Csa4G006250 (gene) Cucumber (Chinese Long) v2 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Csa4G006250
Type	gene
Organism	Cucumis. sativus (Cucumber (Chinese Long) v2)
Description	Thymidylate kinase; contains IPR018094 (Thymidylate kinase), IPR027417 (P-loop containing nucleoside triphosphate hydrolase)
Location	Chr4 : 999301 .. 1006519 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTTGCTTCCACATTCTTCAAGCCATTGTAAGATTAATGTCTTGTTTTACTTGTAATATATGGTTTTTTTTTTTTTTTTTTTTTTGAGATAATTATGATTATATACTGGGAAAGATTTTATTTGTCCTTAAAAGTGTGAAGTCTATCATTTTTACCCTTAAAAGTGCAAGTTTACCATTTTCCCCCATAAACCTTCAATTTCTATTTCATCATATAGGACACTAGACTTTGATAAATGTTGTAATTTTTAACATCTATTCATTTTTAGCTAATTCAATTACTAATTACAACATAATGACGATGCAAAAATCTCCCTTTACCACCAAGTCCAGTAATATTAAAATTTGATTTTAGTTTAGTTGTGTGCAAACTAGCCATTGCATAACATTATCAAAACTTTAAAATTTTAACAGAAAATATTGCTCTCATGTATGCTTTTTATAAAATCCTATTATAGATGATATATGAGTTTTGTAATTCATGGTACTTATGTATATGAGGATGCTTTTATTGTTATTATTATTACTACTACTATTATTATTTAATTATTTTTTGAACCTTTGGTAAGGAGTGCATACCAATATTCCTGAGCTATGCTCACTTTGGCTTATTAGAATATTTCTTTTAATGATATTTGGAATGTAAACGTATCATAGAGGAAAAGTTATAAGCCTTTTTTAAGTTTAAGGTTAAGATGACAACATTCAACTAAGTTTGAGGTGCAATTTGATGGTTTAGAGTAAAAATTAATACAATATGACAAGTGTGCTGGTAAACATAGAATTTTTTTTCCCTATATATCTGTTTTTGGGGGCTATGATGTGTTGGTGAGAAATACATTTGAATCTTTTCTTGAAGGTATCTTTTTCTAATAAAAATCTAATGTGCATAAAATAGTTTCTGCTTTAATCATGAATATTTCCAAATTTCTTTTGAAAATTATTGATAAAGAAGACCTCTTTAATGTTTGTTTTTCCTATTTAGGAATTTTGGAGTAGCAACAACTAGAATATCCTCGAGTTTTCGACCAAAAGTTGTGTGTAAAAATTTTATTCGGAAGATCCAAATGGGAACTAATCAGAACTCCATCTCAAGAGATTCTAGCAGCAACTCTAGAGGAGCCCTGGTGGTTCTAGAAGGGTTGGATCGATGTGGGAAGACTACACAGGCCAGTAGATTAGCCCAGTACTTGGAAGGACTTGGCCATTCTGTTGAACTATGGCGGTTTCCTGACAGAACCACTAGCGTTGGACAGATGATATCTTCCTATCTTTCTAATGAATCACAGCTGGATGACCACACAATTCATCTTCTTTTCAGTGCTAACCGATGGGAGAAGAGGTTTTCATTTTTTCTTTATACAACTTGCATACTAGTCTCTTAGGTTGAGAAATTTTCAACTCCTAACAAATGATGGAGCTTTCTATATTGATACTAAGTAGCAAGCATTAAAATGAAAAATATTGATACTTGGTTTTAGAAGTTCCAAGCAACAGAAGTTGCAAATTATGACCCATAACCATTTTGTTGCTTGTAATATAAGGGTATAATATATTTCTACCCCAATAGTATGTGCTTTGTGTTTAATTACTATAATGCCCATGCAGATCCTTAATGGAAACTAAGTTGAAAGGTGGAACCACAATTATTCTTGATCGGTATTCTTACTCAGGGGTGGCCTTTTCATGTGCCAAAGGACTTAACTTTGAGTGGTGTAAGGTAAAAATACAAATAAACAATTTGGATGAATCCTTTGTTTATTTATATGATATAAATGTTTCGTTTCTATTTGTATTTTTAATGTGGAATGCTAAAATTTTGAAGTTATGTAATGGTCCATTTCACCCATTTTTCTTTTTGCAAAGGTATTGTAATAAATGCTGGGGTGGGGATTTGAACCCAGGACCTCTTAACATACAGGTGCCAGTTGAGCTAAGCTCATGTTGGCCCATTTCACCCATTTTGTAATTTAATTTGTACAACTCATTTCTTCTTTGTGGTGTCTAAGTCAAAAACCTTTTTCGTAATGATGGCTTAGCTTTGAACCTCCATGTTTTAACGCTTTCCTTCACTATATGTTGTGAGGGACCCTTATCGTTCCCCTATGCCCAAGTGAATTTTGTTATAGTATTATATTCCTGAAGTATGAAGAAACCATTTAGATGCTCTCTTCTCTCTCCACCCAGCTTGTCACCTCTAGCCTTCAAAAACTCTGATTTTGTCTTGTCTCACTGATGTTTGTGGCCTCCAGTCCCAAAGAGCTTTGTCAGATAATTATAGTTAAGTTGGAATGTTCTATTTGGGTGATCCAACGAACATTATTCATTGGAACATTAGAGGTTTGGAAGATAGCTCTAAAAGAGTTAGTTGAAGTGTTTTCTGCATATTTGTTCTGAGTGGATTCGTGTTAAAGTAACCTAAAAATCCTCTTTCAATAGAAAATTGATCAAATTATTATGGAGTTCAAGAGATTTAAGTTGGATTCGTGTCAGGTGGTTTGTTAATTTGTGAGATGAAAGCAAAGTGAAGGTTGTTGAGGTTTATTTGGAGGATATTCTCTCTTTGTATCTTTTCTACTAAATAAAGTTTGTTGGATTTCAAACATTTACGAACCAAATGATTATGAATGAAAGTGTCTTTGGAGTGAATTACGTTCTTTCATTCTTATTGCAAGGAACCATAGTGTGCAGGCGGAGATTTCAAAATAATCAGATGGGTTCATTAGTGATTTCCTATCTGTAGACAAACAAGTTTATGAGGCAGTTTAATAAAATTATTGAAGAACTAGGCTTTGTGGAAATCTCTTTGTCCAGTGGAAAATTCACATGGTTAAGAGAAGGTAATGGTGTACAATCTTTGTCAGATGGATTTGTTTGTTTCTGTTGAGTGGGATATTGTGGTTGAAAATTGAAGGATGTCAAAGCAGGTAATTTTTGCTGACCATTCTCCTCTTCTTTTCGAAGTAGGTAGTTTTGTATGGGGGGCTTCACCTTTCTAAAGTTCATGGATTATGGGGGGAATAGATCCTATGGCGGGGCAGGTTTTGTTATTTCTTCTGAACTCTGAAATCTGAAAACATCTACCAAGGGGAAGAACTTATTTTCTGAAATTACATATTTTGCTGCCAAATAAGAATTGGGGCTACTGACTGGTAAAGAATGGTGTCTTAGGTATTGAAAGGCAAGTTATTGGATCTCTACATGATTGAAGAAAGAAGTTTGATTCAGAAATGTCTGCAACTCTGCATGGAGAGGATGTGATTATGAATACAGCTTCGTTCATTGTTTTTTGGCAGCTAAAAAAGGCTTCTTATTTCTGAATTATGCTCCGTTGGTGGAAGGTGGTTTCAAGTCCAGCAAATGAGGCTCTAATTGTTGCTTTCTCTCTCGATGAAATTTTGCATGATATGAAAATCTTGGGCAAGAAAAGTCTCTTGAGTCTTGGGCTGCAGTAATTATGGATATTCACAGGCATTTTCAATTTGAATGACACACAACTGGAAGGTATGGTTCAAGCCTCAGAAGCCCATGGACCAGTATTTCTAAGAATTGGAAATGCTACGATTTCTTTGCCTCCTTTAAATTGGCAAATGGAAATCCTATTTTTGTCTAAAATTCCATTTTTCCTCACTTATTTGCTATCTCTACATCTCCGAACGGCTCAGTTTTGGACCTATGGGACAATCAACATCTCTCATGGTTTTTAGTTTTTTGAAGCCTTTTGAAAGAAGATGAAATTATTGCTTTGCAATCTTTGTTGGGTTGGGTATTCTGATAACAAAAATATTGGCTCTATTGAAGACTCAAGGCAGTGGCCTTTTACATTCATCTAGTTTGTGCTTGATGAAATTCTTATCAAGATAGTGTTTTTTTTTTCTTTGCAAAAGGATTTATTTGAGACCATTTGGAAGTGTTATAGTGCCAAACATTTAAATGTGCGACTGTGGATCAGTGTAAATGGTAGTTATATCTTAAGTCATTGGCATGCCCTCTTTGTCATGCAGATGGAGATTATCTTTCTCATATTTTCTTAGCTGCTCTTATTGTCAAAGTGTTGGTTCAAGCTCCTCTCTGATTCCAAAATCGAGTTGGTATTCTCATGTGTCTTAAGTGAAAAAGTTTTCCAGCTGATCGTTGGCTAAAAAACAATTTTATTATGGATTAATGTGGTATCCTTGGGAAGAAGAAATAACAAGATTTTCGAAGATAAGTTCGGTTCTTTTGATTGGACCTTTGTTCAACACATGACCTCTTGGTGGTGTACAAACTACACCAAGTTATTTTGTAACTACACTTTTGATTAACTTTAACAATTGGAAGGCTCTCTCCTTCGTTGGCTCCTTGGGATGAGTCCTCTCAATCTCTTGCCCTTAATATGCCATTCTGTTTCTTATAAAAAAATTATGGTTTTATGTGGTTAAAGCCAAATGTGGAAAGGAATCAACTAATCTTTGAAGATAAATATCTTCATGTGGTTTGATTGGTTTGAATATGCAAGATTAAAAGTCTCTACGTGGTGTTCCTTTCCAAATTCTTTACTCATTACTCTTCCTAATTTAGAATCTGTCCCAACATTCACCCACAACAATGCCATCCTTGCTTTTCTCAAAGCTTTTAAATATGAAGAGACTATCCCCACAAATCAAGACGGTTCTTTTCAGCATGTTTTTTCCTCACTCACATAGGAAATGTAATTTTTCAAGAGGCCACCGAACATAGAATTGCTTCAACCTGAGCTCGCTTAACTTCAAAGTCTCTATGATTGAACCACCGAAAAGGAAGGTACAAGTTTAGGTAGTAACTTTCACTTCTTTTAAGTGCTTCTTAACCATAGTTCATCTTTTCAGGATCTCTCGCATTTGGAAGTAATTTTGTTCATTCATGTATAACACTGGTGTTACAAAAAGCCACTACTTGGGGTTCCCTTTCCAGATTGTTTACTAATTACTTTTCCTATGATATATGTCTTAAGTGAGAGGCTTTTCTTTCTCCTTCATAACTCATGCCTTTTTATTTATTTTCTTTTTTCACTTCCTAGTGGGGTATTGGGGTTATATTTTGAGCATTAGTCTCTTTTCATTACTCAATGAAAAGTTTTGATTTCCGGTTTAGAAAAGCAATATATTCCTGTTGGTTCATGTCCAACAAAAAGAAAAAAGGAAATGCTGATGTCATGTTTTTCCTTTGCAGGCGCCAGAGGTTGGCTTGCTAGCTCCAGATCTAGTACTTTACCTAAACATATCTCCAAAGGTCGGTTAAATCATTACATCATTTCATTTTGGAACAATTTTAAATGCATTTATTAGGATGGTTCATCATGTTTTCCAACTTGTAAATGTACTGAAGCTACATATTGACATTTTTGGTTTGGGACCCTTAAGTCATAGATGATATAACGACTTTCAACCATGTCTGCACATATAGATATAGGAGCAATTGTAAACTTTGTATTGGATTTTTGTATGGAACTCAATGAATATCTGGTTTGTAGAATACAGTAAGTCCAGTAGACCAGAGGTTATTAGAGACTTATTTGAAAGTTTTATTAGGTCATTGATGACGATGGATTTAAAAACAATAAATTAATATCCCAGAGAATAATGTTGTTTTGTACCATATATGATATCAAAATATTATACTATCTGCGTAACTTTATCTAGGAGTATTGCCATTTTTTATGCAAATGATGAAACCTGACTTTGATTTACAACCCTCACCAATCAGACAAATTCTCAAGTTAAAAAACGTTCACTCTGTACAGTGATTGCTTGGTGAATAAAGAGTTATTTTGAATGATCAGATGGCTGCAGAAAGAAAAGGATACGGAAGTGAGAGATACGAGCAGATTGAGTTCCAGAACCAAGTTGTTGAGGCCTATCGAGATTTGATGATTGGTTCTAATTGGCAGGTAACTTGTTTTACATTTGTGTCTTGGAAACATTGATACGCCAAGACTGTGAGATTGGTGTGTGGTATGTGTTTAGGATGCCTAGACGTGGACACTCCTTGCCCCATGGGCATGTGCCCAACACCTTAATTATTATCTGGTTATTTAATTATTTTTTTAAAACTTGGTTGACATGGCTTGAACACTTTTTGGGGAAGTTAGATAAACTAGAAAAGAAGCCTTAGCTGATCCACATACAAAAGCTTCCAGTAGCCAATTTGAGACGGACAAATTCTTGACGACGAAGTAGTTTTAAATTTCTCAACATTGGTTCATCAATGGGGATGAACGTTCAACATTTTCTAACATGGATGGGCTTCAAATATAAATAGGAAGGGCATGATACAGTGAAGGCACCTCCCAGAATGCTGGGGGAGAGATTGAATCGACATGGATAAACCACTACAATTTTTTTAGAGCAGATGGTAGTCCCTGATCCAAAGGCCTAGATTGGCACTATATAATTAGTAGTTGGAGTTGATGGCTTGAAAGACTTTTCTTGGATGTATAAATAGTGGGAGTGGTAAAGATAAGATGATAGGGACCAAGATGGTTTCTTAGCAGAACTTTTTTTAAAAGCTTAGAATATCGTCAGTATCAATTTGATTGAGATGTTTCAAGAGTTGTTTGAAAATGGTCTCATAGATGGAAGCATAAATTAAAATTATCACAAGAGGGTGGCTGAAATGTGGAAGTGTAAGACGACTACTTATAGGAATAGTAGAATTTTTAGTAGAAAGTTAATATGGTTATTTGTTGGGGCATATTAATACTTAGTAATTACCGGGTGTGGCCAAAAATAGGGAAGGGGTAACAAGACTCCCAAAAATAAGGAGGAAAAGAAAGGGGCATATTAATTTTCTACTAGTAATTCTACTATCCCTATAACTAGGTGTCTTAAAGAAAGACTTTTAAACCTATGAAGTTAGTGGTCATATGACTATTTCAACTATGAGGTCCTGTGGAGCTAACGGATGAGAATTTCCGATATATATAACCAAGATAGAAAGTTCCTTTGGTGACTTCGTCTAATCTCCCACATTCAACGAATTTATTGATGATGAAAGATTGCTTTGGGCTTTATATTCGACTTATTTAAGCTGGTTTGTTTGCCGACTGTATTTTTGAACTTTCCTTCGTTGATTTAATTGCAGATTGTTGATGCTTGCCAACCCATGGAAGTTATTGAGAAAGAGCTACAGGAGATTGTCCTGGACTGTGTCAAGAAATGCCAAAATGGGGCACCTCTTTCCGATCTTTGGTCAAATTGA

