Bhi08G001649 (gene) Wax gourd

NameBhi08G001649
Typegene
OrganismBenincasa hispida (Wax gourd)
Descriptionphospholipase A1-Igamma1, chloroplastic
Locationchr8 : 58132575 .. 58134802 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCCTTCTATAAATTCGCAACTAATCCAATCCATTTCATCCCCAAAACAAAACATTTCCGTTTTTTCAAAAGAAAAATTCTGTCTTTTGTCTCTGTCTGTCTCTCTCTCTGTCGCCGGCAACCGAAATCTGTTGGGTTTTAGTTCAGCCATGGAGGCGAAAATGGCTACCCCAAGAGGAGTAGCAATGAAAAGAGTAACAATGTTGAGAAACGGAACAAAGAAAACAAAATGGTATTGGAAATTGAAATTCGGAATCCGATTAAAAGCCATTAGATCCGCCATTTCTTCTTCTCTACACAACCAACGCCGCCGTCTCACATGTTCCACCGCCATCCCCACCAACACTCCGGTAAACGGCATCGTTTCACCATTAGTAGTACACCAATCCAGATCCTCCTCCAAGAAAAACGAACTCCGTTTAGCCAAACCCCTCGCTTCCCTCCTCCGTATGCCACTCCGCGCCGCCGATTTCATCGATTTCGGCAACCACATGACTCCCACACTCTCCCCTCGTGAAAACATCTCCGCCGTCTGGCGTGACCTCCACGGCGCCTCCGACTGGGACGGCCTCCTCGACCCTCTCCACCCTTTCCTCCGCCGTGAAATCGTCAAATACGGCGAATTCGCTCAAGCAACTTACGACGCTTTTGACTTCGACCCACTTTCTGAATTCTGCGGTAGTTGCAGATACAACCGCCACAAACTCTTCAACGAATTGGGTTTAGCTCAAAACGGCTACAAAGTTACAAAATACATCTACGCGTTGTCCCCTGTTGACGGCCCTGATTGGTTCGAAAGCTCCAAAATCGGCGAAGTCTGGAGCCGGGATTCGAATTGGATGGGATTTGTCGCCGTTAGCACCGATGAAGAAAGTGACAGAATCGGGCGGAGAGATATCCTCGTTGCTTGGAGAGGGACGGTTACACCGACGGAATGGTATATCGATTTAAAAACCAAGTTGAAGAAAATCGACCGGAGCGACAAGAAAGTGAAAGTCCAACGTGGGTTTTTAACAATTTACAAATCCAAAGACGAGGAATCAAAATTCAACAAAACCAGCGCATCGGAGCAAGTAATGGAAGAGTTAAACAGACTAATAAATTTCTTCAAAGAAAAAGGCGACAGAGAAATCAGTTTAACCATAACAGGGCACAGTCTCGGCGGTGCTCTGTCTCTGTTAACAGCGTACGAAGCAGGGGTTTTATTCCCGACGGGCGTACACATCAGCGTGGTGTCATTCGGAGCTCCGAGAGTTGGGAATTTAACGTTTAGAGAGAAATTAAACGAAATGGGAGTGAAAACGCTACGTGTAGTGATCGGACAAGACATTGTTCCAAAACTTCCGGGTTTGTTTGTGAATTCGATAGTGAACAAGTTGAGCTTGGTGACGGGGAAGTTGAATTGGGTGTATCGACATGTGGGGAAGGAATTGAGGATGGATATGTATATGTCGCCGTATTTGAAGAGGGAATCCGACATGACGGGGAGTCATAATTTGGAGATATATTTGCATTTGGTAGATGGGTTTGTTAGTAGGAAAGGGAAATTTAGGTGGAATTCGAGAAGGGATGTGGCGTTGGTGAACAAAGGGAGTGATATGCTTGTGGAAGAATTGAGAATTCCTCAGTTTTGGTATCAATTGCCACATAAAGGATTGATTAAGAATAGGTTTGGGAGATGGGTTAAGCCTGGGAGAAACCCTGAAGATATTCCTTCACCATTTTCTCAACCTTCCCATGTTTGAGATTATTCCTCCTTCTTTATTTATTTATTGTTTACCCCTTTTTTTTAAAAATGATTTTGTGAATTGGATCAATTGATGTAGATGATTTGATTAATTCTTTTTTTAAGAGGAAGTTTGGAATGATATGGATGAGTCATTTTCGCAAATTTTAGTTCGGAGGAAGATTCTTGAATCTCAATTAACTATATAACCTAATTGGTTTACATATATAAACTCTCGATTAAAATATTCAAGGATTCGAACCTCTCAACTTAACTCTAAGTTATAGAAACGGTATACAAGATGTAGGCCTCCTAAAAAAGGATGTATGTAAGGTTATTAGTTGGAGAGATGATCTTAAGCTAATGAATATATATAGATCTAAAGGCATAAATCAAAGTTCTTCTAATTTTTTTTTTTACATATATAGAAATGTTTATTTTCATTATACAGACTTTTCGTCTAATATTGAATTAATATAGTTTGAGAAAGACGAATGGATGAG

