Csa1G025800 (gene) Cucumber (Chinese Long) v2 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Csa1G025800
Type	gene
Organism	Cucumis. sativus (Cucumber (Chinese Long) v2)
Description	Cycloartenol synthase; contains IPR008930 (Terpenoid cyclases/protein prenyltransferase alpha-alpha toroid)
Location	Chr1 : 2866388 .. 2868258 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTGGAAGCTCAAAGTTTCGGAAGGATGGGAGACAAGTGAGAACGATCACGTAGGAAGACAATATTGGAAATTCGACACAAATCTCACACCTTCTGAAGAAGAAAAAGCTCAAATACAAAAGTTTTGTAATGAGTTTTACCGTAATCGTTTTCGAGCTAAGCACAGCTCTGATCTTTTAATGAGATTTCAGGTTAGTTTAATCTATATAAACTTATATTTATGCTTCTCGTATTTTTTCGTAAATACATCTCCAAATGCATCTATGTATTAGAAAATGTTCTTTCTTATATTCATTAGTTTAGTTTGATTTTTTTTTTAATTTCGTGAAAGATAGCTTCAAAGAAGAAGATGAACAATTAATATTTTTGTTATTTTGATGGAAAATTAACGATGAAGAACAATAACAACAATAATTCTTCTTGCAATAAATTGATAGTGATGTGTGAATAAACGTATTCTTTTGTGCTTTCTCATTAGTCAATGACTCAAGCTAGCTCTTTAATTACAAATTAAATAATTTTATATATATCTCACGTAGTAATATAATATATATTTCATAATTATTACGTATAAATGAATCCAACATTTTTTTTAAACTTACGTTAAATTATCTAATGGACGTAAAAGCGTGTGTATTGAATTTTATTAACACTTTAAAATTGAATCTTTTGTAAGCTTGAAATATATAGAACATTAAATAAAACATCAATATCGTAAAATTTTATAGCCAATATTAATTAACAATGGACGAACAAACTATCTCTTAAAATATGTGAGGTTTATTGATTTTTTGGTTAATTGATTTTTGTAATTATGATACAAATATCAAACTTTTTTTCTTAACCCACCAACAATCATTTCAAAACCATGGTTAATATGATCATATTAGTGTACAATAATGTCATGTCACATCACATATATTAGATCTTTACGTTAATTAGATATGGTAGTTATAATTAAAAGGGTAGATTAAGTAATACAAGATGACAATAATTAAATAATTTTTTTTGAGAGTTTGGACCATGAGTTAATTATTGTTTTGTTGTTATAATAATTAAAAAATATTATTATCCTTTAATTCTATTAAAATTTGAAAACTTTTTCTAAGTTAAACATAATACTCAACCTATAAAATCATAGATTGAAGCATCTACTCTCATTTATTGTTGACTATTTAAAAGAAAAATTAGATGAAAACAAGAAAAAAATGTTAAAATATTAATTTCATCTCCATGCATTAAAGTTTGTTCATATTACAACTTCAAAAACAAATAGTGAAAAATCCAAATGGATAACATGATTTTTTTTAATTTTTTTTGGAAAATGATAGTAATAATAATAAATAAATAAACTTCCTATTGAAACCTTAAATGGGTTATATATAGTTTTAGAAGAAAGTAAGACTAATTAGTAATTAATTCTTAACTACTTATTAATCTAAGTTAACAATGAAACAAATATTAAACTTAAATAAGTTAGTTATATAATTCTCCTTCTAAATTTATGGTTTATTGTCAAATTGAATTTAGCGAGATGTGAAAATATTTACAAATACACGAAATTATCCCATGCTATCCATATTTTATAAATGTTTTGATTTATTTTACTATATAATTTGAGGATAATGGAAATATTAAGACTGTTAAATTTTGAAATATTGTATAGTTGAGAAAGGAGAATAATGGTGATGAGGTGAAACTACCAAGGCAAATAAAAATTACAAGTGAGGAGGAAATAAATGAAGAAGCAATTGAGAAGACATTGAGAAGAGGAATTAGATTTTACTCAACTTTACAAACACAAGATGGCTTTTGGCCTGGTGACTATGGTGGCCCTTTGTTTCTTCTTCCTGCCTTGGTAAATTTCTAG

mRNA sequence

ATGTGGAAGCTCAAAGTTTCGGAAGGATGGGAGACAAGTGAGAACGATCACGTAGGAAGACAATATTGGAAATTCGACACAAATCTCACACCTTCTGAAGAAGAAAAAGCTCAAATACAAAAGTTTTGTAATGAGTTTTACCGTAATCGTTTTCGAGCTAAGCACAGCTCTGATCTTTTAATGAGATTTCAGTTGAGAAAGGAGAATAATGGTGATGAGGTGAAACTACCAAGGCAAATAAAAATTACAAGTGAGGAGGAAATAAATGAAGAAGCAATTGAGAAGACATTGAGAAGAGGAATTAGATTTTACTCAACTTTACAAACACAAGATGGCTTTTGGCCTGGTGACTATGGTGGCCCTTTGTTTCTTCTTCCTGCCTTGGTAAATTTCTAG

