Csa4G001540 (gene) Cucumber (Chinese Long) v2

Overview
NameCsa4G001540
Typegene
OrganismCucumis. sativus (Cucumber (Chinese Long) v2)
DescriptionBifunctional protein folD; contains IPR000672 (Tetrahydrofolate dehydrogenase/cyclohydrolase)
LocationChr4 : 298375 .. 301164 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TAGCTTTAGAAAGTCACGCTCATCGTAATTACCCAAAACAAATGACTTAGATTTGGAATCCTTTCTCTTCGGCTGAAGAAAATGGGCCACACAAAATTGTGCACGGACGTACCCATTTCTCGCCAAACACAGTCAGATTGCCCAAATCCAAGCCCATCCACCTATTCCCATGCCTGCATCAACTCACTGATTATACACAATCAATCCTTCTCAATTTCAGCTTCTCTATTACATGCCATTGCCACTGGCAGCTGCAAATTCAATTCGGAAGTAGTAACCATTTTCTTCTTCTCACCTGCAAAAATGGCGTCCGAATCCGACCACAAGGCTACAATCATCGACGGCAAAAAGATTGCACAAACTGTCAGATCTGAAATCACGGAGGAAGTGAACAAACTCTCTCAGAAATATGGAAAGGTTTAGTTTCCTTTGCTCCAGTCAAATTCTTACAATTTCCATCAACTCTTTTGAGATATTATGTTTTATATCTATCTGATTTTAGCATTTTTCTTGTTTCATATGGTGTGGGATTTGTGAAGATTCCAGGACTGGCGGTGGTGATTGTGGGGAATAGAAAAGATTCGCTTACCTATGTAAATATGAAGAGGAAGGCGTGCCTGGAAGTAGGGATCAAGTCTTTTGAGATTGATCTTCCTGAGCAAGTGTCTGAGGCTGAATTGATCAGCAAAGTTCACGAGCTTAATGCCAATCCTGAAGTGCATGGTTAATCTCTTCTTTACTTTTATGCTCAGGATTTCTTCTAATTTTAGTGCTGTAGTTCAAATTCGATGGAGTCTCCACTGTTTTTGGAATAGTACAATGAAGATGCATTTCTGATATATAATTGTTCTTTGATTCTTTTTACCTTTCTAAAGCAATGTGATTCATTGAAGAAAAAGCACCCTTTTAGTGCTTCTAGAGTGATTTAAAATATTATAAGTGTTTATAGTAACTTTCAAAGTTATTTTCTGTGTCAATTTGTTTTTTTTTTAAGAAAAAAGTTCTTCAAATGGCTCAAAAGTACTTTTTCAGAGTTTTAAAGGTACCAATTCGTGCATAACTCTGTTGATAATAATATCAATTACCATCTCTAAAGTTGTTGAACTCAAAAGTAATTTACAATTTTTGAAGAACTTTGTTTCTTTGACAGGGATATTGGTTCAGCTTCCTTTGCCGAACCACATAAATGAAGAGAAAGTTCTATCTGAAATTAGCATTGAGAAGGATGTAGATGGCTTTCATCCTCTGAACATTGGGAAGCTTGCAATGAAAGGCAGAGATCCCTTGTTCCTACCTTGCACTCCGAAGGCAATAATGAATGTTTTATTAGCCTCAGAAAATCATTCAACTCATTCTTGATAGATCTCTTTTAGCTAGAAGAGCAATATATCCTTGTTTATCTATGTTCAATATTTGGTTTCATGCATATCTTAGCCCACTGAGATATCGTTTTATGTGCAGGGGTGTATTGAACTGCTATCGCGAAGTGGAATAAGCATCAGAGGCAAGAAAGCAGTTGTAATGGGGCGAAGCAACATTGTTGGATTACCAGTTTCATTGTTGCTTCTTAAAGCAGATGCAACTGTGACAATCGTCCATTCACGTTCCGTCGATCCAGAAAGTGTTATCCGCGAAGCAGACATTATAATTGCTGCAGCAGGACAGGCACAAATGGTATAATTTGGCTATACAACCTCAGTGTTTTCTCTTTCCCCTTTACTTTTATCTGAATATCAGCATTTGTCTCTAAACTTAGGGAATATGTATAGGTGCTAGAATAGTCCTGGAAACGAAAAAAGGTCAGATCCATAGGTATAATTAAAACTTTTCTTAGAAATTTTAAATTTTATTCTACATAGTGCATGTTGTTAACTAAACGATGACACATCTTCAATGATTTGATGCAAAGTGGTCAGCAAAATTTTCTTATATCATCTCTCTCTCTCTTTGTGTTATTGGAATCCGCCCATTTTTCCCCCAATAAGCATCTACCTCATTATTCAGTCAACAATGGTAAATCAAACAAGTTAGGGAAAAATATTTGAGAGAATTAAGCAAAAGCACAATCATCAAAGTTGGTACTTCAAGCAATACTTTGCCATTTGCATATGCATGATAGAACAATAACTGCAAAAGCATTAAGAAACAGGACTGTAGGGAATATGATTTAGTTGACTTGTTGAAACATCCTAGTTTTGAGACTAACTTGTGCACATGGTGATTTCGTTTGGTTGATGTTAGATCAAGGGGAGTTGGATTAAACCAGGTGCTGCAGTTATCGATGTCGGTACAAATGCAGTTGATGATCCAACCAAGAAGTCAGGCTACCGGCTGGTCGGTGATGTAGATTTCCAGGAAGCTTGTAAAGTAGCTGGTTGGATAACTCCGGTTCCGGGTGGCGTAGGTCCTATGACCGTTGCCATGCTGCTTAGGAATACTCTGGATGGGGCTAAGCGTGTGATCGAGCAGTGAAAAAGATACAGCCAGCTTGTTACCGATCCTCGTGGGACTTTTTCGGAGTAATTTCAAAATAAATGGTTCGTTTGTTTTGTGCTATCTTTTTTTTGTACTAATAATAGTTGATTTTTCATTGAAAATAATGGAGTAATTAAAATCAAATCATAACACCCCAATCTTGAAAAATCTAAGATAAGTTTTCTTAAAGTACCAACATAAATCATCAATAATCATGGTGTATGATTTGATTTGATTTGATTTCCCTGTAATCTAGAAAAGCATTTGAGGTGGAAGTTATGAGTTCACAAAATGAGAGAAGCTGTTGATTTTGCA

mRNA sequence

ATGGGCCACACAAAATTGTGCACGGACGTACCCATTTCTCGCCAAACACAGTCAGATTGCCCAAATCCAAGCCCATCCACCTATTCCCATGCCTGCATCAACTCACTGATTATACACAATCAATCCTTCTCAATTTCAGCTTCTCTATTACATGCCATTGCCACTGGCAGCTGCAAATTCAATTCGGAAGTAGTAACCATTTTCTTCTTCTCACCTGCAAAAATGGCGTCCGAATCCGACCACAAGGCTACAATCATCGACGGCAAAAAGATTGCACAAACTGTCAGATCTGAAATCACGGAGGAAGTGAACAAACTCTCTCAGAAATATGGAAAGATTCCAGGACTGGCGGTGGTGATTGTGGGGAATAGAAAAGATTCGCTTACCTATGTAAATATGAAGAGGAAGGCGTGCCTGGAAGTAGGGATCAAGTCTTTTGAGATTGATCTTCCTGAGCAAGTGTCTGAGGCTGAATTGATCAGCAAAGTTCACGAGCTTAATGCCAATCCTGAAGTGCATGGGATATTGGTTCAGCTTCCTTTGCCGAACCACATAAATGAAGAGAAAGTTCTATCTGAAATTAGCATTGAGAAGGATGTAGATGGCTTTCATCCTCTGAACATTGGGAAGCTTGCAATGAAAGGCAGAGATCCCTTGTTCCTACCTTGCACTCCGAAGGGGTGTATTGAACTGCTATCGCGAAGTGGAATAAGCATCAGAGGCAAGAAAGCAGTTGTAATGGGGCGAAGCAACATTGTTGGATTACCAGTTTCATTGTTGCTTCTTAAAGCAGATGCAACTGTGACAATCGTCCATTCACGTTCCGTCGATCCAGAAAGTGTTATCCGCGAAGCAGACATTATAATTGCTGCAGCAGGACAGGCACAAATGATCAAGGGGAGTTGGATTAAACCAGGTGCTGCAGTTATCGATGTCGGTACAAATGCAGTTGATGATCCAACCAAGAAGTCAGGCTACCGGCTGGTCGGTGATGTAGATTTCCAGGAAGCTTGTAAAGTAGCTGGTTGGATAACTCCGGTTCCGGGTGGCGTAGGTCCTATGACCGTTGCCATGCTGCTTAGGAATACTCTGGATGGGGCTAAGCGTGTGATCGAGCAGTGA