mRNA sequence

ATGCTTGCTTCCACATTCTTCAAGCCATTGAATTTTGGAGTAGCAACAACTAGAATATCCTCGAGTTTTCGACCAAAAGTTGTGTGTAAAAATTTTATTCGGAAGATCCAAATGGGAACTAATCAGAACTCCATCTCAAGAGATTCTAGCAGCAACTCTAGAGGAGCCCTGGTGGTTCTAGAAGGGTTGGATCGATGTGGGAAGACTACACAGGCCAGTAGATTAGCCCAGTACTTGGAAGGACTTGGCCATTCTGTTGAACTATGGCGGTTTCCTGACAGAACCACTAGCGTTGGACAGATGATATCTTCCTATCTTTCTAATGAATCACAGCTGGATGACCACACAATTCATCTTCTTTTCAGTGCTAACCGATGGGAGAAGAGATCCTTAATGGAAACTAAGTTGAAAGGTGGAACCACAATTATTCTTGATCGGTATTCTTACTCAGGGGTGGCCTTTTCATGTGCCAAAGGACTTAACTTTGAGTGGTGTAAGGCGCCAGAGGTTGGCTTGCTAGCTCCAGATCTAGTACTTTACCTAAACATATCTCCAAAGATGGCTGCAGAAAGAAAAGGATACGGAAGTGAGAGATACGAGCAGATTGAGTTCCAGAACCAAGTTGTTGAGGCCTATCGAGATTTGATGATTGGTTCTAATTGGCAGATTGTTGATGCTTGCCAACCCATGGAAGTTATTGAGAAAGAGCTACAGGAGATTGTCCTGGACTGTGTCAAGAAATGCCAAAATGGGGCACCTCTTTCCGATCTTTGGTCAAATTGA

Coding sequence (CDS)

ATGCTTGCTTCCACATTCTTCAAGCCATTGAATTTTGGAGTAGCAACAACTAGAATATCCTCGAGTTTTCGACCAAAAGTTGTGTGTAAAAATTTTATTCGGAAGATCCAAATGGGAACTAATCAGAACTCCATCTCAAGAGATTCTAGCAGCAACTCTAGAGGAGCCCTGGTGGTTCTAGAAGGGTTGGATCGATGTGGGAAGACTACACAGGCCAGTAGATTAGCCCAGTACTTGGAAGGACTTGGCCATTCTGTTGAACTATGGCGGTTTCCTGACAGAACCACTAGCGTTGGACAGATGATATCTTCCTATCTTTCTAATGAATCACAGCTGGATGACCACACAATTCATCTTCTTTTCAGTGCTAACCGATGGGAGAAGAGATCCTTAATGGAAACTAAGTTGAAAGGTGGAACCACAATTATTCTTGATCGGTATTCTTACTCAGGGGTGGCCTTTTCATGTGCCAAAGGACTTAACTTTGAGTGGTGTAAGGCGCCAGAGGTTGGCTTGCTAGCTCCAGATCTAGTACTTTACCTAAACATATCTCCAAAGATGGCTGCAGAAAGAAAAGGATACGGAAGTGAGAGATACGAGCAGATTGAGTTCCAGAACCAAGTTGTTGAGGCCTATCGAGATTTGATGATTGGTTCTAATTGGCAGATTGTTGATGCTTGCCAACCCATGGAAGTTATTGAGAAAGAGCTACAGGAGATTGTCCTGGACTGTGTCAAGAAATGCCAAAATGGGGCACCTCTTTCCGATCTTTGGTCAAATTGA

Protein sequence

MLASTFFKPLNFGVATTRISSSFRPKVVCKNFIRKIQMGTNQNSISRDSSSNSRGALVVLEGLDRCGKTTQASRLAQYLEGLGHSVELWRFPDRTTSVGQMISSYLSNESQLDDHTIHLLFSANRWEKRSLMETKLKGGTTIILDRYSYSGVAFSCAKGLNFEWCKAPEVGLLAPDLVLYLNISPKMAAERKGYGSERYEQIEFQNQVVEAYRDLMIGSNWQIVDACQPMEVIEKELQEIVLDCVKKCQNGAPLSDLWSN*

Homology

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BLAST of Csa4G006250 vs. Swiss-Prot
Match: ZEUS1_ARATH (Thymidylate kinase OS=Arabidopsis thaliana GN=ZEU1 PE=2 SV=1)

HSP 1 Score: 306.6 bits (784), Expect = 2.6e-82
Identity = 148/226 (65.49%), Postives = 181/226 (80.09%), Query Frame = 1


Query: 35  KIQMGTNQNSISRDSSSNSRGALVVLEGLDRCGKTTQASRLAQYLEGLGHSVELWRFPDR 94
           +++     + +  +SS   RGAL+VLEGLDR GK+TQ ++L  +L GLGH  ELWRFPDR
Sbjct: 39  RMESSNFSSGVRTESSVKPRGALIVLEGLDRSGKSTQCAKLLSFLNGLGHPTELWRFPDR 98

Query: 95  TTSVGQMISSYLSNESQLDDHTIHLLFSANRWEKRSLMETKLKGGTTIILDRYSYSGVAF 154
            TSVGQMIS+YLSN+SQLDDHTIHLLFSANRWEKRSLME KLK GTT+I+DRYSYSGVAF
Sbjct: 99  ETSVGQMISAYLSNKSQLDDHTIHLLFSANRWEKRSLMEEKLKTGTTLIVDRYSYSGVAF 158

Query: 155 SCAKGLNFEWCKAPEVGLLAPDLVLYLNISPKMAAERKGYGSERYEQIEFQNQVVEAYRD 214
           S AKGL+ EWCKAPE+GLLAPD VLYL+ISP+ AAER GYG ERYE++EFQ +V + Y+ 
Sbjct: 159 SSAKGLDIEWCKAPEIGLLAPDSVLYLDISPERAAERGGYGDERYERVEFQKKVADFYQT 218

Query: 215 LMIGSNWQIVDACQPMEVIEKELQEIVLDCVKKCQNGAPLSDLWSN 261
           L   S+W+I++A   ME +EK++Q++VLD VK+C  G PLS LWS+
Sbjct: 219 LG-DSSWKIINASDAMEEVEKKIQQVVLDQVKECTEGKPLSLLWSS 263

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BLAST of Csa4G006250 vs. Swiss-Prot
Match: KTHY_MOUSE (Thymidylate kinase OS=Mus musculus GN=Dtymk PE=1 SV=2)

HSP 1 Score: 197.6 bits (501), Expect = 1.7e-49
Identity = 98/211 (46.45%), Postives = 142/211 (67.30%), Query Frame = 1


Query: 51  SNSRGALVVLEGLDRCGKTTQASRLAQYLEGLGHSVELWRFPDRTTSVGQMISSYLSNES 110
           ++ RGAL+VLEG+DR GKTTQ  +L   L   GH  EL RFP+R+T +G++++SYL  ++
Sbjct: 2   ASRRGALIVLEGVDRAGKTTQGLKLVTALCASGHRAELLRFPERSTEIGKLLNSYLEKKT 61

Query: 111 QLDDHTIHLLFSANRWEKRSLMETKLKGGTTIILDRYSYSGVAFSCAK-GLNFEWCKAPE 170
           +L+DH++HLLFSANRWE+  L++ KL  G T++LDRY++SGVAF+ AK   + +WCK P+
Sbjct: 62  ELEDHSVHLLFSANRWEQVPLIKAKLNQGVTLVLDRYAFSGVAFTGAKENFSLDWCKQPD 121

Query: 171 VGLLAPDLVLYLNISPKMAAERKGYGSERYEQIEFQNQVVEAYRDLM--IGSNWQIVDAC 230
           VGL  PDL+L+L +    AA R  +G ERYE   FQ QV+  ++ LM     NW++VDA 
Sbjct: 122 VGLPKPDLILFLQLQLLDAAARGEFGLERYETGTFQKQVLLCFQQLMEEKNLNWKVVDAS 181

