Cp4.1LG20g05490 (gene) Cucurbita pepo (Zucchini)

NameCp4.1LG20g05490
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
Descriptionalpha/beta-Hydrolases superfamily protein
LocationCp4.1LG20 : 3203069 .. 3204661 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGGGGAGAATCGTGACGCCGAGAGGAGTCCCACTGAAAAGAGTAACGATGTTAAGAAACGGAACGAAGAAGACGAAATGGTATTGGAAATTGAAATGGGGGATCCGATTAAGAGCCATAAAAAATGCTCTCTCTTCTTCCTTACACAACCAGCGCCGCCGTTTGACATGTGCTACCGCCACCGGCCAACTAACTCCCGTCAACGGTGCAGCGTCGCCGCTTGTAATTCAGCCATCGAAATCAACCTCTAAGAAAAACGACCTCCGTTTGGCAAAGCCACTCGCCTCGCTCCTCCGTATGCCACAACGCGCCGCTGACTTCGTCGACTACGGCAACCACATGACCCCAACATTCTCCCCTCGTGATAAAATCGCCGATGTATGGCGTGACCTCCACGGCGGTTCTAATTGGGAAGGTCTACTAGATCCCCTCCACCCCTTCCTCCGCCGCGAGCTTGTTAAATACGGCGAGTTCGCTCAAGCCACCTACGACGCTTTTGACTTCGACCCACTTTCTGAATTCCGCGGCAGCTGTAGATACAACCGCCATAAGCTCCTCCACGAATTGGGTCTGGCTCAAAACGGCTACAAAGTGACTAGATACATCTACGCCTTGTCCCCTGTTGACGGTCCGGATTGGTTCGAGAGTTCCAAAATCGGCGAGGTGTGGAGCAGAGATTCGAATTGGATGGGGTTTGTAGCCGTTAGTAACGACGAGGAGAGCGAGAGAATCGGGCGGCGGGATATCCTCGTGGCCTGGAGAGGAACGGTAACCCCAACGGAATGGTACATCGATCTGAAAACCAAGCTGAAAAAAATCGACCGGAGCGACAGGAAAATCAAAGTCCAACGTGGGTTCTTAACAATTTACAAATCGAAAGACGAGGAATCAAAATTCAACAAAACCAGCGCGTCAGAGCAGGTAATGGAGGAGCTACATAGACTAATCGATTTCTTCACACAAAAGGGCGACAGAGAAATCAGCCTAACAATTGTCGGACACAGCCTAGGCGGCGCGCTGTCGCTTCTGACAGCCTACGAGGCAGGGGCATCATTTCCCGACATCCACGTGAGCGTAATGTCATTCGGAGCGCCAAGAGTGGGGAATTTAGCATTCAGGGAGAAATTAAACGAGATGGGTGTGAAGACACTCCGCGTAGTGATACGACAGGACATTGTCCCCAAGCTTCCGGGGCTGTTCGTGAATTCGATTGTGAACAAGCTGAGCGCAGTGACAGGGAAGCTGAATTGGCTGTACAGACATGTGGGGACGCAGCTGAAAATGGACATGTACATGTCGCCGTACTTGAAGAGGGAGTCGGACATGTCGGGGAGCCATAATTTGGAGATATATTTGCATTTGGTGGATGGGTTTGTGAGTAGGAGGGGGAAGTTCAGGTGGAATTCGAGGAGGGATGTGGCGTTGGTGAACAAAGGGAGCGATATGCTTGTGGAAGAGCTGAGGATTCCGGAGTTTTGGTATCAGTTGCCGCATAAGGGATTGGTGAAGAACAGGTTTGGGAGATGGGTTAAGCCTGGGAGGAACGCTGAGGATATTCCTTCTCCCTTCTCTCGTCCTTCCAATATTTGA

mRNA sequence

ATGGAGGGGAGAATCGTGACGCCGAGAGGAGTCCCACTGAAAAGAGTAACGATGTTAAGAAACGGAACGAAGAAGACGAAATGGTATTGGAAATTGAAATGGGGGATCCGATTAAGAGCCATAAAAAATGCTCTCTCTTCTTCCTTACACAACCAGCGCCGCCGTTTGACATGTGCTACCGCCACCGGCCAACTAACTCCCGTCAACGGTGCAGCGTCGCCGCTTGTAATTCAGCCATCGAAATCAACCTCTAAGAAAAACGACCTCCGTTTGGCAAAGCCACTCGCCTCGCTCCTCCGTATGCCACAACGCGCCGCTGACTTCGTCGACTACGGCAACCACATGACCCCAACATTCTCCCCTCGTGATAAAATCGCCGATGTATGGCGTGACCTCCACGGCGGTTCTAATTGGGAAGGTCTACTAGATCCCCTCCACCCCTTCCTCCGCCGCGAGCTTGTTAAATACGGCGAGTTCGCTCAAGCCACCTACGACGCTTTTGACTTCGACCCACTTTCTGAATTCCGCGGCAGCTGTAGATACAACCGCCATAAGCTCCTCCACGAATTGGGTCTGGCTCAAAACGGCTACAAAGTGACTAGATACATCTACGCCTTGTCCCCTGTTGACGGTCCGGATTGGTTCGAGAGTTCCAAAATCGGCGAGGTGTGGAGCAGAGATTCGAATTGGATGGGGTTTGTAGCCGTTAGTAACGACGAGGAGAGCGAGAGAATCGGGCGGCGGGATATCCTCGTGGCCTGGAGAGGAACGGTAACCCCAACGGAATGGTACATCGATCTGAAAACCAAGCTGAAAAAAATCGACCGGAGCGACAGGAAAATCAAAGTCCAACGTGGGTTCTTAACAATTTACAAATCGAAAGACGAGGAATCAAAATTCAACAAAACCAGCGCGTCAGAGCAGGTAATGGAGGAGCTACATAGACTAATCGATTTCTTCACACAAAAGGGCGACAGAGAAATCAGCCTAACAATTGTCGGACACAGCCTAGGCGGCGCGCTGTCGCTTCTGACAGCCTACGAGGCAGGGGCATCATTTCCCGACATCCACGTGAGCGTAATGTCATTCGGAGCGCCAAGAGTGGGGAATTTAGCATTCAGGGAGAAATTAAACGAGATGGGTGTGAAGACACTCCGCGTAGTGATACGACAGGACATTGTCCCCAAGCTTCCGGGGCTGTTCGTGAATTCGATTGTGAACAAGCTGAGCGCAGTGACAGGGAAGCTGAATTGGCTGTACAGACATGTGGGGACGCAGCTGAAAATGGACATGTACATGTCGCCGTACTTGAAGAGGGAGTCGGACATGTCGGGGAGCCATAATTTGGAGATATATTTGCATTTGGTGGATGGGTTTGTGAGTAGGAGGGGGAAGTTCAGGTGGAATTCGAGGAGGGATGTGGCGTTGGTGAACAAAGGGAGCGATATGCTTGTGGAAGAGCTGAGGATTCCGGAGTTTTGGTATCAGTTGCCGCATAAGGGATTGGTGAAGAACAGGTTTGGGAGATGGGTTAAGCCTGGGAGGAACGCTGAGGATATTCCTTCTCCCTTCTCTCGTCCTTCCAATATTTGA

Coding sequence (CDS)

ATGGAGGGGAGAATCGTGACGCCGAGAGGAGTCCCACTGAAAAGAGTAACGATGTTAAGAAACGGAACGAAGAAGACGAAATGGTATTGGAAATTGAAATGGGGGATCCGATTAAGAGCCATAAAAAATGCTCTCTCTTCTTCCTTACACAACCAGCGCCGCCGTTTGACATGTGCTACCGCCACCGGCCAACTAACTCCCGTCAACGGTGCAGCGTCGCCGCTTGTAATTCAGCCATCGAAATCAACCTCTAAGAAAAACGACCTCCGTTTGGCAAAGCCACTCGCCTCGCTCCTCCGTATGCCACAACGCGCCGCTGACTTCGTCGACTACGGCAACCACATGACCCCAACATTCTCCCCTCGTGATAAAATCGCCGATGTATGGCGTGACCTCCACGGCGGTTCTAATTGGGAAGGTCTACTAGATCCCCTCCACCCCTTCCTCCGCCGCGAGCTTGTTAAATACGGCGAGTTCGCTCAAGCCACCTACGACGCTTTTGACTTCGACCCACTTTCTGAATTCCGCGGCAGCTGTAGATACAACCGCCATAAGCTCCTCCACGAATTGGGTCTGGCTCAAAACGGCTACAAAGTGACTAGATACATCTACGCCTTGTCCCCTGTTGACGGTCCGGATTGGTTCGAGAGTTCCAAAATCGGCGAGGTGTGGAGCAGAGATTCGAATTGGATGGGGTTTGTAGCCGTTAGTAACGACGAGGAGAGCGAGAGAATCGGGCGGCGGGATATCCTCGTGGCCTGGAGAGGAACGGTAACCCCAACGGAATGGTACATCGATCTGAAAACCAAGCTGAAAAAAATCGACCGGAGCGACAGGAAAATCAAAGTCCAACGTGGGTTCTTAACAATTTACAAATCGAAAGACGAGGAATCAAAATTCAACAAAACCAGCGCGTCAGAGCAGGTAATGGAGGAGCTACATAGACTAATCGATTTCTTCACACAAAAGGGCGACAGAGAAATCAGCCTAACAATTGTCGGACACAGCCTAGGCGGCGCGCTGTCGCTTCTGACAGCCTACGAGGCAGGGGCATCATTTCCCGACATCCACGTGAGCGTAATGTCATTCGGAGCGCCAAGAGTGGGGAATTTAGCATTCAGGGAGAAATTAAACGAGATGGGTGTGAAGACACTCCGCGTAGTGATACGACAGGACATTGTCCCCAAGCTTCCGGGGCTGTTCGTGAATTCGATTGTGAACAAGCTGAGCGCAGTGACAGGGAAGCTGAATTGGCTGTACAGACATGTGGGGACGCAGCTGAAAATGGACATGTACATGTCGCCGTACTTGAAGAGGGAGTCGGACATGTCGGGGAGCCATAATTTGGAGATATATTTGCATTTGGTGGATGGGTTTGTGAGTAGGAGGGGGAAGTTCAGGTGGAATTCGAGGAGGGATGTGGCGTTGGTGAACAAAGGGAGCGATATGCTTGTGGAAGAGCTGAGGATTCCGGAGTTTTGGTATCAGTTGCCGCATAAGGGATTGGTGAAGAACAGGTTTGGGAGATGGGTTAAGCCTGGGAGGAACGCTGAGGATATTCCTTCTCCCTTCTCTCGTCCTTCCAATATTTGA

