD 913
            +L +L  L   D   N   LP  L  L ALK L IE+   +E  PE  L  LT L  L 
Sbjct: 919 TSLTNLEFLSFFD-FKNLKDLPTSLTSLNALKRLQIESCDSLESFPEQGLEGLTSLTQLF 978

Query: 914 LVRCKNLKRLPSRGAMRCLTKLKDFKVIACPLL--LLGGQADQEGAKYLHIP 939
           +  CK LK LP    ++ LT L +  V  CP +      +  ++  K  HIP
Sbjct: 979 VKYCKMLKCLPE--GLQHLTALTNLGVSGCPEVEKRCDKEIGEDWHKIAHIP 983

BLAST of Csa4G016360 vs. Swiss-Prot
Match: R13L1_ARATH (Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana GN=RPPL1 PE=3 SV=1)

HSP 1 Score: 254.6 bits (649), Expect = 4.3e-66
Identity = 229/782 (29.28%), Postives = 358/782 (45.78%), Query Frame = 1

Query: 176 LDILRLVIDSSSNEYELP-LLIVPILWEELE---DCLKE--IVGKFGITIIVTTRLDEVA 235
           LD+L++ +        LP LL++  LW E     D L++  I    G  I+VTTR   VA
Sbjct: 261 LDVLQVKLKERLTGTGLPFLLVLDDLWNENFADWDLLRQPFIHAAQGSQILVTTRSQRVA 320

Query: 236 NIMGTVSGYRLEKLPEDHCWSLFKRSANANGVK-MTPKLEAIRIKLLQKIDGIPLVAKVL 295
           +IM  V  + L+ L +  CWSLF ++   N    +  ++  +  +++ K  G+PL  K L
Sbjct: 321 SIMCAVHVHNLQPLSDGDCWSLFMKTVFGNQEPCLNREIGDLAERIVHKCRGLPLAVKTL 380

Query: 296 GGAVEFEGDLDRWETTLESIVREIPMKQKSYVLSILQLSVDRLPFVEKQCFAYCSIFPKD 355
           GG + FEG +  WE  L S + ++P   KS +L +L++S   LP   K+CFAYCSIFPK 
Sbjct: 381 GGVLRFEGKVIEWERVLSSRIWDLPA-DKSNLLPVLRVSYYYLPAHLKRCFAYCSIFPKG 440

Query: 356 CEVVKENLIRMWIAQGFIQPTEGENTMED---------LGEGHFNFLLSRSLFQDVVKD- 415
               K+ ++ +W+A+GF+Q T     +E+                   +R +  D + + 
Sbjct: 441 HAFEKDKVVLLWMAEGFLQQTRSSKNLEELGNEYFSELESRSLLQKTKTRYIMHDFINEL 500

Query: 416 ---KYGRITHFKMHD---LIHDVALAILSTRQKSVLDPTHWNGKTSRK-LRTLL------ 475
                G  +  K  D   L        LS  + +  +P  +      K LRT L      
Sbjct: 501 AQFASGEFSS-KFEDGCKLQVSERTRYLSYLRDNYAEPMEFEALREVKFLRTFLPLSLTN 560

Query: 476 -----YNNQEIHHKVADCVF-LRVLEVNSLHMMNNLPDFIAKLKHLRYLDISSCSMWVMP 535
                  +Q +  K+   +  LRVL ++   +    PDF   + H R+LD+S   +  +P
Sbjct: 561 SSRSCCLDQMVSEKLLPTLTRLRVLSLSHYKIARLPPDFFKNISHARFLDLSRTELEKLP 620

Query: 536 HSVTTLFNLQTLKLG---SIENLPMNLRNLVRLRHLEFHVYYNTRKMPSHMGELIHLQIL 595
            S+  ++NLQTL L    S++ LP ++ NL+ LR+L+  +    R+MP   G L  LQ L
Sbjct: 621 KSLCYMYNLQTLLLSYCSSLKELPTDISNLINLRYLDL-IGTKLRQMPRRFGRLKSLQTL 680

Query: 596 SWFVAGFEEGCKIEELGNLKNLKGQLQLSNLEQVRSKEEALAAKLVNKKNLRELTFEW-- 655
           + F     +G +I ELG L +L G+L++  L++V    +A  A L +KK+LRE+ F W  
Sbjct: 681 TTFFVSASDGSRISELGGLHDLHGKLKIVELQRVVDVADAAEANLNSKKHLREIDFVWRT 740

Query: 656 ----SIDILRECSSYNDFEVLEGLQPPKNLSSLKITNFGGKFLP---AATFVENLVFLCL 715
               S +      + N+ EV E L+P +++  L I  + G+  P   +      +V + L
Sbjct: 741 GSSSSENNTNPHRTQNEAEVFEKLRPHRHIEKLAIERYKGRRFPDWLSDPSFSRIVCIRL 800

Query: 716 YGCTKCERLPMLGQLANLQELSICFMDSVRSIGSEFYGIDSNRRGY----FPKLKKFDFC 775
             C  C  LP LGQL  L+EL I  M  ++SIG +FY  D   R      F  L+   F 
Sbjct: 801 RECQYCTSLPSLGQLPCLKELHISGMVGLQSIGRKFYFSDQQLRDQDQQPFRSLETLRFD 860

Query: 776 WMCNLEQWELEVANHESNHFGSLQTLKLDRCGKLT-KLPNGLECCKSVHEVIISNCPNLT 835
            + + ++W L+V     + F SL+ L + RC +LT  LP  L    S+H   I  C  L 
Sbjct: 861 NLPDWQEW-LDVRVTRGDLFPSLKKLFILRCPELTGTLPTFLPSLISLH---IYKCGLLD 920

Query: 836 LNVEEMHNLSVLLIDGLKFLPKGLALHPNLKTIMIKGCIEDYDYSPFLNLPSLTKLYLND 895
              +  H  S                + NL+T+ IK   +           +L K  LN 
Sbjct: 921 FQPDH-HEYS----------------YRNLQTLSIKSSCD-----------TLVKFPLN- 980

Query: 896 GLGNATQLPKQLQHLTALKILAIENFYGIEVLPEWLRKLTCLETLDLVRCKNLKRLPSRG 905
                        +L  L++    + Y +E+  E LR    L  L +  C+NL+ LP   
Sbjct: 981 ----------HFANLDKLEVDQCTSLYSLELSNEHLRGPNALRNLRINDCQNLQLLPKLN 996

HSP 2 Score: 50.4 bits (119), Expect = 1.2e-04
Identity = 45/174 (25.86%), Postives = 80/174 (45.98%), Query Frame = 1

Query: 32  EDELSNLSKWLLDAGALLRDIDREILRKESVKRWADGLEDIVSEAEDLLDELAYEDLRRK 91
           E+ L  LS  LL   A+L D + + +    V++W + L D+V  AED LD++A E LR  
Sbjct: 36  ENLLERLSTALLTITAVLIDAEEKQITNPVVEKWVNELRDVVYHAEDALDDIATEALRLN 95

Query: 92  VETSSRVCNNFK-------FSSVLNPLVRHDMACKMKKITKMLKQHYRNSAPLGLVGKES 151
           +   S   N  +           L+    H +  +++K+T  L++       LGL     
Sbjct: 96  IGAESSSSNRLRQLRGRMSLGDFLDGNSEH-LETRLEKVTIRLERLASQRNILGL----- 155

Query: 152 MEKEDGGNNLRQIRETTSILN-FDVVGRETEVLDILRLVIDSSSNEYELPLLIV 198
             KE      +Q   TTS+++  +V GR+ +  +I+R +I  +  +  + ++ +
Sbjct: 156 --KELTAMIPKQRLPTTSLVDESEVFGRDDDKDEIMRFLIPENGKDNGITVVAI 201

BLAST of Csa4G016360 vs. TrEMBL
Match: A0A0A0KXG0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G016360 PE=3 SV=1)

HSP 1 Score: 1928.7 bits (4995), Expect = 0.0e+00
Identity = 958/958 (100.00%), Postives = 958/958 (100.00%), Query Frame = 1

Query: 1   MAEFLWTFAVEETLKRTVNVAAQKISLVWGLEDELSNLSKWLLDAGALLRDIDREILRKE 60
           MAEFLWTFAVEETLKRTVNVAAQKISLVWGLEDELSNLSKWLLDAGALLRDIDREILRKE
Sbjct: 1   MAEFLWTFAVEETLKRTVNVAAQKISLVWGLEDELSNLSKWLLDAGALLRDIDREILRKE 60

Query: 61  SVKRWADGLEDIVSEAEDLLDELAYEDLRRKVETSSRVCNNFKFSSVLNPLVRHDMACKM 120
           SVKRWADGLEDIVSEAEDLLDELAYEDLRRKVETSSRVCNNFKFSSVLNPLVRHDMACKM
Sbjct: 61  SVKRWADGLEDIVSEAEDLLDELAYEDLRRKVETSSRVCNNFKFSSVLNPLVRHDMACKM 120

Query: 121 KKITKMLKQHYRNSAPLGLVGKESMEKEDGGNNLRQIRETTSILNFDVVGRETEVLDILR 180
           KKITKMLKQHYRNSAPLGLVGKESMEKEDGGNNLRQIRETTSILNFDVVGRETEVLDILR
Sbjct: 121 KKITKMLKQHYRNSAPLGLVGKESMEKEDGGNNLRQIRETTSILNFDVVGRETEVLDILR 180

Query: 181 LVIDSSSNEYELPLLIVPILWEELEDCLKEIVGKFGITIIVTTRLDEVANIMGTVSGYRL 240
           LVIDSSSNEYELPLLIVPILWEELEDCLKEIVGKFGITIIVTTRLDEVANIMGTVSGYRL
Sbjct: 181 LVIDSSSNEYELPLLIVPILWEELEDCLKEIVGKFGITIIVTTRLDEVANIMGTVSGYRL 240

Query: 241 EKLPEDHCWSLFKRSANANGVKMTPKLEAIRIKLLQKIDGIPLVAKVLGGAVEFEGDLDR 300
           EKLPEDHCWSLFKRSANANGVKMTPKLEAIRIKLLQKIDGIPLVAKVLGGAVEFEGDLDR
Sbjct: 241 EKLPEDHCWSLFKRSANANGVKMTPKLEAIRIKLLQKIDGIPLVAKVLGGAVEFEGDLDR 300

Query: 301 WETTLESIVREIPMKQKSYVLSILQLSVDRLPFVEKQCFAYCSIFPKDCEVVKENLIRMW 360
           WETTLESIVREIPMKQKSYVLSILQLSVDRLPFVEKQCFAYCSIFPKDCEVVKENLIRMW
Sbjct: 301 WETTLESIVREIPMKQKSYVLSILQLSVDRLPFVEKQCFAYCSIFPKDCEVVKENLIRMW 360

Query: 361 IAQGFIQPTEGENTMEDLGEGHFNFLLSRSLFQDVVKDKYGRITHFKMHDLIHDVALAIL 420
           IAQGFIQPTEGENTMEDLGEGHFNFLLSRSLFQDVVKDKYGRITHFKMHDLIHDVALAIL
Sbjct: 361 IAQGFIQPTEGENTMEDLGEGHFNFLLSRSLFQDVVKDKYGRITHFKMHDLIHDVALAIL 420

Query: 421 STRQKSVLDPTHWNGKTSRKLRTLLYNNQEIHHKVADCVFLRVLEVNSLHMMNNLPDFIA 480
           STRQKSVLDPTHWNGKTSRKLRTLLYNNQEIHHKVADCVFLRVLEVNSLHMMNNLPDFIA
Sbjct: 421 STRQKSVLDPTHWNGKTSRKLRTLLYNNQEIHHKVADCVFLRVLEVNSLHMMNNLPDFIA 480

Query: 481 KLKHLRYLDISSCSMWVMPHSVTTLFNLQTLKLGSIENLPMNLRNLVRLRHLEFHVYYNT 540
           KLKHLRYLDISSCSMWVMPHSVTTLFNLQTLKLGSIENLPMNLRNLVRLRHLEFHVYYNT
Sbjct: 481 KLKHLRYLDISSCSMWVMPHSVTTLFNLQTLKLGSIENLPMNLRNLVRLRHLEFHVYYNT 540

Query: 541 RKMPSHMGELIHLQILSWFVAGFEEGCKIEELGNLKNLKGQLQLSNLEQVRSKEEALAAK 600
           RKMPSHMGELIHLQILSWFVAGFEEGCKIEELGNLKNLKGQLQLSNLEQVRSKEEALAAK
Sbjct: 541 RKMPSHMGELIHLQILSWFVAGFEEGCKIEELGNLKNLKGQLQLSNLEQVRSKEEALAAK 600

Query: 601 LVNKKNLRELTFEWSIDILRECSSYNDFEVLEGLQPPKNLSSLKITNFGGKFLPAATFVE 660
           LVNKKNLRELTFEWSIDILRECSSYNDFEVLEGLQPPKNLSSLKITNFGGKFLPAATFVE
Sbjct: 601 LVNKKNLRELTFEWSIDILRECSSYNDFEVLEGLQPPKNLSSLKITNFGGKFLPAATFVE 660

Query: 661 NLVFLCLYGCTKCERLPMLGQLANLQELSICFMDSVRSIGSEFYGIDSNRRGYFPKLKKF 720
           NLVFLCLYGCTKCERLPMLGQLANLQELSICFMDSVRSIGSEFYGIDSNRRGYFPKLKKF
Sbjct: 661 NLVFLCLYGCTKCERLPMLGQLANLQELSICFMDSVRSIGSEFYGIDSNRRGYFPKLKKF 720

Query: 721 DFCWMCNLEQWELEVANHESNHFGSLQTLKLDRCGKLTKLPNGLECCKSVHEVIISNCPN 780
           DFCWMCNLEQWELEVANHESNHFGSLQTLKLDRCGKLTKLPNGLECCKSVHEVIISNCPN
Sbjct: 721 DFCWMCNLEQWELEVANHESNHFGSLQTLKLDRCGKLTKLPNGLECCKSVHEVIISNCPN 780