Query: 231 QPMEVIEKELQEIVLDCVKKCQNGAPLSDLW 259
           + +E + KE++    D ++      PL +LW
Sbjct: 182 KSIEEVHKEIRAHSEDAIRNAAQ-RPLGELW 211

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BLAST of Csa4G006250 vs. Swiss-Prot
Match: KTHY_HUMAN (Thymidylate kinase OS=Homo sapiens GN=DTYMK PE=1 SV=4)

HSP 1 Score: 196.8 bits (499), Expect = 2.9e-49
Identity = 94/208 (45.19%), Postives = 142/208 (68.27%), Query Frame = 1


Query: 54  RGALVVLEGLDRCGKTTQASRLAQYLEGLGHSVELWRFPDRTTSVGQMISSYLSNESQLD 113
           RGAL+VLEG+DR GK+TQ+ +L + L   GH  EL RFP+R+T +G+++SSYL  +S ++
Sbjct: 5   RGALIVLEGVDRAGKSTQSRKLVEALCAAGHRAELLRFPERSTEIGKLLSSYLQKKSDVE 64

Query: 114 DHTIHLLFSANRWEKRSLMETKLKGGTTIILDRYSYSGVAFSCAK-GLNFEWCKAPEVGL 173
           DH++HLLFSANRWE+  L++ KL  G T+++DRY++SGVAF+ AK   + +WCK P+VGL
Sbjct: 65  DHSVHLLFSANRWEQVPLIKEKLSQGVTLVVDRYAFSGVAFTGAKENFSLDWCKQPDVGL 124

Query: 174 LAPDLVLYLNISPKMAAERKGYGSERYEQIEFQNQVVEAYRDLMIGS--NWQIVDACQPM 233
             PDLVL+L +    AA+R  +G ERYE   FQ + +  +  LM  +  NW++VDA + +
Sbjct: 125 PKPDLVLFLQLQLADAAKRGAFGHERYENGAFQERALRCFHQLMKDTTLNWKMVDASKSI 184

Query: 234 EVIEKELQEIVLDCVKKCQNGAPLSDLW 259
           E + ++++ +  D ++      PL +LW
Sbjct: 185 EAVHEDIRVLSEDAIRTATE-KPLGELW 211

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BLAST of Csa4G006250 vs. Swiss-Prot
Match: KTHY_DICDI (Thymidylate kinase OS=Dictyostelium discoideum GN=dtymk PE=3 SV=1)

HSP 1 Score: 186.0 bits (471), Expect = 5.1e-46
Identity = 88/213 (41.31%), Postives = 133/213 (62.44%), Query Frame = 1


Query: 38  MGTNQNSISRDSSSNSRGALVVLEGLDRCGKTTQASRLAQYLEGLGH-SVELWRFPDRTT 97
           + T +     + +   RG  ++ EG+DR GK+TQ   L  ++  +     +  RFPDRTT
Sbjct: 2   INTKKEEGEEEKTMIKRGLFILFEGVDRVGKSTQVQSLTNHISNVQKLPTKSLRFPDRTT 61

Query: 98  SVGQMISSYLSNESQLDDHTIHLLFSANRWEKRSLMETKLKGGTTIILDRYSYSGVAFSC 157
            +GQ+I+ YL N + +DD  +HLLFS+NRWE R  +   L  GT I++DRYSYSGVA+S 
Sbjct: 62  PIGQIINQYLQNATNMDDRALHLLFSSNRWEARDSILELLNNGTNIVVDRYSYSGVAYSA 121

Query: 158 AKGLNFEWCKAPEVGLLAPDLVLYLNISPKMAAERKGYGSERYEQIEFQNQVVEAYRDLM 217
           AKG++F+WC A E GL  PDL+ YL++S + A +R  YG ERYE++EFQ ++ + Y + +
Sbjct: 122 AKGIDFDWCYACEKGLPKPDLIFYLSMSSEDATKRGEYGGERYEKLEFQKKIKQIYEEKL 181

Query: 218 IGSNWQIVDACQPMEVIEKELQEIVLDCVKKCQ 250
           +   W+I++A + ++ I  E+  I     KK Q
Sbjct: 182 VDDQWKIINANRSIDEISNEISSIFDSEFKKIQ 214

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BLAST of Csa4G006250 vs. Swiss-Prot
Match: KTHY_SCHPO (Thymidylate kinase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=tmp1 PE=2 SV=2)

HSP 1 Score: 185.3 bits (469), Expect = 8.7e-46
Identity = 91/197 (46.19%), Postives = 128/197 (64.97%), Query Frame = 1


Query: 50  SSNSRGALVVLEGLDRCGKTTQASRLAQYLEGLGHSVELWRFPDRTTSVGQMISSYLSNE 109
           S  +RG L+V+EGLDR GK+TQ   L   L       EL++FPDRTT++G+ I  YL   
Sbjct: 2   SKQNRGRLIVIEGLDRSGKSTQCQLLVDKLISQHEKAELFKFPDRTTAIGKKIDDYLKES 61

Query: 110 SQLDDHTIHLLFSANRWEKRSLMETKLKGGTTIILDRYSYSGVAFSCAKGLNFEWCKAPE 169
            QL+D  IHLLFSANRWE    +  ++  G T ILDRY++SG+AFS AKGL++EWCK+P+
Sbjct: 62  VQLNDQVIHLLFSANRWETIQYIYEQINKGVTCILDRYAFSGIAFSAAKGLDWEWCKSPD 121

Query: 170 VGLLAPDLVLYLNISPKMAAERKGYGSERYEQIEFQNQVVEAYRDLM-----IGSNWQIV 229
            GL  PDLV++LN+ P++AA R  YG ERYE+IE Q +V++ ++ L       G  +  +
Sbjct: 122 RGLPRPDLVIFLNVDPRIAATRGQYGEERYEKIEMQEKVLKNFQRLQKEFREEGLEFITL 181

Query: 230 DACQPMEVIEKELQEIV 242
           DA   +E +  ++ ++V
Sbjct: 182 DASSSLEDVHSQIVDLV 198

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BLAST of Csa4G006250 vs. TrEMBL
Match: A0A0A0KTN6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G006250 PE=3 SV=1)

HSP 1 Score: 525.0 bits (1351), Expect = 5.2e-146
Identity = 260/260 (100.00%), Postives = 260/260 (100.00%), Query Frame = 1


Query: 1   MLASTFFKPLNFGVATTRISSSFRPKVVCKNFIRKIQMGTNQNSISRDSSSNSRGALVVL 60
           MLASTFFKPLNFGVATTRISSSFRPKVVCKNFIRKIQMGTNQNSISRDSSSNSRGALVVL
Sbjct: 1   MLASTFFKPLNFGVATTRISSSFRPKVVCKNFIRKIQMGTNQNSISRDSSSNSRGALVVL 60

Query: 61  EGLDRCGKTTQASRLAQYLEGLGHSVELWRFPDRTTSVGQMISSYLSNESQLDDHTIHLL 120
           EGLDRCGKTTQASRLAQYLEGLGHSVELWRFPDRTTSVGQMISSYLSNESQLDDHTIHLL
Sbjct: 61  EGLDRCGKTTQASRLAQYLEGLGHSVELWRFPDRTTSVGQMISSYLSNESQLDDHTIHLL 120

Query: 121 FSANRWEKRSLMETKLKGGTTIILDRYSYSGVAFSCAKGLNFEWCKAPEVGLLAPDLVLY 180
           FSANRWEKRSLMETKLKGGTTIILDRYSYSGVAFSCAKGLNFEWCKAPEVGLLAPDLVLY
Sbjct: 121 FSANRWEKRSLMETKLKGGTTIILDRYSYSGVAFSCAKGLNFEWCKAPEVGLLAPDLVLY 180