Protein sequence

MEGRIVTPRGVPLKRVTMLRNGTKKTKWYWKLKWGIRLRAIKNALSSSLHNQRRRLTCATATGQLTPVNGAASPLVIQPSKSTSKKNDLRLAKPLASLLRMPQRAADFVDYGNHMTPTFSPRDKIADVWRDLHGGSNWEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFRGSCRYNRHKLLHELGLAQNGYKVTRYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSNDEESERIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRSDRKIKVQRGFLTIYKSKDEESKFNKTSASEQVMEELHRLIDFFTQKGDREISLTIVGHSLGGALSLLTAYEAGASFPDIHVSVMSFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVNKLSAVTGKLNWLYRHVGTQLKMDMYMSPYLKRESDMSGSHNLEIYLHLVDGFVSRRGKFRWNSRRDVALVNKGSDMLVEELRIPEFWYQLPHKGLVKNRFGRWVKPGRNAEDIPSPFSRPSNI
BLAST of Cp4.1LG20g05490 vs. Swiss-Prot
Match: PLA16_ARATH (Phospholipase A1-Igamma2, chloroplastic OS=Arabidopsis thaliana GN=At2g30550 PE=1 SV=2)

HSP 1 Score: 392.5 bits (1007), Expect = 7.3e-108
Identity = 197/415 (47.47%), Postives = 274/415 (66.02%), Query Frame = 1

Query: 124 KIADVWRDLHGGSNWEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFRGSCRYNR 183
           ++ D WR + G  +W GL+DP+ P LR EL++YGE AQA YDAFDFDP S++ G+ R+ R
Sbjct: 100 RLRDTWRKIQGEDDWAGLMDPMDPILRSELIRYGEMAQACYDAFDFDPASKYCGTSRFTR 159

Query: 184 HKLLHELGLAQNGYKVTRYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSNDEES- 243
            +    LG+  +GY+V RY+YA S ++ P++F  S+  +VWS+++NWMG+VAVS+DE S 
Sbjct: 160 LEFFDSLGMIDSGYEVARYLYATSNINLPNFFSKSRWSKVWSKNANWMGYVAVSDDETSR 219

Query: 244 ERIGRRDILVAWRGTVTPTEWYIDLKTKLK-----KIDRSDRKIKVQRGFLTIYKSKDEE 303
            R+GRRDI +AWRGTVT  EW  DLK  LK     KI   D  +KV+ GFL +Y  KD  
Sbjct: 220 NRLGRRDIAIAWRGTVTKLEWIADLKDYLKPVTENKIRCPDPAVKVESGFLDLYTDKDTT 279

Query: 304 SKFNKTSASEQVMEELHRLIDFFTQKGDREISLTIVGHSLGGALSLLTAYEAGASFPD-- 363
            KF + SA EQ++ E+ RL++      D ++S+T+ GHSLGGAL++L+AY+      +  
Sbjct: 280 CKFARFSAREQILTEVKRLVEEHGDDDDSDLSITVTGHSLGGALAILSAYDIAEMRLNRS 339

Query: 364 -----IHVSVMSFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVN-SIVNKL 423
                I V+V+++G PRVGN+ FRE++ E+GVK +RVV   D+VPK PGLF+N S  + L
Sbjct: 340 KKGKVIPVTVLTYGGPRVGNVRFRERMEELGVKVMRVVNVHDVVPKSPGLFLNESRPHAL 399

Query: 424 SAVTGKLNWLYRHVGTQLKMDMYMSPYLKRESDMSGSHNLEIYLHLVDGFVSRRGKFRWN 483
             +   L W Y HVG +L +D   SP+LK   D+S +HNLE  LHL+DG+  +  +F  +
Sbjct: 400 MKIAEGLPWCYSHVGEELALDHQNSPFLKPSVDVSTAHNLEAMLHLLDGYHGKGERFVLS 459

Query: 484 SRRDVALVNKGSDMLVEELRIPEFWYQLPHKGLVKNRFGRWVKPGR-NAEDIPSP 524
           S RD ALVNK SD L E L+IP FW Q  +KG+V+N  GRW++  R   ED  SP
Sbjct: 460 SGRDHALVNKASDFLKEHLQIPPFWRQDANKGMVRNSEGRWIQAERLRFEDHHSP 514

BLAST of Cp4.1LG20g05490 vs. Swiss-Prot
Match: PLA15_ARATH (Phospholipase A1-Igamma1, chloroplastic OS=Arabidopsis thaliana GN=At1g06800 PE=1 SV=2)

HSP 1 Score: 390.6 bits (1002), Expect = 2.8e-107
Identity = 201/474 (42.41%), Postives = 289/474 (60.97%), Query Frame = 1

Query: 73  SPLVIQPSKSTSKKNDLRLAKPLASLLRMPQRAADFVDYGNHMTPTFSPRD-------KI 132
           S L+  P++S+  +   R  +  +   R+ Q +     YG         RD       ++
Sbjct: 31  STLITFPARSSPARAMSRTDEEASISTRLEQES-----YGLTTAEDIRRRDGEAKESKRL 90

Query: 133 ADVWRDLHGGSNWEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFRGSCRYNRHK 192
            D WR + G  +W GL+DP+ P LR EL++YGE AQA YDAFDFDP S + GSCR+ R  
Sbjct: 91  RDTWRKIQGEDDWAGLMDPMDPVLRSELIRYGEMAQACYDAFDFDPFSRYCGSCRFTRRH 150

Query: 193 LLHELGLAQNGYKVTRYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSNDEESE-- 252
           L   LG+  +GY+V RY+YA S ++ P++F  S+  +VWS+++NWMG+VAVS+D E+   
Sbjct: 151 LFDSLGIIDSGYEVARYLYATSNINLPNFFSKSRWSKVWSKNANWMGYVAVSDDNEATRC 210

Query: 253 RIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRS-----DRKIKVQRGFLTIYKSKDEES 312
           R+GRRDI +AWRGTVT  EW  DLK  LK +  +     D  +K + GFL +Y  KD   
Sbjct: 211 RLGRRDIAIAWRGTVTRLEWIADLKDFLKPVSGNGFRCPDPAVKAESGFLDLYTDKDTSC 270

Query: 313 KFNKTSASEQVMEELHRLIDFFTQKGDREISLTIVGHSLGGALSLLTAYEAGASFPD--- 372
            F+K SA EQV+ E+ RL++ +  +   E+S+T+ GHSLGGAL++L+AY+      +   
Sbjct: 271 NFSKFSAREQVLTEVKRLVERYGDEEGEELSITVTGHSLGGALAVLSAYDVAEMGVNRTR 330

Query: 373 ----IHVSVMSFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNS-IVNKLS 432
               I V+  ++G PRVGN+ F+E++ ++GVK LRVV   D+V K PGLF+N      L 
Sbjct: 331 KGKVIPVTAFTYGGPRVGNIRFKERIEKLGVKVLRVVNEHDVVAKSPGLFLNERAPQALM 390

Query: 433 AVTGKLNWLYRHVGTQLKMDMYMSPYLKRESDMSGSHNLEIYLHLVDGFVSRRGKFRWNS 492
            + G L W Y HVG  L +D   SP+LK   D+S +HNLE  LHL+DG+  +  +F  +S
Sbjct: 391 KLAGGLPWCYSHVGEMLPLDHQKSPFLKPTVDLSTAHNLEALLHLLDGYHGKGQRFVLSS 450

Query: 493 RRDVALVNKGSDMLVEELRIPEFWYQLPHKGLVKNRFGRWVKPGR-NAEDIPSP 524
            RD ALVNK SD L +   +P +W Q  +KG+V+N  GRW++P R  A+D  +P
Sbjct: 451 GRDPALVNKASDFLKDHFMVPPYWRQDANKGMVRNTDGRWIQPDRIRADDQHAP 499

BLAST of Cp4.1LG20g05490 vs. Swiss-Prot
Match: PLA17_ARATH (Phospholipase A1-Igamma3, chloroplastic OS=Arabidopsis thaliana GN=At1g51440 PE=1 SV=1)

HSP 1 Score: 361.3 bits (926), Expect = 1.8e-98
Identity = 185/409 (45.23%), Postives = 263/409 (64.30%), Query Frame = 1

Query: 125 IADVWRDLHGGSNWEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFRGSCRYNRH 184
           + ++WR++ G +NWEG LDP++  LRRE+++YGEFAQA YD+FDFDP S++ GSC+Y+  
Sbjct: 87  LREIWREVQGCNNWEGQLDPMNNHLRREIIRYGEFAQACYDSFDFDPHSKYCGSCKYHPS 146

Query: 185 KLLHELGL-AQNGYKVTRYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSNDEES- 244
                L L    GY +TRY+YA S ++ P++F+ SK+  +WS+ +NWMGFVAV+ DEE  
Sbjct: 147 DFFLNLDLHLHKGYTITRYLYATSNINLPNFFQKSKLSSIWSQHANWMGFVAVATDEEEV 206

Query: 245 ERIGRRDILVAWRGTVTPTEWYIDLKTKLKKID-RSDRKIKVQRGFLTIYKSKDEESKFN 304
            R+GRRDI++AWRGTVT  EW  DLK  L   +   D  IK++ GF  +Y  K++  KF+
Sbjct: 207 SRLGRRDIVIAWRGTVTYLEWIYDLKDILCSANFGDDPSIKIELGFHDLYTKKEDSCKFS 266

Query: 305 KTSASEQVMEELHRLIDFF-TQKGDREISLTIVGHSLGGALSLLTAYEAGA----SFPD- 364
             SA EQV+ E+ RLI+++ T++   + S+T+ GHSLG +L+L++AY+         P+ 
Sbjct: 267 SFSAREQVLAEVKRLIEYYGTEEEGHKTSITVTGHSLGASLALVSAYDIAELNLNHVPEN 326

Query: 365 ---IHVSVMSFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVNKLSAV 424
              I ++V SF  PRVGNL F+E+ +E+GVK LRVV   D VP +PG+F N        V
Sbjct: 327 NYKIPITVFSFSGPRVGNLRFKERCDELGVKVLRVVNVHDKVPSVPGIFTNEKFQFQKYV 386

Query: 425 TGKLN--WLYRHVGTQLKMDMYMSPYLKRESDMSGSHNLEIYLHLVDGFVSR----RGKF 484
             K +  W Y HVG +L +D   SP+LK   D+  +HNLE  LHLVDG+  +      +F
Sbjct: 387 EEKTSFPWSYAHVGVELALDHKKSPFLKPTKDLGCAHNLEALLHLVDGYHGKDEEAEKRF 446

Query: 485 RWNSRRDVALVNKGSDMLVEELRIPEFWYQLPHKGLVKNRFGRWVKPGR 516
              ++RD+ALVNK  D L  E  +P  W Q  +KG+VKN  G+WV P R
Sbjct: 447 CLVTKRDIALVNKSCDFLRGEYHVPPCWRQDENKGMVKNGDGQWVLPDR 495

BLAST of Cp4.1LG20g05490 vs. Swiss-Prot
Match: PLA4_ORYSJ (Phospholipase A1-II 4 OS=Oryza sativa subsp. japonica GN=Os01g0652300 PE=2 SV=2)

HSP 1 Score: 267.7 bits (683), Expect = 2.7e-70
Identity = 163/409 (39.85%), Postives = 228/409 (55.75%), Query Frame = 1

