Csa1G132700 (gene) Cucumber (Chinese Long) v2 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Csa1G132700
Type	gene
Organism	Cucumis. sativus (Cucumber (Chinese Long) v2)
Description	Pheophorbide A oxygenase, putative; contains IPR013626 (Pheophorbide a oxygenase)
Location	Chr1 : 9455243 .. 9456053 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAATAGTAAGAACTAACTAGAGGTTCTTTAGTTTTGTGAAATTACGAGACATCTCAAAACTTGACTCTTGGAGCTTTTCTCAAAAAAAAAAAAAAACCAGTTACAATACAAGTCCTCTAGCATAAAATTAGCAACACCCTACTGAATTTATTGGCAACTGTTTCAACATTCTATTTCCTTTTGTTTAAGTGCTAAATATTATGAATCCCTTATACACAAATTCCTATAGGTTTTAACTTTATTGTAAAGTTTTCTTCGGAGTGGAGATTGACACAAAGCTTCCTTTACTTGGCGATAAAAAATGGGTAATGTGGATTTCCTCCTTCAATGTGCTAGTGGGTCCTGGAAAAATTCGTTTTATTGTATGTACTGCTCGAAACTTCTTCCAGTTTTCAATGGCCGGTCCTGTTTGATGGCAGGCAAGCAGTGCGGTGTTAATTAGATCTGTTTCTTATTTTTATGATATACTATCATCTCCACCGTTATTGGTCATTCAACATGCATTATATGTGATATTACAGTGTTTGACTTCCTAAGTTTGCTTTTTCTATTAGGTAGTTCCTCGATGGCACAAGCATTGGACTTGGAATAAGGTATATGATGGAGACATGATTGTTCTTTAGGGTCAAGAGAAGATATTTTTATCAATGGAAGGTTCGACAAATGTAAAGAATACACTAAAATAACGTTCACACCATAGCATTCAGAAATTGGCTGAATCGGCATGGAAAGGGCCAAAACCGAATAGTTCAGAGCTAGCAGCCACTAGCGCCTGTCATCAACAGTTCTATCTAGAACTCAGGTACTGA

mRNA sequence

ATGAATATTTTCTTCGGAGTGGAGATTGACACAAAGCTTCCTTTACTTGGCGATAAAAAATGGGTAATGTGGATTTCCTCCTTCAATGTGCTAGTGGGTCCTGGAAAAATTCGTTTTATTGTATGTACTGCTCGAAACTTCTTCCAGTTTTCAATGGCCGGTCCTGTAGTTCCTCGATGGCACAAGCATTGGACTTGGAATAAGAAATTGGCTGAATCGGCATGGAAAGGGCCAAAACCGAATAGTTCAGAGCTAGCAGCCACTAGCGCCTGTCATCAACAGTTCTATCTAGAACTCAGGTACTGA

Coding sequence (CDS)

ATGAATATTTTCTTCGGAGTGGAGATTGACACAAAGCTTCCTTTACTTGGCGATAAAAAATGGGTAATGTGGATTTCCTCCTTCAATGTGCTAGTGGGTCCTGGAAAAATTCGTTTTATTGTATGTACTGCTCGAAACTTCTTCCAGTTTTCAATGGCCGGTCCTGTAGTTCCTCGATGGCACAAGCATTGGACTTGGAATAAGAAATTGGCTGAATCGGCATGGAAAGGGCCAAAACCGAATAGTTCAGAGCTAGCAGCCACTAGCGCCTGTCATCAACAGTTCTATCTAGAACTCAGGTACTGA

Protein sequence

MNIFFGVEIDTKLPLLGDKKWVMWISSFNVLVGPGKIRFIVCTARNFFQFSMAGPVVPRWHKHWTWNKKLAESAWKGPKPNSSELAATSACHQQFYLELRY*

Homology

[Close]

BLAST of Csa1G132700 vs. Swiss-Prot
Match: PAO_ARATH (Pheophorbide a oxygenase, chloroplastic OS=Arabidopsis thaliana GN=PAO PE=1 SV=1)