Query: 781 LTLNVEEMHNLSVLLIDGLKFLPKGLALHPNLKTIMIKGCIEDYDYSPFLNLPSLTKLYL 840
           LTLNVEEMHNLSVLLIDGLKFLPKGLALHPNLKTIMIKGCIEDYDYSPFLNLPSLTKLYL
Sbjct: 781 LTLNVEEMHNLSVLLIDGLKFLPKGLALHPNLKTIMIKGCIEDYDYSPFLNLPSLTKLYL 840

Query: 841 NDGLGNATQLPKQLQHLTALKILAIENFYGIEVLPEWLRKLTCLETLDLVRCKNLKRLPS 900
           NDGLGNATQLPKQLQHLTALKILAIENFYGIEVLPEWLRKLTCLETLDLVRCKNLKRLPS
Sbjct: 841 NDGLGNATQLPKQLQHLTALKILAIENFYGIEVLPEWLRKLTCLETLDLVRCKNLKRLPS 900

Query: 901 RGAMRCLTKLKDFKVIACPLLLLGGQADQEGAKYLHIPAYLCHVYQSRGSPLSKTSSI 959
           RGAMRCLTKLKDFKVIACPLLLLGGQADQEGAKYLHIPAYLCHVYQSRGSPLSKTSSI
Sbjct: 901 RGAMRCLTKLKDFKVIACPLLLLGGQADQEGAKYLHIPAYLCHVYQSRGSPLSKTSSI 958

BLAST of Csa4G016360 vs. TrEMBL
Match: A0A0A0KST5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G266890 PE=3 SV=1)

HSP 1 Score: 709.9 bits (1831), Expect = 4.2e-201
Identity = 405/755 (53.64%), Postives = 517/755 (68.48%), Query Frame = 1

Query: 200  LWEELEDCLKEIVGKFGITIIVTTRLDEVANIMGTVSGYRLEKLPEDHCWSLFKRSANAN 259
            LW+EL   LK I GK G +I+VTTR  EVA ++ TV  Y L+KL +DHCW+L K+SANAN
Sbjct: 286  LWDELIGNLKYIAGKSGNSIMVTTRSVEVATMVKTVPIYHLKKLSDDHCWALLKKSANAN 345

Query: 260  GVKMTPKLEAIRIKLLQKIDGIPLVAKVLGGAVEFE-GDLDRWETTLESIVREIPMKQKS 319
             ++M  KLE  +  L++KI G+PL+AKVLGGAV+FE G  + W   +ES  R I ++ K 
Sbjct: 346  QLQMNSKLENTKNILVRKIGGVPLIAKVLGGAVKFEEGGSESWMAKIESFARNISIEDKD 405

Query: 320  YVLSILQLSVDRLPFVE-KQCFAYCSIFPKDCEVVKENLIRMWIAQGFIQPT-EGEN-TM 379
            +VLSIL+LSV+ LP    KQCFAYCS FP+D E  K+  I+MWIA+GFIQP  E EN TM
Sbjct: 406  FVLSILKLSVESLPHSALKQCFAYCSNFPQDYEFDKDEAIQMWIAEGFIQPEQERENLTM 465

Query: 380  EDLGEGHFNFLLSRSLFQDVVKDKYGRITHFKMHDLIHDVALAILSTRQKSVLDPTHWNG 439
            E++GE + NFLLSRSLF+D +K   GRI  FK+HDL+HD+A AI S   K   +P  WNG
Sbjct: 466  ENIGEEYLNFLLSRSLFEDAIKYD-GRIVTFKIHDLMHDIACAI-SNHHKMDSNPISWNG 525

Query: 440  KTSRKLRTLLYNNQEIHHKV-ADCVFLRVLEVNSLHMMNNLPDFIAKLKHLRYLDISSCS 499
            K++RKLRTL+  N+E  HK+  D + LRVL +      N L   + KL HLRYLDIS+C+
Sbjct: 526  KSTRKLRTLICENEEAFHKIQTDIICLRVLVLKWFDT-NTLSTIMDKLIHLRYLDISNCN 585

Query: 500  M-WVMPHSVTTLFNLQTLKLGSIE-NLPMNLRNLVRLRHLEFHVYYNTRKMPSHMGELIH 559
            +  ++  S+  L+NLQTLKLG IE +LP NLRNLV LRHLEF  +++  +MPSHMG +IH
Sbjct: 586  INKLLRDSICALYNLQTLKLGYIECDLPKNLRNLVNLRHLEFKKFFDMGQMPSHMGNMIH 645

Query: 560  LQILSWFVAGFEEGCKIEELGNLKNLKGQLQLSNLEQVRSKEEALAAKLVNKKNLRELTF 619
            LQ LS FV G E+GCKI+ELG LK+LKG L L NL+ V++K+EA+AAKLV KK LR L F
Sbjct: 646  LQTLSEFVVGLEKGCKIDELGPLKDLKGTLTLKNLQNVQNKDEAMAAKLVEKKYLRHLIF 705

Query: 620  EWSIDIL--RECSSYNDFEVLEGLQPPKNLSSLKITNFGGKFLPAATFVENLVFLCLYGC 679
            +W +++    E    ++ +VLEGLQP KN+ SL I  F G+ L    FVENLV + L  C
Sbjct: 706  QWFLNLYDRGEYDEDDNKQVLEGLQPHKNVQSLDIRGFQGRVLNNNIFVENLVEIRLVDC 765

Query: 680  TKCERLPMLGQLANLQELSICFMDSVRSIGSEFYGIDSNRR--GYFPKLKKFDFCWMCNL 739
             +CE LPMLGQL NL++L I  M+SVRSIGSEFYG+D N R    FP+L KF  C +  L
Sbjct: 766  GRCEVLPMLGQLPNLKKLEIISMNSVRSIGSEFYGVDCNDRNSSAFPQLNKFHICGLKKL 825

Query: 740  EQWELEVANHESNHFGSLQTLKLDRCGKLTKLPNGLECCKSVHEVIISNCPNLTLNVEEM 799
            +QW+ E     SN FG L+ L L  C +L KLP+GLE C S+  + I  CPNL LNV+ +
Sbjct: 826  QQWD-EATVFASNRFGCLKELILSGCHQLAKLPSGLEGCYSIEYLAIDGCPNLMLNVQNL 885

Query: 800  HNLSVLLIDGLKFLPKGLALHPNLKTIMIKGCIEDYDYSPFLNLPS-LTKLYLND---GL 859
            +NL  L I GLK LP       NLK + I GC+++Y++SPF++L S L +L L D     
Sbjct: 886  YNLYHLDIRGLKRLPDEFGKLTNLKKLRIGGCMQNYEFSPFIHLSSQLVELELTDDGSSG 945

Query: 860  GNATQLPKQLQHLTALKILAIENFYGIEVLPEWLRKLTCLETLDLVRCKNLKRLPSRGAM 919
               TQLP+QLQHLT LK+L I +F  IEVLPEWL  LTCL TL  + CKNLK LPSR A+
Sbjct: 946  SETTQLPQQLQHLTNLKVLKIADFDDIEVLPEWLGNLTCLATLVFLECKNLKELPSREAI 1005

Query: 920  RCLTKLKDFKVIACPLLLLGGQADQEGAKYLHIPA 940
            + LTKL D  +  CP LLL G+ DQE AK  H+P+
Sbjct: 1006 QRLTKLDDLVIDGCPKLLL-GEGDQERAKLSHLPS 1035

BLAST of Csa4G016360 vs. TrEMBL
Match: A0A0A0KST5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G266890 PE=3 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 3.9e-42
Identity = 103/202 (50.99%), Postives = 135/202 (66.83%), Query Frame = 1

Query: 1   MAEFLWTFAVEETLKRTVNVAAQKISLVWGLEDELSNLSKWLLDAGALLRDIDREILRKE 60
           MAEFLWTFAV+E LK+ VN  A++ISL WGLE ELS+L KWLL A  +L DI+ +     
Sbjct: 1   MAEFLWTFAVQEVLKKIVNFGAEQISLAWGLEKELSHLKKWLLKAQTILADINTKKSHHH 60

Query: 61  SVKRWADGLEDIVSEAEDLLDELAYEDLRRKVETSSRVCNNFKFSSVLNP-----LVRHD 120
           SV  W + L DI+ EA+DLLDE+ YE +R+ VE + ++    K    ++P     L    
Sbjct: 61  SVGLWVEELHDIIYEADDLLDEIVYEQIRQTVEQTGKL---RKVRDSISPSKNSFLFGLK 120

Query: 121 MACKMKKITKMLKQHYRNSAPLGLVGKESMEKEDGGNNLRQIRETTSILNFDVVGRETEV 180
           MA KMKKITK L +HY  ++PLGLVG ES  + +    L QIRETTSIL+F+V GRE EV
Sbjct: 121 MAKKMKKITKTLYEHYCEASPLGLVGDESTTESEAA--LNQIRETTSILDFEVEGREAEV 180

Query: 181 LDILRLVIDSSSNEYELPLLIV 198
           L+IL+LVIDS+  ++   + IV
Sbjct: 181 LEILKLVIDSTDEDHISVISIV 197

HSP 2 Score: 687.2 bits (1772), Expect = 2.9e-194
Identity = 404/767 (52.67%), Postives = 521/767 (67.93%), Query Frame = 1

Query: 200  LWEELEDCLKEIVGKFGITIIVTTRLDEVANIMGTVSGYRLEKLPEDHCWSLFKRSANAN 259
            LW+EL  CLK I GK G TI+VTTR +EVA ++  +S YRL+KL  D CW+LFK SANAN
Sbjct: 287  LWDELRGCLKHIAGKPGNTIMVTTRNEEVATMVEPISIYRLKKLSNDQCWALFKESANAN 346

Query: 260  GVKMTPKLEAIRIKLLQKIDGIPLVAKVLGGAVEF-EGDLDR------WETTLESIVREI 319
             + M  KLE ++ +L++K+ G+PLVAKVLGGAV+F E +L+       W T +ESIVR I
Sbjct: 347  QLPMNSKLEIMKKELVRKMGGVPLVAKVLGGAVKFEETELEEEDHEISWMTKVESIVRNI 406

Query: 320  PMKQKSYVLSILQLSVDRLPF-VEKQCFAYCSIFPKDCEVVKENLIRMWIAQGFIQPTEG 379
             ++ K +VLSIL+LSVD LP  V KQC AYCS F +D +  K++LI+MWIAQGFIQP +G
Sbjct: 407  SLEDKDFVLSILKLSVDSLPNPVLKQCVAYCSNFSQDYDFQKDDLIKMWIAQGFIQPGQG 466

Query: 380  EN---TMEDLGEGHFNFLLSRSLFQDVVKDKYGRITHFKMHDLIHDVALAILSTRQKSVL 439
             +    MED+GE +FNFLLSRS+FQDV +D   RI  FKMHDL+HD+A AI S+ Q    
Sbjct: 467  RDKNLLMEDIGEQYFNFLLSRSIFQDVTRDANKRIVGFKMHDLMHDIACAI-SSHQNVES 526

Query: 440  DPTHWNGKTSRKLRTLLYNNQEIHH-KVADCVFLRVLEVNSLHMMNNLPDFIAKLKHLRY 499
            +P + +GK+ RKLRTL+ N++ I++    D V LRVL+V       +L   I KL HLRY
Sbjct: 527  NPNNLSGKSVRKLRTLICNDEVINYLNQNDIVCLRVLKV-IFQSHTDLWIPIDKLIHLRY 586

Query: 500  LDISSCSM-WVMPHSVTTLFNLQTLKLGSIENLPMNLRNLVRLRHLEFHVYYNTRKMPSH 559
            LDIS CS+  ++  S++ L+NLQTLKLG    LP NLR LV LRHLEF ++ +T  MPS 
Sbjct: 587  LDISECSINKLLLESLSLLYNLQTLKLGQ-SGLPKNLRKLVNLRHLEFKMFGDT-AMPSD 646

Query: 560  MGELIHLQILSWFVAGFEEGCKIEELGNLKNLKGQLQLSNLEQVRSKEEALAAKLVNKKN 619
            MG LIHLQ LS F+ GFE+GCKIEELG LKNLKG+L L+NL +V++K+EA+AAKLV KKN
Sbjct: 647  MGNLIHLQSLSGFLVGFEKGCKIEELGPLKNLKGKLTLTNLWRVQNKDEAMAAKLVEKKN 706

Query: 620  LRELTFEWSIDILRECSSYND--FEVLEGLQPPKNLSSLKITNFGGKFLPAATFVENLVF 679
            LR L   W  +  +      D   +VLEGLQP KNL SL+I  F GK LP   FVENLV 
Sbjct: 707  LRHLNL-WFFETDKRGEDDEDGIVQVLEGLQPHKNLQSLEILGFRGKVLPTGIFVENLVK 766

Query: 680  LCLYGCTKCERLPMLGQLANLQELSICFMDSVRSIGSEFYGIDSNRRG--YFPKLKKFDF 739
            + L    +CE LPMLGQL NL+EL I +M+SVRSIG+EFYG+DS+ +    FP+LKK   
Sbjct: 767  IRLGHFERCEVLPMLGQLPNLKELEIMYMESVRSIGNEFYGVDSSHQNSVAFPQLKKLSI 826

Query: 740  CWMCNLEQWELEVANHESNHFGSLQTLKLDRCGKLTKLPNGLECCKSVHEVIISNCPNLT 799
              M NLEQW+      ESN FG L+ +++ RC  L KLP+GLE C S+  + I  C NL 
Sbjct: 827  YEMMNLEQWDEATVVLESNLFGCLKEVRIRRCNPLAKLPSGLEGCHSLEYLSIRGCFNLM 886

