BLAST of CSPI04G03520 vs. Swiss-Prot
Match:
RGA2_SOLBU (Disease resistance protein RGA2 OS=Solanum bulbocastanum GN=RGA2 PE=1 SV=1)
HSP 1 Score: 420.2 bits (1079), Expect = 6.5e-116
Identity = 337/1028 (32.78%), Postives = 511/1028 (49.71%), Query Frame = 1
Query: 24 KISLVWGLEDELSNLSKWLLDAGALLRDIDREILRKESVKRWADGLEDIVSEAEDLLDEL 83
++ L++G +DE LS A+L D + L + ++ W L E +D+LDE
Sbjct: 20 ELVLLFGFQDEFQRLSSMFSTIQAVLEDAQEKQLNNKPLENWLQKLNAATYEVDDILDEY 79
Query: 84 AYEDLRRKVETSSRVCNNFKFSSVLNPLVRHDMACKMKKITKMLKQHYRNSAPLGLVGKE 143
+ R R + + P RH + +M ++ K LK L K
Sbjct: 80 KTKATRFSQSEYGR------YHPKVIPF-RHKVGKRMDQVMKKLKAIAEERKNFHLHEK- 139
Query: 144 SMEKEDGGNNLRQIRETTSILNF-DVVGRETEVLDILRLVIDSSSNEYELPLLIVPIVGM 203
+E++ RET S+L V GR+ E +I++++I++ S+ L +L PI+GM
Sbjct: 140 IVERQ------AVRRETGSVLTEPQVYGRDKEKDEIVKILINNVSDAQHLSVL--PILGM 199
Query: 204 GGVGKTTLAKLVFRHELIKKHFHETIWICVSEHFNIDEILVAILESLTDKVPTKREAVLR 263
GG+GKTTLA++VF + + +HFH IWICVSE F+ ++ AI+ES+ + P E L
Sbjct: 200 GGLGKTTLAQMVFNDQRVTEHFHSKIWICVSEDFDEKRLIKAIVESIEGR-PLLGEMDLA 259
Query: 264 RLQKELLD----KRCFLVLDDVWNESSKLWEELEDCLKEIVGKFGITIIVTTRLDEVADI 323
LQK+L + KR LVLDDVWNE + W L LK VG G +++ TTRL++V I
Sbjct: 260 PLQKKLQELLNGKRYLLVLDDVWNEDQQKWANLRAVLK--VGASGASVLTTTRLEKVGSI 319
Query: 324 MGTVSGYRLEKLPEDHCWSLFKRSANANGVKMTPKLEAIRIKLLQKIDGIPLVAKVLGGA 383
MGT+ Y L L ++ CW LF + A + ++ P L AI ++++K G+PL AK LGG
Sbjct: 320 MGTLQPYELSNLSQEDCWLLFMQRAFGHQEEINPNLVAIGKEIVKKSGGVPLAAKTLGGI 379
Query: 384 VEFEGDLDRWETTLESIVREIPMKQKSYVLSILQLSVDRLPFVEKQCFAYCSIFPKDCEV 443
+ F+ + WE +S + +P + S +L L+LS +LP KQCFAYC++FPKD ++
Sbjct: 380 LCFKREERAWEHVRDSPIWNLPQDESS-ILPALRLSYHQLPLDLKQCFAYCAVFPKDAKM 439
Query: 444 VKENLIRMWIAQGFIQPTEGENTMEDLGEGHFNFLLSRSLFQDV-VKDKYGRITHFKMHD 503
KE LI +W+A GF+ ++G +ED+G+ + L RS FQ++ VKD G+ T+FKMHD
Sbjct: 440 EKEKLISLWMAHGFLL-SKGNMELEDVGDEVWKELYLRSFFQEIEVKD--GK-TYFKMHD 499
Query: 504 LIHDVALAILSTRQKSVLDPTHWNGKTSRKLRTLLYNNQEIHHKVADC---VFLRVLEVN 563
LIHD+A ++ S S + N + + ++ + + + + LRVL +
Sbjct: 500 LIHDLATSLFSANTSSS-NIREINKHSYTHMMSIGFAEVVFFYTLPPLEKFISLRVLNLG 559
Query: 564 SLHMMNNLPDFIAKLKHLRYLDISSCSMWVMPHSVTTLFNLQTLKLGSIENL---PMNLR 623
N LP I L HLRYL++ M +P + L NLQTL L L P
Sbjct: 560 D-STFNKLPSSIGDLVHLRYLNLYGSGMRSLPKQLCKLQNLQTLDLQYCTKLCCLPKETS 619
Query: 624 NLVRLRHLEFHVYYNTRKMPSHMGELIHLQTLSWFVAGFEEGCKIEELGNLKNLKGQLQL 683
L LR+L + MP +G L L+TL FV G ++G ++ ELGNL NL G +++
Sbjct: 620 KLGSLRNLLLDGSQSLTCMPPRIGSLTCLKTLGQFVVGRKKGYQLGELGNL-NLYGSIKI 679
Query: 684 SNLEQVRSKEEALAAKLVNKKNLRELTFEWSIDILRECSSYNDFEVLEGLQPPKNLSSLK 743
S+LE+V++ ++A A L K NL L+ W+ + + +VLE L+P NL+SLK
Sbjct: 680 SHLERVKNDKDAKEANLSAKGNLHSLSMSWN-NFGPHIYESEEVKVLEALKPHSNLTSLK 739
Query: 744 ITNFGGKFLPA---ATFVENLVFLCLYGCTKCERLPMLGQLANLQELSICF----MDSVR 803
I F G LP + ++N+V + + C LP G L L+ L + + ++ V
Sbjct: 740 IYGFRGIHLPEWMNHSVLKNIVSILISNFRNCSCLPPFGDLPCLESLELHWGSADVEYVE 799
Query: 804 SIGSEFY-GIDSNRRGYFPKLKKFDFCWMCNLEQWELEVANHESNHFGSLQTLKLDRCGK 863
+ + + G + R FP L+K D W+ FGSL+ G
Sbjct: 800 EVDIDVHSGFPTRIR--FPSLRKLDI--------WD----------FGSLK-------GL 859
Query: 864 LTKLPNGLECCKSVHEVIISNCPNLTL--NVEEMHNLSVLLIDGLKFLPKGLALH-PNLK 923
L K G E + E+II CP LTL N+ + +L + P+ + + NLK
Sbjct: 860 LKK--EGEEQFPVLEEMIIHECPFLTLSSNLRALTSLRICYNKVATSFPEEMFKNLANLK 919
Query: 924 TIMIKGCIEDYDYSPFLNLPSLTKLYLNDGLGNATQLPKQLQHLTALKILAIENFYGIEV 983
+ I C N +LP L L ALK L I+ +E
Sbjct: 920 YLTISRC------------------------NNLKELPTSLASLNALKSLKIQLCCALES 964
Query: 984 LP-EWLRKLTCLETLDLVRCKNLKRLPSRGAMRCLTKLKDFKVIACPLLLLGGQ--ADQE 1026
LP E L L+ L L + C LK LP ++ LT L K+ CP L+ + ++
Sbjct: 980 LPEEGLEGLSSLTELFVEHCNMLKCLPE--GLQHLTTLTSLKIRGCPQLIKRCEKGIGED 964
BLAST of CSPI04G03520 vs. Swiss-Prot
Match:
RGA3_SOLBU (Putative disease resistance protein RGA3 OS=Solanum bulbocastanum GN=RGA3 PE=2 SV=2)
HSP 1 Score: 410.6 bits (1054), Expect = 5.1e-113
Identity = 306/886 (34.54%), Postives = 463/886 (52.26%), Query Frame = 1
Query: 168 VVGRETEVLDILRLVIDSSSNEYELPLLIVPIVGMGGVGKTTLAKLVFRHELIKKHFHET 227
V GRE E +I++++I++ S E+P+L PI+GMGG+GKTTLA++VF + I +HF+
Sbjct: 151 VYGREKEEDEIVKILINNVSYSEEVPVL--PILGMGGLGKTTLAQMVFNDQRITEHFNLK 210
Query: 228 IWICVSEHFNIDEILVAILESLTDKVPTKREA--VLRRLQKELLDKRCFLVLDDVWNESS 287
IW+CVS+ F+ ++ AI+ES+ K + + ++LQ+ L KR FLVLDDVWNE
Sbjct: 211 IWVCVSDDFDEKRLIKAIVESIEGKSLGDMDLAPLQKKLQELLNGKRYFLVLDDVWNEDQ 270
Query: 288 KLWEELEDCLKEIVGKFGITIIVTTRLDEVADIMGTVSGYRLEKLPEDHCWSLFKRSANA 347
+ W+ L LK +G G +I++TTRL+++ IMGT+ Y+L L ++ CW LFK+ A
Sbjct: 271 EKWDNLRAVLK--IGASGASILITTRLEKIGSIMGTLQLYQLSNLSQEDCWLLFKQRAFC 330
Query: 348 NGVKMTPKLEAIRIKLLQKIDGIPLVAKVLGGAVEFEGDLDRWETTLESIVREIPMKQKS 407
+ + +PKL I ++++K G+PL AK LGG + F+ + WE +S + +P + S
Sbjct: 331 HQTETSPKLMEIGKEIVKKCGGVPLAAKTLGGLLRFKREESEWEHVRDSEIWNLPQDENS 390
Query: 408 YVLSILQLSVDRLPFVEKQCFAYCSIFPKDCEVVKENLIRMWIAQGFIQPTEGENTMEDL 467
VL L+LS LP +QCFAYC++FPKD ++ KE LI +W+A F+ ++G +ED+
Sbjct: 391 -VLPALRLSYHHLPLDLRQCFAYCAVFPKDTKIEKEYLIALWMAHSFLL-SKGNMELEDV 450
Query: 468 GEGHFNFLLSRSLFQDVVKDKYGRITHFKMHDLIHDVALAILST-------RQKSVLDPT 527
G +N L RS FQ+ ++ K G+ T+FKMHDLIHD+A ++ S RQ +V D
Sbjct: 451 GNEVWNELYLRSFFQE-IEVKSGK-TYFKMHDLIHDLATSMFSASASSRSIRQINVKDDE 510
Query: 528 HWNGKTSRKLRTLLYNNQEIHHKVADCVFLRVLEVNSLHMMNN----LPDFIAKLKHLRY 587
+ + E+ + +F R + + L++ N+ LP + L HLRY
Sbjct: 511 DMMFIVTNYKDMMSIGFSEVVSSYSPSLFKRFVSLRVLNLSNSEFEQLPSSVGDLVHLRY 570
Query: 588 LDISSCSMWVMPHSVTTLFNLQTLKL---GSIENLPMNLRNLVRLRHLEFHVYYNTRKMP 647
LD+S + +P + L NLQTL L S+ LP L LR+L + MP
Sbjct: 571 LDLSGNKICSLPKRLCKLQNLQTLDLYNCQSLSCLPKQTSKLCSLRNLVLD-HCPLTSMP 630
Query: 648 SHMGELIHLQTLSWFVAGFEEGCKIEELGNLKNLKGQLQLSNLEQVRSKEEALAAKLVNK 707
+G L L+TL +FV G +G ++ EL NL NL+G + +++LE+V++ EA A L K
Sbjct: 631 PRIGLLTCLKTLGYFVVGERKGYQLGELRNL-NLRGAISITHLERVKNDMEAKEANLSAK 690
Query: 708 KNLRELTFEWSIDILRECSSYNDFEVLEGLQPPKNLSSLKITNFGGKFLP---AATFVEN 767
NL L+ W E + +VLE L+P NL L+I +F G LP + ++N
Sbjct: 691 ANLHSLSMSWDRPNRYES---EEVKVLEALKPHPNLKYLEIIDFCGFCLPDWMNHSVLKN 750
Query: 768 LVFLCLYGCTKCERLPMLGQLANLQELSICFMDSVRSIGSEFYGIDSNRRGYFPKLKKFD 827
+V + + GC C LP G+L L+ L + SV E G + RR FP L+K
Sbjct: 751 VVSILISGCENCSCLPPFGELPCLESLEL-QDGSVEVEYVEDSGFLTRRR--FPSLRKLH 810
Query: 828 FCWMCNLEQWELEVANHESNHFGSLQTLKLDRCGKLTKLPNGLECCKSVHEVIISNCPNL 887
CNL+ LQ +K G E + E+ IS+CP
Sbjct: 811 IGGFCNLK---------------GLQRMK------------GAEQFPVLEEMKISDCPMF 870
Query: 888 TLNVEEMHNLSVLLIDGLKFLPKGLALHPNLKTIMIKGCIEDYDYSP-----FLNLPSLT 947
+ ++ L I G + GL+ NL T+ ++ + F NL +L
Sbjct: 871 VF--PTLSSVKKLEIWG-EADAGGLSSISNLSTLTSLKIFSNHTVTSLLEEMFKNLENL- 930
Query: 948 KLYLN-DGLGNATQLPKQLQHLTALKILAIENFYGIEVLP-EWLRKLTCLETLDLVRCKN 1007
+YL+ L N +LP L L LK L I Y +E LP E L L+ L L + C
Sbjct: 931 -IYLSVSFLENLKELPTSLASLNNLKCLDIRYCYALESLPEEGLEGLSSLTELFVEHCNM 986
Query: 1008 LKRLPSRGAMRCLTKLKDFKVIACPLLLLGGQ--ADQEGAKYLHIP 1026
LK LP ++ LT L K+ CP L+ + ++ K HIP
Sbjct: 991 LKCLPE--GLQHLTTLTSLKIRGCPQLIKRCEKGIGEDWHKISHIP 986
BLAST of CSPI04G03520 vs. Swiss-Prot
Match:
RGA1_SOLBU (Putative disease resistance protein RGA1 OS=Solanum bulbocastanum GN=RGA1 PE=2 SV=2)
HSP 1 Score: 393.3 bits (1009), Expect = 8.4e-108
Identity = 320/991 (32.29%), Postives = 496/991 (50.05%), Query Frame = 1
Query: 24 KISLVWGLEDELSNLSKWLLDAGALLRDIDREILRKESVKRWADGLEDIVSEAEDLLDEL 83
++ L++G +DE LS A+L D + L + ++ W L E +D+LDE
Sbjct: 20 ELVLLFGFQDEFQRLSSMFSTIQAVLEDAQEKQLNDKPLENWLQKLNAATYEVDDILDEY 79
Query: 84 AYEDLRRKVETSSRVCNNFKFSSVLNPLVRHDMACKMKKITKMLKQHYRNSAPLGLVGKE 143
+ R R + + P RH + +M ++ K L L K
Sbjct: 80 KTKATRFLQSEYGR------YHPKVIPF-RHKVGKRMDQVMKKLNAIAEERKKFHLQEK- 139
Query: 144 SMEKEDGGNNLRQIRETTSILNF-DVVGRETEVLDILRLVIDSSSNEYELPLLIVPIVGM 203
+E++ RET S+L V GR+ E +I++++I+++S+ +L +L PI+GM
Sbjct: 140 IIERQAA------TRETGSVLTEPQVYGRDKEKDEIVKILINTASDAQKLSVL--PILGM 199
Query: 204 GGVGKTTLAKLVFRHELIKKHFHETIWICVSEHFNIDEILVAILESLTDKVPTKREA--V 263
GG+GKTTL+++VF + + + F+ IWIC+S+ FN ++ AI+ES+ K + + +
Sbjct: 200 GGLGKTTLSQMVFNDQRVTERFYPKIWICISDDFNEKRLIKAIVESIEGKSLSDMDLAPL 259
Query: 264 LRRLQKELLDKRCFLVLDDVWNESSKLWEELEDCLKEIVGKFGITIIVTTRLDEVADIMG 323
++LQ+ L KR FLVLDDVWNE W L LK VG G ++ TTRL++V IMG
Sbjct: 260 QKKLQELLNGKRYFLVLDDVWNEDQHKWANLRAVLK--VGASGAFVLTTTRLEKVGSIMG 319
Query: 324 TVSGYRLEKLPEDHCWSLFKRSANANGVKMTPKLEAIRIKLLQKIDGIPLVAKVLGGAVE 383
T+ Y L L + CW LF + A + ++ P L AI ++++K G+PL AK LGG +
Sbjct: 320 TLQPYELSNLSPEDCWFLFMQRAFGHQEEINPNLMAIGKEIVKKCGGVPLAAKTLGGILR 379
Query: 384 FEGDLDRWETTLESIVREIPMKQKSYVLSILQLSVDRLPFVEKQCFAYCSIFPKDCEVVK 443
F+ + WE +S + +P + S +L L+LS LP +QCF YC++FPKD ++ K
Sbjct: 380 FKREEREWEHVRDSPIWNLPQDESS-ILPALRLSYHHLPLDLRQCFVYCAVFPKDTKMAK 439
Query: 444 ENLIRMWIAQGFIQPTEGENTMEDLGEGHFNFLLSRSLFQDVVKDKYGRITHFKMHDLIH 503
ENLI W+A GF+ ++G +ED+G +N L RS FQ++ + G+ T+FKMHDLIH
Sbjct: 440 ENLIAFWMAHGFLL-SKGNLELEDVGNEVWNELYLRSFFQEIEVES-GK-TYFKMHDLIH 499
Query: 504 DVALAILSTRQKSVLDPTHWNGKTSRKLRTLLYNNQEIHHK---VADCVFLRVLEVNSLH 563
D+A ++ S S + N + ++ + + + V LRVL + + +
Sbjct: 500 DLATSLFSANTSSS-NIREINANYDGYMMSIGFAEVVSSYSPSLLQKFVSLRVLNLRNSN 559
Query: 564 MMNNLPDFIAKLKHLRYLDIS-SCSMWVMPHSVTTLFNLQTLKL---GSIENLPMNLRNL 623