mRNA sequence

CCCTTCTATAAATTCGCAACTAATCCAATCCATTTCATCCCCAAAACAAAACATTTCCGTTTTTTCAAAAGAAAAATTCTGTCTTTTGTCTCTGTCTGTCTCTCTCTCTGTCGCCGGCAACCGAAATCTGTTGGGTTTTAGTTCAGCCATGGAGGCGAAAATGGCTACCCCAAGAGGAGTAGCAATGAAAAGAGTAACAATGTTGAGAAACGGAACAAAGAAAACAAAATGGTATTGGAAATTGAAATTCGGAATCCGATTAAAAGCCATTAGATCCGCCATTTCTTCTTCTCTACACAACCAACGCCGCCGTCTCACATGTTCCACCGCCATCCCCACCAACACTCCGGTAAACGGCATCGTTTCACCATTAGTAGTACACCAATCCAGATCCTCCTCCAAGAAAAACGAACTCCGTTTAGCCAAACCCCTCGCTTCCCTCCTCCGTATGCCACTCCGCGCCGCCGATTTCATCGATTTCGGCAACCACATGACTCCCACACTCTCCCCTCGTGAAAACATCTCCGCCGTCTGGCGTGACCTCCACGGCGCCTCCGACTGGGACGGCCTCCTCGACCCTCTCCACCCTTTCCTCCGCCGTGAAATCGTCAAATACGGCGAATTCGCTCAAGCAACTTACGACGCTTTTGACTTCGACCCACTTTCTGAATTCTGCGGTAGTTGCAGATACAACCGCCACAAACTCTTCAACGAATTGGGTTTAGCTCAAAACGGCTACAAAGTTACAAAATACATCTACGCGTTGTCCCCTGTTGACGGCCCTGATTGGTTCGAAAGCTCCAAAATCGGCGAAGTCTGGAGCCGGGATTCGAATTGGATGGGATTTGTCGCCGTTAGCACCGATGAAGAAAGTGACAGAATCGGGCGGAGAGATATCCTCGTTGCTTGGAGAGGGACGGTTACACCGACGGAATGGTATATCGATTTAAAAACCAAGTTGAAGAAAATCGACCGGAGCGACAAGAAAGTGAAAGTCCAACGTGGGTTTTTAACAATTTACAAATCCAAAGACGAGGAATCAAAATTCAACAAAACCAGCGCATCGGAGCAAGTAATGGAAGAGTTAAACAGACTAATAAATTTCTTCAAAGAAAAAGGCGACAGAGAAATCAGTTTAACCATAACAGGGCACAGTCTCGGCGGTGCTCTGTCTCTGTTAACAGCGTACGAAGCAGGGGTTTTATTCCCGACGGGCGTACACATCAGCGTGGTGTCATTCGGAGCTCCGAGAGTTGGGAATTTAACGTTTAGAGAGAAATTAAACGAAATGGGAGTGAAAACGCTACGTGTAGTGATCGGACAAGACATTGTTCCAAAACTTCCGGGTTTGTTTGTGAATTCGATAGTGAACAAGTTGAGCTTGGTGACGGGGAAGTTGAATTGGGTGTATCGACATGTGGGGAAGGAATTGAGGATGGATATGTATATGTCGCCGTATTTGAAGAGGGAATCCGACATGACGGGGAGTCATAATTTGGAGATATATTTGCATTTGGTAGATGGGTTTGTTAGTAGGAAAGGGAAATTTAGGTGGAATTCGAGAAGGGATGTGGCGTTGGTGAACAAAGGGAGTGATATGCTTGTGGAAGAATTGAGAATTCCTCAGTTTTGGTATCAATTGCCACATAAAGGATTGATTAAGAATAGGTTTGGGAGATGGGTTAAGCCTGGGAGAAACCCTGAAGATATTCCTTCACCATTTTCTCAACCTTCCCATGTTTGAGATTATTCCTCCTTCTTTATTTATTTATTGTTTACCCCTTTTTTTTAAAAATGATTTTGTGAATTGGATCAATTGATGTAGATGATTTGATTAATTCTTTTTTTAAGAGGAAGTTTGGAATGATATGGATGAGTCATTTTCGCAAATTTTAGTTCGGAGGAAGATTCTTGAATCTCAATTAACTATATAACCTAATTGGTTTACATATATAAACTCTCGATTAAAATATTCAAGGATTCGAACCTCTCAACTTAACTCTAAGTTATAGAAACGGTATACAAGATGTAGGCCTCCTAAAAAAGGATGTATGTAAGGTTATTAGTTGGAGAGATGATCTTAAGCTAATGAATATATATAGATCTAAAGGCATAAATCAAAGTTCTTCTAATTTTTTTTTTTACATATATAGAAATGTTTATTTTCATTATACAGACTTTTCGTCTAATATTGAATTAATATAGTTTGAGAAAGACGAATGGATGAG

