CsGy1G032140 (gene) Cucumber (Gy14) v2

Overview
NameCsGy1G032140
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v2)
Descriptionralf-like 25
LocationChr1 : 31384227 .. 31384433 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGTCATGGTTGATATGTGTGGTGTTTATTAGCATGTTGGTGATGAGCCAAGAAGTTGGAGCACAGACAAACCATCTTAAATTTCCTAATCCATGCGATCTTCCTTCTCCACCTCCAAGTTGTTCGTCAAAAGTTAAAGATCATGCTCCTACTAATCCCTATGATAGAGGTTGCTCTGCAATTCATCGATGTCGTGGAGATTGA

mRNA sequence

ATGAAGTCATGGTTGATATGTGTGGTGTTTATTAGCATGTTGGTGATGAGCCAAGAAGTTGGAGCACAGACAAACCATCTTAAATTTCCTAATCCATGCGATCTTCCTTCTCCACCTCCAAGTTGTTCGTCAAAAGTTAAAGATCATGCTCCTACTAATCCCTATGATAGAGGTTGCTCTGCAATTCATCGATGTCGTGGAGATTGA

Coding sequence (CDS)

ATGAAGTCATGGTTGATATGTGTGGTGTTTATTAGCATGTTGGTGATGAGCCAAGAAGTTGGAGCACAGACAAACCATCTTAAATTTCCTAATCCATGCGATCTTCCTTCTCCACCTCCAAGTTGTTCGTCAAAAGTTAAAGATCATGCTCCTACTAATCCCTATGATAGAGGTTGCTCTGCAATTCATCGATGTCGTGGAGATTGA

Protein sequence

MKSWLICVVFISMLVMSQEVGAQTNHLKFPNPCDLPSPPPSCSSKVKDHAPTNPYDRGCSAIHRCRGD
Homology
[Close]
BLAST of CsGy1G032140 vs. NCBI nr
Match: KGN66777.1 (hypothetical protein Csa_1G690160 [Cucumis sativus])

HSP 1 Score: 148.3 bits (373), Expect = 9.6e-33
Identity = 68/68 (100.00%), Postives = 68/68 (100.00%), Query Frame = 0 

Query: 1  MKSWLICVVFISMLVMSQEVGAQTNHLKFPNPCDLPSPPPSCSSKVKDHAPTNPYDRGCS 60
          MKSWLICVVFISMLVMSQEVGAQTNHLKFPNPCDLPSPPPSCSSKVKDHAPTNPYDRGCS
Sbjct: 1  MKSWLICVVFISMLVMSQEVGAQTNHLKFPNPCDLPSPPPSCSSKVKDHAPTNPYDRGCS 60

Query: 61 AIHRCRGD 69
          AIHRCRGD
Sbjct: 61 AIHRCRGD 68

[Close]
BLAST of CsGy1G032140 vs. NCBI nr
Match: XP_022947821.1 (protein RALF-like 10 [Cucurbita moschata])

HSP 1 Score: 88.2 bits (217), Expect = 1.2e-14
Identity = 40/67 (59.70%), Postives = 50/67 (74.63%), Query Frame = 0 

Query: 1  MKSWLICVVFISMLVMSQEVGAQTNHLKFPNPCDLPSPPPSCSSKVKDHAPTNPYDRGCS 60
          M+SWLIC+V I MLV+SQ+V ++T  LKFP+PC  P+PPP CS  + +  P NPY RGCS
Sbjct: 1  MRSWLICLVLIGMLVVSQDVASETKFLKFPDPCQQPNPPPGCS--IGNAVPANPYRRGCS 60

Query: 61 AIHRCRG 68
           I RCRG
Sbjct: 61 KITRCRG 65

[Close]
BLAST of CsGy1G032140 vs. NCBI nr
Match: KGN61625.1 (hypothetical protein Csa_2G191310 [Cucumis sativus])

HSP 1 Score: 87.8 bits (216), Expect = 1.5e-14
Identity = 41/67 (61.19%), Postives = 51/67 (76.12%), Query Frame = 0 

Query: 1  MKSWLICVVFISMLVMSQEVGAQTNHLKFPNPCDLPSPPPSCSSKVKDHAPTNPYDRGCS 60
          MKSWLIC+  I MLV+ QEV A+T HLKF +PC+ PSPPP+C ++ K     NPY+R CS
Sbjct: 1  MKSWLICLALIGMLVVGQEVAAETKHLKFIDPCNQPSPPPTCPNRDKVE-EANPYNRDCS 60