Query: 181 LNISPKMAAERKGYGSERYEQIEFQNQVVEAYRDLMIGSNWQIVDACQPMEVIEKELQEI 240
           LNISPKMAAERKGYGSERYEQIEFQNQVVEAYRDLMIGSNWQIVDACQPMEVIEKELQEI
Sbjct: 181 LNISPKMAAERKGYGSERYEQIEFQNQVVEAYRDLMIGSNWQIVDACQPMEVIEKELQEI 240

Query: 241 VLDCVKKCQNGAPLSDLWSN 261
           VLDCVKKCQNGAPLSDLWSN
Sbjct: 241 VLDCVKKCQNGAPLSDLWSN 260

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BLAST of Csa4G006250 vs. TrEMBL
Match: A0A0D2SFC9_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_007G185700 PE=3 SV=1)

HSP 1 Score: 335.1 bits (858), Expect = 7.6e-89
Identity = 165/253 (65.22%), Postives = 199/253 (78.66%), Query Frame = 1


Query: 7   FKPLNFGVATTRISSSFRPKVVCKNFIRKIQMGTNQNSISRDSSSNSRGALVVLEGLDRC 66
           F  LNFGV   R S +    + CK  +R I+M    N     +S+ SRGALVV EGLDR 
Sbjct: 7   FPSLNFGVLAVRRSLNLGLNISCKRSVRSIRMENGNNCNLMGASNESRGALVVFEGLDRS 66

Query: 67  GKTTQASRLAQYLEGLGHSVELWRFPDRTTSVGQMISSYLSNESQLDDHTIHLLFSANRW 126
           GKT+Q  +L  YLEGLGHSVELWRFPDRTTSVGQMIS+YL+N+SQLDDHTIHLLFSANRW
Sbjct: 67  GKTSQCGKLLSYLEGLGHSVELWRFPDRTTSVGQMISAYLTNKSQLDDHTIHLLFSANRW 126

Query: 127 EKRSLMETKLKGGTTIILDRYSYSGVAFSCAKGLNFEWCKAPEVGLLAPDLVLYLNISPK 186
           EKRS+ME KLK GTT+I+DRYSYSGVAFS AKGL+FEWCKAPE GL+APDLV+YL+I P+
Sbjct: 127 EKRSMMEAKLKAGTTLIVDRYSYSGVAFSSAKGLDFEWCKAPENGLIAPDLVVYLDIPPE 186

Query: 187 MAAERKGYGSERYEQIEFQNQVVEAYRDLMIGSNWQIVDACQPMEVIEKELQEIVLDCVK 246
            AAER GYG ERYEQ+EFQ +V + Y+ L+  S+W+++DA Q +E +EK+++EIVL+ V 
Sbjct: 187 KAAERGGYGGERYEQLEFQKKVAQHYK-LLEDSSWKMIDASQCIEDVEKQVEEIVLEHVV 246

Query: 247 KCQNGAPLSDLWS 260
            CQ G PLS LWS
Sbjct: 247 TCQKGKPLSSLWS 258

[Close]

BLAST of Csa4G006250 vs. TrEMBL
Match: A0A061EG90_THECC (P-loop containing nucleoside triphosphate hydrolases superfamily protein OS=Theobroma cacao GN=TCM_019290 PE=3 SV=1)

HSP 1 Score: 335.1 bits (858), Expect = 7.6e-89
Identity = 169/257 (65.76%), Postives = 201/257 (78.21%), Query Frame = 1


Query: 5   TFFKPLNFGVATTRISSSFRPKVVCKNFIRKIQMGTNQNSISRDSSSN-SRGALVVLEGL 64
           T  K LN G    R S +       K  +R I++  N N+ S   + N SRGALVV EGL
Sbjct: 9   TVSKALNLGAFAVRRSLNLGSNFSYKCSVRSIRIMENANTSSLMGADNESRGALVVFEGL 68

Query: 65  DRCGKTTQASRLAQYLEGLGHSVELWRFPDRTTSVGQMISSYLSNESQLDDHTIHLLFSA 124
           DR GKT+Q  RL  YLEGLGHSVELWRFPDRTTSVGQMIS+YL+N SQLDD TIHLLFSA
Sbjct: 69  DRSGKTSQCGRLVSYLEGLGHSVELWRFPDRTTSVGQMISAYLTNNSQLDDRTIHLLFSA 128

Query: 125 NRWEKRSLMETKLKGGTTIILDRYSYSGVAFSCAKGLNFEWCKAPEVGLLAPDLVLYLNI 184
           NRWEKRSLMETKLK GTT+I+DRYSYSGVAFS AKGL+FEWCKAPE+GL+APDLV+YL+I
Sbjct: 129 NRWEKRSLMETKLKAGTTLIVDRYSYSGVAFSSAKGLDFEWCKAPEIGLIAPDLVVYLDI 188

Query: 185 SPKMAAERKGYGSERYEQIEFQNQVVEAYRDLMIGSNWQIVDACQPMEVIEKELQEIVLD 244
            P+ AAER GYG ERYEQ+EFQ +V + Y+ L+  S+W+IVDACQP+E +EK+++EIVL+
Sbjct: 189 VPEKAAERGGYGGERYEQLEFQRKVAQRYK-LLEDSSWKIVDACQPLEDVEKQVKEIVLE 248

Query: 245 CVKKCQNGAPLSDLWSN 261
            V  CQ G P+S LWS+
Sbjct: 249 HVMTCQRGKPVSSLWSS 264

[Close]

BLAST of Csa4G006250 vs. TrEMBL
Match: A0A0B0N9J7_GOSAR (Thymidylate kinase OS=Gossypium arboreum GN=F383_05248 PE=3 SV=1)

HSP 1 Score: 334.3 bits (856), Expect = 1.3e-88
Identity = 164/253 (64.82%), Postives = 199/253 (78.66%), Query Frame = 1


Query: 7   FKPLNFGVATTRISSSFRPKVVCKNFIRKIQMGTNQNSISRDSSSNSRGALVVLEGLDRC 66
           F  LNFGV   R S +    + CK  +R I+M    N     +S+ SRGALVV EGLDR 
Sbjct: 7   FPSLNFGVLAVRRSLNLGLNISCKRSVRSIRMENGNNCNLMGASNESRGALVVFEGLDRS 66

Query: 67  GKTTQASRLAQYLEGLGHSVELWRFPDRTTSVGQMISSYLSNESQLDDHTIHLLFSANRW 126
           GKT+Q  +L  YLEG+GHSVELWRFPDRTTSVGQMIS+YL+N+SQLDDHTIHLLFSANRW
Sbjct: 67  GKTSQCGKLLSYLEGMGHSVELWRFPDRTTSVGQMISAYLTNKSQLDDHTIHLLFSANRW 126

Query: 127 EKRSLMETKLKGGTTIILDRYSYSGVAFSCAKGLNFEWCKAPEVGLLAPDLVLYLNISPK 186
           EKRS+ME KLK GTT+I+DRYSYSGVAFS AKGL+FEWCKAPE GL+APDLV+YL+I P+
Sbjct: 127 EKRSMMEAKLKAGTTLIVDRYSYSGVAFSSAKGLDFEWCKAPENGLIAPDLVVYLDIPPE 186

Query: 187 MAAERKGYGSERYEQIEFQNQVVEAYRDLMIGSNWQIVDACQPMEVIEKELQEIVLDCVK 246
            AAER GYG ERYEQ+EFQ +V + Y+ L+  S+W+++DA Q +E +EK+++EIVL+ V 
Sbjct: 187 KAAERGGYGGERYEQLEFQKKVAQHYK-LLEDSSWKMIDASQCIEDVEKQVEEIVLEHVV 246

Query: 247 KCQNGAPLSDLWS 260
            CQ G PLS LWS
Sbjct: 247 TCQKGKPLSSLWS 258

[Close]

BLAST of Csa4G006250 vs. TrEMBL
Match: A0A0D2QUS5_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_007G185700 PE=3 SV=1)