HSP 1 Score: 94.7 bits (234), Expect = 6.1e-19
Identity = 40/65 (61.54%), Postives = 51/65 (78.46%), Query Frame = 1


Query: 7   VEIDTKLPLLGDKKWVMWISSFNVLVGPGKIRFIVCTARNFFQFSMAGP----VVPRWHK 66
           +E+D KLP++G++KWV+WI SFN+ + PGK R IVC+ARNFFQFS+ GP    VVPRW++
Sbjct: 306 IELDAKLPIVGNQKWVIWICSFNIPMAPGKTRSIVCSARNFFQFSVPGPAWWQVVPRWYE 365

Query: 67  HWTWN 68
           HWT N
Sbjct: 366 HWTSN 370

[Close]

BLAST of Csa1G132700 vs. TrEMBL
Match: A0A0A0LSG8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G132700 PE=4 SV=1)

HSP 1 Score: 220.7 bits (561), Expect = 8.1e-55
Identity = 101/101 (100.00%), Postives = 101/101 (100.00%), Query Frame = 1


Query: 1   MNIFFGVEIDTKLPLLGDKKWVMWISSFNVLVGPGKIRFIVCTARNFFQFSMAGPVVPRW 60
           MNIFFGVEIDTKLPLLGDKKWVMWISSFNVLVGPGKIRFIVCTARNFFQFSMAGPVVPRW
Sbjct: 1   MNIFFGVEIDTKLPLLGDKKWVMWISSFNVLVGPGKIRFIVCTARNFFQFSMAGPVVPRW 60

Query: 61  HKHWTWNKKLAESAWKGPKPNSSELAATSACHQQFYLELRY 102
           HKHWTWNKKLAESAWKGPKPNSSELAATSACHQQFYLELRY
Sbjct: 61  HKHWTWNKKLAESAWKGPKPNSSELAATSACHQQFYLELRY 101

[Close]

BLAST of Csa1G132700 vs. TrEMBL
Match: A0A0A0LSG3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G132160 PE=4 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 4.5e-21
Identity = 50/66 (75.76%), Postives = 56/66 (84.85%), Query Frame = 1


Query: 7   VEIDTKLPLLGDKKWVMWISSFNVLVGPGKIRFIVCTARNFFQFSMAGP----VVPRWHK 66
           VEID+KLPLLGD+KWV+WI SFNV +GPGK R IVC+ARNFFQFSM GP    VVPRWH+
Sbjct: 325 VEIDSKLPLLGDQKWVIWICSFNVPMGPGKTRSIVCSARNFFQFSMPGPAWWQVVPRWHE 384

Query: 67  HWTWNK 69
           HWT NK
Sbjct: 385 HWTSNK 390

[Close]

BLAST of Csa1G132700 vs. TrEMBL
Match: C6L809_CUCSA (Putative pheide a oxygenase (Fragment) OS=Cucumis sativus GN=CsPAO PE=2 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 4.5e-21
Identity = 50/66 (75.76%), Postives = 56/66 (84.85%), Query Frame = 1


Query: 7   VEIDTKLPLLGDKKWVMWISSFNVLVGPGKIRFIVCTARNFFQFSMAGP----VVPRWHK 66
           VEID+KLPLLGD+KWV+WI SFNV +GPGK R IVC+ARNFFQFSM GP    VVPRWH+
Sbjct: 91  VEIDSKLPLLGDQKWVIWICSFNVPMGPGKTRSIVCSARNFFQFSMPGPAWWQVVPRWHE 150

Query: 67  HWTWNK 69
           HWT NK
Sbjct: 151 HWTSNK 156

[Close]

BLAST of Csa1G132700 vs. TrEMBL
Match: M5WRF3_PRUPE (Uncharacterized protein (Fragment) OS=Prunus persica GN=PRUPE_ppa019738mg PE=4 SV=1)

HSP 1 Score: 105.9 bits (263), Expect = 2.9e-20
Identity = 47/66 (71.21%), Postives = 55/66 (83.33%), Query Frame = 1