+ N LP I L HLRYLD+S + + +P + L NLQTL L S+ LP L
Sbjct: 560 L-NQLPSSIGDLVHLRYLDLSGNFRIRNLPKRLCKLQNLQTLDLHYCDSLSCLPKQTSKL 619
Query: 624 VRLRHLEFHVYYNTRKMPSHMGELIHLQTLSWFVAGFEEGCKIEELGNLKNLKGQLQLSN 683
LR+L T P +G L L++LS FV G +G ++ EL NL NL G + ++
Sbjct: 620 GSLRNLLLDGCSLT-STPPRIGLLTCLKSLSCFVIGKRKGHQLGELKNL-NLYGSISITK 679
Query: 684 LEQVRSKEEALAAKLVNKKNLRELTFEWSIDILRECSSYNDFEVLEGLQPPKNLSSLKIT 743
L++V+ +A A L K NL L W +D D EVLE L+P NL L+I
Sbjct: 680 LDRVKKDTDAKEANLSAKANLHSLCLSWDLD----GKHRYDSEVLEALKPHSNLKYLEIN 739
Query: 744 NFGGKFLP---AATFVENLVFLCLYGCTKCERLPMLGQLANLQELSICFMDSVRSIGSEF 803
FGG LP + ++N+V + + GC C LP G+L L+ L + S E
Sbjct: 740 GFGGIRLPDWMNQSVLKNVVSIRIRGCENCSCLPPFGELPCLESLEL----HTGSADVE- 799
Query: 804 YGIDSNRRGYFPKLKK---FDFCWMCNLEQWELEVANHESNHFGSLQTLKLDRCGKLTKL 863
Y D+ G FP L+K +DF + L + E E F L+ + C + +
Sbjct: 800 YVEDNVHPGRFPSLRKLVIWDFSNLKGLLKMEGE------KQFPVLEEMTFYWC-PMFVI 859
Query: 864 PNGLECCKSVHEVI--------ISNCPNLTLNVEEMHNLSVLLIDGLKFLPKGLALHPNL 923
P L K++ ++ ISN LT +++ N+ + F K LA L
Sbjct: 860 PT-LSSVKTLKVIVTDATVLRSISNLRALT-SLDISDNVEATSLPEEMF--KSLANLKYL 919
Query: 924 KTIMIKGCIEDYDYSPFLNLPSLTKLYLNDGLGNATQLPKQ-LQHLTALKILAIENFYGI 983
K + E LN K D L LP++ ++ LT+L L++ N +
Sbjct: 920 KISFFRNLKELPTSLASLNALKSLKFEFCDAL---ESLPEEGVKGLTSLTELSVSNCMML 961
Query: 984 EVLPEWLRKLTCLETLDLVRCKNLKRLPSRG 990
+ LPE L+ LT L TL + +C + + RG
Sbjct: 980 KCLPEGLQHLTALTTLTITQCPIVFKRCERG 961
BLAST of CSPI04G03520 vs. Swiss-Prot
Match:
RGA4_SOLBU (Putative disease resistance protein RGA4 OS=Solanum bulbocastanum GN=RGA4 PE=2 SV=1)
HSP 1 Score: 387.5 bits (994), Expect = 4.6e-106
Identity = 325/1037 (31.34%), Postives = 504/1037 (48.60%), Query Frame = 1
Query: 14 LKRTVKVAAQKISLVWGLEDELSNLSKWLLDAGALLRDIDREILRKESVKRWADGLEDIV 73
L+ K+ L++G E E LS A+L+D + L+ ++++ W L
Sbjct: 10 LENLTSFIGDKLVLIFGFEKECEKLSSVFSTIQAVLQDAQEKQLKDKAIENWLQKLNSAA 69
Query: 74 SEAEDLLDELAYEDLRRKVETSSRVCNNFKFSSVLNPLVRHDMACKMKKITKMLKQHYRN 133
E +D+L E E +R + SR+ F ++N RH + +MK+I + L
Sbjct: 70 YEVDDILGECKNEAIRFE---QSRL--GFYHPGIIN--FRHKIGRRMKEIMEKLDAISEE 129
Query: 134 SAPLGLVGKESMEKEDGGNNLRQIRETTSILNFD-VVGRETEVLDILRLVIDSSSNEYEL 193
+ K + + RET +L V GR+ E +I++++I++ + EL
Sbjct: 130 RRKFHFLEKITERQAAAAT-----RETGFVLTEPKVYGRDKEEDEIVKILINNVNVAEEL 189
Query: 194 PLLIVPIVGMGGVGKTTLAKLVFRHELIKKHFHETIWICVSEHFNIDEILVAILESLTDK 253
P + PI+GMGG+GKTTLA+++F E + KHF+ IW+CVS+ F+ ++ I+ ++
Sbjct: 190 P--VFPIIGMGGLGKTTLAQMIFNDERVTKHFNPKIWVCVSDDFDEKRLIKTIIGNIERS 249
Query: 254 VPTKRE--AVLRRLQKELLDKRCFLVLDDVWNESSKLWEELEDCLKEIVGKFGITIIVTT 313
P + + ++LQ+ L KR LVLDDVWN+ + W +L L VG G +I+ TT
Sbjct: 250 SPHVEDLASFQKKLQELLNGKRYLLVLDDVWNDDLEKWAKLRAVLT--VGARGASILATT 309
Query: 314 RLDEVADIMGTVSGYRLEKLPEDHCWSLFKRSANANGVKMTPKLEAIRIKLLQKIDGIPL 373
RL++V IMGT+ Y L L LF + A + P L AI ++++K G+PL
Sbjct: 310 RLEKVGSIMGTLQPYHLSNLSPHDSLLLFMQRAFGQQKEANPNLVAIGKEIVKKCGGVPL 369
Query: 374 VAKVLGGAVEFEGDLDRWETTLESIVREIPMKQKSYVLSILQLSVDRLPFVEKQCFAYCS 433
AK LGG + F+ + WE ++ + +P + S +L L+LS LP +QCFAYC+
Sbjct: 370 AAKTLGGLLRFKREESEWEHVRDNEIWSLPQDESS-ILPALRLSYHHLPLDLRQCFAYCA 429
Query: 434 IFPKDCEVVKENLIRMWIAQGFIQPTEGENTMEDLGEGHFNFLLSRSLFQDVVKDKYGRI 493
+FPKD +++KENLI +W+A GF+ ++G +ED+G +N L RS FQ+ ++ K G
Sbjct: 430 VFPKDTKMIKENLITLWMAHGFLL-SKGNLELEDVGNEVWNELYLRSFFQE-IEAKSGN- 489
Query: 494 THFKMHDLIHDVALAILST-------RQKSVLDPTHWNGKTSRKLRTLLYNNQEIHHKVA 553
T+FK+HDLIHD+A ++ S R+ +V D H + + + +
Sbjct: 490 TYFKIHDLIHDLATSLFSASASCGNIREINVKDYKH-----TVSIGFAAVVSSYSPSLLK 549
Query: 554 DCVFLRVLEVNSLHMMNNLPDFIAKLKHLRYLDISSCSMWVMPHSVTTLFNLQTLKLG-- 613
V LRVL + S + LP I L HLRYLD+S + +P + L NLQTL +
Sbjct: 550 KFVSLRVLNL-SYSKLEQLPSSIGDLLHLRYLDLSCNNFRSLPERLCKLQNLQTLDVHNC 609
Query: 614 -SIENLPMNLRNLVRLRHLEFHVYYNTRKMPSHMGELIHLQTLSWFVAGFEEGCKIEELG 673
S+ LP L LRHL T P +G L L+TL +F+ G ++G ++ EL
Sbjct: 610 YSLNCLPKQTSKLSSLRHLVVDGCPLT-STPPRIGLLTCLKTLGFFIVGSKKGYQLGELK 669
Query: 674 NLKNLKGQLQLSNLEQVRSKEEALAAKLVNKKNLRELTFEWSIDILRECSSYNDFEVLEG 733
NL NL G + +++LE+V++ +A A L K NL+ L+ W D S + +VLE
Sbjct: 670 NL-NLCGSISITHLERVKNDTDA-EANLSAKANLQSLSMSWDNDGPNRYES-KEVKVLEA 729
Query: 734 LQPPKNLSSLKITNFGGKFLPA---ATFVENLVFLCLYGCTKCERLPMLGQLANLQELSI 793
L+P NL L+I FGG P+ + +E ++ + + C C LP G+L L+ L +
Sbjct: 730 LKPHPNLKYLEIIAFGGFRFPSWINHSVLEKVISVRIKSCKNCLCLPPFGELPCLENLEL 789
Query: 794 CFMDS-VRSIGSEFYGIDSNRRGYFPKLKKFDFCWMCNLEQWELEVANHESNHFGSLQTL 853
+ V + + + R FP LKK L W F SL+ L
Sbjct: 790 QNGSAEVEYVEEDDVHSRFSTRRSFPSLKK--------LRIW----------FFRSLKGL 849
Query: 854 KLDRCGKLTKLPNGLECCKSVHEVIISNCPNLTLNVEEMHNLSVLLIDGLKFLPKGLALH 913
+ G E + E+ I CP + ++ L + G +GL+
Sbjct: 850 MKE---------EGEEKFPMLEEMAILYCPLFVF--PTLSSVKKLEVHG-NTNTRGLSSI 909
Query: 914 PNLKTIMIKGCIEDYDYSP-----FLNLPSLTKLYLNDGLGNATQLPKQLQHLTALKILA 973
NL T+ +Y + F +L +L L D N LP L L ALK L
Sbjct: 910 SNLSTLTSLRIGANYRATSLPEEMFTSLTNLEFLSFFD-FKNLKDLPTSLTSLNALKRLQ 969
Query: 974 IENFYGIEVLPE-WLRKLTCLETLDLVRCKNLKRLPSRGAMRCLTKLKDFKVIACPLL-- 1026
IE+ +E PE L LT L L + CK LK LP ++ LT L + V CP +
Sbjct: 970 IESCDSLESFPEQGLEGLTSLTQLFVKYCKMLKCLPE--GLQHLTALTNLGVSGCPEVEK 983
BLAST of CSPI04G03520 vs. Swiss-Prot
Match:
R13L1_ARATH (Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana GN=RPPL1 PE=3 SV=1)
HSP 1 Score: 348.6 bits (893), Expect = 2.4e-94
Identity = 299/1019 (29.34%), Postives = 480/1019 (47.11%), Query Frame = 1
Query: 32 EDELSNLSKWLLDAGALLRDIDREILRKESVKRWADGLEDIVSEAEDLLDELAYEDLRRK 91
E+ L LS LL A+L D + + + V++W + L D+V AED LD++A E LR
Sbjct: 36 ENLLERLSTALLTITAVLIDAEEKQITNPVVEKWVNELRDVVYHAEDALDDIATEALRLN 95
Query: 92 VETSSRVCNNFK-------FSSVLNPLVRHDMACKMKKITKMLKQHYRNSAPLGLVGKES 151
+ S N + L+ H + +++K+T L++ LGL
Sbjct: 96 IGAESSSSNRLRQLRGRMSLGDFLDGNSEH-LETRLEKVTIRLERLASQRNILGL----- 155
Query: 152 MEKEDGGNNLRQIRETTSILN-FDVVGRETEVLDILRLVIDSSSNEYELPLLIVPIVGMG 211
KE +Q TTS+++ +V GR+ + +I+R +I + + + +V IVG+G
Sbjct: 156 --KELTAMIPKQRLPTTSLVDESEVFGRDDDKDEIMRFLIPENGKDNGIT--VVAIVGIG 215
Query: 212 GVGKTTLAKLVFRHELIKKHFHETIWICVSEHFNIDEILVAILESLTDKVP--TKREAVL 271
GVGKTTL++L++ + ++ +F +W VSE F++ +I + ES+T + T + +
Sbjct: 216 GVGKTTLSQLLYNDQHVRSYFGTKVWAHVSEEFDVFKITKKVYESVTSRPCEFTDLDVLQ 275
Query: 272 RRLQKELLDKRC--FLVLDDVWNESSKLWEELEDCLKEIVGKFGITIIVTTRLDEVADIM 331
+L++ L LVLDD+WNE+ W+ L I G I+VTTR VA IM
Sbjct: 276 VKLKERLTGTGLPFLLVLDDLWNENFADWDLLRQPF--IHAAQGSQILVTTRSQRVASIM 335
Query: 332 GTVSGYRLEKLPEDHCWSLFKRSANANGVK-MTPKLEAIRIKLLQKIDGIPLVAKVLGGA 391
V + L+ L + CWSLF ++ N + ++ + +++ K G+PL K LGG
Sbjct: 336 CAVHVHNLQPLSDGDCWSLFMKTVFGNQEPCLNREIGDLAERIVHKCRGLPLAVKTLGGV 395
Query: 392 VEFEGDLDRWETTLESIVREIPMKQKSYVLSILQLSVDRLPFVEKQCFAYCSIFPKDCEV 451
+ FEG + WE L S + ++P KS +L +L++S LP K+CFAYCSIFPK
Sbjct: 396 LRFEGKVIEWERVLSSRIWDLPA-DKSNLLPVLRVSYYYLPAHLKRCFAYCSIFPKGHAF 455
Query: 452 VKENLIRMWIAQGFIQPTEGENTMED---------LGEGHFNFLLSRSLFQDVVKD---- 511
K+ ++ +W+A+GF+Q T +E+ +R + D + +
Sbjct: 456 EKDKVVLLWMAEGFLQQTRSSKNLEELGNEYFSELESRSLLQKTKTRYIMHDFINELAQF 515
Query: 512 KYGRITHFKMHD---LIHDVALAILSTRQKSVLDPTHWNGKTSRK-LRTLL--------- 571
G + K D L LS + + +P + K LRT L
Sbjct: 516 ASGEFSS-KFEDGCKLQVSERTRYLSYLRDNYAEPMEFEALREVKFLRTFLPLSLTNSSR 575
Query: 572 --YNNQEIHHKVADCVF-LRVLEVNSLHMMNNLPDFIAKLKHLRYLDISSCSMWVMPHSV 631
+Q + K+ + LRVL ++ + PDF + H R+LD+S + +P S+
Sbjct: 576 SCCLDQMVSEKLLPTLTRLRVLSLSHYKIARLPPDFFKNISHARFLDLSRTELEKLPKSL 635
Query: 632 TTLFNLQTLKLG---SIENLPMNLRNLVRLRHLEFHVYYNTRKMPSHMGELIHLQTLSWF 691
++NLQTL L S++ LP ++ NL+ LR+L+ + R+MP G L LQTL+ F
Sbjct: 636 CYMYNLQTLLLSYCSSLKELPTDISNLINLRYLDL-IGTKLRQMPRRFGRLKSLQTLTTF 695
Query: 692 VAGFEEGCKIEELGNLKNLKGQLQLSNLEQVRSKEEALAAKLVNKKNLRELTFEW----- 751
+G +I ELG L +L G+L++ L++V +A A L +KK+LRE+ F W
Sbjct: 696 FVSASDGSRISELGGLHDLHGKLKIVELQRVVDVADAAEANLNSKKHLREIDFVWRTGSS 755
Query: 752 -SIDILRECSSYNDFEVLEGLQPPKNLSSLKITNFGGKFLP---AATFVENLVFLCLYGC 811
S + + N+ EV E L+P +++ L I + G+ P + +V + L C
Sbjct: 756 SSENNTNPHRTQNEAEVFEKLRPHRHIEKLAIERYKGRRFPDWLSDPSFSRIVCIRLREC 815
Query: 812 TKCERLPMLGQLANLQELSICFMDSVRSIGSEFYGIDSNRRGY----FPKLKKFDFCWMC 871
C LP LGQL L+EL I M ++SIG +FY D R F L+ F +
Sbjct: 816 QYCTSLPSLGQLPCLKELHISGMVGLQSIGRKFYFSDQQLRDQDQQPFRSLETLRFDNLP 875
Query: 872 NLEQWELEVANHESNHFGSLQTLKLDRCGKLT-KLPNGLECCKSVHEVIISNCPNLTLNV 931
+ ++W L+V + F SL+ L + RC +LT LP L S+H I C L
Sbjct: 876 DWQEW-LDVRVTRGDLFPSLKKLFILRCPELTGTLPTFLPSLISLH---IYKCGLLDFQP 935
Query: 932 EEMHNLSVLLIDGLKFLPKGLALHPNLKTIMIKGCIEDYDYSPFLNLPSLTKLYLNDGLG 991
+ H S + NL+T+ IK + +L K LN
Sbjct: 936 DH-HEYS----------------YRNLQTLSIKSSCD-----------TLVKFPLN---- 995
BLAST of CSPI04G03520 vs. TrEMBL
Match:
A0A0A0KXG0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G016360 PE=3 SV=1)
HSP 1 Score: 1549.3 bits (4010), Expect = 0.0e+00
Identity = 757/759 (99.74%), Postives = 758/759 (99.87%), Query Frame = 1
Query: 287 LWEELEDCLKEIVGKFGITIIVTTRLDEVADIMGTVSGYRLEKLPEDHCWSLFKRSANAN 346
LWEELEDCLKEIVGKFGITIIVTTRLDEVA+IMGTVSGYRLEKLPEDHCWSLFKRSANAN
Sbjct: 200 LWEELEDCLKEIVGKFGITIIVTTRLDEVANIMGTVSGYRLEKLPEDHCWSLFKRSANAN 259