Coding sequence (CDS)

ATGGGCCACACAAAATTGTGCACGGACGTACCCATTTCTCGCCAAACACAGTCAGATTGCCCAAATCCAAGCCCATCCACCTATTCCCATGCCTGCATCAACTCACTGATTATACACAATCAATCCTTCTCAATTTCAGCTTCTCTATTACATGCCATTGCCACTGGCAGCTGCAAATTCAATTCGGAAGTAGTAACCATTTTCTTCTTCTCACCTGCAAAAATGGCGTCCGAATCCGACCACAAGGCTACAATCATCGACGGCAAAAAGATTGCACAAACTGTCAGATCTGAAATCACGGAGGAAGTGAACAAACTCTCTCAGAAATATGGAAAGATTCCAGGACTGGCGGTGGTGATTGTGGGGAATAGAAAAGATTCGCTTACCTATGTAAATATGAAGAGGAAGGCGTGCCTGGAAGTAGGGATCAAGTCTTTTGAGATTGATCTTCCTGAGCAAGTGTCTGAGGCTGAATTGATCAGCAAAGTTCACGAGCTTAATGCCAATCCTGAAGTGCATGGGATATTGGTTCAGCTTCCTTTGCCGAACCACATAAATGAAGAGAAAGTTCTATCTGAAATTAGCATTGAGAAGGATGTAGATGGCTTTCATCCTCTGAACATTGGGAAGCTTGCAATGAAAGGCAGAGATCCCTTGTTCCTACCTTGCACTCCGAAGGGGTGTATTGAACTGCTATCGCGAAGTGGAATAAGCATCAGAGGCAAGAAAGCAGTTGTAATGGGGCGAAGCAACATTGTTGGATTACCAGTTTCATTGTTGCTTCTTAAAGCAGATGCAACTGTGACAATCGTCCATTCACGTTCCGTCGATCCAGAAAGTGTTATCCGCGAAGCAGACATTATAATTGCTGCAGCAGGACAGGCACAAATGATCAAGGGGAGTTGGATTAAACCAGGTGCTGCAGTTATCGATGTCGGTACAAATGCAGTTGATGATCCAACCAAGAAGTCAGGCTACCGGCTGGTCGGTGATGTAGATTTCCAGGAAGCTTGTAAAGTAGCTGGTTGGATAACTCCGGTTCCGGGTGGCGTAGGTCCTATGACCGTTGCCATGCTGCTTAGGAATACTCTGGATGGGGCTAAGCGTGTGATCGAGCAGTGA

Protein sequence

MGHTKLCTDVPISRQTQSDCPNPSPSTYSHACINSLIIHNQSFSISASLLHAIATGSCKFNSEVVTIFFFSPAKMASESDHKATIIDGKKIAQTVRSEITEEVNKLSQKYGKIPGLAVVIVGNRKDSLTYVNMKRKACLEVGIKSFEIDLPEQVSEAELISKVHELNANPEVHGILVQLPLPNHINEEKVLSEISIEKDVDGFHPLNIGKLAMKGRDPLFLPCTPKGCIELLSRSGISIRGKKAVVMGRSNIVGLPVSLLLLKADATVTIVHSRSVDPESVIREADIIIAAAGQAQMIKGSWIKPGAAVIDVGTNAVDDPTKKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMTVAMLLRNTLDGAKRVIEQ*
Homology
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BLAST of Csa4G001540 vs. Swiss-Prot
Match: FOLD2_ARATH (Bifunctional protein FolD 2 OS=Arabidopsis thaliana GN=FOLD2 PE=2 SV=1)

HSP 1 Score: 491.1 bits (1263), Expect = 1.1e-137
Identity = 242/296 (81.76%), Postives = 269/296 (90.88%), Query Frame = 1 

Query: 75  MASESDHKATIIDGKKIAQTVRSEITEEVNKLSQKYGKIPGLAVVIVGNRKDSLTYVNMK 134
           MAS SDH A IIDGK IA T+RSEI EEV  LS+K+GK+PGLAVVIVG+RKDS TYVN K
Sbjct: 1   MASSSDHTAKIIDGKAIAHTIRSEIAEEVRGLSEKHGKVPGLAVVIVGSRKDSQTYVNTK 60

Query: 135 RKACLEVGIKSFEIDLPEQVSEAELISKVHELNANPEVHGILVQLPLPNHINEEKVLSEI 194
           RKAC EVGIKSF++ LPE+VSEA+LISKVHELN+NP+VHGILVQLPLP HINEE +L  I
Sbjct: 61  RKACAEVGIKSFDVGLPEEVSEADLISKVHELNSNPDVHGILVQLPLPKHINEEHILGAI 120

Query: 195 SIEKDVDGFHPLNIGKLAMKGRDPLFLPCTPKGCIELLSRSGISIRGKKAVVMGRSNIVG 254
           SI+KDVDGFHPLNIGKLAMKGR+PLFLPCTPKGC+ELL+RSG+ I+G++AVV+GRSNIVG
Sbjct: 121 SIDKDVDGFHPLNIGKLAMKGREPLFLPCTPKGCLELLARSGVKIKGQRAVVVGRSNIVG 180

Query: 255 LPVSLLLLKADATVTIVHSRSVDPESVIREADIIIAAAGQAQMIKGSWIKPGAAVIDVGT 314
           LPVSLLLLKADATVT VHS + DPE++IREADI+IAA GQA MIKG+WIKPGAAVIDVGT
Sbjct: 181 LPVSLLLLKADATVTTVHSHTKDPEAIIREADIVIAACGQAHMIKGNWIKPGAAVIDVGT 240

Query: 315 NAVDDPTKKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMTVAMLLRNTLDGAKRV 371
           NAV DP+KKSGYRLVGDVDF EA KVAG+ITPVPGGVGPMTVAMLLRNT+DGAKRV
Sbjct: 241 NAVSDPSKKSGYRLVGDVDFAEASKVAGFITPVPGGVGPMTVAMLLRNTVDGAKRV 296

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BLAST of Csa4G001540 vs. Swiss-Prot
Match: FOLD4_ARATH (Bifunctional protein FolD 4, chloroplastic OS=Arabidopsis thaliana GN=FOLD4 PE=1 SV=1)

HSP 1 Score: 389.0 bits (998), Expect = 5.7e-107
Identity = 186/294 (63.27%), Postives = 236/294 (80.27%), Query Frame = 1 

Query: 77  SESDHKATIIDGKKIAQTVRSEITEEVNKLSQKYGKIPGLAVVIVGNRKDSLTYVNMKRK 136
           ++S+  A +IDGK +A+ +R EIT EV+++ +  G IPGLAV++VG+RKDS TYV  K+K
Sbjct: 63  TKSEGGAIVIDGKAVAKKIRDEITIEVSRMKESIGVIPGLAVILVGDRKDSATYVRNKKK 122

Query: 137 ACLEVGIKSFEIDLPEQVSEAELISKVHELNANPEVHGILVQLPLPNHINEEKVLSEISI 196
           AC  VGIKSFE+ L E  SE E++  V   N +P VHGILVQLPLP+H++E+ +L+ +SI
Sbjct: 123 ACDSVGIKSFEVRLAEDSSEEEVLKSVSGFNDDPSVHGILVQLPLPSHMDEQNILNAVSI 182

Query: 197 EKDVDGFHPLNIGKLAMKGRDPLFLPCTPKGCIELLSRSGISIRGKKAVVMGRSNIVGLP 256
           EKDVDGFHPLNIG+LAM+GR+PLF+PCTPKGCIELL R  I I+GK+AVV+GRSNIVG+P
Sbjct: 183 EKDVDGFHPLNIGRLAMRGREPLFVPCTPKGCIELLHRYNIEIKGKRAVVIGRSNIVGMP 242

Query: 257 VSLLLLKADATVTIVHSRSVDPESVIREADIIIAAAGQAQMIKGSWIKPGAAVIDVGTNA 316
            +LLL + DATV+I+HSR+ +PE + READIII+A GQ  M++GSWIKPGA +IDVG N 
Sbjct: 243 AALLLQREDATVSIIHSRTKNPEEITREADIIISAVGQPNMVRGSWIKPGAVLIDVGINP 302

Query: 317 VDDPTKKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMTVAMLLRNTLDGAKRV 371
           V+DP+   GYRLVGD+ ++EA KVA  ITPVPGGVGPMT+AMLL NTL  AKR+
Sbjct: 303 VEDPSAARGYRLVGDICYEEASKVASAITPVPGGVGPMTIAMLLSNTLTSAKRI 356