Coding sequence (CDS)

ATGTGGAAGCTCAAAGTTTCGGAAGGATGGGAGACAAGTGAGAACGATCACGTAGGAAGACAATATTGGAAATTCGACACAAATCTCACACCTTCTGAAGAAGAAAAAGCTCAAATACAAAAGTTTTGTAATGAGTTTTACCGTAATCGTTTTCGAGCTAAGCACAGCTCTGATCTTTTAATGAGATTTCAGTTGAGAAAGGAGAATAATGGTGATGAGGTGAAACTACCAAGGCAAATAAAAATTACAAGTGAGGAGGAAATAAATGAAGAAGCAATTGAGAAGACATTGAGAAGAGGAATTAGATTTTACTCAACTTTACAAACACAAGATGGCTTTTGGCCTGGTGACTATGGTGGCCCTTTGTTTCTTCTTCCTGCCTTGGTAAATTTCTAG

Protein sequence

MWKLKVSEGWETSENDHVGRQYWKFDTNLTPSEEEKAQIQKFCNEFYRNRFRAKHSSDLLMRFQLRKENNGDEVKLPRQIKITSEEEINEEAIEKTLRRGIRFYSTLQTQDGFWPGDYGGPLFLLPALVNF*

Homology

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BLAST of Csa1G025800 vs. Swiss-Prot
Match: OXSC_CUCPE (Probable oxidosqualene cyclase OS=Cucurbita pepo GN=CPR PE=2 SV=1)

HSP 1 Score: 199.1 bits (505), Expect = 3.0e-50
Identity = 91/129 (70.54%), Postives = 107/129 (82.95%), Query Frame = 1


Query: 1   MWKLKVSEGWETSENDHVGRQYWKFDTNLTPSEEEKAQIQKFCNEFYRNRFRAKHSSDLL 60
           MW LK S+GWETS+N H+GRQ+W+FD NL PS EE+A++   CN+FY +RF+AKHSSDLL
Sbjct: 1   MWTLKFSKGWETSDNAHLGRQFWEFDPNLQPSLEEQARVHNVCNDFYTHRFQAKHSSDLL 60

Query: 61  MRFQLRKENNGDEVKLPRQIKITSEEEINEEAIEKTLRRGIRFYSTLQTQDGFWPGDYGG 120
           MR QL+K  NG EVKLP Q+K+ SEEE++EEA+E TLRR IRFYST+QTQDGFWPGDY G
Sbjct: 61  MRLQLKKA-NGSEVKLPTQVKLRSEEEMSEEAVETTLRRAIRFYSTMQTQDGFWPGDYAG 120

Query: 121 PLFLLPALV 130
           PLFLLP LV
Sbjct: 121 PLFLLPGLV 128

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BLAST of Csa1G025800 vs. Swiss-Prot
Match: LAS1_ARATH (Lanosterol synthase OS=Arabidopsis thaliana GN=LAS1 PE=2 SV=1)

HSP 1 Score: 147.5 bits (371), Expect = 1.0e-34
Identity = 77/131 (58.78%), Postives = 89/131 (67.94%), Query Frame = 1


Query: 1   MWKLKVSEGWETSENDHVGRQYWKFDTNLTPSEEEKAQIQKFCNEFYRNRFRAKHSSDLL 60
           MW+LK+SEG E S N HVGRQ+W++D     SEE +  I    + F  NRF +KHSSDLL
Sbjct: 1   MWRLKLSEGDEESVNQHVGRQFWEYDNQFGTSEE-RHHINHLRSNFTLNRFSSKHSSDLL 60

Query: 61  MRFQLRKENNGDEVKLPRQIKITSEEE--INEEAIEKTLRRGIRFYSTLQTQDGFWPGDY 120
            RFQ  KE      +LP Q+K+   EE  INEE +  TLRR +RFYS LQ+QDGFWPGDY
Sbjct: 61  YRFQCWKEKGKGMERLP-QVKVKEGEERLINEEVVNVTLRRSLRFYSILQSQDGFWPGDY 120

Query: 121 GGPLFLLPALV 130
           GGPLFLLPALV
Sbjct: 121 GGPLFLLPALV 129

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BLAST of Csa1G025800 vs. Swiss-Prot
Match: CAS1_PANGI (Cycloartenol Synthase OS=Panax ginseng GN=OSCPNX1 PE=1 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 7.3e-33
Identity = 71/132 (53.79%), Postives = 89/132 (67.42%), Query Frame = 1


Query: 1   MWKLKVSEG---WETSENDHVGRQYWKFDTNLTPSEEEKAQIQKFCNEFYRNRFRAKHSS 60
           MWKLK++EG   W  + NDHVGRQ W+FD N+  S EE A+++K    F  +RF  KHS+
Sbjct: 1   MWKLKIAEGGNPWLRTLNDHVGRQIWEFDPNIG-SPEELAEVEKVRENFRNHRFEKKHSA 60