Query: 61 AIHRCRG 68
          AI+RCRG
Sbjct: 61 AINRCRG 66

[Close]
BLAST of CsGy1G032140 vs. NCBI nr
Match: XP_023533089.1 (protein RALF-like 10 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 87.8 bits (216), Expect = 1.5e-14
Identity = 41/67 (61.19%), Postives = 50/67 (74.63%), Query Frame = 0 

Query: 1  MKSWLICVVFISMLVMSQEVGAQTNHLKFPNPCDLPSPPPSCSSKVKDHAPTNPYDRGCS 60
          M+SWLIC+V ISMLV+SQEV ++T  LKFP+PC  P+PP  CS  + +  P NPY RGCS
Sbjct: 1  MRSWLICIVLISMLVVSQEVASETKFLKFPDPCQQPNPPLGCS--IGNAVPANPYRRGCS 60

Query: 61 AIHRCRG 68
           I RCRG
Sbjct: 61 KITRCRG 65

[Close]
BLAST of CsGy1G032140 vs. NCBI nr
Match: XP_016900883.1 (PREDICTED: protein RALF-like 30 [Cucumis melo])

HSP 1 Score: 75.1 bits (183), Expect = 1.0e-10
Identity = 39/67 (58.21%), Postives = 44/67 (65.67%), Query Frame = 0 

Query: 1  MKSWLICVVFISMLVMSQEVGAQTNHLKFPNPCDLPSPPPSCSSKVKDHAPTNPYDRGCS 60
          MKSWLIC   +SMLVM  EV A+T  LKF +PC  PSPP SC    +  +P   YDRGCS
Sbjct: 1  MKSWLICFALLSMLVMGHEVAAKTKFLKFLDPCLQPSPPRSCYKHERVDSPI-AYDRGCS 60

Query: 61 AIHRCRG 68
           I RCRG
Sbjct: 61 PILRCRG 66

[Close]
BLAST of CsGy1G032140 vs. TAIR10
Match: AT2G19030.1 (ralf-like 11)

HSP 1 Score: 60.1 bits (144), Expect = 6.2e-10
Identity = 31/73 (42.47%), Postives = 48/73 (65.75%), Query Frame = 0 

Query: 1  MKSWLICVVFISMLVMSQEVGAQTNHLKFP--NPCDLPSPPPSCS---SKVKDHAPTNPY 60
          MK+WLIC++ I   V+++ V ++ N++++   N C  P+PPP C+   ++ K+  P N Y
Sbjct: 1  MKAWLICLLVICAAVIAEPVESR-NYIEYGAINKCAGPNPPPGCNPPGAEQKNPTPVNEY 60

Query: 61 DRGCSAIHRCRGD 69
           RGCS IHRCR D
Sbjct: 61 SRGCSKIHRCRRD 72

[Close]
BLAST of CsGy1G032140 vs. TAIR10
Match: AT2G19045.1 (RALF-like 13)

HSP 1 Score: 58.2 bits (139), Expect = 2.4e-09
Identity = 30/73 (41.10%), Postives = 48/73 (65.75%), Query Frame = 0 

Query: 1  MKSWLICVVFISMLVMSQEVGAQTNHLKFP--NPCDLPSPPPSCS---SKVKDHAPTNPY 60
          MK+W+IC++ I   V+++ V ++ N++++   N C  P+PPP C+   ++ K+  P N Y
Sbjct: 1  MKAWVICLLVICAAVIAEPVESR-NYIEYGAINKCAGPNPPPGCNPPGAEQKNPNPVNEY 60

Query: 61 DRGCSAIHRCRGD 69
           RGCS IHRCR D
Sbjct: 61 SRGCSKIHRCRRD 72

[Close]
BLAST of CsGy1G032140 vs. TAIR10
Match: AT2G19040.1 (RALF-like 12)

HSP 1 Score: 55.1 bits (131), Expect = 2.0e-08
Identity = 29/73 (39.73%), Postives = 48/73 (65.75%), Query Frame = 0 

Query: 1  MKSWLICVVFISMLVMSQEVGAQTNHLKFP--NPCDLPSPPPSCS---SKVKDHAPTNPY 60
          MK+W+I ++ I  +V+++ V ++ N++++   N C  P+PPP C+   ++ K+  P N Y
Sbjct: 1  MKAWVIGLLVICAVVIAEPVESR-NYIEYGAINKCAGPNPPPGCNPPGTEQKNPTPVNEY 60

Query: 61 DRGCSAIHRCRGD 69
           RGCS IHRCR D
Sbjct: 61 SRGCSKIHRCRRD 72

[Close]
BLAST of CsGy1G032140 vs. TAIR10
Match: AT4G13075.1 (RALF-like 30)