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BLAST of Csa4G001540 vs. Swiss-Prot
Match: FOLD1_ARATH (Bifunctional protein FolD 1, mitochondrial OS=Arabidopsis thaliana GN=FOLD1 PE=2 SV=1)

HSP 1 Score: 366.3 bits (939), Expect = 4.0e-100
Identity = 175/299 (58.53%), Postives = 229/299 (76.59%), Query Frame = 1 

Query: 72  PAKMASESDHKATIIDGKKIAQTVRSEITEEVNKLSQKYGKIPGLAVVIVGNRKDSLTYV 131
           P  ++ E++ K  +IDG  IA+ +R++I  EV K+ +  GK+PGLAVV+VG ++DS TYV
Sbjct: 52  PPPVSFETEQKTVVIDGNVIAEEIRTKIISEVGKMKKAVGKVPGLAVVLVGEQRDSQTYV 111

Query: 132 NMKRKACLEVGIKSFEIDLPEQVSEAELISKVHELNANPEVHGILVQLPLPNHINEEKVL 191
             K KAC E GIKS   +LPE  +E ++IS + + N +  +HGILVQLPLP H+NE K+L
Sbjct: 112 RNKIKACEETGIKSVLAELPEDCTEGQIISVLRKFNEDTSIHGILVQLPLPQHLNESKIL 171

Query: 192 SEISIEKDVDGFHPLNIGKLAMKGRDPLFLPCTPKGCIELLSRSGISIRGKKAVVMGRSN 251
           + + +EKDVDGFHPLN+G LAM+GR+PLF+ CTPKGC+ELL R+G+ I GK AVV+GRSN
Sbjct: 172 NMVRLEKDVDGFHPLNVGNLAMRGREPLFVSCTPKGCVELLIRTGVEIAGKNAVVIGRSN 231

Query: 252 IVGLPVSLLLLKADATVTIVHSRSVDPESVIREADIIIAAAGQAQMIKGSWIKPGAAVID 311
           IVGLP+SLLL + DATV+ VH+ + DPE + R+ADI+IAAAG   +++GSW+KPGA VID
Sbjct: 232 IVGLPMSLLLQRHDATVSTVHAFTKDPEHITRKADIVIAAAGIPNLVRGSWLKPGAVVID 291

Query: 312 VGTNAVDDPTKKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMTVAMLLRNTLDGAKRV 371
           VGT  V+D + + GYRLVGDV ++EA  VA  ITPVPGGVGPMT+ MLL NTL+ AKR+
Sbjct: 292 VGTTPVEDSSCEFGYRLVGDVCYEEALGVASAITPVPGGVGPMTITMLLCNTLEAAKRI 350

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BLAST of Csa4G001540 vs. Swiss-Prot
Match: FOLD_MOOTA (Bifunctional protein FolD OS=Moorella thermoacetica (strain ATCC 39073) GN=folD PE=3 SV=1)

HSP 1 Score: 302.8 bits (774), Expect = 5.4e-81
Identity = 160/287 (55.75%), Postives = 205/287 (71.43%), Query Frame = 1 

Query: 83  ATIIDGKKIAQTVRSEITEEVNKLSQKYGKIPGLAVVIVGNRKDSLTYVNMKRKACLEVG 142
           A I+DGKKIA  VR+E+ EEV++L  + G  PGLAVV+VG    S  YV  K +AC EVG
Sbjct: 3   AQILDGKKIAAEVRAEVKEEVSRLKAE-GINPGLAVVLVGEDPASQVYVRNKHRACEEVG 62

Query: 143 IKSFEIDLPEQVSEAELISKVHELNANPEVHGILVQLPLPNHINEEKVLSEISIEKDVDG 202
           I S    LP   S+AEL+  + +LN +P++HGILVQLPLP+HI+E+KV+  I++EKDVDG
Sbjct: 63  IYSEVHRLPAATSQAELLKLIDQLNKDPKIHGILVQLPLPDHIDEKKVIDAIALEKDVDG 122

Query: 203 FHPLNIGKLAMKGRDPLFLPCTPKGCIELLSRSGISIRGKKAVVMGRSNIVGLPVSLLLL 262
           F P N+G L +   D  F PCTP GC+ LL ++GI  +GKKAVV+GRSNIVG PV+++LL
Sbjct: 123 FSPANVGNLVIG--DKCFYPCTPHGCMVLLEKAGIDPKGKKAVVVGRSNIVGKPVAMMLL 182

Query: 263 KADATVTIVHSRSVDPESVIREADIIIAAAGQAQMIKGSWIKPGAAVIDVGTNAVDDPTK 322
              ATVTI HSR+ D  +  R+ADI+IAA G+ ++I G  IK GA VIDVG N V +   
Sbjct: 183 ARHATVTICHSRTRDLAAECRQADILIAAVGKPELITGDMIKEGAVVIDVGINRVGEK-- 242

Query: 323 KSGYRLVGDVDFQEACKVAGWITPVPGGVGPMTVAMLLRNTLDGAKR 370
               +LVGDV F+ A + AGWITPVPGGVGPMT+AMLL+NT++ A+R
Sbjct: 243 ----KLVGDVHFESAAQKAGWITPVPGGVGPMTIAMLLKNTVEAARR 280

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BLAST of Csa4G001540 vs. Swiss-Prot
Match: FOLD_LISMC (Bifunctional protein FolD OS=Listeria monocytogenes serotype 4b (strain CLIP80459) GN=folD PE=3 SV=1)

HSP 1 Score: 301.6 bits (771), Expect = 1.2e-80
Identity = 160/286 (55.94%), Postives = 205/286 (71.68%), Query Frame = 1 

Query: 85  IIDGKKIAQTVRSEITEEVNKLSQKYGKIPGLAVVIVGNRKDSLTYVNMKRKACLEVGIK 144
           IIDGKK+A+ ++ ++T EV +L  K GK PGLAVV+VG+ + S TYV  K+K   E G+K
Sbjct: 4   IIDGKKLAKEIQEKVTREVAELV-KEGKKPGLAVVLVGDNQASRTYVRNKQKRTEEAGMK 63

Query: 145 SFEIDLPEQVSEAELISKVHELNANPEVHGILVQLPLPNHINEEKVLSEISIEKDVDGFH 204
           S  I+LPE V+E +L+S V ELN +  +HGILVQLPLP HI+EEKV+  IS +KDVDGFH
Sbjct: 64  SVLIELPENVTEEKLLSVVEELNEDKTIHGILVQLPLPEHISEEKVIDTISYDKDVDGFH 123

Query: 205 PLNIGKLAMKGRDPLFLPCTPKGCIELLSRSGISIRGKKAVVMGRSNIVGLPVSLLLLKA 264
           P+N+G L + G+D  F+PCTP G IEL+  +G  I GK+AVV+GRSNIVG PV+ LLL  
Sbjct: 124 PVNVGNLFI-GKDS-FVPCTPAGIIELIKSTGTQIEGKRAVVIGRSNIVGKPVAQLLLNE 183

Query: 265 DATVTIVHSRSVDPESVIREADIIIAAAGQAQMIKGSWIKPGAAVIDVGTNAVDDPTKKS 324
           +ATVTI HSR+ D   V +EADI++ A G A+ +K  +IKPGA VIDVG +      +  
Sbjct: 184 NATVTIAHSRTKDLPQVAKEADILVVATGLAKFVKKDYIKPGAVVIDVGMD------RDE 243

Query: 325 GYRLVGDVDFQEACKVAGWITPVPGGVGPMTVAMLLRNTLDGAKRV 371
             +L GDVDF +  + AG+ITPVPGGVGPMT+ MLL NTL  AKR+
Sbjct: 244 NNKLCGDVDFDDVVEEAGFITPVPGGVGPMTITMLLANTLKAAKRI 280

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BLAST of Csa4G001540 vs. TrEMBL
Match: A0A0A0KWH1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G001540 PE=3 SV=1)

HSP 1 Score: 735.7 bits (1898), Expect = 2.8e-209
Identity = 373/373 (100.00%), Postives = 373/373 (100.00%), Query Frame = 1 

Query: 1   MGHTKLCTDVPISRQTQSDCPNPSPSTYSHACINSLIIHNQSFSISASLLHAIATGSCKF 60
           MGHTKLCTDVPISRQTQSDCPNPSPSTYSHACINSLIIHNQSFSISASLLHAIATGSCKF
Sbjct: 1   MGHTKLCTDVPISRQTQSDCPNPSPSTYSHACINSLIIHNQSFSISASLLHAIATGSCKF 60