Query: 118 TFSPRDKIADVWRDLHGGSNWEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFRG 177
           T +PR  +A+ WR+LHG  +W+GLLDPL   LRR ++ YGE AQAT DAF  +  S   G
Sbjct: 3   TTAPR-AVAERWRELHGEDHWKGLLDPLDADLRRSVIGYGELAQATNDAFIREAWSPHAG 62

Query: 178 SCRYNRHKLLHELGLAQNG---YKVTRYIYALSPVDG-PDWFESSKIGEVWSRDSNWMGF 237
           +CRY+R + L +   +      Y+VT + YA +   G P  F       V +R+SNWMG+
Sbjct: 63  ACRYSRDRFLEKAQASTQLAGLYEVTAFFYATAGAGGVPAPFM------VRNRESNWMGY 122

Query: 238 VAVSNDEESERIGRRDILVAWRGTVTPTEWYIDLKTKLKKI-------DRSDRKIKVQRG 297
           VAV+ D     +GRRD++VAWRGTV P EW  DL   L           RS    +V RG
Sbjct: 123 VAVATDAGVAALGRRDVVVAWRGTVRPMEWLNDLDFTLVSAAGVLGAGGRSPAP-RVHRG 182

Query: 298 FLTIYKSKDEESKFNKTSASEQVMEELHRLIDFFTQKGDREISLTIVGHSLGGALSLLTA 357
           +L+IY + D  SK++K SA EQ+ +E+ RL+D +    D E S+T+VGHSLG A++ L A
Sbjct: 183 WLSIYTASDPASKYSKLSAREQISDEIKRLMDKYK---DEETSITVVGHSLGAAVATLNA 242

Query: 358 YEAGASFPDIH----VSVMSFGAPRVGNLAFREKLNEM-GVKTLRVVIRQDIVPKLPGLF 417
            +  ++  + H    V+ ++F  PRVG+  FR+  +E+ G++ LRV    D+VPK P + 
Sbjct: 243 ADIVSNGLNQHGACPVTAVAFACPRVGDSGFRKLFDELPGLRLLRVCNSPDVVPKYPPMG 302

Query: 418 VNSIVNKLSAVTGKLNWLYRHVGTQLKMDMYMSPYLKRESDMSGSHNLEIYLHLVDGFVS 477
                             Y  VG +L +D   SPYLK   + +  H+LE Y+H V G   
Sbjct: 303 ------------------YADVGVELPVDTRRSPYLKSPGNQAVWHSLECYMHGVAGAQG 362

Query: 478 RRGKFRWNSRRDVALVNKGSDMLVEELRIPEFWYQLPHKGLVKNRFGRW 511
           +RG F+    RDVALVNK  D L EE  +P  W     KG+V+   G W
Sbjct: 363 KRGGFKLEVDRDVALVNKNVDALKEEYHVPPSWSVQRDKGMVRGADGHW 382

BLAST of Cp4.1LG20g05490 vs. Swiss-Prot
Match: PLA14_ARATH (Phospholipase A1-Ibeta2, chloroplastic OS=Arabidopsis thaliana GN=At4g16820 PE=1 SV=2)

HSP 1 Score: 262.3 bits (669), Expect = 1.1e-68
Identity = 152/376 (40.43%), Postives = 220/376 (58.51%), Query Frame = 1

Query: 120 SPRDKIADVWRDLHGGSNWEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFRGSC 179
           SP+ K+   WR+LHG +NW GLLDPL   LRRELV+YGEF QA Y AF  DP     GS 
Sbjct: 122 SPKSKLGSKWRELHGLNNWAGLLDPLDENLRRELVRYGEFVQAAYHAFHSDP----EGSP 181

Query: 180 RYNRHKLLHELGLAQNGYKVTRYIYALSPVDGPDWFESSKIGEVW-SRDSNWMGFVAVSN 239
           R+        + L    +KVT+ +YA S V  P W +       W ++ ++W+G+VAV +
Sbjct: 182 RH--------VALPDGSFKVTKSLYATSSVRLPKWIDDVAPDLRWMTKQTSWVGYVAVCD 241

Query: 240 D-EESERIGRRDILVAWRGTVTPTEWYIDLKTKL-----KKIDRSD-RKIKVQRGFLTIY 299
           D  E  R+GRR+I++A RGT T  EW  + +  L      K D+SD  + KV+ GF ++Y
Sbjct: 242 DPREIRRMGRREIVIALRGTATLLEWSENFRPNLVSMPEPKPDQSDPTRPKVECGFNSLY 301

Query: 300 KSKDEESKFNKTSASEQVMEELHRLIDFFTQKGDREISLTIVGHSLGGALSLLTAYEAGA 359
            + D+ +     S +E ++ E+ RL++ +      E+S+++ GHSLG A++LL A +   
Sbjct: 302 TTGDQHA----PSLAESLVGEISRLVELY---AGEELSISVTGHSLGAAIALLAADDIAE 361

Query: 360 SFPDI-HVSVMSFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVNKLS 419
             P    V+V SFG PRVGN  F ++L+  GVK LRVV  QD+V K+PG+F ++     S
Sbjct: 362 RVPHAPPVAVFSFGGPRVGNREFADRLDSKGVKVLRVVNSQDVVTKVPGIFADNDKQGQS 421

Query: 420 AVTGKL------------NWLYRHVGTQLKMDMYMSPYLKRESDMSGSHNLEIYLHLVDG 475
              G+              W Y HVG +L++DM MSPYLK  +D++  H+LE YLHLVDG
Sbjct: 422 RNNGRSPGGIMEMVERNNPWAYSHVGAELRVDMKMSPYLKPNADVACCHDLEAYLHLVDG 478

BLAST of Cp4.1LG20g05490 vs. TrEMBL
Match: A0A0A0M111_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G537320 PE=4 SV=1)

HSP 1 Score: 958.0 bits (2475), Expect = 4.8e-276
Identity = 464/535 (86.73%), Postives = 501/535 (93.64%), Query Frame = 1

Query: 1   MEGRIVTPRGVPLKRVTMLRNGTKKTKWYWKLKWGIRLRAIKNALSSSLHNQRRRLTCAT 60
           ME +  TPRG+P+KR TMLRNGTKKTKWYWKLK GI+ +AIK ALSSSLHNQRRRLTC+T
Sbjct: 1   MEPKFATPRGIPVKRTTMLRNGTKKTKWYWKLKLGIQFKAIKAALSSSLHNQRRRLTCST 60

Query: 61  A----TGQLTPVNGAASPLVIQPSKSTSKKNDLRLAKPLASLLRMPQRAADFVDYGNHMT 120
           A    T Q TP NG  SPLVI  SKS SKKNDLRLAKPLASLLRMP RA+DF+D+GNHMT
Sbjct: 61  AITTTTPQHTPTNGLVSPLVIHQSKS-SKKNDLRLAKPLASLLRMPLRASDFIDFGNHMT 120

Query: 121 PTFSPRDKIADVWRDLHGGSNWEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFR 180
           PT SPRDKI+ VWRDLHG S+WEGLLDPLHPFLRRE+VKYGEFAQATYDAFDFDPLSEF 
Sbjct: 121 PTLSPRDKISAVWRDLHGASDWEGLLDPLHPFLRREIVKYGEFAQATYDAFDFDPLSEFC 180

Query: 181 GSCRYNRHKLLHELGLAQNGYKVTRYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAV 240
           GSCRYNR KLL+ELGLAQNGYKVT+YIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAV
Sbjct: 181 GSCRYNRQKLLNELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAV 240

Query: 241 SNDEESERIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRSDRKIKVQRGFLTIYKSKDE 300
           S+DEES+RIGRRDILV+WRGTVTPTEWYIDLKTKLKKIDRS++K+KVQRGFLTIYKSKDE
Sbjct: 241 SSDEESDRIGRRDILVSWRGTVTPTEWYIDLKTKLKKIDRSNKKVKVQRGFLTIYKSKDE 300

Query: 301 ESKFNKTSASEQVMEELHRLIDFFTQKGDREISLTIVGHSLGGALSLLTAYEAGASFP-D 360
           +SKFNKTSASEQVMEELHRLIDFF QKGDREISLTI GHSLGGALSLLTAYEAG +FP D
Sbjct: 301 DSKFNKTSASEQVMEELHRLIDFFKQKGDREISLTITGHSLGGALSLLTAYEAGVTFPAD 360

Query: 361 IHVSVMSFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVNKLSAVTGK 420
           +HVSV+SFGAPRVGNLAFREKLNEMGVK LRVVI QDIVPKLPGLFVNSIVNKLSAVTGK
Sbjct: 361 VHVSVVSFGAPRVGNLAFREKLNEMGVKILRVVIGQDIVPKLPGLFVNSIVNKLSAVTGK 420

Query: 421 LNWLYRHVGTQLKMDMYMSPYLKRESDMSGSHNLEIYLHLVDGFVSRRGKFRWNSRRDVA 480
           LNW+YRHVG +L+M+M+MSPYLK++SDMSGSHNLEIYLHLVDGFV+++GKFRWNSRRDVA
Sbjct: 421 LNWVYRHVGKELRMNMFMSPYLKKDSDMSGSHNLEIYLHLVDGFVTKKGKFRWNSRRDVA 480

Query: 481 LVNKGSDMLVEELRIPEFWYQLPHKGLVKNRFGRWVKPGRNAEDIPSPFSRPSNI 531
           LVNKGSDMLVEELRIPEFWYQLPHKGL+KNRFGRWVKPGRN EDIPSPFS+PS +
Sbjct: 481 LVNKGSDMLVEELRIPEFWYQLPHKGLIKNRFGRWVKPGRNVEDIPSPFSQPSQV 534

BLAST of Cp4.1LG20g05490 vs. TrEMBL
Match: B9HNS4_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0009s06300g PE=4 SV=2)

HSP 1 Score: 662.9 bits (1709), Expect = 3.2e-187
Identity = 320/518 (61.78%), Postives = 408/518 (78.76%), Query Frame = 1

Query: 12  PLKRVTMLRNGTKKTKWYWKLKWGIRLRAIKNALSSSLHNQRR---RLTCATATGQLTPV 71
           P ++VT     ++KT+  WKLK  I   AIK A+S ++  +      L+CA +  +L+  
Sbjct: 5   PFRQVTTEETRSRKTRRAWKLKLNITWHAIKKAVSYNVKKRLHLHLHLSCARSMRRLS-T 64

Query: 72  NGAASPLVIQPSKSTSKKNDLRL-AKPLASLLRMPQRAADFVDYGNHMTPTFSPRDKIAD 131
           N     L ++P +++  K   R  +K LASLL +P  A DFVD G+HMTPT SP+  I+ 
Sbjct: 65  NQTHQELAVKPHQASKPKQKARNPSKTLASLLHVPYTALDFVDRGDHMTPTLSPKQSISA 124

Query: 132 VWRDLHGGSNWEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFRGSCRYNRHKLL 191
           VW+++HG SNWE LLDP HP LRRE++KYGEFAQ TYDAFDFDPLS+F GSCRYNR K  
Sbjct: 125 VWKEIHGSSNWETLLDPFHPSLRREILKYGEFAQGTYDAFDFDPLSDFCGSCRYNRRKFF 184

Query: 192 HELGLAQNGYKVTRYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSNDEESERIGR 251
             LGL ++GYKV +YIYALS VD P+W + S     WS+DSNWMG+VAVS  EES+RIGR
Sbjct: 185 ETLGLTKHGYKVKKYIYALSHVDVPEWLKRSYA--TWSKDSNWMGYVAVSRREESQRIGR 244

Query: 252 RDILVAWRGTVTPTEWYIDLKTKLKKIDRSDRKIKVQRGFLTIYKSKDEESKFNKTSASE 311
           RDI+VAWRGTV+P+EW+ DL T L+ ID ++  +KVQ GFL++YKSKDE +++NK SASE
Sbjct: 245 RDIMVAWRGTVSPSEWFKDLTTSLEHIDNTN--VKVQEGFLSVYKSKDELTRYNKLSASE 304

Query: 312 QVMEELHRLIDFFTQKGDREISLTIVGHSLGGALSLLTAYEAGASFPDIHVSVMSFGAPR 371
           QVM+E+ RL++F+  KG+ E+SLT+ GHSLGGAL+LL AYEA  + PD+ VSV+SFGAPR
Sbjct: 305 QVMQEVMRLVNFYRGKGE-EVSLTVTGHSLGGALALLNAYEAATAIPDLFVSVISFGAPR 364

Query: 372 VGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVNKLSAVTGKLNWLYRHVGTQL 431
           VGN+AF+EKLNE+GVKTLRVV++QDIVPKLPGL +N ++NK   +TGKLNW+YRHVGTQL
Sbjct: 365 VGNIAFKEKLNELGVKTLRVVVKQDIVPKLPGL-LNKMLNKFHGLTGKLNWVYRHVGTQL 424

Query: 432 KMDMYMSPYLKRESDMSGSHNLEIYLHLVDGFVSRRGKFRWNSRRDVALVNKGSDMLVEE 491
           K+D +MSPYLK ESD+SGSHNLE+YLHL+DGF S++ K+RWN+RRD+ALVNKGSDML+E+
Sbjct: 425 KLDAFMSPYLKPESDLSGSHNLELYLHLIDGFFSKKSKYRWNARRDLALVNKGSDMLIED 484

Query: 492 LRIPEFWYQLPHKGLVKNRFGRWVKPGRNAEDIPSPFS 526
           L+IPEFWYQ P+KGLV N++GRWVKPGR  EDIPSP S
Sbjct: 485 LKIPEFWYQFPYKGLVLNQYGRWVKPGRLPEDIPSPLS 515

BLAST of Cp4.1LG20g05490 vs. TrEMBL
Match: A0A067L0J4_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_04663 PE=4 SV=1)

HSP 1 Score: 661.8 bits (1706), Expect = 7.2e-187
Identity = 317/515 (61.55%), Postives = 409/515 (79.42%), Query Frame = 1

Query: 12  PLKR-VTMLRNGTKKTKWYWKLKWGIRLRAIKNALSSSLHNQRRRLTCATATGQLTPVNG 71
           PLKR V   +  ++KT+  WKLK  I  +AIK A +S   N+  RL+C     QL+ +N 
Sbjct: 5   PLKRRVLAEKTRSRKTRRVWKLKLNITWQAIKKAFTSK--NRLLRLSCV----QLSTIN- 64

Query: 72  AASPLVIQPSKSTSKKNDLRLAKPLASLLRMPQRAADFVDYGNHMTPTFSPRDKIADVWR 131
                + Q +K   +KN  +  K LA+LL +P  A+DF+D G+ MTPT SP++ ++  WR
Sbjct: 65  -----IHQDAKK--RKNSSKPHKTLANLLHVPYTASDFIDRGDQMTPTLSPKENVSAKWR 124

Query: 132 DLHGGSNWEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFRGSCRYNRHKLLHEL 191
           ++HG  NWE LLDPL P LRRE++KYGEFA ATYDAFDFDPLSE+ GSCRYNRHK+  EL
Sbjct: 125 EIHGSCNWENLLDPLDPCLRREILKYGEFATATYDAFDFDPLSEYCGSCRYNRHKIFEEL 184

Query: 192 GLAQNGYKVTRYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSNDEESERIGRRDI 251
           GL ++ Y VT+YIYA+S VD P+WF  +    +WS+DSNWMG+VAVSND+ES+R+GRRDI
Sbjct: 185 GLNKHNYNVTKYIYAMSHVDVPEWFVRAH--SIWSKDSNWMGYVAVSNDQESKRLGRRDI 244

Query: 252 LVAWRGTVTPTEWYIDLKTKLKKIDRSDRKIKVQRGFLTIYKSKDEESKFNKTSASEQVM 311
           +VAWRGTV PTEW+ DLKTKL++ID  + KIKVQ GF +IYKSK E +++NK SASEQVM
Sbjct: 245 VVAWRGTVAPTEWFTDLKTKLEEIDEENEKIKVQYGFYSIYKSKSEFTRYNKLSASEQVM 304

Query: 312 EELHRLIDFFTQKGDREISLTIVGHSLGGALSLLTAYEAGASFPDIHVSVMSFGAPRVGN 371
           +E+ +L++ + + G+ E+SLT+ GHSLGGAL++L AYEA  S P++ VSV+SFGAPRVGN
Sbjct: 305 QEIRKLVNCYRENGE-EVSLTVTGHSLGGALAVLNAYEAANSIPNLFVSVISFGAPRVGN 364

Query: 372 LAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVNKLSAVTGKLNWLYRHVGTQLKMD 431
           LAF+EKLNE+GVKTLRVV++QDIVPK+PG+ VN I+ K++ +T +LNW+YRHVGTQLK+D
Sbjct: 365 LAFKEKLNELGVKTLRVVVKQDIVPKVPGIIVNKILRKINTITQRLNWVYRHVGTQLKLD 424

Query: 432 MYMSPYLKRESDMSGSHNLEIYLHLVDGFVSRRGKFRWNSRRDVALVNKGSDMLVEELRI 491
           M+MSPYLK  SD SGSHNLE+YLHL+DGF+ ++ +FRW++RRD+ALVNK SDML+EEL+I
Sbjct: 425 MFMSPYLKHVSDFSGSHNLEVYLHLLDGFLGKKLQFRWHARRDLALVNKSSDMLIEELKI 484

Query: 492 PEFWYQLPHKGLVKNRFGRWVKPGRNAEDIPSPFS 526
           PEFWYQLPHKGLV N++GRWVKP R  EDIPSPFS
Sbjct: 485 PEFWYQLPHKGLVLNKYGRWVKPTREPEDIPSPFS 502

BLAST of Cp4.1LG20g05490 vs. TrEMBL
Match: B9SYI5_RICCO (Triacylglycerol lipase, putative OS=Ricinus communis GN=RCOM_1288510 PE=4 SV=1)

HSP 1 Score: 660.6 bits (1703), Expect = 1.6e-186
Identity = 320/519 (61.66%), Postives = 405/519 (78.03%), Query Frame = 1

Query: 12  PLKR-VTMLRNGTKKTKWYWKLKWGIRLRAIKNALSSSLHNQRRRLTCATATGQLTPVNG 71
           PL++ V   +  ++K +  WKLK  +  RAIK A +S     R RL+C     QL+    
Sbjct: 5   PLRQPVATKKTMSRKARRIWKLKLTVTWRAIKKAFTSK---HRLRLSCTGNMKQLSTFK- 64

Query: 72  AASPLVIQPSKSTSKKNDLRLAKPLASLLRMPQRAADFVDYGNHMTPTFSPRDKIADVWR 131
               L  + +  T K  D    K LA LL +P+ A DF+D G+ MTPT SP++ I+  W+
Sbjct: 65  ----LHQELATKTKKNGDCHPHKTLAHLL-VPRTALDFIDRGDQMTPTLSPKENISSRWQ 124

Query: 132 DLHGGSNWEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFRGSCRYNRHKLLHEL 191
           ++HG  NWE LLDPLHP+LRRE+VKYGEF +ATYDAFDFDPLSE+ GSC YNRHK+  EL
Sbjct: 125 EIHGSRNWENLLDPLHPWLRREVVKYGEFVEATYDAFDFDPLSEYCGSCLYNRHKIFEEL 184

Query: 192 GLAQNGYKVTRYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSNDEESERIGRRDI 251
           GL ++GY+ T+YIYA+S VD P+WF  ++    WS+DSNWMGFVAVSND+ES+RIGRRDI
Sbjct: 185 GLTKHGYRATKYIYAMSHVDVPEWF--ARTHTTWSKDSNWMGFVAVSNDQESQRIGRRDI 244

Query: 252 LVAWRGTVTPTEWYIDLKTKLKKI----DRSDRKIKVQRGFLTIYKSKDEESKFNKTSAS 311
           +VAWRGTV PTEWY DL+T L+      D     +KVQ GFL+IYKSK EE+++NK SAS
Sbjct: 245 MVAWRGTVAPTEWYNDLRTDLEYFEEDQDHKKNHVKVQEGFLSIYKSKSEETRYNKLSAS 304

Query: 312 EQVMEELHRLIDFFTQKGDREISLTIVGHSLGGALSLLTAYEAGASFPDIHVSVMSFGAP 371
           EQVM+EL +L++ + + G+ E+SLT+ GHSLGGAL+LL AYEA  S P++ +SV+SFGAP
Sbjct: 305 EQVMKELKKLVNLYRENGE-EVSLTLTGHSLGGALALLNAYEAATSIPNVFISVISFGAP 364

Query: 372 RVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVNKLSAVTGKLNWLYRHVGTQ 431
           RVGNLAF+EKLNE+GVKTLRVVI+QDIVPKLPG+ VN I+NKLS +T KLNW+YRHVGTQ
Sbjct: 365 RVGNLAFKEKLNELGVKTLRVVIKQDIVPKLPGIIVNKILNKLSKITHKLNWVYRHVGTQ 424

Query: 432 LKMDMYMSPYLKRESDMSGSHNLEIYLHLVDGFVSRRGKFRWNSRRDVALVNKGSDMLVE 491
           LK+DM++SPYLK+ESDMSGSHNLE+YLHL+DGF+ ++  +RWN+RRD+ALVNK ++ML+E
Sbjct: 425 LKLDMFISPYLKQESDMSGSHNLEVYLHLLDGFLGKKLNYRWNARRDLALVNKSTNMLIE 484

Query: 492 ELRIPEFWYQLPHKGLVKNRFGRWVKPGRNAEDIPSPFS 526
           EL+IPEFWYQLPHKGLV N++GRWVKP R AEDIPSPFS
Sbjct: 485 ELKIPEFWYQLPHKGLVLNKYGRWVKPSRVAEDIPSPFS 511

BLAST of Cp4.1LG20g05490 vs. TrEMBL
Match: A0A0D2UMJ2_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_009G152400 PE=4 SV=1)

HSP 1 Score: 642.1 bits (1655), Expect = 5.9e-181
Identity = 311/516 (60.27%), Postives = 398/516 (77.13%), Query Frame = 1