Query: 800  LNVEEMHNLSVLLIDGLKFLPKGLALHPNLKTIMIKGCIEDYDYSPFLNLPS-LTKLYLN 859
            LNV+ +H L  L IDGLK LPKG+     LK + I GC+++Y++S  ++L S L +L L+
Sbjct: 887  LNVQNLHKLYHLEIDGLKRLPKGMDGLTRLKELKIGGCMQNYEFSSVIHLASQLVELELS 946

Query: 860  DGLGNA-TQLPKQLQHLTALKILAIENFYGIEVLPEWLRKLTCLETLDLVRCKNLKRLPS 919
               G+  TQLP+QLQHLT L++L I  F  IE LPEW+  L  L+TL    C  LK LPS
Sbjct: 947  GRYGSVDTQLPQQLQHLTNLQVLKITQFDCIEALPEWIGNLISLKTLKCSYCFKLKELPS 1006

Query: 920  RGAMRCLTKLKDFKVIACPLLLLGGQADQEGAKYLHIPAYLCHVYQS 948
            R A+  LTKL++  +  CP LL+ G+ DQE AK  H+P+   H  +S
Sbjct: 1007 REAILRLTKLENLDIFECPKLLV-GEGDQERAKLSHLPSKCVHKSES 1047

BLAST of Csa4G016360 vs. TrEMBL
Match: A0A0A0LBV0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G822360 PE=3 SV=1)

HSP 1 Score: 151.0 bits (380), Expect = 7.5e-33
Identity = 97/201 (48.26%), Postives = 132/201 (65.67%), Query Frame = 1

Query: 1   MAEFLWTFAVEETLKRTVNVAAQKISLVWGLEDELSNLSKWLLDAGALLRDIDREILRK- 60
           MAE + TF V+E LKR V   A++I + W LE+E+S L   L DA  +L DI+R+     
Sbjct: 1   MAE-IGTFVVQEVLKRIVKYGAEQIVVAWELENEVSLLKDKLHDADTILEDINRKKSHPG 60

Query: 61  ESVKRWADGLEDIVSEAEDLLDELAYEDLRRKVETS---SRVCNNFKFSSVLNPLVRHDM 120
            SVKRW + LEDIV EA+DLLDEL YE LRR VE +   S+V ++   SS+ + L R  M
Sbjct: 61  NSVKRWVEKLEDIVHEADDLLDELVYEHLRRTVEHTEKFSKVSDSIS-SSINSFLFRRKM 120

Query: 121 ACKMKKITKMLKQHYRNSAPLGLVGKESMEKEDGGNNLRQIRETTSILNFDVVGRETEVL 180
           A K+K IT  L QHY  ++  GLVG E++ + +    L QIRETTSIL+F V GRE EVL
Sbjct: 121 AKKIKNITDTLNQHYCAASAFGLVGVETVTEIELA--LNQIRETTSILDFQVEGREAEVL 180

Query: 181 DILRLVIDSSSNEYELPLLIV 198
           ++L+L ID S+NE+ + ++ +
Sbjct: 181 ELLKLAID-STNEHHMSVISI 196

HSP 2 Score: 664.5 bits (1713), Expect = 2.0e-187
Identity = 423/979 (43.21%), Postives = 594/979 (60.67%), Query Frame = 1

Query: 1   MAEFLWTFAVEETLKRTVNVAAQKISLVWGLEDELSNLSKWLLDAGALLRDIDREILRKE 60
           MAEFLWTFAV+E LK+ + +AA +I L WGL+ ELSNLS+WLL A A+L +I+R+ L   
Sbjct: 1   MAEFLWTFAVQEVLKKVLKLAADQIGLAWGLDKELSNLSQWLLKAEAILGEINRKKLHPS 60

Query: 61  SVKRWADGLEDIVSEAEDLLDELAYEDLRRKVETSSRVCNNFKFSSVLNPLVRHDMACKM 120
           SV+ W                    EDL+  V  +           +L+ LV  D+  K+
Sbjct: 61  SVRLW-------------------VEDLQLVVHEAD---------DLLDELVYEDLRTKV 120

Query: 121 KK--ITKMLKQHYRNSAPLGLVGKESMEKEDGGNNLRQIRETTS------ILNFDVVGRE 180
           +K  I K+ +    + + + ++ +  M K+     ++++R+  S      ++  + +  E
Sbjct: 121 EKGPINKV-RSSISSLSNIFIIFRFKMAKKIKAI-IQKLRKCYSEATPLGLVGEEFIQTE 180

Query: 181 TEVLDILRLVIDSSSNEYELPLLIVPILWEELEDCLKEIVGKFGITIIVTTRLDEVANIM 240
            ++  I   +  S  +++E       ++  E E  +  IV +     +V   +D V +I+
Sbjct: 181 NDLSQIRETI--SKLDDFE-------VVGREFE--VSSIVKQ-----VVDASIDNVTSIL 240

Query: 241 ---------GTVSGYRLEKLPEDHCWSLFKRSANANGVKMTPKLEAIRIKLLQKIDGIPL 300
                     T+S + L KL ++ CWSLFK+SANA+ +    +L+ ++ +L+ +  G PL
Sbjct: 241 PIVVGKIMESTLSSHHLGKLSDEQCWSLFKKSANADELPKNLELKDLQEELVTRFGGAPL 300

Query: 301 VAKVLGGAVEFEGDLDRWETTLESIVREIPMKQKSYVLSILQLSVDRLP-FVEKQCFAYC 360
           VA+VLGGA++FEG  ++W  +L +    IP++ +  VLS L+LSVDRLP F+ KQCFAYC
Sbjct: 301 VARVLGGALKFEGVYEKWVMSLRTTT-SIPLQDEDLVLSTLKLSVDRLPSFLLKQCFAYC 360

Query: 361 SIFPKDCEVVKENLIRMWIAQGFIQPTEGEN--TMEDLGEGHFNFLLSRSLFQDVVKDKY 420
           S FPK  +  KE LI MW+AQGFIQ  EG N  TME+ GE +FN LLSRSLFQD++KD  
Sbjct: 361 SNFPKGFKFKKEELIEMWMAQGFIQLHEGRNEITMEENGEKYFNILLSRSLFQDIIKDDR 420

Query: 421 GRITHFKMHDLIHDVALAILSTRQKS-----VLDP-THWNGK--TSRKLRTLLYNNQEIH 480
           GRITH KMHDLI+++A  I ++++       +LD  +H N +   ++ LRTL+ N Q +H
Sbjct: 421 GRITHCKMHDLIYEIACTISNSQKLQQEHIDLLDKGSHTNHRINNAQNLRTLICNRQVLH 480

Query: 481 H----KVADCVFLRVLEVNSLHMMNNLPDFIAKLKHLRYLDISSCSMWVMPHSVTTLFNL 540
                K+A+C  LRVL V+S   +  L + I K+KHLRYLDIS+  +  +P+S++ L+NL
Sbjct: 481 KTIFDKIANCTCLRVLVVDS--SITKLSESIGKIKHLRYLDISNSKIEELPNSISLLYNL 540

Query: 541 QTLKLGS-IENLPMNLRNLVRLRHLEFHVYYNTRKMPSHMGELIHLQILSWFVAGFEEGC 600
           QTLKLGS +++LP NL  LV LRHL+F +     + P H+G L  LQ LS F  GFE+G 
Sbjct: 541 QTLKLGSSMKDLPQNLSKLVSLRHLKFSM----PQTPPHLGRLTQLQTLSGFAVGFEKGF 600

Query: 601 KIEELGNLKNLKGQLQLSNLEQVRSKEEALAAKLVNKKNLRELTFEWSIDILRECSSYND 660
           KI ELG LKNLKG+L+LSNL++++ KEEA+++KLV +KNL EL  EW + ILRE ++YND
Sbjct: 601 KIGELGFLKNLKGRLELSNLDRIKHKEEAMSSKLV-EKNLCELFLEWDMHILREGNNYND 660

Query: 661 FEVLEGLQPPKNLSSLKITNFGGKFLPAATFVENLVFLCLYGCTKCERLPMLGQLANLQE 720
           FEVLEGLQP KNL  L I NF G+ LP A FVENL  + L  C +CE LPMLGQL NL+E
Sbjct: 661 FEVLEGLQPHKNLQFLSIINFAGQLLPPAIFVENLAVIHLRHCVRCEILPMLGQLPNLEE 720

Query: 721 LSICFMDSVRSIGSEFYGI---DSNRRGYFPKLKKFDFCWMCNLEQWELEV-ANHESNHF 780
           L+I ++  +RSIG EFYG      + +  FPKLKKF    M NLEQWE  V  + +   F
Sbjct: 721 LNISYLLCLRSIGYEFYGNYYHPYSHKVLFPKLKKFVLSQMPNLEQWEEVVFISKKDAIF 780

Query: 781 GSLQTLKLDRCGKLTKLPN----------------------GLECCKSVHEVIISNCPNL 840
             L+ L +  C  LT +PN                       L+ C S+ ++ I  C  +
Sbjct: 781 PLLEDLNISFCPILTSIPNIFRRPLKKLHIYGCHEVTGLPKDLQLCTSIEDLKIVGCRKM 840

Query: 841 TLNVEEMHNLSVLLIDGLKFLPKGLALHPNLKTIMIKGCIEDYDYSPFLNLPSLTKLYLN 900
           TLNV+ M +LS   ++GL+  P+GLA   NLK + I  C +D D+SP + L SL KL+L 
Sbjct: 841 TLNVQNMDSLSRFSMNGLQKFPQGLANLKNLKEMTIIECSQDCDFSPLMQLSSLVKLHLV 900

Query: 901 DGLGNAT-QLPKQLQHLTALKILAIENFYGIEVLPEWLRKLTCLETLDLVRCKNLKRLPS 920
              G+ T QLP+QL+HL AL+ L I +F GIEVLPEWL  LT LE L L  C NLK+ PS
Sbjct: 901 IFPGSVTEQLPQQLEHLIALRSLYINDFDGIEVLPEWLGNLTSLEVLGLYYCINLKQFPS 925

BLAST of Csa4G016360 vs. TrEMBL
Match: Q2V727_CUCME (R-FOM-2 OS=Cucumis melo PE=3 SV=1)

HSP 1 Score: 649.0 bits (1673), Expect = 8.7e-183
Identity = 385/803 (47.95%), Postives = 509/803 (63.39%), Query Frame = 1

Query: 181  LVIDSSSNEYELPLLIVPILWEELEDCLKEIVGKFGITIIVTTRLDEVANIMGTVSGYRL 240
            LV+D   NE          LW EL+ CL +I G    +I+VTTR  EV  IMGT  G+ L
Sbjct: 275  LVLDDVWNENSF-------LWGELKYCLLKITGNSKNSIVVTTRSAEVTKIMGTCPGHLL 334

Query: 241  EKLPEDHCWSLFKRSANANGVKMTPKLEAIRIKLLQKIDGIPLVAKVLGGAVEFEGDLDR 300
             KL +DHCWSLFK SAN  G+ MT  L  I+ +L++KI G+PLVA+VLG  V+FEGD+++
Sbjct: 335  SKLSDDHCWSLFKESANVYGLSMTSNLGIIQKELVKKIGGVPLVARVLGRTVKFEGDVEK 394

Query: 301  WETTLESIVREIPMKQKSYVLSILQLSVDRLPFVE-KQCFAYCSIFPKDCEVVKENLIRM 360
            WE TL+S++R IP++++ +VLSIL+LSVDRLP    KQCF+YCSIFPKD    K+ LI+M
Sbjct: 395  WEETLKSVLR-IPVQEEDFVLSILKLSVDRLPSSALKQCFSYCSIFPKDFVFEKQELIQM 454

Query: 361  WIAQGFIQPTEGEN-TMEDLGEGHFNFLLSRSLFQDVVKDK----------YG-RITHFK 420
            W+AQGF+QP EG N TME +G+ +F  LLS  LFQD  + +          YG R   +K
Sbjct: 455  WMAQGFLQPQEGRNMTMETVGDIYFKILLSHCLFQDAHETRTEEYKMHDLVYGTRTEEYK 514

Query: 421  MHDLIHDVALAILSTRQKSVLDPTHWNGKTSRK---------LRTLLYNNQEIHH----- 480
            MHDL+HD+A+AI S  Q   L+P++ + K  +K         LRT+ +  +  H+     
Sbjct: 515  MHDLVHDIAMAI-SRDQNLQLNPSNISEKELQKKEIKNVACKLRTIDFIQKIPHNIGQLT 574

Query: 481  ----KVADCVFLRVLEVNSLHMMNNLPDFIAKLKHLRYLDISSCSMWV-MPHSVTTLFNL 540
                K+ + V LR+L+++ +     LP  I +LKHLRYL+I+S S  +  P S+ +L NL
Sbjct: 575  FFDVKIRNFVCLRILKISKMSS-EKLPKSIDQLKHLRYLEIASYSTRLKFPESIVSLHNL 634

Query: 541  QTLKL--GSIENLPMNLRNLVRLRHLEFHVYYNTRKMPSHMGELIHLQILSWFVAGFEEG 600
            QTLK     +E  PMN  NLV LRHL+  ++ N  + P H+ +L  LQ LS FV GFEEG
Sbjct: 635  QTLKFLYSFVEEFPMNFSNLVNLRHLK--LWRNVDQTPPHLSQLTQLQTLSHFVIGFEEG 694

Query: 601  CKIEELGNLKNLKGQLQLSNLEQVRSKEEALAAKLVNKKNLRELTFEWSIDILRECSSYN 660
            CKI ELG LKNL+G   L  LE+V SKEEA  A L  K+NL+EL   WS+   ++  +YN
Sbjct: 695  CKIIELGPLKNLQGSSNLLCLEKVESKEEAKGANLAEKENLKELNLSWSMK-RKDNDNYN 754

Query: 661  DFEVLEGLQPPKNLSSLKITNFGGKFLPAATFVENLVFLCLYGCTKCERLPMLGQLANLQ 720
            D EVLEGLQP +NL  L+I +F  + LP   FVENL+ + LYGC  CE+LPMLGQL NL+
Sbjct: 755  DLEVLEGLQPNQNLQILRIHDFTERRLPNKIFVENLIEIGLYGCDNCEKLPMLGQLNNLK 814