Query: 61  NSEVVTIFFFSPAKMASESDHKATIIDGKKIAQTVRSEITEEVNKLSQKYGKIPGLAVVI 120
           NSEVVTIFFFSPAKMASESDHKATIIDGKKIAQTVRSEITEEVNKLSQKYGKIPGLAVVI
Sbjct: 61  NSEVVTIFFFSPAKMASESDHKATIIDGKKIAQTVRSEITEEVNKLSQKYGKIPGLAVVI 120

Query: 121 VGNRKDSLTYVNMKRKACLEVGIKSFEIDLPEQVSEAELISKVHELNANPEVHGILVQLP 180
           VGNRKDSLTYVNMKRKACLEVGIKSFEIDLPEQVSEAELISKVHELNANPEVHGILVQLP
Sbjct: 121 VGNRKDSLTYVNMKRKACLEVGIKSFEIDLPEQVSEAELISKVHELNANPEVHGILVQLP 180

Query: 181 LPNHINEEKVLSEISIEKDVDGFHPLNIGKLAMKGRDPLFLPCTPKGCIELLSRSGISIR 240
           LPNHINEEKVLSEISIEKDVDGFHPLNIGKLAMKGRDPLFLPCTPKGCIELLSRSGISIR
Sbjct: 181 LPNHINEEKVLSEISIEKDVDGFHPLNIGKLAMKGRDPLFLPCTPKGCIELLSRSGISIR 240

Query: 241 GKKAVVMGRSNIVGLPVSLLLLKADATVTIVHSRSVDPESVIREADIIIAAAGQAQMIKG 300
           GKKAVVMGRSNIVGLPVSLLLLKADATVTIVHSRSVDPESVIREADIIIAAAGQAQMIKG
Sbjct: 241 GKKAVVMGRSNIVGLPVSLLLLKADATVTIVHSRSVDPESVIREADIIIAAAGQAQMIKG 300

Query: 301 SWIKPGAAVIDVGTNAVDDPTKKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMTVAMLL 360
           SWIKPGAAVIDVGTNAVDDPTKKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMTVAMLL
Sbjct: 301 SWIKPGAAVIDVGTNAVDDPTKKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMTVAMLL 360

Query: 361 RNTLDGAKRVIEQ 374
           RNTLDGAKRVIEQ
Sbjct: 361 RNTLDGAKRVIEQ 373

[Close]
BLAST of Csa4G001540 vs. TrEMBL
Match: M5VKQ0_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa009269mg PE=3 SV=1)

HSP 1 Score: 523.5 bits (1347), Expect = 2.2e-145
Identity = 259/299 (86.62%), Postives = 281/299 (93.98%), Query Frame = 1 

Query: 75  MASESDHKATIIDGKKIAQTVRSEITEEVNKLSQKYGKIPGLAVVIVGNRKDSLTYVNMK 134
           MAS+SDHKA IIDGK IAQT+R+EI EEV  LSQKYGK+PGLAVVIVGNRKDS +YV+MK
Sbjct: 1   MASQSDHKAAIIDGKAIAQTIRNEIAEEVRHLSQKYGKVPGLAVVIVGNRKDSQSYVSMK 60

Query: 135 RKACLEVGIKSFEIDLPEQVSEAELISKVHELNANPEVHGILVQLPLPNHINEEKVLSEI 194
           RKAC EVGI S +IDLPE VS+ +LI+KVHELNANP+VHGILVQLPLP HINEEKVLSEI
Sbjct: 61  RKACAEVGILSLDIDLPEDVSQVDLIAKVHELNANPDVHGILVQLPLPKHINEEKVLSEI 120

Query: 195 SIEKDVDGFHPLNIGKLAMKGRDPLFLPCTPKGCIELLSRSGISIRGKKAVVMGRSNIVG 254
           SIEKDVDGFHPLNIGKLAMKGR+PLFLPCTPKGC+ELLSRSGISI+GKKAVV+GRSNIVG
Sbjct: 121 SIEKDVDGFHPLNIGKLAMKGREPLFLPCTPKGCLELLSRSGISIKGKKAVVVGRSNIVG 180

Query: 255 LPVSLLLLKADATVTIVHSRSVDPESVIREADIIIAAAGQAQMIKGSWIKPGAAVIDVGT 314
           LPVSLLLLKADATVT+VHS S DPES+IREADI+IAAAGQA MIKGSWIKPGAAVIDVGT
Sbjct: 181 LPVSLLLLKADATVTVVHSHSHDPESIIREADIVIAAAGQAMMIKGSWIKPGAAVIDVGT 240

Query: 315 NAVDDPTKKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMTVAMLLRNTLDGAKRVIEQ 374
           NA+DD ++KSGYRLVGDVDFQEACKVAGW+TPVPGGVGPMTVAMLL NTLDGAKRVI Q
Sbjct: 241 NAIDDSSRKSGYRLVGDVDFQEACKVAGWVTPVPGGVGPMTVAMLLNNTLDGAKRVIAQ 299

[Close]
BLAST of Csa4G001540 vs. TrEMBL
Match: B9T0V4_RICCO (Methylenetetrahydrofolate dehydrogenase, putative OS=Ricinus communis GN=RCOM_1439680 PE=3 SV=1)

HSP 1 Score: 520.0 bits (1338), Expect = 2.4e-144
Identity = 260/299 (86.96%), Postives = 282/299 (94.31%), Query Frame = 1 

Query: 75  MASESDHKATIIDGKKIAQTVRSEITEEVNKLSQKYGKIPGLAVVIVGNRKDSLTYVNMK 134
           MA+ SDHKATIIDGK IAQT+RSEI +EV +LS+KYGK+PGLAVVIVGNRKDS +YV+MK
Sbjct: 1   MAAPSDHKATIIDGKAIAQTIRSEIADEVRQLSEKYGKVPGLAVVIVGNRKDSQSYVSMK 60

Query: 135 RKACLEVGIKSFEIDLPEQVSEAELISKVHELNANPEVHGILVQLPLPNHINEEKVLSEI 194
           RKAC EVGIKSF+I+LPEQVSEAELISKVHELNAN +VHGILVQLPLP HINEEKVLSEI
Sbjct: 61  RKACAEVGIKSFDINLPEQVSEAELISKVHELNANTDVHGILVQLPLPKHINEEKVLSEI 120

Query: 195 SIEKDVDGFHPLNIGKLAMKGRDPLFLPCTPKGCIELLSRSGISIRGKKAVVMGRSNIVG 254
           SIEKDVDGFHPLNIGKLAMKGR+PLFLPCTPKGC+ELLSRSGISI+GKKAVV+GRSNIVG
Sbjct: 121 SIEKDVDGFHPLNIGKLAMKGREPLFLPCTPKGCLELLSRSGISIKGKKAVVVGRSNIVG 180

Query: 255 LPVSLLLLKADATVTIVHSRSVDPESVIREADIIIAAAGQAQMIKGSWIKPGAAVIDVGT 314
           LPVSLLLLKADATV+IVHSR+ DPE +I EADIIIAAAGQ  MIKGSWIKPGAAVIDVGT
Sbjct: 181 LPVSLLLLKADATVSIVHSRTEDPERIICEADIIIAAAGQPMMIKGSWIKPGAAVIDVGT 240

Query: 315 NAVDDPTKKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMTVAMLLRNTLDGAKRVIEQ 374
           NAVDDP+KKSGYRLVGDVD++EAC VAGWITPVPGGVGPMTVAMLL+NTLDGAKR   Q
Sbjct: 241 NAVDDPSKKSGYRLVGDVDYKEACNVAGWITPVPGGVGPMTVAMLLKNTLDGAKREFMQ 299

[Close]
BLAST of Csa4G001540 vs. TrEMBL
Match: M5XFZ4_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa009263mg PE=3 SV=1)

HSP 1 Score: 520.0 bits (1338), Expect = 2.4e-144
Identity = 258/297 (86.87%), Postives = 280/297 (94.28%), Query Frame = 1 

Query: 75  MASESDHKATIIDGKKIAQTVRSEITEEVNKLSQKYGKIPGLAVVIVGNRKDSLTYVNMK 134
           MAS+SDH A IIDGK IAQT+R+EI EEV  LSQKYGK+PGLAVVIVGNRKDS +YV+MK
Sbjct: 1   MASQSDHMAAIIDGKAIAQTIRNEIAEEVRHLSQKYGKVPGLAVVIVGNRKDSQSYVSMK 60

Query: 135 RKACLEVGIKSFEIDLPEQVSEAELISKVHELNANPEVHGILVQLPLPNHINEEKVLSEI 194
           RKAC EV IKS +IDLPE VS+ +LI+KVHELNANP+VHGILVQLPLP HINEEKVLSEI
Sbjct: 61  RKACAEVAIKSLDIDLPEYVSQDDLIAKVHELNANPDVHGILVQLPLPKHINEEKVLSEI 120

