Csa3G188350 (gene) Cucumber (Chinese Long) v2 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Csa3G188350
Type	gene
Organism	Cucumis. sativus (Cucumber (Chinese Long) v2)
Description	Aspartic proteinase nepenthesin-1; contains IPR001461 (Peptidase A1), IPR021109 (Aspartic peptidase)
Location	Chr3 : 13280182 .. 13281543 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATCAGCAACCCCCTCCCCACAATCAACAATGCTTCTGATTCTCTTCTCTCTCTCCTTATTCACTCTCTCCTTCTCTCAATCCAATTCCCTCTCTCTCCCCTTCCCTCTTTCTCTCTCTGAAAAACCCTCCAATACTATCCCATCATACTCTTCCCAGCTTTACGCCAAGAGGCCATCCTCCTACGGCTCCTTCAAGCTTCCTTTCAAATACTCCTCCACCGCCCTCGTCGTCTCTCTACCGATCGGAACGCCGCCACAGCCCACTGACTTGGTTCTAGACACCGGCAGCCAACTCTCTTGGATTCAATGTCATGACAAAAAAGTTAAGAAAAGATTGCCCCCCTTGCCCAAGCCTAAAACCGCCTCCTTTGATCCTTCTCTCTCCTCTTCTTTTTCTCTCCTCCCTTGTAATCACCCCATCTGCAAACCCCGAATTCCAGATTTTACCCTTCCCACTTCTTGTGACCAAAATCGCCTCTGCCACTACTCCTACTTCTACGCTGACGGTACCTTGGCTGAGGGAAATCTCGTCAGAGAAAAATTTACCTTCTCTAAATCCCTTTCTACCCCTCCCGTCATCCTCGGTTGCGCTCAAGCCTCCACCGAAAACAGGGGTATTTTGGGAATGAACCGTGGACGTTTGTCCTTTATCTCCCAAGCTAAAATCTCCAAATTCTCCTATTGCGTTCCGAGTCGAACAGGGTCTAATCCCACCGGGCTATTCTACCTGGGAGATAACCCCAATTCTTCCAAATTCAAATACGTCACCATGTTGACTTTTCCTGAAAGTCAAAGCTCTCCGAATCTCGACCCACTGGCTTACACTCTCCCTATGAAGGCAATAAAAATAGCCGGAAAACGGTTAAACATCCCCCCAGCCGCTTTCAAACCGGATGCGGGTGGGTCGGGTCAAACCATGATTGACTCCGGTTCGGACCTGACTTATTTAGTGGATGAAGCGTATGAGAAGGTTAAAGAGGAGGTAGTGAGATTAGTGGGTGCGATGATGAAGAAAGGCTACGTATACGCCGACGTAGCCGACATGTGTTTCGACGCCGGTGTCACGGCGGAGGTGGGCCGCAGGATTGGCGGCATCTCGTTTGAGTTTGATAACGGAGTGGAGATTTTCGTGGGGAGAGGAGAAGGGGTTTTGACGGAAGTGGAAAAAGGAGTGAAGTGTGTGGGGATCGGACGGTCAGAAAGGCTTGGGATTGGGAGTAATATAATCGGGACTGTTCATCAACAGAATATGTGGGTGGAGTATGATTTGGCCAATAAGAGAGTAGGGTTTGGTGGAGCTGAGTGTAGCAGATTGAAGTGATGATGGGCGGTAAAGATTTATACACGTGTGTGGTTTTTGGA

mRNA sequence

ATGCTTCTGATTCTCTTCTCTCTCTCCTTATTCACTCTCTCCTTCTCTCAATCCAATTCCCTCTCTCTCCCCTTCCCTCTTTCTCTCTCTGAAAAACCCTCCAATACTATCCCATCATACTCTTCCCAGCTTTACGCCAAGAGGCCATCCTCCTACGGCTCCTTCAAGCTTCCTTTCAAATACTCCTCCACCGCCCTCGTCGTCTCTCTACCGATCGGAACGCCGCCACAGCCCACTGACTTGGTTCTAGACACCGGCAGCCAACTCTCTTGGATTCAATGTCATGACAAAAAAGTTAAGAAAAGATTGCCCCCCTTGCCCAAGCCTAAAACCGCCTCCTTTGATCCTTCTCTCTCCTCTTCTTTTTCTCTCCTCCCTTGTAATCACCCCATCTGCAAACCCCGAATTCCAGATTTTACCCTTCCCACTTCTTGTGACCAAAATCGCCTCTGCCACTACTCCTACTTCTACGCTGACGGTACCTTGGCTGAGGGAAATCTCGTCAGAGAAAAATTTACCTTCTCTAAATCCCTTTCTACCCCTCCCGTCATCCTCGGTTGCGCTCAAGCCTCCACCGAAAACAGGGCCGCTTTCAAACCGGATGCGGGTGGGTCGGGTCAAACCATGATTGACTCCGGTTCGGACCTGACTTATTTAGTGGATGAAGCGTATGAGAAGGTTAAAGAGGAGGTAGTGAGATTAGTGGGTGCGATGATGAAGAAAGGCTACGTATACGCCGACGTAGCCGACATGTGTTTCGACGCCGGTGTCACGGCGGAGGTGGGCCGCAGGATTGGCGGCATCTCGTTTGAGTTTGATAACGGAGTGGAGATTTTCGTGGGGAGAGGAGAAGGGGTTTTGACGGAAGTGGAAAAAGGAGTGAAGTGTGTGGGGATCGGACGGTCAGAAAGGCTTGGGATTGGGAGTAATATAATCGGGACTGTTCATCAACAGAATATGTGGGTGGAGTATGATTTGGCCAATAAGAGAGTAGGGTTTGGTGGAGCTGAGTGTAGCAGATTGAAGTGA

Coding sequence (CDS)

ATGCTTCTGATTCTCTTCTCTCTCTCCTTATTCACTCTCTCCTTCTCTCAATCCAATTCCCTCTCTCTCCCCTTCCCTCTTTCTCTCTCTGAAAAACCCTCCAATACTATCCCATCATACTCTTCCCAGCTTTACGCCAAGAGGCCATCCTCCTACGGCTCCTTCAAGCTTCCTTTCAAATACTCCTCCACCGCCCTCGTCGTCTCTCTACCGATCGGAACGCCGCCACAGCCCACTGACTTGGTTCTAGACACCGGCAGCCAACTCTCTTGGATTCAATGTCATGACAAAAAAGTTAAGAAAAGATTGCCCCCCTTGCCCAAGCCTAAAACCGCCTCCTTTGATCCTTCTCTCTCCTCTTCTTTTTCTCTCCTCCCTTGTAATCACCCCATCTGCAAACCCCGAATTCCAGATTTTACCCTTCCCACTTCTTGTGACCAAAATCGCCTCTGCCACTACTCCTACTTCTACGCTGACGGTACCTTGGCTGAGGGAAATCTCGTCAGAGAAAAATTTACCTTCTCTAAATCCCTTTCTACCCCTCCCGTCATCCTCGGTTGCGCTCAAGCCTCCACCGAAAACAGGGCCGCTTTCAAACCGGATGCGGGTGGGTCGGGTCAAACCATGATTGACTCCGGTTCGGACCTGACTTATTTAGTGGATGAAGCGTATGAGAAGGTTAAAGAGGAGGTAGTGAGATTAGTGGGTGCGATGATGAAGAAAGGCTACGTATACGCCGACGTAGCCGACATGTGTTTCGACGCCGGTGTCACGGCGGAGGTGGGCCGCAGGATTGGCGGCATCTCGTTTGAGTTTGATAACGGAGTGGAGATTTTCGTGGGGAGAGGAGAAGGGGTTTTGACGGAAGTGGAAAAAGGAGTGAAGTGTGTGGGGATCGGACGGTCAGAAAGGCTTGGGATTGGGAGTAATATAATCGGGACTGTTCATCAACAGAATATGTGGGTGGAGTATGATTTGGCCAATAAGAGAGTAGGGTTTGGTGGAGCTGAGTGTAGCAGATTGAAGTGA