Query: 721  ELSICFMDSVRSIGSEFYGIDSNRRGYFPKLKKFDFCWMCNLEQWELEVANHESNH---F 780
            +L IC  D V+ I ++FYG D N+R +FPKL+KF    M NLEQWE  + N  S++   F
Sbjct: 815  KLEICSFDGVQIIDNKFYGNDPNQRRFFPKLEKFVMQNMINLEQWEEVMTNDASSNVTIF 874

Query: 781  GSLQTLKLDRCGKLTKLPNGLECCKSVHEVIISNCPNLTLNVEEMHNLSVLLIDGLKFLP 840
             +L++L++  C KLTK+PNGL+ C S+  V I  C NL +N+     L  L I  L  LP
Sbjct: 875  PNLKSLEISGCPKLTKIPNGLQFCSSIRRVKIYQCSNLGINMRNKPELWYLHIGPLGKLP 934

Query: 841  KGLALHPNLKTIMIKGCIEDYDYSPFLNLPSLTKLYL-NDGLGN--ATQLPKQLQHLTAL 900
            + L    NL  + I G I++YD+    +LPSL K+ L  D L N   TQ+P+QLQHLT+L
Sbjct: 935  EDLCHLMNLGVMTIVGNIQNYDFGILQHLPSLKKITLVEDELSNNSVTQIPQQLQHLTSL 994

Query: 901  KILAIENFYGIEVLPEWLRKLTCLETLDLVRCKNLKRLPSRGAMRCLTKLKDFKVIACPL 944
            + L+IENF GIE LPEWL  L CL+TL  + C+NLK+LPS  AM  LTKL       CP+
Sbjct: 995  EFLSIENFGGIEALPEWLGNLVCLQTLCFLCCRNLKKLPSTEAMLRLTKLNKLYACECPM 1054

BLAST of Csa4G016360 vs. TAIR10
Match: AT3G14470.1 (AT3G14470.1 NB-ARC domain-containing disease resistance protein)

HSP 1 Score: 254.6 bits (649), Expect = 2.4e-67
Identity = 229/782 (29.28%), Postives = 358/782 (45.78%), Query Frame = 1

Query: 176 LDILRLVIDSSSNEYELP-LLIVPILWEELE---DCLKE--IVGKFGITIIVTTRLDEVA 235
           LD+L++ +        LP LL++  LW E     D L++  I    G  I+VTTR   VA
Sbjct: 261 LDVLQVKLKERLTGTGLPFLLVLDDLWNENFADWDLLRQPFIHAAQGSQILVTTRSQRVA 320

Query: 236 NIMGTVSGYRLEKLPEDHCWSLFKRSANANGVK-MTPKLEAIRIKLLQKIDGIPLVAKVL 295
           +IM  V  + L+ L +  CWSLF ++   N    +  ++  +  +++ K  G+PL  K L
Sbjct: 321 SIMCAVHVHNLQPLSDGDCWSLFMKTVFGNQEPCLNREIGDLAERIVHKCRGLPLAVKTL 380

Query: 296 GGAVEFEGDLDRWETTLESIVREIPMKQKSYVLSILQLSVDRLPFVEKQCFAYCSIFPKD 355
           GG + FEG +  WE  L S + ++P   KS +L +L++S   LP   K+CFAYCSIFPK 
Sbjct: 381 GGVLRFEGKVIEWERVLSSRIWDLPA-DKSNLLPVLRVSYYYLPAHLKRCFAYCSIFPKG 440

Query: 356 CEVVKENLIRMWIAQGFIQPTEGENTMED---------LGEGHFNFLLSRSLFQDVVKD- 415
               K+ ++ +W+A+GF+Q T     +E+                   +R +  D + + 
Sbjct: 441 HAFEKDKVVLLWMAEGFLQQTRSSKNLEELGNEYFSELESRSLLQKTKTRYIMHDFINEL 500

Query: 416 ---KYGRITHFKMHD---LIHDVALAILSTRQKSVLDPTHWNGKTSRK-LRTLL------ 475
                G  +  K  D   L        LS  + +  +P  +      K LRT L      
Sbjct: 501 AQFASGEFSS-KFEDGCKLQVSERTRYLSYLRDNYAEPMEFEALREVKFLRTFLPLSLTN 560

Query: 476 -----YNNQEIHHKVADCVF-LRVLEVNSLHMMNNLPDFIAKLKHLRYLDISSCSMWVMP 535
                  +Q +  K+   +  LRVL ++   +    PDF   + H R+LD+S   +  +P
Sbjct: 561 SSRSCCLDQMVSEKLLPTLTRLRVLSLSHYKIARLPPDFFKNISHARFLDLSRTELEKLP 620

Query: 536 HSVTTLFNLQTLKLG---SIENLPMNLRNLVRLRHLEFHVYYNTRKMPSHMGELIHLQIL 595
            S+  ++NLQTL L    S++ LP ++ NL+ LR+L+  +    R+MP   G L  LQ L
Sbjct: 621 KSLCYMYNLQTLLLSYCSSLKELPTDISNLINLRYLDL-IGTKLRQMPRRFGRLKSLQTL 680

Query: 596 SWFVAGFEEGCKIEELGNLKNLKGQLQLSNLEQVRSKEEALAAKLVNKKNLRELTFEW-- 655
           + F     +G +I ELG L +L G+L++  L++V    +A  A L +KK+LRE+ F W  
Sbjct: 681 TTFFVSASDGSRISELGGLHDLHGKLKIVELQRVVDVADAAEANLNSKKHLREIDFVWRT 740

Query: 656 ----SIDILRECSSYNDFEVLEGLQPPKNLSSLKITNFGGKFLP---AATFVENLVFLCL 715
               S +      + N+ EV E L+P +++  L I  + G+  P   +      +V + L
Sbjct: 741 GSSSSENNTNPHRTQNEAEVFEKLRPHRHIEKLAIERYKGRRFPDWLSDPSFSRIVCIRL 800

Query: 716 YGCTKCERLPMLGQLANLQELSICFMDSVRSIGSEFYGIDSNRRGY----FPKLKKFDFC 775
             C  C  LP LGQL  L+EL I  M  ++SIG +FY  D   R      F  L+   F 
Sbjct: 801 RECQYCTSLPSLGQLPCLKELHISGMVGLQSIGRKFYFSDQQLRDQDQQPFRSLETLRFD 860

Query: 776 WMCNLEQWELEVANHESNHFGSLQTLKLDRCGKLT-KLPNGLECCKSVHEVIISNCPNLT 835
            + + ++W L+V     + F SL+ L + RC +LT  LP  L    S+H   I  C  L 
Sbjct: 861 NLPDWQEW-LDVRVTRGDLFPSLKKLFILRCPELTGTLPTFLPSLISLH---IYKCGLLD 920

Query: 836 LNVEEMHNLSVLLIDGLKFLPKGLALHPNLKTIMIKGCIEDYDYSPFLNLPSLTKLYLND 895
              +  H  S                + NL+T+ IK   +           +L K  LN 
Sbjct: 921 FQPDH-HEYS----------------YRNLQTLSIKSSCD-----------TLVKFPLN- 980

Query: 896 GLGNATQLPKQLQHLTALKILAIENFYGIEVLPEWLRKLTCLETLDLVRCKNLKRLPSRG 905
                        +L  L++    + Y +E+  E LR    L  L +  C+NL+ LP   
Sbjct: 981 ----------HFANLDKLEVDQCTSLYSLELSNEHLRGPNALRNLRINDCQNLQLLPKLN 996

HSP 2 Score: 50.4 bits (119), Expect = 6.9e-06
Identity = 45/174 (25.86%), Postives = 80/174 (45.98%), Query Frame = 1

Query: 32  EDELSNLSKWLLDAGALLRDIDREILRKESVKRWADGLEDIVSEAEDLLDELAYEDLRRK 91
           E+ L  LS  LL   A+L D + + +    V++W + L D+V  AED LD++A E LR  
Sbjct: 36  ENLLERLSTALLTITAVLIDAEEKQITNPVVEKWVNELRDVVYHAEDALDDIATEALRLN 95

Query: 92  VETSSRVCNNFK-------FSSVLNPLVRHDMACKMKKITKMLKQHYRNSAPLGLVGKES 151
           +   S   N  +           L+    H +  +++K+T  L++       LGL     
Sbjct: 96  IGAESSSSNRLRQLRGRMSLGDFLDGNSEH-LETRLEKVTIRLERLASQRNILGL----- 155

Query: 152 MEKEDGGNNLRQIRETTSILN-FDVVGRETEVLDILRLVIDSSSNEYELPLLIV 198
             KE      +Q   TTS+++  +V GR+ +  +I+R +I  +  +  + ++ +
Sbjct: 156 --KELTAMIPKQRLPTTSLVDESEVFGRDDDKDEIMRFLIPENGKDNGITVVAI 201

BLAST of Csa4G016360 vs. TAIR10
Match: AT3G14460.1 (AT3G14460.1 LRR and NB-ARC domains-containing disease resistance protein)

HSP 1 Score: 208.0 bits (528), Expect = 2.6e-53
Identity = 199/690 (28.84%), Postives = 318/690 (46.09%), Query Frame = 1

Query: 164 LNFDVVGRETEVLDILRLVIDSSSNEYELP---------------LLIVPILWEELEDCL 223
           +NF+V    T    +L+ +  S+ N  +LP               LL++   W E +   
Sbjct: 233 INFNVF---TVTKAVLQDITSSAVNTEDLPSLQIQLKKTLSGKRFLLVLDDFWSESDSEW 292

Query: 224 KEIVGKF-----GITIIVTTRLDEVANIMGTVSGYRLEKLPEDHCWSLFKRSA--NANGV 283
           +     F     G  I++TTR + V+ +      Y+++ +  + CW L  R A  N +  
Sbjct: 293 ESFQVAFTDAEEGSKIVLTTRSEIVSTVAKAEKIYQMKLMTNEECWELISRFAFGNISVG 352

Query: 284 KMTPKLEAIRIKLLQKIDGIPLVAKVLGGAVEFEGDLDRWETTLESIVREIPMKQKSYVL 343
            +  +LE I  ++ ++  G+PL A+ +   +  + + D W    ++          + +L
Sbjct: 353 SINQELEGIGKRIAEQCKGLPLAARAIASHLRSKPNPDDWYAVSKNF-----SSYTNSIL 412

Query: 344 SILQLSVDRLPFVEKQCFAYCSIFPKDCEVVKENLIRMWIAQGFIQPTEGENTMEDLGEG 403
            +L+LS D LP   K+CFA CSIFPK     +E L+ +W+A   +        +ED+G  
Sbjct: 413 PVLKLSYDSLPPQLKRCFALCSIFPKGHVFDREELVLLWMAIDLLYQPRSSRRLEDIGND 472

Query: 404 HFNFLLSRSLFQ--DVVKDKYGRITHFKMHDLIHDVALA----------------ILSTR 463
           +   L+++S FQ  D+       +T F MHDL++D+A A                I ST 
Sbjct: 473 YLGDLVAQSFFQRLDIT------MTSFVMHDLMNDLAKAVSGDFCFRLEDDNIPEIPSTT 532

Query: 464 QKSVLDPTHWNGKTSRK-------LRTLLYNNQ-------EIHHKVADCVF-----LRVL 523
           +      +  +   + +       LRT+L  N        ++  KV + +      LR+L
Sbjct: 533 RHFSFSRSQCDASVAFRSICGAEFLRTILPFNSPTSLESLQLTEKVLNPLLNALSGLRIL 592

Query: 524 EVNSLHMMNNLPDFIAKLKHLRYLDISSCSMWVMPHSVTTLFNLQTLKLGS---IENLPM 583
            + S + + NLP  +  LK LRYLD+SS  +  +P  V TL NLQTL L +   + +LP 
Sbjct: 593 SL-SHYQITNLPKSLKGLKLLRYLDLSSTKIKELPEFVCTLCNLQTLLLSNCRDLTSLPK 652

Query: 584 NLRNLVRLRHLEFHVYYNTRKMPSHMGELIHLQILSWFVAGFEEGCKIEELGNLKNLKGQ 643
           ++  L+ LR L+  V     +MP  + +L  LQ LS FV G   G  + EL  L +L+G 
Sbjct: 653 SIAELINLRLLDL-VGTPLVEMPPGIKKLRSLQKLSNFVIGRLSGAGLHELKELSHLRGT 712

Query: 644 LQLSNLEQVRSKEEALAAKLVNKKNLRELTFEWSIDILREC-SSYNDF-----EVLEGLQ 703
           L++S L+ V    EA  A L  K  L  L  +W++        S+N       EVL  L+
Sbjct: 713 LRISELQNVAFASEAKDAGLKRKPFLDGLILKWTVKGSGFVPGSFNALACDQKEVLRMLE 772

Query: 704 PPKNLSSLKITNFGG----KFLPAATFVENLVFLCLYGCTKCERLPMLGQLANLQELSIC 763
           P  +L +  I ++ G    K+L  ++F   +  + L  C  C  LP +GQL +L+ LSI 
Sbjct: 773 PHPHLKTFCIESYQGGAFPKWLGDSSFF-GITSVTLSSCNLCISLPPVGQLPSLKYLSIE 832

Query: 764 FMDSVRSIGSEFYGIDSNRRGY-FPKLKKFDFCWMCNLEQWELEVANHESNHFGSLQTLK 780
             + ++ +G +F+  ++N RG  F  L+   F  M   ++W       E   F  LQ L 
Sbjct: 833 KFNILQKVGLDFFFGENNSRGVPFQSLQILKFYGMPRWDEW--ICPELEDGIFPCLQKLI 892

HSP 2 Score: 56.6 bits (135), Expect = 9.7e-08
Identity = 45/182 (24.73%), Postives = 77/182 (42.31%), Query Frame = 1