HSP 1 Score: 332.0 bits (850), Expect = 6.4e-88
Identity = 163/249 (65.46%), Postives = 197/249 (79.12%), Query Frame = 1


Query: 11  NFGVATTRISSSFRPKVVCKNFIRKIQMGTNQNSISRDSSSNSRGALVVLEGLDRCGKTT 70
           NFGV   R S +    + CK  +R I+M    N     +S+ SRGALVV EGLDR GKT+
Sbjct: 8   NFGVLAVRRSLNLGLNISCKRSVRSIRMENGNNCNLMGASNESRGALVVFEGLDRSGKTS 67

Query: 71  QASRLAQYLEGLGHSVELWRFPDRTTSVGQMISSYLSNESQLDDHTIHLLFSANRWEKRS 130
           Q  +L  YLEGLGHSVELWRFPDRTTSVGQMIS+YL+N+SQLDDHTIHLLFSANRWEKRS
Sbjct: 68  QCGKLLSYLEGLGHSVELWRFPDRTTSVGQMISAYLTNKSQLDDHTIHLLFSANRWEKRS 127

Query: 131 LMETKLKGGTTIILDRYSYSGVAFSCAKGLNFEWCKAPEVGLLAPDLVLYLNISPKMAAE 190
           +ME KLK GTT+I+DRYSYSGVAFS AKGL+FEWCKAPE GL+APDLV+YL+I P+ AAE
Sbjct: 128 MMEAKLKAGTTLIVDRYSYSGVAFSSAKGLDFEWCKAPENGLIAPDLVVYLDIPPEKAAE 187

Query: 191 RKGYGSERYEQIEFQNQVVEAYRDLMIGSNWQIVDACQPMEVIEKELQEIVLDCVKKCQN 250
           R GYG ERYEQ+EFQ +V + Y+ L+  S+W+++DA Q +E +EK+++EIVL+ V  CQ 
Sbjct: 188 RGGYGGERYEQLEFQKKVAQHYK-LLEDSSWKMIDASQCIEDVEKQVEEIVLEHVVTCQK 247

Query: 251 GAPLSDLWS 260
           G PLS LWS
Sbjct: 248 GKPLSSLWS 255

[Close]

BLAST of Csa4G006250 vs. TAIR10
Match: AT5G59440.3 (AT5G59440.3 P-loop containing nucleoside triphosphate hydrolases superfamily protein)

HSP 1 Score: 306.6 bits (784), Expect = 1.5e-83
Identity = 148/226 (65.49%), Postives = 181/226 (80.09%), Query Frame = 1


Query: 35  KIQMGTNQNSISRDSSSNSRGALVVLEGLDRCGKTTQASRLAQYLEGLGHSVELWRFPDR 94
           +++     + +  +SS   RGAL+VLEGLDR GK+TQ ++L  +L GLGH  ELWRFPDR
Sbjct: 47  RMESSNFSSGVRTESSVKPRGALIVLEGLDRSGKSTQCAKLLSFLNGLGHPTELWRFPDR 106

Query: 95  TTSVGQMISSYLSNESQLDDHTIHLLFSANRWEKRSLMETKLKGGTTIILDRYSYSGVAF 154
            TSVGQMIS+YLSN+SQLDDHTIHLLFSANRWEKRSLME KLK GTT+I+DRYSYSGVAF
Sbjct: 107 ETSVGQMISAYLSNKSQLDDHTIHLLFSANRWEKRSLMEEKLKTGTTLIVDRYSYSGVAF 166

Query: 155 SCAKGLNFEWCKAPEVGLLAPDLVLYLNISPKMAAERKGYGSERYEQIEFQNQVVEAYRD 214
           S AKGL+ EWCKAPE+GLLAPD VLYL+ISP+ AAER GYG ERYE++EFQ +V + Y+ 
Sbjct: 167 SSAKGLDIEWCKAPEIGLLAPDSVLYLDISPERAAERGGYGDERYERVEFQKKVADFYQT 226

Query: 215 LMIGSNWQIVDACQPMEVIEKELQEIVLDCVKKCQNGAPLSDLWSN 261
           L   S+W+I++A   ME +EK++Q++VLD VK+C  G PLS LWS+
Sbjct: 227 LG-DSSWKIINASDAMEEVEKKIQQVVLDQVKECTEGKPLSLLWSS 271

[Close]

BLAST of Csa4G006250 vs. NCBI nr
Match: gi|449468962|ref|XP_004152190.1| (PREDICTED: thymidylate kinase isoform X1 [Cucumis sativus])

HSP 1 Score: 525.0 bits (1351), Expect = 7.4e-146
Identity = 260/260 (100.00%), Postives = 260/260 (100.00%), Query Frame = 1


Query: 1   MLASTFFKPLNFGVATTRISSSFRPKVVCKNFIRKIQMGTNQNSISRDSSSNSRGALVVL 60
           MLASTFFKPLNFGVATTRISSSFRPKVVCKNFIRKIQMGTNQNSISRDSSSNSRGALVVL
Sbjct: 1   MLASTFFKPLNFGVATTRISSSFRPKVVCKNFIRKIQMGTNQNSISRDSSSNSRGALVVL 60

Query: 61  EGLDRCGKTTQASRLAQYLEGLGHSVELWRFPDRTTSVGQMISSYLSNESQLDDHTIHLL 120
           EGLDRCGKTTQASRLAQYLEGLGHSVELWRFPDRTTSVGQMISSYLSNESQLDDHTIHLL
Sbjct: 61  EGLDRCGKTTQASRLAQYLEGLGHSVELWRFPDRTTSVGQMISSYLSNESQLDDHTIHLL 120

Query: 121 FSANRWEKRSLMETKLKGGTTIILDRYSYSGVAFSCAKGLNFEWCKAPEVGLLAPDLVLY 180
           FSANRWEKRSLMETKLKGGTTIILDRYSYSGVAFSCAKGLNFEWCKAPEVGLLAPDLVLY
Sbjct: 121 FSANRWEKRSLMETKLKGGTTIILDRYSYSGVAFSCAKGLNFEWCKAPEVGLLAPDLVLY 180

Query: 181 LNISPKMAAERKGYGSERYEQIEFQNQVVEAYRDLMIGSNWQIVDACQPMEVIEKELQEI 240
           LNISPKMAAERKGYGSERYEQIEFQNQVVEAYRDLMIGSNWQIVDACQPMEVIEKELQEI
Sbjct: 181 LNISPKMAAERKGYGSERYEQIEFQNQVVEAYRDLMIGSNWQIVDACQPMEVIEKELQEI 240

Query: 241 VLDCVKKCQNGAPLSDLWSN 261
           VLDCVKKCQNGAPLSDLWSN
Sbjct: 241 VLDCVKKCQNGAPLSDLWSN 260

[Close]

BLAST of Csa4G006250 vs. NCBI nr
Match: gi|659108443|ref|XP_008454201.1| (PREDICTED: thymidylate kinase isoform X1 [Cucumis melo])

HSP 1 Score: 474.6 bits (1220), Expect = 1.1e-130
Identity = 231/260 (88.85%), Postives = 249/260 (95.77%), Query Frame = 1


Query: 1   MLASTFFKPLNFGVATTRISSSFRPKVVCKNFIRKIQMGTNQNSISRDSSSNSRGALVVL 60
           MLASTF+KPLNFGV TTR+SS FRPKVVCKNFIRKIQMGTNQNSISR SSSNSRGALVVL
Sbjct: 1   MLASTFYKPLNFGVVTTRLSSCFRPKVVCKNFIRKIQMGTNQNSISRGSSSNSRGALVVL 60

Query: 61  EGLDRCGKTTQASRLAQYLEGLGHSVELWRFPDRTTSVGQMISSYLSNESQLDDHTIHLL 120
           EGLDRCGKTTQ+SRL QYLEGLGHSVELWRFPDRTTSVGQMISSYLSNESQLDDHT+HLL
Sbjct: 61  EGLDRCGKTTQSSRLTQYLEGLGHSVELWRFPDRTTSVGQMISSYLSNESQLDDHTVHLL 120

