Csa3G188340 (gene) Cucumber (Chinese Long) v2

Overview
NameCsa3G188340
Typegene
OrganismCucumis. sativus (Cucumber (Chinese Long) v2)
DescriptionAspartic proteinase nepenthesin-1; contains IPR001461 (Peptidase A1), IPR021109 (Aspartic peptidase)
LocationChr3 : 13265194 .. 13266696 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTATATAATCCCCAACCCTCCACTTCACCAATCCCCCCCCCCAACACAATCAACAATGCTTCTAATTCTCTTCTCTCTCTCATTATTCACTCTCTCCTTCTCTCAATCCAATTCCCTCTCTCTCCCCTTCCCTCTTTCTCTCACTGAAAAACCCTCCAATATTACCCCATTATACTACTCTTCCCAGCTTTACGTCAAAAAGCCATCATCCCATGGCCCCTTCAAGCTTCCTTTCAAATACTCCTCCTCTGCCCTCGTCGTCTCTCTTCCGATCGGAACGCCGCCACAGCCCACTGACTTGGTTCTAGACACCGGCAGCCAACTCTCTTGGATTCAATGTCATGACAAAAAAGTTAAGAAAAGATTGCCCCCCTTGCCCAAGCCTAAAACCGCCTCCTTTGATCCTTCTCTCTCCTCTTCTTTTTCTCTCCTCCCTTGTAATCACCCCATCTGCAAACCCCGAATTCCAGATTTTACCCTTCCCACTTCTTGTGACCAAAATCGCCTCTGCCACTACTCCTACTTCTACGCTGACGGTACCTTGGCTGAGGGAAATCTCGTCAGAGAAAAATTTACCTTCTCTAATTCTCTTTCTACCCCTCCCGTCATCCTCGGTTGCGCTCAAGGCTCCACCGAAAACAGGGGTATTTTGGGAATGAATCATGGACGTTTGTCCTTTATCTCCCAAGCTAAAATCTCCAAATTCTCCTATTGCGTTCCGAGTCGAACAGGGTCTAATCCCACCGGGCTATTCTACCTGGGAGATAACCCCAATTCTTCCAAATTCAAATACGTCACCATGTTGACTTTTCCTGAAAGTCAAAGCTCTCCGAATCTCGACCCACTGGCTTACACTCTCCCTATGAAGGCAATAAAAATAGCCGGAAAACGGTTAAACATCCCCCCAGCCGCTTTCAAACCGGATGCGGGTGGGTCGGGTCAAACCATGATTGACTCCGGTTCGGACCTGACTTATTTAGTGGATGAAGCGTATGAGAAGGTTAAAGAGGAGGTAGTGAGATTAGTGGGTGCGATGATGAAGAAAGGCTACGTATATGCCGCCGTAGCCGACATGTGTTTCGACGCCGGTGTGACGGTGGAGGTGGGCCGCAGGATTGGCGACATGTCGTTTGAGTTTGATAATGGAGTGGAGATTTTCGTGGGGAGAGGAGAAGGGGTTTTGACGGAGGTGGAAAAAGGAGTGAAGTGTGTGGGGATTGGACGGTCAGGAAGGCTTGGGATTGGAAGTAATATAATCGGTACCGTTCATCAACAGAATATGTGGGTGGAGTATGATTTGGCCAATAAGAGAGTAGGGTTTGGTGGAGCTGAGTGTAGCAGATTGAAGTGATGATGGACAGTAAAGATTTATACACGTGTGTGGGTTTTGGAATGTTTATATAATCATATTTGATATTGTGTTATTGTGTAAATGTGTGTATAGTTATTTCATTTCACATATATACTTCATATATAAATAAAACAAAATTTTTTCTATCCT

mRNA sequence

ATGTATATAATCCCCAACCCTCCACTTCACCAATCCCCCCCCCCAACACAATCAACAATGCTTCTAATTCTCTTCTCTCTCTCATTATTCACTCTCTCCTTCTCTCAATCCAATTCCCTCTCTCTCCCCTTCCCTCTTTCTCTCACTGAAAAACCCTCCAATATTACCCCATTATACTACTCTTCCCAGCTTTACGTCAAAAAGCCATCATCCCATGGCCCCTTCAAGCTTCCTTTCAAATACTCCTCCTCTGCCCTCGTCGTCTCTCTTCCGATCGGAACGCCGCCACAGCCCACTGACTTGGTTCTAGACACCGGCAGCCAACTCTCTTGGATTCAATGTCATGACAAAAAAGTTAAGAAAAGATTGCCCCCCTTGCCCAAGCCTAAAACCGCCTCCTTTGATCCTTCTCTCTCCTCTTCTTTTTCTCTCCTCCCTTGTAATCACCCCATCTGCAAACCCCGAATTCCAGATTTTACCCTTCCCACTTCTTGTGACCAAAATCGCCTCTGCCACTACTCCTACTTCTACGCTGACGGTACCTTGGCTGAGGGAAATCTCGTCAGAGAAAAATTTACCTTCTCTAATTCTCTTTCTACCCCTCCCGTCATCCTCGGTTGCGCTCAAGGCTCCACCGAAAACAGGGCCGCTTTCAAACCGGATGCGGGTGGGTCGGGTCAAACCATGATTGACTCCGGTTCGGACCTGACTTATTTAGTGGATGAAGCGTATGAGAAGGTTAAAGAGGAGGTAGTGAGATTAGTGGGTGCGATGATGAAGAAAGGCTACGTATATGCCGCCGTAGCCGACATGTGTTTCGACGCCGGTGTGACGGTGGAGGTGGGCCGCAGGATTGGCGACATGTCGTTTGAGTTTGATAATGGAGTGGAGATTTTCGTGGGGAGAGGAGAAGGGGTTTTGACGGAGGTGGAAAAAGGAGTGAAGTGTGTGGGGATTGGACGGTCAGGAAGGCTTGGGATTGGAAGTAATATAATCGGTACCGTTCATCAACAGAATATGTGGGTGGAGTATGATTTGGCCAATAAGAGAGTAGGGTTTGGTGGAGCTGAGTGTAGCAGATTGAAGTGA

Coding sequence (CDS)

ATGTATATAATCCCCAACCCTCCACTTCACCAATCCCCCCCCCCAACACAATCAACAATGCTTCTAATTCTCTTCTCTCTCTCATTATTCACTCTCTCCTTCTCTCAATCCAATTCCCTCTCTCTCCCCTTCCCTCTTTCTCTCACTGAAAAACCCTCCAATATTACCCCATTATACTACTCTTCCCAGCTTTACGTCAAAAAGCCATCATCCCATGGCCCCTTCAAGCTTCCTTTCAAATACTCCTCCTCTGCCCTCGTCGTCTCTCTTCCGATCGGAACGCCGCCACAGCCCACTGACTTGGTTCTAGACACCGGCAGCCAACTCTCTTGGATTCAATGTCATGACAAAAAAGTTAAGAAAAGATTGCCCCCCTTGCCCAAGCCTAAAACCGCCTCCTTTGATCCTTCTCTCTCCTCTTCTTTTTCTCTCCTCCCTTGTAATCACCCCATCTGCAAACCCCGAATTCCAGATTTTACCCTTCCCACTTCTTGTGACCAAAATCGCCTCTGCCACTACTCCTACTTCTACGCTGACGGTACCTTGGCTGAGGGAAATCTCGTCAGAGAAAAATTTACCTTCTCTAATTCTCTTTCTACCCCTCCCGTCATCCTCGGTTGCGCTCAAGGCTCCACCGAAAACAGGGCCGCTTTCAAACCGGATGCGGGTGGGTCGGGTCAAACCATGATTGACTCCGGTTCGGACCTGACTTATTTAGTGGATGAAGCGTATGAGAAGGTTAAAGAGGAGGTAGTGAGATTAGTGGGTGCGATGATGAAGAAAGGCTACGTATATGCCGCCGTAGCCGACATGTGTTTCGACGCCGGTGTGACGGTGGAGGTGGGCCGCAGGATTGGCGACATGTCGTTTGAGTTTGATAATGGAGTGGAGATTTTCGTGGGGAGAGGAGAAGGGGTTTTGACGGAGGTGGAAAAAGGAGTGAAGTGTGTGGGGATTGGACGGTCAGGAAGGCTTGGGATTGGAAGTAATATAATCGGTACCGTTCATCAACAGAATATGTGGGTGGAGTATGATTTGGCCAATAAGAGAGTAGGGTTTGGTGGAGCTGAGTGTAGCAGATTGAAGTGA