Query: 61  DLLMRFQLRKENNGDEVKLPRQIKITSEEEINEEAIEKTLRRGIRFYSTLQTQDGFWPGD 120
           DLLMR Q   EN G  V LP Q+K+   E+I+E+ +  TL+R + FYSTLQ  DG WPGD
Sbjct: 61  DLLMRIQFANENPGSVV-LP-QVKVNDGEDISEDKVTVTLKRAMSFYSTLQAHDGHWPGD 120

Query: 121 YGGPLFLLPALV 130
           YGGP+FL+P LV
Sbjct: 121 YGGPMFLMPGLV 129

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BLAST of Csa1G025800 vs. Swiss-Prot
Match: CAS1_RICCO (Cycloartenol synthase OS=Ricinus communis PE=1 SV=1)

HSP 1 Score: 141.0 bits (354), Expect = 9.5e-33
Identity = 68/133 (51.13%), Postives = 92/133 (69.17%), Query Frame = 1


Query: 1   MWKLKVSEG----WETSENDHVGRQYWKFDTNLTPSEEEKAQIQKFCNEFYRNRFRAKHS 60
           MWKL+++EG    W  + NDH+GRQ W+FD++   S EE +QI+     F +NRF  KHS
Sbjct: 1   MWKLRIAEGSGNPWLRTTNDHIGRQVWEFDSSKIGSPEELSQIENARQNFTKNRFIHKHS 60

Query: 61  SDLLMRFQLRKENNGDEVKLPRQIKITSEEEINEEAIEKTLRRGIRFYSTLQTQDGFWPG 120
           SDLLMR Q  KEN   EV LP Q+K+   E++ EE ++ TLRR + +YS++Q  DG WPG
Sbjct: 61  SDLLMRIQFSKENPICEV-LP-QVKVKESEQVTEEKVKITLRRALNYYSSIQADDGHWPG 120

Query: 121 DYGGPLFLLPALV 130
           DYGGP+FL+P L+
Sbjct: 121 DYGGPMFLMPGLI 131

[Close]

BLAST of Csa1G025800 vs. Swiss-Prot
Match: CAS1_ARATH (Cycloartenol synthase OS=Arabidopsis thaliana GN=CAS1 PE=1 SV=2)

HSP 1 Score: 138.7 bits (348), Expect = 4.7e-32
Identity = 71/133 (53.38%), Postives = 91/133 (68.42%), Query Frame = 1


Query: 1   MWKLKVSEG---WETSENDHVGRQYWKFDTNL-TPSEEEKAQIQKFCNEFYRNRFRAKHS 60
           MWKLK++EG   W  + N+HVGRQ+W+FD NL TP  E+ A +++    F  NRF  KHS
Sbjct: 1   MWKLKIAEGGSPWLRTTNNHVGRQFWEFDPNLGTP--EDLAAVEEARKSFSDNRFVQKHS 60

Query: 61  SDLLMRFQLRKENNGDEVKLPRQIKITSEEEINEEAIEKTLRRGIRFYSTLQTQDGFWPG 120
           +DLLMR Q  +EN    V LP Q+KI   +++ EE +E TL+RG+ FYST+Q  DG WPG
Sbjct: 61  ADLLMRLQFSRENLISPV-LP-QVKIEDTDDVTEEMVETTLKRGLDFYSTIQAHDGHWPG 120

Query: 121 DYGGPLFLLPALV 130
           DYGGP+FLLP L+
Sbjct: 121 DYGGPMFLLPGLI 129

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BLAST of Csa1G025800 vs. TrEMBL
Match: A0A0A0LQ14_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G025800 PE=4 SV=1)

HSP 1 Score: 276.2 bits (705), Expect = 2.1e-71
Identity = 131/131 (100.00%), Postives = 131/131 (100.00%), Query Frame = 1


Query: 1   MWKLKVSEGWETSENDHVGRQYWKFDTNLTPSEEEKAQIQKFCNEFYRNRFRAKHSSDLL 60
           MWKLKVSEGWETSENDHVGRQYWKFDTNLTPSEEEKAQIQKFCNEFYRNRFRAKHSSDLL
Sbjct: 1   MWKLKVSEGWETSENDHVGRQYWKFDTNLTPSEEEKAQIQKFCNEFYRNRFRAKHSSDLL 60

Query: 61  MRFQLRKENNGDEVKLPRQIKITSEEEINEEAIEKTLRRGIRFYSTLQTQDGFWPGDYGG 120
           MRFQLRKENNGDEVKLPRQIKITSEEEINEEAIEKTLRRGIRFYSTLQTQDGFWPGDYGG
Sbjct: 61  MRFQLRKENNGDEVKLPRQIKITSEEEINEEAIEKTLRRGIRFYSTLQTQDGFWPGDYGG 120

Query: 121 PLFLLPALVNF 132
           PLFLLPALVNF
Sbjct: 121 PLFLLPALVNF 131

[Close]

BLAST of Csa1G025800 vs. TrEMBL
Match: Q9SSU5_LUFAE (Terpene cyclase/mutase family member OS=Luffa aegyptiaca GN=LcOSC2 PE=2 SV=1)