Coding sequence (CDS)

CCTTCTATAAATTCGCAACTAATCCAATCCATTTCATCCCCAAAACAAAACATTTCCGTTTTTTCAAAAGAAAAATTCTGTCTTTTGTCTCTGTCTGTCTCTCTCTCTGTCGCCGGCAACCGAAATCTGTTGGGTTTTAGTTCAGCCATGGAGGCGAAAATGGCTACCCCAAGAGGAGTAGCAATGAAAAGAGTAACAATGTTGAGAAACGGAACAAAGAAAACAAAATGGTATTGGAAATTGAAATTCGGAATCCGATTAAAAGCCATTAGATCCGCCATTTCTTCTTCTCTACACAACCAACGCCGCCGTCTCACATGTTCCACCGCCATCCCCACCAACACTCCGGTAAACGGCATCGTTTCACCATTAGTAGTACACCAATCCAGATCCTCCTCCAAGAAAAACGAACTCCGTTTAGCCAAACCCCTCGCTTCCCTCCTCCGTATGCCACTCCGCGCCGCCGATTTCATCGATTTCGGCAACCACATGACTCCCACACTCTCCCCTCGTGAAAACATCTCCGCCGTCTGGCGTGACCTCCACGGCGCCTCCGACTGGGACGGCCTCCTCGACCCTCTCCACCCTTTCCTCCGCCGTGAAATCGTCAAATACGGCGAATTCGCTCAAGCAACTTACGACGCTTTTGACTTCGACCCACTTTCTGAATTCTGCGGTAGTTGCAGATACAACCGCCACAAACTCTTCAACGAATTGGGTTTAGCTCAAAACGGCTACAAAGTTACAAAATACATCTACGCGTTGTCCCCTGTTGACGGCCCTGATTGGTTCGAAAGCTCCAAAATCGGCGAAGTCTGGAGCCGGGATTCGAATTGGATGGGATTTGTCGCCGTTAGCACCGATGAAGAAAGTGACAGAATCGGGCGGAGAGATATCCTCGTTGCTTGGAGAGGGACGGTTACACCGACGGAATGGTATATCGATTTAAAAACCAAGTTGAAGAAAATCGACCGGAGCGACAAGAAAGTGAAAGTCCAACGTGGGTTTTTAACAATTTACAAATCCAAAGACGAGGAATCAAAATTCAACAAAACCAGCGCATCGGAGCAAGTAATGGAAGAGTTAAACAGACTAATAAATTTCTTCAAAGAAAAAGGCGACAGAGAAATCAGTTTAACCATAACAGGGCACAGTCTCGGCGGTGCTCTGTCTCTGTTAACAGCGTACGAAGCAGGGGTTTTATTCCCGACGGGCGTACACATCAGCGTGGTGTCATTCGGAGCTCCGAGAGTTGGGAATTTAACGTTTAGAGAGAAATTAAACGAAATGGGAGTGAAAACGCTACGTGTAGTGATCGGACAAGACATTGTTCCAAAACTTCCGGGTTTGTTTGTGAATTCGATAGTGAACAAGTTGAGCTTGGTGACGGGGAAGTTGAATTGGGTGTATCGACATGTGGGGAAGGAATTGAGGATGGATATGTATATGTCGCCGTATTTGAAGAGGGAATCCGACATGACGGGGAGTCATAATTTGGAGATATATTTGCATTTGGTAGATGGGTTTGTTAGTAGGAAAGGGAAATTTAGGTGGAATTCGAGAAGGGATGTGGCGTTGGTGAACAAAGGGAGTGATATGCTTGTGGAAGAATTGAGAATTCCTCAGTTTTGGTATCAATTGCCACATAAAGGATTGATTAAGAATAGGTTTGGGAGATGGGTTAAGCCTGGGAGAAACCCTGAAGATATTCCTTCACCATTTTCTCAACCTTCCCATGTTTGA

Protein sequence

PSINSQLIQSISSPKQNISVFSKEKFCLLSLSVSLSVAGNRNLLGFSSAMEAKMATPRGVAMKRVTMLRNGTKKTKWYWKLKFGIRLKAIRSAISSSLHNQRRRLTCSTAIPTNTPVNGIVSPLVVHQSRSSSKKNELRLAKPLASLLRMPLRAADFIDFGNHMTPTLSPRENISAVWRDLHGASDWDGLLDPLHPFLRREIVKYGEFAQATYDAFDFDPLSEFCGSCRYNRHKLFNELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSTDEESDRIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRSDKKVKVQRGFLTIYKSKDEESKFNKTSASEQVMEELNRLINFFKEKGDREISLTITGHSLGGALSLLTAYEAGVLFPTGVHISVVSFGAPRVGNLTFREKLNEMGVKTLRVVIGQDIVPKLPGLFVNSIVNKLSLVTGKLNWVYRHVGKELRMDMYMSPYLKRESDMTGSHNLEIYLHLVDGFVSRKGKFRWNSRRDVALVNKGSDMLVEELRIPQFWYQLPHKGLIKNRFGRWVKPGRNPEDIPSPFSQPSHV
BLAST of Bhi08G001649 vs. Swiss-Prot
Match: sp|Q3EBR6|PLA16_ARATH (Phospholipase A1-Igamma2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At2g30550 PE=1 SV=2)