Protein sequence

MYIIPNPPLHQSPPPTQSTMLLILFSLSLFTLSFSQSNSLSLPFPLSLTEKPSNITPLYYSSQLYVKKPSSHGPFKLPFKYSSSALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKVKKRLPPLPKPKTASFDPSLSSSFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSNSLSTPPVILGCAQGSTENRAAFKPDAGGSGQTMIDSGSDLTYLVDEAYEKVKEEVVRLVGAMMKKGYVYAAVADMCFDAGVTVEVGRRIGDMSFEFDNGVEIFVGRGEGVLTEVEKGVKCVGIGRSGRLGIGSNIIGTVHQQNMWVEYDLANKRVGFGGAECSRLK*
Homology
[Close]
BLAST of Csa3G188340 vs. Swiss-Prot
Match: PCS1L_ARATH (Aspartic proteinase PCS1 OS=Arabidopsis thaliana GN=PCS1 PE=2 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 5.7e-27
Identity = 69/188 (36.70%), Postives = 102/188 (54.26%), Query Frame = 1 

Query: 20  MLLILFSLSLFTLSFSQSNSLSLPFPLSLTEKPSNITPLYYSSQLYVKKPSSHGPFKLPF 79
           +LL+L   +   +S S S+S S  F    +   S    L   +++    P+ H P     
Sbjct: 10  LLLVLSVRTYKCVSSSSSSSSSFSFSSFSSSSSSQTLVLPLKTRI---TPTDHRPTDKLH 69

Query: 80  KYSSSALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKVKKRLPPLPKPKTASFDPSLS 139
            + +  L V+L +GTPPQ   +V+DTGS+LSW++C+           P P   +FDP+ S
Sbjct: 70  FHHNVTLTVTLTVGTPPQNISMVIDTGSELSWLRCNRSSN-------PNPVN-NFDPTRS 129

Query: 140 SSFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSNSLS 199
           SS+S +PC+ P C+ R  DF +P SCD ++LCH +  YAD + +EGNL  E F F NS +
Sbjct: 130 SSYSPIPCSSPTCRTRTRDFLIPASCDSDKLCHATLSYADASSSEGNLAAEIFHFGNSTN 186

Query: 200 TPPVILGC 208
              +I GC
Sbjct: 190 DSNLIFGC 186


HSP 2 Score: 77.0 bits (188), Expect = 4.7e-13
Identity = 48/157 (30.57%), Postives = 75/157 (47.77%), Query Frame = 1 

Query: 215 RAAFKPDAGGSGQTMIDSGSDLTYLVDEAYEKVKEEVVRLVGAMMK----KGYVYAAVAD 274
           ++   PD  G+GQTM+DSG+  T+L+   Y  ++   +     ++       +V+    D
Sbjct: 288 KSVLVPDHTGAGQTMVDSGTQFTFLLGPVYTALRSHFLNRTNGILTVYEDPDFVFQGTMD 347

Query: 275 MCFDAG-VTVEVG--RRIGDMSFEFDNGVEIFVGRGEGVLTEV------EKGVKCVGIGR 334
           +C+    V +  G   R+  +S  F+ G EI V  G+ +L  V         V C   G 
Sbjct: 348 LCYRISPVRIRSGILHRLPTVSLVFE-GAEIAVS-GQPLLYRVPHLTVGNDSVYCFTFGN 407

Query: 335 SGRLGIGSNIIGTVHQQNMWVEYDLANKRVGFGGAEC 359
           S  +G+ + +IG  HQQNMW+E+DL   R+G    EC
Sbjct: 408 SDLMGMEAYVIGHHHQQNMWIEFDLQRSRIGLAPVEC 442

[Close]
BLAST of Csa3G188340 vs. Swiss-Prot
Match: AED1_ARATH (Aspartyl protease AED1 OS=Arabidopsis thaliana GN=AED1 PE=2 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 1.6e-13
Identity = 55/173 (31.79%), Postives = 77/173 (44.51%), Query Frame = 1 

Query: 70  SSHGPFKLPFKYSSSALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKVKKRLPPLPKP 129
           S+  P K      S   +V++ IGTP     LV DTGS L+W QC     +  L      
Sbjct: 116 STELPAKSGITLGSGNYIVTIGIGTPKHDLSLVFDTGSDLTWTQC-----EPCLGSCYSQ 175

Query: 130 KTASFDPSLSSSFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVR 189
           K   F+PS SS++  + C+ P+C+          SC  +  C YS  Y D +  +G L +
Sbjct: 176 KEPKFNPSSSSTYQNVSCSSPMCED-------AESCSASN-CVYSIVYGDKSFTQGFLAK 235

Query: 190 EKFTFSNSLSTPPVILGCAQGSTENRAAFKPDAG----GSGQTMIDSGSDLTY 239
           EKFT +NS     V  GC +    N+  F   AG    G G+  + + +  TY
Sbjct: 236 EKFTLTNSDVLEDVYFGCGE---NNQGLFDGVAGLLGLGPGKLSLPAQTTTTY 272

[Close]
BLAST of Csa3G188340 vs. Swiss-Prot
Match: NEP2_NEPGR (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis GN=nep2 PE=1 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 4.7e-13
Identity = 46/139 (33.09%), Postives = 60/139 (43.17%), Query Frame = 1 

Query: 71  SHGPFKLPFKYSSSALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKVKKRLPPLPKPK 130
           S    + P        ++++ IGTP      ++DTGS L W QC         P      
Sbjct: 81  SSSGIETPVYAGDGEYLMNVAIGTPDSSFSAIMDTGSDLIWTQCEPCTQCFSQP------ 140

Query: 131 TASFDPSLSSSFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVRE 190
           T  F+P  SSSFS LPC    C+       LP+    N  C Y+Y Y DG+  +G +  E
Sbjct: 141 TPIFNPQDSSSFSTLPCESQYCQD------LPSETCNNNECQYTYGYGDGSTTQGYMATE 200

Query: 191 KFTFSNSLSTPPVILGCAQ 210
            FTF  S S P +  GC +
Sbjct: 201 TFTFETS-SVPNIAFGCGE 206


HSP 2 Score: 60.8 bits (146), Expect = 3.4e-08
Identity = 47/146 (32.19%), Postives = 71/146 (48.63%), Query Frame = 1 

Query: 216 AAFKPDAGGSGQTMIDSGSDLTYLVDEAYEKVKEEVVRLVGAMMKKGYVYAAVADMCFDA 275
           + F+    G+G  +IDSG+ LTYL  +AY  V +     +   +      ++    CF  
Sbjct: 300 STFQLQDDGTGGMIIDSGTTLTYLPQDAYNAVAQAFTDQIN--LPTVDESSSGLSTCFQQ 359

Query: 276 ---GVTVEVGRRIGDMSFEFDNGVEIFVGRGEGVLTEVEKGVKCVGIGRSGRLGIGSNII 335
              G TV+V     ++S +FD GV + +G  + +L    +GV C+ +G S +LGI  +I 
Sbjct: 360 PSDGSTVQV----PEISMQFDGGV-LNLGE-QNILISPAEGVICLAMGSSSQLGI--SIF 419

Query: 336 GTVHQQNMWVEYDLANKRVGFGGAEC 359
           G + QQ   V YDL N  V F   +C
Sbjct: 420 GNIQQQETQVLYDLQNLAVSFVPTQC 435

[Close]
BLAST of Csa3G188340 vs. Swiss-Prot
Match: ASPG1_ARATH (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 2.8e-10
Identity = 44/132 (33.33%), Postives = 58/132 (43.94%), Query Frame = 1 