Protein sequence

MLLILFSLSLFTLSFSQSNSLSLPFPLSLSEKPSNTIPSYSSQLYAKRPSSYGSFKLPFKYSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKVKKRLPPLPKPKTASFDPSLSSSFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSKSLSTPPVILGCAQASTENRAAFKPDAGGSGQTMIDSGSDLTYLVDEAYEKVKEEVVRLVGAMMKKGYVYADVADMCFDAGVTAEVGRRIGGISFEFDNGVEIFVGRGEGVLTEVEKGVKCVGIGRSERLGIGSNIIGTVHQQNMWVEYDLANKRVGFGGAECSRLK*

Homology

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BLAST of Csa3G188350 vs. Swiss-Prot
Match: PCS1L_ARATH (Aspartic proteinase PCS1 OS=Arabidopsis thaliana GN=PCS1 PE=2 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 1.7e-25
Identity = 71/182 (39.01%), Postives = 100/182 (54.95%), Query Frame = 1


Query: 6   FSLSLFTLSFSQSNSLSLPFPLSLSEKPSNTIPSYSSQLYAKRPSSYGSFKLPFKYSSTA 65
           FS S F+   S S+S +L  PL     P++            RP+     KL F ++ T 
Sbjct: 32  FSFSSFS---SSSSSQTLVLPLKTRITPTD-----------HRPTD----KLHFHHNVT- 91

Query: 66  LVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKVKKRLPPLPKPKTASFDPSLSSSFSLL 125
           L V+L +GTPPQ   +V+DTGS+LSW++C+           P P   +FDP+ SSS+S +
Sbjct: 92  LTVTLTVGTPPQNISMVIDTGSELSWLRCNRSSN-------PNPVN-NFDPTRSSSYSPI 151

Query: 126 PCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSKSLSTPPVIL 185
           PC+ P C+ R  DF +P SCD ++LCH +  YAD + +EGNL  E F F  S +   +I 
Sbjct: 152 PCSSPTCRTRTRDFLIPASCDSDKLCHATLSYADASSSEGNLAAEIFHFGNSTNDSNLIF 186

Query: 186 GC 188
           GC
Sbjct: 212 GC 186

HSP 2 Score: 78.6 bits (192), Expect = 1.5e-13
Identity = 46/157 (29.30%), Postives = 76/157 (48.41%), Query Frame = 1


Query: 195 RAAFKPDAGGSGQTMIDSGSDLTYLVDEAYEKVKEEVVRLVGAMMK----KGYVYADVAD 254
           ++   PD  G+GQTM+DSG+  T+L+   Y  ++   +     ++       +V+    D
Sbjct: 288 KSVLVPDHTGAGQTMVDSGTQFTFLLGPVYTALRSHFLNRTNGILTVYEDPDFVFQGTMD 347

Query: 255 MCFDAG---VTAEVGRRIGGISFEFDNGVEIFVGRGEGVLTEV------EKGVKCVGIGR 314
           +C+      + + +  R+  +S  F+ G EI V  G+ +L  V         V C   G 
Sbjct: 348 LCYRISPVRIRSGILHRLPTVSLVFE-GAEIAVS-GQPLLYRVPHLTVGNDSVYCFTFGN 407

Query: 315 SERLGIGSNIIGTVHQQNMWVEYDLANKRVGFGGAEC 339
           S+ +G+ + +IG  HQQNMW+E+DL   R+G    EC
Sbjct: 408 SDLMGMEAYVIGHHHQQNMWIEFDLQRSRIGLAPVEC 442

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BLAST of Csa3G188350 vs. Swiss-Prot
Match: NEP2_NEPGR (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis GN=nep2 PE=1 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 7.5e-13
Identity = 44/123 (35.77%), Postives = 57/123 (46.34%), Query Frame = 1


Query: 67  VVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKVKKRLPPLPKPKTASFDPSLSSSFSLLP 126
           ++++ IGTP      ++DTGS L W QC         P      T  F+P  SSSFS LP
Sbjct: 97  LMNVAIGTPDSSFSAIMDTGSDLIWTQCEPCTQCFSQP------TPIFNPQDSSSFSTLP 156

Query: 127 CNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSKSLSTPPVILG 186
           C    C+       LP+    N  C Y+Y Y DG+  +G +  E FTF  S S P +  G
Sbjct: 157 CESQYCQD------LPSETCNNNECQYTYGYGDGSTTQGYMATETFTFETS-SVPNIAFG 206

Query: 187 CAQ 190
           C +
Sbjct: 217 CGE 206

HSP 2 Score: 59.3 bits (142), Expect = 9.5e-08
Identity = 47/146 (32.19%), Postives = 68/146 (46.58%), Query Frame = 1


Query: 196 AAFKPDAGGSGQTMIDSGSDLTYLVDEAYEKVKEEVVRLVGAMMKKGYVYADVADMCFDA 255
           + F+    G+G  +IDSG+ LTYL  +AY  V +     +   +      +     CF  
Sbjct: 300 STFQLQDDGTGGMIIDSGTTLTYLPQDAYNAVAQAFTDQIN--LPTVDESSSGLSTCFQQ 359

Query: 256 ---GVTAEVGRRIGGISFEFDNGVEIFVGRGEGVLTEVEKGVKCVGIGRSERLGIGSNII 315
              G T +V      IS +FD GV + +G  + +L    +GV C+ +G S +LGI  +I 
Sbjct: 360 PSDGSTVQVPE----ISMQFDGGV-LNLGE-QNILISPAEGVICLAMGSSSQLGI--SIF 419

Query: 316 GTVHQQNMWVEYDLANKRVGFGGAEC 339
           G + QQ   V YDL N  V F   +C
Sbjct: 420 GNIQQQETQVLYDLQNLAVSFVPTQC 435

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BLAST of Csa3G188350 vs. Swiss-Prot
Match: AED1_ARATH (Aspartyl protease AED1 OS=Arabidopsis thaliana GN=AED1 PE=2 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 9.8e-13
Identity = 60/191 (31.41%), Postives = 84/191 (43.98%), Query Frame = 1


Query: 36  TIPSYSSQLYAKRPSSYGSFKLPFKYSSTA----LVVSLPIGTPPQPTDLVLDTGSQLSW 95
           +I S  S+  A   S   S +LP K   T      +V++ IGTP     LV DTGS L+W
Sbjct: 98  SIYSKLSKNSANEVSEAKSTELPAKSGITLGSGNYIVTIGIGTPKHDLSLVFDTGSDLTW 157

Query: 96  IQCHDKKVKKRLPPLPKPKTASFDPSLSSSFSLLPCNHPICKPRIPDFTLPTSCDQNRLC 155
            QC     +  L      K   F+PS SS++  + C+ P+C+          SC  +  C
Sbjct: 158 TQC-----EPCLGSCYSQKEPKFNPSSSSTYQNVSCSSPMCED-------AESCSASN-C 217

Query: 156 HYSYFYADGTLAEGNLVREKFTFSKSLSTPPVILGCAQASTENRAAFKPDAG----GSGQ 215
            YS  Y D +  +G L +EKFT + S     V  GC +    N+  F   AG    G G+
Sbjct: 218 VYSIVYGDKSFTQGFLAKEKFTLTNSDVLEDVYFGCGE---NNQGLFDGVAGLLGLGPGK 272

Query: 216 TMIDSGSDLTY 219
             + + +  TY
Sbjct: 278 LSLPAQTTTTY 272

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BLAST of Csa3G188350 vs. Swiss-Prot
Match: ASPG1_ARATH (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 1.3e-09
Identity = 43/132 (32.58%), Postives = 57/132 (43.18%), Query Frame = 1