HSP 1 Score: 42.7 bits (99), Expect = 1.0e-04
Identity = 28/73 (38.36%), Postives = 37/73 (50.68%), Query Frame = 0 

Query: 1  MKSWLICVVFISMLVMSQEVGAQTNHLKFPN-----PCDLPSPPPSC---SSKVKDHAPT 60
          MK+W+IC++ IS+ +M +   A     KF N     PC  P+PPP C    S  K     
Sbjct: 1  MKAWVICLMVISIFMMIEPTLAAGGG-KFLNPGVLDPCLRPNPPPECQAPGSAGKPRERV 60

Query: 61 NPYDRGCSAIHRC 66
          N Y  GCS + RC
Sbjct: 61 NEYKVGCSKLTRC 72

[Close]
BLAST of CsGy1G032140 vs. TAIR10
Match: AT3G04735.1 (RALF-like 21)

HSP 1 Score: 41.6 bits (96), Expect = 2.3e-04
Identity = 27/63 (42.86%), Postives = 31/63 (49.21%), Query Frame = 0 

Query: 6  ICVVFISMLVMSQEVGAQTNHLKFPNPCDLPSPPPSCSSKVKDHAPTNPYDRGCSAIHRC 65
          I  VFIS + M+         LK   PCD    P +C+       P NPY RGCS IHRC
Sbjct: 17 IACVFISSMNMTVGKVIGYPGLKPDLPCDHHRYPSACA---PSEQPVNPYRRGCSKIHRC 76

Query: 66 RGD 69
          R D
Sbjct: 77 RRD 76

[Close]
BLAST of CsGy1G032140 vs. Swiss-Prot
Match: sp|O64466|RLF11_ARATH (Protein RALF-like 11 OS=Arabidopsis thaliana OX=3702 GN=RALFL11 PE=3 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 1.1e-08
Identity = 31/73 (42.47%), Postives = 48/73 (65.75%), Query Frame = 0 

Query: 1  MKSWLICVVFISMLVMSQEVGAQTNHLKFP--NPCDLPSPPPSCS---SKVKDHAPTNPY 60
          MK+WLIC++ I   V+++ V ++ N++++   N C  P+PPP C+   ++ K+  P N Y
Sbjct: 1  MKAWLICLLVICAAVIAEPVESR-NYIEYGAINKCAGPNPPPGCNPPGAEQKNPTPVNEY 60

Query: 61 DRGCSAIHRCRGD 69
           RGCS IHRCR D
Sbjct: 61 SRGCSKIHRCRRD 72

[Close]
BLAST of CsGy1G032140 vs. Swiss-Prot
Match: sp|F4ISE2|RLF13_ARATH (Protein RALF-like 13 OS=Arabidopsis thaliana OX=3702 GN=RALFL13 PE=3 SV=1)

HSP 1 Score: 58.2 bits (139), Expect = 4.3e-08
Identity = 30/73 (41.10%), Postives = 48/73 (65.75%), Query Frame = 0 

Query: 1  MKSWLICVVFISMLVMSQEVGAQTNHLKFP--NPCDLPSPPPSCS---SKVKDHAPTNPY 60
          MK+W+IC++ I   V+++ V ++ N++++   N C  P+PPP C+   ++ K+  P N Y
Sbjct: 1  MKAWVICLLVICAAVIAEPVESR-NYIEYGAINKCAGPNPPPGCNPPGAEQKNPNPVNEY 60

Query: 61 DRGCSAIHRCRGD 69
           RGCS IHRCR D
Sbjct: 61 SRGCSKIHRCRRD 72

[Close]
BLAST of CsGy1G032140 vs. Swiss-Prot
Match: sp|F4ISE1|RLF12_ARATH (Protein RALF-like 12 OS=Arabidopsis thaliana OX=3702 GN=RALFL12 PE=2 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 3.6e-07
Identity = 29/73 (39.73%), Postives = 48/73 (65.75%), Query Frame = 0 

Query: 1  MKSWLICVVFISMLVMSQEVGAQTNHLKFP--NPCDLPSPPPSCS---SKVKDHAPTNPY 60
          MK+W+I ++ I  +V+++ V ++ N++++   N C  P+PPP C+   ++ K+  P N Y
Sbjct: 1  MKAWVIGLLVICAVVIAEPVESR-NYIEYGAINKCAGPNPPPGCNPPGTEQKNPTPVNEY 60

Query: 61 DRGCSAIHRCRGD 69
           RGCS IHRCR D
Sbjct: 61 SRGCSKIHRCRRD 72

[Close]
BLAST of CsGy1G032140 vs. TrEMBL
Match: tr|A0A0A0M0X3|A0A0A0M0X3_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G690160 PE=4 SV=1)