HSP 1 Score: 212.6 bits (540), Expect = 2.9e-52
Identity = 100/129 (77.52%), Postives = 111/129 (86.05%), Query Frame = 1


Query: 1   MWKLKVSEGWETSENDHVGRQYWKFDTNLTPSEEEKAQIQKFCNEFYRNRFRAKHSSDLL 60
           MWKLKVS+GWETSENDHVGRQYW+FD NL PSEEE+A+I+  CNEF++NRF  K SSDLL
Sbjct: 1   MWKLKVSKGWETSENDHVGRQYWEFDPNLEPSEEERAEIENVCNEFHKNRFHVKQSSDLL 60

Query: 61  MRFQLRKENNGDEVKLPRQIKITSEEEINEEAIEKTLRRGIRFYSTLQTQDGFWPGDYGG 120
           MR QLRKE N   VKL  QIK+ SEEEI+EEA+E TLRR IRFYST+QTQDGFWPGDYGG
Sbjct: 61  MRLQLRKE-NASNVKLLTQIKVASEEEISEEAVETTLRRAIRFYSTMQTQDGFWPGDYGG 120

Query: 121 PLFLLPALV 130
           PLFLLP LV
Sbjct: 121 PLFLLPGLV 128

[Close]

BLAST of Csa1G025800 vs. TrEMBL
Match: M5XWK0_PRUPE (Terpene cyclase/mutase family member OS=Prunus persica GN=PRUPE_ppa022710mg PE=3 SV=1)

HSP 1 Score: 165.2 bits (417), Expect = 5.3e-38
Identity = 84/134 (62.69%), Postives = 104/134 (77.61%), Query Frame = 1


Query: 1   MWKLKVSEG---WETSENDHVGRQYWKFDTNL-TPSEEEKAQIQKFCNEFYRNRFRAKHS 60
           MW+LK+SEG   W TS N+H+GRQYW+FD NL TP  EE+AQ++K  +EF +NRF++KHS
Sbjct: 1   MWRLKLSEGKDPWLTSVNNHIGRQYWEFDPNLGTP--EERAQVEKARHEFRKNRFQSKHS 60

Query: 61  SDLLMRFQLRKENNGDEVKLPRQIKITS-EEEINEEAIEKTLRRGIRFYSTLQTQDGFWP 120
           SDLLMR Q  KE   +  ++P Q+K  S EEEI+EEA+  TLRR + FYSTLQ +DGFWP
Sbjct: 61  SDLLMRLQFAKEKACEMKQVPAQVKARSDEEEISEEAVGITLRRALSFYSTLQAEDGFWP 120

Query: 121 GDYGGPLFLLPALV 130
           GDYGGPLFLLP LV
Sbjct: 121 GDYGGPLFLLPGLV 132

[Close]

BLAST of Csa1G025800 vs. TrEMBL
Match: U5GC23_POPTR (Terpene cyclase/mutase family member OS=Populus trichocarpa GN=POPTR_0006s07870g PE=3 SV=1)

HSP 1 Score: 162.9 bits (411), Expect = 2.6e-37
Identity = 83/133 (62.41%), Postives = 103/133 (77.44%), Query Frame = 1


Query: 1   MWKLKVSEG---WETSENDHVGRQYWKFDTNL-TPSEEEKAQIQKFCNEFYRNRFRAKHS 60
           MWKLK+SEG   W  S N+HVGRQ+W+FD +L TP  EE+AQ++ + NEF ++RF  KHS
Sbjct: 1   MWKLKLSEGNDPWLKSVNNHVGRQFWEFDPHLGTP--EERAQVENYRNEFTKSRFLTKHS 60

Query: 61  SDLLMRFQLRKENNGDEVKLPRQIKITSEEEINEEAIEKTLRRGIRFYSTLQTQDGFWPG 120
           SDLLMRFQ  +EN   E+KLP   K+ SEEEI +E ++ TLRR +RFYSTLQ +DGFWPG
Sbjct: 61  SDLLMRFQFARENPC-EMKLP-MAKVRSEEEITKEVVDTTLRRSLRFYSTLQAEDGFWPG 120

Query: 121 DYGGPLFLLPALV 130
           DYGGP+FLLP LV
Sbjct: 121 DYGGPMFLLPGLV 129

[Close]

BLAST of Csa1G025800 vs. TrEMBL
Match: A0A061GPF7_THECC (Terpene cyclase/mutase family member OS=Theobroma cacao GN=TCM_038749 PE=3 SV=1)

HSP 1 Score: 162.9 bits (411), Expect = 2.6e-37
Identity = 79/134 (58.96%), Postives = 102/134 (76.12%), Query Frame = 1


Query: 1   MWKLKVSEG---WETSENDHVGRQYWKFDTNL-TPSEEEKAQIQKFCNEFYRNRFRAKHS 60
           MWKLK+S+G   W  S N+H+GRQYW+FD NL TP  EE+A+++K  NEF +NRF+ K S
Sbjct: 1   MWKLKLSQGNEPWLKSVNNHIGRQYWEFDPNLGTP--EERARVEKARNEFTKNRFQTKQS 60