Query: 195 SIEKDVDGFHPLNIGKLAMKGRDPLFLPCTPKGCIELLSRSGISIRGKKAVVMGRSNIVG 254
           SIEKDVDGFHPLNIGKLAMKGR+PLFLPCTPKGC+ELLSRSGISI+GKKAVV+GRSNIVG
Sbjct: 121 SIEKDVDGFHPLNIGKLAMKGREPLFLPCTPKGCLELLSRSGISIKGKKAVVVGRSNIVG 180

Query: 255 LPVSLLLLKADATVTIVHSRSVDPESVIREADIIIAAAGQAQMIKGSWIKPGAAVIDVGT 314
           LPVSLLLLKADATVT+VHS S DPES+IREADIIIAAAGQA MIKGSWIKPGAAVIDVGT
Sbjct: 181 LPVSLLLLKADATVTVVHSHSHDPESIIREADIIIAAAGQAMMIKGSWIKPGAAVIDVGT 240

Query: 315 NAVDDPTKKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMTVAMLLRNTLDGAKRVI 372
           NA+DD ++KSGYRLVGDVDFQEACKVAGW+TPVPGGVGPMTVAMLL+NTLDGAKRVI
Sbjct: 241 NAIDDSSRKSGYRLVGDVDFQEACKVAGWVTPVPGGVGPMTVAMLLKNTLDGAKRVI 297

[Close]
BLAST of Csa4G001540 vs. TrEMBL
Match: A0A061DRG6_THECC (Amino acid dehydrogenase family protein OS=Theobroma cacao GN=TCM_004603 PE=3 SV=1)

HSP 1 Score: 520.0 bits (1338), Expect = 2.4e-144
Identity = 259/299 (86.62%), Postives = 280/299 (93.65%), Query Frame = 1 

Query: 75  MASESDHKATIIDGKKIAQTVRSEITEEVNKLSQKYGKIPGLAVVIVGNRKDSLTYVNMK 134
           MAS SDHKATIIDGK IAQT+RSEI +EV+ L QKYGK+PGLAVVIVG RKDS +YV MK
Sbjct: 1   MASPSDHKATIIDGKAIAQTIRSEIADEVHHLFQKYGKVPGLAVVIVGGRKDSQSYVGMK 60

Query: 135 RKACLEVGIKSFEIDLPEQVSEAELISKVHELNANPEVHGILVQLPLPNHINEEKVLSEI 194
           RKAC EVGIKSF++DLPE+V E+ELISKVHELNAN +VHGILVQLPLP HINEEKVLSEI
Sbjct: 61  RKACAEVGIKSFDVDLPEEVPESELISKVHELNANRDVHGILVQLPLPKHINEEKVLSEI 120

Query: 195 SIEKDVDGFHPLNIGKLAMKGRDPLFLPCTPKGCIELLSRSGISIRGKKAVVMGRSNIVG 254
           S+ KDVDGFHPLNIGKLAMKGR+PLF PCTPKGC+ELL+RSGISI+GK AVV+GRSNIVG
Sbjct: 121 SLAKDVDGFHPLNIGKLAMKGREPLFQPCTPKGCLELLARSGISIKGKNAVVVGRSNIVG 180

Query: 255 LPVSLLLLKADATVTIVHSRSVDPESVIREADIIIAAAGQAQMIKGSWIKPGAAVIDVGT 314
           LPVSLLLLKAD+TVTIVHSR+ DPE ++READIIIAAAGQA MIKGSWIKPGAAVIDVGT
Sbjct: 181 LPVSLLLLKADSTVTIVHSRTPDPERLVREADIIIAAAGQAMMIKGSWIKPGAAVIDVGT 240

Query: 315 NAVDDPTKKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMTVAMLLRNTLDGAKRVIEQ 374
           NAVDDP+KKSGYRLVGDVDF EACKVAGWITPVPGGVGPMTVAMLLRNTLDGAKRVIEQ
Sbjct: 241 NAVDDPSKKSGYRLVGDVDFHEACKVAGWITPVPGGVGPMTVAMLLRNTLDGAKRVIEQ 299

[Close]
BLAST of Csa4G001540 vs. TAIR10
Match: AT3G12290.1 (AT3G12290.1 Amino acid dehydrogenase family protein)

HSP 1 Score: 491.1 bits (1263), Expect = 6.0e-139
Identity = 242/296 (81.76%), Postives = 269/296 (90.88%), Query Frame = 1 

Query: 75  MASESDHKATIIDGKKIAQTVRSEITEEVNKLSQKYGKIPGLAVVIVGNRKDSLTYVNMK 134
           MAS SDH A IIDGK IA T+RSEI EEV  LS+K+GK+PGLAVVIVG+RKDS TYVN K
Sbjct: 1   MASSSDHTAKIIDGKAIAHTIRSEIAEEVRGLSEKHGKVPGLAVVIVGSRKDSQTYVNTK 60

Query: 135 RKACLEVGIKSFEIDLPEQVSEAELISKVHELNANPEVHGILVQLPLPNHINEEKVLSEI 194
           RKAC EVGIKSF++ LPE+VSEA+LISKVHELN+NP+VHGILVQLPLP HINEE +L  I
Sbjct: 61  RKACAEVGIKSFDVGLPEEVSEADLISKVHELNSNPDVHGILVQLPLPKHINEEHILGAI 120

Query: 195 SIEKDVDGFHPLNIGKLAMKGRDPLFLPCTPKGCIELLSRSGISIRGKKAVVMGRSNIVG 254
           SI+KDVDGFHPLNIGKLAMKGR+PLFLPCTPKGC+ELL+RSG+ I+G++AVV+GRSNIVG
Sbjct: 121 SIDKDVDGFHPLNIGKLAMKGREPLFLPCTPKGCLELLARSGVKIKGQRAVVVGRSNIVG 180

Query: 255 LPVSLLLLKADATVTIVHSRSVDPESVIREADIIIAAAGQAQMIKGSWIKPGAAVIDVGT 314
           LPVSLLLLKADATVT VHS + DPE++IREADI+IAA GQA MIKG+WIKPGAAVIDVGT
Sbjct: 181 LPVSLLLLKADATVTTVHSHTKDPEAIIREADIVIAACGQAHMIKGNWIKPGAAVIDVGT 240

Query: 315 NAVDDPTKKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMTVAMLLRNTLDGAKRV 371
           NAV DP+KKSGYRLVGDVDF EA KVAG+ITPVPGGVGPMTVAMLLRNT+DGAKRV
Sbjct: 241 NAVSDPSKKSGYRLVGDVDFAEASKVAGFITPVPGGVGPMTVAMLLRNTVDGAKRV 296

[Close]
BLAST of Csa4G001540 vs. TAIR10
Match: AT4G00620.1 (AT4G00620.1 Amino acid dehydrogenase family protein)

HSP 1 Score: 389.0 bits (998), Expect = 3.2e-108
Identity = 186/294 (63.27%), Postives = 236/294 (80.27%), Query Frame = 1 

Query: 77  SESDHKATIIDGKKIAQTVRSEITEEVNKLSQKYGKIPGLAVVIVGNRKDSLTYVNMKRK 136
           ++S+  A +IDGK +A+ +R EIT EV+++ +  G IPGLAV++VG+RKDS TYV  K+K
Sbjct: 63  TKSEGGAIVIDGKAVAKKIRDEITIEVSRMKESIGVIPGLAVILVGDRKDSATYVRNKKK 122

Query: 137 ACLEVGIKSFEIDLPEQVSEAELISKVHELNANPEVHGILVQLPLPNHINEEKVLSEISI 196
           AC  VGIKSFE+ L E  SE E++  V   N +P VHGILVQLPLP+H++E+ +L+ +SI
Sbjct: 123 ACDSVGIKSFEVRLAEDSSEEEVLKSVSGFNDDPSVHGILVQLPLPSHMDEQNILNAVSI 182

Query: 197 EKDVDGFHPLNIGKLAMKGRDPLFLPCTPKGCIELLSRSGISIRGKKAVVMGRSNIVGLP 256
           EKDVDGFHPLNIG+LAM+GR+PLF+PCTPKGCIELL R  I I+GK+AVV+GRSNIVG+P
Sbjct: 183 EKDVDGFHPLNIGRLAMRGREPLFVPCTPKGCIELLHRYNIEIKGKRAVVIGRSNIVGMP 242

Query: 257 VSLLLLKADATVTIVHSRSVDPESVIREADIIIAAAGQAQMIKGSWIKPGAAVIDVGTNA 316
            +LLL + DATV+I+HSR+ +PE + READIII+A GQ  M++GSWIKPGA +IDVG N 
Sbjct: 243 AALLLQREDATVSIIHSRTKNPEEITREADIIISAVGQPNMVRGSWIKPGAVLIDVGINP 302