Query: 72  IGTPPQPTDLVLDTGSQLSWIQCHDKKVKKRLPPLPKPKTASFDPSLSSSFSLLPCNHPI 131
           +GTP +   LVLDTGS ++WIQC      +      +     F+P+ SS++  L C+ P 
Sbjct: 168 VGTPAKEMYLVLDTGSDVNWIQC------EPCADCYQQSDPVFNPTSSSTYKSLTCSAPQ 227

Query: 132 CKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSKSLSTPPVILGCAQAS 191
           C        L TS  ++  C Y   Y DG+   G L  +  TF  S     V LGC    
Sbjct: 228 CS------LLETSACRSNKCLYQVSYGDGSFTVGELATDTVTFGNSGKINNVALGCGH-- 284

Query: 192 TENRAAFKPDAG 204
            +N   F   AG
Sbjct: 288 -DNEGLFTGAAG 284

HSP 2 Score: 60.5 bits (145), Expect = 4.3e-08
Identity = 38/143 (26.57%), Postives = 65/143 (45.45%), Query Frame = 1


Query: 196 AAFKPDAGGSGQTMIDSGSDLTYLVDEAYEKVKEEVVRLVGAMMKKGYVYADVADMCFDA 255
           A F  DA GSG  ++D G+ +T L  +AY  +++  ++L    +KKG     + D C+D 
Sbjct: 364 AIFDVDASGSGGVILDCGTAVTRLQTQAYNSLRDAFLKLT-VNLKKGSSSISLFDTCYDF 423

Query: 256 GVTAEVGRRIGGISFEFDNGVEIFVGRGEGVLTEVEKGVKCVGIGRSERLGIGSNIIGTV 315
              + V  ++  ++F F  G  + +     ++   + G  C     +       +IIG V
Sbjct: 424 SSLSTV--KVPTVAFHFTGGKSLDLPAKNYLIPVDDSGTFCFAFAPTSS---SLSIIGNV 483

Query: 316 HQQNMWVEYDLANKRVGFGGAEC 339
            QQ   + YDL+   +G  G +C
Sbjct: 484 QQQGTRITYDLSKNVIGLSGNKC 500

[Close]

BLAST of Csa3G188350 vs. Swiss-Prot
Match: ASPG2_ARATH (Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana GN=ASPG2 PE=2 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 2.9e-09
Identity = 44/147 (29.93%), Postives = 65/147 (44.22%), Query Frame = 1


Query: 68  VSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKVKKRLPPLPKPKTASFDPSLSSSFSLLPC 127
           V + +G+PP+   +V+D+GS + W+QC   K+        K     FDP+ S S++ + C
Sbjct: 133 VRIGVGSPPRDQYMVIDSGSDMVWVQCQPCKL------CYKQSDPVFDPAKSGSYTGVSC 192

Query: 128 NHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSKSLSTPPVILGC 187
              +C     D    + C     C Y   Y DG+  +G L  E  TF+K++    V +GC
Sbjct: 193 GSSVC-----DRIENSGCHSGG-CRYEVMYGDGSYTKGTLALETLTFAKTV-VRNVAMGC 252

Query: 188 AQASTENRAAFKPDAGGSGQTMIDSGS 215
                 NR  F    G +G   I  GS
Sbjct: 253 GH---RNRGMF---IGAAGLLGIGGGS 260

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BLAST of Csa3G188350 vs. TrEMBL
Match: A0A0A0L6V5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G188350 PE=3 SV=1)

HSP 1 Score: 690.6 bits (1781), Expect = 9.3e-196
Identity = 342/342 (100.00%), Postives = 342/342 (100.00%), Query Frame = 1


Query: 1   MLLILFSLSLFTLSFSQSNSLSLPFPLSLSEKPSNTIPSYSSQLYAKRPSSYGSFKLPFK 60
           MLLILFSLSLFTLSFSQSNSLSLPFPLSLSEKPSNTIPSYSSQLYAKRPSSYGSFKLPFK
Sbjct: 1   MLLILFSLSLFTLSFSQSNSLSLPFPLSLSEKPSNTIPSYSSQLYAKRPSSYGSFKLPFK 60

Query: 61  YSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKVKKRLPPLPKPKTASFDPSLSS 120
           YSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKVKKRLPPLPKPKTASFDPSLSS
Sbjct: 61  YSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKVKKRLPPLPKPKTASFDPSLSS 120

Query: 121 SFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSKSLST 180
           SFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSKSLST
Sbjct: 121 SFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSKSLST 180

Query: 181 PPVILGCAQASTENRAAFKPDAGGSGQTMIDSGSDLTYLVDEAYEKVKEEVVRLVGAMMK 240
           PPVILGCAQASTENRAAFKPDAGGSGQTMIDSGSDLTYLVDEAYEKVKEEVVRLVGAMMK
Sbjct: 181 PPVILGCAQASTENRAAFKPDAGGSGQTMIDSGSDLTYLVDEAYEKVKEEVVRLVGAMMK 240

Query: 241 KGYVYADVADMCFDAGVTAEVGRRIGGISFEFDNGVEIFVGRGEGVLTEVEKGVKCVGIG 300
           KGYVYADVADMCFDAGVTAEVGRRIGGISFEFDNGVEIFVGRGEGVLTEVEKGVKCVGIG
Sbjct: 241 KGYVYADVADMCFDAGVTAEVGRRIGGISFEFDNGVEIFVGRGEGVLTEVEKGVKCVGIG 300

Query: 301 RSERLGIGSNIIGTVHQQNMWVEYDLANKRVGFGGAECSRLK 343
           RSERLGIGSNIIGTVHQQNMWVEYDLANKRVGFGGAECSRLK
Sbjct: 301 RSERLGIGSNIIGTVHQQNMWVEYDLANKRVGFGGAECSRLK 342

[Close]

BLAST of Csa3G188350 vs. TrEMBL
Match: A0A0A0LBQ0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G188340 PE=3 SV=1)

HSP 1 Score: 655.6 bits (1690), Expect = 3.3e-185
Identity = 326/343 (95.04%), Postives = 331/343 (96.50%), Query Frame = 1


Query: 1   MLLILFSLSLFTLSFSQSNSLSLPFPLSLSEKPSNTIPSY-SSQLYAKRPSSYGSFKLPF 60
           MLLILFSLSLFTLSFSQSNSLSLPFPLSL+EKPSN  P Y SSQLY K+PSS+G FKLPF
Sbjct: 20  MLLILFSLSLFTLSFSQSNSLSLPFPLSLTEKPSNITPLYYSSQLYVKKPSSHGPFKLPF 79

Query: 61  KYSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKVKKRLPPLPKPKTASFDPSLS 120
           KYSS+ALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKVKKRLPPLPKPKTASFDPSLS
Sbjct: 80  KYSSSALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKVKKRLPPLPKPKTASFDPSLS 139

Query: 121 SSFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSKSLS 180
           SSFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFS SLS
Sbjct: 140 SSFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSNSLS 199

Query: 181 TPPVILGCAQASTENRAAFKPDAGGSGQTMIDSGSDLTYLVDEAYEKVKEEVVRLVGAMM 240
           TPPVILGCAQ STENRAAFKPDAGGSGQTMIDSGSDLTYLVDEAYEKVKEEVVRLVGAMM
Sbjct: 200 TPPVILGCAQGSTENRAAFKPDAGGSGQTMIDSGSDLTYLVDEAYEKVKEEVVRLVGAMM 259

Query: 241 KKGYVYADVADMCFDAGVTAEVGRRIGGISFEFDNGVEIFVGRGEGVLTEVEKGVKCVGI 300
           KKGYVYA VADMCFDAGVT EVGRRIG +SFEFDNGVEIFVGRGEGVLTEVEKGVKCVGI
Sbjct: 260 KKGYVYAAVADMCFDAGVTVEVGRRIGDMSFEFDNGVEIFVGRGEGVLTEVEKGVKCVGI 319