Query: 92  IGTPPQPTDLVLDTGSQLSWIQCHDKKVKKRLPPLPKPKTASFDPSLSSSFSLLPCNHPI 151
           +GTP +   LVLDTGS ++WIQC      +      +     F+P+ SS++  L C+ P 
Sbjct: 168 VGTPAKEMYLVLDTGSDVNWIQC------EPCADCYQQSDPVFNPTSSSTYKSLTCSAPQ 227

Query: 152 CKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSNSLSTPPVILGCAQGS 211
           C        L TS  ++  C Y   Y DG+   G L  +  TF NS     V LGC    
Sbjct: 228 CS------LLETSACRSNKCLYQVSYGDGSFTVGELATDTVTFGNSGKINNVALGCGH-- 284

Query: 212 TENRAAFKPDAG 224
            +N   F   AG
Sbjct: 288 -DNEGLFTGAAG 284


HSP 2 Score: 59.3 bits (142), Expect = 1.0e-07
Identity = 38/143 (26.57%), Postives = 65/143 (45.45%), Query Frame = 1 

Query: 216 AAFKPDAGGSGQTMIDSGSDLTYLVDEAYEKVKEEVVRLVGAMMKKGYVYAAVADMCFDA 275
           A F  DA GSG  ++D G+ +T L  +AY  +++  ++L    +KKG    ++ D C+D 
Sbjct: 364 AIFDVDASGSGGVILDCGTAVTRLQTQAYNSLRDAFLKLT-VNLKKGSSSISLFDTCYDF 423

Query: 276 GVTVEVGRRIGDMSFEFDNGVEIFVGRGEGVLTEVEKGVKCVGIGRSGRLGIGSNIIGTV 335
                V  ++  ++F F  G  + +     ++   + G  C     +       +IIG V
Sbjct: 424 SSLSTV--KVPTVAFHFTGGKSLDLPAKNYLIPVDDSGTFCFAFAPTSS---SLSIIGNV 483

Query: 336 HQQNMWVEYDLANKRVGFGGAEC 359
            QQ   + YDL+   +G  G +C
Sbjct: 484 QQQGTRITYDLSKNVIGLSGNKC 500

[Close]
BLAST of Csa3G188340 vs. Swiss-Prot
Match: NEP1_NEPGR (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis GN=nep1 PE=1 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 1.4e-09
Identity = 50/160 (31.25%), Postives = 73/160 (45.62%), Query Frame = 1 

Query: 206 GCAQGSTENRAAFKPDA------GGSGQTMIDSGSDLTYLVDEAYEKVKEEVVRLVGAMM 265
           G + GST  R    P A       G+G  +IDSG+ LTY V+ AY+ V++E +  +   +
Sbjct: 286 GLSVGST--RLPIDPSAFALNSNNGTGGIIIDSGTTLTYFVNNAYQSVRQEFISQINLPV 345

Query: 266 KKGYVYAAVADMCFDAGVTVEVGRRIGDMSFEFDNG-VEIFVGRGEGVLTEVEKGVKCVG 325
             G   ++  D+CF    +     +I      FD G +E+     E        G+ C+ 
Sbjct: 346 VNG--SSSGFDLCFQT-PSDPSNLQIPTFVMHFDGGDLEL---PSENYFISPSNGLICLA 405

Query: 326 IGRSGRLGIGSNIIGTVHQQNMWVEYDLANKRVGFGGAEC 359
           +G S +   G +I G + QQNM V YD  N  V F  A+C
Sbjct: 406 MGSSSQ---GMSIFGNIQQQNMLVVYDTGNSVVSFASAQC 434

[Close]
BLAST of Csa3G188340 vs. TrEMBL
Match: A0A0A0LBQ0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G188340 PE=3 SV=1)

HSP 1 Score: 737.6 bits (1903), Expect = 7.1e-210
Identity = 362/362 (100.00%), Postives = 362/362 (100.00%), Query Frame = 1 

Query: 1   MYIIPNPPLHQSPPPTQSTMLLILFSLSLFTLSFSQSNSLSLPFPLSLTEKPSNITPLYY 60
           MYIIPNPPLHQSPPPTQSTMLLILFSLSLFTLSFSQSNSLSLPFPLSLTEKPSNITPLYY
Sbjct: 1   MYIIPNPPLHQSPPPTQSTMLLILFSLSLFTLSFSQSNSLSLPFPLSLTEKPSNITPLYY 60

Query: 61  SSQLYVKKPSSHGPFKLPFKYSSSALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKVK 120
           SSQLYVKKPSSHGPFKLPFKYSSSALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKVK
Sbjct: 61  SSQLYVKKPSSHGPFKLPFKYSSSALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKVK 120

Query: 121 KRLPPLPKPKTASFDPSLSSSFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADG 180
           KRLPPLPKPKTASFDPSLSSSFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADG
Sbjct: 121 KRLPPLPKPKTASFDPSLSSSFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADG 180

Query: 181 TLAEGNLVREKFTFSNSLSTPPVILGCAQGSTENRAAFKPDAGGSGQTMIDSGSDLTYLV 240
           TLAEGNLVREKFTFSNSLSTPPVILGCAQGSTENRAAFKPDAGGSGQTMIDSGSDLTYLV
Sbjct: 181 TLAEGNLVREKFTFSNSLSTPPVILGCAQGSTENRAAFKPDAGGSGQTMIDSGSDLTYLV 240

Query: 241 DEAYEKVKEEVVRLVGAMMKKGYVYAAVADMCFDAGVTVEVGRRIGDMSFEFDNGVEIFV 300
           DEAYEKVKEEVVRLVGAMMKKGYVYAAVADMCFDAGVTVEVGRRIGDMSFEFDNGVEIFV
Sbjct: 241 DEAYEKVKEEVVRLVGAMMKKGYVYAAVADMCFDAGVTVEVGRRIGDMSFEFDNGVEIFV 300

Query: 301 GRGEGVLTEVEKGVKCVGIGRSGRLGIGSNIIGTVHQQNMWVEYDLANKRVGFGGAECSR 360
           GRGEGVLTEVEKGVKCVGIGRSGRLGIGSNIIGTVHQQNMWVEYDLANKRVGFGGAECSR
Sbjct: 301 GRGEGVLTEVEKGVKCVGIGRSGRLGIGSNIIGTVHQQNMWVEYDLANKRVGFGGAECSR 360

Query: 361 LK 363
           LK
Sbjct: 361 LK 362

[Close]
BLAST of Csa3G188340 vs. TrEMBL
Match: A0A0A0L6V5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G188350 PE=3 SV=1)

HSP 1 Score: 655.6 bits (1690), Expect = 3.5e-185
Identity = 326/343 (95.04%), Postives = 331/343 (96.50%), Query Frame = 1 

Query: 20  MLLILFSLSLFTLSFSQSNSLSLPFPLSLTEKPSNITPLYYSSQLYVKKPSSHGPFKLPF 79
           MLLILFSLSLFTLSFSQSNSLSLPFPLSL+EKPSN  P Y SSQLY K+PSS+G FKLPF
Sbjct: 1   MLLILFSLSLFTLSFSQSNSLSLPFPLSLSEKPSNTIPSY-SSQLYAKRPSSYGSFKLPF 60

Query: 80  KYSSSALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKVKKRLPPLPKPKTASFDPSLS 139
           KYSS+ALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKVKKRLPPLPKPKTASFDPSLS
Sbjct: 61  KYSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKVKKRLPPLPKPKTASFDPSLS 120

Query: 140 SSFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSNSLS 199
           SSFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFS SLS
Sbjct: 121 SSFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSKSLS 180

Query: 200 TPPVILGCAQGSTENRAAFKPDAGGSGQTMIDSGSDLTYLVDEAYEKVKEEVVRLVGAMM 259
           TPPVILGCAQ STENRAAFKPDAGGSGQTMIDSGSDLTYLVDEAYEKVKEEVVRLVGAMM
Sbjct: 181 TPPVILGCAQASTENRAAFKPDAGGSGQTMIDSGSDLTYLVDEAYEKVKEEVVRLVGAMM 240