Query: 317 VDDPTKKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMTVAMLLRNTLDGAKRV 371
           V+DP+   GYRLVGD+ ++EA KVA  ITPVPGGVGPMT+AMLL NTL  AKR+
Sbjct: 303 VEDPSAARGYRLVGDICYEEASKVASAITPVPGGVGPMTIAMLLSNTLTSAKRI 356

[Close]
BLAST of Csa4G001540 vs. TAIR10
Match: AT2G38660.1 (AT2G38660.1 Amino acid dehydrogenase family protein)

HSP 1 Score: 366.3 bits (939), Expect = 2.2e-101
Identity = 175/299 (58.53%), Postives = 229/299 (76.59%), Query Frame = 1 

Query: 72  PAKMASESDHKATIIDGKKIAQTVRSEITEEVNKLSQKYGKIPGLAVVIVGNRKDSLTYV 131
           P  ++ E++ K  +IDG  IA+ +R++I  EV K+ +  GK+PGLAVV+VG ++DS TYV
Sbjct: 52  PPPVSFETEQKTVVIDGNVIAEEIRTKIISEVGKMKKAVGKVPGLAVVLVGEQRDSQTYV 111

Query: 132 NMKRKACLEVGIKSFEIDLPEQVSEAELISKVHELNANPEVHGILVQLPLPNHINEEKVL 191
             K KAC E GIKS   +LPE  +E ++IS + + N +  +HGILVQLPLP H+NE K+L
Sbjct: 112 RNKIKACEETGIKSVLAELPEDCTEGQIISVLRKFNEDTSIHGILVQLPLPQHLNESKIL 171

Query: 192 SEISIEKDVDGFHPLNIGKLAMKGRDPLFLPCTPKGCIELLSRSGISIRGKKAVVMGRSN 251
           + + +EKDVDGFHPLN+G LAM+GR+PLF+ CTPKGC+ELL R+G+ I GK AVV+GRSN
Sbjct: 172 NMVRLEKDVDGFHPLNVGNLAMRGREPLFVSCTPKGCVELLIRTGVEIAGKNAVVIGRSN 231

Query: 252 IVGLPVSLLLLKADATVTIVHSRSVDPESVIREADIIIAAAGQAQMIKGSWIKPGAAVID 311
           IVGLP+SLLL + DATV+ VH+ + DPE + R+ADI+IAAAG   +++GSW+KPGA VID
Sbjct: 232 IVGLPMSLLLQRHDATVSTVHAFTKDPEHITRKADIVIAAAGIPNLVRGSWLKPGAVVID 291

Query: 312 VGTNAVDDPTKKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMTVAMLLRNTLDGAKRV 371
           VGT  V+D + + GYRLVGDV ++EA  VA  ITPVPGGVGPMT+ MLL NTL+ AKR+
Sbjct: 292 VGTTPVEDSSCEFGYRLVGDVCYEEALGVASAITPVPGGVGPMTITMLLCNTLEAAKRI 350

[Close]
BLAST of Csa4G001540 vs. TAIR10
Match: AT4G00600.1 (AT4G00600.1 Amino acid dehydrogenase family protein)

HSP 1 Score: 292.7 bits (748), Expect = 3.1e-79
Identity = 137/219 (62.56%), Postives = 175/219 (79.91%), Query Frame = 1 

Query: 152 EQVSEAELISKVHELNANPEVHGILVQLPLPNHINEEKVLSEISIEKDVDGFHPLNIGKL 211
           E  SE E++  V   N +P VHG+LVQLPLP+H++E+ +L+ +SIEKDVDGFHPLNIG+L
Sbjct: 88  EDSSEEEVLKYVSGFNDDPSVHGVLVQLPLPSHMDEQNILNAVSIEKDVDGFHPLNIGRL 147

Query: 212 AMKGRDPLFLPCTPKGCIELLSRSGISIRGKKAVVMGRSNIVGLPVSLLLLKADATVTIV 271
           AM+GR+PLF+PCTPKGCIELL R  I  +GK+AVV+GRSNIVG+P +LLL K DATV+I+
Sbjct: 148 AMRGREPLFVPCTPKGCIELLHRYNIEFKGKRAVVIGRSNIVGMPAALLLQKEDATVSII 207

Query: 272 HSRSVDPESVIREADIIIAAAGQAQMIKGSWIKPGAAVIDVGTNAVDDPTKKSGYRLVGD 331
           HSR+++PE + R+ADI+I+A G+  M++GSWIKPGA +IDVG   V+DP+   G RLVGD
Sbjct: 208 HSRTMNPEELTRQADILISAVGKPNMVRGSWIKPGAVLIDVGIKPVEDPSAAGGERLVGD 267

Query: 332 VDFQEACKVAGWITPVPGGVGPMTVAMLLRNTLDGAKRV 371
           + + EA K+A  ITPVPG VGPMT+AMLL NTL  AKR+
Sbjct: 268 ICYVEASKIASAITPVPGDVGPMTIAMLLSNTLTSAKRI 306

[Close]
BLAST of Csa4G001540 vs. NCBI nr
Match: gi|700197629|gb|KGN52787.1| (hypothetical protein Csa_4G001540 [Cucumis sativus])

HSP 1 Score: 735.7 bits (1898), Expect = 4.0e-209
Identity = 373/373 (100.00%), Postives = 373/373 (100.00%), Query Frame = 1 

Query: 1   MGHTKLCTDVPISRQTQSDCPNPSPSTYSHACINSLIIHNQSFSISASLLHAIATGSCKF 60
           MGHTKLCTDVPISRQTQSDCPNPSPSTYSHACINSLIIHNQSFSISASLLHAIATGSCKF
Sbjct: 1   MGHTKLCTDVPISRQTQSDCPNPSPSTYSHACINSLIIHNQSFSISASLLHAIATGSCKF 60

Query: 61  NSEVVTIFFFSPAKMASESDHKATIIDGKKIAQTVRSEITEEVNKLSQKYGKIPGLAVVI 120
           NSEVVTIFFFSPAKMASESDHKATIIDGKKIAQTVRSEITEEVNKLSQKYGKIPGLAVVI
Sbjct: 61  NSEVVTIFFFSPAKMASESDHKATIIDGKKIAQTVRSEITEEVNKLSQKYGKIPGLAVVI 120

Query: 121 VGNRKDSLTYVNMKRKACLEVGIKSFEIDLPEQVSEAELISKVHELNANPEVHGILVQLP 180
           VGNRKDSLTYVNMKRKACLEVGIKSFEIDLPEQVSEAELISKVHELNANPEVHGILVQLP
Sbjct: 121 VGNRKDSLTYVNMKRKACLEVGIKSFEIDLPEQVSEAELISKVHELNANPEVHGILVQLP 180

Query: 181 LPNHINEEKVLSEISIEKDVDGFHPLNIGKLAMKGRDPLFLPCTPKGCIELLSRSGISIR 240
           LPNHINEEKVLSEISIEKDVDGFHPLNIGKLAMKGRDPLFLPCTPKGCIELLSRSGISIR
Sbjct: 181 LPNHINEEKVLSEISIEKDVDGFHPLNIGKLAMKGRDPLFLPCTPKGCIELLSRSGISIR 240

Query: 241 GKKAVVMGRSNIVGLPVSLLLLKADATVTIVHSRSVDPESVIREADIIIAAAGQAQMIKG 300
           GKKAVVMGRSNIVGLPVSLLLLKADATVTIVHSRSVDPESVIREADIIIAAAGQAQMIKG
Sbjct: 241 GKKAVVMGRSNIVGLPVSLLLLKADATVTIVHSRSVDPESVIREADIIIAAAGQAQMIKG 300

Query: 301 SWIKPGAAVIDVGTNAVDDPTKKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMTVAMLL 360
           SWIKPGAAVIDVGTNAVDDPTKKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMTVAMLL
Sbjct: 301 SWIKPGAAVIDVGTNAVDDPTKKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMTVAMLL 360

Query: 361 RNTLDGAKRVIEQ 374
           RNTLDGAKRVIEQ
Sbjct: 361 RNTLDGAKRVIEQ 373

[Close]
BLAST of Csa4G001540 vs. NCBI nr
Match: gi|449469104|ref|XP_004152261.1| (PREDICTED: bifunctional protein FolD 2 isoform X1 [Cucumis sativus])

HSP 1 Score: 585.1 bits (1507), Expect = 8.7e-164
Identity = 299/299 (100.00%), Postives = 299/299 (100.00%), Query Frame = 1 