Query: 7   VEIDTKLPLLGDKKWVMWISSFNVLVGPGKIRFIVCTARNFFQFSMAGP----VVPRWHK 66
           +EIDTKLPL+GD+KW++WI SFNV + PGK R IVC+ARNFFQFSM GP    VVPRWH+
Sbjct: 292 IEIDTKLPLVGDQKWIIWICSFNVPMAPGKTRSIVCSARNFFQFSMPGPAWWQVVPRWHE 351

Query: 67  HWTWNK 69
           HWT NK
Sbjct: 352 HWTSNK 357

[Close]

BLAST of Csa1G132700 vs. TrEMBL
Match: U5GK95_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0004s22680g PE=4 SV=1)

HSP 1 Score: 104.0 bits (258), Expect = 1.1e-19
Identity = 45/66 (68.18%), Postives = 55/66 (83.33%), Query Frame = 1


Query: 7   VEIDTKLPLLGDKKWVMWISSFNVLVGPGKIRFIVCTARNFFQFSMAGP----VVPRWHK 66
           VEIDTKLP++GD+KW++WI SFNV + PGK R IVC+ARNFFQF+M GP    V+PRWH+
Sbjct: 306 VEIDTKLPIVGDQKWIIWICSFNVPMAPGKTRSIVCSARNFFQFTMPGPAWWQVIPRWHE 365

Query: 67  HWTWNK 69
           HWT NK
Sbjct: 366 HWTSNK 371

[Close]

BLAST of Csa1G132700 vs. TAIR10
Match: AT3G44880.1 (AT3G44880.1 Pheophorbide a oxygenase family protein with Rieske [2Fe-2S] domain)

HSP 1 Score: 94.7 bits (234), Expect = 3.4e-20
Identity = 40/65 (61.54%), Postives = 51/65 (78.46%), Query Frame = 1


Query: 7   VEIDTKLPLLGDKKWVMWISSFNVLVGPGKIRFIVCTARNFFQFSMAGP----VVPRWHK 66
           +E+D KLP++G++KWV+WI SFN+ + PGK R IVC+ARNFFQFS+ GP    VVPRW++
Sbjct: 306 IELDAKLPIVGNQKWVIWICSFNIPMAPGKTRSIVCSARNFFQFSVPGPAWWQVVPRWYE 365

Query: 67  HWTWN 68
           HWT N
Sbjct: 366 HWTSN 370

[Close]

BLAST of Csa1G132700 vs. NCBI nr
Match: gi|700209764|gb|KGN64860.1| (hypothetical protein Csa_1G132700 [Cucumis sativus])

HSP 1 Score: 220.7 bits (561), Expect = 1.2e-54
Identity = 101/101 (100.00%), Postives = 101/101 (100.00%), Query Frame = 1


Query: 1   MNIFFGVEIDTKLPLLGDKKWVMWISSFNVLVGPGKIRFIVCTARNFFQFSMAGPVVPRW 60
           MNIFFGVEIDTKLPLLGDKKWVMWISSFNVLVGPGKIRFIVCTARNFFQFSMAGPVVPRW
Sbjct: 1   MNIFFGVEIDTKLPLLGDKKWVMWISSFNVLVGPGKIRFIVCTARNFFQFSMAGPVVPRW 60

Query: 61  HKHWTWNKKLAESAWKGPKPNSSELAATSACHQQFYLELRY 102
           HKHWTWNKKLAESAWKGPKPNSSELAATSACHQQFYLELRY
Sbjct: 61  HKHWTWNKKLAESAWKGPKPNSSELAATSACHQQFYLELRY 101

[Close]

BLAST of Csa1G132700 vs. NCBI nr
Match: gi|659071896|ref|XP_008462467.1| (PREDICTED: pheophorbide a oxygenase, chloroplastic [Cucumis melo])

HSP 1 Score: 110.2 bits (274), Expect = 2.2e-21
Identity = 51/66 (77.27%), Postives = 56/66 (84.85%), Query Frame = 1