Query: 61  SDLLMRFQLRKENN-GDEVKLPRQIKITSEEEINEEAIEKTLRRGIRFYSTLQTQDGFWP 120
           SD+LMRFQ  +ENN   E KLP ++ + + +E++EE +  TLRR +R YSTLQ +DGFWP
Sbjct: 61  SDMLMRFQFARENNPAGERKLPDKVNVKTSQEVSEEMVRTTLRRALRLYSTLQCEDGFWP 120

Query: 121 GDYGGPLFLLPALV 130
           GDYGGPLFLLP LV
Sbjct: 121 GDYGGPLFLLPGLV 132

[Close]

BLAST of Csa1G025800 vs. TAIR10
Match: AT3G45130.1 (AT3G45130.1 lanosterol synthase 1)

HSP 1 Score: 147.5 bits (371), Expect = 5.7e-36
Identity = 77/131 (58.78%), Postives = 89/131 (67.94%), Query Frame = 1


Query: 1   MWKLKVSEGWETSENDHVGRQYWKFDTNLTPSEEEKAQIQKFCNEFYRNRFRAKHSSDLL 60
           MW+LK+SEG E S N HVGRQ+W++D     SEE +  I    + F  NRF +KHSSDLL
Sbjct: 1   MWRLKLSEGDEESVNQHVGRQFWEYDNQFGTSEE-RHHINHLRSNFTLNRFSSKHSSDLL 60

Query: 61  MRFQLRKENNGDEVKLPRQIKITSEEE--INEEAIEKTLRRGIRFYSTLQTQDGFWPGDY 120
            RFQ  KE      +LP Q+K+   EE  INEE +  TLRR +RFYS LQ+QDGFWPGDY
Sbjct: 61  YRFQCWKEKGKGMERLP-QVKVKEGEERLINEEVVNVTLRRSLRFYSILQSQDGFWPGDY 120

Query: 121 GGPLFLLPALV 130
           GGPLFLLPALV
Sbjct: 121 GGPLFLLPALV 129

[Close]

BLAST of Csa1G025800 vs. TAIR10
Match: AT2G07050.1 (AT2G07050.1 cycloartenol synthase 1)

HSP 1 Score: 138.7 bits (348), Expect = 2.7e-33
Identity = 71/133 (53.38%), Postives = 91/133 (68.42%), Query Frame = 1


Query: 1   MWKLKVSEG---WETSENDHVGRQYWKFDTNL-TPSEEEKAQIQKFCNEFYRNRFRAKHS 60
           MWKLK++EG   W  + N+HVGRQ+W+FD NL TP  E+ A +++    F  NRF  KHS
Sbjct: 1   MWKLKIAEGGSPWLRTTNNHVGRQFWEFDPNLGTP--EDLAAVEEARKSFSDNRFVQKHS 60

Query: 61  SDLLMRFQLRKENNGDEVKLPRQIKITSEEEINEEAIEKTLRRGIRFYSTLQTQDGFWPG 120
           +DLLMR Q  +EN    V LP Q+KI   +++ EE +E TL+RG+ FYST+Q  DG WPG
Sbjct: 61  ADLLMRLQFSRENLISPV-LP-QVKIEDTDDVTEEMVETTLKRGLDFYSTIQAHDGHWPG 120

Query: 121 DYGGPLFLLPALV 130
           DYGGP+FLLP L+
Sbjct: 121 DYGGPMFLLPGLI 129

[Close]

BLAST of Csa1G025800 vs. TAIR10
Match: AT1G78970.2 (AT1G78970.2 lupeol synthase 1)

HSP 1 Score: 106.7 bits (265), Expect = 1.1e-23
Identity = 60/134 (44.78%), Postives = 79/134 (58.96%), Query Frame = 1


Query: 1   MWKLKVSEGWET-----SENDHVGRQYWKFDTNLTPSEEEKAQIQKFCNEFYRNRFRAKH 60
           MWKLK+ +G        S N+ VGRQ WKFD +   S EE+A +++    F  NRFR K 
Sbjct: 1   MWKLKIGKGNGEDPHLFSSNNFVGRQTWKFD-HKAGSPEERAAVEEARRGFLDNRFRVKG 60

Query: 61  SSDLLMRFQLRKENNGDEVKLPRQIKITSEEEINEEAIEKTLRRGIRFYSTLQTQDGFWP 120
            SDLL R Q  +E   ++  +P Q+K T+ EEI  E     LRRG+R+++ LQ  DG WP
Sbjct: 61  CSDLLWRMQFLREKKFEQ-GIP-QLKATNIEEITYETTTNALRRGVRYFTALQASDGHWP 120

Query: 121 GDYGGPLFLLPALV 130
           G+  GPLF LP L+
Sbjct: 121 GEITGPLFFLPPLI 131

[Close]

BLAST of Csa1G025800 vs. TAIR10
Match: AT1G66960.1 (AT1G66960.1 Terpenoid cyclases family protein)

HSP 1 Score: 102.4 bits (254), Expect = 2.1e-22
Identity = 58/134 (43.28%), Postives = 75/134 (55.97%), Query Frame = 1