Query: 18  VNVAAQKISLVWGLEDE--LSNLSKWLLDAGALLRDIDREILRKESVKRWADGLEDIVSE 77
           +N + + + L  G      L  L   L+ A  +L D D+       VK W  G++D   +
Sbjct: 17  INTSQELVELCKGKSSSALLKRLKVALVTANPVLADADQRAEHVREVKHWLTGIKDAFFQ 76

Query: 78  AEDLLDELAYEDLRRKVETSSRVCNN-FKFSSVLNPLVRHDMACKMKKITKMLKQHYRNS 137
           AED+LDEL  E LRR+V   +      F+        ++  +  KM+K+ ++L+ H ++ 
Sbjct: 77  AEDILDELQTEALRRRVVAEAGGLGGLFQNLMAGREAIQKKIEPKMEKVVRLLEHHVKHI 136

Query: 138 APLGLVGKESMEKEDGGNNLRQIRETTSILNFDVVGRETEVLDILRLVIDSSSNEYELPL 197
             +GL  KE  E  +             +    +VGR  + L ++ L++         P 
Sbjct: 137 EVIGL--KEYSETREPQWRQASRSRPDDLPQGRLVGRVEDKLALVNLLLSDDEISIGKPA 196

HSP 3 Score: 34.7 bits (78), Expect = 3.9e-01
Identity = 25/74 (33.78%), Postives = 39/74 (52.70%), Query Frame = 1

Query: 848 TQLPKQLQHLTALKILAIENFYGIEVLPEWLRKLTCLETLDLVRCKNLKRLPSRGAMRCL 907
           T LPK L+ L  L+ L + +   I+ LPE++  L  L+TL L  C++L  LP   ++  L
Sbjct: 586 TNLPKSLKGLKLLRYLDLSSTK-IKELPEFVCTLCNLQTLLLSNCRDLTSLPK--SIAEL 645

Query: 908 TKLKDFKVIACPLL 922
             L+   ++  PL+
Sbjct: 646 INLRLLDLVGTPLV 656

BLAST of Csa4G016360 vs. TAIR10
Match: AT3G50950.2 (AT3G50950.2 HOPZ-ACTIVATED RESISTANCE 1)

HSP 1 Score: 149.8 bits (377), Expect = 8.4e-36
Identity = 139/480 (28.96%), Postives = 219/480 (45.62%), Query Frame = 1

Query: 216 GITIIVTTRLDEVANIMGTVSG--YRLEKLPEDHCWSLFKRSANA--NGVKMTPKLEAIR 275
           G ++IVTTR + VA  +       +R E L  D+ W LF   A A  +G    P+LE + 
Sbjct: 289 GGSVIVTTRSESVAKRVQARDDKTHRPELLSPDNSWLLFCNVAFAANDGTCERPELEDVG 348

Query: 276 IKLLQKIDGIPLVAKVLGGAVEFEGDL-DRWETTLESIVREIP--MKQKSYVLSILQLSV 335
            +++ K  G+PL  K +GG +  +  +   W    E    E+     +   V+S LQLS 
Sbjct: 349 KEIVTKCKGLPLTIKAVGGLLLCKDHVYHEWRRIAEHFQDELRGNTSETDNVMSSLQLSY 408

Query: 336 DRLPFVEKQCFAYCSIFPKDCEVVKENLIRMWIAQGFIQPTEGENTMEDLGEGHFNFLLS 395
           D LP   K C    S++P+DC + K+ L+  WI +GF+    G +  E  GE  F+ L +
Sbjct: 409 DELPSHLKSCILTLSLYPEDCVIPKQQLVHGWIGEGFVMWRNGRSATES-GEDCFSGLTN 468

Query: 396 RSLFQDVVKDKYGRITHFKMHDLIHDVALAILSTRQKSVLDPTHWNG------------- 455
           R L + V K   G I   K+HD++ D+ + I   ++ S  +P   N              
Sbjct: 469 RCLIEVVDKTYSGTIITCKIHDMVRDLVIDI--AKKDSFSNPEGLNCRHLGISGNFDEKQ 528

Query: 456 -KTSRKLRTLLYN---------NQEIHHKVADCVFLRVLEVNSLHM---MNNLPDFIAKL 515
            K + KLR ++           N ++  K  DC +LRVL+++       ++ + D IA L
Sbjct: 529 IKVNHKLRGVVSTTKTGEVNKLNSDLAKKFTDCKYLRVLDISKSIFDAPLSEILDEIASL 588

Query: 516 KHLRYLDISSCSMWV-MPHSVTTLFNLQTLKLGSIENLPMNLRNLVRLRHLEFHVYYNTR 575
           +HL  L +S+    +  P S+  L NLQ L     +NL      +V  + L      N  
Sbjct: 589 QHLACLSLSNTHPLIQFPRSMEDLHNLQILDASYCQNLKQLQPCIVLFKKLLVLDMTNCG 648

Query: 576 KM---PSHMGELIHLQILSWF-VAGFEEGCKIEELGNLKNLKGQLQLSNLEQVRSKEEAL 635
            +   P  +G L+ L++L  F  A    GCK+ E+ NL NL+ +L LS     + +EE L
Sbjct: 649 SLECFPKGIGSLVKLEVLLGFKPARSNNGCKLSEVKNLTNLR-KLGLSLTRGDQIEEEEL 708

Query: 636 AAKLVNKKNLRELTFEWSIDILRECSSYND--FEVLEGLQPPKNLSSLKITNFGGKFLPA 656
            + L+N   L  ++       +    SY D     ++ L PP  L  L +  + GK  P+
Sbjct: 709 DS-LINLSKLMSIS-------INCYDSYGDDLITKIDALTPPHQLHELSLQFYPGKSSPS 756

BLAST of Csa4G016360 vs. TAIR10
Match: AT1G53350.1 (AT1G53350.1 Disease resistance protein (CC-NBS-LRR class) family)

HSP 1 Score: 142.5 bits (358), Expect = 1.3e-33
Identity = 189/785 (24.08%), Postives = 339/785 (43.18%), Query Frame = 1

Query: 49  LRDIDREILRKESVKRWADGLEDIVSEAEDLLDELAYEDLRRKVETSSRVCNNFKFSSVL 108
           L++  REI +  S    +D L  +    E+L+D L   D  + V  S         +++ 
Sbjct: 144 LQERQREIRQTFSRNSESD-LVGLDQSVEELVDHLVENDSVQVVSVSGM--GGIGKTTLA 203

Query: 109 NPLVRHDMACKMKKITKMLKQHYRNSAPLGLVGKESMEKEDGGNNLRQIRE-TTSILNFD 168
             +  HD+          +++H+   + +  V ++   K+     L+ +R     I+  D
Sbjct: 204 RQVFHHDI----------VRRHFDGFSWV-CVSQQFTRKDVWQRILQDLRPYDEGIIQMD 263

Query: 169 VVGRETEVLDILRLVIDSSSNEYELPLLIVPILWEELE-DCLKEIVG-KFGITIIVTTRL 228
               + E+ ++L       S  Y   LL++  +W+E + D +K +   K G  +++T+R 
Sbjct: 264 EYTLQGELFELLE------SGRY---LLVLDDVWKEEDWDRIKAVFPHKRGWKMLLTSRN 323

Query: 229 DEVA-NIMGTVSGYRLEKLPEDHCWSLFKRSANANGVKMTPKL-EAIRIKLLQKIDGIPL 288
           + +  +   T   +R   L  +  W LF+R  ++   K   K+ EA+  +++    G+PL
Sbjct: 324 EGLGLHADPTCFAFRPRILTPEQSWKLFERIVSSRRDKTEFKVDEAMGKEMVTYCGGLPL 383

Query: 289 VAKVLGGAVEFEGDLDRWETTLESIVREIPMKQ------KSYVLSILQLSVDRLPFVEKQ 348
             KVLGG +  +  +  W+    +IV  I  K        + V  +L LS + LP   K 
Sbjct: 384 AVKVLGGLLAKKHTVLEWKRVHSNIVTHIVGKSGLSDDNSNSVYRVLSLSYEDLPMQLKH 443

Query: 349 CFAYCSIFPKDCEVVKENLIRMWIAQGFIQPTEGENTMEDLGEGHFNFLLSRSLFQDVVK 408
           CF Y + FP+D ++  + L   W+A+G I P    +T++D GE +   L+ R++   VV+
Sbjct: 444 CFFYLAHFPEDYKIDVKILFNYWVAEGIITPFHDGSTIQDTGESYLEELVRRNMV--VVE 503

Query: 409 DKY--GRITHFKMHDLIHDVALA-------ILSTRQKSVLDPTHWNGKTSRKLRTLLYNN 468
           + Y   RI + +MHD++ +V L+       I   +  +    T       R  R +L++ 
Sbjct: 504 ESYLTSRIEYCQMHDMMREVCLSKAKEENFIRVVKVPTTTSTTINAQSPCRSRRLVLHSG 563

Query: 469 QEIH------HKVADCV-------------------FLRVLEVNSLHMM-NNLPDFIAKL 528
             +H      +K A  V                    LRVL+++ +      LP  I  L
Sbjct: 564 NALHMLGHKDNKKARSVLIFGVEEKFWKPRGFQCLPLLRVLDLSYVQFEGGKLPSSIGDL 623

Query: 529 KHLRYLDISSCSMWVMPHSVTTLFNLQTLKLGSIENL----PMNLRNLVRLRHLEFHVYY 588
            HLR+L +    +  +P S+  L  L  L LG  + L    P  L+ +  LR+L      
Sbjct: 624 IHLRFLSLYEAGVSHLPSSLGNLKLLLCLNLGVADRLLVHVPNVLKEMQELRYLRL---- 683

Query: 589 NTRKMPS----HMGELIHLQILSWFVAGFEEGCKIEELGNLKNLKGQLQLSNLEQVRSKE 648
             R MP+     +G+L++L+ L+ F           + G++ +L    +LS L  + S E
Sbjct: 684 -PRSMPAKTKLELGDLVNLESLTNFST---------KHGSVTDLLRMTKLSVLNVIFSGE 743

Query: 649 ---EALAAKLVNKKNLRELTFEWSIDILRECSSYNDFEVLEGLQPPKNLSSLKITNFGGK 708
              E L   L   +NL  L+F      +   +   +  VL+ +    +L  L ++    +
Sbjct: 744 CTFETLLLSLRELRNLETLSFH-DFQKVSVANHGGELLVLDFI----HLKDLTLSMHLPR 803

Query: 709 FLPAATFVENLVFLCLYGCTKCE-RLPMLGQLANLQELSICFMDSVRSIGSEFYGIDSNR 768
           F     F  +L  + L GC   E  +P+L +L +L+ +   ++ S   +G          
Sbjct: 804 FPDQYRFPPHLAHIWLIGCRMEEDPMPILEKLLHLKSV---YLSSGAFLGRRMV----CS 863

Query: 769 RGYFPKLKKFDFCWMCNLEQWELEVANHESNHFGSLQTLKLDRCGKLTKLPNGLECCKSV 776
           +G FP+L      +   L +W +     E      L+TL +D C KL +LP+GL+    +
Sbjct: 864 KGGFPQLLALKMSYKKELVEWRV-----EEGSMPCLRTLTIDNCKKLKQLPDGLKYVTCL 872

HSP 2 Score: 45.1 bits (105), Expect = 2.9e-04
Identity = 44/194 (22.68%), Postives = 92/194 (47.42%), Query Frame = 1

Query: 1   MAEFLWTFAVEETLKRTVNVAAQKISLVWGLEDELSNLSKWLLDAGALLRDIDREILRKE 60
           MAE + +F VE+  +    + +++ + + G+++++  L + L    +LL+D D +    E
Sbjct: 1   MAEAVVSFGVEKLWE----LLSRESARLNGIDEQVDGLKRQLGRLQSLLKDADAKKNETE 60

Query: 61  SVKRWADGLEDIVSEAEDLLDELAYEDLRRKVETSSRVCNNFKFSSVLNPLVRHDMACKM 120
            V+ + + ++DIV +A+D+++     +LR K +   +         V     R   A  +
Sbjct: 61  RVRNFLEDVKDIVYDADDIIESFLLNELRGKEKGIKKQVRTLACFLV----DRRKFASDI 120

Query: 121 KKITKMLKQHYRNSAPLGLVGKESMEKEDGGNNL------RQIRETTS-ILNFDVVGRET 180
           + ITK + +       LG+        + GG +L      R+IR+T S     D+VG + 
Sbjct: 121 EGITKRISEVIVGMQSLGI----QHIADGGGRSLSLQERQREIRQTFSRNSESDLVGLDQ 180

Query: 181 EVLDILRLVIDSSS 188
            V +++  ++++ S
Sbjct: 181 SVEELVDHLVENDS 182

HSP 3 Score: 42.7 bits (99), Expect = 1.4e-03
Identity = 58/226 (25.66%), Postives = 95/226 (42.04%), Query Frame = 1

Query: 715 PKLKKFDFCWMCNLEQWELEVANHESNHFGSLQTLKLDRCGKLTKLPNGLECCKSVHEVI 774
           P   K +   + NLE     + N  + H      L++ +   L  + +G EC      + 
Sbjct: 659 PAKTKLELGDLVNLES----LTNFSTKHGSVTDLLRMTKLSVLNVIFSG-ECTFETLLLS 718

Query: 775 ISNCPNL-TLNVEEMHNLSV-------LLIDGLKFLPKGLALH-----------PNLKTI 834
           +    NL TL+  +   +SV       L++D +      L++H           P+L  I
Sbjct: 719 LRELRNLETLSFHDFQKVSVANHGGELLVLDFIHLKDLTLSMHLPRFPDQYRFPPHLAHI 778