Query: 12  PLKRVTMLRNGTKKTKWYWKLKWGIRLRAIKNALSSSLHNQRRRLTCATATGQLTPVN-G 71
           P++ V   +    +TK  WKLK  +  + +K AL S+L + R  L  A+   +L+ +   
Sbjct: 8   PVRHVDRQKTERPRTKRVWKLKLSVTWQRMKKALKSTLKH-RLHLHLASNLSKLSTLKLN 67

Query: 72  AASPLVIQPSKS-TSKKNDLRLAKPLASLLRMPQRAADFVDYGNHMTPTFSPRDKIADVW 131
                +I+P K  T KK  +R   PLA L+++P  AADF+D+G+ MTPT SP + I+  W
Sbjct: 68  HHDMAIIKPIKHVTKKKKTIRPTMPLAQLIQLPYTAADFIDHGHAMTPTKSPIENISTRW 127

Query: 132 RDLHGGSNWEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFRGSCRYNRHKLLHE 191
           R+LHG  NW+GL++PLHP+LRRE+VKYGEF Q TYDAFDFDPLSEF GSCRYNRHKL  E
Sbjct: 128 RELHGLHNWDGLIEPLHPWLRREVVKYGEFVQGTYDAFDFDPLSEFCGSCRYNRHKLFEE 187

Query: 192 LGLAQNGYKVTRYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSNDEESERIGRRD 251
           LGL ++GYKVT+YIYA+S VD P+W E +     WS+DSNWMG+VAVS D E+ RIGRRD
Sbjct: 188 LGLTKHGYKVTKYIYAMSHVDVPEWLERTYC--TWSKDSNWMGYVAVSGDAETARIGRRD 247

Query: 252 ILVAWRGTVTPTEWYIDLKTKLKKIDRSDRKIKVQRGFLTIYKSKDEESKFNKTSASEQV 311
           ILVAWRGTV PTEWY DLKT+L+++ +++  IKVQRGFL+IY SK + S++NK SASEQV
Sbjct: 248 ILVAWRGTVAPTEWYTDLKTRLQRLGKTN--IKVQRGFLSIYSSKGDFSRYNKLSASEQV 307

Query: 312 MEELHRLIDFFTQKGDREISLTIVGHSLGGALSLLTAYEAGASFPDIHVSVMSFGAPRVG 371
            EE+ +L+ FF  +G+ E+SLTI GHSLGGAL+LL AY+A    P + ++V+SFGAPRVG
Sbjct: 308 KEEIQKLVSFFRDRGE-EVSLTICGHSLGGALALLNAYDAATYVPHLFINVISFGAPRVG 367

Query: 372 NLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVNKLSAVTGKLNWLYRHVGTQLKM 431
           N+ F+EKL E+GVKTLRVV +QDIVPKLPG  +N+I+NK +AVTG+L W+YRHVGTQLK+
Sbjct: 368 NIHFKEKLKELGVKTLRVVAKQDIVPKLPGFILNTILNKFTAVTGRLKWIYRHVGTQLKL 427

Query: 432 DMYMSPYLKRESDMSGSHNLEIYLHLVDGFVSRRGKFRWNSRRDVALVNKGSDMLVEELR 491
           D+ MSPYL R+ D +GSHNLE YLHL+DG++S+  KFRWN+RRDVALVNK +DML++EL+
Sbjct: 428 DVLMSPYLTRDPDYTGSHNLETYLHLLDGYISKTSKFRWNARRDVALVNKSTDMLIKELK 487

Query: 492 IPEFWYQLPHKGLVKNRFGRWVKPGRNAEDIPSPFS 526
           IPEFWYQ P KGLV N++GRWVKPGR  E IPSP S
Sbjct: 488 IPEFWYQRPFKGLVLNQYGRWVKPGRPVEHIPSPRS 517

BLAST of Cp4.1LG20g05490 vs. TAIR10
Match: AT1G30370.1 (AT1G30370.1 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 585.5 bits (1508), Expect = 3.3e-167
Identity = 291/513 (56.73%), Postives = 385/513 (75.05%), Query Frame = 1

Query: 24  KKTKWYWKLKWGIRL--RAIKNALSSSLH---NQRRRLTCATATGQLTPVNGAASPLVIQ 83
           ++TK  W+LK  ++L  ++IK  + S L    + ++ L    +  +   ++  A  +   
Sbjct: 16  RRTKRVWRLKQKLKLAWKSIKIRVKSHLPGFLSTKKHLFHIKSRKEEQDLSQVAQRICKI 75

Query: 84  PSKSTSKKNDLRLAKPLASLLRMPQRAAD-FVDYGNHMTPTFSPRDKIADVWRDLHGGSN 143
            + ST         K LA LL++P+ +AD F+D G+ MTP  SPR+KI+ +WR+LHG +N
Sbjct: 76  SNDST---------KSLAFLLQLPKYSADDFLDRGDLMTPAASPREKISKMWRELHGSNN 135

Query: 144 WEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFRGSCRYNRHKLLHELGLAQNGY 203
           WE LLDPLHP+LRRE+ KYGEF ++ YD+ DFDPLSEF GS RYNR+KL  ELGL ++GY
Sbjct: 136 WENLLDPLHPWLRREVTKYGEFVESVYDSLDFDPLSEFCGSSRYNRNKLFEELGLTRHGY 195

Query: 204 KVTRYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSNDEESERIGRRDILVAWRGT 263
           KVT+YIYA+S VD P WF SS +GE WS+DSNWMGFVAVS D ES RIGRRDI+VAWRGT
Sbjct: 196 KVTKYIYAMSRVDVPQWFLSSALGETWSKDSNWMGFVAVSGDRESLRIGRRDIVVAWRGT 255

Query: 264 VTPTEWYIDLKTKLKKIDRSDRK----IKVQRGFLTIYKSKDEESKFNKTSASEQVMEEL 323
           VTPTEW++DL+T ++  D   +     +KVQ GFL+IY SK E +++NK SASEQ M+E+
Sbjct: 256 VTPTEWFMDLRTSMEPFDCEGKHGKTVVKVQSGFLSIYNSKSELTRYNKESASEQTMDEV 315

Query: 324 HRLIDFFTQKGDREISLTIVGHSLGGALSLLTAYEAGASFPDI--HVSVMSFGAPRVGNL 383
            RL++FF  +G+ E+SLTI GHSLGGAL+L+ AYEA    P +  ++SV+SFGAPRVGNL
Sbjct: 316 KRLVNFFKDRGE-EVSLTITGHSLGGALALMNAYEAARDVPALSGNISVISFGAPRVGNL 375

Query: 384 AFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVNKLSAVTGKLNWLYRHVGTQLKMDM 443
           AF+EKLN +GVK LRVV +QDIVPKLPG+  N ++NKL+ +T +LNW+YRHVGTQLK+D+
Sbjct: 376 AFKEKLNSLGVKVLRVVNKQDIVPKLPGIVFNKVLNKLNPITSRLNWVYRHVGTQLKLDV 435

Query: 444 YMSPYLKRESDMSGSHNLEIYLHLVDGFVSRRGKFRWNSRRDVALVNKGSDMLVEELRIP 503
           + SPY+KR+SD+  +HNLE+YLH++DGF  ++  FR N+RRDVA VNK +DML++ LRIP
Sbjct: 436 FSSPYVKRDSDLGRAHNLEVYLHVLDGFHRKKSGFRVNARRDVASVNKSTDMLLDHLRIP 495

Query: 504 EFWYQLPHKGLVKNR-FGRWVKPGRNAEDIPSP 524
           EFWYQ+ HKGL+ N+  GRWVKP R  EDIPSP
Sbjct: 496 EFWYQVAHKGLILNKQTGRWVKPVRAPEDIPSP 518

BLAST of Cp4.1LG20g05490 vs. TAIR10
Match: AT2G30550.2 (AT2G30550.2 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 392.5 bits (1007), Expect = 4.1e-109
Identity = 197/415 (47.47%), Postives = 274/415 (66.02%), Query Frame = 1

Query: 124 KIADVWRDLHGGSNWEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFRGSCRYNR 183
           ++ D WR + G  +W GL+DP+ P LR EL++YGE AQA YDAFDFDP S++ G+ R+ R
Sbjct: 100 RLRDTWRKIQGEDDWAGLMDPMDPILRSELIRYGEMAQACYDAFDFDPASKYCGTSRFTR 159

Query: 184 HKLLHELGLAQNGYKVTRYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSNDEES- 243
            +    LG+  +GY+V RY+YA S ++ P++F  S+  +VWS+++NWMG+VAVS+DE S 
Sbjct: 160 LEFFDSLGMIDSGYEVARYLYATSNINLPNFFSKSRWSKVWSKNANWMGYVAVSDDETSR 219

Query: 244 ERIGRRDILVAWRGTVTPTEWYIDLKTKLK-----KIDRSDRKIKVQRGFLTIYKSKDEE 303
            R+GRRDI +AWRGTVT  EW  DLK  LK     KI   D  +KV+ GFL +Y  KD  
Sbjct: 220 NRLGRRDIAIAWRGTVTKLEWIADLKDYLKPVTENKIRCPDPAVKVESGFLDLYTDKDTT 279

Query: 304 SKFNKTSASEQVMEELHRLIDFFTQKGDREISLTIVGHSLGGALSLLTAYEAGASFPD-- 363
            KF + SA EQ++ E+ RL++      D ++S+T+ GHSLGGAL++L+AY+      +  
Sbjct: 280 CKFARFSAREQILTEVKRLVEEHGDDDDSDLSITVTGHSLGGALAILSAYDIAEMRLNRS 339

Query: 364 -----IHVSVMSFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVN-SIVNKL 423
                I V+V+++G PRVGN+ FRE++ E+GVK +RVV   D+VPK PGLF+N S  + L
Sbjct: 340 KKGKVIPVTVLTYGGPRVGNVRFRERMEELGVKVMRVVNVHDVVPKSPGLFLNESRPHAL 399

Query: 424 SAVTGKLNWLYRHVGTQLKMDMYMSPYLKRESDMSGSHNLEIYLHLVDGFVSRRGKFRWN 483
             +   L W Y HVG +L +D   SP+LK   D+S +HNLE  LHL+DG+  +  +F  +
Sbjct: 400 MKIAEGLPWCYSHVGEELALDHQNSPFLKPSVDVSTAHNLEAMLHLLDGYHGKGERFVLS 459

Query: 484 SRRDVALVNKGSDMLVEELRIPEFWYQLPHKGLVKNRFGRWVKPGR-NAEDIPSP 524
           S RD ALVNK SD L E L+IP FW Q  +KG+V+N  GRW++  R   ED  SP
Sbjct: 460 SGRDHALVNKASDFLKEHLQIPPFWRQDANKGMVRNSEGRWIQAERLRFEDHHSP 514

BLAST of Cp4.1LG20g05490 vs. TAIR10
Match: AT1G06800.1 (AT1G06800.1 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 390.6 bits (1002), Expect = 1.6e-108
Identity = 201/474 (42.41%), Postives = 289/474 (60.97%), Query Frame = 1