Query: 7   VEIDTKLPLLGDKKWVMWISSFNVLVGPGKIRFIVCTARNFFQFSMAGP----VVPRWHK 66
           VEIDTKLPLLGD+KWV+WI SFNV +GPGK R IVC+ARNFFQFSM GP    VVPRWH+
Sbjct: 318 VEIDTKLPLLGDQKWVIWICSFNVPMGPGKTRSIVCSARNFFQFSMPGPAWWQVVPRWHE 377

Query: 67  HWTWNK 69
           HWT NK
Sbjct: 378 HWTSNK 383

[Close]

BLAST of Csa1G132700 vs. NCBI nr
Match: gi|778659272|ref|XP_011654100.1| (PREDICTED: LOW QUALITY PROTEIN: pheophorbide a oxygenase, chloroplastic [Cucumis sativus])

HSP 1 Score: 108.6 bits (270), Expect = 6.5e-21
Identity = 50/66 (75.76%), Postives = 56/66 (84.85%), Query Frame = 1


Query: 7   VEIDTKLPLLGDKKWVMWISSFNVLVGPGKIRFIVCTARNFFQFSMAGP----VVPRWHK 66
           VEID+KLPLLGD+KWV+WI SFNV +GPGK R IVC+ARNFFQFSM GP    VVPRWH+
Sbjct: 319 VEIDSKLPLLGDQKWVIWICSFNVPMGPGKTRSIVCSARNFFQFSMPGPAWWQVVPRWHE 378

Query: 67  HWTWNK 69
           HWT NK
Sbjct: 379 HWTSNK 384

[Close]

BLAST of Csa1G132700 vs. NCBI nr
Match: gi|253787593|dbj|BAH84856.1| (putative pheide a oxygenase, partial [Cucumis sativus])

HSP 1 Score: 108.6 bits (270), Expect = 6.5e-21
Identity = 50/66 (75.76%), Postives = 56/66 (84.85%), Query Frame = 1


Query: 7   VEIDTKLPLLGDKKWVMWISSFNVLVGPGKIRFIVCTARNFFQFSMAGP----VVPRWHK 66
           VEID+KLPLLGD+KWV+WI SFNV +GPGK R IVC+ARNFFQFSM GP    VVPRWH+
Sbjct: 91  VEIDSKLPLLGDQKWVIWICSFNVPMGPGKTRSIVCSARNFFQFSMPGPAWWQVVPRWHE 150

Query: 67  HWTWNK 69
           HWT NK
Sbjct: 151 HWTSNK 156

[Close]

BLAST of Csa1G132700 vs. NCBI nr
Match: gi|700209759|gb|KGN64855.1| (hypothetical protein Csa_1G132160 [Cucumis sativus])

HSP 1 Score: 108.6 bits (270), Expect = 6.5e-21
Identity = 50/66 (75.76%), Postives = 56/66 (84.85%), Query Frame = 1


Query: 7   VEIDTKLPLLGDKKWVMWISSFNVLVGPGKIRFIVCTARNFFQFSMAGP----VVPRWHK 66
           VEID+KLPLLGD+KWV+WI SFNV +GPGK R IVC+ARNFFQFSM GP    VVPRWH+
Sbjct: 325 VEIDSKLPLLGDQKWVIWICSFNVPMGPGKTRSIVCSARNFFQFSMPGPAWWQVVPRWHE 384

Query: 67  HWTWNK 69
           HWT NK
Sbjct: 385 HWTSNK 390

The following BLAST results are available for this feature:

BLAST of Csa1G132700 vs. Swiss-Prot


Match Name	E-value	Identity	Description
PAO_ARATH	6.1e-19	61.54	Pheophorbide a oxygenase, chloroplastic OS=Arabidopsis thaliana GN=PAO PE=1 SV=1	[more]