Query: 347 GVKMTPKLEAIRIKLLQKIDGIPLVAKVLGGAVEFEGDLDRWETTLESIVREIPMKQKSY 406
GVKMTPKLEAIRIKLLQKIDGIPLVAKVLGGAVEFEGDLDRWETTLESIVREIPMKQKSY
Sbjct: 260 GVKMTPKLEAIRIKLLQKIDGIPLVAKVLGGAVEFEGDLDRWETTLESIVREIPMKQKSY 319
Query: 407 VLSILQLSVDRLPFVEKQCFAYCSIFPKDCEVVKENLIRMWIAQGFIQPTEGENTMEDLG 466
VLSILQLSVDRLPFVEKQCFAYCSIFPKDCEVVKENLIRMWIAQGFIQPTEGENTMEDLG
Sbjct: 320 VLSILQLSVDRLPFVEKQCFAYCSIFPKDCEVVKENLIRMWIAQGFIQPTEGENTMEDLG 379
Query: 467 EGHFNFLLSRSLFQDVVKDKYGRITHFKMHDLIHDVALAILSTRQKSVLDPTHWNGKTSR 526
EGHFNFLLSRSLFQDVVKDKYGRITHFKMHDLIHDVALAILSTRQKSVLDPTHWNGKTSR
Sbjct: 380 EGHFNFLLSRSLFQDVVKDKYGRITHFKMHDLIHDVALAILSTRQKSVLDPTHWNGKTSR 439
Query: 527 KLRTLLYNNQEIHHKVADCVFLRVLEVNSLHMMNNLPDFIAKLKHLRYLDISSCSMWVMP 586
KLRTLLYNNQEIHHKVADCVFLRVLEVNSLHMMNNLPDFIAKLKHLRYLDISSCSMWVMP
Sbjct: 440 KLRTLLYNNQEIHHKVADCVFLRVLEVNSLHMMNNLPDFIAKLKHLRYLDISSCSMWVMP 499
Query: 587 HSVTTLFNLQTLKLGSIENLPMNLRNLVRLRHLEFHVYYNTRKMPSHMGELIHLQTLSWF 646
HSVTTLFNLQTLKLGSIENLPMNLRNLVRLRHLEFHVYYNTRKMPSHMGELIHLQ LSWF
Sbjct: 500 HSVTTLFNLQTLKLGSIENLPMNLRNLVRLRHLEFHVYYNTRKMPSHMGELIHLQILSWF 559
Query: 647 VAGFEEGCKIEELGNLKNLKGQLQLSNLEQVRSKEEALAAKLVNKKNLRELTFEWSIDIL 706
VAGFEEGCKIEELGNLKNLKGQLQLSNLEQVRSKEEALAAKLVNKKNLRELTFEWSIDIL
Sbjct: 560 VAGFEEGCKIEELGNLKNLKGQLQLSNLEQVRSKEEALAAKLVNKKNLRELTFEWSIDIL 619
Query: 707 RECSSYNDFEVLEGLQPPKNLSSLKITNFGGKFLPAATFVENLVFLCLYGCTKCERLPML 766
RECSSYNDFEVLEGLQPPKNLSSLKITNFGGKFLPAATFVENLVFLCLYGCTKCERLPML
Sbjct: 620 RECSSYNDFEVLEGLQPPKNLSSLKITNFGGKFLPAATFVENLVFLCLYGCTKCERLPML 679
Query: 767 GQLANLQELSICFMDSVRSIGSEFYGIDSNRRGYFPKLKKFDFCWMCNLEQWELEVANHE 826
GQLANLQELSICFMDSVRSIGSEFYGIDSNRRGYFPKLKKFDFCWMCNLEQWELEVANHE
Sbjct: 680 GQLANLQELSICFMDSVRSIGSEFYGIDSNRRGYFPKLKKFDFCWMCNLEQWELEVANHE 739
Query: 827 SNHFGSLQTLKLDRCGKLTKLPNGLECCKSVHEVIISNCPNLTLNVEEMHNLSVLLIDGL 886
SNHFGSLQTLKLDRCGKLTKLPNGLECCKSVHEVIISNCPNLTLNVEEMHNLSVLLIDGL
Sbjct: 740 SNHFGSLQTLKLDRCGKLTKLPNGLECCKSVHEVIISNCPNLTLNVEEMHNLSVLLIDGL 799
Query: 887 KFLPKGLALHPNLKTIMIKGCIEDYDYSPFLNLPSLTKLYLNDGLGNATQLPKQLQHLTA 946
KFLPKGLALHPNLKTIMIKGCIEDYDYSPFLNLPSLTKLYLNDGLGNATQLPKQLQHLTA
Sbjct: 800 KFLPKGLALHPNLKTIMIKGCIEDYDYSPFLNLPSLTKLYLNDGLGNATQLPKQLQHLTA 859
Query: 947 LKILAIENFYGIEVLPEWLRKLTCLETLDLVRCKNLKRLPSRGAMRCLTKLKDFKVIACP 1006
LKILAIENFYGIEVLPEWLRKLTCLETLDLVRCKNLKRLPSRGAMRCLTKLKDFKVIACP
Sbjct: 860 LKILAIENFYGIEVLPEWLRKLTCLETLDLVRCKNLKRLPSRGAMRCLTKLKDFKVIACP 919
Query: 1007 LLLLGGQADQEGAKYLHIPAYLCHVYQSRGSPLSKTSSI 1046
LLLLGGQADQEGAKYLHIPAYLCHVYQSRGSPLSKTSSI
Sbjct: 920 LLLLGGQADQEGAKYLHIPAYLCHVYQSRGSPLSKTSSI 958
BLAST of CSPI04G03520 vs. TrEMBL
Match:
A0A0A0KST5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G266890 PE=3 SV=1)
HSP 1 Score: 980.7 bits (2534), Expect = 1.4e-282
Identity = 554/1048 (52.86%), Postives = 720/1048 (68.70%), Query Frame = 1
Query: 1 MAEFLWTFAVEETLKRTVKVAAQKISLVWGLEDELSNLSKWLLDAGALLRDIDREILRKE 60
MAEFLWTFAV+E LK+ V A++ISL WGLE ELS+L KWLL A +L DI+ +
Sbjct: 1 MAEFLWTFAVQEVLKKIVNFGAEQISLAWGLEKELSHLKKWLLKAQTILADINTKKSHHH 60
Query: 61 SVKRWADGLEDIVSEAEDLLDELAYEDLRRKVETSSRVCNNFKFSSVLNP-----LVRHD 120
SV W + L DI+ EA+DLLDE+ YE +R+ VE + ++ K ++P L
Sbjct: 61 SVGLWVEELHDIIYEADDLLDEIVYEQIRQTVEQTGKLR---KVRDSISPSKNSFLFGLK 120
Query: 121 MACKMKKITKMLKQHYRNSAPLGLVGKESMEKEDGGNNLRQIRETTSILNFDVVGRETEV 180
MA KMKKITK L +HY ++PLGLVG ES + + L QIRETTSIL+F+V GRE EV
Sbjct: 121 MAKKMKKITKTLYEHYCEASPLGLVGDESTTESEAA--LNQIRETTSILDFEVEGREAEV 180
Query: 181 LDILRLVIDSSSNEYELPLLIVPIVGMGGVGKTTLAKLVFRHELIKKHFHETIWICVSEH 240
L+IL+LVIDS+ ++ + ++ IVGMGG+GKTTLAK+VF H+ IK HF +T+W+CVS+
Sbjct: 181 LEILKLVIDSTDEDH---ISVISIVGMGGLGKTTLAKMVFNHDAIKGHFDKTVWVCVSKP 240
Query: 241 FNIDEILVAILESLTDKVP--TKREAVLRRLQKELLDKRCFLVLDDVWNESSKLWEELED 300
F + +IL AI + LT+ REA+L RL++E+ K+ FLVLDDVW++ + LW+EL
Sbjct: 241 FIVMKILEAIFQGLTNTSSGLNSREALLNRLREEMQGKKYFLVLDDVWDKENCLWDELIG 300
Query: 301 CLKEIVGKFGITIIVTTRLDEVADIMGTVSGYRLEKLPEDHCWSLFKRSANANGVKMTPK 360
LK I GK G +I+VTTR EVA ++ TV Y L+KL +DHCW+L K+SANAN ++M K
Sbjct: 301 NLKYIAGKSGNSIMVTTRSVEVATMVKTVPIYHLKKLSDDHCWALLKKSANANQLQMNSK 360
Query: 361 LEAIRIKLLQKIDGIPLVAKVLGGAVEFE-GDLDRWETTLESIVREIPMKQKSYVLSILQ 420
LE + L++KI G+PL+AKVLGGAV+FE G + W +ES R I ++ K +VLSIL+
Sbjct: 361 LENTKNILVRKIGGVPLIAKVLGGAVKFEEGGSESWMAKIESFARNISIEDKDFVLSILK 420
Query: 421 LSVDRLPFVE-KQCFAYCSIFPKDCEVVKENLIRMWIAQGFIQPT-EGEN-TMEDLGEGH 480
LSV+ LP KQCFAYCS FP+D E K+ I+MWIA+GFIQP E EN TME++GE +
Sbjct: 421 LSVESLPHSALKQCFAYCSNFPQDYEFDKDEAIQMWIAEGFIQPEQERENLTMENIGEEY 480
Query: 481 FNFLLSRSLFQDVVKDKYGRITHFKMHDLIHDVALAILSTRQKSVLDPTHWNGKTSRKLR 540
NFLLSRSLF+D +K GRI FK+HDL+HD+A AI S K +P WNGK++RKLR
Sbjct: 481 LNFLLSRSLFEDAIKYD-GRIVTFKIHDLMHDIACAI-SNHHKMDSNPISWNGKSTRKLR 540
Query: 541 TLLYNNQEIHHKV-ADCVFLRVLEVNSLHMMNNLPDFIAKLKHLRYLDISSCSM-WVMPH 600
TL+ N+E HK+ D + LRVL + N L + KL HLRYLDIS+C++ ++
Sbjct: 541 TLICENEEAFHKIQTDIICLRVLVLKWFDT-NTLSTIMDKLIHLRYLDISNCNINKLLRD 600
Query: 601 SVTTLFNLQTLKLGSIE-NLPMNLRNLVRLRHLEFHVYYNTRKMPSHMGELIHLQTLSWF 660
S+ L+NLQTLKLG IE +LP NLRNLV LRHLEF +++ +MPSHMG +IHLQTLS F
Sbjct: 601 SICALYNLQTLKLGYIECDLPKNLRNLVNLRHLEFKKFFDMGQMPSHMGNMIHLQTLSEF 660
Query: 661 VAGFEEGCKIEELGNLKNLKGQLQLSNLEQVRSKEEALAAKLVNKKNLRELTFEWSIDIL 720
V G E+GCKI+ELG LK+LKG L L NL+ V++K+EA+AAKLV KK LR L F+W +++
Sbjct: 661 VVGLEKGCKIDELGPLKDLKGTLTLKNLQNVQNKDEAMAAKLVEKKYLRHLIFQWFLNLY 720
Query: 721 R--ECSSYNDFEVLEGLQPPKNLSSLKITNFGGKFLPAATFVENLVFLCLYGCTKCERLP 780
E ++ +VLEGLQP KN+ SL I F G+ L FVENLV + L C +CE LP
Sbjct: 721 DRGEYDEDDNKQVLEGLQPHKNVQSLDIRGFQGRVLNNNIFVENLVEIRLVDCGRCEVLP 780
Query: 781 MLGQLANLQELSICFMDSVRSIGSEFYGIDSNRRGY--FPKLKKFDFCWMCNLEQWELEV 840
MLGQL NL++L I M+SVRSIGSEFYG+D N R FP+L KF C + L+QW+ E
Sbjct: 781 MLGQLPNLKKLEIISMNSVRSIGSEFYGVDCNDRNSSAFPQLNKFHICGLKKLQQWD-EA 840
Query: 841 ANHESNHFGSLQTLKLDRCGKLTKLPNGLECCKSVHEVIISNCPNLTLNVEEMHNLSVLL 900
SN FG L+ L L C +L KLP+GLE C S+ + I CPNL LNV+ ++NL L
Sbjct: 841 TVFASNRFGCLKELILSGCHQLAKLPSGLEGCYSIEYLAIDGCPNLMLNVQNLYNLYHLD 900
Query: 901 IDGLKFLPKGLALHPNLKTIMIKGCIEDYDYSPFLNLPS-LTKLYLNDGLGNA---TQLP 960
I GLK LP NLK + I GC+++Y++SPF++L S L +L L D + TQLP
Sbjct: 901 IRGLKRLPDEFGKLTNLKKLRIGGCMQNYEFSPFIHLSSQLVELELTDDGSSGSETTQLP 960
Query: 961 KQLQHLTALKILAIENFYGIEVLPEWLRKLTCLETLDLVRCKNLKRLPSRGAMRCLTKLK 1020
+QLQHLT LK+L I +F IEVLPEWL LTCL TL + CKNLK LPSR A++ LTKL
Sbjct: 961 QQLQHLTNLKVLKIADFDDIEVLPEWLGNLTCLATLVFLECKNLKELPSREAIQRLTKLD 1020
Query: 1021 DFKVIACPLLLLGGQADQEGAKYLHIPA 1027
D + CP LLL G+ DQE AK H+P+
Sbjct: 1021 DLVIDGCPKLLL-GEGDQERAKLSHLPS 1035
BLAST of CSPI04G03520 vs. TrEMBL
Match:
A0A0A0LBV0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G822360 PE=3 SV=1)
HSP 1 Score: 927.5 bits (2396), Expect = 1.4e-266
Identity = 547/1060 (51.60%), Postives = 717/1060 (67.64%), Query Frame = 1
Query: 1 MAEFLWTFAVEETLKRTVKVAAQKISLVWGLEDELSNLSKWLLDAGALLRDIDREILRK- 60
MAE + TF V+E LKR VK A++I + W LE+E+S L L DA +L DI+R+
Sbjct: 1 MAE-IGTFVVQEVLKRIVKYGAEQIVVAWELENEVSLLKDKLHDADTILEDINRKKSHPG 60
Query: 61 ESVKRWADGLEDIVSEAEDLLDELAYEDLRRKVETS---SRVCNNFKFSSVLNPLVRHDM 120
SVKRW + LEDIV EA+DLLDEL YE LRR VE + S+V ++ SS+ + L R M
Sbjct: 61 NSVKRWVEKLEDIVHEADDLLDELVYEHLRRTVEHTEKFSKVSDSIS-SSINSFLFRRKM 120
Query: 121 ACKMKKITKMLKQHYRNSAPLGLVGKESMEKEDGGNNLRQIRETTSILNFDVVGRETEVL 180
A K+K IT L QHY ++ GLVG E++ + + L QIRETTSIL+F V GRE EVL
Sbjct: 121 AKKIKNITDTLNQHYCAASAFGLVGVETVTEIELA--LNQIRETTSILDFQVEGREAEVL 180
Query: 181 DILRLVIDSSSNEYELPLLIVPIVGMGGVGKTTLAKLVFRHELIKKHFHETIWICVSEHF 240
++L+L IDS++ + + ++ IVGMGG+GKTTLAK++F H I+ HF +TIW+CVS+ F
Sbjct: 181 ELLKLAIDSTNEHH---MSVISIVGMGGLGKTTLAKMIFNHREIEGHFDKTIWVCVSKPF 240
Query: 241 NIDEILVAILESLTDK---VPTKREAVLRRLQKELLDKRCFLVLDDVWNESSKLWEELED 300
+ +IL I + LT + + +EA+L RL+KE+ DK FLVLDDVW+ LW+EL
Sbjct: 241 IVTKILEKIFQGLTKTCSGLESNKEALLGRLRKEMQDKNYFLVLDDVWDNEKHLWDELRG 300
Query: 301 CLKEIVGKFGITIIVTTRLDEVADIMGTVSGYRLEKLPEDHCWSLFKRSANANGVKMTPK 360
CLK I GK G TI+VTTR +EVA ++ +S YRL+KL D CW+LFK SANAN + M K
Sbjct: 301 CLKHIAGKPGNTIMVTTRNEEVATMVEPISIYRLKKLSNDQCWALFKESANANQLPMNSK 360
Query: 361 LEAIRIKLLQKIDGIPLVAKVLGGAVEF-EGDLDR------WETTLESIVREIPMKQKSY 420
LE ++ +L++K+ G+PLVAKVLGGAV+F E +L+ W T +ESIVR I ++ K +
Sbjct: 361 LEIMKKELVRKMGGVPLVAKVLGGAVKFEETELEEEDHEISWMTKVESIVRNISLEDKDF 420
Query: 421 VLSILQLSVDRLPF-VEKQCFAYCSIFPKDCEVVKENLIRMWIAQGFIQPTEGEN---TM 480
VLSIL+LSVD LP V KQC AYCS F +D + K++LI+MWIAQGFIQP +G + M
Sbjct: 421 VLSILKLSVDSLPNPVLKQCVAYCSNFSQDYDFQKDDLIKMWIAQGFIQPGQGRDKNLLM 480
Query: 481 EDLGEGHFNFLLSRSLFQDVVKDKYGRITHFKMHDLIHDVALAILSTRQKSVLDPTHWNG 540
ED+GE +FNFLLSRS+FQDV +D RI FKMHDL+HD+A AI S+ Q +P + +G
Sbjct: 481 EDIGEQYFNFLLSRSIFQDVTRDANKRIVGFKMHDLMHDIACAI-SSHQNVESNPNNLSG 540
Query: 541 KTSRKLRTLLYNNQEIHH-KVADCVFLRVLEVNSLHMMNNLPDFIAKLKHLRYLDISSCS 600
K+ RKLRTL+ N++ I++ D V LRVL+V +L I KL HLRYLDIS CS
Sbjct: 541 KSVRKLRTLICNDEVINYLNQNDIVCLRVLKV-IFQSHTDLWIPIDKLIHLRYLDISECS 600
Query: 601 M-WVMPHSVTTLFNLQTLKLGSIENLPMNLRNLVRLRHLEFHVYYNTRKMPSHMGELIHL 660
+ ++ S++ L+NLQTLKLG LP NLR LV LRHLEF ++ +T MPS MG LIHL
Sbjct: 601 INKLLLESLSLLYNLQTLKLGQ-SGLPKNLRKLVNLRHLEFKMFGDT-AMPSDMGNLIHL 660