HSP 1 Score: 395.2 bits (1014), Expect = 1.3e-108
Identity = 196/410 (47.80%), Postives = 276/410 (67.32%), Query Frame = 0

Query: 178 WRDLHGASDWDGLLDPLHPFLRREIVKYGEFAQATYDAFDFDPLSEFCGSCRYNRHKLFN 237
           WR + G  DW GL+DP+ P LR E+++YGE AQA YDAFDFDP S++CG+ R+ R + F+
Sbjct: 105 WRKIQGEDDWAGLMDPMDPILRSELIRYGEMAQACYDAFDFDPASKYCGTSRFTRLEFFD 164

Query: 238 ELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSTDEES-DRIGR 297
            LG+  +GY+V +Y+YA S ++ P++F  S+  +VWS+++NWMG+VAVS DE S +R+GR
Sbjct: 165 SLGMIDSGYEVARYLYATSNINLPNFFSKSRWSKVWSKNANWMGYVAVSDDETSRNRLGR 224

Query: 298 RDILVAWRGTVTPTEWYIDLKTKLK-----KIDRSDKKVKVQRGFLTIYKSKDEESKFNK 357
           RDI +AWRGTVT  EW  DLK  LK     KI   D  VKV+ GFL +Y  KD   KF +
Sbjct: 225 RDIAIAWRGTVTKLEWIADLKDYLKPVTENKIRCPDPAVKVESGFLDLYTDKDTTCKFAR 284

Query: 358 TSASEQVMEELNRLINFFKEKGDREISLTITGHSLGGALSLLTAYEAGVLFPTG------ 417
            SA EQ++ E+ RL+    +  D ++S+T+TGHSLGGAL++L+AY+   +          
Sbjct: 285 FSAREQILTEVKRLVEEHGDDDDSDLSITVTGHSLGGALAILSAYDIAEMRLNRSKKGKV 344

Query: 418 VHISVVSFGAPRVGNLTFREKLNEMGVKTLRVVIGQDIVPKLPGLFVN-SIVNKLSLVTG 477
           + ++V+++G PRVGN+ FRE++ E+GVK +RVV   D+VPK PGLF+N S  + L  +  
Sbjct: 345 IPVTVLTYGGPRVGNVRFRERMEELGVKVMRVVNVHDVVPKSPGLFLNESRPHALMKIAE 404

Query: 478 KLNWVYRHVGKELRMDMYMSPYLKRESDMTGSHNLEIYLHLVDGFVSRKGKFRWNSRRDV 537
            L W Y HVG+EL +D   SP+LK   D++ +HNLE  LHL+DG+  +  +F  +S RD 
Sbjct: 405 GLPWCYSHVGEELALDHQNSPFLKPSVDVSTAHNLEAMLHLLDGYHGKGERFVLSSGRDH 464

Query: 538 ALVNKGSDMLVEELRIPQFWYQLPHKGLIKNRFGRWVKPGR-NPEDIPSP 574
           ALVNK SD L E L+IP FW Q  +KG+++N  GRW++  R   ED  SP
Sbjct: 465 ALVNKASDFLKEHLQIPPFWRQDANKGMVRNSEGRWIQAERLRFEDHHSP 514

BLAST of Bhi08G001649 vs. Swiss-Prot
Match: sp|Q941F1|PLA15_ARATH (Phospholipase A1-Igamma1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At1g06800 PE=1 SV=2)

HSP 1 Score: 389.8 bits (1000), Expect = 5.3e-107
Identity = 185/402 (46.02%), Postives = 265/402 (65.92%), Query Frame = 0

Query: 178 WRDLHGASDWDGLLDPLHPFLRREIVKYGEFAQATYDAFDFDPLSEFCGSCRYNRHKLFN 237
           WR + G  DW GL+DP+ P LR E+++YGE AQA YDAFDFDP S +CGSCR+ R  LF+
Sbjct: 89  WRKIQGEDDWAGLMDPMDPVLRSELIRYGEMAQACYDAFDFDPFSRYCGSCRFTRRHLFD 148