BLAST of Csa1G132700 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LSG8_CUCSA	8.1e-55	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G132700 PE=4 SV=1	[more]
A0A0A0LSG3_CUCSA	4.5e-21	75.76	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G132160 PE=4 SV=1	[more]
C6L809_CUCSA	4.5e-21	75.76	Putative pheide a oxygenase (Fragment) OS=Cucumis sativus GN=CsPAO PE=2 SV=1	[more]
M5WRF3_PRUPE	2.9e-20	71.21	Uncharacterized protein (Fragment) OS=Prunus persica GN=PRUPE_ppa019738mg PE=4 S...	[more]
U5GK95_POPTR	1.1e-19	68.18	Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0004s22680g PE=4 SV=1	[more]

BLAST of Csa1G132700 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G44880.1	3.4e-20	61.54	Pheophorbide a oxygenase family protein with Rieske [2Fe-2S] domain	[more]

BLAST of Csa1G132700 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700209764\|gb\|KGN64860.1\|	1.2e-54	100.00	hypothetical protein Csa_1G132700 [Cucumis sativus]	[more]
gi\|659071896\|ref\|XP_008462467.1\|	2.2e-21	77.27	PREDICTED: pheophorbide a oxygenase, chloroplastic [Cucumis melo]	[more]
gi\|778659272\|ref\|XP_011654100.1\|	6.5e-21	75.76	PREDICTED: LOW QUALITY PROTEIN: pheophorbide a oxygenase, chloroplastic [Cucumis...	[more]
gi\|253787593\|dbj\|BAH84856.1\|	6.5e-21	75.76	putative pheide a oxygenase, partial [Cucumis sativus]	[more]
gi\|700209759\|gb\|KGN64855.1\|	6.5e-21	75.76	hypothetical protein Csa_1G132160 [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR013626	PaO

Vocabulary: **Molecular Function**
Term	Definition
GO:0010277	chlorophyllide a oxygenase [overall] activity

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	oxidation-reduction process

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008219	cell death
biological_process	GO:0015996	chlorophyll catabolic process
biological_process	GO:0009816	defense response to bacterium, incompatible interaction
biological_process	GO:0009908	flower development
biological_process	GO:0010154	fruit development
biological_process	GO:0055114	oxidation-reduction process
cellular_component	GO:0009941	chloroplast envelope
cellular_component	GO:0009534	chloroplast thylakoid
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0051537	2 iron, 2 sulfur cluster binding
molecular_function	GO:0010277	chlorophyllide a oxygenase [overall] activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0032441	pheophorbide a oxygenase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Csa1G132700.1	Csa1G132700.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013626	Pheophorbide a oxygenase	PFAM	PF08417	PaO	coord: 20..68 score: 9.
None	No IPR available	PANTHER	PTHR21266	IRON-SULFUR DOMAIN CONTAINING PROTEIN	coord: 7..68 score: 5.5
None	No IPR available	PANTHER	PTHR21266:SF24	PHEOPHORBIDE A OXYGENASE, CHLOROPLASTIC	coord: 7..68 score: 5.5

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Csa1G132700	CsGy1G013970	Cucumber (Gy14) v2	cgybcuB001

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Csa1G132700	Watermelon (97103) v2	cuwmbB002
Csa1G132700	Wax gourd	cuwgoB049
Csa1G132700	Cucumber (Gy14) v1	cgycuB305
Csa1G132700	Cucurbita maxima (Rimu)	cmacuB163
Csa1G132700	Cucurbita maxima (Rimu)	cmacuB203
Csa1G132700	Cucurbita moschata (Rifu)	cmocuB148
Csa1G132700	Cucurbita moschata (Rifu)	cmocuB188
Csa1G132700	Melon (DHL92) v3.5.1	cumeB015
Csa1G132700	Watermelon (Charleston Gray)	cuwcgB014
Csa1G132700	Cucurbita pepo (Zucchini)	cpecuB516
Csa1G132700	Cucurbita pepo (Zucchini)	cpecuB805
Csa1G132700	Bottle gourd (USVL1VR-Ls)	culsiB044
Csa1G132700	Melon (DHL92) v3.6.1	cumedB010
Csa1G132700	Silver-seed gourd	carcuB0185