Query: 661 QTLSWFVAGFEEGCKIEELGNLKNLKGQLQLSNLEQVRSKEEALAAKLVNKKNLRELTFE 720
Q+LS F+ GFE+GCKIEELG LKNLKG+L L+NL +V++K+EA+AAKLV KKNLR L
Sbjct: 661 QSLSGFLVGFEKGCKIEELGPLKNLKGKLTLTNLWRVQNKDEAMAAKLVEKKNLRHLNL- 720
Query: 721 WSIDILRECSSYND--FEVLEGLQPPKNLSSLKITNFGGKFLPAATFVENLVFLCLYGCT 780
W + + D +VLEGLQP KNL SL+I F GK LP FVENLV + L
Sbjct: 721 WFFETDKRGEDDEDGIVQVLEGLQPHKNLQSLEILGFRGKVLPTGIFVENLVKIRLGHFE 780
Query: 781 KCERLPMLGQLANLQELSICFMDSVRSIGSEFYGIDSNRRG--YFPKLKKFDFCWMCNLE 840
+CE LPMLGQL NL+EL I +M+SVRSIG+EFYG+DS+ + FP+LKK M NLE
Sbjct: 781 RCEVLPMLGQLPNLKELEIMYMESVRSIGNEFYGVDSSHQNSVAFPQLKKLSIYEMMNLE 840
Query: 841 QWELEVANHESNHFGSLQTLKLDRCGKLTKLPNGLECCKSVHEVIISNCPNLTLNVEEMH 900
QW+ ESN FG L+ +++ RC L KLP+GLE C S+ + I C NL LNV+ +H
Sbjct: 841 QWDEATVVLESNLFGCLKEVRIRRCNPLAKLPSGLEGCHSLEYLSIRGCFNLMLNVQNLH 900
Query: 901 NLSVLLIDGLKFLPKGLALHPNLKTIMIKGCIEDYDYSPFLNLPS-LTKLYLNDGLGNA- 960
L L IDGLK LPKG+ LK + I GC+++Y++S ++L S L +L L+ G+
Sbjct: 901 KLYHLEIDGLKRLPKGMDGLTRLKELKIGGCMQNYEFSSVIHLASQLVELELSGRYGSVD 960
Query: 961 TQLPKQLQHLTALKILAIENFYGIEVLPEWLRKLTCLETLDLVRCKNLKRLPSRGAMRCL 1020
TQLP+QLQHLT L++L I F IE LPEW+ L L+TL C LK LPSR A+ L
Sbjct: 961 TQLPQQLQHLTNLQVLKITQFDCIEALPEWIGNLISLKTLKCSYCFKLKELPSREAILRL 1020
Query: 1021 TKLKDFKVIACPLLLLGGQADQEGAKYLHIPAYLCHVYQS 1035
TKL++ + CP LL+ G+ DQE AK H+P+ H +S
Sbjct: 1021 TKLENLDIFECPKLLV-GEGDQERAKLSHLPSKCVHKSES 1047
BLAST of CSPI04G03520 vs. TrEMBL
Match:
Q2V727_CUCME (R-FOM-2 OS=Cucumis melo PE=3 SV=1)
HSP 1 Score: 911.0 bits (2353), Expect = 1.3e-261
Identity = 525/1077 (48.75%), Postives = 708/1077 (65.74%), Query Frame = 1
Query: 1 MAEFLWTFAVEETLKRTVKVAAQKISLVWGLEDELSNLSKWLLDAGALLRDIDREILRKE 60
M +FLWTFAVEE LK+ +KVA ++ L WG + LS L KWLL A A LR+I+ L +
Sbjct: 1 MGDFLWTFAVEEMLKKVLKVAREQTGLAWGFQKHLSKLQKWLLKAEAFLRNINTRKLHHD 60
Query: 61 SVKRWADGLEDIVSEAEDLLDELAYEDLRRKVETSS--RVCNNFKFSSVLNPLV-RHDMA 120
SV+ W D L +V +A+DLLDE+ YEDLR+KV+T +VC+ F S+ N L+ R +MA
Sbjct: 61 SVRMWVDDLRHLVYQADDLLDEIVYEDLRQKVQTRKMKKVCDFFSPST--NVLIFRLNMA 120
Query: 121 CKMKKITKMLKQHYRNSAPLGLVGKESMEKEDGGNNLRQIRETTSIL-NFDVVGRETEVL 180
KM + +L++HY +APLGLVG E++ E + + Q RET S L + ++GR+ EV
Sbjct: 121 KKMMTLIALLEKHYLEAAPLGLVGNENVSPEI--DVISQYRETISELEDHKILGRDVEVE 180
Query: 181 DILRLVIDSSSNEYELPLLIVPIVGMGGVGKTTLAKLVFRHELIKKHFHETIWICVSEHF 240
I++ VID+S+N+ I+PIVGMGG+GKTTLAKLVF+HEL+++HF +T+W+CVSE F
Sbjct: 181 SIVKQVIDASNNQLTS---ILPIVGMGGLGKTTLAKLVFKHELVRQHFDKTVWVCVSEPF 240
Query: 241 NIDEILVAILESLTDKVPT---KREAVLRRLQKELLDKRCFLVLDDVWNESSKLWEELED 300
+++IL+ IL++L + +E +LR LQKE+L + FLVLDDVWNE+S LW EL+
Sbjct: 241 IVNKILLDILQNLKGGISNGGDSKEVLLRELQKEMLGQTYFLVLDDVWNENSFLWGELKY 300
Query: 301 CLKEIVGKFGITIIVTTRLDEVADIMGTVSGYRLEKLPEDHCWSLFKRSANANGVKMTPK 360
CL +I G +I+VTTR EV IMGT G+ L KL +DHCWSLFK SAN G+ MT
Sbjct: 301 CLLKITGNSKNSIVVTTRSAEVTKIMGTCPGHLLSKLSDDHCWSLFKESANVYGLSMTSN 360
Query: 361 LEAIRIKLLQKIDGIPLVAKVLGGAVEFEGDLDRWETTLESIVREIPMKQKSYVLSILQL 420
L I+ +L++KI G+PLVA+VLG V+FEGD+++WE TL+S++R IP++++ +VLSIL+L
Sbjct: 361 LGIIQKELVKKIGGVPLVARVLGRTVKFEGDVEKWEETLKSVLR-IPVQEEDFVLSILKL 420
Query: 421 SVDRLP-FVEKQCFAYCSIFPKDCEVVKENLIRMWIAQGFIQPTEGEN-TMEDLGEGHFN 480
SVDRLP KQCF+YCSIFPKD K+ LI+MW+AQGF+QP EG N TME +G+ +F
Sbjct: 421 SVDRLPSSALKQCFSYCSIFPKDFVFEKQELIQMWMAQGFLQPQEGRNMTMETVGDIYFK 480
Query: 481 FLLSRSLFQDVVKDK----------YG-RITHFKMHDLIHDVALAILSTRQKSVLDPTHW 540
LLS LFQD + + YG R +KMHDL+HD+A+AI S Q L+P++
Sbjct: 481 ILLSHCLFQDAHETRTEEYKMHDLVYGTRTEEYKMHDLVHDIAMAI-SRDQNLQLNPSNI 540
Query: 541 NGK---------TSRKLRTLLYNNQEIHH---------KVADCVFLRVLEVNSLHMMNNL 600
+ K + KLRT+ + + H+ K+ + V LR+L+++ + L
Sbjct: 541 SEKELQKKEIKNVACKLRTIDFIQKIPHNIGQLTFFDVKIRNFVCLRILKISKM-SSEKL 600
Query: 601 PDFIAKLKHLRYLDISSCSMWV-MPHSVTTLFNLQTLKL--GSIENLPMNLRNLVRLRHL 660
P I +LKHLRYL+I+S S + P S+ +L NLQTLK +E PMN NLV LRHL
Sbjct: 601 PKSIDQLKHLRYLEIASYSTRLKFPESIVSLHNLQTLKFLYSFVEEFPMNFSNLVNLRHL 660
Query: 661 EFHVYYNTRKMPSHMGELIHLQTLSWFVAGFEEGCKIEELGNLKNLKGQLQLSNLEQVRS 720
+ ++ N + P H+ +L LQTLS FV GFEEGCKI ELG LKNL+G L LE+V S
Sbjct: 661 K--LWRNVDQTPPHLSQLTQLQTLSHFVIGFEEGCKIIELGPLKNLQGSSNLLCLEKVES 720
Query: 721 KEEALAAKLVNKKNLRELTFEWSIDILRECSSYNDFEVLEGLQPPKNLSSLKITNFGGKF 780
KEEA A L K+NL+EL WS+ ++ +YND EVLEGLQP +NL L+I +F +
Sbjct: 721 KEEAKGANLAEKENLKELNLSWSMK-RKDNDNYNDLEVLEGLQPNQNLQILRIHDFTERR 780
Query: 781 LPAATFVENLVFLCLYGCTKCERLPMLGQLANLQELSICFMDSVRSIGSEFYGIDSNRRG 840
LP FVENL+ + LYGC CE+LPMLGQL NL++L IC D V+ I ++FYG D N+R
Sbjct: 781 LPNKIFVENLIEIGLYGCDNCEKLPMLGQLNNLKKLEICSFDGVQIIDNKFYGNDPNQRR 840
Query: 841 YFPKLKKFDFCWMCNLEQWELEVANHESNH---FGSLQTLKLDRCGKLTKLPNGLECCKS 900
+FPKL+KF M NLEQWE + N S++ F +L++L++ C KLTK+PNGL+ C S
Sbjct: 841 FFPKLEKFVMQNMINLEQWEEVMTNDASSNVTIFPNLKSLEISGCPKLTKIPNGLQFCSS 900
Query: 901 VHEVIISNCPNLTLNVEEMHNLSVLLIDGLKFLPKGLALHPNLKTIMIKGCIEDYDYSPF 960
+ V I C NL +N+ L L I L LP+ L NL + I G I++YD+
Sbjct: 901 IRRVKIYQCSNLGINMRNKPELWYLHIGPLGKLPEDLCHLMNLGVMTIVGNIQNYDFGIL 960
Query: 961 LNLPSLTKLYL-NDGLGN--ATQLPKQLQHLTALKILAIENFYGIEVLPEWLRKLTCLET 1020
+LPSL K+ L D L N TQ+P+QLQHLT+L+ L+IENF GIE LPEWL L CL+T
Sbjct: 961 QHLPSLKKITLVEDELSNNSVTQIPQQLQHLTSLEFLSIENFGGIEALPEWLGNLVCLQT 1020
Query: 1021 LDLVRCKNLKRLPSRGAMRCLTKLKDFKVIACPLLLLGGQADQEGAKYLHIPAYLCH 1031
L + C+NLK+LPS AM LTKL CP+LLL + D E AK H P L H
Sbjct: 1021 LCFLCCRNLKKLPSTEAMLRLTKLNKLYACECPMLLL-EEGDPERAKLSHFPNVLAH 1063
BLAST of CSPI04G03520 vs. TrEMBL
Match:
Q6E436_CUCME (FOM-2 OS=Cucumis melo GN=Fom-2 PE=3 SV=1)
HSP 1 Score: 909.4 bits (2349), Expect = 3.9e-261
Identity = 526/1078 (48.79%), Postives = 706/1078 (65.49%), Query Frame = 1
Query: 1 MAEFLWTFAVEETLKRTVKVAAQKISLVWGLEDELSNLSKWLLDAGALLRDIDREILRKE 60
M +FLWTFAVEE LK+ +KVA ++ L WG + LS L KWLL A A LR+I+ L +
Sbjct: 1 MGDFLWTFAVEEMLKKVLKVAREQAGLAWGFQKHLSKLQKWLLKAEAFLRNINTRKLHHD 60
Query: 61 SVKRWADGLEDIVSEAEDLLDELAYEDLRRKVETSS--RVCNNFKFSSVLNPLV-RHDMA 120
SV+ W D L +V +A+DLLDE+ YE LR+KV+T +VC+ F S+ N L+ R +MA
Sbjct: 61 SVRMWVDDLRHLVYQADDLLDEIVYEHLRQKVQTRKMKKVCDFFSPST--NVLIFRLNMA 120
Query: 121 CKMKKITKMLKQHYRNSAPLGLVGKESMEKEDGGNNLRQIRETTSIL-NFDVVGRETEVL 180
KM + +L++HY +APLGLVG E++ E + + Q RET S L + +VGR+ EV
Sbjct: 121 KKMMTLIALLEKHYLEAAPLGLVGNENVRPEI--DVISQYRETISELEDHKIVGRDVEVE 180
Query: 181 DILRLVIDSSSNEYELPLLIVPIVGMGGVGKTTLAKLVFRHELIKKHFHETIWICVSEHF 240
I++ VID+S+N+ I+PIVGMGG+GKTTLAKLVF HEL+++HF +T+W+CVSE F
Sbjct: 181 SIVKQVIDASNNQLTS---ILPIVGMGGLGKTTLAKLVFSHELVRQHFDKTVWVCVSEPF 240
Query: 241 NIDEILVAILESLTDKVPT---KREAVLRRLQKELLDKRCFLVLDDVWNESSKLWEELED 300
+++IL+ IL+SL + +E +LR LQKE+L + FLVLDDVWNE+S LW EL+
Sbjct: 241 IVNKILLDILQSLKGGISNGGDSKEVLLRELQKEMLGQTYFLVLDDVWNENSFLWGELKY 300
Query: 301 CLKEIVGKFGITIIVTTRLDEVADIMGTVSGYRLEKLPEDHCWSLFKRSANANGVKMTPK 360
CL +I G +I+VTTR EVA IMGT G+ L KL +DHCWSLFK SAN G+ MT
Sbjct: 301 CLLKITGNSKNSIVVTTRSAEVAKIMGTCPGHLLSKLSDDHCWSLFKESANVYGLSMTSN 360
Query: 361 LEAIRIKLLQKIDGIPLVAKVLGGAVEFEGDLDRWETTLESIVREIPMKQKSYVLSILQL 420
L I+ +L++KI G+PLVA+VLG V+FEGD+++WE TL+S++R IP++++ +VLSIL+L
Sbjct: 361 LGIIQKELVKKIGGVPLVAQVLGRTVKFEGDVEKWEETLKSVLR-IPVQEEDFVLSILKL 420
Query: 421 SVDRLP-FVEKQCFAYCSIFPKDCEVVKENLIRMWIAQGFIQPTEGEN-TMEDLGEGHFN 480
SVDRLP KQCF+YCSIFPKD K+ LI+MW+AQGF+QP EG N TME +G+ +F
Sbjct: 421 SVDRLPSSALKQCFSYCSIFPKDFVFEKQELIQMWMAQGFLQPQEGRNMTMETVGDIYFK 480
Query: 481 FLLSRSLFQDVVKDK----------YG-RITHFKMHDLIHDVALAILSTRQKSVLDPTHW 540
LLS LFQD + + YG R +KMHDL+HD+A+AI S Q L+P++
Sbjct: 481 ILLSHCLFQDAHETRTEEYKMHDLVYGTRTEEYKMHDLVHDIAMAI-SRDQNLQLNPSNI 540
Query: 541 NGK---------TSRKLRTLLYNNQEIHHKVADCVF----------LRVLEVNSLHMMNN 600
+ K + KLRT+ + NQ+I H + +F LR+L+++ +
Sbjct: 541 SKKELQKKEIKNVACKLRTIDF-NQKIPHNIGQLIFFDVKIRNFVCLRILKISKV-SSEK 600
Query: 601 LPDFIAKLKHLRYLDISSCSMWV-MPHSVTTLFNLQTLKL--GSIENLPMNLRNLVRLRH 660
LP I +LKHLRYL+I+S S + P S+ +L NLQTLK +E PMN NLV LRH
Sbjct: 601 LPKSIDQLKHLRYLEIASYSTRLKFPESIVSLHNLQTLKFLYSFVEEFPMNFSNLVSLRH 660
Query: 661 LEFHVYYNTRKMPSHMGELIHLQTLSWFVAGFEEGCKIEELGNLKNLKGQLQLSNLEQVR 720
L+ ++ N + P H+ +L LQTLS FV GFEEG KI ELG LKNL+ L L LE+V
Sbjct: 661 LK--LWGNVEQTPPHLSQLTQLQTLSHFVIGFEEGRKIIELGPLKNLQDSLNLLCLEKVE 720
Query: 721 SKEEALAAKLVNKKNLRELTFEWSIDILRECSSYNDFEVLEGLQPPKNLSSLKITNFGGK 780
SKEEA A L K+NL+EL WS+ ++ SYND EVLEGLQP +NL L+I +F +
Sbjct: 721 SKEEAKGANLAEKENLKELNLSWSMK-RKDNDSYNDLEVLEGLQPNQNLQILRIHDFTER 780
Query: 781 FLPAATFVENLVFLCLYGCTKCERLPMLGQLANLQELSICFMDSVRSIGSEFYGIDSNRR 840
LP FVENL+ + LYGC C++LPMLGQL NL++L IC D V+ I +EFYG D N+R
Sbjct: 781 RLPNKIFVENLIEIGLYGCDNCKKLPMLGQLNNLKKLEICSFDGVQIIDNEFYGNDPNQR 840
Query: 841 GYFPKLKKFDFCWMCNLEQWELEVANHESNH---FGSLQTLKLDRCGKLTKLPNGLECCK 900
+FPKL+KF M NLEQWE + N S++ F +L++L++ C KLTK+PNGL C
Sbjct: 841 RFFPKLEKFAMGGMMNLEQWEEVMTNDASSNVTIFPNLRSLEIRGCPKLTKIPNGLHFCS 900
Query: 901 SVHEVIISNCPNLTLNVEEMHNLSVLLIDGLKFLPKGLALHPNLKTIMIKGCIEDYDYSP 960
S+ V I C NL++N+ L L I L LP+ L NL + I G I++YD+
Sbjct: 901 SIRRVKIYKCSNLSINMRNKLELWYLHIGPLDKLPEDLCHLMNLGVMTIVGNIQNYDFGI 960
Query: 961 FLNLPSLTKLYLNDGL---GNATQLPKQLQHLTALKILAIENFYGIEVLPEWLRKLTCLE 1020
+LPSL K+ L +G + Q+P+QLQHLT+L+ L+IENF GIE LPEWL L CL+