Query: 301 GRSERLGIGSNIIGTVHQQNMWVEYDLANKRVGFGGAECSRLK 343
           GRS RLGIGSNIIGTVHQQNMWVEYDLANKRVGFGGAECSRLK
Sbjct: 320 GRSGRLGIGSNIIGTVHQQNMWVEYDLANKRVGFGGAECSRLK 362

[Close]

BLAST of Csa3G188350 vs. TrEMBL
Match: A0A087GTV5_ARAAL (Uncharacterized protein OS=Arabis alpina GN=AALP_AA6G358100 PE=3 SV=1)

HSP 1 Score: 386.7 bits (992), Expect = 2.9e-104
Identity = 210/359 (58.50%), Postives = 248/359 (69.08%), Query Frame = 1


Query: 1   MLLILFSLSLFTL-SFSQSNSLSLPFPLSLSEKPSNTIPSYSSQLYAKRPSSYGSFKLPF 60
           M L+L  + +F   S S S+S SL FPL  +  P+     + S L +  PS   SF+  F
Sbjct: 1   MSLVLTLVYIFLCNSLSLSSSYSLHFPLRRT--PTTNSSFFQSSLLSS-PSPI-SFRSNF 60

Query: 61  KYSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKVKKRLPPLPKPKTASFDPSLS 120
           KYS  AL++SLPIGTP QP +LVLDTGSQLSWIQC+ K          KP T SFDPS S
Sbjct: 61  KYS-VALIISLPIGTPSQPQELVLDTGSQLSWIQCNTKN--------KKPTTTSFDPSSS 120

Query: 121 SSFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSKSLS 180
           SSFS L C+HP+CKPRIPDFTLPTSCD N+LCHYSYFYADGT  EG LV+EKFTFS +  
Sbjct: 121 SSFSNLLCSHPLCKPRIPDFTLPTSCDTNKLCHYSYFYADGTFTEGKLVKEKFTFSNNQI 180

Query: 181 TPPVILGCAQASTENR----------------AAFKPDAGGSGQTMIDSGSDLTYLVDEA 240
           TPP+ILGCA  S++N+                + F+PDAGGSGQTMIDSGS+ TYLVD A
Sbjct: 181 TPPLILGCATESSDNKGILGIKIGQKRLNISSSVFRPDAGGSGQTMIDSGSEFTYLVDVA 240

Query: 241 YEKVKEEVVRLVGAMMKKGYVYADVADMCFDAGVTAEVGRRIGGISFEFDNGVEIFVGRG 300
           Y+KVKEE+V+LVG  +KKGY+Y   ADMCFD     E+GR IG + FEF +GVEI V + 
Sbjct: 241 YDKVKEEIVKLVGHRLKKGYMYGATADMCFDGNNPMEIGRLIGDLVFEFGSGVEIVVVK- 300

Query: 301 EGVLTEVEKGVKCVGIGRSERLGIGSNIIGTVHQQNMWVEYDLANKRVGFGGAECSRLK 343
           E VL  V  G+ CVGIGRS  LG  SNIIG VHQQN+WVE+DL N+RVGF   ECS LK
Sbjct: 301 ERVLVNVGGGIHCVGIGRSSMLGAASNIIGNVHQQNLWVEFDLTNRRVGFSKTECSGLK 345

[Close]

BLAST of Csa3G188350 vs. TrEMBL
Match: D7KTL4_ARALL (Predicted protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_676033 PE=3 SV=1)

HSP 1 Score: 282.3 bits (721), Expect = 7.7e-73
Identity = 169/346 (48.84%), Postives = 203/346 (58.67%), Query Frame = 1


Query: 3   LILFSLSLFT-LSFSQSNSLSLPFPLSLSEKPSNTIPSYSSQLYAKRPSSYG---SFKLP 62
           L  F   LF  +S S S SL LP         +N+    +S L  K PS      +F+  
Sbjct: 8   LFFFFFFLFNYVSLSSSLSLHLPLTSLPISSTTNSHRFTTSLLSRKNPSPSSPPYNFRSR 67

Query: 63  FKYSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKVKKRLPPLPKPKTASFDPSL 122
           FKYS  AL++SLPIGTPPQ   +VLDTGSQLSWIQCH    +K+LPP  KPKT SFDPSL
Sbjct: 68  FKYSM-ALIISLPIGTPPQAQQMVLDTGSQLSWIQCH----RKKLPP--KPKT-SFDPSL 127

Query: 123 SSSFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSKSL 182
           SSSFS LPC+HP+CKPRIPDFTLPTSCD NRLCHYSYFYADGT AEGNLV+EK TFS + 
Sbjct: 128 SSSFSTLPCSHPLCKPRIPDFTLPTSCDSNRLCHYSYFYADGTFAEGNLVKEKITFSNTE 187

Query: 183 STPPVILGCAQASTENRAAFKPDAGGSGQTMIDSGSDLTYLVDEAYEK---VKEEVVRLV 242
            TPP+ILGCA  S+++R     + G          +  +Y +     +          L 
Sbjct: 188 ITPPLILGCATESSDDRGILGMNRGRLSFVSQAKITKFSYCIPPKSNRPGFTPTGSFYLG 247

Query: 243 GAMMKKGYVYADVADMCFDAGVTAEVGRRIGGISFEFDNGVEIFVGRGEGVLTEVEKGVK 302
                KG+ Y  +                       F   VEI V + E VL  V  G+ 
Sbjct: 248 DNPNSKGFKYVSL---------------------LTFPERVEILVPK-ERVLVNVGDGIH 307

Query: 303 CVGIGRSERLGIGSNIIGTVHQQNMWVEYDLANKRVGFGGAECSRL 342
           CVGIGRS  LG  SNIIG VHQQN+WVE+D+ N+RVGF  A+CSR+
Sbjct: 308 CVGIGRSSMLGAASNIIGNVHQQNLWVEFDVTNRRVGFARADCSRI 323

[Close]

BLAST of Csa3G188350 vs. TrEMBL
Match: Q9FGI3_ARATH (AT5g37540/mpa22_p_70 OS=Arabidopsis thaliana GN=At5g37540 PE=2 SV=1)

HSP 1 Score: 244.6 bits (623), Expect = 1.8e-61
Identity = 128/207 (61.84%), Postives = 153/207 (73.91%), Query Frame = 1


Query: 2   LLILFSLSLFTLSFSQSNSLSLPFPL-SLSEKPSNTIPSYSSQLYAKR----PSSYGSFK 61
           LL +F    +++S S S+SLSL FPL SL   P+    S+ + L ++R    PSS  +F+
Sbjct: 12  LLYIFFFFCYSVSLSWSSSLSLHFPLTSLRLTPTTNSSSFKTSLLSRRNPSPPSSPYTFR 71

Query: 62  LPFKYSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKVKKRLPPLPKPKTASFDP 121
              KYS  AL++SLPIGTP Q  +LVLDTGSQLSWIQCH KK+KK LPP     T SFDP
Sbjct: 72  SNIKYSM-ALILSLPIGTPSQSQELVLDTGSQLSWIQCHPKKIKKPLPP----PTTSFDP 131

Query: 122 SLSSSFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSK 181
           SLSSSFS LPC+HP+CKPRIPDFTLPTSCD NRLCHYSYFYADGT AEGNLV+EKFTFS 
Sbjct: 132 SLSSSFSDLPCSHPLCKPRIPDFTLPTSCDSNRLCHYSYFYADGTFAEGNLVKEKFTFSN 191

Query: 182 SLSTPPVILGCAQASTENRAAFKPDAG 204
           S +TPP+ILGCA+ ST+ +     + G
Sbjct: 192 SQTTPPLILGCAKESTDEKGILGMNLG 213

[Close]

BLAST of Csa3G188350 vs. TAIR10
Match: AT5G37540.1 (AT5G37540.1 Eukaryotic aspartyl protease family protein)

HSP 1 Score: 244.6 bits (623), Expect = 9.0e-65
Identity = 128/207 (61.84%), Postives = 151/207 (72.95%), Query Frame = 1