Query: 73  SPLVIQPSKSTSKKNDLRLAKPLASLLRMPQRAADFVDYGNHMTPTFSPRD-------KI 132
           S L+  P++S+  +   R  +  +   R+ Q +     YG         RD       ++
Sbjct: 31  STLITFPARSSPARAMSRTDEEASISTRLEQES-----YGLTTAEDIRRRDGEAKESKRL 90

Query: 133 ADVWRDLHGGSNWEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFRGSCRYNRHK 192
            D WR + G  +W GL+DP+ P LR EL++YGE AQA YDAFDFDP S + GSCR+ R  
Sbjct: 91  RDTWRKIQGEDDWAGLMDPMDPVLRSELIRYGEMAQACYDAFDFDPFSRYCGSCRFTRRH 150

Query: 193 LLHELGLAQNGYKVTRYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSNDEESE-- 252
           L   LG+  +GY+V RY+YA S ++ P++F  S+  +VWS+++NWMG+VAVS+D E+   
Sbjct: 151 LFDSLGIIDSGYEVARYLYATSNINLPNFFSKSRWSKVWSKNANWMGYVAVSDDNEATRC 210

Query: 253 RIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRS-----DRKIKVQRGFLTIYKSKDEES 312
           R+GRRDI +AWRGTVT  EW  DLK  LK +  +     D  +K + GFL +Y  KD   
Sbjct: 211 RLGRRDIAIAWRGTVTRLEWIADLKDFLKPVSGNGFRCPDPAVKAESGFLDLYTDKDTSC 270

Query: 313 KFNKTSASEQVMEELHRLIDFFTQKGDREISLTIVGHSLGGALSLLTAYEAGASFPD--- 372
            F+K SA EQV+ E+ RL++ +  +   E+S+T+ GHSLGGAL++L+AY+      +   
Sbjct: 271 NFSKFSAREQVLTEVKRLVERYGDEEGEELSITVTGHSLGGALAVLSAYDVAEMGVNRTR 330

Query: 373 ----IHVSVMSFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNS-IVNKLS 432
               I V+  ++G PRVGN+ F+E++ ++GVK LRVV   D+V K PGLF+N      L 
Sbjct: 331 KGKVIPVTAFTYGGPRVGNIRFKERIEKLGVKVLRVVNEHDVVAKSPGLFLNERAPQALM 390

Query: 433 AVTGKLNWLYRHVGTQLKMDMYMSPYLKRESDMSGSHNLEIYLHLVDGFVSRRGKFRWNS 492
            + G L W Y HVG  L +D   SP+LK   D+S +HNLE  LHL+DG+  +  +F  +S
Sbjct: 391 KLAGGLPWCYSHVGEMLPLDHQKSPFLKPTVDLSTAHNLEALLHLLDGYHGKGQRFVLSS 450

Query: 493 RRDVALVNKGSDMLVEELRIPEFWYQLPHKGLVKNRFGRWVKPGR-NAEDIPSP 524
            RD ALVNK SD L +   +P +W Q  +KG+V+N  GRW++P R  A+D  +P
Sbjct: 451 GRDPALVNKASDFLKDHFMVPPYWRQDANKGMVRNTDGRWIQPDRIRADDQHAP 499

BLAST of Cp4.1LG20g05490 vs. TAIR10
Match: AT1G51440.1 (AT1G51440.1 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 361.3 bits (926), Expect = 1.0e-99
Identity = 185/409 (45.23%), Postives = 263/409 (64.30%), Query Frame = 1

Query: 125 IADVWRDLHGGSNWEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFRGSCRYNRH 184
           + ++WR++ G +NWEG LDP++  LRRE+++YGEFAQA YD+FDFDP S++ GSC+Y+  
Sbjct: 87  LREIWREVQGCNNWEGQLDPMNNHLRREIIRYGEFAQACYDSFDFDPHSKYCGSCKYHPS 146

Query: 185 KLLHELGL-AQNGYKVTRYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSNDEES- 244
                L L    GY +TRY+YA S ++ P++F+ SK+  +WS+ +NWMGFVAV+ DEE  
Sbjct: 147 DFFLNLDLHLHKGYTITRYLYATSNINLPNFFQKSKLSSIWSQHANWMGFVAVATDEEEV 206

Query: 245 ERIGRRDILVAWRGTVTPTEWYIDLKTKLKKID-RSDRKIKVQRGFLTIYKSKDEESKFN 304
            R+GRRDI++AWRGTVT  EW  DLK  L   +   D  IK++ GF  +Y  K++  KF+
Sbjct: 207 SRLGRRDIVIAWRGTVTYLEWIYDLKDILCSANFGDDPSIKIELGFHDLYTKKEDSCKFS 266

Query: 305 KTSASEQVMEELHRLIDFF-TQKGDREISLTIVGHSLGGALSLLTAYEAGA----SFPD- 364
             SA EQV+ E+ RLI+++ T++   + S+T+ GHSLG +L+L++AY+         P+ 
Sbjct: 267 SFSAREQVLAEVKRLIEYYGTEEEGHKTSITVTGHSLGASLALVSAYDIAELNLNHVPEN 326

Query: 365 ---IHVSVMSFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVNKLSAV 424
              I ++V SF  PRVGNL F+E+ +E+GVK LRVV   D VP +PG+F N        V
Sbjct: 327 NYKIPITVFSFSGPRVGNLRFKERCDELGVKVLRVVNVHDKVPSVPGIFTNEKFQFQKYV 386

Query: 425 TGKLN--WLYRHVGTQLKMDMYMSPYLKRESDMSGSHNLEIYLHLVDGFVSR----RGKF 484
             K +  W Y HVG +L +D   SP+LK   D+  +HNLE  LHLVDG+  +      +F
Sbjct: 387 EEKTSFPWSYAHVGVELALDHKKSPFLKPTKDLGCAHNLEALLHLVDGYHGKDEEAEKRF 446

Query: 485 RWNSRRDVALVNKGSDMLVEELRIPEFWYQLPHKGLVKNRFGRWVKPGR 516
              ++RD+ALVNK  D L  E  +P  W Q  +KG+VKN  G+WV P R
Sbjct: 447 CLVTKRDIALVNKSCDFLRGEYHVPPCWRQDENKGMVKNGDGQWVLPDR 495

BLAST of Cp4.1LG20g05490 vs. TAIR10
Match: AT4G16820.1 (AT4G16820.1 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 262.3 bits (669), Expect = 6.4e-70
Identity = 152/376 (40.43%), Postives = 220/376 (58.51%), Query Frame = 1

Query: 120 SPRDKIADVWRDLHGGSNWEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFRGSC 179
           SP+ K+   WR+LHG +NW GLLDPL   LRRELV+YGEF QA Y AF  DP     GS 
Sbjct: 122 SPKSKLGSKWRELHGLNNWAGLLDPLDENLRRELVRYGEFVQAAYHAFHSDP----EGSP 181

Query: 180 RYNRHKLLHELGLAQNGYKVTRYIYALSPVDGPDWFESSKIGEVW-SRDSNWMGFVAVSN 239
           R+        + L    +KVT+ +YA S V  P W +       W ++ ++W+G+VAV +
Sbjct: 182 RH--------VALPDGSFKVTKSLYATSSVRLPKWIDDVAPDLRWMTKQTSWVGYVAVCD 241

Query: 240 D-EESERIGRRDILVAWRGTVTPTEWYIDLKTKL-----KKIDRSD-RKIKVQRGFLTIY 299
           D  E  R+GRR+I++A RGT T  EW  + +  L      K D+SD  + KV+ GF ++Y
Sbjct: 242 DPREIRRMGRREIVIALRGTATLLEWSENFRPNLVSMPEPKPDQSDPTRPKVECGFNSLY 301

Query: 300 KSKDEESKFNKTSASEQVMEELHRLIDFFTQKGDREISLTIVGHSLGGALSLLTAYEAGA 359
            + D+ +     S +E ++ E+ RL++ +      E+S+++ GHSLG A++LL A +   
Sbjct: 302 TTGDQHA----PSLAESLVGEISRLVELY---AGEELSISVTGHSLGAAIALLAADDIAE 361

Query: 360 SFPDI-HVSVMSFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVNKLS 419
             P    V+V SFG PRVGN  F ++L+  GVK LRVV  QD+V K+PG+F ++     S
Sbjct: 362 RVPHAPPVAVFSFGGPRVGNREFADRLDSKGVKVLRVVNSQDVVTKVPGIFADNDKQGQS 421

Query: 420 AVTGKL------------NWLYRHVGTQLKMDMYMSPYLKRESDMSGSHNLEIYLHLVDG 475
              G+              W Y HVG +L++DM MSPYLK  +D++  H+LE YLHLVDG
Sbjct: 422 RNNGRSPGGIMEMVERNNPWAYSHVGAELRVDMKMSPYLKPNADVACCHDLEAYLHLVDG 478

BLAST of Cp4.1LG20g05490 vs. NCBI nr
Match: gi|659068501|ref|XP_008444745.1| (PREDICTED: phospholipase A1-Igamma1, chloroplastic [Cucumis melo])

HSP 1 Score: 966.1 bits (2496), Expect = 2.6e-278
Identity = 467/536 (87.13%), Postives = 502/536 (93.66%), Query Frame = 1

Query: 1   MEGRIVTPRGVPLKRVTMLRNGTKKTKWYWKLKWGIRLRAIKNALSSSLHNQRRRLTCAT 60
           ME ++ TPRG+P+KR TMLRNGTKKTKWYWKLK GIR RAIK ALSSSLHNQRRRLTC+T
Sbjct: 1   MEAKLATPRGIPVKRTTMLRNGTKKTKWYWKLKLGIRFRAIKAALSSSLHNQRRRLTCST 60

Query: 61  A-----TGQLTPVNGAASPLVIQPSKSTSKKNDLRLAKPLASLLRMPQRAADFVDYGNHM 120
           A     T QLTP NG  SPLVI  SK  SKKNDLRLAKPLASLLRMP RAADF+D+GNHM
Sbjct: 61  AITTTTTPQLTPTNGLVSPLVIHQSKP-SKKNDLRLAKPLASLLRMPLRAADFIDFGNHM 120

Query: 121 TPTFSPRDKIADVWRDLHGGSNWEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEF 180
           TPT SPRD I+ VWRDLHG S+WEGLLDPLHPFLRRE+VKYGEFAQATYDAFDFDPLSEF
Sbjct: 121 TPTLSPRDNISAVWRDLHGASDWEGLLDPLHPFLRREIVKYGEFAQATYDAFDFDPLSEF 180

Query: 181 RGSCRYNRHKLLHELGLAQNGYKVTRYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVA 240
            GSCRYNRHKLL+ELGLAQNGYKVT+YIYALSPVDGPDWFESSKIGEVWSRDSNWMG+VA
Sbjct: 181 CGSCRYNRHKLLNELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGYVA 240

Query: 241 VSNDEESERIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRSDRKIKVQRGFLTIYKSKD 300
           VS+DEES+RIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRS++K+KVQRGFLTIYKSKD
Sbjct: 241 VSSDEESDRIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRSNKKVKVQRGFLTIYKSKD 300