Sbjct: 961 LQHLPSLKKITLVEGKLSNNSVKQIPQQLQHLTSLEFLSIENFGGIEALPEWLGNLVCLQ 1020
Query: 1021 TLDLVRCKNLKRLPSRGAMRCLTKLKDFKVIACPLLLLGGQADQEGAKYLHIPAYLCH 1031
TL + C+NLK+LPS AM LTKL CP+LLL + D E AK H P L H
Sbjct: 1021 TLCFLCCRNLKKLPSTEAMLRLTKLNKLYACECPMLLL-EEGDPERAKLSHFPNVLAH 1063
BLAST of CSPI04G03520 vs. TAIR10
Match:
AT3G14470.1 (AT3G14470.1 NB-ARC domain-containing disease resistance protein)
HSP 1 Score: 348.6 bits (893), Expect = 1.3e-95
Identity = 299/1019 (29.34%), Postives = 480/1019 (47.11%), Query Frame = 1
Query: 32 EDELSNLSKWLLDAGALLRDIDREILRKESVKRWADGLEDIVSEAEDLLDELAYEDLRRK 91
E+ L LS LL A+L D + + + V++W + L D+V AED LD++A E LR
Sbjct: 36 ENLLERLSTALLTITAVLIDAEEKQITNPVVEKWVNELRDVVYHAEDALDDIATEALRLN 95
Query: 92 VETSSRVCNNFK-------FSSVLNPLVRHDMACKMKKITKMLKQHYRNSAPLGLVGKES 151
+ S N + L+ H + +++K+T L++ LGL
Sbjct: 96 IGAESSSSNRLRQLRGRMSLGDFLDGNSEH-LETRLEKVTIRLERLASQRNILGL----- 155
Query: 152 MEKEDGGNNLRQIRETTSILN-FDVVGRETEVLDILRLVIDSSSNEYELPLLIVPIVGMG 211
KE +Q TTS+++ +V GR+ + +I+R +I + + + +V IVG+G
Sbjct: 156 --KELTAMIPKQRLPTTSLVDESEVFGRDDDKDEIMRFLIPENGKDNGIT--VVAIVGIG 215
Query: 212 GVGKTTLAKLVFRHELIKKHFHETIWICVSEHFNIDEILVAILESLTDKVP--TKREAVL 271
GVGKTTL++L++ + ++ +F +W VSE F++ +I + ES+T + T + +
Sbjct: 216 GVGKTTLSQLLYNDQHVRSYFGTKVWAHVSEEFDVFKITKKVYESVTSRPCEFTDLDVLQ 275
Query: 272 RRLQKELLDKRC--FLVLDDVWNESSKLWEELEDCLKEIVGKFGITIIVTTRLDEVADIM 331
+L++ L LVLDD+WNE+ W+ L I G I+VTTR VA IM
Sbjct: 276 VKLKERLTGTGLPFLLVLDDLWNENFADWDLLRQPF--IHAAQGSQILVTTRSQRVASIM 335
Query: 332 GTVSGYRLEKLPEDHCWSLFKRSANANGVK-MTPKLEAIRIKLLQKIDGIPLVAKVLGGA 391
V + L+ L + CWSLF ++ N + ++ + +++ K G+PL K LGG
Sbjct: 336 CAVHVHNLQPLSDGDCWSLFMKTVFGNQEPCLNREIGDLAERIVHKCRGLPLAVKTLGGV 395
Query: 392 VEFEGDLDRWETTLESIVREIPMKQKSYVLSILQLSVDRLPFVEKQCFAYCSIFPKDCEV 451
+ FEG + WE L S + ++P KS +L +L++S LP K+CFAYCSIFPK
Sbjct: 396 LRFEGKVIEWERVLSSRIWDLPA-DKSNLLPVLRVSYYYLPAHLKRCFAYCSIFPKGHAF 455
Query: 452 VKENLIRMWIAQGFIQPTEGENTMED---------LGEGHFNFLLSRSLFQDVVKD---- 511
K+ ++ +W+A+GF+Q T +E+ +R + D + +
Sbjct: 456 EKDKVVLLWMAEGFLQQTRSSKNLEELGNEYFSELESRSLLQKTKTRYIMHDFINELAQF 515
Query: 512 KYGRITHFKMHD---LIHDVALAILSTRQKSVLDPTHWNGKTSRK-LRTLL--------- 571
G + K D L LS + + +P + K LRT L
Sbjct: 516 ASGEFSS-KFEDGCKLQVSERTRYLSYLRDNYAEPMEFEALREVKFLRTFLPLSLTNSSR 575
Query: 572 --YNNQEIHHKVADCVF-LRVLEVNSLHMMNNLPDFIAKLKHLRYLDISSCSMWVMPHSV 631
+Q + K+ + LRVL ++ + PDF + H R+LD+S + +P S+
Sbjct: 576 SCCLDQMVSEKLLPTLTRLRVLSLSHYKIARLPPDFFKNISHARFLDLSRTELEKLPKSL 635
Query: 632 TTLFNLQTLKLG---SIENLPMNLRNLVRLRHLEFHVYYNTRKMPSHMGELIHLQTLSWF 691
++NLQTL L S++ LP ++ NL+ LR+L+ + R+MP G L LQTL+ F
Sbjct: 636 CYMYNLQTLLLSYCSSLKELPTDISNLINLRYLDL-IGTKLRQMPRRFGRLKSLQTLTTF 695
Query: 692 VAGFEEGCKIEELGNLKNLKGQLQLSNLEQVRSKEEALAAKLVNKKNLRELTFEW----- 751
+G +I ELG L +L G+L++ L++V +A A L +KK+LRE+ F W
Sbjct: 696 FVSASDGSRISELGGLHDLHGKLKIVELQRVVDVADAAEANLNSKKHLREIDFVWRTGSS 755
Query: 752 -SIDILRECSSYNDFEVLEGLQPPKNLSSLKITNFGGKFLP---AATFVENLVFLCLYGC 811
S + + N+ EV E L+P +++ L I + G+ P + +V + L C
Sbjct: 756 SSENNTNPHRTQNEAEVFEKLRPHRHIEKLAIERYKGRRFPDWLSDPSFSRIVCIRLREC 815
Query: 812 TKCERLPMLGQLANLQELSICFMDSVRSIGSEFYGIDSNRRGY----FPKLKKFDFCWMC 871
C LP LGQL L+EL I M ++SIG +FY D R F L+ F +
Sbjct: 816 QYCTSLPSLGQLPCLKELHISGMVGLQSIGRKFYFSDQQLRDQDQQPFRSLETLRFDNLP 875
Query: 872 NLEQWELEVANHESNHFGSLQTLKLDRCGKLT-KLPNGLECCKSVHEVIISNCPNLTLNV 931
+ ++W L+V + F SL+ L + RC +LT LP L S+H I C L
Sbjct: 876 DWQEW-LDVRVTRGDLFPSLKKLFILRCPELTGTLPTFLPSLISLH---IYKCGLLDFQP 935
Query: 932 EEMHNLSVLLIDGLKFLPKGLALHPNLKTIMIKGCIEDYDYSPFLNLPSLTKLYLNDGLG 991
+ H S + NL+T+ IK + +L K LN
Sbjct: 936 DH-HEYS----------------YRNLQTLSIKSSCD-----------TLVKFPLN---- 995
BLAST of CSPI04G03520 vs. TAIR10
Match:
AT3G14460.1 (AT3G14460.1 LRR and NB-ARC domains-containing disease resistance protein)
HSP 1 Score: 316.2 bits (809), Expect = 7.4e-86
Identity = 262/890 (29.44%), Postives = 426/890 (47.87%), Query Frame = 1
Query: 35 LSNLSKWLLDAGALLRDIDREILRKESVKRWADGLEDIVSEAEDLLDELAYEDLRRKVET 94
L L L+ A +L D D+ VK W G++D +AED+LDEL E LRR+V
Sbjct: 36 LKRLKVALVTANPVLADADQRAEHVREVKHWLTGIKDAFFQAEDILDELQTEALRRRVVA 95
Query: 95 SSRVCNNFKFSSVL--NPLVRHDMACKMKKITKMLKQHYRNSAPLGLVGKESMEKEDGGN 154
+ F +++ ++ + KM+K+ ++L+ H ++ +GL KE E +
Sbjct: 96 EAGGLGGL-FQNLMAGREAIQKKIEPKMEKVVRLLEHHVKHIEVIGL--KEYSETREPQW 155
Query: 155 NLRQIRETTSILNFDVVGRETEVLDILRLVIDSSSNEYELPLLIVPIVGMGGVGKTTLAK 214
+ +VGR + L ++ L++ P ++ +VGM GVGKTTL +
Sbjct: 156 RQASRSRPDDLPQGRLVGRVEDKLALVNLLLSDDEISIGKPA-VISVVGMPGVGKTTLTE 215
Query: 215 LVFRHELIKKHFHETIWICVSEHFNIDEILVAILESLTDKVPTKRE--AVLRRLQKELLD 274
+VF + +HF +WI +FN+ + A+L+ +T + ++ +L+K L
Sbjct: 216 IVFNDYRVTEHFEVKMWISAGINFNVFTVTKAVLQDITSSAVNTEDLPSLQIQLKKTLSG 275
Query: 275 KRCFLVLDDVWNESSKLWEELEDCLKEIVGKFGITIIVTTRLDEVADIMGTVSGYRLEKL 334
KR LVLDD W+ES WE + + + G I++TTR + V+ + Y+++ +
Sbjct: 276 KRFLLVLDDFWSESDSEWESFQVAFTD--AEEGSKIVLTTRSEIVSTVAKAEKIYQMKLM 335
Query: 335 PEDHCWSLFKRSA--NANGVKMTPKLEAIRIKLLQKIDGIPLVAKVLGGAVEFEGDLDRW 394
+ CW L R A N + + +LE I ++ ++ G+PL A+ + + + + D W
Sbjct: 336 TNEECWELISRFAFGNISVGSINQELEGIGKRIAEQCKGLPLAARAIASHLRSKPNPDDW 395
Query: 395 ETTLESIVREIPMKQKSYVLSILQLSVDRLPFVEKQCFAYCSIFPKDCEVVKENLIRMWI 454
++ + +L +L+LS D LP K+CFA CSIFPK +E L+ +W+
Sbjct: 396 YAVSKNF-----SSYTNSILPVLKLSYDSLPPQLKRCFALCSIFPKGHVFDREELVLLWM 455
Query: 455 AQGFIQPTEGENTMEDLGEGHFNFLLSRSLFQ--DVVKDKYGRITHFKMHDLIHDVALA- 514
A + +ED+G + L+++S FQ D+ +T F MHDL++D+A A
Sbjct: 456 AIDLLYQPRSSRRLEDIGNDYLGDLVAQSFFQRLDIT------MTSFVMHDLMNDLAKAV 515
Query: 515 ---------------ILSTRQKSVLDPTHWNGKTSRK-------LRTLLYNNQ------- 574
I ST + + + + + LRT+L N
Sbjct: 516 SGDFCFRLEDDNIPEIPSTTRHFSFSRSQCDASVAFRSICGAEFLRTILPFNSPTSLESL 575
Query: 575 EIHHKVADCVF-----LRVLEVNSLHMMNNLPDFIAKLKHLRYLDISSCSMWVMPHSVTT 634
++ KV + + LR+L + S + + NLP + LK LRYLD+SS + +P V T
Sbjct: 576 QLTEKVLNPLLNALSGLRILSL-SHYQITNLPKSLKGLKLLRYLDLSSTKIKELPEFVCT 635
Query: 635 LFNLQTLKLGS---IENLPMNLRNLVRLRHLEFHVYYNTRKMPSHMGELIHLQTLSWFVA 694
L NLQTL L + + +LP ++ L+ LR L+ V +MP + +L LQ LS FV
Sbjct: 636 LCNLQTLLLSNCRDLTSLPKSIAELINLRLLDL-VGTPLVEMPPGIKKLRSLQKLSNFVI 695
Query: 695 GFEEGCKIEELGNLKNLKGQLQLSNLEQVRSKEEALAAKLVNKKNLRELTFEWSIDILRE 754
G G + EL L +L+G L++S L+ V EA A L K L L +W++
Sbjct: 696 GRLSGAGLHELKELSHLRGTLRISELQNVAFASEAKDAGLKRKPFLDGLILKWTVKGSGF 755
Query: 755 C-SSYNDF-----EVLEGLQPPKNLSSLKITNFGG----KFLPAATFVENLVFLCLYGCT 814
S+N EVL L+P +L + I ++ G K+L ++F + + L C
Sbjct: 756 VPGSFNALACDQKEVLRMLEPHPHLKTFCIESYQGGAFPKWLGDSSFF-GITSVTLSSCN 815
Query: 815 KCERLPMLGQLANLQELSICFMDSVRSIGSEFYGIDSNRRGY-FPKLKKFDFCWMCNLEQ 867
C LP +GQL +L+ LSI + ++ +G +F+ ++N RG F L+ F M ++
Sbjct: 816 LCISLPPVGQLPSLKYLSIEKFNILQKVGLDFFFGENNSRGVPFQSLQILKFYGMPRWDE 875
BLAST of CSPI04G03520 vs. TAIR10
Match:
AT1G53350.1 (AT1G53350.1 Disease resistance protein (CC-NBS-LRR class) family)
HSP 1 Score: 234.6 bits (597), Expect = 2.8e-61
Identity = 237/926 (25.59%), Postives = 422/926 (45.57%), Query Frame = 1
Query: 1 MAEFLWTFAVEETLKRTVKVAAQKISLVWGLEDELSNLSKWLLDAGALLRDIDREILRKE 60
MAE + +F VE+ + + +A+ + G+++++ L + L +LL+D D + E
Sbjct: 1 MAEAVVSFGVEKLWELLSRESAR----LNGIDEQVDGLKRQLGRLQSLLKDADAKKNETE 60
Query: 61 SVKRWADGLEDIVSEAEDLLDELAYEDLRRKVETSSRVCNNFKFSSVLNPLVRHDMACKM 120
V+ + + ++DIV +A+D+++ +LR K + + V R A +
Sbjct: 61 RVRNFLEDVKDIVYDADDIIESFLLNELRGKEKGIKKQVRTLACFLV----DRRKFASDI 120
Query: 121 KKITKMLKQHYRNSAPLGLV-----GKESMEKEDGGNNLRQIRETTSILNFDVVGRETEV 180
+ ITK + + LG+ G S+ ++ +RQ S D+VG + V
Sbjct: 121 EGITKRISEVIVGMQSLGIQHIADGGGRSLSLQERQREIRQTFSRNS--ESDLVGLDQSV 180
Query: 181 LDILRLVIDSSSNEYELPLLIVPIVGMGGVGKTTLAKLVFRHELIKKHFHETIWICVSEH 240
+++ ++++ S + +V + GMGG+GKTTLA+ VF H+++++HF W+CVS+
Sbjct: 181 EELVDHLVENDSVQ------VVSVSGMGGIGKTTLARQVFHHDIVRRHFDGFSWVCVSQQ 240
Query: 241 FNIDEILVAILESLT---DKVPTKREAVLRRLQKELLDK-RCFLVLDDVWNESSKLWEEL 300
F ++ IL+ L + + E L+ ELL+ R LVLDDVW E W+ +
Sbjct: 241 FTRKDVWQRILQDLRPYDEGIIQMDEYTLQGELFELLESGRYLLVLDDVWKEED--WDRI 300
Query: 301 EDCLKEIVGKFGITIIVTTRLDEVA-DIMGTVSGYRLEKLPEDHCWSLFKRSANANGVKM 360
+ K G +++T+R + + T +R L + W LF+R ++ K
Sbjct: 301 KAVFPH---KRGWKMLLTSRNEGLGLHADPTCFAFRPRILTPEQSWKLFERIVSSRRDKT 360
Query: 361 TPKL-EAIRIKLLQKIDGIPLVAKVLGGAVEFEGDLDRWETTLESIVREIPMKQ------ 420
K+ EA+ +++ G+PL KVLGG + + + W+ +IV I K
Sbjct: 361 EFKVDEAMGKEMVTYCGGLPLAVKVLGGLLAKKHTVLEWKRVHSNIVTHIVGKSGLSDDN 420
Query: 421 KSYVLSILQLSVDRLPFVEKQCFAYCSIFPKDCEVVKENLIRMWIAQGFIQPTEGENTME 480
+ V +L LS + LP K CF Y + FP+D ++ + L W+A+G I P +T++
Sbjct: 421 SNSVYRVLSLSYEDLPMQLKHCFFYLAHFPEDYKIDVKILFNYWVAEGIITPFHDGSTIQ 480
Query: 481 DLGEGHFNFLLSRSLFQDVVKDKY--GRITHFKMHDLIHDVALA-------ILSTRQKSV 540
D GE + L+ R++ VV++ Y RI + +MHD++ +V L+ I + +
Sbjct: 481 DTGESYLEELVRRNMV--VVEESYLTSRIEYCQMHDMMREVCLSKAKEENFIRVVKVPTT 540
Query: 541 LDPTHWNGKTSRKLRTLLYNNQEIH------HKVADCV-------------------FLR 600
T R R +L++ +H +K A V LR
Sbjct: 541 TSTTINAQSPCRSRRLVLHSGNALHMLGHKDNKKARSVLIFGVEEKFWKPRGFQCLPLLR 600
Query: 601 VLEVNSLHMM-NNLPDFIAKLKHLRYLDISSCSMWVMPHSVTTLFNLQTLKLGSIENL-- 660
VL+++ + LP I L HLR+L + + +P S+ L L L LG + L