Query: 2   LLILFSLSLFTLSFSQSNSLSLPFPL-SLSEKPSNTIPSYSSQLYAKR----PSSYGSFK 61
           LL +F    +++S S S+SLSL FPL SL   P+    S+ + L ++R    PSS  +F+
Sbjct: 12  LLYIFFFFCYSVSLSWSSSLSLHFPLTSLRLTPTTNSSSFKTSLLSRRNPSPPSSPYTFR 71

Query: 62  LPFKYSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKVKKRLPPLPKPKTASFDP 121
              KYS  AL++SLPIGTP Q  +LVLDTGSQLSWIQCH KK+KK LPP     T SFDP
Sbjct: 72  SNIKYSM-ALILSLPIGTPSQSQELVLDTGSQLSWIQCHPKKIKKPLPP----PTTSFDP 131

Query: 122 SLSSSFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSK 181
           SLSSSFS LPC+HP+CKPRIPDFTLPTSCD NRLCHYSYFYADGT AEGNLV+EKFTFS 
Sbjct: 132 SLSSSFSDLPCSHPLCKPRIPDFTLPTSCDSNRLCHYSYFYADGTFAEGNLVKEKFTFSN 191

Query: 182 SLSTPPVILGCAQASTENRAAFKPDAG 204
           S +TPP+ILGCA+ ST+ +     + G
Sbjct: 192 SQTTPPLILGCAKESTDEKGILGMNLG 213

HSP 2 Score: 190.7 bits (483), Expect = 1.5e-48
Identity = 91/146 (62.33%), Postives = 109/146 (74.66%), Query Frame = 1


Query: 196 AAFKPDAGGSGQTMIDSGSDLTYLVDEAYEKVKEEVVRLVGAMMKKGYVYADVADMCFDA 255
           + F+PDAGGSGQTM+DSGS+ T+LVD AY+KVKEE+VRLVG+ +KKGYVY   ADMCFD 
Sbjct: 297 SVFRPDAGGSGQTMVDSGSEFTHLVDVAYDKVKEEIVRLVGSRLKKGYVYGSTADMCFDG 356

Query: 256 GVTAEVGRRIGGISFEFDNGVEIFVGRGEGVLTEVEKGVKCVGIGRSERLGIGSNIIGTV 315
             + E+GR IG + FEF  GVEI V + + +L  V  G+ CVGIGRS  LG  SNIIG V
Sbjct: 357 NHSMEIGRLIGDLVFEFGRGVEILVEK-QSLLVNVGGGIHCVGIGRSSMLGAASNIIGNV 416

Query: 316 HQQNMWVEYDLANKRVGFGGAECSRL 342
           HQQN+WVE+D+ N+RVGF  AEC  L
Sbjct: 417 HQQNLWVEFDVTNRRVGFSKAECRLL 441

[Close]

BLAST of Csa3G188350 vs. TAIR10
Match: AT1G66180.1 (AT1G66180.1 Eukaryotic aspartyl protease family protein)

HSP 1 Score: 219.9 bits (559), Expect = 2.4e-57
Identity = 120/203 (59.11%), Postives = 139/203 (68.47%), Query Frame = 1


Query: 4   ILFSLSLFTLSFSQSNSLSLPFPLSLSEKPSNTIPSYSSQLYAKRPSSYG---SFKLPFK 63
           + F   L  +S S S SL LP         +N+    +S L  K PS      +F+  FK
Sbjct: 8   LFFFFFLNYVSLSTSLSLHLPLTSLPISTTTNSHRFTTSLLSRKNPSPSSPPYNFRSRFK 67

Query: 64  YSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKVKKRLPPLPKPKTASFDPSLSS 123
           YS  AL++SLPIGTPPQ   +VLDTGSQLSWIQCH    +K+LPP  KPKT SFDPSLSS
Sbjct: 68  YSM-ALIISLPIGTPPQAQQMVLDTGSQLSWIQCH----RKKLPP--KPKT-SFDPSLSS 127

Query: 124 SFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSKSLST 183
           SFS LPC+HP+CKPRIPDFTLPTSCD NRLCHYSYFYADGT AEGNLV+EK TFS +  T
Sbjct: 128 SFSTLPCSHPLCKPRIPDFTLPTSCDSNRLCHYSYFYADGTFAEGNLVKEKITFSNTEIT 187

Query: 184 PPVILGCAQASTENRAAFKPDAG 204
           PP+ILGCA  S+++R     + G
Sbjct: 188 PPLILGCATESSDDRGILGMNRG 202

HSP 2 Score: 175.6 bits (444), Expect = 5.1e-44
Identity = 87/146 (59.59%), Postives = 105/146 (71.92%), Query Frame = 1


Query: 196 AAFKPDAGGSGQTMIDSGSDLTYLVDEAYEKVKEEVVRLVGAMMKKGYVYADVADMCFDA 255
           + F+PDAGGSGQTM+DSGS+ T+LVD AY+KV+ E++  VG  +KKGYVY   ADMCFD 
Sbjct: 286 SVFRPDAGGSGQTMVDSGSEFTHLVDAAYDKVRAEIMTRVGRRLKKGYVYGGTADMCFDG 345

Query: 256 GVTAEVGRRIGGISFEFDNGVEIFVGRGEGVLTEVEKGVKCVGIGRSERLGIGSNIIGTV 315
            V A + R IG + F F  GVEI V + E VL  V  G+ CVGIGRS  LG  SNIIG V
Sbjct: 346 NV-AMIPRLIGDLVFVFTRGVEILVPK-ERVLVNVGGGIHCVGIGRSSMLGAASNIIGNV 405

Query: 316 HQQNMWVEYDLANKRVGFGGAECSRL 342
           HQQN+WVE+D+ N+RVGF  A+CSR+
Sbjct: 406 HQQNLWVEFDVTNRRVGFAKADCSRV 429

[Close]

BLAST of Csa3G188350 vs. TAIR10
Match: AT5G02190.1 (AT5G02190.1 Eukaryotic aspartyl protease family protein)

HSP 1 Score: 118.2 bits (295), Expect = 9.7e-27
Identity = 71/182 (39.01%), Postives = 100/182 (54.95%), Query Frame = 1


Query: 6   FSLSLFTLSFSQSNSLSLPFPLSLSEKPSNTIPSYSSQLYAKRPSSYGSFKLPFKYSSTA 65
           FS S F+   S S+S +L  PL     P++            RP+     KL F ++ T 
Sbjct: 32  FSFSSFS---SSSSSQTLVLPLKTRITPTD-----------HRPTD----KLHFHHNVT- 91

Query: 66  LVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKVKKRLPPLPKPKTASFDPSLSSSFSLL 125
           L V+L +GTPPQ   +V+DTGS+LSW++C+           P P   +FDP+ SSS+S +
Sbjct: 92  LTVTLTVGTPPQNISMVIDTGSELSWLRCNRSSN-------PNPVN-NFDPTRSSSYSPI 151

Query: 126 PCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSKSLSTPPVIL 185
           PC+ P C+ R  DF +P SCD ++LCH +  YAD + +EGNL  E F F  S +   +I 
Sbjct: 152 PCSSPTCRTRTRDFLIPASCDSDKLCHATLSYADASSSEGNLAAEIFHFGNSTNDSNLIF 186

Query: 186 GC 188
           GC
Sbjct: 212 GC 186

HSP 2 Score: 78.6 bits (192), Expect = 8.5e-15
Identity = 46/157 (29.30%), Postives = 76/157 (48.41%), Query Frame = 1


Query: 195 RAAFKPDAGGSGQTMIDSGSDLTYLVDEAYEKVKEEVVRLVGAMMK----KGYVYADVAD 254
           ++   PD  G+GQTM+DSG+  T+L+   Y  ++   +     ++       +V+    D
Sbjct: 288 KSVLVPDHTGAGQTMVDSGTQFTFLLGPVYTALRSHFLNRTNGILTVYEDPDFVFQGTMD 347