Query: 260 KKGYVYAAVADMCFDAGVTVEVGRRIGDMSFEFDNGVEIFVGRGEGVLTEVEKGVKCVGI 319
           KKGYVYA VADMCFDAGVT EVGRRIG +SFEFDNGVEIFVGRGEGVLTEVEKGVKCVGI
Sbjct: 241 KKGYVYADVADMCFDAGVTAEVGRRIGGISFEFDNGVEIFVGRGEGVLTEVEKGVKCVGI 300

Query: 320 GRSGRLGIGSNIIGTVHQQNMWVEYDLANKRVGFGGAECSRLK 363
           GRS RLGIGSNIIGTVHQQNMWVEYDLANKRVGFGGAECSRLK
Sbjct: 301 GRSERLGIGSNIIGTVHQQNMWVEYDLANKRVGFGGAECSRLK 342

[Close]
BLAST of Csa3G188340 vs. TrEMBL
Match: A0A087GTV5_ARAAL (Uncharacterized protein OS=Arabis alpina GN=AALP_AA6G358100 PE=3 SV=1)

HSP 1 Score: 396.7 bits (1018), Expect = 3.0e-107
Identity = 214/360 (59.44%), Postives = 252/360 (70.00%), Query Frame = 1 

Query: 20  MLLILFSLSLFTL-SFSQSNSLSLPFPLSLTEKPSNITPLYYSSQLYVKKPSSHGPFKLP 79
           M L+L  + +F   S S S+S SL FPL  T  P+  +  + SS L    P S   F+  
Sbjct: 1   MSLVLTLVYIFLCNSLSLSSSYSLHFPLRRT--PTTNSSFFQSSLLSSPSPIS---FRSN 60

Query: 80  FKYSSSALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKVKKRLPPLPKPKTASFDPSL 139
           FKYS  AL++SLPIGTP QP +LVLDTGSQLSWIQC+ K          KP T SFDPS 
Sbjct: 61  FKYSV-ALIISLPIGTPSQPQELVLDTGSQLSWIQCNTKN--------KKPTTTSFDPSS 120

Query: 140 SSSFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSNSL 199
           SSSFS L C+HP+CKPRIPDFTLPTSCD N+LCHYSYFYADGT  EG LV+EKFTFSN+ 
Sbjct: 121 SSSFSNLLCSHPLCKPRIPDFTLPTSCDTNKLCHYSYFYADGTFTEGKLVKEKFTFSNNQ 180

Query: 200 STPPVILGCAQGSTENR----------------AAFKPDAGGSGQTMIDSGSDLTYLVDE 259
            TPP+ILGCA  S++N+                + F+PDAGGSGQTMIDSGS+ TYLVD 
Sbjct: 181 ITPPLILGCATESSDNKGILGIKIGQKRLNISSSVFRPDAGGSGQTMIDSGSEFTYLVDV 240

Query: 260 AYEKVKEEVVRLVGAMMKKGYVYAAVADMCFDAGVTVEVGRRIGDMSFEFDNGVEIFVGR 319
           AY+KVKEE+V+LVG  +KKGY+Y A ADMCFD    +E+GR IGD+ FEF +GVEI V +
Sbjct: 241 AYDKVKEEIVKLVGHRLKKGYMYGATADMCFDGNNPMEIGRLIGDLVFEFGSGVEIVVVK 300

Query: 320 GEGVLTEVEKGVKCVGIGRSGRLGIGSNIIGTVHQQNMWVEYDLANKRVGFGGAECSRLK 363
            E VL  V  G+ CVGIGRS  LG  SNIIG VHQQN+WVE+DL N+RVGF   ECS LK
Sbjct: 301 -ERVLVNVGGGIHCVGIGRSSMLGAASNIIGNVHQQNLWVEFDLTNRRVGFSKTECSGLK 345

[Close]
BLAST of Csa3G188340 vs. TrEMBL
Match: D7KTL4_ARALL (Predicted protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_676033 PE=3 SV=1)

HSP 1 Score: 287.3 bits (734), Expect = 2.5e-74
Identity = 172/346 (49.71%), Postives = 209/346 (60.40%), Query Frame = 1 

Query: 24  LFSLSLFTLSF-SQSNSLSLPFPL-SLTEKPSNITPLYYSSQLYVKKPSSHGP---FKLP 83
           LF    F  ++ S S+SLSL  PL SL    +  +  + +S L  K PS   P   F+  
Sbjct: 8   LFFFFFFLFNYVSLSSSLSLHLPLTSLPISSTTNSHRFTTSLLSRKNPSPSSPPYNFRSR 67

Query: 84  FKYSSSALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKVKKRLPPLPKPKTASFDPSL 143
           FKYS  AL++SLPIGTPPQ   +VLDTGSQLSWIQCH    +K+LPP  KPKT SFDPSL
Sbjct: 68  FKYSM-ALIISLPIGTPPQAQQMVLDTGSQLSWIQCH----RKKLPP--KPKT-SFDPSL 127

Query: 144 SSSFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSNSL 203
           SSSFS LPC+HP+CKPRIPDFTLPTSCD NRLCHYSYFYADGT AEGNLV+EK TFSN+ 
Sbjct: 128 SSSFSTLPCSHPLCKPRIPDFTLPTSCDSNRLCHYSYFYADGTFAEGNLVKEKITFSNTE 187

Query: 204 STPPVILGCAQGSTENRAAFKPDAGGSGQTMIDSGSDLTYLVDEAYEK---VKEEVVRLV 263
            TPP+ILGCA  S+++R     + G          +  +Y +     +          L 
Sbjct: 188 ITPPLILGCATESSDDRGILGMNRGRLSFVSQAKITKFSYCIPPKSNRPGFTPTGSFYLG 247

Query: 264 GAMMKKGYVYAAVADMCFDAGVTVEVGRRIGDMSFEFDNGVEIFVGRGEGVLTEVEKGVK 323
                KG+ Y ++                       F   VEI V + E VL  V  G+ 
Sbjct: 248 DNPNSKGFKYVSL---------------------LTFPERVEILVPK-ERVLVNVGDGIH 307

Query: 324 CVGIGRSGRLGIGSNIIGTVHQQNMWVEYDLANKRVGFGGAECSRL 362
           CVGIGRS  LG  SNIIG VHQQN+WVE+D+ N+RVGF  A+CSR+
Sbjct: 308 CVGIGRSSMLGAASNIIGNVHQQNLWVEFDVTNRRVGFARADCSRI 323

[Close]
BLAST of Csa3G188340 vs. TrEMBL
Match: Q9FGI3_ARATH (AT5g37540/mpa22_p_70 OS=Arabidopsis thaliana GN=At5g37540 PE=2 SV=1)

HSP 1 Score: 240.4 bits (612), Expect = 3.5e-60
Identity = 128/207 (61.84%), Postives = 152/207 (73.43%), Query Frame = 1 

Query: 21  LLILFSLSLFTLSFSQSNSLSLPFPL-SLTEKPSNITPLYYSSQLYVKKPS---SHGPFK 80
           LL +F    +++S S S+SLSL FPL SL   P+  +  + +S L  + PS   S   F+
Sbjct: 12  LLYIFFFFCYSVSLSWSSSLSLHFPLTSLRLTPTTNSSSFKTSLLSRRNPSPPSSPYTFR 71

Query: 81  LPFKYSSSALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKVKKRLPPLPKPKTASFDP 140
              KYS  AL++SLPIGTP Q  +LVLDTGSQLSWIQCH KK+KK LPP     T SFDP
Sbjct: 72  SNIKYSM-ALILSLPIGTPSQSQELVLDTGSQLSWIQCHPKKIKKPLPP----PTTSFDP 131

Query: 141 SLSSSFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSN 200
           SLSSSFS LPC+HP+CKPRIPDFTLPTSCD NRLCHYSYFYADGT AEGNLV+EKFTFSN
Sbjct: 132 SLSSSFSDLPCSHPLCKPRIPDFTLPTSCDSNRLCHYSYFYADGTFAEGNLVKEKFTFSN 191