Sbjct: 601 VLDLSYVQFEGGKLPSSIGDLIHLRFLSLYEAGVSHLPSSLGNLKLLLCLNLGVADRLLV 660
Query: 661 --PMNLRNLVRLRHLEFHVYYNTRKMPS----HMGELIHLQTLSWFVAGFEEGCKIEELG 720
P L+ + LR+L R MP+ +G+L++L++L+ F + G
Sbjct: 661 HVPNVLKEMQELRYLRL-----PRSMPAKTKLELGDLVNLESLTNFST---------KHG 720
Query: 721 NLKNLKGQLQLSNLEQVRSKE---EALAAKLVNKKNLRELTFEWSIDILRECSSYNDFEV 780
++ +L +LS L + S E E L L +NL L+F + + + V
Sbjct: 721 SVTDLLRMTKLSVLNVIFSGECTFETLLLSLRELRNLETLSFH-DFQKVSVANHGGELLV 780
Query: 781 LEGLQPPKNLSSLKITNFGGKFLPAATFVENLVFLCLYGCTKCE-RLPMLGQLANLQELS 840
L+ + +L L ++ +F F +L + L GC E +P+L +L +L+ +
Sbjct: 781 LDFI----HLKDLTLSMHLPRFPDQYRFPPHLAHIWLIGCRMEEDPMPILEKLLHLKSV- 840
Query: 841 ICFMDSVRSIGSEFYGIDSNRRGYFPKLKKFDFCWMCNLEQWELEVANHESNHFGSLQTL 863
++ S +G +G FP+L + L +W + E L+TL
Sbjct: 841 --YLSSGAFLGRRMV----CSKGGFPQLLALKMSYKKELVEWRV-----EEGSMPCLRTL 872
BLAST of CSPI04G03520 vs. TAIR10
Match:
AT3G50950.2 (AT3G50950.2 HOPZ-ACTIVATED RESISTANCE 1)
HSP 1 Score: 229.6 bits (584), Expect = 9.1e-60
Identity = 217/771 (28.15%), Postives = 362/771 (46.95%), Query Frame = 1
Query: 14 LKRTVKVAAQKISLVWGLEDELSNLSKWLLDAGALLRDIDREILRKESVKRWADGLEDIV 73
L++T+ + +K V +L +L L + L+D +R+ E+++ L ++V
Sbjct: 10 LEKTLNILEEKGRTVSDYRKQLEDLQSELKYMQSFLKDAERQKRTNETLRTLVADLRELV 69
Query: 74 SEAEDLLDELAYEDLRRKVETSSRVCNNFKFSSVLNPLVRHDMACKMKKITKMLKQHYRN 133
EAED+L + D E S + PL ++ + ++++I + + +
Sbjct: 70 YEAEDILVDCQLADGDDGNEQRSSNAWLSRLHPARVPL-QYKKSKRLQEINERITKIKSQ 129
Query: 134 SAPLGLVGKESMEKEDGGNNLRQIRETTSILNF-DVVGRETEVLDILRLVIDSSSNEYEL 193
P S D G + R ++ + + VVG E + I + S+ ++
Sbjct: 130 VEPYFEFITPSNVGRDNGTD----RWSSPVYDHTQVVGLEGDKRKIKEWLFRSNDSQ--- 189
Query: 194 PLLIVPIVGMGGVGKTTLAKLVFRHELIKKHFHETIWICVSEHFNIDEILVAILESLTD- 253
LLI+ VGMGG+GKTT+A+ VF + I+ F IW+ VS+ F ++I+ +IL +L D
Sbjct: 190 -LLIMAFVGMGGLGKTTIAQEVFNDKEIEHRFERRIWVSVSQTFTEEQIMRSILRNLGDA 249
Query: 254 KVPTKREAVLRRLQKELLDKRCFLVLDDVWNESSKLWEELEDCLKEIVGKFGITIIVTTR 313
V +LR++Q+ LL KR +V+DDVW+++ W+++ L G+ G ++IVTTR
Sbjct: 250 SVGDDIGTLLRKIQQYLLGKRYLIVMDDVWDKNLSWWDKIYQGLPR--GQGG-SVIVTTR 309
Query: 314 LDEVADIMGTVSG--YRLEKLPEDHCWSLFKRSANA--NGVKMTPKLEAIRIKLLQKIDG 373
+ VA + +R E L D+ W LF A A +G P+LE + +++ K G
Sbjct: 310 SESVAKRVQARDDKTHRPELLSPDNSWLLFCNVAFAANDGTCERPELEDVGKEIVTKCKG 369
Query: 374 IPLVAKVLGGAVEFEGDL-DRWETTLESIVREI--PMKQKSYVLSILQLSVDRLPFVEKQ 433
+PL K +GG + + + W E E+ + V+S LQLS D LP K
Sbjct: 370 LPLTIKAVGGLLLCKDHVYHEWRRIAEHFQDELRGNTSETDNVMSSLQLSYDELPSHLKS 429
Query: 434 CFAYCSIFPKDCEVVKENLIRMWIAQGFIQPTEGENTMEDLGEGHFNFLLSRSLFQDVVK 493
C S++P+DC + K+ L+ WI +GF+ G + E GE F+ L +R L + V K
Sbjct: 430 CILTLSLYPEDCVIPKQQLVHGWIGEGFVMWRNGRSATES-GEDCFSGLTNRCLIEVVDK 489
Query: 494 DKYGRITHFKMHDLIHDVALAILSTRQKSVLDPTHWNG--------------KTSRKLRT 553
G I K+HD++ D+ + I ++ S +P N K + KLR
Sbjct: 490 TYSGTIITCKIHDMVRDLVIDI--AKKDSFSNPEGLNCRHLGISGNFDEKQIKVNHKLRG 549
Query: 554 LLYN---------NQEIHHKVADCVFLRVLEVNSLHM---MNNLPDFIAKLKHLRYLDIS 613
++ N ++ K DC +LRVL+++ ++ + D IA L+HL L +S
Sbjct: 550 VVSTTKTGEVNKLNSDLAKKFTDCKYLRVLDISKSIFDAPLSEILDEIASLQHLACLSLS 609
Query: 614 SCSMWV-MPHSVTTLFNLQTLKLGSIENLPMNLRNLVRLRHLEFHVYYNTRKM---PSHM 673
+ + P S+ L NLQ L +NL +V + L N + P +
Sbjct: 610 NTHPLIQFPRSMEDLHNLQILDASYCQNLKQLQPCIVLFKKLLVLDMTNCGSLECFPKGI 669
Query: 674 GELIHLQTLSWF-VAGFEEGCKIEELGNLKNLKGQLQLSNLEQVRSKEEALAAKLVNKKN 733
G L+ L+ L F A GCK+ E+ NL NL+ +L LS + +EE L + L+N
Sbjct: 670 GSLVKLEVLLGFKPARSNNGCKLSEVKNLTNLR-KLGLSLTRGDQIEEEELDS-LINLSK 729
Query: 734 LRELTFEWSIDILRECSSYND--FEVLEGLQPPKNLSSLKITNFGGKFLPA 743
L ++ + SY D ++ L PP L L + + GK P+
Sbjct: 730 LMSIS-------INCYDSYGDDLITKIDALTPPHQLHELSLQFYPGKSSPS 756
BLAST of CSPI04G03520 vs. TAIR10
Match:
AT5G43470.1 (AT5G43470.1 Disease resistance protein (CC-NBS-LRR class) family)
HSP 1 Score: 205.3 bits (521), Expect = 1.8e-52
Identity = 240/926 (25.92%), Postives = 421/926 (45.46%), Query Frame = 1
Query: 1 MAEFLWTFAVEETLKRTVKVAAQKISLVWGLEDELSNLSKWLLDAGALLRDIDREILRKE 60
MAE +F +E+ + +++ + G++ +L L + L +LL+D D + +
Sbjct: 1 MAEAFVSFGLEKLWD----LLSRESERLQGIDGQLDGLKRQLRSLQSLLKDADAKKHGSD 60
Query: 61 SVKRWADGLEDIVSEAEDLLDELAYEDLRRKVETSSRVCNNFKFSSVLNPLVRHDMACKM 120
V+ + + ++D+V +AED+++ L K + + + + L RH +A +
Sbjct: 61 RVRNFLEDVKDLVFDAEDIIESYVLNKLSGKGKGVKKHVR--RLACFLTD--RHKVASDI 120
Query: 121 KKITKMLKQHYRNSAPLGLV----GKESMEKEDGGNNLRQIRET-TSILNFDVVGRETEV 180
+ ITK + + G+ G S+ ++ R+IR+T D+VG E V
Sbjct: 121 EGITKRISEVIGEMQSFGIQQIIDGGRSLSLQERQRVQREIRQTYPDSSESDLVGVEQSV 180
Query: 181 LDILRLVIDSSSNEYELPLLIVPIVGMGGVGKTTLAKLVFRHELIKKHFHETIWICVSEH 240
+++ ++++ ++ +V I GMGG+GKTTLA+ VF H+L+++HF W+CVS+
Sbjct: 181 KELVGHLVENDVHQ------VVSIAGMGGIGKTTLARQVFHHDLVRRHFDGFAWVCVSQQ 240
Query: 241 FNIDEILVAILESLT----DKVPTKREAVLRRLQKELLDKRCFLVLDDVWNESSKLWEEL 300
F + IL+ L D + A+ R+L + L R +VLDDVW + W+ +
Sbjct: 241 FTQKHVWQRILQELQPHDGDILQMDEYALQRKLFQLLEAGRYLVVLDDVWKKED--WDVI 300
Query: 301 EDCLKEIVGKFGITIIVTTRLDEVA-DIMGTVSGYRLEKLPEDHCWSLFKR----SANAN 360
+ K G +++T+R + V T +R L + W L +R +
Sbjct: 301 KAVFPR---KRGWKMLLTSRNEGVGIHADPTCLTFRASILNPEESWKLCERIVFPRRDET 360
Query: 361 GVKMTPKLEAIRIKLLQKIDGIPLVAKVLGGAVEFEGDLDRWETTLESIVREI------P 420
V++ ++EA+ +++ G+PL K LGG + + + W+ ++I +I
Sbjct: 361 EVRLDEEMEAMGKEMVTHCGGLPLAVKALGGLLANKHTVPEWKRVFDNIGSQIVGGSWLD 420
Query: 421 MKQKSYVLSILQLSVDRLPFVEKQCFAYCSIFPKDCEVVKENLIRMWIAQGFIQPTEGEN 480
+ V IL LS + LP K CF + FP+D E+ +L W A+G +
Sbjct: 421 DNSLNSVYRILSLSYEDLPTHLKHCFLNLAHFPEDSEISTYSLFYYWAAEGIYD----GS 480
Query: 481 TMEDLGEGHFNFLLSRSLFQDVVKDKY--GRITHFKMHDLIHDVALAILSTRQ--KSVLD 540
T+ED GE + L+ R+L + D Y + + +MHD++ +V L+ + ++D
Sbjct: 481 TIEDSGEYYLEELVRRNLV--IADDNYLSWQSKYCQMHDMMREVCLSKAKEENFLQIIID 540
Query: 541 PTHWN----GKTSRKLRTLLYNNQEIH-------HKVADCVFLRVLE---VNSLHMMNNL 600
PT + SR R +++ + H KV + R E + S + +N
Sbjct: 541 PTCTSTINAQSPSRSRRLSIHSGKAFHILGHKNKTKVRSLIVPRFEEDYWIRSASVFHN- 600
Query: 601 PDFIAKLKHLRYLDISSCSMWV------MPHSVTTLFNLQTLKL--GSIENLPMNLRNLV 660
L LR LD+S WV +P S+ L +L+ L L + +LP +RNL
Sbjct: 601 ------LTLLRVLDLS----WVKFEGGKLPCSIGGLIHLRYLSLYEAKVSHLPSTMRNLK 660
Query: 661 RLRHLEFHVYYNTRK---MPSHMGELIHLQTLSWFVAGFEEGCKIE--ELGNLKNLKG-Q 720
L +L V +T + +P+ + E+I L+ LS ++ K+E +L NL+ L G
Sbjct: 661 LLLYLNLRV--DTEEPIHVPNVLKEMIQLRYLS-LPLKMDDKTKLELGDLVNLEYLYGFS 720
Query: 721 LQLSNLEQV--RSKEEALAAKLVNKKN-------LRELTFEWSIDILRECSSYN-DFEVL 780
Q S++ + +K LA L + N LREL +++ L +Y D+
Sbjct: 721 TQHSSVTDLLRMTKLRYLAVSLSERCNFETLSSSLRELRNLETLNFLFSLETYMVDYMGE 780
Query: 781 EGLQPPKNLSSLKITNFGGKFLPAATFVENLVFLCLYGC-TKCERLPMLGQLANLQELSI 840
L +L L + K F +LV L L C + + +P+L +L +L+
Sbjct: 781 FVLDHFIHLKQLGLAVRMSKIPDQHQFPPHLVHLFLIYCGMEEDPMPILEKLLHLK---- 840
Query: 841 CFMDSVRSIGSEFYGIDS-NRRGYFPKLKKFDFCWMCNLEQWELEVANHESNHFGSLQTL 863
SVR F G +G FP+L + LE+W + E L+TL
Sbjct: 841 ----SVRLARKAFLGSRMVCSKGGFPQLCVIEISKESELEEWIV-----EEGSMPCLRTL 874
BLAST of CSPI04G03520 vs. NCBI nr
Match:
gi|449469162|ref|XP_004152290.1| (PREDICTED: disease resistance protein RGA2-like [Cucumis sativus])
HSP 1 Score: 2100.9 bits (5442), Expect = 0.0e+00
Identity = 1042/1045 (99.71%), Postives = 1043/1045 (99.81%), Query Frame = 1
Query: 1 MAEFLWTFAVEETLKRTVKVAAQKISLVWGLEDELSNLSKWLLDAGALLRDIDREILRKE 60
MAEFLWTFAVEETLKRTV VAAQKISLVWGLEDELSNLSKWLLDAGALLRDIDREILRKE
Sbjct: 1 MAEFLWTFAVEETLKRTVNVAAQKISLVWGLEDELSNLSKWLLDAGALLRDIDREILRKE 60
Query: 61 SVKRWADGLEDIVSEAEDLLDELAYEDLRRKVETSSRVCNNFKFSSVLNPLVRHDMACKM 120
SVKRWADGLEDIVSEAEDLLDELAYEDLRRKVETSSRVCNNFKFSSVLNPLVRHDMACKM
Sbjct: 61 SVKRWADGLEDIVSEAEDLLDELAYEDLRRKVETSSRVCNNFKFSSVLNPLVRHDMACKM 120
Query: 121 KKITKMLKQHYRNSAPLGLVGKESMEKEDGGNNLRQIRETTSILNFDVVGRETEVLDILR 180
KKITKMLKQHYRNSAPLGLVGKESMEKEDGGNNLRQIRETTSILNFDVVGRETEVLDILR
Sbjct: 121 KKITKMLKQHYRNSAPLGLVGKESMEKEDGGNNLRQIRETTSILNFDVVGRETEVLDILR 180
Query: 181 LVIDSSSNEYELPLLIVPIVGMGGVGKTTLAKLVFRHELIKKHFHETIWICVSEHFNIDE 240
LVIDSSSNEYELPLLIVPIVGMGGVGKTTLAKLVFRHELIKKHFHETIWICVSEHFNIDE
Sbjct: 181 LVIDSSSNEYELPLLIVPIVGMGGVGKTTLAKLVFRHELIKKHFHETIWICVSEHFNIDE 240
Query: 241 ILVAILESLTDKVPTKREAVLRRLQKELLDKRCFLVLDDVWNESSKLWEELEDCLKEIVG 300
ILVAILESLTDKVPTKREAVLRRLQKELLDKRCFLVLDDVWNESSKLWEELEDCLKEIVG
Sbjct: 241 ILVAILESLTDKVPTKREAVLRRLQKELLDKRCFLVLDDVWNESSKLWEELEDCLKEIVG 300
Query: 301 KFGITIIVTTRLDEVADIMGTVSGYRLEKLPEDHCWSLFKRSANANGVKMTPKLEAIRIK 360
KFGITIIVTTRLDEVA+IMGTVSGYRLEKLPEDHCWSLFKRSANANGVKMTPKLEAIRIK
Sbjct: 301 KFGITIIVTTRLDEVANIMGTVSGYRLEKLPEDHCWSLFKRSANANGVKMTPKLEAIRIK 360
Query: 361 LLQKIDGIPLVAKVLGGAVEFEGDLDRWETTLESIVREIPMKQKSYVLSILQLSVDRLPF 420
LLQKIDGIPLVAKVLGGAVEFEGDLDRWETTLESIVREIPMKQKSYVLSILQLSVDRLPF
Sbjct: 361 LLQKIDGIPLVAKVLGGAVEFEGDLDRWETTLESIVREIPMKQKSYVLSILQLSVDRLPF 420
Query: 421 VEKQCFAYCSIFPKDCEVVKENLIRMWIAQGFIQPTEGENTMEDLGEGHFNFLLSRSLFQ 480
VEKQCFAYCSIFPKDCEVVKENLIRMWIAQGFIQPTEGENTMEDLGEGHFNFLLSRSLFQ
Sbjct: 421 VEKQCFAYCSIFPKDCEVVKENLIRMWIAQGFIQPTEGENTMEDLGEGHFNFLLSRSLFQ 480
Query: 481 DVVKDKYGRITHFKMHDLIHDVALAILSTRQKSVLDPTHWNGKTSRKLRTLLYNNQEIHH 540
DVVKDKYGRITHFKMHDLIHDVALAILSTRQKSVLDPTHWNGKTSRKLRTLLYNNQEIHH
Sbjct: 481 DVVKDKYGRITHFKMHDLIHDVALAILSTRQKSVLDPTHWNGKTSRKLRTLLYNNQEIHH 540