Query: 238 ELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSTDEESD--RIG 297
            LG+  +GY+V +Y+YA S ++ P++F  S+  +VWS+++NWMG+VAVS D E+   R+G
Sbjct: 149 SLGIIDSGYEVARYLYATSNINLPNFFSKSRWSKVWSKNANWMGYVAVSDDNEATRCRLG 208

Query: 298 RRDILVAWRGTVTPTEWYIDLKTKLKKIDRS-----DKKVKVQRGFLTIYKSKDEESKFN 357
           RRDI +AWRGTVT  EW  DLK  LK +  +     D  VK + GFL +Y  KD    F+
Sbjct: 209 RRDIAIAWRGTVTRLEWIADLKDFLKPVSGNGFRCPDPAVKAESGFLDLYTDKDTSCNFS 268

Query: 358 KTSASEQVMEELNRLINFFKEKGDREISLTITGHSLGGALSLLTAYEAGVLFPTG----- 417
           K SA EQV+ E+ RL+  + ++   E+S+T+TGHSLGGAL++L+AY+   +         
Sbjct: 269 KFSAREQVLTEVKRLVERYGDEEGEELSITVTGHSLGGALAVLSAYDVAEMGVNRTRKGK 328

Query: 418 -VHISVVSFGAPRVGNLTFREKLNEMGVKTLRVVIGQDIVPKLPGLFVNS-IVNKLSLVT 477
            + ++  ++G PRVGN+ F+E++ ++GVK LRVV   D+V K PGLF+N      L  + 
Sbjct: 329 VIPVTAFTYGGPRVGNIRFKERIEKLGVKVLRVVNEHDVVAKSPGLFLNERAPQALMKLA 388

Query: 478 GKLNWVYRHVGKELRMDMYMSPYLKRESDMTGSHNLEIYLHLVDGFVSRKGKFRWNSRRD 537
           G L W Y HVG+ L +D   SP+LK   D++ +HNLE  LHL+DG+  +  +F  +S RD
Sbjct: 389 GGLPWCYSHVGEMLPLDHQKSPFLKPTVDLSTAHNLEALLHLLDGYHGKGQRFVLSSGRD 448

Query: 538 VALVNKGSDMLVEELRIPQFWYQLPHKGLIKNRFGRWVKPGR 566
            ALVNK SD L +   +P +W Q  +KG+++N  GRW++P R
Sbjct: 449 PALVNKASDFLKDHFMVPPYWRQDANKGMVRNTDGRWIQPDR 490

BLAST of Bhi08G001649 vs. Swiss-Prot
Match: sp|Q9C8J6|PLA17_ARATH (Phospholipase A1-Igamma3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At1g51440 PE=1 SV=1)

HSP 1 Score: 369.0 bits (946), Expect = 9.6e-101
Identity = 192/423 (45.39%), Postives = 269/423 (63.59%), Query Frame = 0

Query: 177 VWRDLHGASDWDGLLDPLHPFLRREIVKYGEFAQATYDAFDFDPLSEFCGSCRYNRHKLF 236
           +WR++ G ++W+G LDP++  LRREI++YGEFAQA YD+FDFDP S++CGSC+Y+    F
Sbjct: 90  IWREVQGCNNWEGQLDPMNNHLRREIIRYGEFAQACYDSFDFDPHSKYCGSCKYHPSDFF 149

Query: 237 NELGL-AQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSTDEES-DRI 296
             L L    GY +T+Y+YA S ++ P++F+ SK+  +WS+ +NWMGFVAV+TDEE   R+
Sbjct: 150 LNLDLHLHKGYTITRYLYATSNINLPNFFQKSKLSSIWSQHANWMGFVAVATDEEEVSRL 209

Query: 297 GRRDILVAWRGTVTPTEWYIDLKTKLKKID-RSDKKVKVQRGFLTIYKSKDEESKFNKTS 356
           GRRDI++AWRGTVT  EW  DLK  L   +   D  +K++ GF  +Y  K++  KF+  S
Sbjct: 210 GRRDIVIAWRGTVTYLEWIYDLKDILCSANFGDDPSIKIELGFHDLYTKKEDSCKFSSFS 269

Query: 357 ASEQVMEELNRLINFF-KEKGDREISLTITGHSLGGALSLLTAYEAGVL----FPTG--- 416
           A EQV+ E+ RLI ++  E+   + S+T+TGHSLG +L+L++AY+   L     P     
Sbjct: 270 AREQVLAEVKRLIEYYGTEEEGHKTSITVTGHSLGASLALVSAYDIAELNLNHVPENNYK 329

Query: 417 VHISVVSFGAPRVGNLTFREKLNEMGVKTLRVVIGQDIVPKLPGLFVNSIVNKLSLVTGK 476
           + I+V SF  PRVGNL F+E+ +E+GVK LRVV   D VP +PG+F N        V  K
Sbjct: 330 IPITVFSFSGPRVGNLRFKERCDELGVKVLRVVNVHDKVPSVPGIFTNEKFQFQKYVEEK 389