Query: 1   MWKLKVSEG-----WETSENDHVGRQYWKFDTNLTPSEEEKAQIQKFCNEFYRNRFRAKH 60
           MW+LKV EG     +  S N+ VGRQ W+FD     + EE+  +++    F+ NR R K 
Sbjct: 1   MWRLKVGEGKGKDPYLFSSNNFVGRQTWEFDPK-AGTREERTAVEEARRSFFDNRSRVKP 60

Query: 61  SSDLLMRFQLRKENNGDEVKLPRQIKITSEEEINEEAIEKTLRRGIRFYSTLQTQDGFWP 120
           SSDLL + Q  KE   ++V  P  +KI   E I  E     LRRG+ F S LQ  DG WP
Sbjct: 61  SSDLLWKMQFLKEAKFEQVIPP--VKIDGGEAITYEKATNALRRGVAFLSALQASDGHWP 120

Query: 121 GDYGGPLFLLPALV 130
           G++ GPL +LP LV
Sbjct: 121 GEFTGPLCMLPPLV 131

[Close]

BLAST of Csa1G025800 vs. TAIR10
Match: AT1G78950.1 (AT1G78950.1 Terpenoid cyclases family protein)

HSP 1 Score: 99.8 bits (247), Expect = 1.4e-21
Identity = 55/134 (41.04%), Postives = 72/134 (53.73%), Query Frame = 1


Query: 1   MWKLKVSEG-----WETSENDHVGRQYWKFDTNLTPSEEEKAQIQKFCNEFYRNRFRAKH 60
           MW+LK+ EG     +  + N+  GRQ W+FD +   S EE+  + +    FY NRF  K 
Sbjct: 1   MWRLKIGEGNGDDPYLFTTNNFAGRQTWEFDPD-GGSPEERHSVVEARRIFYDNRFHVKA 60

Query: 61  SSDLLMRFQLRKENNGDEVKLPRQIKITSEEEINEEAIEKTLRRGIRFYSTLQTQDGFWP 120
           SSDLL R Q  +E   ++   P  +K+   E++  E     LRRGI F+S LQ  DG WP
Sbjct: 61  SSDLLWRMQFLREKKFEQRIAP--VKVEDSEKVTFETATSALRRGIHFFSALQASDGHWP 120

Query: 121 GDYGGPLFLLPALV 130
            +  GPLF LP LV
Sbjct: 121 AENAGPLFFLPPLV 131

[Close]

BLAST of Csa1G025800 vs. NCBI nr
Match: gi|700208802|gb|KGN63898.1| (hypothetical protein Csa_1G025800 [Cucumis sativus])

HSP 1 Score: 276.2 bits (705), Expect = 3.0e-71
Identity = 131/131 (100.00%), Postives = 131/131 (100.00%), Query Frame = 1


Query: 1   MWKLKVSEGWETSENDHVGRQYWKFDTNLTPSEEEKAQIQKFCNEFYRNRFRAKHSSDLL 60
           MWKLKVSEGWETSENDHVGRQYWKFDTNLTPSEEEKAQIQKFCNEFYRNRFRAKHSSDLL
Sbjct: 1   MWKLKVSEGWETSENDHVGRQYWKFDTNLTPSEEEKAQIQKFCNEFYRNRFRAKHSSDLL 60

Query: 61  MRFQLRKENNGDEVKLPRQIKITSEEEINEEAIEKTLRRGIRFYSTLQTQDGFWPGDYGG 120
           MRFQLRKENNGDEVKLPRQIKITSEEEINEEAIEKTLRRGIRFYSTLQTQDGFWPGDYGG
Sbjct: 61  MRFQLRKENNGDEVKLPRQIKITSEEEINEEAIEKTLRRGIRFYSTLQTQDGFWPGDYGG 120

Query: 121 PLFLLPALVNF 132
           PLFLLPALVNF
Sbjct: 121 PLFLLPALVNF 131

[Close]

BLAST of Csa1G025800 vs. NCBI nr
Match: gi|778656570|ref|XP_011649297.1| (PREDICTED: probable oxidosqualene cyclase [Cucumis sativus])

HSP 1 Score: 271.6 bits (693), Expect = 7.5e-70
Identity = 129/129 (100.00%), Postives = 129/129 (100.00%), Query Frame = 1


Query: 1   MWKLKVSEGWETSENDHVGRQYWKFDTNLTPSEEEKAQIQKFCNEFYRNRFRAKHSSDLL 60
           MWKLKVSEGWETSENDHVGRQYWKFDTNLTPSEEEKAQIQKFCNEFYRNRFRAKHSSDLL
Sbjct: 1   MWKLKVSEGWETSENDHVGRQYWKFDTNLTPSEEEKAQIQKFCNEFYRNRFRAKHSSDLL 60

Query: 61  MRFQLRKENNGDEVKLPRQIKITSEEEINEEAIEKTLRRGIRFYSTLQTQDGFWPGDYGG 120
           MRFQLRKENNGDEVKLPRQIKITSEEEINEEAIEKTLRRGIRFYSTLQTQDGFWPGDYGG
Sbjct: 61  MRFQLRKENNGDEVKLPRQIKITSEEEINEEAIEKTLRRGIRFYSTLQTQDGFWPGDYGG 120