Query: 541 KVADCVFLRVLEVNSLHMMNNLPDFIAKLKHLRYLDISSCSMWVMPHSVTTLFNLQTLKL 600
KVADCVFLRVLEVNSLHMMNNLPDFIAKLKHLRYLDISSCSMWVMPHSVTTLFNLQTLKL
Sbjct: 541 KVADCVFLRVLEVNSLHMMNNLPDFIAKLKHLRYLDISSCSMWVMPHSVTTLFNLQTLKL 600
Query: 601 GSIENLPMNLRNLVRLRHLEFHVYYNTRKMPSHMGELIHLQTLSWFVAGFEEGCKIEELG 660
GSIENLPMNLRNLVRLRHLEFHVYYNTRKMPSHMGELIHLQ LSWFVAGFEEGCKIEELG
Sbjct: 601 GSIENLPMNLRNLVRLRHLEFHVYYNTRKMPSHMGELIHLQILSWFVAGFEEGCKIEELG 660
Query: 661 NLKNLKGQLQLSNLEQVRSKEEALAAKLVNKKNLRELTFEWSIDILRECSSYNDFEVLEG 720
NLKNLKGQLQLSNLEQVRSKEEALAAKLVNKKNLRELTFEWSIDILRECSSYNDFEVLEG
Sbjct: 661 NLKNLKGQLQLSNLEQVRSKEEALAAKLVNKKNLRELTFEWSIDILRECSSYNDFEVLEG 720
Query: 721 LQPPKNLSSLKITNFGGKFLPAATFVENLVFLCLYGCTKCERLPMLGQLANLQELSICFM 780
LQPPKNLSSLKITNFGGKFLPAATFVENLVFLCLYGCTKCERLPMLGQLANLQELSICFM
Sbjct: 721 LQPPKNLSSLKITNFGGKFLPAATFVENLVFLCLYGCTKCERLPMLGQLANLQELSICFM 780
Query: 781 DSVRSIGSEFYGIDSNRRGYFPKLKKFDFCWMCNLEQWELEVANHESNHFGSLQTLKLDR 840
DSVRSIGSEFYGIDSNRRGYFPKLKKFDFCWMCNLEQWELEVANHESNHFGSLQTLKLDR
Sbjct: 781 DSVRSIGSEFYGIDSNRRGYFPKLKKFDFCWMCNLEQWELEVANHESNHFGSLQTLKLDR 840
Query: 841 CGKLTKLPNGLECCKSVHEVIISNCPNLTLNVEEMHNLSVLLIDGLKFLPKGLALHPNLK 900
CGKLTKLPNGLECCKSVHEVIISNCPNLTLNVEEMHNLSVLLIDGLKFLPKGLALHPNLK
Sbjct: 841 CGKLTKLPNGLECCKSVHEVIISNCPNLTLNVEEMHNLSVLLIDGLKFLPKGLALHPNLK 900
Query: 901 TIMIKGCIEDYDYSPFLNLPSLTKLYLNDGLGNATQLPKQLQHLTALKILAIENFYGIEV 960
TIMIKGCIEDYDYSPFLNLPSLTKLYLNDGLGNATQLPKQLQHLTALKILAIENFYGIEV
Sbjct: 901 TIMIKGCIEDYDYSPFLNLPSLTKLYLNDGLGNATQLPKQLQHLTALKILAIENFYGIEV 960
Query: 961 LPEWLRKLTCLETLDLVRCKNLKRLPSRGAMRCLTKLKDFKVIACPLLLLGGQADQEGAK 1020
LPEWLRKLTCLETLDLVRCKNLKRLPSRGAMRCLTKLKDFKVIACPLLLLGGQADQEGAK
Sbjct: 961 LPEWLRKLTCLETLDLVRCKNLKRLPSRGAMRCLTKLKDFKVIACPLLLLGGQADQEGAK 1020
Query: 1021 YLHIPAYLCHVYQSRGSPLSKTSSI 1046
YLHIPAYLCHVYQSRGSPLSKTSSI
Sbjct: 1021 YLHIPAYLCHVYQSRGSPLSKTSSI 1045
BLAST of CSPI04G03520 vs. NCBI nr
Match:
gi|659108002|ref|XP_008453965.1| (PREDICTED: disease resistance protein RGA2-like [Cucumis melo])
HSP 1 Score: 1970.3 bits (5103), Expect = 0.0e+00
Identity = 976/1046 (93.31%), Postives = 1010/1046 (96.56%), Query Frame = 1
Query: 1 MAEFLWTFAVEETLKRTVKVAAQKISLVWGLEDELSNLSKWLLDAGALLRDIDREILRKE 60
MAEFLWTFAVEETLKRTVKVAAQKI++VWGLEDELSNLSKWLLDAGALLRDIDREILRKE
Sbjct: 1 MAEFLWTFAVEETLKRTVKVAAQKIAVVWGLEDELSNLSKWLLDAGALLRDIDREILRKE 60
Query: 61 SVKRWADGLEDIVSEAEDLLDELAYEDLRRKVETSSRVCNNFKFSSVLNPLVRHDMACKM 120
SVKRW DGLEDIVSEAEDLLDELAYEDLRRKVET+SRVCNNFKFSSVLNPLVRHDMACK+
Sbjct: 61 SVKRWVDGLEDIVSEAEDLLDELAYEDLRRKVETTSRVCNNFKFSSVLNPLVRHDMACKV 120
Query: 121 KKITKMLKQHYRNSAPLGLVGKESMEKEDGGNNLRQIRETTSILNFDVVGRETEVLDILR 180
K ITKMLKQHYR SAPLGLVGKESMEKEDGGNNLRQIRETTSILNFDVVGRE EVLDILR
Sbjct: 121 KTITKMLKQHYRKSAPLGLVGKESMEKEDGGNNLRQIRETTSILNFDVVGREAEVLDILR 180
Query: 181 LVIDSSSNEYELPLLIVPIVGMGGVGKTTLAKLVFRHELIKKHFHETIWICVSEHFNIDE 240
LVIDSSSNE ELPLLIVPIVGMGGVGKTTLAKLVFRHELIKKHFHETIW+CVSE+FNIDE
Sbjct: 181 LVIDSSSNESELPLLIVPIVGMGGVGKTTLAKLVFRHELIKKHFHETIWVCVSENFNIDE 240
Query: 241 ILVAILESLTDKVPTKREAVLRRLQKELLDKRCFLVLDDVWNESSKLWEELEDCLKEIVG 300
ILVAILESL+DKVPT+REA+LRRLQKEL DKRCFLVLDDVWNESSKLWEELEDCLKEIVG
Sbjct: 241 ILVAILESLSDKVPTRREALLRRLQKELQDKRCFLVLDDVWNESSKLWEELEDCLKEIVG 300
Query: 301 KFGITIIVTTRLDEVADIMGTVSGYRLEKLPEDHCWSLFKRSANANGVKMTPKLEAIRIK 360
KFGITI+VTTRLDEVA+IMGTVSGYRLEKLPEDHCWSLFKRSANANG+KMTPKLEAIRIK
Sbjct: 301 KFGITIMVTTRLDEVANIMGTVSGYRLEKLPEDHCWSLFKRSANANGIKMTPKLEAIRIK 360
Query: 361 LLQKIDGIPLVAKVLGGAVEFEGDLDRWETTLESIVREIPMKQKSYVLSILQLSVDRLPF 420
LLQKIDGIPLVAKVLGGAVEFEGDLDRWETTLES+VREIPMKQKSYVLSILQLSVDRLPF
Sbjct: 361 LLQKIDGIPLVAKVLGGAVEFEGDLDRWETTLESVVREIPMKQKSYVLSILQLSVDRLPF 420
Query: 421 VEKQCFAYCSIFPKDCEVVKENLIRMWIAQGFIQPTEGENTM-EDLGEGHFNFLLSRSLF 480
VEKQCFAYCSIFPKDCEVVKE+LIRMWIAQGFIQP EGEN M EDLGEG+FNFLLSRSLF
Sbjct: 421 VEKQCFAYCSIFPKDCEVVKESLIRMWIAQGFIQPPEGENMMMEDLGEGYFNFLLSRSLF 480
Query: 481 QDVVKDKYGRITHFKMHDLIHDVALAILSTRQKSVLDPTHWNGKTSRKLRTLLYNNQEIH 540
QDVVKDKYG ITHFKMHDLIHDVALAILS RQK VLDPTHWNGKT+RKLRTL+YNNQEIH
Sbjct: 481 QDVVKDKYGSITHFKMHDLIHDVALAILSNRQKMVLDPTHWNGKTARKLRTLIYNNQEIH 540
Query: 541 HKVADCVFLRVLEVNSLHMMNNLPDFIAKLKHLRYLDISSCSMWVMPHSVTTLFNLQTLK 600
HK+ADCVFLRVLEVNSL MMNNLPDFIAKLKHLRYLDISSC MW +PHS+TTLFNLQTLK
Sbjct: 541 HKLADCVFLRVLEVNSLRMMNNLPDFIAKLKHLRYLDISSCPMWAIPHSITTLFNLQTLK 600
Query: 601 LGSIENLPMNLRNLVRLRHLEFHVYYNTRKMPSHMGELIHLQTLSWFVAGFEEGCKIEEL 660
LGSIENLPMNLRNLVRLRHLEFHVYYNTRKMP HMGELIHLQTLS FVAGFEEGCKIEEL
Sbjct: 601 LGSIENLPMNLRNLVRLRHLEFHVYYNTRKMPPHMGELIHLQTLSGFVAGFEEGCKIEEL 660
Query: 661 GNLKNLKGQLQLSNLEQVRSKEEALAAKLVNKKNLRELTFEWSIDILRECSSYNDFEVLE 720
GNLKNL+G+LQLSNLEQVRSK+EA+A KLV+KKNLRELTFEWSID+LRE SSYNDFEVLE
Sbjct: 661 GNLKNLRGKLQLSNLEQVRSKQEAIAVKLVDKKNLRELTFEWSIDLLREFSSYNDFEVLE 720
Query: 721 GLQPPKNLSSLKITNFGGKFLPAATFVENLVFLCLYGCTKCERLPMLGQLANLQELSICF 780
GLQPPKNL+SLKITNFGGKFLPA TFVENL FLCLYGCTKCERLPMLGQL NLQEL ICF
Sbjct: 721 GLQPPKNLTSLKITNFGGKFLPAGTFVENLAFLCLYGCTKCERLPMLGQLPNLQELGICF 780
Query: 781 MDSVRSIGSEFYGIDSNRRGYFPKLKKFDFCWMCNLEQWELEVANHESNHFGSLQTLKLD 840
+D V SIG+EFYG DSNRRGYFPKLKKFD WMCNLEQWELEVANHESNHFGSLQTLKLD
Sbjct: 781 LDRVISIGNEFYGNDSNRRGYFPKLKKFDLGWMCNLEQWELEVANHESNHFGSLQTLKLD 840
Query: 841 RCGKLTKLPNGLECCKSVHEVIISNCPNLTLNVEEMHNLSVLLIDGLKFLPKGLALHPNL 900
RCGKLTKLPNGLECCKSV EVIISNCPNLTLNVE+MH+LSVLLIDGLKFLPKGLA PNL
Sbjct: 841 RCGKLTKLPNGLECCKSVREVIISNCPNLTLNVEKMHSLSVLLIDGLKFLPKGLAHLPNL 900
Query: 901 KTIMIKGCIEDYDYSPFLNLPSLTKLYLNDGLGNATQLPKQLQHLTALKILAIENFYGIE 960
KT+MI GCIEDYDYSPFLNLPSLTKLYLNDGLGNATQLP+QLQHLTALKILAIENFYGIE
Sbjct: 901 KTMMITGCIEDYDYSPFLNLPSLTKLYLNDGLGNATQLPQQLQHLTALKILAIENFYGIE 960
Query: 961 VLPEWLRKLTCLETLDLVRCKNLKRLPSRGAMRCLTKLKDFKVIACPLLLLGGQADQEGA 1020
VLPEWLRKLTCLETLDLVRCKNLKRLPSR AM CLTKLKDFKV+ACPLLLLGGQADQEGA
Sbjct: 961 VLPEWLRKLTCLETLDLVRCKNLKRLPSREAMLCLTKLKDFKVMACPLLLLGGQADQEGA 1020
Query: 1021 KYLHIPAYLCHVYQSRGSPLSKTSSI 1046
KYLHIPAYLCHVYQSRGS LS TSSI
Sbjct: 1021 KYLHIPAYLCHVYQSRGSLLSNTSSI 1046
BLAST of CSPI04G03520 vs. NCBI nr
Match:
gi|700197939|gb|KGN53097.1| (hypothetical protein Csa_4G016360 [Cucumis sativus])
HSP 1 Score: 1549.3 bits (4010), Expect = 0.0e+00
Identity = 757/759 (99.74%), Postives = 758/759 (99.87%), Query Frame = 1
Query: 287 LWEELEDCLKEIVGKFGITIIVTTRLDEVADIMGTVSGYRLEKLPEDHCWSLFKRSANAN 346
LWEELEDCLKEIVGKFGITIIVTTRLDEVA+IMGTVSGYRLEKLPEDHCWSLFKRSANAN
Sbjct: 200 LWEELEDCLKEIVGKFGITIIVTTRLDEVANIMGTVSGYRLEKLPEDHCWSLFKRSANAN 259
Query: 347 GVKMTPKLEAIRIKLLQKIDGIPLVAKVLGGAVEFEGDLDRWETTLESIVREIPMKQKSY 406
GVKMTPKLEAIRIKLLQKIDGIPLVAKVLGGAVEFEGDLDRWETTLESIVREIPMKQKSY
Sbjct: 260 GVKMTPKLEAIRIKLLQKIDGIPLVAKVLGGAVEFEGDLDRWETTLESIVREIPMKQKSY 319
Query: 407 VLSILQLSVDRLPFVEKQCFAYCSIFPKDCEVVKENLIRMWIAQGFIQPTEGENTMEDLG 466
VLSILQLSVDRLPFVEKQCFAYCSIFPKDCEVVKENLIRMWIAQGFIQPTEGENTMEDLG
Sbjct: 320 VLSILQLSVDRLPFVEKQCFAYCSIFPKDCEVVKENLIRMWIAQGFIQPTEGENTMEDLG 379
Query: 467 EGHFNFLLSRSLFQDVVKDKYGRITHFKMHDLIHDVALAILSTRQKSVLDPTHWNGKTSR 526
EGHFNFLLSRSLFQDVVKDKYGRITHFKMHDLIHDVALAILSTRQKSVLDPTHWNGKTSR
Sbjct: 380 EGHFNFLLSRSLFQDVVKDKYGRITHFKMHDLIHDVALAILSTRQKSVLDPTHWNGKTSR 439
Query: 527 KLRTLLYNNQEIHHKVADCVFLRVLEVNSLHMMNNLPDFIAKLKHLRYLDISSCSMWVMP 586
KLRTLLYNNQEIHHKVADCVFLRVLEVNSLHMMNNLPDFIAKLKHLRYLDISSCSMWVMP
Sbjct: 440 KLRTLLYNNQEIHHKVADCVFLRVLEVNSLHMMNNLPDFIAKLKHLRYLDISSCSMWVMP 499
Query: 587 HSVTTLFNLQTLKLGSIENLPMNLRNLVRLRHLEFHVYYNTRKMPSHMGELIHLQTLSWF 646
HSVTTLFNLQTLKLGSIENLPMNLRNLVRLRHLEFHVYYNTRKMPSHMGELIHLQ LSWF
Sbjct: 500 HSVTTLFNLQTLKLGSIENLPMNLRNLVRLRHLEFHVYYNTRKMPSHMGELIHLQILSWF 559
Query: 647 VAGFEEGCKIEELGNLKNLKGQLQLSNLEQVRSKEEALAAKLVNKKNLRELTFEWSIDIL 706
VAGFEEGCKIEELGNLKNLKGQLQLSNLEQVRSKEEALAAKLVNKKNLRELTFEWSIDIL
Sbjct: 560 VAGFEEGCKIEELGNLKNLKGQLQLSNLEQVRSKEEALAAKLVNKKNLRELTFEWSIDIL 619
Query: 707 RECSSYNDFEVLEGLQPPKNLSSLKITNFGGKFLPAATFVENLVFLCLYGCTKCERLPML 766
RECSSYNDFEVLEGLQPPKNLSSLKITNFGGKFLPAATFVENLVFLCLYGCTKCERLPML
Sbjct: 620 RECSSYNDFEVLEGLQPPKNLSSLKITNFGGKFLPAATFVENLVFLCLYGCTKCERLPML 679
Query: 767 GQLANLQELSICFMDSVRSIGSEFYGIDSNRRGYFPKLKKFDFCWMCNLEQWELEVANHE 826
GQLANLQELSICFMDSVRSIGSEFYGIDSNRRGYFPKLKKFDFCWMCNLEQWELEVANHE
Sbjct: 680 GQLANLQELSICFMDSVRSIGSEFYGIDSNRRGYFPKLKKFDFCWMCNLEQWELEVANHE 739
Query: 827 SNHFGSLQTLKLDRCGKLTKLPNGLECCKSVHEVIISNCPNLTLNVEEMHNLSVLLIDGL 886
SNHFGSLQTLKLDRCGKLTKLPNGLECCKSVHEVIISNCPNLTLNVEEMHNLSVLLIDGL
Sbjct: 740 SNHFGSLQTLKLDRCGKLTKLPNGLECCKSVHEVIISNCPNLTLNVEEMHNLSVLLIDGL 799
Query: 887 KFLPKGLALHPNLKTIMIKGCIEDYDYSPFLNLPSLTKLYLNDGLGNATQLPKQLQHLTA 946
KFLPKGLALHPNLKTIMIKGCIEDYDYSPFLNLPSLTKLYLNDGLGNATQLPKQLQHLTA
Sbjct: 800 KFLPKGLALHPNLKTIMIKGCIEDYDYSPFLNLPSLTKLYLNDGLGNATQLPKQLQHLTA 859
Query: 947 LKILAIENFYGIEVLPEWLRKLTCLETLDLVRCKNLKRLPSRGAMRCLTKLKDFKVIACP 1006
LKILAIENFYGIEVLPEWLRKLTCLETLDLVRCKNLKRLPSRGAMRCLTKLKDFKVIACP
Sbjct: 860 LKILAIENFYGIEVLPEWLRKLTCLETLDLVRCKNLKRLPSRGAMRCLTKLKDFKVIACP 919
Query: 1007 LLLLGGQADQEGAKYLHIPAYLCHVYQSRGSPLSKTSSI 1046
LLLLGGQADQEGAKYLHIPAYLCHVYQSRGSPLSKTSSI
Sbjct: 920 LLLLGGQADQEGAKYLHIPAYLCHVYQSRGSPLSKTSSI 958
BLAST of CSPI04G03520 vs. NCBI nr
Match:
gi|449445363|ref|XP_004140442.1| (PREDICTED: disease resistance protein RGA2-like [Cucumis sativus])