Query: 477 LN--WVYRHVGKELRMDMYMSPYLKRESDMTGSHNLEIYLHLVDGFVSR----KGKFRWN 536
            +  W Y HVG EL +D   SP+LK   D+  +HNLE  LHLVDG+  +    + +F   
Sbjct: 390 TSFPWSYAHVGVELALDHKKSPFLKPTKDLGCAHNLEALLHLVDGYHGKDEEAEKRFCLV 449

Query: 537 SRRDVALVNKGSDMLVEELRIPQFWYQLPHKGLIKNRFGRWVKPGR------NPEDIPSP 577
           ++RD+ALVNK  D L  E  +P  W Q  +KG++KN  G+WV P R       PEDI   
Sbjct: 450 TKRDIALVNKSCDFLRGEYHVPPCWRQDENKGMVKNGDGQWVLPDRPLLEPHGPEDIAHH 509

BLAST of Bhi08G001649 vs. Swiss-Prot
Match: sp|O23522|PLA14_ARATH (Phospholipase A1-Ibeta2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At4g16820 PE=1 SV=2)

HSP 1 Score: 263.8 bits (673), Expect = 4.4e-69
Identity = 152/376 (40.43%), Postives = 219/376 (58.24%), Query Frame = 0

Query: 169 SPRENISAVWRDLHGASDWDGLLDPLHPFLRREIVKYGEFAQATYDAFDFDPLSEFCGSC 228
           SP+  + + WR+LHG ++W GLLDPL   LRRE+V+YGEF QA Y AF  DP     GS 
Sbjct: 122 SPKSKLGSKWRELHGLNNWAGLLDPLDENLRRELVRYGEFVQAAYHAFHSDPE----GSP 181

Query: 229 RYNRHKLFNELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVW-SRDSNWMGFVAVST 288
           R+        + L    +KVTK +YA S V  P W +       W ++ ++W+G+VAV  
Sbjct: 182 RH--------VALPDGSFKVTKSLYATSSVRLPKWIDDVAPDLRWMTKQTSWVGYVAVCD 241

Query: 289 D-EESDRIGRRDILVAWRGTVTPTEWYIDLKTKL-----KKIDRSD-KKVKVQRGFLTIY 348
           D  E  R+GRR+I++A RGT T  EW  + +  L      K D+SD  + KV+ GF ++Y
Sbjct: 242 DPREIRRMGRREIVIALRGTATLLEWSENFRPNLVSMPEPKPDQSDPTRPKVECGFNSLY 301

Query: 349 KSKDEESKFNKTSASEQVMEELNRLINFFKEKGDREISLTITGHSLGGALSLLTAYEAGV 408
            + D+ +     S +E ++ E++RL+  +      E+S+++TGHSLG A++LL A +   
Sbjct: 302 TTGDQHA----PSLAESLVGEISRLVELY---AGEELSISVTGHSLGAAIALLAADDIAE 361

Query: 409 LFPTGVHISVVSFGAPRVGNLTFREKLNEMGVKTLRVVIGQDIVPKLPGLFVN------- 468
             P    ++V SFG PRVGN  F ++L+  GVK LRVV  QD+V K+PG+F +       
Sbjct: 362 RVPHAPPVAVFSFGGPRVGNREFADRLDSKGVKVLRVVNSQDVVTKVPGIFADNDKQGQS 421

Query: 469 -----SIVNKLSLVTGKLNWVYRHVGKELRMDMYMSPYLKRESDMTGSHNLEIYLHLVDG 525
                S    + +V     W Y HVG ELR+DM MSPYLK  +D+   H+LE YLHLVDG
Sbjct: 422 RNNGRSPGGIMEMVERNNPWAYSHVGAELRVDMKMSPYLKPNADVACCHDLEAYLHLVDG 478

BLAST of Bhi08G001649 vs. Swiss-Prot
Match: sp|Q9LNC2|PLA18_ARATH (Phospholipase A1-IIalpha OS=Arabidopsis thaliana OX=3702 GN=At1g06250 PE=2 SV=1)

HSP 1 Score: 263.8 bits (673), Expect = 4.4e-69
Identity = 155/409 (37.90%), Postives = 223/409 (54.52%), Query Frame = 0

Query: 172 ENISAVWRDLHGASDWDGLLDPLHPFLRREIVKYGEFAQATYDAFDFDPLSEFCGSCRYN 231
           E I   W+ L G + W GLLDPL P LRR I+ YGE +Q  YDAF++D  S + G C Y+
Sbjct: 3   EGIPKRWKVLSGQNKWKGLLDPLDPDLRRYIIHYGEMSQVGYDAFNWDRKSRYAGDCYYS 62

Query: 232 RHKLFNELGLAQNG---YKVTKYIYALSPVDGPDWFESSKIGEVWSR-DSNWMGFVAVST 291
           +++L    G  +     YKVTKYIYA + +  P  F    + +  SR  +NWMG++AV+T
Sbjct: 63  KNRLLARTGFLKANPFRYKVTKYIYATASIKLPISFIVKSLSKDASRVQTNWMGYIAVAT 122