Query: 121 FSANRWEKRSLMETKLKGGTTIILDRYSYSGVAFSCAKGLNFEWCKAPEVGLLAPDLVLY 180
           FSANRWEKRSLMETKLKGGTT+ILDRYSYSGVAFSCAKGL+ EWCKAPEVGL APDLVLY
Sbjct: 121 FSANRWEKRSLMETKLKGGTTLILDRYSYSGVAFSCAKGLDLEWCKAPEVGLPAPDLVLY 180

Query: 181 LNISPKMAAERKGYGSERYEQIEFQNQVVEAYRDLMIGSNWQIVDACQPMEVIEKELQEI 240
           L ISP+MAAER GYGSERYE+I+FQN+V+++Y+ +++GSNWQIVDAC+PMEVIEKELQE 
Sbjct: 181 LEISPEMAAERGGYGSERYERIDFQNKVIKSYQ-VLLGSNWQIVDACRPMEVIEKELQET 240

Query: 241 VLDCVKKCQNGAPLSDLWSN 261
           VLDCVKKCQNGAPLS LWSN
Sbjct: 241 VLDCVKKCQNGAPLSHLWSN 259

[Close]

BLAST of Csa4G006250 vs. NCBI nr
Match: gi|778689615|ref|XP_011652986.1| (PREDICTED: thymidylate kinase isoform X2 [Cucumis sativus])

HSP 1 Score: 453.0 bits (1164), Expect = 3.6e-124
Identity = 223/223 (100.00%), Postives = 223/223 (100.00%), Query Frame = 1


Query: 38  MGTNQNSISRDSSSNSRGALVVLEGLDRCGKTTQASRLAQYLEGLGHSVELWRFPDRTTS 97
           MGTNQNSISRDSSSNSRGALVVLEGLDRCGKTTQASRLAQYLEGLGHSVELWRFPDRTTS
Sbjct: 1   MGTNQNSISRDSSSNSRGALVVLEGLDRCGKTTQASRLAQYLEGLGHSVELWRFPDRTTS 60

Query: 98  VGQMISSYLSNESQLDDHTIHLLFSANRWEKRSLMETKLKGGTTIILDRYSYSGVAFSCA 157
           VGQMISSYLSNESQLDDHTIHLLFSANRWEKRSLMETKLKGGTTIILDRYSYSGVAFSCA
Sbjct: 61  VGQMISSYLSNESQLDDHTIHLLFSANRWEKRSLMETKLKGGTTIILDRYSYSGVAFSCA 120

Query: 158 KGLNFEWCKAPEVGLLAPDLVLYLNISPKMAAERKGYGSERYEQIEFQNQVVEAYRDLMI 217
           KGLNFEWCKAPEVGLLAPDLVLYLNISPKMAAERKGYGSERYEQIEFQNQVVEAYRDLMI
Sbjct: 121 KGLNFEWCKAPEVGLLAPDLVLYLNISPKMAAERKGYGSERYEQIEFQNQVVEAYRDLMI 180

Query: 218 GSNWQIVDACQPMEVIEKELQEIVLDCVKKCQNGAPLSDLWSN 261
           GSNWQIVDACQPMEVIEKELQEIVLDCVKKCQNGAPLSDLWSN
Sbjct: 181 GSNWQIVDACQPMEVIEKELQEIVLDCVKKCQNGAPLSDLWSN 223

[Close]

BLAST of Csa4G006250 vs. NCBI nr
Match: gi|659108449|ref|XP_008454204.1| (PREDICTED: thymidylate kinase isoform X2 [Cucumis melo])

HSP 1 Score: 407.9 bits (1047), Expect = 1.3e-110
Identity = 198/223 (88.79%), Postives = 214/223 (95.96%), Query Frame = 1


Query: 38  MGTNQNSISRDSSSNSRGALVVLEGLDRCGKTTQASRLAQYLEGLGHSVELWRFPDRTTS 97
           MGTNQNSISR SSSNSRGALVVLEGLDRCGKTTQ+SRL QYLEGLGHSVELWRFPDRTTS
Sbjct: 1   MGTNQNSISRGSSSNSRGALVVLEGLDRCGKTTQSSRLTQYLEGLGHSVELWRFPDRTTS 60

Query: 98  VGQMISSYLSNESQLDDHTIHLLFSANRWEKRSLMETKLKGGTTIILDRYSYSGVAFSCA 157
           VGQMISSYLSNESQLDDHT+HLLFSANRWEKRSLMETKLKGGTT+ILDRYSYSGVAFSCA
Sbjct: 61  VGQMISSYLSNESQLDDHTVHLLFSANRWEKRSLMETKLKGGTTLILDRYSYSGVAFSCA 120

Query: 158 KGLNFEWCKAPEVGLLAPDLVLYLNISPKMAAERKGYGSERYEQIEFQNQVVEAYRDLMI 217
           KGL+ EWCKAPEVGL APDLVLYL ISP+MAAER GYGSERYE+I+FQN+V+++Y+ +++
Sbjct: 121 KGLDLEWCKAPEVGLPAPDLVLYLEISPEMAAERGGYGSERYERIDFQNKVIKSYQ-VLL 180

Query: 218 GSNWQIVDACQPMEVIEKELQEIVLDCVKKCQNGAPLSDLWSN 261
           GSNWQIVDAC+PMEVIEKELQE VLDCVKKCQNGAPLS LWSN
Sbjct: 181 GSNWQIVDACRPMEVIEKELQETVLDCVKKCQNGAPLSHLWSN 222

[Close]

BLAST of Csa4G006250 vs. NCBI nr
Match: gi|1009166594|ref|XP_015901677.1| (PREDICTED: thymidylate kinase isoform X1 [Ziziphus jujuba])

HSP 1 Score: 361.7 bits (927), Expect = 1.1e-96
Identity = 179/256 (69.92%), Postives = 210/256 (82.03%), Query Frame = 1


Query: 5   TFFKPLNFGVATTRISSSFRPKVV-CKNFIRKIQMGTNQNSISRDSSSNSRGALVVLEGL 64
           T  K LNFGV   + S  F+  +  CK  IR IQM  N  S S  ++S+SRGALVVLEGL
Sbjct: 8   TVCKALNFGVVAVQRSLKFQLNIYRCKGSIRHIQMEINHKSNSSGANSDSRGALVVLEGL 67

Query: 65  DRCGKTTQASRLAQYLEGLGHSVELWRFPDRTTSVGQMISSYLSNESQLDDHTIHLLFSA 124
           DRCGKTTQ+ RL  YLEGLGH  ELWRFPDR+TSVGQMISSYLSN+SQLDDHT+HLLFSA
Sbjct: 68  DRCGKTTQSGRLLTYLEGLGHPAELWRFPDRSTSVGQMISSYLSNQSQLDDHTVHLLFSA 127

Query: 125 NRWEKRSLMETKLKGGTTIILDRYSYSGVAFSCAKGLNFEWCKAPEVGLLAPDLVLYLNI 184
           NRWEKRSLMETKLK GTT+I+DRYSYSGVAFS AKGL+FEWCKAPE+GLLAPD+VLYLNI
Sbjct: 128 NRWEKRSLMETKLKSGTTLIVDRYSYSGVAFSSAKGLDFEWCKAPEIGLLAPDVVLYLNI 187

Query: 185 SPKMAAERKGYGSERYEQIEFQNQVVEAYRDLMIGSNWQIVDACQPMEVIEKELQEIVLD 244
            P+ AAER GYG ERYE++EFQ +V ++Y++L   ++W+I+DA +P+E IEK+LQEIV D
Sbjct: 188 PPEKAAERGGYGGERYEKLEFQRKVAQSYQNLH-DASWKIIDASRPVEDIEKQLQEIVSD 247