Query: 301 EESKFNKTSASEQVMEELHRLIDFFTQKGDREISLTIVGHSLGGALSLLTAYEAGASFP- 360
           E+SKFNKTSASEQVMEELHRLIDFF +KGDREISLTI GHSLGGALSLLTAYEAG +FP 
Sbjct: 301 EDSKFNKTSASEQVMEELHRLIDFFKEKGDREISLTITGHSLGGALSLLTAYEAGVTFPA 360

Query: 361 DIHVSVMSFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVNKLSAVTG 420
           D+HVSV+SFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVNKL AVTG
Sbjct: 361 DVHVSVVSFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVNKLGAVTG 420

Query: 421 KLNWLYRHVGTQLKMDMYMSPYLKRESDMSGSHNLEIYLHLVDGFVSRRGKFRWNSRRDV 480
           KLNW+YRHVG +L+M+MYMSPYLK++SDMSGSHNLEIYLHLVDGFV+++GKFRWNSRRDV
Sbjct: 421 KLNWVYRHVGKELRMNMYMSPYLKKDSDMSGSHNLEIYLHLVDGFVAKKGKFRWNSRRDV 480

Query: 481 ALVNKGSDMLVEELRIPEFWYQLPHKGLVKNRFGRWVKPGRNAEDIPSPFSRPSNI 531
           ALVNKGSDMLVEELRIPEFWYQLPHKGL+KNRFGRWVKPGRN EDIPSPFS+P  +
Sbjct: 481 ALVNKGSDMLVEELRIPEFWYQLPHKGLIKNRFGRWVKPGRNPEDIPSPFSQPLQV 535

BLAST of Cp4.1LG20g05490 vs. NCBI nr
Match: gi|449435504|ref|XP_004135535.1| (PREDICTED: phospholipase A1-Igamma1, chloroplastic [Cucumis sativus])

HSP 1 Score: 958.0 bits (2475), Expect = 7.0e-276
Identity = 464/535 (86.73%), Postives = 501/535 (93.64%), Query Frame = 1

Query: 1   MEGRIVTPRGVPLKRVTMLRNGTKKTKWYWKLKWGIRLRAIKNALSSSLHNQRRRLTCAT 60
           ME +  TPRG+P+KR TMLRNGTKKTKWYWKLK GI+ +AIK ALSSSLHNQRRRLTC+T
Sbjct: 1   MEPKFATPRGIPVKRTTMLRNGTKKTKWYWKLKLGIQFKAIKAALSSSLHNQRRRLTCST 60

Query: 61  A----TGQLTPVNGAASPLVIQPSKSTSKKNDLRLAKPLASLLRMPQRAADFVDYGNHMT 120
           A    T Q TP NG  SPLVI  SKS SKKNDLRLAKPLASLLRMP RA+DF+D+GNHMT
Sbjct: 61  AITTTTPQHTPTNGLVSPLVIHQSKS-SKKNDLRLAKPLASLLRMPLRASDFIDFGNHMT 120

Query: 121 PTFSPRDKIADVWRDLHGGSNWEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFR 180
           PT SPRDKI+ VWRDLHG S+WEGLLDPLHPFLRRE+VKYGEFAQATYDAFDFDPLSEF 
Sbjct: 121 PTLSPRDKISAVWRDLHGASDWEGLLDPLHPFLRREIVKYGEFAQATYDAFDFDPLSEFC 180

Query: 181 GSCRYNRHKLLHELGLAQNGYKVTRYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAV 240
           GSCRYNR KLL+ELGLAQNGYKVT+YIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAV
Sbjct: 181 GSCRYNRQKLLNELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAV 240

Query: 241 SNDEESERIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRSDRKIKVQRGFLTIYKSKDE 300
           S+DEES+RIGRRDILV+WRGTVTPTEWYIDLKTKLKKIDRS++K+KVQRGFLTIYKSKDE
Sbjct: 241 SSDEESDRIGRRDILVSWRGTVTPTEWYIDLKTKLKKIDRSNKKVKVQRGFLTIYKSKDE 300

Query: 301 ESKFNKTSASEQVMEELHRLIDFFTQKGDREISLTIVGHSLGGALSLLTAYEAGASFP-D 360
           +SKFNKTSASEQVMEELHRLIDFF QKGDREISLTI GHSLGGALSLLTAYEAG +FP D
Sbjct: 301 DSKFNKTSASEQVMEELHRLIDFFKQKGDREISLTITGHSLGGALSLLTAYEAGVTFPAD 360

Query: 361 IHVSVMSFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVNKLSAVTGK 420
           +HVSV+SFGAPRVGNLAFREKLNEMGVK LRVVI QDIVPKLPGLFVNSIVNKLSAVTGK
Sbjct: 361 VHVSVVSFGAPRVGNLAFREKLNEMGVKILRVVIGQDIVPKLPGLFVNSIVNKLSAVTGK 420

Query: 421 LNWLYRHVGTQLKMDMYMSPYLKRESDMSGSHNLEIYLHLVDGFVSRRGKFRWNSRRDVA 480
           LNW+YRHVG +L+M+M+MSPYLK++SDMSGSHNLEIYLHLVDGFV+++GKFRWNSRRDVA
Sbjct: 421 LNWVYRHVGKELRMNMFMSPYLKKDSDMSGSHNLEIYLHLVDGFVTKKGKFRWNSRRDVA 480

Query: 481 LVNKGSDMLVEELRIPEFWYQLPHKGLVKNRFGRWVKPGRNAEDIPSPFSRPSNI 531
           LVNKGSDMLVEELRIPEFWYQLPHKGL+KNRFGRWVKPGRN EDIPSPFS+PS +
Sbjct: 481 LVNKGSDMLVEELRIPEFWYQLPHKGLIKNRFGRWVKPGRNVEDIPSPFSQPSQV 534

BLAST of Cp4.1LG20g05490 vs. NCBI nr
Match: gi|566186599|ref|XP_002314049.2| (hypothetical protein POPTR_0009s06300g [Populus trichocarpa])

HSP 1 Score: 662.9 bits (1709), Expect = 4.6e-187
Identity = 320/518 (61.78%), Postives = 408/518 (78.76%), Query Frame = 1

Query: 12  PLKRVTMLRNGTKKTKWYWKLKWGIRLRAIKNALSSSLHNQRR---RLTCATATGQLTPV 71
           P ++VT     ++KT+  WKLK  I   AIK A+S ++  +      L+CA +  +L+  
Sbjct: 5   PFRQVTTEETRSRKTRRAWKLKLNITWHAIKKAVSYNVKKRLHLHLHLSCARSMRRLS-T 64

Query: 72  NGAASPLVIQPSKSTSKKNDLRL-AKPLASLLRMPQRAADFVDYGNHMTPTFSPRDKIAD 131
           N     L ++P +++  K   R  +K LASLL +P  A DFVD G+HMTPT SP+  I+ 
Sbjct: 65  NQTHQELAVKPHQASKPKQKARNPSKTLASLLHVPYTALDFVDRGDHMTPTLSPKQSISA 124

Query: 132 VWRDLHGGSNWEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFRGSCRYNRHKLL 191
           VW+++HG SNWE LLDP HP LRRE++KYGEFAQ TYDAFDFDPLS+F GSCRYNR K  
Sbjct: 125 VWKEIHGSSNWETLLDPFHPSLRREILKYGEFAQGTYDAFDFDPLSDFCGSCRYNRRKFF 184

Query: 192 HELGLAQNGYKVTRYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSNDEESERIGR 251
             LGL ++GYKV +YIYALS VD P+W + S     WS+DSNWMG+VAVS  EES+RIGR
Sbjct: 185 ETLGLTKHGYKVKKYIYALSHVDVPEWLKRSYA--TWSKDSNWMGYVAVSRREESQRIGR 244

Query: 252 RDILVAWRGTVTPTEWYIDLKTKLKKIDRSDRKIKVQRGFLTIYKSKDEESKFNKTSASE 311
           RDI+VAWRGTV+P+EW+ DL T L+ ID ++  +KVQ GFL++YKSKDE +++NK SASE
Sbjct: 245 RDIMVAWRGTVSPSEWFKDLTTSLEHIDNTN--VKVQEGFLSVYKSKDELTRYNKLSASE 304

Query: 312 QVMEELHRLIDFFTQKGDREISLTIVGHSLGGALSLLTAYEAGASFPDIHVSVMSFGAPR 371
           QVM+E+ RL++F+  KG+ E+SLT+ GHSLGGAL+LL AYEA  + PD+ VSV+SFGAPR
Sbjct: 305 QVMQEVMRLVNFYRGKGE-EVSLTVTGHSLGGALALLNAYEAATAIPDLFVSVISFGAPR 364

Query: 372 VGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVNKLSAVTGKLNWLYRHVGTQL 431
           VGN+AF+EKLNE+GVKTLRVV++QDIVPKLPGL +N ++NK   +TGKLNW+YRHVGTQL
Sbjct: 365 VGNIAFKEKLNELGVKTLRVVVKQDIVPKLPGL-LNKMLNKFHGLTGKLNWVYRHVGTQL 424

Query: 432 KMDMYMSPYLKRESDMSGSHNLEIYLHLVDGFVSRRGKFRWNSRRDVALVNKGSDMLVEE 491
           K+D +MSPYLK ESD+SGSHNLE+YLHL+DGF S++ K+RWN+RRD+ALVNKGSDML+E+
Sbjct: 425 KLDAFMSPYLKPESDLSGSHNLELYLHLIDGFFSKKSKYRWNARRDLALVNKGSDMLIED 484

Query: 492 LRIPEFWYQLPHKGLVKNRFGRWVKPGRNAEDIPSPFS 526
           L+IPEFWYQ P+KGLV N++GRWVKPGR  EDIPSP S
Sbjct: 485 LKIPEFWYQFPYKGLVLNQYGRWVKPGRLPEDIPSPLS 515

BLAST of Cp4.1LG20g05490 vs. NCBI nr
Match: gi|802596321|ref|XP_012072182.1| (PREDICTED: phospholipase A1-Igamma1, chloroplastic [Jatropha curcas])

HSP 1 Score: 661.8 bits (1706), Expect = 1.0e-186
Identity = 317/515 (61.55%), Postives = 409/515 (79.42%), Query Frame = 1

Query: 12  PLKR-VTMLRNGTKKTKWYWKLKWGIRLRAIKNALSSSLHNQRRRLTCATATGQLTPVNG 71
           PLKR V   +  ++KT+  WKLK  I  +AIK A +S   N+  RL+C     QL+ +N 
Sbjct: 5   PLKRRVLAEKTRSRKTRRVWKLKLNITWQAIKKAFTSK--NRLLRLSCV----QLSTIN- 64

Query: 72  AASPLVIQPSKSTSKKNDLRLAKPLASLLRMPQRAADFVDYGNHMTPTFSPRDKIADVWR 131
                + Q +K   +KN  +  K LA+LL +P  A+DF+D G+ MTPT SP++ ++  WR
Sbjct: 65  -----IHQDAKK--RKNSSKPHKTLANLLHVPYTASDFIDRGDQMTPTLSPKENVSAKWR 124