Query: 292 DEESDRIGRRDILVAWRGTVTPTEWYID----LKTKLKKIDRSDKK--VKVQRGFLTIYK 351
           D+    +GRRDI+VAWRGT+ P EW  D    L+  +     +D K   ++  G+L IY 
Sbjct: 123 DQGKAMLGRRDIVVAWRGTLQPYEWANDFDFPLEPAISVFPVTDPKDNPRIGSGWLDIYT 182

Query: 352 SKDEESKFNKTSASEQVMEELNRLINFFKEKGDREISLTITGHSLGGALSLLTAYEAGV- 411
           + D  S ++ TSA EQV  EL RL+  +K   D EIS+T TGHSLG  +S+L+A +    
Sbjct: 183 ASDSRSPYDTTSAQEQVQGELKRLLELYK---DEEISITFTGHSLGAVMSVLSAADLVYG 242

Query: 412 --------LFPTGVHISVVSFGAPRVGNLTFREKLNEM-GVKTLRVVIGQDIVPKLPGLF 471
                   L    V I+V +FG+PR+G+  F+  ++ +  +  LR+V   D+ P  P L 
Sbjct: 243 KKNNININLQKKQVPITVFAFGSPRIGDHNFKNVVDSLQPLNILRIVNVPDVAPHYPLL- 302

Query: 472 VNSIVNKLSLVTGKLNWVYRHVGKELRMDMYMSPYLKRESDMTGSHNLEIYLHLVDGFVS 531
                            +Y  +G+ L ++   S YLKR  +    HNLEIYLH + G   
Sbjct: 303 -----------------LYSEIGEVLEINTLNSTYLKRSLNFRNYHNLEIYLHGMAGMQD 362

Query: 532 RKGKFRWNSRRDVALVNKGSDMLVEELRIPQFWYQLPHKGLIKNRFGRW 561
             G F+    RD++LVNKG D L +E  +P  W  L +KG+++   G W
Sbjct: 363 TDGVFKLEIGRDISLVNKGLDALKDEYLVPSTWRCLANKGMLQMDDGTW 390

BLAST of Bhi08G001649 vs. TAIR10
Match: AT1G30370.1 (alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 590.5 bits (1521), Expect = 1.1e-168
Identity = 297/525 (56.57%), Postives = 390/525 (74.29%), Query Frame = 0

Query: 61  AMKRVTMLRNGTKKTKWYWKLKFGIRL--KAIRSAISSSLH---NQRRRLTCSTAIPTNT 120
           A+ +  + +   ++TK  W+LK  ++L  K+I+  + S L    + ++ L    +     
Sbjct: 4   ALVKTPLRKLRRRRTKRVWRLKQKLKLAWKSIKIRVKSHLPGFLSTKKHLFHIKSRKEEQ 63

Query: 121 PVNGIVSPLVVHQSRSSSKKNELRLAKPLASLLRMPLRAA-DFIDFGNHMTPTLSPRENI 180
            ++ +   +    + S+         K LA LL++P  +A DF+D G+ MTP  SPRE I
Sbjct: 64  DLSQVAQRICKISNDST---------KSLAFLLQLPKYSADDFLDRGDLMTPAASPREKI 123

Query: 181 SAVWRDLHGASDWDGLLDPLHPFLRREIVKYGEFAQATYDAFDFDPLSEFCGSCRYNRHK 240
           S +WR+LHG+++W+ LLDPLHP+LRRE+ KYGEF ++ YD+ DFDPLSEFCGS RYNR+K
Sbjct: 124 SKMWRELHGSNNWENLLDPLHPWLRREVTKYGEFVESVYDSLDFDPLSEFCGSSRYNRNK 183

Query: 241 LFNELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSTDEESDRI 300
           LF ELGL ++GYKVTKYIYA+S VD P WF SS +GE WS+DSNWMGFVAVS D ES RI
Sbjct: 184 LFEELGLTRHGYKVTKYIYAMSRVDVPQWFLSSALGETWSKDSNWMGFVAVSGDRESLRI 243

Query: 301 GRRDILVAWRGTVTPTEWYIDLKTKLKKIDRSDKK----VKVQRGFLTIYKSKDEESKFN 360
           GRRDI+VAWRGTVTPTEW++DL+T ++  D   K     VKVQ GFL+IY SK E +++N
Sbjct: 244 GRRDIVVAWRGTVTPTEWFMDLRTSMEPFDCEGKHGKTVVKVQSGFLSIYNSKSELTRYN 303