Query: 245 CVKKCQNGAPLSDLWS 260
           C+K CQ G PLS LWS
Sbjct: 248 CIKTCQKGKPLSFLWS 262

The following BLAST results are available for this feature:

BLAST of Csa4G006250 vs. Swiss-Prot


Match Name	E-value	Identity	Description
ZEUS1_ARATH	2.6e-82	65.49	Thymidylate kinase OS=Arabidopsis thaliana GN=ZEU1 PE=2 SV=1	[more]
KTHY_MOUSE	1.7e-49	46.45	Thymidylate kinase OS=Mus musculus GN=Dtymk PE=1 SV=2	[more]
KTHY_HUMAN	2.9e-49	45.19	Thymidylate kinase OS=Homo sapiens GN=DTYMK PE=1 SV=4	[more]
KTHY_DICDI	5.1e-46	41.31	Thymidylate kinase OS=Dictyostelium discoideum GN=dtymk PE=3 SV=1	[more]
KTHY_SCHPO	8.7e-46	46.19	Thymidylate kinase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=tmp...	[more]

BLAST of Csa4G006250 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KTN6_CUCSA	5.2e-146	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_4G006250 PE=3 SV=1	[more]
A0A0D2SFC9_GOSRA	7.6e-89	65.22	Uncharacterized protein OS=Gossypium raimondii GN=B456_007G185700 PE=3 SV=1	[more]
A0A061EG90_THECC	7.6e-89	65.76	P-loop containing nucleoside triphosphate hydrolases superfamily protein OS=Theo...	[more]
A0A0B0N9J7_GOSAR	1.3e-88	64.82	Thymidylate kinase OS=Gossypium arboreum GN=F383_05248 PE=3 SV=1	[more]
A0A0D2QUS5_GOSRA	6.4e-88	65.46	Uncharacterized protein OS=Gossypium raimondii GN=B456_007G185700 PE=3 SV=1	[more]

BLAST of Csa4G006250 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G59440.3	1.5e-83	65.49	P-loop containing nucleoside triphosphate hydrolases superfamily pro...	[more]

BLAST of Csa4G006250 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449468962\|ref\|XP_004152190.1\|	7.4e-146	100.00	PREDICTED: thymidylate kinase isoform X1 [Cucumis sativus]	[more]
gi\|659108443\|ref\|XP_008454201.1\|	1.1e-130	88.85	PREDICTED: thymidylate kinase isoform X1 [Cucumis melo]	[more]
gi\|778689615\|ref\|XP_011652986.1\|	3.6e-124	100.00	PREDICTED: thymidylate kinase isoform X2 [Cucumis sativus]	[more]
gi\|659108449\|ref\|XP_008454204.1\|	1.3e-110	88.79	PREDICTED: thymidylate kinase isoform X2 [Cucumis melo]	[more]
gi\|1009166594\|ref\|XP_015901677.1\|	1.1e-96	69.92	PREDICTED: thymidylate kinase isoform X1 [Ziziphus jujuba]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR018094	Thymidylate_kinase
IPR018095	Thymidylate_kin_CS
IPR027417	P-loop_NTPase

Vocabulary: **Molecular Function**
Term	Definition
GO:0004798	thymidylate kinase activity
GO:0005524	ATP binding

Vocabulary: **Biological Process**
Term	Definition
GO:0006233	dTDP biosynthetic process

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006233	dTDP biosynthetic process
biological_process	GO:0046939	nucleotide phosphorylation
biological_process	GO:0006206	pyrimidine nucleobase metabolic process
biological_process	GO:0016310	phosphorylation
biological_process	GO:0009793	embryo development ending in seed dormancy
cellular_component	GO:0044444	cytoplasmic part
cellular_component	GO:0043231	intracellular membrane-bounded organelle
cellular_component	GO:0005829	cytosol
cellular_component	GO:0005739	mitochondrion
cellular_component	GO:0005654	nucleoplasm
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004798	thymidylate kinase activity
molecular_function	GO:0016787	hydrolase activity

Unigene

This gene is associated with the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
CU115843	cucumber EST collection version 3.0	transcribed_cluster
CU126293	cucumber EST collection version 3.0	transcribed_cluster

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Csa4G006250.1	Csa4G006250.1	mRNA

The following transcribed_cluster feature(s) are associated with this gene:


Feature Name	Unique Name	Type
CU115843	CU115843	transcribed_cluster
CU126293	CU126293	transcribed_cluster

InterPro

Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR018094	Thymidylate kinase	HAMAP	MF_00165	Thymidylate_kinase	coord: 56..245 score: 16
IPR018094	Thymidylate kinase	TIGRFAMs	TIGR00041	TIGR00041	coord: 54..236 score: 7.2
IPR018095	Thymidylate kinase, conserved site	PROSITE	PS01331	THYMIDYLATE_KINASE	coord: 143..155 scor
IPR027417	P-loop containing nucleoside triphosphate hydrolase	GENE3D	G3DSA:3.40.50.300		coord: 51..250 score: 3.7
IPR027417	P-loop containing nucleoside triphosphate hydrolase	unknown	SSF52540	P-loop containing nucleoside triphosphate hydrolases	coord: 54..247 score: 1.58
None	No IPR available	PANTHER	PTHR10344	THYMIDYLATE KINASE	coord: 41..258 score: 1.4E
None	No IPR available	PFAM	PF02223	Thymidylate_kin	coord: 60..236 score: 5.9

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Csa4G006250	Cla012732	Watermelon (97103) v1	cuwmB304
Csa4G006250	MELO3C017855	Melon (DHL92) v3.5.1	cumeB312
Csa4G006250	ClCG07G012360	Watermelon (Charleston Gray)	cuwcgB326
Csa4G006250	Lsi07G004340	Bottle gourd (USVL1VR-Ls)	culsiB312
Csa4G006250	MELO3C017855.2	Melon (DHL92) v3.6.1	cumedB297
Csa4G006250	Cla97C07G139150	Watermelon (97103) v2	cuwmbB323
Csa4G006250	Bhi01G002787	Wax gourd	cuwgoB366
Csa4G006250	CSPI04G01810	Wild cucumber (PI 183967)	cpicuB182
Csa4G006250	Cucsa.096180	Cucumber (Gy14) v1	cgycuB102
Csa4G006250	CmaCh01G001680	Cucurbita maxima (Rimu)	cmacuB477
Csa4G006250	CmoCh01G001750	Cucurbita moschata (Rifu)	cmocuB466
Csa4G006250	Cp4.1LG02g15150	Cucurbita pepo (Zucchini)	cpecuB568
Csa4G006250	CsGy4G001730	Cucumber (Gy14) v2	cgybcuB165
Csa4G006250	Carg04209	Silver-seed gourd	carcuB0225

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Csa4G006250	Melon (DHL92) v3.6.1	cumedB269
Csa4G006250	Silver-seed gourd	carcuB0128
Csa4G006250	Silver-seed gourd	carcuB0226
Csa4G006250	Watermelon (97103) v2	cuwmbB330
Csa4G006250	Cucumber (Gy14) v1	cgycuB124
Csa4G006250	Cucurbita maxima (Rimu)	cmacuB260
Csa4G006250	Cucurbita maxima (Rimu)	cmacuB466
Csa4G006250	Cucurbita moschata (Rifu)	cmocuB245
Csa4G006250	Cucurbita moschata (Rifu)	cmocuB456
Csa4G006250	Melon (DHL92) v3.5.1	cumeB285
Csa4G006250	Watermelon (Charleston Gray)	cuwcgB267
Csa4G006250	Cucurbita pepo (Zucchini)	cpecuB575
Csa4G006250	Cucurbita pepo (Zucchini)	cpecuB615
Csa4G006250	Cucurbita pepo (Zucchini)	cpecuB620
Csa4G006250	Bottle gourd (USVL1VR-Ls)	culsiB268
Csa4G006250	Cucumber (Gy14) v2	cgybcuB317