Query: 121 PLFLLPALV 130
           PLFLLPALV
Sbjct: 121 PLFLLPALV 129

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BLAST of Csa1G025800 vs. NCBI nr
Match: gi|659107098|ref|XP_008453521.1| (PREDICTED: probable oxidosqualene cyclase [Cucumis melo])

HSP 1 Score: 240.0 bits (611), Expect = 2.4e-60
Identity = 116/130 (89.23%), Postives = 123/130 (94.62%), Query Frame = 1


Query: 1   MWKLKVSEGWETSENDHVGRQYWKFDTNLTPSEEEKAQIQKFCNEFYRNRFRAKHSSDLL 60
           MWKL++S+G ETSEN+H+GRQYWKFDTNL PSEEEKAQIQKF NEFYRNRF+ KHSSDLL
Sbjct: 1   MWKLELSQGRETSENNHIGRQYWKFDTNLIPSEEEKAQIQKFYNEFYRNRFQVKHSSDLL 60

Query: 61  MRFQLRKENN-GDEVKLPRQIKITSEEEINEEAIEKTLRRGIRFYSTLQTQDGFWPGDYG 120
           MRFQ+RKENN  DEVKLP QIKITSEEEINEEAIEKTLRRGIRFYSTLQTQDGFWPGDYG
Sbjct: 61  MRFQMRKENNKDDEVKLPTQIKITSEEEINEEAIEKTLRRGIRFYSTLQTQDGFWPGDYG 120

Query: 121 GPLFLLPALV 130
           GPLFLLPALV
Sbjct: 121 GPLFLLPALV 130

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BLAST of Csa1G025800 vs. NCBI nr
Match: gi|6045135|dbj|BAA85267.1| (oxidosqualene cyclase [Luffa aegyptiaca])

HSP 1 Score: 212.6 bits (540), Expect = 4.1e-52
Identity = 100/129 (77.52%), Postives = 111/129 (86.05%), Query Frame = 1


Query: 1   MWKLKVSEGWETSENDHVGRQYWKFDTNLTPSEEEKAQIQKFCNEFYRNRFRAKHSSDLL 60
           MWKLKVS+GWETSENDHVGRQYW+FD NL PSEEE+A+I+  CNEF++NRF  K SSDLL
Sbjct: 1   MWKLKVSKGWETSENDHVGRQYWEFDPNLEPSEEERAEIENVCNEFHKNRFHVKQSSDLL 60

Query: 61  MRFQLRKENNGDEVKLPRQIKITSEEEINEEAIEKTLRRGIRFYSTLQTQDGFWPGDYGG 120
           MR QLRKE N   VKL  QIK+ SEEEI+EEA+E TLRR IRFYST+QTQDGFWPGDYGG
Sbjct: 61  MRLQLRKE-NASNVKLLTQIKVASEEEISEEAVETTLRRAIRFYSTMQTQDGFWPGDYGG 120

Query: 121 PLFLLPALV 130
           PLFLLP LV
Sbjct: 121 PLFLLPGLV 128

[Close]

BLAST of Csa1G025800 vs. NCBI nr
Match: gi|75254647|sp|Q6BE23.1|OXSC_CUCPE (RecName: Full=Probable oxidosqualene cyclase)

HSP 1 Score: 199.1 bits (505), Expect = 4.7e-48
Identity = 91/129 (70.54%), Postives = 109/129 (84.50%), Query Frame = 1


Query: 1   MWKLKVSEGWETSENDHVGRQYWKFDTNLTPSEEEKAQIQKFCNEFYRNRFRAKHSSDLL 60
           MW LK S+GWETS+N H+GRQ+W+FD NL PS EE+A++   CN+FY +RF+AKHSSDLL
Sbjct: 1   MWTLKFSKGWETSDNAHLGRQFWEFDPNLQPSLEEQARVHNVCNDFYTHRFQAKHSSDLL 60

Query: 61  MRFQLRKENNGDEVKLPRQIKITSEEEINEEAIEKTLRRGIRFYSTLQTQDGFWPGDYGG 120
           MR QL+K  NG EVKLP Q+K+ SEEE++EEA+E TLRR IRFYST+QTQDGFWPGDY G
Sbjct: 61  MRLQLKKA-NGSEVKLPTQVKLRSEEEMSEEAVETTLRRAIRFYSTMQTQDGFWPGDYAG 120

Query: 121 PLFLLPALV 130
           PLFLLP LV
Sbjct: 121 PLFLLPGLV 128

The following BLAST results are available for this feature:

BLAST of Csa1G025800 vs. Swiss-Prot


Match Name	E-value	Identity	Description
OXSC_CUCPE	3.0e-50	70.54	Probable oxidosqualene cyclase OS=Cucurbita pepo GN=CPR PE=2 SV=1	[more]
LAS1_ARATH	1.0e-34	58.78	Lanosterol synthase OS=Arabidopsis thaliana GN=LAS1 PE=2 SV=1	[more]
CAS1_PANGI	7.3e-33	53.79	Cycloartenol Synthase OS=Panax ginseng GN=OSCPNX1 PE=1 SV=1	[more]
CAS1_RICCO	9.5e-33	51.13	Cycloartenol synthase OS=Ricinus communis PE=1 SV=1	[more]
CAS1_ARATH	4.7e-32	53.38	Cycloartenol synthase OS=Arabidopsis thaliana GN=CAS1 PE=1 SV=2	[more]