Query: 835 MIKGCIEDYDYSPFLN-LPSLTKLYLNDG--LGNATQLPKQLQHLTALKILAIENFYGIE 894
            + GC  + D  P L  L  L  +YL+ G  LG      K        ++LA++  Y  E
Sbjct: 779 WLIGCRMEEDPMPILEKLLHLKSVYLSSGAFLGRRMVCSKG----GFPQLLALKMSYKKE 838

Query: 895 VLPEWLRK---LTCLETLDLVRCKNLKRLPSRGAMRCLTKLKDFKV 916
           ++ EW  +   + CL TL +  CK LK+LP    ++ +T LK+ K+
Sbjct: 839 LV-EWRVEEGSMPCLRTLTIDNCKKLKQLPD--GLKYVTCLKELKI 872

HSP 4 Score: 29.3 bits (64), Expect = 1.7e+01
Identity = 18/57 (31.58%), Postives = 29/57 (50.88%), Query Frame = 1

Query: 857 LTALKILAIENFYGIEVLPEWLRKLTCLETLDLVRCKN--LKRLPSRGAMR--CLTK 910
           +  L+ L I+N   ++ LP+ L+ +TCL+ L + R K    +RL     ++  CL K
Sbjct: 840 MPCLRTLTIDNCKKLKQLPDGLKYVTCLKELKIERMKREWTERLKDTDGLKHICLEK 896

BLAST of Csa4G016360 vs. TAIR10
Match: AT3G07040.1 (AT3G07040.1 NB-ARC domain-containing disease resistance protein)

HSP 1 Score: 140.2 bits (352), Expect = 6.7e-33
Identity = 107/374 (28.61%), Postives = 178/374 (47.59%), Query Frame = 1

Query: 200 LWEELEDCLKEIVGKFGITIIVTTRLDEVANIMGTVSG--YRLEKLPEDHCWSLFKRSAN 259
           LW E+   L +  G +G  +++TTR   VA+    +    + +E L ED  W LF   A 
Sbjct: 293 LWREISIALPD--GIYGSRVMMTTRDMNVASFPYGIGSTKHEIELLKEDEAWVLFSNKAF 352

Query: 260 ANGVKM--TPKLEAIRIKLLQKIDGIPLVAKVLGGAVEFEGDLDRWETTLESIVREIPMK 319
              ++   T  LE I  KL+++  G+PL    LG  +  +     W+    ++  E+   
Sbjct: 353 PASLEQCRTQNLEPIARKLVERCQGLPLAIASLGSMMSTKKFESEWKKVYSTLNWELNNN 412

Query: 320 QK-SYVLSILQLSVDRLPFVEKQCFAYCSIFPKDCEVVKENLIRMWIAQGFIQPTEGENT 379
            +   V SI+ LS + LP+  K+CF YCS+FP +  + ++ LIRMW+AQ F++P  G   
Sbjct: 413 HELKIVRSIMFLSFNDLPYPLKRCFLYCSLFPVNYRMKRKRLIRMWMAQRFVEPIRGVKA 472

Query: 380 MEDLGEGHFNFLLSRSLFQDVVKDKYGRITHFKMHDLIHDVALAILSTRQKSVLDPTHWN 439
            E++ + + N L+ R++ Q ++ + +GR   FKMHD+I ++AL++    +   +     +
Sbjct: 473 -EEVADSYLNELVYRNMLQVILWNPFGRPKAFKMHDVIWEIALSVSKLERFCDVYNDDSD 532

Query: 440 GKTSRKLRTLLYNNQEIHHKVADCVFLRVLEVNSL-------HMMNNLPDFIAKLKHLRY 499
           G  + +      +      K      +R   ++SL       H M  LP     L  LR 
Sbjct: 533 GDDAAETMENYGSRHLCIQKEMTPDSIRATNLHSLLVCSSAKHKMELLP----SLNLLRA 592

Query: 500 LDISSCSMWVMPHSVTTLFNLQTLKLG--SIENLPMNLRNLVRLRHLEFHVYYNTRKMPS 559
           LD+   S+  +P  + T+FNL+ L L    ++ LP N   LV L  L    +    ++P 
Sbjct: 593 LDLEDSSISKLPDCLVTMFNLKYLNLSKTQVKELPKNFHKLVNLETLNTK-HSKIEELPL 652

BLAST of Csa4G016360 vs. NCBI nr
Match: gi|700197939|gb|KGN53097.1| (hypothetical protein Csa_4G016360 [Cucumis sativus])

HSP 1 Score: 1928.7 bits (4995), Expect = 0.0e+00
Identity = 958/958 (100.00%), Postives = 958/958 (100.00%), Query Frame = 1

Query: 1   MAEFLWTFAVEETLKRTVNVAAQKISLVWGLEDELSNLSKWLLDAGALLRDIDREILRKE 60
           MAEFLWTFAVEETLKRTVNVAAQKISLVWGLEDELSNLSKWLLDAGALLRDIDREILRKE
Sbjct: 1   MAEFLWTFAVEETLKRTVNVAAQKISLVWGLEDELSNLSKWLLDAGALLRDIDREILRKE 60

Query: 61  SVKRWADGLEDIVSEAEDLLDELAYEDLRRKVETSSRVCNNFKFSSVLNPLVRHDMACKM 120
           SVKRWADGLEDIVSEAEDLLDELAYEDLRRKVETSSRVCNNFKFSSVLNPLVRHDMACKM
Sbjct: 61  SVKRWADGLEDIVSEAEDLLDELAYEDLRRKVETSSRVCNNFKFSSVLNPLVRHDMACKM 120

Query: 121 KKITKMLKQHYRNSAPLGLVGKESMEKEDGGNNLRQIRETTSILNFDVVGRETEVLDILR 180
           KKITKMLKQHYRNSAPLGLVGKESMEKEDGGNNLRQIRETTSILNFDVVGRETEVLDILR
Sbjct: 121 KKITKMLKQHYRNSAPLGLVGKESMEKEDGGNNLRQIRETTSILNFDVVGRETEVLDILR 180

Query: 181 LVIDSSSNEYELPLLIVPILWEELEDCLKEIVGKFGITIIVTTRLDEVANIMGTVSGYRL 240
           LVIDSSSNEYELPLLIVPILWEELEDCLKEIVGKFGITIIVTTRLDEVANIMGTVSGYRL
Sbjct: 181 LVIDSSSNEYELPLLIVPILWEELEDCLKEIVGKFGITIIVTTRLDEVANIMGTVSGYRL 240

Query: 241 EKLPEDHCWSLFKRSANANGVKMTPKLEAIRIKLLQKIDGIPLVAKVLGGAVEFEGDLDR 300
           EKLPEDHCWSLFKRSANANGVKMTPKLEAIRIKLLQKIDGIPLVAKVLGGAVEFEGDLDR
Sbjct: 241 EKLPEDHCWSLFKRSANANGVKMTPKLEAIRIKLLQKIDGIPLVAKVLGGAVEFEGDLDR 300

Query: 301 WETTLESIVREIPMKQKSYVLSILQLSVDRLPFVEKQCFAYCSIFPKDCEVVKENLIRMW 360
           WETTLESIVREIPMKQKSYVLSILQLSVDRLPFVEKQCFAYCSIFPKDCEVVKENLIRMW
Sbjct: 301 WETTLESIVREIPMKQKSYVLSILQLSVDRLPFVEKQCFAYCSIFPKDCEVVKENLIRMW 360

Query: 361 IAQGFIQPTEGENTMEDLGEGHFNFLLSRSLFQDVVKDKYGRITHFKMHDLIHDVALAIL 420
           IAQGFIQPTEGENTMEDLGEGHFNFLLSRSLFQDVVKDKYGRITHFKMHDLIHDVALAIL
Sbjct: 361 IAQGFIQPTEGENTMEDLGEGHFNFLLSRSLFQDVVKDKYGRITHFKMHDLIHDVALAIL 420

Query: 421 STRQKSVLDPTHWNGKTSRKLRTLLYNNQEIHHKVADCVFLRVLEVNSLHMMNNLPDFIA 480
           STRQKSVLDPTHWNGKTSRKLRTLLYNNQEIHHKVADCVFLRVLEVNSLHMMNNLPDFIA
Sbjct: 421 STRQKSVLDPTHWNGKTSRKLRTLLYNNQEIHHKVADCVFLRVLEVNSLHMMNNLPDFIA 480

Query: 481 KLKHLRYLDISSCSMWVMPHSVTTLFNLQTLKLGSIENLPMNLRNLVRLRHLEFHVYYNT 540
           KLKHLRYLDISSCSMWVMPHSVTTLFNLQTLKLGSIENLPMNLRNLVRLRHLEFHVYYNT
Sbjct: 481 KLKHLRYLDISSCSMWVMPHSVTTLFNLQTLKLGSIENLPMNLRNLVRLRHLEFHVYYNT 540

Query: 541 RKMPSHMGELIHLQILSWFVAGFEEGCKIEELGNLKNLKGQLQLSNLEQVRSKEEALAAK 600
           RKMPSHMGELIHLQILSWFVAGFEEGCKIEELGNLKNLKGQLQLSNLEQVRSKEEALAAK
Sbjct: 541 RKMPSHMGELIHLQILSWFVAGFEEGCKIEELGNLKNLKGQLQLSNLEQVRSKEEALAAK 600

Query: 601 LVNKKNLRELTFEWSIDILRECSSYNDFEVLEGLQPPKNLSSLKITNFGGKFLPAATFVE 660
           LVNKKNLRELTFEWSIDILRECSSYNDFEVLEGLQPPKNLSSLKITNFGGKFLPAATFVE
Sbjct: 601 LVNKKNLRELTFEWSIDILRECSSYNDFEVLEGLQPPKNLSSLKITNFGGKFLPAATFVE 660

Query: 661 NLVFLCLYGCTKCERLPMLGQLANLQELSICFMDSVRSIGSEFYGIDSNRRGYFPKLKKF 720
           NLVFLCLYGCTKCERLPMLGQLANLQELSICFMDSVRSIGSEFYGIDSNRRGYFPKLKKF
Sbjct: 661 NLVFLCLYGCTKCERLPMLGQLANLQELSICFMDSVRSIGSEFYGIDSNRRGYFPKLKKF 720

Query: 721 DFCWMCNLEQWELEVANHESNHFGSLQTLKLDRCGKLTKLPNGLECCKSVHEVIISNCPN 780
           DFCWMCNLEQWELEVANHESNHFGSLQTLKLDRCGKLTKLPNGLECCKSVHEVIISNCPN
Sbjct: 721 DFCWMCNLEQWELEVANHESNHFGSLQTLKLDRCGKLTKLPNGLECCKSVHEVIISNCPN 780

Query: 781 LTLNVEEMHNLSVLLIDGLKFLPKGLALHPNLKTIMIKGCIEDYDYSPFLNLPSLTKLYL 840
           LTLNVEEMHNLSVLLIDGLKFLPKGLALHPNLKTIMIKGCIEDYDYSPFLNLPSLTKLYL
Sbjct: 781 LTLNVEEMHNLSVLLIDGLKFLPKGLALHPNLKTIMIKGCIEDYDYSPFLNLPSLTKLYL 840

Query: 841 NDGLGNATQLPKQLQHLTALKILAIENFYGIEVLPEWLRKLTCLETLDLVRCKNLKRLPS 900
           NDGLGNATQLPKQLQHLTALKILAIENFYGIEVLPEWLRKLTCLETLDLVRCKNLKRLPS
Sbjct: 841 NDGLGNATQLPKQLQHLTALKILAIENFYGIEVLPEWLRKLTCLETLDLVRCKNLKRLPS 900

Query: 901 RGAMRCLTKLKDFKVIACPLLLLGGQADQEGAKYLHIPAYLCHVYQSRGSPLSKTSSI 959
           RGAMRCLTKLKDFKVIACPLLLLGGQADQEGAKYLHIPAYLCHVYQSRGSPLSKTSSI
Sbjct: 901 RGAMRCLTKLKDFKVIACPLLLLGGQADQEGAKYLHIPAYLCHVYQSRGSPLSKTSSI 958

BLAST of Csa4G016360 vs. NCBI nr
Match: gi|449469162|ref|XP_004152290.1| (PREDICTED: disease resistance protein RGA2-like [Cucumis sativus])

HSP 1 Score: 1542.7 bits (3993), Expect = 0.0e+00
Identity = 759/759 (100.00%), Postives = 759/759 (100.00%), Query Frame = 1

Query: 200  LWEELEDCLKEIVGKFGITIIVTTRLDEVANIMGTVSGYRLEKLPEDHCWSLFKRSANAN 259
            LWEELEDCLKEIVGKFGITIIVTTRLDEVANIMGTVSGYRLEKLPEDHCWSLFKRSANAN
Sbjct: 287  LWEELEDCLKEIVGKFGITIIVTTRLDEVANIMGTVSGYRLEKLPEDHCWSLFKRSANAN 346

Query: 260  GVKMTPKLEAIRIKLLQKIDGIPLVAKVLGGAVEFEGDLDRWETTLESIVREIPMKQKSY 319
            GVKMTPKLEAIRIKLLQKIDGIPLVAKVLGGAVEFEGDLDRWETTLESIVREIPMKQKSY
Sbjct: 347  GVKMTPKLEAIRIKLLQKIDGIPLVAKVLGGAVEFEGDLDRWETTLESIVREIPMKQKSY 406

Query: 320  VLSILQLSVDRLPFVEKQCFAYCSIFPKDCEVVKENLIRMWIAQGFIQPTEGENTMEDLG 379
            VLSILQLSVDRLPFVEKQCFAYCSIFPKDCEVVKENLIRMWIAQGFIQPTEGENTMEDLG
Sbjct: 407  VLSILQLSVDRLPFVEKQCFAYCSIFPKDCEVVKENLIRMWIAQGFIQPTEGENTMEDLG 466

Query: 380  EGHFNFLLSRSLFQDVVKDKYGRITHFKMHDLIHDVALAILSTRQKSVLDPTHWNGKTSR 439
            EGHFNFLLSRSLFQDVVKDKYGRITHFKMHDLIHDVALAILSTRQKSVLDPTHWNGKTSR
Sbjct: 467  EGHFNFLLSRSLFQDVVKDKYGRITHFKMHDLIHDVALAILSTRQKSVLDPTHWNGKTSR 526