Query: 132 DLHGGSNWEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFRGSCRYNRHKLLHEL 191
           ++HG  NWE LLDPL P LRRE++KYGEFA ATYDAFDFDPLSE+ GSCRYNRHK+  EL
Sbjct: 125 EIHGSCNWENLLDPLDPCLRREILKYGEFATATYDAFDFDPLSEYCGSCRYNRHKIFEEL 184

Query: 192 GLAQNGYKVTRYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSNDEESERIGRRDI 251
           GL ++ Y VT+YIYA+S VD P+WF  +    +WS+DSNWMG+VAVSND+ES+R+GRRDI
Sbjct: 185 GLNKHNYNVTKYIYAMSHVDVPEWFVRAH--SIWSKDSNWMGYVAVSNDQESKRLGRRDI 244

Query: 252 LVAWRGTVTPTEWYIDLKTKLKKIDRSDRKIKVQRGFLTIYKSKDEESKFNKTSASEQVM 311
           +VAWRGTV PTEW+ DLKTKL++ID  + KIKVQ GF +IYKSK E +++NK SASEQVM
Sbjct: 245 VVAWRGTVAPTEWFTDLKTKLEEIDEENEKIKVQYGFYSIYKSKSEFTRYNKLSASEQVM 304

Query: 312 EELHRLIDFFTQKGDREISLTIVGHSLGGALSLLTAYEAGASFPDIHVSVMSFGAPRVGN 371
           +E+ +L++ + + G+ E+SLT+ GHSLGGAL++L AYEA  S P++ VSV+SFGAPRVGN
Sbjct: 305 QEIRKLVNCYRENGE-EVSLTVTGHSLGGALAVLNAYEAANSIPNLFVSVISFGAPRVGN 364

Query: 372 LAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVNKLSAVTGKLNWLYRHVGTQLKMD 431
           LAF+EKLNE+GVKTLRVV++QDIVPK+PG+ VN I+ K++ +T +LNW+YRHVGTQLK+D
Sbjct: 365 LAFKEKLNELGVKTLRVVVKQDIVPKVPGIIVNKILRKINTITQRLNWVYRHVGTQLKLD 424

Query: 432 MYMSPYLKRESDMSGSHNLEIYLHLVDGFVSRRGKFRWNSRRDVALVNKGSDMLVEELRI 491
           M+MSPYLK  SD SGSHNLE+YLHL+DGF+ ++ +FRW++RRD+ALVNK SDML+EEL+I
Sbjct: 425 MFMSPYLKHVSDFSGSHNLEVYLHLLDGFLGKKLQFRWHARRDLALVNKSSDMLIEELKI 484

Query: 492 PEFWYQLPHKGLVKNRFGRWVKPGRNAEDIPSPFS 526
           PEFWYQLPHKGLV N++GRWVKP R  EDIPSPFS
Sbjct: 485 PEFWYQLPHKGLVLNKYGRWVKPTREPEDIPSPFS 502

BLAST of Cp4.1LG20g05490 vs. NCBI nr
Match: gi|743837416|ref|XP_011025479.1| (PREDICTED: phospholipase A1-Igamma1, chloroplastic-like [Populus euphratica])

HSP 1 Score: 661.0 bits (1704), Expect = 1.8e-186
Identity = 319/518 (61.58%), Postives = 407/518 (78.57%), Query Frame = 1

Query: 12  PLKRVTMLRNGTKKTKWYWKLKWGIRLRAIKNALSSSLHNQRR---RLTCATATGQLTPV 71
           P ++VT     ++KT+  WKLK  I   AIK A+S ++  +     +L+CA +  +L+  
Sbjct: 5   PFRQVTTEETRSRKTRRAWKLKLNITWHAIKKAVSYNVKKRLHLHLQLSCARSMRRLS-T 64

Query: 72  NGAASPLVIQPSKSTSKKNDLRL-AKPLASLLRMPQRAADFVDYGNHMTPTFSPRDKIAD 131
           N     L ++P +++  K   R  +K LASLL +P  A DFVD G+HMTPT SP+  I+ 
Sbjct: 65  NQTHQELAVKPHQASKPKQKARNPSKTLASLLHVPYTALDFVDRGDHMTPTLSPKQSISA 124

Query: 132 VWRDLHGGSNWEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFRGSCRYNRHKLL 191
           VW+++HG SNWE LLDPLHP LRRE++KYGEF Q TYDAFDFDPLS+F GSCRYNR K  
Sbjct: 125 VWKEIHGSSNWETLLDPLHPSLRREILKYGEFVQGTYDAFDFDPLSDFCGSCRYNRRKFF 184

Query: 192 HELGLAQNGYKVTRYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSNDEESERIGR 251
             LGL ++GYKV RYIYALS VD P+W + S     WS+DSNWMG+VAVS  EES+RIGR
Sbjct: 185 ETLGLTKHGYKVKRYIYALSHVDVPEWLKRSYT--TWSKDSNWMGYVAVSRKEESQRIGR 244

Query: 252 RDILVAWRGTVTPTEWYIDLKTKLKKIDRSDRKIKVQRGFLTIYKSKDEESKFNKTSASE 311
           RDI+VAWRGTV+P+EW+ DL T L+ ID ++  +KVQ GFL++YKSKDE +++NK  +SE
Sbjct: 245 RDIMVAWRGTVSPSEWFKDLTTSLEHIDNTN--VKVQEGFLSVYKSKDELTRYNKLRSSE 304

Query: 312 QVMEELHRLIDFFTQKGDREISLTIVGHSLGGALSLLTAYEAGASFPDIHVSVMSFGAPR 371
           QVM+E+ RL++F+  KG+ E+SLT+ GHSLGGAL+LL AYEA  + PD+ VSV+SFGAPR
Sbjct: 305 QVMQEVMRLVNFYRGKGE-EVSLTVTGHSLGGALALLNAYEAATAIPDLFVSVISFGAPR 364

Query: 372 VGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVNKLSAVTGKLNWLYRHVGTQL 431
           VGN+AF+EKLNE+GVKTLRVV++QDIVPKLPGL +N ++NK   +TGKLNW+YRHVGTQL
Sbjct: 365 VGNIAFKEKLNELGVKTLRVVVKQDIVPKLPGL-LNKMLNKFHGLTGKLNWVYRHVGTQL 424

Query: 432 KMDMYMSPYLKRESDMSGSHNLEIYLHLVDGFVSRRGKFRWNSRRDVALVNKGSDMLVEE 491
           K+D +MSPYLK ESD+SGSHNLE+YLHL+DGF S++ K+RWN+RRD+ALVNKGSDML+E+
Sbjct: 425 KLDAFMSPYLKHESDLSGSHNLELYLHLIDGFFSKKSKYRWNARRDLALVNKGSDMLIED 484

Query: 492 LRIPEFWYQLPHKGLVKNRFGRWVKPGRNAEDIPSPFS 526
           L+IPEFWYQ P+KGLV N+ GRWVKPGR  EDIPSP S
Sbjct: 485 LKIPEFWYQFPYKGLVLNQHGRWVKPGRLPEDIPSPLS 515

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PLA16_ARATH7.3e-10847.47Phospholipase A1-Igamma2, chloroplastic OS=Arabidopsis thaliana GN=At2g30550 PE=... [more]
PLA15_ARATH2.8e-10742.41Phospholipase A1-Igamma1, chloroplastic OS=Arabidopsis thaliana GN=At1g06800 PE=... [more]
PLA17_ARATH1.8e-9845.23Phospholipase A1-Igamma3, chloroplastic OS=Arabidopsis thaliana GN=At1g51440 PE=... [more]
PLA4_ORYSJ2.7e-7039.85Phospholipase A1-II 4 OS=Oryza sativa subsp. japonica GN=Os01g0652300 PE=2 SV=2[more]
PLA14_ARATH1.1e-6840.43Phospholipase A1-Ibeta2, chloroplastic OS=Arabidopsis thaliana GN=At4g16820 PE=1... [more]
Match NameE-valueIdentityDescription
A0A0A0M111_CUCSA4.8e-27686.73Uncharacterized protein OS=Cucumis sativus GN=Csa_1G537320 PE=4 SV=1[more]
B9HNS4_POPTR3.2e-18761.78Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0009s06300g PE=4 SV=2[more]
A0A067L0J4_JATCU7.2e-18761.55Uncharacterized protein OS=Jatropha curcas GN=JCGZ_04663 PE=4 SV=1[more]
B9SYI5_RICCO1.6e-18661.66Triacylglycerol lipase, putative OS=Ricinus communis GN=RCOM_1288510 PE=4 SV=1[more]
A0A0D2UMJ2_GOSRA5.9e-18160.27Uncharacterized protein OS=Gossypium raimondii GN=B456_009G152400 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G30370.13.3e-16756.73 alpha/beta-Hydrolases superfamily protein[more]
AT2G30550.24.1e-10947.47 alpha/beta-Hydrolases superfamily protein[more]
AT1G06800.11.6e-10842.41 alpha/beta-Hydrolases superfamily protein[more]
AT1G51440.11.0e-9945.23 alpha/beta-Hydrolases superfamily protein[more]
AT4G16820.16.4e-7040.43 alpha/beta-Hydrolases superfamily protein[more]
Match NameE-valueIdentityDescription
gi|659068501|ref|XP_008444745.1|2.6e-27887.13PREDICTED: phospholipase A1-Igamma1, chloroplastic [Cucumis melo][more]
gi|449435504|ref|XP_004135535.1|7.0e-27686.73PREDICTED: phospholipase A1-Igamma1, chloroplastic [Cucumis sativus][more]
gi|566186599|ref|XP_002314049.2|4.6e-18761.78hypothetical protein POPTR_0009s06300g [Populus trichocarpa][more]
gi|802596321|ref|XP_012072182.1|1.0e-18661.55PREDICTED: phospholipase A1-Igamma1, chloroplastic [Jatropha curcas][more]
gi|743837416|ref|XP_011025479.1|1.8e-18661.58PREDICTED: phospholipase A1-Igamma1, chloroplastic-like [Populus euphratica][more]
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0006629lipid metabolic process
Vocabulary: INTERPRO
TermDefinition
IPR002921Fungal_lipase-like
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006629 lipid metabolic process
biological_process GO:0046486 glycerolipid metabolic process
biological_process GO:0009395 phospholipid catabolic process
biological_process GO:0010214 seed coat development
cellular_component GO:0005575 cellular_component
cellular_component GO:0005739 mitochondrion
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0008970 phosphatidylcholine 1-acylhydrolase activity
molecular_function GO:0003674 molecular_function

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG20g05490.1Cp4.1LG20g05490.1mRNA


Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002921Fungal lipase-like domainPFAMPF01764Lipase_3coord: 252..400
score: 9.3
NoneNo IPR availablePANTHERPTHR31403FAMILY NOT NAMEDcoord: 36..530
score: 2.7E
NoneNo IPR availablePANTHERPTHR31403:SF13DAD1-LIKE ACYLHYDROLASEcoord: 36..530
score: 2.7E

The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
Cp4.1LG20g05490Cp4.1LG09g07410Cucurbita pepo (Zucchini)cpecpeB048