HSP 1 Score: 148.3 bits (373), Expect = 6.3e-33
Identity = 68/68 (100.00%), Postives = 68/68 (100.00%), Query Frame = 0 

Query: 1  MKSWLICVVFISMLVMSQEVGAQTNHLKFPNPCDLPSPPPSCSSKVKDHAPTNPYDRGCS 60
          MKSWLICVVFISMLVMSQEVGAQTNHLKFPNPCDLPSPPPSCSSKVKDHAPTNPYDRGCS
Sbjct: 1  MKSWLICVVFISMLVMSQEVGAQTNHLKFPNPCDLPSPPPSCSSKVKDHAPTNPYDRGCS 60

Query: 61 AIHRCRGD 69
          AIHRCRGD
Sbjct: 61 AIHRCRGD 68

[Close]
BLAST of CsGy1G032140 vs. TrEMBL
Match: tr|A0A0A0LI94|A0A0A0LI94_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G191310 PE=4 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 1.0e-14
Identity = 41/67 (61.19%), Postives = 51/67 (76.12%), Query Frame = 0 

Query: 1  MKSWLICVVFISMLVMSQEVGAQTNHLKFPNPCDLPSPPPSCSSKVKDHAPTNPYDRGCS 60
          MKSWLIC+  I MLV+ QEV A+T HLKF +PC+ PSPPP+C ++ K     NPY+R CS
Sbjct: 1  MKSWLICLALIGMLVVGQEVAAETKHLKFIDPCNQPSPPPTCPNRDKVE-EANPYNRDCS 60

Query: 61 AIHRCRG 68
          AI+RCRG
Sbjct: 61 AINRCRG 66

[Close]
BLAST of CsGy1G032140 vs. TrEMBL
Match: tr|A0A1S4DY33|A0A1S4DY33_CUCME (protein RALF-like 30 OS=Cucumis melo OX=3656 GN=LOC107991083 PE=4 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 6.8e-11
Identity = 39/67 (58.21%), Postives = 44/67 (65.67%), Query Frame = 0 

Query: 1  MKSWLICVVFISMLVMSQEVGAQTNHLKFPNPCDLPSPPPSCSSKVKDHAPTNPYDRGCS 60
          MKSWLIC   +SMLVM  EV A+T  LKF +PC  PSPP SC    +  +P   YDRGCS
Sbjct: 1  MKSWLICFALLSMLVMGHEVAAKTKFLKFLDPCLQPSPPRSCYKHERVDSPI-AYDRGCS 60

Query: 61 AIHRCRG 68
           I RCRG
Sbjct: 61 PILRCRG 66

[Close]
BLAST of CsGy1G032140 vs. TrEMBL
Match: tr|A0A0A0LZK3|A0A0A0LZK3_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G610770 PE=4 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 2.6e-10
Identity = 38/67 (56.72%), Postives = 43/67 (64.18%), Query Frame = 0 

Query: 1  MKSWLICVVFISMLVMSQEVGAQTNHLKFPNPCDLPSPPPSCSSKVKDHAPTNPYDRGCS 60
          MKSWLIC   +SMLVMS EV A+   L F + C  PSPP SC    +  +P  PYDRGCS
Sbjct: 34 MKSWLICFALLSMLVMSHEVAAEGKFLNFSDLCLQPSPPKSCYKDERADSPI-PYDRGCS 93

Query: 61 AIHRCRG 68
           I RCRG
Sbjct: 94 PILRCRG 99

[Close]
BLAST of CsGy1G032140 vs. TrEMBL
Match: tr|A0A178VY78|A0A178VY78_ARATH (RALFL12 OS=Arabidopsis thaliana OX=3702 GN=AXX17_At2g14390 PE=4 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 2.3e-06
Identity = 30/73 (41.10%), Postives = 49/73 (67.12%), Query Frame = 0 

Query: 1  MKSWLICVVFISMLVMSQEVGAQTNHLKFP--NPCDLPSPPPSCS---SKVKDHAPTNPY 60
          MK+W+IC++ I  +V+++ V ++ N++++   N C  P+PPP C+   ++ K+  P N Y
Sbjct: 1  MKAWVICLLLICAVVIAEPVESR-NYIEYGAINKCAGPNPPPGCNPPGTEQKNPTPVNEY 60