Query: 440  KLRTLLYNNQEIHHKVADCVFLRVLEVNSLHMMNNLPDFIAKLKHLRYLDISSCSMWVMP 499
            KLRTLLYNNQEIHHKVADCVFLRVLEVNSLHMMNNLPDFIAKLKHLRYLDISSCSMWVMP
Sbjct: 527  KLRTLLYNNQEIHHKVADCVFLRVLEVNSLHMMNNLPDFIAKLKHLRYLDISSCSMWVMP 586

Query: 500  HSVTTLFNLQTLKLGSIENLPMNLRNLVRLRHLEFHVYYNTRKMPSHMGELIHLQILSWF 559
            HSVTTLFNLQTLKLGSIENLPMNLRNLVRLRHLEFHVYYNTRKMPSHMGELIHLQILSWF
Sbjct: 587  HSVTTLFNLQTLKLGSIENLPMNLRNLVRLRHLEFHVYYNTRKMPSHMGELIHLQILSWF 646

Query: 560  VAGFEEGCKIEELGNLKNLKGQLQLSNLEQVRSKEEALAAKLVNKKNLRELTFEWSIDIL 619
            VAGFEEGCKIEELGNLKNLKGQLQLSNLEQVRSKEEALAAKLVNKKNLRELTFEWSIDIL
Sbjct: 647  VAGFEEGCKIEELGNLKNLKGQLQLSNLEQVRSKEEALAAKLVNKKNLRELTFEWSIDIL 706

Query: 620  RECSSYNDFEVLEGLQPPKNLSSLKITNFGGKFLPAATFVENLVFLCLYGCTKCERLPML 679
            RECSSYNDFEVLEGLQPPKNLSSLKITNFGGKFLPAATFVENLVFLCLYGCTKCERLPML
Sbjct: 707  RECSSYNDFEVLEGLQPPKNLSSLKITNFGGKFLPAATFVENLVFLCLYGCTKCERLPML 766

Query: 680  GQLANLQELSICFMDSVRSIGSEFYGIDSNRRGYFPKLKKFDFCWMCNLEQWELEVANHE 739
            GQLANLQELSICFMDSVRSIGSEFYGIDSNRRGYFPKLKKFDFCWMCNLEQWELEVANHE
Sbjct: 767  GQLANLQELSICFMDSVRSIGSEFYGIDSNRRGYFPKLKKFDFCWMCNLEQWELEVANHE 826

Query: 740  SNHFGSLQTLKLDRCGKLTKLPNGLECCKSVHEVIISNCPNLTLNVEEMHNLSVLLIDGL 799
            SNHFGSLQTLKLDRCGKLTKLPNGLECCKSVHEVIISNCPNLTLNVEEMHNLSVLLIDGL
Sbjct: 827  SNHFGSLQTLKLDRCGKLTKLPNGLECCKSVHEVIISNCPNLTLNVEEMHNLSVLLIDGL 886

Query: 800  KFLPKGLALHPNLKTIMIKGCIEDYDYSPFLNLPSLTKLYLNDGLGNATQLPKQLQHLTA 859
            KFLPKGLALHPNLKTIMIKGCIEDYDYSPFLNLPSLTKLYLNDGLGNATQLPKQLQHLTA
Sbjct: 887  KFLPKGLALHPNLKTIMIKGCIEDYDYSPFLNLPSLTKLYLNDGLGNATQLPKQLQHLTA 946

Query: 860  LKILAIENFYGIEVLPEWLRKLTCLETLDLVRCKNLKRLPSRGAMRCLTKLKDFKVIACP 919
            LKILAIENFYGIEVLPEWLRKLTCLETLDLVRCKNLKRLPSRGAMRCLTKLKDFKVIACP
Sbjct: 947  LKILAIENFYGIEVLPEWLRKLTCLETLDLVRCKNLKRLPSRGAMRCLTKLKDFKVIACP 1006

Query: 920  LLLLGGQADQEGAKYLHIPAYLCHVYQSRGSPLSKTSSI 959
            LLLLGGQADQEGAKYLHIPAYLCHVYQSRGSPLSKTSSI
Sbjct: 1007 LLLLGGQADQEGAKYLHIPAYLCHVYQSRGSPLSKTSSI 1045

BLAST of Csa4G016360 vs. NCBI nr
Match: gi|449469162|ref|XP_004152290.1| (PREDICTED: disease resistance protein RGA2-like [Cucumis sativus])

HSP 1 Score: 391.0 bits (1003), Expect = 6.1e-105
Identity = 199/200 (99.50%), Postives = 200/200 (100.00%), Query Frame = 1

Query: 1   MAEFLWTFAVEETLKRTVNVAAQKISLVWGLEDELSNLSKWLLDAGALLRDIDREILRKE 60
           MAEFLWTFAVEETLKRTVNVAAQKISLVWGLEDELSNLSKWLLDAGALLRDIDREILRKE
Sbjct: 1   MAEFLWTFAVEETLKRTVNVAAQKISLVWGLEDELSNLSKWLLDAGALLRDIDREILRKE 60

Query: 61  SVKRWADGLEDIVSEAEDLLDELAYEDLRRKVETSSRVCNNFKFSSVLNPLVRHDMACKM 120
           SVKRWADGLEDIVSEAEDLLDELAYEDLRRKVETSSRVCNNFKFSSVLNPLVRHDMACKM
Sbjct: 61  SVKRWADGLEDIVSEAEDLLDELAYEDLRRKVETSSRVCNNFKFSSVLNPLVRHDMACKM 120

Query: 121 KKITKMLKQHYRNSAPLGLVGKESMEKEDGGNNLRQIRETTSILNFDVVGRETEVLDILR 180
           KKITKMLKQHYRNSAPLGLVGKESMEKEDGGNNLRQIRETTSILNFDVVGRETEVLDILR
Sbjct: 121 KKITKMLKQHYRNSAPLGLVGKESMEKEDGGNNLRQIRETTSILNFDVVGRETEVLDILR 180

Query: 181 LVIDSSSNEYELPLLIVPIL 201
           LVIDSSSNEYELPLLIVPI+
Sbjct: 181 LVIDSSSNEYELPLLIVPIV 200

HSP 2 Score: 1431.8 bits (3705), Expect = 0.0e+00
Identity = 705/760 (92.76%), Postives = 729/760 (95.92%), Query Frame = 1

Query: 200  LWEELEDCLKEIVGKFGITIIVTTRLDEVANIMGTVSGYRLEKLPEDHCWSLFKRSANAN 259
            LWEELEDCLKEIVGKFGITI+VTTRLDEVANIMGTVSGYRLEKLPEDHCWSLFKRSANAN
Sbjct: 287  LWEELEDCLKEIVGKFGITIMVTTRLDEVANIMGTVSGYRLEKLPEDHCWSLFKRSANAN 346

Query: 260  GVKMTPKLEAIRIKLLQKIDGIPLVAKVLGGAVEFEGDLDRWETTLESIVREIPMKQKSY 319
            G+KMTPKLEAIRIKLLQKIDGIPLVAKVLGGAVEFEGDLDRWETTLES+VREIPMKQKSY
Sbjct: 347  GIKMTPKLEAIRIKLLQKIDGIPLVAKVLGGAVEFEGDLDRWETTLESVVREIPMKQKSY 406

Query: 320  VLSILQLSVDRLPFVEKQCFAYCSIFPKDCEVVKENLIRMWIAQGFIQPTEGENTM-EDL 379
            VLSILQLSVDRLPFVEKQCFAYCSIFPKDCEVVKE+LIRMWIAQGFIQP EGEN M EDL
Sbjct: 407  VLSILQLSVDRLPFVEKQCFAYCSIFPKDCEVVKESLIRMWIAQGFIQPPEGENMMMEDL 466

Query: 380  GEGHFNFLLSRSLFQDVVKDKYGRITHFKMHDLIHDVALAILSTRQKSVLDPTHWNGKTS 439
            GEG+FNFLLSRSLFQDVVKDKYG ITHFKMHDLIHDVALAILS RQK VLDPTHWNGKT+
Sbjct: 467  GEGYFNFLLSRSLFQDVVKDKYGSITHFKMHDLIHDVALAILSNRQKMVLDPTHWNGKTA 526

Query: 440  RKLRTLLYNNQEIHHKVADCVFLRVLEVNSLHMMNNLPDFIAKLKHLRYLDISSCSMWVM 499
            RKLRTL+YNNQEIHHK+ADCVFLRVLEVNSL MMNNLPDFIAKLKHLRYLDISSC MW +
Sbjct: 527  RKLRTLIYNNQEIHHKLADCVFLRVLEVNSLRMMNNLPDFIAKLKHLRYLDISSCPMWAI 586

Query: 500  PHSVTTLFNLQTLKLGSIENLPMNLRNLVRLRHLEFHVYYNTRKMPSHMGELIHLQILSW 559
            PHS+TTLFNLQTLKLGSIENLPMNLRNLVRLRHLEFHVYYNTRKMP HMGELIHLQ LS 
Sbjct: 587  PHSITTLFNLQTLKLGSIENLPMNLRNLVRLRHLEFHVYYNTRKMPPHMGELIHLQTLSG 646

Query: 560  FVAGFEEGCKIEELGNLKNLKGQLQLSNLEQVRSKEEALAAKLVNKKNLRELTFEWSIDI 619
            FVAGFEEGCKIEELGNLKNL+G+LQLSNLEQVRSK+EA+A KLV+KKNLRELTFEWSID+
Sbjct: 647  FVAGFEEGCKIEELGNLKNLRGKLQLSNLEQVRSKQEAIAVKLVDKKNLRELTFEWSIDL 706

Query: 620  LRECSSYNDFEVLEGLQPPKNLSSLKITNFGGKFLPAATFVENLVFLCLYGCTKCERLPM 679
            LRE SSYNDFEVLEGLQPPKNL+SLKITNFGGKFLPA TFVENL FLCLYGCTKCERLPM
Sbjct: 707  LREFSSYNDFEVLEGLQPPKNLTSLKITNFGGKFLPAGTFVENLAFLCLYGCTKCERLPM 766

Query: 680  LGQLANLQELSICFMDSVRSIGSEFYGIDSNRRGYFPKLKKFDFCWMCNLEQWELEVANH 739
            LGQL NLQEL ICF+D V SIG+EFYG DSNRRGYFPKLKKFD  WMCNLEQWELEVANH
Sbjct: 767  LGQLPNLQELGICFLDRVISIGNEFYGNDSNRRGYFPKLKKFDLGWMCNLEQWELEVANH 826

Query: 740  ESNHFGSLQTLKLDRCGKLTKLPNGLECCKSVHEVIISNCPNLTLNVEEMHNLSVLLIDG 799
            ESNHFGSLQTLKLDRCGKLTKLPNGLECCKSV EVIISNCPNLTLNVE+MH+LSVLLIDG
Sbjct: 827  ESNHFGSLQTLKLDRCGKLTKLPNGLECCKSVREVIISNCPNLTLNVEKMHSLSVLLIDG 886

Query: 800  LKFLPKGLALHPNLKTIMIKGCIEDYDYSPFLNLPSLTKLYLNDGLGNATQLPKQLQHLT 859
            LKFLPKGLA  PNLKT+MI GCIEDYDYSPFLNLPSLTKLYLNDGLGNATQLP+QLQHLT
Sbjct: 887  LKFLPKGLAHLPNLKTMMITGCIEDYDYSPFLNLPSLTKLYLNDGLGNATQLPQQLQHLT 946

Query: 860  ALKILAIENFYGIEVLPEWLRKLTCLETLDLVRCKNLKRLPSRGAMRCLTKLKDFKVIAC 919
            ALKILAIENFYGIEVLPEWLRKLTCLETLDLVRCKNLKRLPSR AM CLTKLKDFKV+AC
Sbjct: 947  ALKILAIENFYGIEVLPEWLRKLTCLETLDLVRCKNLKRLPSREAMLCLTKLKDFKVMAC 1006

Query: 920  PLLLLGGQADQEGAKYLHIPAYLCHVYQSRGSPLSKTSSI 959
            PLLLLGGQADQEGAKYLHIPAYLCHVYQSRGS LS TSSI
Sbjct: 1007 PLLLLGGQADQEGAKYLHIPAYLCHVYQSRGSLLSNTSSI 1046

BLAST of Csa4G016360 vs. NCBI nr
Match: gi|659108002|ref|XP_008453965.1| (PREDICTED: disease resistance protein RGA2-like [Cucumis melo])

HSP 1 Score: 372.1 bits (954), Expect = 3.0e-99
Identity = 189/200 (94.50%), Postives = 194/200 (97.00%), Query Frame = 1

Query: 1   MAEFLWTFAVEETLKRTVNVAAQKISLVWGLEDELSNLSKWLLDAGALLRDIDREILRKE 60
           MAEFLWTFAVEETLKRTV VAAQKI++VWGLEDELSNLSKWLLDAGALLRDIDREILRKE
Sbjct: 1   MAEFLWTFAVEETLKRTVKVAAQKIAVVWGLEDELSNLSKWLLDAGALLRDIDREILRKE 60

Query: 61  SVKRWADGLEDIVSEAEDLLDELAYEDLRRKVETSSRVCNNFKFSSVLNPLVRHDMACKM 120
           SVKRW DGLEDIVSEAEDLLDELAYEDLRRKVET+SRVCNNFKFSSVLNPLVRHDMACK+
Sbjct: 61  SVKRWVDGLEDIVSEAEDLLDELAYEDLRRKVETTSRVCNNFKFSSVLNPLVRHDMACKV 120