Query: 255 MCFDAG---VTAEVGRRIGGISFEFDNGVEIFVGRGEGVLTEV------EKGVKCVGIGR 314
           +C+      + + +  R+  +S  F+ G EI V  G+ +L  V         V C   G 
Sbjct: 348 LCYRISPVRIRSGILHRLPTVSLVFE-GAEIAVS-GQPLLYRVPHLTVGNDSVYCFTFGN 407

Query: 315 SERLGIGSNIIGTVHQQNMWVEYDLANKRVGFGGAEC 339
           S+ +G+ + +IG  HQQNMW+E+DL   R+G    EC
Sbjct: 408 SDLMGMEAYVIGHHHQQNMWIEFDLQRSRIGLAPVEC 442

[Close]

BLAST of Csa3G188350 vs. TAIR10
Match: AT2G39710.1 (AT2G39710.1 Eukaryotic aspartyl protease family protein)

HSP 1 Score: 114.0 bits (284), Expect = 1.8e-25
Identity = 78/209 (37.32%), Postives = 111/209 (53.11%), Query Frame = 1


Query: 7   SLSLFTLSFSQSNSLSLPFPLSLSEKPSNTIPSYSSQLYAKRPSSYGSFKLPFKYSSTAL 66
           SLSL + +F + + L L FPL+  +  S       S    K P S  S KL F+++ T L
Sbjct: 9   SLSL-SKNFLRISVLLLIFPLTFCKTSSTNQTLLFSLKTQKLPQS-SSDKLSFRHNVT-L 68

Query: 67  VVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKVKKRLPPLPKPKTASFDPSLSSSFSLLP 126
            V+L +G PPQ   +VLDTGS+LSW+ C      K+ P L     + F+P  SS++S +P
Sbjct: 69  TVTLAVGDPPQNISMVLDTGSELSWLHC------KKSPNL----GSVFNPVSSSTYSPVP 128

Query: 127 CNHPICKPRIPDFTLPTSCD-QNRLCHYSYFYADGTLAEGNLVREKFTFSKSLSTPPVIL 186
           C+ PIC+ R  D  +P SCD +  LCH +  YAD T  EGNL  E F    S++ P  + 
Sbjct: 129 CSSPICRTRTRDLPIPASCDPKTHLCHVAISYADATSIEGNLAHETFVIG-SVTRPGTLF 188

Query: 187 GCAQASTENRAAFKPDAGGSGQTMIDSGS 215
           GC  +   + +  + DA  +G   ++ GS
Sbjct: 189 GCMDSGLSSNS--EEDAKSTGLMGMNRGS 201

HSP 2 Score: 88.2 bits (217), Expect = 1.1e-17
Identity = 52/152 (34.21%), Postives = 79/152 (51.97%), Query Frame = 1


Query: 195 RAAFKPDAGGSGQTMIDSGSDLTYLVDEAYEKVKEEVVRLVGAMMK----KGYVYADVAD 254
           ++ F PD  G+GQTM+DSG+  T+L+   Y  +K E +    ++++      +V+    D
Sbjct: 277 KSVFVPDHTGAGQTMVDSGTQFTFLMGPVYTALKNEFITQTKSVLRLVDDPDFVFQGTMD 336

Query: 255 MCFDAGVTAEVG-RRIGGISFEFDNGVEIFVG------RGEGVLTEVEKGVKCVGIGRSE 314
           +C+  G T       +  +S  F  G E+ V       R  G  +E ++ V C   G S+
Sbjct: 337 LCYKVGSTTRPNFSGLPMVSLMF-RGAEMSVSGQKLLYRVNGAGSEGKEEVYCFTFGNSD 396

Query: 315 RLGIGSNIIGTVHQQNMWVEYDLANKRVGFGG 336
            LGI + +IG  HQQN+W+E+DLA  RVGF G
Sbjct: 397 LLGIEAFVIGHHHQQNVWMEFDLAKSRVGFAG 427

[Close]

BLAST of Csa3G188350 vs. TAIR10
Match: AT5G10760.1 (AT5G10760.1 Eukaryotic aspartyl protease family protein)

HSP 1 Score: 75.9 bits (185), Expect = 5.5e-14
Identity = 60/191 (31.41%), Postives = 84/191 (43.98%), Query Frame = 1


Query: 36  TIPSYSSQLYAKRPSSYGSFKLPFKYSSTA----LVVSLPIGTPPQPTDLVLDTGSQLSW 95
           +I S  S+  A   S   S +LP K   T      +V++ IGTP     LV DTGS L+W
Sbjct: 98  SIYSKLSKNSANEVSEAKSTELPAKSGITLGSGNYIVTIGIGTPKHDLSLVFDTGSDLTW 157

Query: 96  IQCHDKKVKKRLPPLPKPKTASFDPSLSSSFSLLPCNHPICKPRIPDFTLPTSCDQNRLC 155
            QC     +  L      K   F+PS SS++  + C+ P+C+          SC  +  C
Sbjct: 158 TQC-----EPCLGSCYSQKEPKFNPSSSSTYQNVSCSSPMCED-------AESCSASN-C 217

Query: 156 HYSYFYADGTLAEGNLVREKFTFSKSLSTPPVILGCAQASTENRAAFKPDAG----GSGQ 215
            YS  Y D +  +G L +EKFT + S     V  GC +    N+  F   AG    G G+
Sbjct: 218 VYSIVYGDKSFTQGFLAKEKFTLTNSDVLEDVYFGCGE---NNQGLFDGVAGLLGLGPGK 272

Query: 216 TMIDSGSDLTY 219
             + + +  TY
Sbjct: 278 LSLPAQTTTTY 272

[Close]

BLAST of Csa3G188350 vs. NCBI nr
Match: gi|700202330|gb|KGN57463.1| (hypothetical protein Csa_3G188350 [Cucumis sativus])

HSP 1 Score: 690.6 bits (1781), Expect = 1.3e-195
Identity = 342/342 (100.00%), Postives = 342/342 (100.00%), Query Frame = 1


Query: 1   MLLILFSLSLFTLSFSQSNSLSLPFPLSLSEKPSNTIPSYSSQLYAKRPSSYGSFKLPFK 60
           MLLILFSLSLFTLSFSQSNSLSLPFPLSLSEKPSNTIPSYSSQLYAKRPSSYGSFKLPFK
Sbjct: 1   MLLILFSLSLFTLSFSQSNSLSLPFPLSLSEKPSNTIPSYSSQLYAKRPSSYGSFKLPFK 60

Query: 61  YSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKVKKRLPPLPKPKTASFDPSLSS 120
           YSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKVKKRLPPLPKPKTASFDPSLSS
Sbjct: 61  YSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKVKKRLPPLPKPKTASFDPSLSS 120

Query: 121 SFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSKSLST 180
           SFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSKSLST
Sbjct: 121 SFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSKSLST 180

Query: 181 PPVILGCAQASTENRAAFKPDAGGSGQTMIDSGSDLTYLVDEAYEKVKEEVVRLVGAMMK 240
           PPVILGCAQASTENRAAFKPDAGGSGQTMIDSGSDLTYLVDEAYEKVKEEVVRLVGAMMK
Sbjct: 181 PPVILGCAQASTENRAAFKPDAGGSGQTMIDSGSDLTYLVDEAYEKVKEEVVRLVGAMMK 240

Query: 241 KGYVYADVADMCFDAGVTAEVGRRIGGISFEFDNGVEIFVGRGEGVLTEVEKGVKCVGIG 300
           KGYVYADVADMCFDAGVTAEVGRRIGGISFEFDNGVEIFVGRGEGVLTEVEKGVKCVGIG
Sbjct: 241 KGYVYADVADMCFDAGVTAEVGRRIGGISFEFDNGVEIFVGRGEGVLTEVEKGVKCVGIG 300