Query: 201 SLSTPPVILGCAQGSTENRAAFKPDAG 224
           S +TPP+ILGCA+ ST+ +     + G
Sbjct: 192 SQTTPPLILGCAKESTDEKGILGMNLG 213

[Close]
BLAST of Csa3G188340 vs. TAIR10
Match: AT5G37540.1 (AT5G37540.1 Eukaryotic aspartyl protease family protein)

HSP 1 Score: 240.4 bits (612), Expect = 1.8e-63
Identity = 128/207 (61.84%), Postives = 150/207 (72.46%), Query Frame = 1 

Query: 21  LLILFSLSLFTLSFSQSNSLSLPFPL-SLTEKPSNITPLYYSSQLYVKKPS---SHGPFK 80
           LL +F    +++S S S+SLSL FPL SL   P+  +  + +S L  + PS   S   F+
Sbjct: 12  LLYIFFFFCYSVSLSWSSSLSLHFPLTSLRLTPTTNSSSFKTSLLSRRNPSPPSSPYTFR 71

Query: 81  LPFKYSSSALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKVKKRLPPLPKPKTASFDP 140
              KYS  AL++SLPIGTP Q  +LVLDTGSQLSWIQCH KK+KK LPP     T SFDP
Sbjct: 72  SNIKYSM-ALILSLPIGTPSQSQELVLDTGSQLSWIQCHPKKIKKPLPP----PTTSFDP 131

Query: 141 SLSSSFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSN 200
           SLSSSFS LPC+HP+CKPRIPDFTLPTSCD NRLCHYSYFYADGT AEGNLV+EKFTFSN
Sbjct: 132 SLSSSFSDLPCSHPLCKPRIPDFTLPTSCDSNRLCHYSYFYADGTFAEGNLVKEKFTFSN 191

Query: 201 SLSTPPVILGCAQGSTENRAAFKPDAG 224
           S +TPP+ILGCA+ ST+ +     + G
Sbjct: 192 SQTTPPLILGCAKESTDEKGILGMNLG 213


HSP 2 Score: 194.5 bits (493), Expect = 1.1e-49
Identity = 92/146 (63.01%), Postives = 112/146 (76.71%), Query Frame = 1 

Query: 216 AAFKPDAGGSGQTMIDSGSDLTYLVDEAYEKVKEEVVRLVGAMMKKGYVYAAVADMCFDA 275
           + F+PDAGGSGQTM+DSGS+ T+LVD AY+KVKEE+VRLVG+ +KKGYVY + ADMCFD 
Sbjct: 297 SVFRPDAGGSGQTMVDSGSEFTHLVDVAYDKVKEEIVRLVGSRLKKGYVYGSTADMCFDG 356

Query: 276 GVTVEVGRRIGDMSFEFDNGVEIFVGRGEGVLTEVEKGVKCVGIGRSGRLGIGSNIIGTV 335
             ++E+GR IGD+ FEF  GVEI V + + +L  V  G+ CVGIGRS  LG  SNIIG V
Sbjct: 357 NHSMEIGRLIGDLVFEFGRGVEILVEK-QSLLVNVGGGIHCVGIGRSSMLGAASNIIGNV 416

Query: 336 HQQNMWVEYDLANKRVGFGGAECSRL 362
           HQQN+WVE+D+ N+RVGF  AEC  L
Sbjct: 417 HQQNLWVEFDVTNRRVGFSKAECRLL 441

[Close]
BLAST of Csa3G188340 vs. TAIR10
Match: AT1G66180.1 (AT1G66180.1 Eukaryotic aspartyl protease family protein)

HSP 1 Score: 224.9 bits (572), Expect = 7.8e-59
Identity = 125/207 (60.39%), Postives = 142/207 (68.60%), Query Frame = 1 

Query: 23  ILFSLSLFTLSFSQSNSLSLPF---PLSLTEKPSNITPLYYSSQLYVKKPSSHGP---FK 82
           + F   L  +S S S SL LP    P+S T      T    +S L  K PS   P   F+
Sbjct: 8   LFFFFFLNYVSLSTSLSLHLPLTSLPISTTTNSHRFT----TSLLSRKNPSPSSPPYNFR 67

Query: 83  LPFKYSSSALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKVKKRLPPLPKPKTASFDP 142
             FKYS  AL++SLPIGTPPQ   +VLDTGSQLSWIQCH    +K+LPP  KPKT SFDP
Sbjct: 68  SRFKYSM-ALIISLPIGTPPQAQQMVLDTGSQLSWIQCH----RKKLPP--KPKT-SFDP 127

Query: 143 SLSSSFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSN 202
           SLSSSFS LPC+HP+CKPRIPDFTLPTSCD NRLCHYSYFYADGT AEGNLV+EK TFSN
Sbjct: 128 SLSSSFSTLPCSHPLCKPRIPDFTLPTSCDSNRLCHYSYFYADGTFAEGNLVKEKITFSN 187

Query: 203 SLSTPPVILGCAQGSTENRAAFKPDAG 224
           +  TPP+ILGCA  S+++R     + G
Sbjct: 188 TEITPPLILGCATESSDDRGILGMNRG 202


HSP 2 Score: 178.3 bits (451), Expect = 8.4e-45
Identity = 87/146 (59.59%), Postives = 106/146 (72.60%), Query Frame = 1 

Query: 216 AAFKPDAGGSGQTMIDSGSDLTYLVDEAYEKVKEEVVRLVGAMMKKGYVYAAVADMCFDA 275
           + F+PDAGGSGQTM+DSGS+ T+LVD AY+KV+ E++  VG  +KKGYVY   ADMCFD 
Sbjct: 286 SVFRPDAGGSGQTMVDSGSEFTHLVDAAYDKVRAEIMTRVGRRLKKGYVYGGTADMCFDG 345

Query: 276 GVTVEVGRRIGDMSFEFDNGVEIFVGRGEGVLTEVEKGVKCVGIGRSGRLGIGSNIIGTV 335
            V + + R IGD+ F F  GVEI V + E VL  V  G+ CVGIGRS  LG  SNIIG V
Sbjct: 346 NVAM-IPRLIGDLVFVFTRGVEILVPK-ERVLVNVGGGIHCVGIGRSSMLGAASNIIGNV 405

Query: 336 HQQNMWVEYDLANKRVGFGGAECSRL 362
           HQQN+WVE+D+ N+RVGF  A+CSR+
Sbjct: 406 HQQNLWVEFDVTNRRVGFAKADCSRV 429

[Close]
BLAST of Csa3G188340 vs. TAIR10
Match: AT5G02190.1 (AT5G02190.1 Eukaryotic aspartyl protease family protein)

HSP 1 Score: 123.2 bits (308), Expect = 3.2e-28
Identity = 69/188 (36.70%), Postives = 102/188 (54.26%), Query Frame = 1 

Query: 20  MLLILFSLSLFTLSFSQSNSLSLPFPLSLTEKPSNITPLYYSSQLYVKKPSSHGPFKLPF 79
           +LL+L   +   +S S S+S S  F    +   S    L   +++    P+ H P     
Sbjct: 10  LLLVLSVRTYKCVSSSSSSSSSFSFSSFSSSSSSQTLVLPLKTRI---TPTDHRPTDKLH 69

Query: 80  KYSSSALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKVKKRLPPLPKPKTASFDPSLS 139
            + +  L V+L +GTPPQ   +V+DTGS+LSW++C+           P P   +FDP+ S
Sbjct: 70  FHHNVTLTVTLTVGTPPQNISMVIDTGSELSWLRCNRSSN-------PNPVN-NFDPTRS 129

Query: 140 SSFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSNSLS 199
           SS+S +PC+ P C+ R  DF +P SCD ++LCH +  YAD + +EGNL  E F F NS +
Sbjct: 130 SSYSPIPCSSPTCRTRTRDFLIPASCDSDKLCHATLSYADASSSEGNLAAEIFHFGNSTN 186