Query: 75  MASESDHKATIIDGKKIAQTVRSEITEEVNKLSQKYGKIPGLAVVIVGNRKDSLTYVNMK 134
           MASESDHKATIIDGKKIAQTVRSEITEEVNKLSQKYGKIPGLAVVIVGNRKDSLTYVNMK
Sbjct: 1   MASESDHKATIIDGKKIAQTVRSEITEEVNKLSQKYGKIPGLAVVIVGNRKDSLTYVNMK 60

Query: 135 RKACLEVGIKSFEIDLPEQVSEAELISKVHELNANPEVHGILVQLPLPNHINEEKVLSEI 194
           RKACLEVGIKSFEIDLPEQVSEAELISKVHELNANPEVHGILVQLPLPNHINEEKVLSEI
Sbjct: 61  RKACLEVGIKSFEIDLPEQVSEAELISKVHELNANPEVHGILVQLPLPNHINEEKVLSEI 120

Query: 195 SIEKDVDGFHPLNIGKLAMKGRDPLFLPCTPKGCIELLSRSGISIRGKKAVVMGRSNIVG 254
           SIEKDVDGFHPLNIGKLAMKGRDPLFLPCTPKGCIELLSRSGISIRGKKAVVMGRSNIVG
Sbjct: 121 SIEKDVDGFHPLNIGKLAMKGRDPLFLPCTPKGCIELLSRSGISIRGKKAVVMGRSNIVG 180

Query: 255 LPVSLLLLKADATVTIVHSRSVDPESVIREADIIIAAAGQAQMIKGSWIKPGAAVIDVGT 314
           LPVSLLLLKADATVTIVHSRSVDPESVIREADIIIAAAGQAQMIKGSWIKPGAAVIDVGT
Sbjct: 181 LPVSLLLLKADATVTIVHSRSVDPESVIREADIIIAAAGQAQMIKGSWIKPGAAVIDVGT 240

Query: 315 NAVDDPTKKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMTVAMLLRNTLDGAKRVIEQ 374
           NAVDDPTKKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMTVAMLLRNTLDGAKRVIEQ
Sbjct: 241 NAVDDPTKKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMTVAMLLRNTLDGAKRVIEQ 299

[Close]
BLAST of Csa4G001540 vs. NCBI nr
Match: gi|659108749|ref|XP_008454369.1| (PREDICTED: bifunctional protein FolD 2 isoform X1 [Cucumis melo])

HSP 1 Score: 578.9 bits (1491), Expect = 6.2e-162
Identity = 293/299 (97.99%), Postives = 297/299 (99.33%), Query Frame = 1 

Query: 75  MASESDHKATIIDGKKIAQTVRSEITEEVNKLSQKYGKIPGLAVVIVGNRKDSLTYVNMK 134
           MASESDHKATIIDGKKIAQTVRSE+ EEVNKLS+KYGK+PGLAVVIVGNRKDSLTYVNMK
Sbjct: 1   MASESDHKATIIDGKKIAQTVRSEVAEEVNKLSEKYGKVPGLAVVIVGNRKDSLTYVNMK 60

Query: 135 RKACLEVGIKSFEIDLPEQVSEAELISKVHELNANPEVHGILVQLPLPNHINEEKVLSEI 194
           RKACLEVGIKSFEIDLPEQVSEAELISKVHELNANPEVHGILVQLPLPNHINEEKVLSEI
Sbjct: 61  RKACLEVGIKSFEIDLPEQVSEAELISKVHELNANPEVHGILVQLPLPNHINEEKVLSEI 120

Query: 195 SIEKDVDGFHPLNIGKLAMKGRDPLFLPCTPKGCIELLSRSGISIRGKKAVVMGRSNIVG 254
           SIEKDVDGFHPLNIGKLAMKGRDPLFLPCTPKGC+ELLSRSGISIRGKKAVVMGRSNIVG
Sbjct: 121 SIEKDVDGFHPLNIGKLAMKGRDPLFLPCTPKGCLELLSRSGISIRGKKAVVMGRSNIVG 180

Query: 255 LPVSLLLLKADATVTIVHSRSVDPESVIREADIIIAAAGQAQMIKGSWIKPGAAVIDVGT 314
           LPVSLLLLKADATVTIVHSRSVDPESVIREADIIIAAAGQAQMIKGSWIKPGAAVIDVGT
Sbjct: 181 LPVSLLLLKADATVTIVHSRSVDPESVIREADIIIAAAGQAQMIKGSWIKPGAAVIDVGT 240

Query: 315 NAVDDPTKKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMTVAMLLRNTLDGAKRVIEQ 374
           NAVDDPTKKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMTVAMLLRNTLDGAKR IEQ
Sbjct: 241 NAVDDPTKKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMTVAMLLRNTLDGAKRAIEQ 299

[Close]
BLAST of Csa4G001540 vs. NCBI nr
Match: gi|645258763|ref|XP_008235037.1| (PREDICTED: bifunctional protein FolD 2-like [Prunus mume])

HSP 1 Score: 524.6 bits (1350), Expect = 1.4e-145
Identity = 260/299 (86.96%), Postives = 282/299 (94.31%), Query Frame = 1 

Query: 75  MASESDHKATIIDGKKIAQTVRSEITEEVNKLSQKYGKIPGLAVVIVGNRKDSLTYVNMK 134
           MAS+SDHKA IIDGK IAQT+R+EI EEV  LSQKYGK+PGLAVVIVGNRKDS +YV+MK
Sbjct: 1   MASQSDHKAAIIDGKAIAQTIRNEIAEEVRHLSQKYGKVPGLAVVIVGNRKDSQSYVSMK 60

Query: 135 RKACLEVGIKSFEIDLPEQVSEAELISKVHELNANPEVHGILVQLPLPNHINEEKVLSEI 194
           RKAC EVGI S +IDLPE VS+ +LI+KVHELNANP+VHGILVQLPLP HINEEKVLSEI
Sbjct: 61  RKACAEVGILSLDIDLPEDVSQVDLIAKVHELNANPDVHGILVQLPLPKHINEEKVLSEI 120

Query: 195 SIEKDVDGFHPLNIGKLAMKGRDPLFLPCTPKGCIELLSRSGISIRGKKAVVMGRSNIVG 254
           SIEKDVDGFHPLNIGKLAMKGR+PLFLPCTPKGC+ELLSRSGISI+GKKAVV+GRSNIVG
Sbjct: 121 SIEKDVDGFHPLNIGKLAMKGREPLFLPCTPKGCLELLSRSGISIKGKKAVVVGRSNIVG 180

Query: 255 LPVSLLLLKADATVTIVHSRSVDPESVIREADIIIAAAGQAQMIKGSWIKPGAAVIDVGT 314
           LPVSLLLLKADATVT+VHS S DPES+IREADIIIAAAGQA MIKGSWIKPGAAVIDVGT
Sbjct: 181 LPVSLLLLKADATVTVVHSHSHDPESIIREADIIIAAAGQAMMIKGSWIKPGAAVIDVGT 240

Query: 315 NAVDDPTKKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMTVAMLLRNTLDGAKRVIEQ 374
           NA+DD ++KSGYRLVGDVDFQEACKVAGW+TPVPGGVGPMTVAMLL+NTLDGAKRVI Q
Sbjct: 241 NAIDDSSRKSGYRLVGDVDFQEACKVAGWVTPVPGGVGPMTVAMLLKNTLDGAKRVIAQ 299

[Close]
BLAST of Csa4G001540 vs. NCBI nr
Match: gi|595793509|ref|XP_007200503.1| (hypothetical protein PRUPE_ppa009269mg [Prunus persica])

HSP 1 Score: 523.5 bits (1347), Expect = 3.1e-145
Identity = 259/299 (86.62%), Postives = 281/299 (93.98%), Query Frame = 1 

Query: 75  MASESDHKATIIDGKKIAQTVRSEITEEVNKLSQKYGKIPGLAVVIVGNRKDSLTYVNMK 134
           MAS+SDHKA IIDGK IAQT+R+EI EEV  LSQKYGK+PGLAVVIVGNRKDS +YV+MK
Sbjct: 1   MASQSDHKAAIIDGKAIAQTIRNEIAEEVRHLSQKYGKVPGLAVVIVGNRKDSQSYVSMK 60

Query: 135 RKACLEVGIKSFEIDLPEQVSEAELISKVHELNANPEVHGILVQLPLPNHINEEKVLSEI 194
           RKAC EVGI S +IDLPE VS+ +LI+KVHELNANP+VHGILVQLPLP HINEEKVLSEI
Sbjct: 61  RKACAEVGILSLDIDLPEDVSQVDLIAKVHELNANPDVHGILVQLPLPKHINEEKVLSEI 120