BLAST of Csa1G025800 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LQ14_CUCSA	2.1e-71	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G025800 PE=4 SV=1	[more]
Q9SSU5_LUFAE	2.9e-52	77.52	Terpene cyclase/mutase family member OS=Luffa aegyptiaca GN=LcOSC2 PE=2 SV=1	[more]
M5XWK0_PRUPE	5.3e-38	62.69	Terpene cyclase/mutase family member OS=Prunus persica GN=PRUPE_ppa022710mg PE=3...	[more]
U5GC23_POPTR	2.6e-37	62.41	Terpene cyclase/mutase family member OS=Populus trichocarpa GN=POPTR_0006s07870g...	[more]
A0A061GPF7_THECC	2.6e-37	58.96	Terpene cyclase/mutase family member OS=Theobroma cacao GN=TCM_038749 PE=3 SV=1	[more]

BLAST of Csa1G025800 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G45130.1	5.7e-36	58.78	lanosterol synthase 1	[more]
AT2G07050.1	2.7e-33	53.38	cycloartenol synthase 1	[more]
AT1G78970.2	1.1e-23	44.78	lupeol synthase 1	[more]
AT1G66960.1	2.1e-22	43.28	Terpenoid cyclases family protein	[more]
AT1G78950.1	1.4e-21	41.04	Terpenoid cyclases family protein	[more]

BLAST of Csa1G025800 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700208802\|gb\|KGN63898.1\|	3.0e-71	100.00	hypothetical protein Csa_1G025800 [Cucumis sativus]	[more]
gi\|778656570\|ref\|XP_011649297.1\|	7.5e-70	100.00	PREDICTED: probable oxidosqualene cyclase [Cucumis sativus]	[more]
gi\|659107098\|ref\|XP_008453521.1\|	2.4e-60	89.23	PREDICTED: probable oxidosqualene cyclase [Cucumis melo]	[more]
gi\|6045135\|dbj\|BAA85267.1\|	4.1e-52	77.52	oxidosqualene cyclase [Luffa aegyptiaca]	[more]
gi\|75254647\|sp\|Q6BE23.1\|OXSC_CUCPE	4.7e-48	70.54	RecName: Full=Probable oxidosqualene cyclase	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR008930	Terpenoid_cyclase/PrenylTrfase

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008152	metabolic process
biological_process	GO:0006694	steroid biosynthetic process
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0016866	intramolecular transferase activity
molecular_function	GO:0000250	lanosterol synthase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Csa1G025800.1	Csa1G025800.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008930	Terpenoid cyclases/protein prenyltransferase alpha-alpha toroid	GENE3D	G3DSA:1.50.10.20		coord: 15..129 score: 5.5
IPR008930	Terpenoid cyclases/protein prenyltransferase alpha-alpha toroid	unknown	SSF48239	Terpenoid cyclases/Protein prenyltransferases	coord: 21..126 score: 8.79
None	No IPR available	PANTHER	PTHR11764	LANOSTEROL SYNTHASE	coord: 2..129 score: 4.6

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Csa1G025800	Cla018902	Watermelon (97103) v1	cuwmB095
Csa1G025800	ClCG06G014110	Watermelon (Charleston Gray)	cuwcgB082
Csa1G025800	Lsi06G012580	Bottle gourd (USVL1VR-Ls)	culsiB071
Csa1G025800	MELO3C029738.2	Melon (DHL92) v3.6.1	cumedB047
Csa1G025800	Cla97C06G123570	Watermelon (97103) v2	cuwmbB079
Csa1G025800	Bhi02G000125	Wax gourd	cuwgoB077
Csa1G025800	CSPI01G04570	Wild cucumber (PI 183967)	cpicuB000
Csa1G025800	Cucsa.111780	Cucumber (Gy14) v1	cgycuB137
Csa1G025800	CmaCh10G003400	Cucurbita maxima (Rimu)	cmacuB050
Csa1G025800	CmoCh10G003700	Cucurbita moschata (Rifu)	cmocuB039
Csa1G025800	Cp4.1LG18g06700	Cucurbita pepo (Zucchini)	cpecuB363
Csa1G025800	CsGy1G004470	Cucumber (Gy14) v2	cgybcuB001
Csa1G025800	Carg15189	Silver-seed gourd	carcuB0801

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Csa1G025800	Melon (DHL92) v3.5.1	cumeB055
Csa1G025800	Cucurbita pepo (Zucchini)	cpecuB654
Csa1G025800	Silver-seed gourd	carcuB0622
Csa1G025800	Cucurbita maxima (Rimu)	cmacuB087
Csa1G025800	Cucurbita moschata (Rifu)	cmocuB076