Query: 200 TPPVILGC 208
              +I GC
Sbjct: 190 DSNLIFGC 186


HSP 2 Score: 77.0 bits (188), Expect = 2.6e-14
Identity = 48/157 (30.57%), Postives = 75/157 (47.77%), Query Frame = 1 

Query: 215 RAAFKPDAGGSGQTMIDSGSDLTYLVDEAYEKVKEEVVRLVGAMMK----KGYVYAAVAD 274
           ++   PD  G+GQTM+DSG+  T+L+   Y  ++   +     ++       +V+    D
Sbjct: 288 KSVLVPDHTGAGQTMVDSGTQFTFLLGPVYTALRSHFLNRTNGILTVYEDPDFVFQGTMD 347

Query: 275 MCFDAG-VTVEVG--RRIGDMSFEFDNGVEIFVGRGEGVLTEV------EKGVKCVGIGR 334
           +C+    V +  G   R+  +S  F+ G EI V  G+ +L  V         V C   G 
Sbjct: 348 LCYRISPVRIRSGILHRLPTVSLVFE-GAEIAVS-GQPLLYRVPHLTVGNDSVYCFTFGN 407

Query: 335 SGRLGIGSNIIGTVHQQNMWVEYDLANKRVGFGGAEC 359
           S  +G+ + +IG  HQQNMW+E+DL   R+G    EC
Sbjct: 408 SDLMGMEAYVIGHHHQQNMWIEFDLQRSRIGLAPVEC 442

[Close]
BLAST of Csa3G188340 vs. TAIR10
Match: AT2G39710.1 (AT2G39710.1 Eukaryotic aspartyl protease family protein)

HSP 1 Score: 112.1 bits (279), Expect = 7.4e-25
Identity = 76/210 (36.19%), Postives = 111/210 (52.86%), Query Frame = 1 

Query: 26  SLSLFTLSFSQSNSLSLPFPLSLTEKPSNITPLYYSSQLYVKKPSSHGPFKLPFKYSSSA 85
           SLSL + +F + + L L FPL+  +  S    L +S  L  +K       KL F+++ + 
Sbjct: 9   SLSL-SKNFLRISVLLLIFPLTFCKTSSTNQTLLFS--LKTQKLPQSSSDKLSFRHNVT- 68

Query: 86  LVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKVKKRLPPLPKPKTASFDPSLSSSFSLL 145
           L V+L +G PPQ   +VLDTGS+LSW+ C      K+ P L     + F+P  SS++S +
Sbjct: 69  LTVTLAVGDPPQNISMVLDTGSELSWLHC------KKSPNL----GSVFNPVSSSTYSPV 128

Query: 146 PCNHPICKPRIPDFTLPTSCD-QNRLCHYSYFYADGTLAEGNLVREKFTFSNSLSTPPVI 205
           PC+ PIC+ R  D  +P SCD +  LCH +  YAD T  EGNL  E F    S++ P  +
Sbjct: 129 PCSSPICRTRTRDLPIPASCDPKTHLCHVAISYADATSIEGNLAHETFVI-GSVTRPGTL 188

Query: 206 LGCAQGSTENRAAFKPDAGGSGQTMIDSGS 235
            GC      + +  + DA  +G   ++ GS
Sbjct: 189 FGCMDSGLSSNS--EEDAKSTGLMGMNRGS 201


HSP 2 Score: 87.8 bits (216), Expect = 1.5e-17
Identity = 51/151 (33.77%), Postives = 75/151 (49.67%), Query Frame = 1 

Query: 215 RAAFKPDAGGSGQTMIDSGSDLTYLVDEAYEKVKEEVVRLVGAMMK----KGYVYAAVAD 274
           ++ F PD  G+GQTM+DSG+  T+L+   Y  +K E +    ++++      +V+    D
Sbjct: 277 KSVFVPDHTGAGQTMVDSGTQFTFLMGPVYTALKNEFITQTKSVLRLVDDPDFVFQGTMD 336

Query: 275 MCFDAGVTVEVGRRIGDMSFEFDNGVEIFVG------RGEGVLTEVEKGVKCVGIGRSGR 334
           +C+  G T         M      G E+ V       R  G  +E ++ V C   G S  
Sbjct: 337 LCYKVGSTTRPNFSGLPMVSLMFRGAEMSVSGQKLLYRVNGAGSEGKEEVYCFTFGNSDL 396

Query: 335 LGIGSNIIGTVHQQNMWVEYDLANKRVGFGG 356
           LGI + +IG  HQQN+W+E+DLA  RVGF G
Sbjct: 397 LGIEAFVIGHHHQQNVWMEFDLAKSRVGFAG 427

[Close]
BLAST of Csa3G188340 vs. TAIR10
Match: AT5G10760.1 (AT5G10760.1 Eukaryotic aspartyl protease family protein)

HSP 1 Score: 78.6 bits (192), Expect = 9.0e-15
Identity = 55/173 (31.79%), Postives = 77/173 (44.51%), Query Frame = 1 

Query: 70  SSHGPFKLPFKYSSSALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKVKKRLPPLPKP 129
           S+  P K      S   +V++ IGTP     LV DTGS L+W QC     +  L      
Sbjct: 116 STELPAKSGITLGSGNYIVTIGIGTPKHDLSLVFDTGSDLTWTQC-----EPCLGSCYSQ 175

Query: 130 KTASFDPSLSSSFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVR 189
           K   F+PS SS++  + C+ P+C+          SC  +  C YS  Y D +  +G L +
Sbjct: 176 KEPKFNPSSSSTYQNVSCSSPMCED-------AESCSASN-CVYSIVYGDKSFTQGFLAK 235

Query: 190 EKFTFSNSLSTPPVILGCAQGSTENRAAFKPDAG----GSGQTMIDSGSDLTY 239
           EKFT +NS     V  GC +    N+  F   AG    G G+  + + +  TY
Sbjct: 236 EKFTLTNSDVLEDVYFGCGE---NNQGLFDGVAGLLGLGPGKLSLPAQTTTTY 272

[Close]
BLAST of Csa3G188340 vs. NCBI nr
Match: gi|700202328|gb|KGN57461.1| (hypothetical protein Csa_3G188340 [Cucumis sativus])

HSP 1 Score: 737.6 bits (1903), Expect = 1.0e-209
Identity = 362/362 (100.00%), Postives = 362/362 (100.00%), Query Frame = 1 

Query: 1   MYIIPNPPLHQSPPPTQSTMLLILFSLSLFTLSFSQSNSLSLPFPLSLTEKPSNITPLYY 60
           MYIIPNPPLHQSPPPTQSTMLLILFSLSLFTLSFSQSNSLSLPFPLSLTEKPSNITPLYY
Sbjct: 1   MYIIPNPPLHQSPPPTQSTMLLILFSLSLFTLSFSQSNSLSLPFPLSLTEKPSNITPLYY 60

Query: 61  SSQLYVKKPSSHGPFKLPFKYSSSALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKVK 120
           SSQLYVKKPSSHGPFKLPFKYSSSALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKVK
Sbjct: 61  SSQLYVKKPSSHGPFKLPFKYSSSALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKVK 120

Query: 121 KRLPPLPKPKTASFDPSLSSSFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADG 180
           KRLPPLPKPKTASFDPSLSSSFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADG
Sbjct: 121 KRLPPLPKPKTASFDPSLSSSFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADG 180

Query: 181 TLAEGNLVREKFTFSNSLSTPPVILGCAQGSTENRAAFKPDAGGSGQTMIDSGSDLTYLV 240
           TLAEGNLVREKFTFSNSLSTPPVILGCAQGSTENRAAFKPDAGGSGQTMIDSGSDLTYLV
Sbjct: 181 TLAEGNLVREKFTFSNSLSTPPVILGCAQGSTENRAAFKPDAGGSGQTMIDSGSDLTYLV 240