HSP 1 Score: 980.7 bits (2534), Expect = 2.0e-282
Identity = 554/1048 (52.86%), Postives = 720/1048 (68.70%), Query Frame = 1
Query: 1 MAEFLWTFAVEETLKRTVKVAAQKISLVWGLEDELSNLSKWLLDAGALLRDIDREILRKE 60
MAEFLWTFAV+E LK+ V A++ISL WGLE ELS+L KWLL A +L DI+ +
Sbjct: 1 MAEFLWTFAVQEVLKKIVNFGAEQISLAWGLEKELSHLKKWLLKAQTILADINTKKSHHH 60
Query: 61 SVKRWADGLEDIVSEAEDLLDELAYEDLRRKVETSSRVCNNFKFSSVLNP-----LVRHD 120
SV W + L DI+ EA+DLLDE+ YE +R+ VE + ++ K ++P L
Sbjct: 61 SVGLWVEELHDIIYEADDLLDEIVYEQIRQTVEQTGKLR---KVRDSISPSKNSFLFGLK 120
Query: 121 MACKMKKITKMLKQHYRNSAPLGLVGKESMEKEDGGNNLRQIRETTSILNFDVVGRETEV 180
MA KMKKITK L +HY ++PLGLVG ES + + L QIRETTSIL+F+V GRE EV
Sbjct: 121 MAKKMKKITKTLYEHYCEASPLGLVGDESTTESEAA--LNQIRETTSILDFEVEGREAEV 180
Query: 181 LDILRLVIDSSSNEYELPLLIVPIVGMGGVGKTTLAKLVFRHELIKKHFHETIWICVSEH 240
L+IL+LVIDS+ ++ + ++ IVGMGG+GKTTLAK+VF H+ IK HF +T+W+CVS+
Sbjct: 181 LEILKLVIDSTDEDH---ISVISIVGMGGLGKTTLAKMVFNHDAIKGHFDKTVWVCVSKP 240
Query: 241 FNIDEILVAILESLTDKVP--TKREAVLRRLQKELLDKRCFLVLDDVWNESSKLWEELED 300
F + +IL AI + LT+ REA+L RL++E+ K+ FLVLDDVW++ + LW+EL
Sbjct: 241 FIVMKILEAIFQGLTNTSSGLNSREALLNRLREEMQGKKYFLVLDDVWDKENCLWDELIG 300
Query: 301 CLKEIVGKFGITIIVTTRLDEVADIMGTVSGYRLEKLPEDHCWSLFKRSANANGVKMTPK 360
LK I GK G +I+VTTR EVA ++ TV Y L+KL +DHCW+L K+SANAN ++M K
Sbjct: 301 NLKYIAGKSGNSIMVTTRSVEVATMVKTVPIYHLKKLSDDHCWALLKKSANANQLQMNSK 360
Query: 361 LEAIRIKLLQKIDGIPLVAKVLGGAVEFE-GDLDRWETTLESIVREIPMKQKSYVLSILQ 420
LE + L++KI G+PL+AKVLGGAV+FE G + W +ES R I ++ K +VLSIL+
Sbjct: 361 LENTKNILVRKIGGVPLIAKVLGGAVKFEEGGSESWMAKIESFARNISIEDKDFVLSILK 420
Query: 421 LSVDRLPFVE-KQCFAYCSIFPKDCEVVKENLIRMWIAQGFIQPT-EGEN-TMEDLGEGH 480
LSV+ LP KQCFAYCS FP+D E K+ I+MWIA+GFIQP E EN TME++GE +
Sbjct: 421 LSVESLPHSALKQCFAYCSNFPQDYEFDKDEAIQMWIAEGFIQPEQERENLTMENIGEEY 480
Query: 481 FNFLLSRSLFQDVVKDKYGRITHFKMHDLIHDVALAILSTRQKSVLDPTHWNGKTSRKLR 540
NFLLSRSLF+D +K GRI FK+HDL+HD+A AI S K +P WNGK++RKLR
Sbjct: 481 LNFLLSRSLFEDAIKYD-GRIVTFKIHDLMHDIACAI-SNHHKMDSNPISWNGKSTRKLR 540
Query: 541 TLLYNNQEIHHKV-ADCVFLRVLEVNSLHMMNNLPDFIAKLKHLRYLDISSCSM-WVMPH 600
TL+ N+E HK+ D + LRVL + N L + KL HLRYLDIS+C++ ++
Sbjct: 541 TLICENEEAFHKIQTDIICLRVLVLKWFDT-NTLSTIMDKLIHLRYLDISNCNINKLLRD 600
Query: 601 SVTTLFNLQTLKLGSIE-NLPMNLRNLVRLRHLEFHVYYNTRKMPSHMGELIHLQTLSWF 660
S+ L+NLQTLKLG IE +LP NLRNLV LRHLEF +++ +MPSHMG +IHLQTLS F
Sbjct: 601 SICALYNLQTLKLGYIECDLPKNLRNLVNLRHLEFKKFFDMGQMPSHMGNMIHLQTLSEF 660
Query: 661 VAGFEEGCKIEELGNLKNLKGQLQLSNLEQVRSKEEALAAKLVNKKNLRELTFEWSIDIL 720
V G E+GCKI+ELG LK+LKG L L NL+ V++K+EA+AAKLV KK LR L F+W +++
Sbjct: 661 VVGLEKGCKIDELGPLKDLKGTLTLKNLQNVQNKDEAMAAKLVEKKYLRHLIFQWFLNLY 720
Query: 721 R--ECSSYNDFEVLEGLQPPKNLSSLKITNFGGKFLPAATFVENLVFLCLYGCTKCERLP 780
E ++ +VLEGLQP KN+ SL I F G+ L FVENLV + L C +CE LP
Sbjct: 721 DRGEYDEDDNKQVLEGLQPHKNVQSLDIRGFQGRVLNNNIFVENLVEIRLVDCGRCEVLP 780
Query: 781 MLGQLANLQELSICFMDSVRSIGSEFYGIDSNRRGY--FPKLKKFDFCWMCNLEQWELEV 840
MLGQL NL++L I M+SVRSIGSEFYG+D N R FP+L KF C + L+QW+ E
Sbjct: 781 MLGQLPNLKKLEIISMNSVRSIGSEFYGVDCNDRNSSAFPQLNKFHICGLKKLQQWD-EA 840
Query: 841 ANHESNHFGSLQTLKLDRCGKLTKLPNGLECCKSVHEVIISNCPNLTLNVEEMHNLSVLL 900
SN FG L+ L L C +L KLP+GLE C S+ + I CPNL LNV+ ++NL L
Sbjct: 841 TVFASNRFGCLKELILSGCHQLAKLPSGLEGCYSIEYLAIDGCPNLMLNVQNLYNLYHLD 900
Query: 901 IDGLKFLPKGLALHPNLKTIMIKGCIEDYDYSPFLNLPS-LTKLYLNDGLGNA---TQLP 960
I GLK LP NLK + I GC+++Y++SPF++L S L +L L D + TQLP
Sbjct: 901 IRGLKRLPDEFGKLTNLKKLRIGGCMQNYEFSPFIHLSSQLVELELTDDGSSGSETTQLP 960
Query: 961 KQLQHLTALKILAIENFYGIEVLPEWLRKLTCLETLDLVRCKNLKRLPSRGAMRCLTKLK 1020
+QLQHLT LK+L I +F IEVLPEWL LTCL TL + CKNLK LPSR A++ LTKL
Sbjct: 961 QQLQHLTNLKVLKIADFDDIEVLPEWLGNLTCLATLVFLECKNLKELPSREAIQRLTKLD 1020
Query: 1021 DFKVIACPLLLLGGQADQEGAKYLHIPA 1027
D + CP LLL G+ DQE AK H+P+
Sbjct: 1021 DLVIDGCPKLLL-GEGDQERAKLSHLPS 1035
BLAST of CSPI04G03520 vs. NCBI nr
Match:
gi|778685319|ref|XP_004136783.2| (PREDICTED: disease resistance protein RGA2-like [Cucumis sativus])
HSP 1 Score: 927.5 bits (2396), Expect = 2.0e-266
Identity = 547/1060 (51.60%), Postives = 717/1060 (67.64%), Query Frame = 1
Query: 1 MAEFLWTFAVEETLKRTVKVAAQKISLVWGLEDELSNLSKWLLDAGALLRDIDREILRK- 60
MAE + TF V+E LKR VK A++I + W LE+E+S L L DA +L DI+R+
Sbjct: 1 MAE-IGTFVVQEVLKRIVKYGAEQIVVAWELENEVSLLKDKLHDADTILEDINRKKSHPG 60
Query: 61 ESVKRWADGLEDIVSEAEDLLDELAYEDLRRKVETS---SRVCNNFKFSSVLNPLVRHDM 120
SVKRW + LEDIV EA+DLLDEL YE LRR VE + S+V ++ SS+ + L R M
Sbjct: 61 NSVKRWVEKLEDIVHEADDLLDELVYEHLRRTVEHTEKFSKVSDSIS-SSINSFLFRRKM 120
Query: 121 ACKMKKITKMLKQHYRNSAPLGLVGKESMEKEDGGNNLRQIRETTSILNFDVVGRETEVL 180
A K+K IT L QHY ++ GLVG E++ + + L QIRETTSIL+F V GRE EVL
Sbjct: 121 AKKIKNITDTLNQHYCAASAFGLVGVETVTEIELA--LNQIRETTSILDFQVEGREAEVL 180
Query: 181 DILRLVIDSSSNEYELPLLIVPIVGMGGVGKTTLAKLVFRHELIKKHFHETIWICVSEHF 240
++L+L IDS++ + + ++ IVGMGG+GKTTLAK++F H I+ HF +TIW+CVS+ F
Sbjct: 181 ELLKLAIDSTNEHH---MSVISIVGMGGLGKTTLAKMIFNHREIEGHFDKTIWVCVSKPF 240
Query: 241 NIDEILVAILESLTDK---VPTKREAVLRRLQKELLDKRCFLVLDDVWNESSKLWEELED 300
+ +IL I + LT + + +EA+L RL+KE+ DK FLVLDDVW+ LW+EL
Sbjct: 241 IVTKILEKIFQGLTKTCSGLESNKEALLGRLRKEMQDKNYFLVLDDVWDNEKHLWDELRG 300
Query: 301 CLKEIVGKFGITIIVTTRLDEVADIMGTVSGYRLEKLPEDHCWSLFKRSANANGVKMTPK 360
CLK I GK G TI+VTTR +EVA ++ +S YRL+KL D CW+LFK SANAN + M K
Sbjct: 301 CLKHIAGKPGNTIMVTTRNEEVATMVEPISIYRLKKLSNDQCWALFKESANANQLPMNSK 360
Query: 361 LEAIRIKLLQKIDGIPLVAKVLGGAVEF-EGDLDR------WETTLESIVREIPMKQKSY 420
LE ++ +L++K+ G+PLVAKVLGGAV+F E +L+ W T +ESIVR I ++ K +
Sbjct: 361 LEIMKKELVRKMGGVPLVAKVLGGAVKFEETELEEEDHEISWMTKVESIVRNISLEDKDF 420
Query: 421 VLSILQLSVDRLPF-VEKQCFAYCSIFPKDCEVVKENLIRMWIAQGFIQPTEGEN---TM 480
VLSIL+LSVD LP V KQC AYCS F +D + K++LI+MWIAQGFIQP +G + M
Sbjct: 421 VLSILKLSVDSLPNPVLKQCVAYCSNFSQDYDFQKDDLIKMWIAQGFIQPGQGRDKNLLM 480
Query: 481 EDLGEGHFNFLLSRSLFQDVVKDKYGRITHFKMHDLIHDVALAILSTRQKSVLDPTHWNG 540
ED+GE +FNFLLSRS+FQDV +D RI FKMHDL+HD+A AI S+ Q +P + +G
Sbjct: 481 EDIGEQYFNFLLSRSIFQDVTRDANKRIVGFKMHDLMHDIACAI-SSHQNVESNPNNLSG 540
Query: 541 KTSRKLRTLLYNNQEIHH-KVADCVFLRVLEVNSLHMMNNLPDFIAKLKHLRYLDISSCS 600
K+ RKLRTL+ N++ I++ D V LRVL+V +L I KL HLRYLDIS CS
Sbjct: 541 KSVRKLRTLICNDEVINYLNQNDIVCLRVLKV-IFQSHTDLWIPIDKLIHLRYLDISECS 600
Query: 601 M-WVMPHSVTTLFNLQTLKLGSIENLPMNLRNLVRLRHLEFHVYYNTRKMPSHMGELIHL 660
+ ++ S++ L+NLQTLKLG LP NLR LV LRHLEF ++ +T MPS MG LIHL
Sbjct: 601 INKLLLESLSLLYNLQTLKLGQ-SGLPKNLRKLVNLRHLEFKMFGDT-AMPSDMGNLIHL 660
Query: 661 QTLSWFVAGFEEGCKIEELGNLKNLKGQLQLSNLEQVRSKEEALAAKLVNKKNLRELTFE 720
Q+LS F+ GFE+GCKIEELG LKNLKG+L L+NL +V++K+EA+AAKLV KKNLR L
Sbjct: 661 QSLSGFLVGFEKGCKIEELGPLKNLKGKLTLTNLWRVQNKDEAMAAKLVEKKNLRHLNL- 720
Query: 721 WSIDILRECSSYND--FEVLEGLQPPKNLSSLKITNFGGKFLPAATFVENLVFLCLYGCT 780
W + + D +VLEGLQP KNL SL+I F GK LP FVENLV + L
Sbjct: 721 WFFETDKRGEDDEDGIVQVLEGLQPHKNLQSLEILGFRGKVLPTGIFVENLVKIRLGHFE 780
Query: 781 KCERLPMLGQLANLQELSICFMDSVRSIGSEFYGIDSNRRG--YFPKLKKFDFCWMCNLE 840
+CE LPMLGQL NL+EL I +M+SVRSIG+EFYG+DS+ + FP+LKK M NLE
Sbjct: 781 RCEVLPMLGQLPNLKELEIMYMESVRSIGNEFYGVDSSHQNSVAFPQLKKLSIYEMMNLE 840
Query: 841 QWELEVANHESNHFGSLQTLKLDRCGKLTKLPNGLECCKSVHEVIISNCPNLTLNVEEMH 900
QW+ ESN FG L+ +++ RC L KLP+GLE C S+ + I C NL LNV+ +H
Sbjct: 841 QWDEATVVLESNLFGCLKEVRIRRCNPLAKLPSGLEGCHSLEYLSIRGCFNLMLNVQNLH 900
Query: 901 NLSVLLIDGLKFLPKGLALHPNLKTIMIKGCIEDYDYSPFLNLPS-LTKLYLNDGLGNA- 960
L L IDGLK LPKG+ LK + I GC+++Y++S ++L S L +L L+ G+
Sbjct: 901 KLYHLEIDGLKRLPKGMDGLTRLKELKIGGCMQNYEFSSVIHLASQLVELELSGRYGSVD 960
Query: 961 TQLPKQLQHLTALKILAIENFYGIEVLPEWLRKLTCLETLDLVRCKNLKRLPSRGAMRCL 1020
TQLP+QLQHLT L++L I F IE LPEW+ L L+TL C LK LPSR A+ L
Sbjct: 961 TQLPQQLQHLTNLQVLKITQFDCIEALPEWIGNLISLKTLKCSYCFKLKELPSREAILRL 1020
Query: 1021 TKLKDFKVIACPLLLLGGQADQEGAKYLHIPAYLCHVYQS 1035
TKL++ + CP LL+ G+ DQE AK H+P+ H +S
Sbjct: 1021 TKLENLDIFECPKLLV-GEGDQERAKLSHLPSKCVHKSES 1047
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
RGA2_SOLBU | 6.5e-116 | 32.78 | Disease resistance protein RGA2 OS=Solanum bulbocastanum GN=RGA2 PE=1 SV=1 | [more] |
RGA3_SOLBU | 5.1e-113 | 34.54 | Putative disease resistance protein RGA3 OS=Solanum bulbocastanum GN=RGA3 PE=2 S... | [more] |
RGA1_SOLBU | 8.4e-108 | 32.29 | Putative disease resistance protein RGA1 OS=Solanum bulbocastanum GN=RGA1 PE=2 S... | [more] |
RGA4_SOLBU | 4.6e-106 | 31.34 | Putative disease resistance protein RGA4 OS=Solanum bulbocastanum GN=RGA4 PE=2 S... | [more] |
R13L1_ARATH | 2.4e-94 | 29.34 | Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana GN=RPPL... | [more] |
Match Name | E-value | Identity | Description | |
AT3G14470.1 | 1.3e-95 | 29.34 | NB-ARC domain-containing disease resistance protein | [more] |
AT3G14460.1 | 7.4e-86 | 29.44 | LRR and NB-ARC domains-containing disease resistance protein | [more] |
AT1G53350.1 | 2.8e-61 | 25.59 | Disease resistance protein (CC-NBS-LRR class) family | [more] |
AT3G50950.2 | 9.1e-60 | 28.15 | HOPZ-ACTIVATED RESISTANCE 1 | [more] |
AT5G43470.1 | 1.8e-52 | 25.92 | Disease resistance protein (CC-NBS-LRR class) family | [more] |