Query: 121 KKITKMLKQHYRNSAPLGLVGKESMEKEDGGNNLRQIRETTSILNFDVVGRETEVLDILR 180
           K ITKMLKQHYR SAPLGLVGKESMEKEDGGNNLRQIRETTSILNFDVVGRE EVLDILR
Sbjct: 121 KTITKMLKQHYRKSAPLGLVGKESMEKEDGGNNLRQIRETTSILNFDVVGREAEVLDILR 180

Query: 181 LVIDSSSNEYELPLLIVPIL 201
           LVIDSSSNE ELPLLIVPI+
Sbjct: 181 LVIDSSSNESELPLLIVPIV 200

HSP 2 Score: 709.9 bits (1831), Expect = 6.0e-201
Identity = 405/755 (53.64%), Postives = 517/755 (68.48%), Query Frame = 1

Query: 200  LWEELEDCLKEIVGKFGITIIVTTRLDEVANIMGTVSGYRLEKLPEDHCWSLFKRSANAN 259
            LW+EL   LK I GK G +I+VTTR  EVA ++ TV  Y L+KL +DHCW+L K+SANAN
Sbjct: 286  LWDELIGNLKYIAGKSGNSIMVTTRSVEVATMVKTVPIYHLKKLSDDHCWALLKKSANAN 345

Query: 260  GVKMTPKLEAIRIKLLQKIDGIPLVAKVLGGAVEFE-GDLDRWETTLESIVREIPMKQKS 319
             ++M  KLE  +  L++KI G+PL+AKVLGGAV+FE G  + W   +ES  R I ++ K 
Sbjct: 346  QLQMNSKLENTKNILVRKIGGVPLIAKVLGGAVKFEEGGSESWMAKIESFARNISIEDKD 405

Query: 320  YVLSILQLSVDRLPFVE-KQCFAYCSIFPKDCEVVKENLIRMWIAQGFIQPT-EGEN-TM 379
            +VLSIL+LSV+ LP    KQCFAYCS FP+D E  K+  I+MWIA+GFIQP  E EN TM
Sbjct: 406  FVLSILKLSVESLPHSALKQCFAYCSNFPQDYEFDKDEAIQMWIAEGFIQPEQERENLTM 465

Query: 380  EDLGEGHFNFLLSRSLFQDVVKDKYGRITHFKMHDLIHDVALAILSTRQKSVLDPTHWNG 439
            E++GE + NFLLSRSLF+D +K   GRI  FK+HDL+HD+A AI S   K   +P  WNG
Sbjct: 466  ENIGEEYLNFLLSRSLFEDAIKYD-GRIVTFKIHDLMHDIACAI-SNHHKMDSNPISWNG 525

Query: 440  KTSRKLRTLLYNNQEIHHKV-ADCVFLRVLEVNSLHMMNNLPDFIAKLKHLRYLDISSCS 499
            K++RKLRTL+  N+E  HK+  D + LRVL +      N L   + KL HLRYLDIS+C+
Sbjct: 526  KSTRKLRTLICENEEAFHKIQTDIICLRVLVLKWFDT-NTLSTIMDKLIHLRYLDISNCN 585

Query: 500  M-WVMPHSVTTLFNLQTLKLGSIE-NLPMNLRNLVRLRHLEFHVYYNTRKMPSHMGELIH 559
            +  ++  S+  L+NLQTLKLG IE +LP NLRNLV LRHLEF  +++  +MPSHMG +IH
Sbjct: 586  INKLLRDSICALYNLQTLKLGYIECDLPKNLRNLVNLRHLEFKKFFDMGQMPSHMGNMIH 645

Query: 560  LQILSWFVAGFEEGCKIEELGNLKNLKGQLQLSNLEQVRSKEEALAAKLVNKKNLRELTF 619
            LQ LS FV G E+GCKI+ELG LK+LKG L L NL+ V++K+EA+AAKLV KK LR L F
Sbjct: 646  LQTLSEFVVGLEKGCKIDELGPLKDLKGTLTLKNLQNVQNKDEAMAAKLVEKKYLRHLIF 705

Query: 620  EWSIDIL--RECSSYNDFEVLEGLQPPKNLSSLKITNFGGKFLPAATFVENLVFLCLYGC 679
            +W +++    E    ++ +VLEGLQP KN+ SL I  F G+ L    FVENLV + L  C
Sbjct: 706  QWFLNLYDRGEYDEDDNKQVLEGLQPHKNVQSLDIRGFQGRVLNNNIFVENLVEIRLVDC 765

Query: 680  TKCERLPMLGQLANLQELSICFMDSVRSIGSEFYGIDSNRR--GYFPKLKKFDFCWMCNL 739
             +CE LPMLGQL NL++L I  M+SVRSIGSEFYG+D N R    FP+L KF  C +  L
Sbjct: 766  GRCEVLPMLGQLPNLKKLEIISMNSVRSIGSEFYGVDCNDRNSSAFPQLNKFHICGLKKL 825

Query: 740  EQWELEVANHESNHFGSLQTLKLDRCGKLTKLPNGLECCKSVHEVIISNCPNLTLNVEEM 799
            +QW+ E     SN FG L+ L L  C +L KLP+GLE C S+  + I  CPNL LNV+ +
Sbjct: 826  QQWD-EATVFASNRFGCLKELILSGCHQLAKLPSGLEGCYSIEYLAIDGCPNLMLNVQNL 885

Query: 800  HNLSVLLIDGLKFLPKGLALHPNLKTIMIKGCIEDYDYSPFLNLPS-LTKLYLND---GL 859
            +NL  L I GLK LP       NLK + I GC+++Y++SPF++L S L +L L D     
Sbjct: 886  YNLYHLDIRGLKRLPDEFGKLTNLKKLRIGGCMQNYEFSPFIHLSSQLVELELTDDGSSG 945

Query: 860  GNATQLPKQLQHLTALKILAIENFYGIEVLPEWLRKLTCLETLDLVRCKNLKRLPSRGAM 919
               TQLP+QLQHLT LK+L I +F  IEVLPEWL  LTCL TL  + CKNLK LPSR A+
Sbjct: 946  SETTQLPQQLQHLTNLKVLKIADFDDIEVLPEWLGNLTCLATLVFLECKNLKELPSREAI 1005

Query: 920  RCLTKLKDFKVIACPLLLLGGQADQEGAKYLHIPA 940
            + LTKL D  +  CP LLL G+ DQE AK  H+P+
Sbjct: 1006 QRLTKLDDLVIDGCPKLLL-GEGDQERAKLSHLPS 1035

BLAST of Csa4G016360 vs. NCBI nr
Match: gi|449445363|ref|XP_004140442.1| (PREDICTED: disease resistance protein RGA2-like [Cucumis sativus])

HSP 1 Score: 181.8 bits (460), Expect = 5.7e-42
Identity = 103/202 (50.99%), Postives = 135/202 (66.83%), Query Frame = 1

Query: 1   MAEFLWTFAVEETLKRTVNVAAQKISLVWGLEDELSNLSKWLLDAGALLRDIDREILRKE 60
           MAEFLWTFAV+E LK+ VN  A++ISL WGLE ELS+L KWLL A  +L DI+ +     
Sbjct: 1   MAEFLWTFAVQEVLKKIVNFGAEQISLAWGLEKELSHLKKWLLKAQTILADINTKKSHHH 60

Query: 61  SVKRWADGLEDIVSEAEDLLDELAYEDLRRKVETSSRVCNNFKFSSVLNP-----LVRHD 120
           SV  W + L DI+ EA+DLLDE+ YE +R+ VE + ++    K    ++P     L    
Sbjct: 61  SVGLWVEELHDIIYEADDLLDEIVYEQIRQTVEQTGKL---RKVRDSISPSKNSFLFGLK 120

Query: 121 MACKMKKITKMLKQHYRNSAPLGLVGKESMEKEDGGNNLRQIRETTSILNFDVVGRETEV 180
           MA KMKKITK L +HY  ++PLGLVG ES  + +    L QIRETTSIL+F+V GRE EV
Sbjct: 121 MAKKMKKITKTLYEHYCEASPLGLVGDESTTESEAA--LNQIRETTSILDFEVEGREAEV 180

Query: 181 LDILRLVIDSSSNEYELPLLIV 198
           L+IL+LVIDS+  ++   + IV
Sbjct: 181 LEILKLVIDSTDEDHISVISIV 197

HSP 2 Score: 687.2 bits (1772), Expect = 4.1e-194
Identity = 404/767 (52.67%), Postives = 521/767 (67.93%), Query Frame = 1

Query: 200  LWEELEDCLKEIVGKFGITIIVTTRLDEVANIMGTVSGYRLEKLPEDHCWSLFKRSANAN 259
            LW+EL  CLK I GK G TI+VTTR +EVA ++  +S YRL+KL  D CW+LFK SANAN
Sbjct: 287  LWDELRGCLKHIAGKPGNTIMVTTRNEEVATMVEPISIYRLKKLSNDQCWALFKESANAN 346

Query: 260  GVKMTPKLEAIRIKLLQKIDGIPLVAKVLGGAVEF-EGDLDR------WETTLESIVREI 319
             + M  KLE ++ +L++K+ G+PLVAKVLGGAV+F E +L+       W T +ESIVR I
Sbjct: 347  QLPMNSKLEIMKKELVRKMGGVPLVAKVLGGAVKFEETELEEEDHEISWMTKVESIVRNI 406

Query: 320  PMKQKSYVLSILQLSVDRLPF-VEKQCFAYCSIFPKDCEVVKENLIRMWIAQGFIQPTEG 379
             ++ K +VLSIL+LSVD LP  V KQC AYCS F +D +  K++LI+MWIAQGFIQP +G
Sbjct: 407  SLEDKDFVLSILKLSVDSLPNPVLKQCVAYCSNFSQDYDFQKDDLIKMWIAQGFIQPGQG 466

Query: 380  EN---TMEDLGEGHFNFLLSRSLFQDVVKDKYGRITHFKMHDLIHDVALAILSTRQKSVL 439
             +    MED+GE +FNFLLSRS+FQDV +D   RI  FKMHDL+HD+A AI S+ Q    
Sbjct: 467  RDKNLLMEDIGEQYFNFLLSRSIFQDVTRDANKRIVGFKMHDLMHDIACAI-SSHQNVES 526

Query: 440  DPTHWNGKTSRKLRTLLYNNQEIHH-KVADCVFLRVLEVNSLHMMNNLPDFIAKLKHLRY 499
            +P + +GK+ RKLRTL+ N++ I++    D V LRVL+V       +L   I KL HLRY
Sbjct: 527  NPNNLSGKSVRKLRTLICNDEVINYLNQNDIVCLRVLKV-IFQSHTDLWIPIDKLIHLRY 586

Query: 500  LDISSCSM-WVMPHSVTTLFNLQTLKLGSIENLPMNLRNLVRLRHLEFHVYYNTRKMPSH 559
            LDIS CS+  ++  S++ L+NLQTLKLG    LP NLR LV LRHLEF ++ +T  MPS 
Sbjct: 587  LDISECSINKLLLESLSLLYNLQTLKLGQ-SGLPKNLRKLVNLRHLEFKMFGDT-AMPSD 646

Query: 560  MGELIHLQILSWFVAGFEEGCKIEELGNLKNLKGQLQLSNLEQVRSKEEALAAKLVNKKN 619
            MG LIHLQ LS F+ GFE+GCKIEELG LKNLKG+L L+NL +V++K+EA+AAKLV KKN
Sbjct: 647  MGNLIHLQSLSGFLVGFEKGCKIEELGPLKNLKGKLTLTNLWRVQNKDEAMAAKLVEKKN 706

Query: 620  LRELTFEWSIDILRECSSYND--FEVLEGLQPPKNLSSLKITNFGGKFLPAATFVENLVF 679
            LR L   W  +  +      D   +VLEGLQP KNL SL+I  F GK LP   FVENLV 
Sbjct: 707  LRHLNL-WFFETDKRGEDDEDGIVQVLEGLQPHKNLQSLEILGFRGKVLPTGIFVENLVK 766

Query: 680  LCLYGCTKCERLPMLGQLANLQELSICFMDSVRSIGSEFYGIDSNRRG--YFPKLKKFDF 739
            + L    +CE LPMLGQL NL+EL I +M+SVRSIG+EFYG+DS+ +    FP+LKK   
Sbjct: 767  IRLGHFERCEVLPMLGQLPNLKELEIMYMESVRSIGNEFYGVDSSHQNSVAFPQLKKLSI 826

Query: 740  CWMCNLEQWELEVANHESNHFGSLQTLKLDRCGKLTKLPNGLECCKSVHEVIISNCPNLT 799
              M NLEQW+      ESN FG L+ +++ RC  L KLP+GLE C S+  + I  C NL 
Sbjct: 827  YEMMNLEQWDEATVVLESNLFGCLKEVRIRRCNPLAKLPSGLEGCHSLEYLSIRGCFNLM 886

Query: 800  LNVEEMHNLSVLLIDGLKFLPKGLALHPNLKTIMIKGCIEDYDYSPFLNLPS-LTKLYLN 859
            LNV+ +H L  L IDGLK LPKG+     LK + I GC+++Y++S  ++L S L +L L+
Sbjct: 887  LNVQNLHKLYHLEIDGLKRLPKGMDGLTRLKELKIGGCMQNYEFSSVIHLASQLVELELS 946

Query: 860  DGLGNA-TQLPKQLQHLTALKILAIENFYGIEVLPEWLRKLTCLETLDLVRCKNLKRLPS 919
               G+  TQLP+QLQHLT L++L I  F  IE LPEW+  L  L+TL    C  LK LPS
Sbjct: 947  GRYGSVDTQLPQQLQHLTNLQVLKITQFDCIEALPEWIGNLISLKTLKCSYCFKLKELPS 1006

Query: 920  RGAMRCLTKLKDFKVIACPLLLLGGQADQEGAKYLHIPAYLCHVYQS 948
            R A+  LTKL++  +  CP LL+ G+ DQE AK  H+P+   H  +S
Sbjct: 1007 REAILRLTKLENLDIFECPKLLV-GEGDQERAKLSHLPSKCVHKSES 1047