Query: 241 DEAYEKVKEEVVRLVGAMMKKGYVYAAVADMCFDAGVTVEVGRRIGDMSFEFDNGVEIFV 300
           DEAYEKVKEEVVRLVGAMMKKGYVYAAVADMCFDAGVTVEVGRRIGDMSFEFDNGVEIFV
Sbjct: 241 DEAYEKVKEEVVRLVGAMMKKGYVYAAVADMCFDAGVTVEVGRRIGDMSFEFDNGVEIFV 300

Query: 301 GRGEGVLTEVEKGVKCVGIGRSGRLGIGSNIIGTVHQQNMWVEYDLANKRVGFGGAECSR 360
           GRGEGVLTEVEKGVKCVGIGRSGRLGIGSNIIGTVHQQNMWVEYDLANKRVGFGGAECSR
Sbjct: 301 GRGEGVLTEVEKGVKCVGIGRSGRLGIGSNIIGTVHQQNMWVEYDLANKRVGFGGAECSR 360

Query: 361 LK 363
           LK
Sbjct: 361 LK 362

[Close]
BLAST of Csa3G188340 vs. NCBI nr
Match: gi|700202330|gb|KGN57463.1| (hypothetical protein Csa_3G188350 [Cucumis sativus])

HSP 1 Score: 655.6 bits (1690), Expect = 5.1e-185
Identity = 326/343 (95.04%), Postives = 331/343 (96.50%), Query Frame = 1 

Query: 20  MLLILFSLSLFTLSFSQSNSLSLPFPLSLTEKPSNITPLYYSSQLYVKKPSSHGPFKLPF 79
           MLLILFSLSLFTLSFSQSNSLSLPFPLSL+EKPSN  P Y SSQLY K+PSS+G FKLPF
Sbjct: 1   MLLILFSLSLFTLSFSQSNSLSLPFPLSLSEKPSNTIPSY-SSQLYAKRPSSYGSFKLPF 60

Query: 80  KYSSSALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKVKKRLPPLPKPKTASFDPSLS 139
           KYSS+ALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKVKKRLPPLPKPKTASFDPSLS
Sbjct: 61  KYSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKVKKRLPPLPKPKTASFDPSLS 120

Query: 140 SSFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSNSLS 199
           SSFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFS SLS
Sbjct: 121 SSFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSKSLS 180

Query: 200 TPPVILGCAQGSTENRAAFKPDAGGSGQTMIDSGSDLTYLVDEAYEKVKEEVVRLVGAMM 259
           TPPVILGCAQ STENRAAFKPDAGGSGQTMIDSGSDLTYLVDEAYEKVKEEVVRLVGAMM
Sbjct: 181 TPPVILGCAQASTENRAAFKPDAGGSGQTMIDSGSDLTYLVDEAYEKVKEEVVRLVGAMM 240

Query: 260 KKGYVYAAVADMCFDAGVTVEVGRRIGDMSFEFDNGVEIFVGRGEGVLTEVEKGVKCVGI 319
           KKGYVYA VADMCFDAGVT EVGRRIG +SFEFDNGVEIFVGRGEGVLTEVEKGVKCVGI
Sbjct: 241 KKGYVYADVADMCFDAGVTAEVGRRIGGISFEFDNGVEIFVGRGEGVLTEVEKGVKCVGI 300

Query: 320 GRSGRLGIGSNIIGTVHQQNMWVEYDLANKRVGFGGAECSRLK 363
           GRS RLGIGSNIIGTVHQQNMWVEYDLANKRVGFGGAECSRLK
Sbjct: 301 GRSERLGIGSNIIGTVHQQNMWVEYDLANKRVGFGGAECSRLK 342

[Close]
BLAST of Csa3G188340 vs. NCBI nr
Match: gi|778679910|ref|XP_011651212.1| (PREDICTED: aspartic proteinase PCS1-like [Cucumis sativus])

HSP 1 Score: 403.7 bits (1036), Expect = 3.5e-109
Identity = 196/196 (100.00%), Postives = 196/196 (100.00%), Query Frame = 1 

Query: 20  MLLILFSLSLFTLSFSQSNSLSLPFPLSLTEKPSNITPLYYSSQLYVKKPSSHGPFKLPF 79
           MLLILFSLSLFTLSFSQSNSLSLPFPLSLTEKPSNITPLYYSSQLYVKKPSSHGPFKLPF
Sbjct: 1   MLLILFSLSLFTLSFSQSNSLSLPFPLSLTEKPSNITPLYYSSQLYVKKPSSHGPFKLPF 60

Query: 80  KYSSSALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKVKKRLPPLPKPKTASFDPSLS 139
           KYSSSALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKVKKRLPPLPKPKTASFDPSLS
Sbjct: 61  KYSSSALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKVKKRLPPLPKPKTASFDPSLS 120

Query: 140 SSFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSNSLS 199
           SSFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSNSLS
Sbjct: 121 SSFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSNSLS 180

Query: 200 TPPVILGCAQGSTENR 216
           TPPVILGCAQGSTENR
Sbjct: 181 TPPVILGCAQGSTENR 196

[Close]
BLAST of Csa3G188340 vs. NCBI nr
Match: gi|778679910|ref|XP_011651212.1| (PREDICTED: aspartic proteinase PCS1-like [Cucumis sativus])

HSP 1 Score: 297.0 bits (759), Expect = 4.6e-77
Identity = 147/147 (100.00%), Postives = 147/147 (100.00%), Query Frame = 1 

Query: 216 AAFKPDAGGSGQTMIDSGSDLTYLVDEAYEKVKEEVVRLVGAMMKKGYVYAAVADMCFDA 275
           AAFKPDAGGSGQTMIDSGSDLTYLVDEAYEKVKEEVVRLVGAMMKKGYVYAAVADMCFDA
Sbjct: 285 AAFKPDAGGSGQTMIDSGSDLTYLVDEAYEKVKEEVVRLVGAMMKKGYVYAAVADMCFDA 344

Query: 276 GVTVEVGRRIGDMSFEFDNGVEIFVGRGEGVLTEVEKGVKCVGIGRSGRLGIGSNIIGTV 335
           GVTVEVGRRIGDMSFEFDNGVEIFVGRGEGVLTEVEKGVKCVGIGRSGRLGIGSNIIGTV
Sbjct: 345 GVTVEVGRRIGDMSFEFDNGVEIFVGRGEGVLTEVEKGVKCVGIGRSGRLGIGSNIIGTV 404

Query: 336 HQQNMWVEYDLANKRVGFGGAECSRLK 363
           HQQNMWVEYDLANKRVGFGGAECSRLK
Sbjct: 405 HQQNMWVEYDLANKRVGFGGAECSRLK 431


HSP 2 Score: 396.7 bits (1018), Expect = 4.2e-107
Identity = 214/360 (59.44%), Postives = 252/360 (70.00%), Query Frame = 1 

Query: 20  MLLILFSLSLFTL-SFSQSNSLSLPFPLSLTEKPSNITPLYYSSQLYVKKPSSHGPFKLP 79
           M L+L  + +F   S S S+S SL FPL  T  P+  +  + SS L    P S   F+  
Sbjct: 1   MSLVLTLVYIFLCNSLSLSSSYSLHFPLRRT--PTTNSSFFQSSLLSSPSPIS---FRSN 60

Query: 80  FKYSSSALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKVKKRLPPLPKPKTASFDPSL 139
           FKYS  AL++SLPIGTP QP +LVLDTGSQLSWIQC+ K          KP T SFDPS 
Sbjct: 61  FKYSV-ALIISLPIGTPSQPQELVLDTGSQLSWIQCNTKN--------KKPTTTSFDPSS 120

Query: 140 SSSFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSNSL 199
           SSSFS L C+HP+CKPRIPDFTLPTSCD N+LCHYSYFYADGT  EG LV+EKFTFSN+ 
Sbjct: 121 SSSFSNLLCSHPLCKPRIPDFTLPTSCDTNKLCHYSYFYADGTFTEGKLVKEKFTFSNNQ 180

Query: 200 STPPVILGCAQGSTENR----------------AAFKPDAGGSGQTMIDSGSDLTYLVDE 259
            TPP+ILGCA  S++N+                + F+PDAGGSGQTMIDSGS+ TYLVD 
Sbjct: 181 ITPPLILGCATESSDNKGILGIKIGQKRLNISSSVFRPDAGGSGQTMIDSGSEFTYLVDV 240