Query: 195 SIEKDVDGFHPLNIGKLAMKGRDPLFLPCTPKGCIELLSRSGISIRGKKAVVMGRSNIVG 254
           SIEKDVDGFHPLNIGKLAMKGR+PLFLPCTPKGC+ELLSRSGISI+GKKAVV+GRSNIVG
Sbjct: 121 SIEKDVDGFHPLNIGKLAMKGREPLFLPCTPKGCLELLSRSGISIKGKKAVVVGRSNIVG 180

Query: 255 LPVSLLLLKADATVTIVHSRSVDPESVIREADIIIAAAGQAQMIKGSWIKPGAAVIDVGT 314
           LPVSLLLLKADATVT+VHS S DPES+IREADI+IAAAGQA MIKGSWIKPGAAVIDVGT
Sbjct: 181 LPVSLLLLKADATVTVVHSHSHDPESIIREADIVIAAAGQAMMIKGSWIKPGAAVIDVGT 240

Query: 315 NAVDDPTKKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMTVAMLLRNTLDGAKRVIEQ 374
           NA+DD ++KSGYRLVGDVDFQEACKVAGW+TPVPGGVGPMTVAMLL NTLDGAKRVI Q
Sbjct: 241 NAIDDSSRKSGYRLVGDVDFQEACKVAGWVTPVPGGVGPMTVAMLLNNTLDGAKRVIAQ 299

The following BLAST results are available for this feature:
BLAST of Csa4G001540 vs. Swiss-Prot
Match NameE-valueIdentityDescription
FOLD2_ARATH1.1e-13781.76Bifunctional protein FolD 2 OS=Arabidopsis thaliana GN=FOLD2 PE=2 SV=1[more]
FOLD4_ARATH5.7e-10763.27Bifunctional protein FolD 4, chloroplastic OS=Arabidopsis thaliana GN=FOLD4 PE=1... [more]
FOLD1_ARATH4.0e-10058.53Bifunctional protein FolD 1, mitochondrial OS=Arabidopsis thaliana GN=FOLD1 PE=2... [more]
FOLD_MOOTA5.4e-8155.75Bifunctional protein FolD OS=Moorella thermoacetica (strain ATCC 39073) GN=folD ... [more]
FOLD_LISMC1.2e-8055.94Bifunctional protein FolD OS=Listeria monocytogenes serotype 4b (strain CLIP8045... [more]
BLAST of Csa4G001540 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KWH1_CUCSA2.8e-209100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_4G001540 PE=3 SV=1[more]
M5VKQ0_PRUPE2.2e-14586.62Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa009269mg PE=3 SV=1[more]
B9T0V4_RICCO2.4e-14486.96Methylenetetrahydrofolate dehydrogenase, putative OS=Ricinus communis GN=RCOM_14... [more]
M5XFZ4_PRUPE2.4e-14486.87Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa009263mg PE=3 SV=1[more]
A0A061DRG6_THECC2.4e-14486.62Amino acid dehydrogenase family protein OS=Theobroma cacao GN=TCM_004603 PE=3 SV... [more]
BLAST of Csa4G001540 vs. TAIR10
Match NameE-valueIdentityDescription
AT3G12290.16.0e-13981.76 Amino acid dehydrogenase family protein[more]
AT4G00620.13.2e-10863.27 Amino acid dehydrogenase family protein[more]
AT2G38660.12.2e-10158.53 Amino acid dehydrogenase family protein[more]
AT4G00600.13.1e-7962.56 Amino acid dehydrogenase family protein[more]
BLAST of Csa4G001540 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|700197629|gb|KGN52787.1|4.0e-209100.00hypothetical protein Csa_4G001540 [Cucumis sativus][more]
gi|449469104|ref|XP_004152261.1|8.7e-164100.00PREDICTED: bifunctional protein FolD 2 isoform X1 [Cucumis sativus][more]
gi|659108749|ref|XP_008454369.1|6.2e-16297.99PREDICTED: bifunctional protein FolD 2 isoform X1 [Cucumis melo][more]
gi|645258763|ref|XP_008235037.1|1.4e-14586.96PREDICTED: bifunctional protein FolD 2-like [Prunus mume][more]
gi|595793509|ref|XP_007200503.1|3.1e-14586.62hypothetical protein PRUPE_ppa009269mg [Prunus persica][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000672THF_DH/CycHdrlase
IPR016040NAD(P)-bd_dom
IPR020630THF_DH/CycHdrlase_cat_dom
IPR020631THF_DH/CycHdrlase_NAD-bd_dom
IPR020867THF_DH/CycHdrlase_CS
Vocabulary: Molecular Function
TermDefinition
GO:0003824catalytic activity
GO:0004488methylenetetrahydrofolate dehydrogenase (NADP+) activity
Vocabulary: Biological Process
TermDefinition
GO:0009396folic acid-containing compound biosynthetic process
GO:0055114oxidation-reduction process
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009396 folic acid-containing compound biosynthetic process
biological_process GO:0046487 glyoxylate metabolic process
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0030244 cellulose biosynthetic process
biological_process GO:0019344 cysteine biosynthetic process
biological_process GO:0048193 Golgi vesicle transport
cellular_component GO:0009507 chloroplast
cellular_component GO:0005829 cytosol
cellular_component GO:0005575 cellular_component
molecular_function GO:0004477 methenyltetrahydrofolate cyclohydrolase activity
molecular_function GO:0004488 methylenetetrahydrofolate dehydrogenase (NADP+) activity
molecular_function GO:0003824 catalytic activity
molecular_function GO:0016787 hydrolase activity
Unigene
This gene is associated with the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
CU086076cucumber EST collection version 3.0transcribed_cluster
CU103984cucumber EST collection version 3.0transcribed_cluster
CU106370cucumber EST collection version 3.0transcribed_cluster
CU113469cucumber EST collection version 3.0transcribed_cluster
CU114851cucumber EST collection version 3.0transcribed_cluster
CU138981cucumber EST collection version 3.0transcribed_cluster
CU152170cucumber EST collection version 3.0transcribed_cluster
CU160048cucumber EST collection version 3.0transcribed_cluster
CU160640cucumber EST collection version 3.0transcribed_cluster
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csa4G001540.1Csa4G001540.1mRNA


The following transcribed_cluster feature(s) are associated with this gene:

Feature NameUnique NameType
CU106370CU106370transcribed_cluster
CU152170CU152170transcribed_cluster
CU114851CU114851transcribed_cluster
CU113469CU113469transcribed_cluster
CU160048CU160048transcribed_cluster
CU160640CU160640transcribed_cluster
CU086076CU086076transcribed_cluster
CU138981CU138981transcribed_cluster
CU103984CU103984transcribed_cluster


InterPro
Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000672Tetrahydrofolate dehydrogenase/cyclohydrolasePRINTSPR00085THFDHDRGNASEcoord: 114..136
score: 1.4E-81coord: 190..211
score: 1.4E-81coord: 155..182
score: 1.4E-81coord: 327..343
score: 1.4E-81coord: 344..362
score: 1.4E-81coord: 237..257
score: 1.4E-81coord: 286..315
score: 1.4
IPR000672Tetrahydrofolate dehydrogenase/cyclohydrolaseHAMAPMF_01576THF_DHG_CYHcoord: 83..371
score: 37
IPR016040NAD(P)-binding domainGENE3DG3DSA:3.40.50.720coord: 222..352
score: 1.5
IPR016040NAD(P)-binding domainunknownSSF51735NAD(P)-binding Rossmann-fold domainscoord: 203..366
score: 2.69
IPR020630Tetrahydrofolate dehydrogenase/cyclohydrolase, catalytic domainPFAMPF00763THF_DHG_CYHcoord: 85..201
score: 2.2
IPR020631Tetrahydrofolate dehydrogenase/cyclohydrolase, NAD(P)-binding domainPFAMPF02882THF_DHG_CYH_Ccoord: 204..369
score: 5.0
IPR020867Tetrahydrofolate dehydrogenase/cyclohydrolase, conserved sitePROSITEPS00766THF_DHG_CYH_1coord: 156..181
scor
IPR020867Tetrahydrofolate dehydrogenase/cyclohydrolase, conserved sitePROSITEPS00767THF_DHG_CYH_2coord: 348..356
scor
NoneNo IPR availableGENE3DG3DSA:3.40.192.10coord: 87..221
score: 8.4
NoneNo IPR availablePANTHERPTHR10025TETRAHYDROFOLATE DEHYDROGENASE/CYCLOHYDROLASE FAMILY MEMBERcoord: 73..373
score: 2.2E
NoneNo IPR availablePANTHERPTHR10025:SF33BIFUNCTIONAL PROTEIN FOLD 2coord: 73..373
score: 2.2E
NoneNo IPR availableunknownSSF53223Aminoacid dehydrogenase-like, N-terminal domaincoord: 83..202
score: 4.6