Query: 301 RSERLGIGSNIIGTVHQQNMWVEYDLANKRVGFGGAECSRLK 343
           RSERLGIGSNIIGTVHQQNMWVEYDLANKRVGFGGAECSRLK
Sbjct: 301 RSERLGIGSNIIGTVHQQNMWVEYDLANKRVGFGGAECSRLK 342

[Close]

BLAST of Csa3G188350 vs. NCBI nr
Match: gi|700202328|gb|KGN57461.1| (hypothetical protein Csa_3G188340 [Cucumis sativus])

HSP 1 Score: 655.6 bits (1690), Expect = 4.8e-185
Identity = 326/343 (95.04%), Postives = 331/343 (96.50%), Query Frame = 1


Query: 1   MLLILFSLSLFTLSFSQSNSLSLPFPLSLSEKPSNTIPSY-SSQLYAKRPSSYGSFKLPF 60
           MLLILFSLSLFTLSFSQSNSLSLPFPLSL+EKPSN  P Y SSQLY K+PSS+G FKLPF
Sbjct: 20  MLLILFSLSLFTLSFSQSNSLSLPFPLSLTEKPSNITPLYYSSQLYVKKPSSHGPFKLPF 79

Query: 61  KYSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKVKKRLPPLPKPKTASFDPSLS 120
           KYSS+ALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKVKKRLPPLPKPKTASFDPSLS
Sbjct: 80  KYSSSALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKVKKRLPPLPKPKTASFDPSLS 139

Query: 121 SSFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSKSLS 180
           SSFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFS SLS
Sbjct: 140 SSFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSNSLS 199

Query: 181 TPPVILGCAQASTENRAAFKPDAGGSGQTMIDSGSDLTYLVDEAYEKVKEEVVRLVGAMM 240
           TPPVILGCAQ STENRAAFKPDAGGSGQTMIDSGSDLTYLVDEAYEKVKEEVVRLVGAMM
Sbjct: 200 TPPVILGCAQGSTENRAAFKPDAGGSGQTMIDSGSDLTYLVDEAYEKVKEEVVRLVGAMM 259

Query: 241 KKGYVYADVADMCFDAGVTAEVGRRIGGISFEFDNGVEIFVGRGEGVLTEVEKGVKCVGI 300
           KKGYVYA VADMCFDAGVT EVGRRIG +SFEFDNGVEIFVGRGEGVLTEVEKGVKCVGI
Sbjct: 260 KKGYVYAAVADMCFDAGVTVEVGRRIGDMSFEFDNGVEIFVGRGEGVLTEVEKGVKCVGI 319

Query: 301 GRSERLGIGSNIIGTVHQQNMWVEYDLANKRVGFGGAECSRLK 343
           GRS RLGIGSNIIGTVHQQNMWVEYDLANKRVGFGGAECSRLK
Sbjct: 320 GRSGRLGIGSNIIGTVHQQNMWVEYDLANKRVGFGGAECSRLK 362

[Close]

BLAST of Csa3G188350 vs. NCBI nr
Match: gi|778679913|ref|XP_004140731.2| (PREDICTED: aspartic proteinase PCS1-like [Cucumis sativus])

HSP 1 Score: 398.7 bits (1023), Expect = 1.1e-107
Identity = 196/203 (96.55%), Postives = 197/203 (97.04%), Query Frame = 1


Query: 1   MLLILFSLSLFTLSFSQSNSLSLPFPLSLSEKPSNTIPSYSSQLYAKRPSSYGSFKLPFK 60
           MLLILFSLSLFTLSFSQSNSLSLPFPLSLSEKPSNTIPSYSSQLYAKRPSSYGSFKLPFK
Sbjct: 1   MLLILFSLSLFTLSFSQSNSLSLPFPLSLSEKPSNTIPSYSSQLYAKRPSSYGSFKLPFK 60

Query: 61  YSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKVKKRLPPLPKPKTASFDPSLSS 120
           YSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKVKKRLPPLPKPKTASFDPSLSS
Sbjct: 61  YSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKVKKRLPPLPKPKTASFDPSLSS 120

Query: 121 SFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSKSLST 180
           SFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSKSLST
Sbjct: 121 SFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSKSLST 180

Query: 181 PPVILGCAQASTENRAAFKPDAG 204
           PPVILGCAQASTENR     + G
Sbjct: 181 PPVILGCAQASTENRGILGMNRG 203

[Close]

BLAST of Csa3G188350 vs. NCBI nr
Match: gi|778679913|ref|XP_004140731.2| (PREDICTED: aspartic proteinase PCS1-like [Cucumis sativus])

HSP 1 Score: 297.0 bits (759), Expect = 4.3e-77
Identity = 147/147 (100.00%), Postives = 147/147 (100.00%), Query Frame = 1


Query: 196 AAFKPDAGGSGQTMIDSGSDLTYLVDEAYEKVKEEVVRLVGAMMKKGYVYADVADMCFDA 255
           AAFKPDAGGSGQTMIDSGSDLTYLVDEAYEKVKEEVVRLVGAMMKKGYVYADVADMCFDA
Sbjct: 284 AAFKPDAGGSGQTMIDSGSDLTYLVDEAYEKVKEEVVRLVGAMMKKGYVYADVADMCFDA 343

Query: 256 GVTAEVGRRIGGISFEFDNGVEIFVGRGEGVLTEVEKGVKCVGIGRSERLGIGSNIIGTV 315
           GVTAEVGRRIGGISFEFDNGVEIFVGRGEGVLTEVEKGVKCVGIGRSERLGIGSNIIGTV
Sbjct: 344 GVTAEVGRRIGGISFEFDNGVEIFVGRGEGVLTEVEKGVKCVGIGRSERLGIGSNIIGTV 403

Query: 316 HQQNMWVEYDLANKRVGFGGAECSRLK 343
           HQQNMWVEYDLANKRVGFGGAECSRLK
Sbjct: 404 HQQNMWVEYDLANKRVGFGGAECSRLK 430

HSP 2 Score: 386.7 bits (992), Expect = 4.1e-104
Identity = 210/359 (58.50%), Postives = 248/359 (69.08%), Query Frame = 1


Query: 1   MLLILFSLSLFTL-SFSQSNSLSLPFPLSLSEKPSNTIPSYSSQLYAKRPSSYGSFKLPF 60
           M L+L  + +F   S S S+S SL FPL  +  P+     + S L +  PS   SF+  F
Sbjct: 1   MSLVLTLVYIFLCNSLSLSSSYSLHFPLRRT--PTTNSSFFQSSLLSS-PSPI-SFRSNF 60

Query: 61  KYSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKVKKRLPPLPKPKTASFDPSLS 120
           KYS  AL++SLPIGTP QP +LVLDTGSQLSWIQC+ K          KP T SFDPS S
Sbjct: 61  KYS-VALIISLPIGTPSQPQELVLDTGSQLSWIQCNTKN--------KKPTTTSFDPSSS 120

Query: 121 SSFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSKSLS 180
           SSFS L C+HP+CKPRIPDFTLPTSCD N+LCHYSYFYADGT  EG LV+EKFTFS +  
Sbjct: 121 SSFSNLLCSHPLCKPRIPDFTLPTSCDTNKLCHYSYFYADGTFTEGKLVKEKFTFSNNQI 180

Query: 181 TPPVILGCAQASTENR----------------AAFKPDAGGSGQTMIDSGSDLTYLVDEA 240
           TPP+ILGCA  S++N+                + F+PDAGGSGQTMIDSGS+ TYLVD A
Sbjct: 181 TPPLILGCATESSDNKGILGIKIGQKRLNISSSVFRPDAGGSGQTMIDSGSEFTYLVDVA 240

Query: 241 YEKVKEEVVRLVGAMMKKGYVYADVADMCFDAGVTAEVGRRIGGISFEFDNGVEIFVGRG 300
           Y+KVKEE+V+LVG  +KKGY+Y   ADMCFD     E+GR IG + FEF +GVEI V + 
Sbjct: 241 YDKVKEEIVKLVGHRLKKGYMYGATADMCFDGNNPMEIGRLIGDLVFEFGSGVEIVVVK- 300