Query: 260 AYEKVKEEVVRLVGAMMKKGYVYAAVADMCFDAGVTVEVGRRIGDMSFEFDNGVEIFVGR 319
           AY+KVKEE+V+LVG  +KKGY+Y A ADMCFD    +E+GR IGD+ FEF +GVEI V +
Sbjct: 241 AYDKVKEEIVKLVGHRLKKGYMYGATADMCFDGNNPMEIGRLIGDLVFEFGSGVEIVVVK 300

Query: 320 GEGVLTEVEKGVKCVGIGRSGRLGIGSNIIGTVHQQNMWVEYDLANKRVGFGGAECSRLK 363
            E VL  V  G+ CVGIGRS  LG  SNIIG VHQQN+WVE+DL N+RVGF   ECS LK
Sbjct: 301 -ERVLVNVGGGIHCVGIGRSSMLGAASNIIGNVHQQNLWVEFDLTNRRVGFSKTECSGLK 345

[Close]
BLAST of Csa3G188340 vs. NCBI nr
Match: gi|778679913|ref|XP_004140731.2| (PREDICTED: aspartic proteinase PCS1-like [Cucumis sativus])

HSP 1 Score: 374.8 bits (961), Expect = 1.7e-100
Identity = 185/204 (90.69%), Postives = 190/204 (93.14%), Query Frame = 1 

Query: 20  MLLILFSLSLFTLSFSQSNSLSLPFPLSLTEKPSNITPLYYSSQLYVKKPSSHGPFKLPF 79
           MLLILFSLSLFTLSFSQSNSLSLPFPLSL+EKPSN  P Y SSQLY K+PSS+G FKLPF
Sbjct: 1   MLLILFSLSLFTLSFSQSNSLSLPFPLSLSEKPSNTIPSY-SSQLYAKRPSSYGSFKLPF 60

Query: 80  KYSSSALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKVKKRLPPLPKPKTASFDPSLS 139
           KYSS+ALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKVKKRLPPLPKPKTASFDPSLS
Sbjct: 61  KYSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKVKKRLPPLPKPKTASFDPSLS 120

Query: 140 SSFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSNSLS 199
           SSFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFS SLS
Sbjct: 121 SSFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSKSLS 180

Query: 200 TPPVILGCAQGSTENRAAFKPDAG 224
           TPPVILGCAQ STENR     + G
Sbjct: 181 TPPVILGCAQASTENRGILGMNRG 203

The following BLAST results are available for this feature:
BLAST of Csa3G188340 vs. Swiss-Prot
Match NameE-valueIdentityDescription
PCS1L_ARATH5.7e-2736.70Aspartic proteinase PCS1 OS=Arabidopsis thaliana GN=PCS1 PE=2 SV=1[more]
AED1_ARATH1.6e-1331.79Aspartyl protease AED1 OS=Arabidopsis thaliana GN=AED1 PE=2 SV=1[more]
NEP2_NEPGR4.7e-1333.09Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis GN=nep2 PE=1 SV=1[more]
ASPG1_ARATH2.8e-1033.33Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana GN=ASPG1 PE=1 ... [more]
NEP1_NEPGR1.4e-0931.25Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis GN=nep1 PE=1 SV=1[more]
BLAST of Csa3G188340 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LBQ0_CUCSA7.1e-210100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_3G188340 PE=3 SV=1[more]
A0A0A0L6V5_CUCSA3.5e-18595.04Uncharacterized protein OS=Cucumis sativus GN=Csa_3G188350 PE=3 SV=1[more]
A0A087GTV5_ARAAL3.0e-10759.44Uncharacterized protein OS=Arabis alpina GN=AALP_AA6G358100 PE=3 SV=1[more]
D7KTL4_ARALL2.5e-7449.71Predicted protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_676033 PE=3 ... [more]
Q9FGI3_ARATH3.5e-6061.84AT5g37540/mpa22_p_70 OS=Arabidopsis thaliana GN=At5g37540 PE=2 SV=1[more]
BLAST of Csa3G188340 vs. TAIR10
Match NameE-valueIdentityDescription
AT5G37540.11.8e-6361.84 Eukaryotic aspartyl protease family protein[more]
AT1G66180.17.8e-5960.39 Eukaryotic aspartyl protease family protein[more]
AT5G02190.13.2e-2836.70 Eukaryotic aspartyl protease family protein[more]
AT2G39710.17.4e-2536.19 Eukaryotic aspartyl protease family protein[more]
AT5G10760.19.0e-1531.79 Eukaryotic aspartyl protease family protein[more]
BLAST of Csa3G188340 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|700202328|gb|KGN57461.1|1.0e-209100.00hypothetical protein Csa_3G188340 [Cucumis sativus][more]
gi|700202330|gb|KGN57463.1|5.1e-18595.04hypothetical protein Csa_3G188350 [Cucumis sativus][more]
gi|778679910|ref|XP_011651212.1|3.5e-109100.00PREDICTED: aspartic proteinase PCS1-like [Cucumis sativus][more]
gi|778679910|ref|XP_011651212.1|4.6e-77100.00PREDICTED: aspartic proteinase PCS1-like [Cucumis sativus][more]
gi|778679913|ref|XP_004140731.2|1.7e-10090.69PREDICTED: aspartic proteinase PCS1-like [Cucumis sativus][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001461Aspartic_peptidase_A1
IPR001969Aspartic_peptidase_AS
IPR021109Peptidase_aspartic_dom_sf
Vocabulary: Molecular Function
TermDefinition
GO:0004190aspartic-type endopeptidase activity
Vocabulary: Biological Process
TermDefinition
GO:0006508proteolysis
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
biological_process GO:0015992 proton transport
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
biological_process GO:0006744 ubiquinone biosynthetic process
biological_process GO:0006814 sodium ion transport
biological_process GO:0051788 response to misfolded protein
biological_process GO:0006979 response to oxidative stress
biological_process GO:0080129 proteasome core complex assembly
biological_process GO:0009853 photorespiration
biological_process GO:0006120 mitochondrial electron transport, NADH to ubiquinone
biological_process GO:0009630 gravitropism
cellular_component GO:0005575 cellular_component
cellular_component GO:0009507 chloroplast
cellular_component GO:0005747 mitochondrial respiratory chain complex I
molecular_function GO:0016740 transferase activity
molecular_function GO:0004190 aspartic-type endopeptidase activity
molecular_function GO:0051537 2 iron, 2 sulfur cluster binding
molecular_function GO:0051539 4 iron, 4 sulfur cluster binding
molecular_function GO:0009055 electron carrier activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0008137 NADH dehydrogenase (ubiquinone) activity
Unigene
This gene is associated with the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
CU119857cucumber EST collection version 3.0transcribed_cluster
CU158437cucumber EST collection version 3.0transcribed_cluster
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csa3G188340.1Csa3G188340.1mRNA


The following transcribed_cluster feature(s) are associated with this gene:

Feature NameUnique NameType
CU119857CU119857transcribed_cluster
CU158437CU158437transcribed_cluster


InterPro
Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001461Aspartic peptidase A1 familyPRINTSPR00792PEPSINcoord: 92..112
score: 2.0E-5coord: 228..239
score: 2.0E-5coord: 330..345
score: 2.
IPR001461Aspartic peptidase A1 familyPANTHERPTHR13683ASPARTYL PROTEASEScoord: 15..361
score: 2.2E
IPR001969Aspartic peptidase, active sitePROSITEPS00141ASP_PROTEASEcoord: 101..112
scor
IPR021109Aspartic peptidase domainGENE3DG3DSA:2.40.70.10coord: 218..360
score: 7.1E-25coord: 84..215
score: 1.3
IPR021109Aspartic peptidase domainunknownSSF50630Acid proteasescoord: 83..360
score: 7.76
NoneNo IPR availablePANTHERPTHR13683:SF327ASPARTYL PROTEASE FAMILY PROTEINcoord: 15..361
score: 2.2E