Query: 61 DRGCSAIHRCRGD 69
           RGCS IHRCR D
Sbjct: 61 SRGCSKIHRCRRD 72

The following BLAST results are available for this feature:
BLAST of CsGy1G032140 vs. NCBI nr
Match NameE-valueIdentityDescription
KGN66777.19.6e-33100.00hypothetical protein Csa_1G690160 [Cucumis sativus][more]
XP_022947821.11.2e-1459.70protein RALF-like 10 [Cucurbita moschata][more]
KGN61625.11.5e-1461.19hypothetical protein Csa_2G191310 [Cucumis sativus][more]
XP_023533089.11.5e-1461.19protein RALF-like 10 [Cucurbita pepo subsp. pepo][more]
XP_016900883.11.0e-1058.21PREDICTED: protein RALF-like 30 [Cucumis melo][more]
BLAST of CsGy1G032140 vs. TAIR10
Match NameE-valueIdentityDescription
AT2G19030.16.2e-1042.47ralf-like 11[more]
AT2G19045.12.4e-0941.10RALF-like 13[more]
AT2G19040.12.0e-0839.73RALF-like 12[more]
AT4G13075.11.0e-0438.36RALF-like 30[more]
AT3G04735.12.3e-0442.86RALF-like 21[more]
BLAST of CsGy1G032140 vs. Swiss-Prot
Match NameE-valueIdentityDescription
sp|O64466|RLF11_ARATH1.1e-0842.47Protein RALF-like 11 OS=Arabidopsis thaliana OX=3702 GN=RALFL11 PE=3 SV=1[more]
sp|F4ISE2|RLF13_ARATH4.3e-0841.10Protein RALF-like 13 OS=Arabidopsis thaliana OX=3702 GN=RALFL13 PE=3 SV=1[more]
sp|F4ISE1|RLF12_ARATH3.6e-0739.73Protein RALF-like 12 OS=Arabidopsis thaliana OX=3702 GN=RALFL12 PE=2 SV=1[more]
BLAST of CsGy1G032140 vs. TrEMBL
Match NameE-valueIdentityDescription
tr|A0A0A0M0X3|A0A0A0M0X3_CUCSA6.3e-33100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G690160 PE=4 SV=1[more]
tr|A0A0A0LI94|A0A0A0LI94_CUCSA1.0e-1461.19Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G191310 PE=4 SV=1[more]
tr|A0A1S4DY33|A0A1S4DY33_CUCME6.8e-1158.21protein RALF-like 30 OS=Cucumis melo OX=3656 GN=LOC107991083 PE=4 SV=1[more]
tr|A0A0A0LZK3|A0A0A0LZK3_CUCSA2.6e-1056.72Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G610770 PE=4 SV=1[more]
tr|A0A178VY78|A0A178VY78_ARATH2.3e-0641.10RALFL12 OS=Arabidopsis thaliana OX=3702 GN=AXX17_At2g14390 PE=4 SV=1[more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR008801RALF
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019722 calcium-mediated signaling
biological_process GO:0007267 cell-cell signaling
cellular_component GO:0048046 apoplast
cellular_component GO:0000793 condensed chromosome
molecular_function GO:0005179 hormone activity
molecular_function GO:0004871 signal transducer activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy1G032140.1CsGy1G032140.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber Gy14 genome (v2)
Date Performed: 2018-09-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008801Rapid ALkalinization FactorPFAMPF05498RALFcoord: 32..67
e-value: 2.9E-9
score: 37.5

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
CsGy1G032140Csa1G690160Cucumber (Chinese Long) v2cgybcuB002
CsGy1G032140CsaV3_1G045440Cucumber (Chinese Long) v3cgybcucB001
CsGy1G032140CsaV3_UNG233300Cucumber (Chinese Long) v3cgybcucB024
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
CsGy1G032140Watermelon (97103) v2cgybwmbB079
CsGy1G032140Wax gourdcgybwgoB100
CsGy1G032140Cucurbita maxima (Rimu)cgybcmaB019
CsGy1G032140Cucurbita maxima (Rimu)cgybcmaB027
CsGy1G032140Cucurbita moschata (Rifu)cgybcmoB018
CsGy1G032140Cucurbita moschata (Rifu)cgybcmoB027
CsGy1G032140Cucurbita pepo (Zucchini)cgybcpeB112
CsGy1G032140Cucurbita pepo (Zucchini)cgybcpeB135
CsGy1G032140Bottle gourd (USVL1VR-Ls)cgyblsiB055
CsGy1G032140Melon (DHL92) v3.5.1cgybmeB041
CsGy1G032140Melon (DHL92) v3.6.1cgybmedB035
CsGy1G032140Watermelon (Charleston Gray)cgybwcgB081
CsGy1G032140Wild cucumber (PI 183967)cgybcpiB002
CsGy1G032140Silver-seed gourdcarcgybB0899