Query: 301 EGVLTEVEKGVKCVGIGRSERLGIGSNIIGTVHQQNMWVEYDLANKRVGFGGAECSRLK 343
           E VL  V  G+ CVGIGRS  LG  SNIIG VHQQN+WVE+DL N+RVGF   ECS LK
Sbjct: 301 ERVLVNVGGGIHCVGIGRSSMLGAASNIIGNVHQQNLWVEFDLTNRRVGFSKTECSGLK 345

[Close]

BLAST of Csa3G188350 vs. NCBI nr
Match: gi|778679910|ref|XP_011651212.1| (PREDICTED: aspartic proteinase PCS1-like [Cucumis sativus])

HSP 1 Score: 374.4 bits (960), Expect = 2.1e-100
Identity = 184/196 (93.88%), Postives = 188/196 (95.92%), Query Frame = 1


Query: 1   MLLILFSLSLFTLSFSQSNSLSLPFPLSLSEKPSNTIPSY-SSQLYAKRPSSYGSFKLPF 60
           MLLILFSLSLFTLSFSQSNSLSLPFPLSL+EKPSN  P Y SSQLY K+PSS+G FKLPF
Sbjct: 1   MLLILFSLSLFTLSFSQSNSLSLPFPLSLTEKPSNITPLYYSSQLYVKKPSSHGPFKLPF 60

Query: 61  KYSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKVKKRLPPLPKPKTASFDPSLS 120
           KYSS+ALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKVKKRLPPLPKPKTASFDPSLS
Sbjct: 61  KYSSSALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKVKKRLPPLPKPKTASFDPSLS 120

Query: 121 SSFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSKSLS 180
           SSFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFS SLS
Sbjct: 121 SSFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSNSLS 180

Query: 181 TPPVILGCAQASTENR 196
           TPPVILGCAQ STENR
Sbjct: 181 TPPVILGCAQGSTENR 196

The following BLAST results are available for this feature:

BLAST of Csa3G188350 vs. Swiss-Prot


Match Name	E-value	Identity	Description
PCS1L_ARATH	1.7e-25	39.01	Aspartic proteinase PCS1 OS=Arabidopsis thaliana GN=PCS1 PE=2 SV=1	[more]
NEP2_NEPGR	7.5e-13	35.77	Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis GN=nep2 PE=1 SV=1	[more]
AED1_ARATH	9.8e-13	31.41	Aspartyl protease AED1 OS=Arabidopsis thaliana GN=AED1 PE=2 SV=1	[more]
ASPG1_ARATH	1.3e-09	32.58	Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana GN=ASPG1 PE=1 ...	[more]
ASPG2_ARATH	2.9e-09	29.93	Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana GN=ASPG2 PE=2 ...	[more]

BLAST of Csa3G188350 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L6V5_CUCSA	9.3e-196	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G188350 PE=3 SV=1	[more]
A0A0A0LBQ0_CUCSA	3.3e-185	95.04	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G188340 PE=3 SV=1	[more]
A0A087GTV5_ARAAL	2.9e-104	58.50	Uncharacterized protein OS=Arabis alpina GN=AALP_AA6G358100 PE=3 SV=1	[more]
D7KTL4_ARALL	7.7e-73	48.84	Predicted protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_676033 PE=3 ...	[more]
Q9FGI3_ARATH	1.8e-61	61.84	AT5g37540/mpa22_p_70 OS=Arabidopsis thaliana GN=At5g37540 PE=2 SV=1	[more]

BLAST of Csa3G188350 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G37540.1	9.0e-65	61.84	Eukaryotic aspartyl protease family protein	[more]
AT1G66180.1	2.4e-57	59.11	Eukaryotic aspartyl protease family protein	[more]
AT5G02190.1	9.7e-27	39.01	Eukaryotic aspartyl protease family protein	[more]
AT2G39710.1	1.8e-25	37.32	Eukaryotic aspartyl protease family protein	[more]
AT5G10760.1	5.5e-14	31.41	Eukaryotic aspartyl protease family protein	[more]

BLAST of Csa3G188350 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700202330\|gb\|KGN57463.1\|	1.3e-195	100.00	hypothetical protein Csa_3G188350 [Cucumis sativus]	[more]
gi\|700202328\|gb\|KGN57461.1\|	4.8e-185	95.04	hypothetical protein Csa_3G188340 [Cucumis sativus]	[more]
gi\|778679913\|ref\|XP_004140731.2\|	1.1e-107	96.55	PREDICTED: aspartic proteinase PCS1-like [Cucumis sativus]	[more]
gi\|778679913\|ref\|XP_004140731.2\|	4.3e-77	100.00	PREDICTED: aspartic proteinase PCS1-like [Cucumis sativus]	[more]
gi\|778679910\|ref\|XP_011651212.1\|	2.1e-100	93.88	PREDICTED: aspartic proteinase PCS1-like [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR001461	Aspartic_peptidase_A1
IPR001969	Aspartic_peptidase_AS
IPR021109	Peptidase_aspartic_dom_sf

Vocabulary: **Molecular Function**
Term	Definition
GO:0004190	aspartic-type endopeptidase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0006508	proteolysis

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004190	aspartic-type endopeptidase activity
molecular_function	GO:0016740	transferase activity

Unigene

This gene is associated with the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
CU164870	cucumber EST collection version 3.0	transcribed_cluster

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Csa3G188350.1	Csa3G188350.1	mRNA

The following transcribed_cluster feature(s) are associated with this gene:


Feature Name	Unique Name	Type
CU164870	CU164870	transcribed_cluster

InterPro

Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001461	Aspartic peptidase A1 family	PRINTS	PR00792	PEPSIN	coord: 72..92 score: 1.7E-5coord: 208..219 score: 1.7E-5coord: 310..325 score: 1.
IPR001461	Aspartic peptidase A1 family	PANTHER	PTHR13683	ASPARTYL PROTEASES	coord: 1..341 score: 4.7E
IPR001969	Aspartic peptidase, active site	PROSITE	PS00141	ASP_PROTEASE	coord: 81..92 scor
IPR021109	Aspartic peptidase domain	GENE3D	G3DSA:2.40.70.10		coord: 67..196 score: 8.9E-21coord: 197..340 score: 4.5
IPR021109	Aspartic peptidase domain	unknown	SSF50630	Acid proteases	coord: 62..340 score: 1.25
None	No IPR available	PANTHER	PTHR13683:SF327	ASPARTYL PROTEASE FAMILY PROTEIN	coord: 1..341 score: 4.7E

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Csa3G188350	Cucumber (Gy14) v1	cgycuB132
Csa3G188350	Cucurbita maxima (Rimu)	cmacuB245
Csa3G188350	Cucurbita maxima (Rimu)	cmacuB458
Csa3G188350	Cucurbita moschata (Rifu)	cmocuB232
Csa3G188350	Cucurbita moschata (Rifu)	cmocuB445
Csa3G188350	Melon (DHL92) v3.5.1	cumeB251
Csa3G188350	Watermelon (Charleston Gray)	cuwcgB230
Csa3G188350	Watermelon (97103) v1	cuwmB243
Csa3G188350	Cucurbita pepo (Zucchini)	cpecuB550
Csa3G188350	Cucurbita pepo (Zucchini)	cpecuB603
Csa3G188350	Bottle gourd (USVL1VR-Ls)	culsiB211
Csa3G188350	Cucumber (Gy14) v2	cgybcuB109
Csa3G188350	Melon (DHL92) v3.6.1	cumedB238
Csa3G188350	Silver-seed gourd	carcuB0196
Csa3G188350	Silver-seed gourd	carcuB0781
Csa3G188350	Watermelon (97103) v2	cuwmbB215
Csa3G188350	Wax gourd	cuwgoB283