Query: 361 KTSASEQVMEELNRLINFFKEKGDREISLTITGHSLGGALSLLTAYEAGVLFPT-GVHIS 420
           K SASEQ M+E+ RL+NFFK++G+ E+SLTITGHSLGGAL+L+ AYEA    P    +IS
Sbjct: 304 KESASEQTMDEVKRLVNFFKDRGE-EVSLTITGHSLGGALALMNAYEAARDVPALSGNIS 363

Query: 421 VVSFGAPRVGNLTFREKLNEMGVKTLRVVIGQDIVPKLPGLFVNSIVNKLSLVTGKLNWV 480
           V+SFGAPRVGNL F+EKLN +GVK LRVV  QDIVPKLPG+  N ++NKL+ +T +LNWV
Sbjct: 364 VISFGAPRVGNLAFKEKLNSLGVKVLRVVNKQDIVPKLPGIVFNKVLNKLNPITSRLNWV 423

Query: 481 YRHVGKELRMDMYMSPYLKRESDMTGSHNLEIYLHLVDGFVSRKGKFRWNSRRDVALVNK 540
           YRHVG +L++D++ SPY+KR+SD+  +HNLE+YLH++DGF  +K  FR N+RRDVA VNK
Sbjct: 424 YRHVGTQLKLDVFSSPYVKRDSDLGRAHNLEVYLHVLDGFHRKKSGFRVNARRDVASVNK 483

Query: 541 GSDMLVEELRIPQFWYQLPHKGLIKNR-FGRWVKPGRNPEDIPSP 574
            +DML++ LRIP+FWYQ+ HKGLI N+  GRWVKP R PEDIPSP
Sbjct: 484 STDMLLDHLRIPEFWYQVAHKGLILNKQTGRWVKPVRAPEDIPSP 518

BLAST of Bhi08G001649 vs. TAIR10
Match: AT2G30550.2 (alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 395.2 bits (1014), Expect = 6.9e-110
Identity = 196/410 (47.80%), Postives = 276/410 (67.32%), Query Frame = 0

Query: 178 WRDLHGASDWDGLLDPLHPFLRREIVKYGEFAQATYDAFDFDPLSEFCGSCRYNRHKLFN 237
           WR + G  DW GL+DP+ P LR E+++YGE AQA YDAFDFDP S++CG+ R+ R + F+
Sbjct: 105 WRKIQGEDDWAGLMDPMDPILRSELIRYGEMAQACYDAFDFDPASKYCGTSRFTRLEFFD 164

Query: 238 ELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSTDEES-DRIGR 297
            LG+  +GY+V +Y+YA S ++ P++F  S+  +VWS+++NWMG+VAVS DE S +R+GR
Sbjct: 165 SLGMIDSGYEVARYLYATSNINLPNFFSKSRWSKVWSKNANWMGYVAVSDDETSRNRLGR 224

Query: 298 RDILVAWRGTVTPTEWYIDLKTKLK-----KIDRSDKKVKVQRGFLTIYKSKDEESKFNK 357
           RDI +AWRGTVT  EW  DLK  LK     KI   D  VKV+ GFL +Y  KD   KF +
Sbjct: 225 RDIAIAWRGTVTKLEWIADLKDYLKPVTENKIRCPDPAVKVESGFLDLYTDKDTTCKFAR 284

Query: 358 TSASEQVMEELNRLINFFKEKGDREISLTITGHSLGGALSLLTAYEAGVLFPTG------ 417
            SA EQ++ E+ RL+    +  D ++S+T+TGHSLGGAL++L+AY+   +          
Sbjct: 285 FSAREQILTEVKRLVEEHGDDDDSDLSITVTGHSLGGALAILSAYDIAEMRLNRSKKGKV 344

Query: 418 VHISVVSFGAPRVGNLTFREKLNEMGVKTLRVVIGQDIVPKLPGLFVN-SIVNKLSLVTG 477
           + ++V+++G PRVGN+ FRE++ E+GVK +RVV   D+VPK PGLF+N S  + L  +  
Sbjct: 345 IPVTVLTYGGPRVGNVRFRERMEELGVKVMRVVNVHDVVPKSPGLFLNESRPHALMKIAE 404

Query: 478 KLNWVYRHVGKELRMDMYMSPYLKRESDMTGSHNLEIYLHLVDGFVSRKGKFRWNSRRDV 537
            L W Y HVG+EL +D   SP+LK   D++ +HNLE  LHL+DG+  +  +F  +S RD 
Sbjct: 405 GLPWCYSHVGEELALDHQNSPFLKPSVDVSTAHNLEAMLHLLDGYHGKGERFVLSSGRDH 464

Query: 538 ALVNKGSDMLVEELRIPQFWYQLPHKGLIKNRFGRWVKPGR-NPEDIPSP 574
           ALVNK SD L E L+IP FW Q  +KG+++N  GRW++  R   ED  SP
Sbjct: 465 ALVNKASDFLKEHLQIPPFWRQDANKGMVRNSEGRWIQAERLRFEDHHSP 514

BLAST of Bhi08G001649 vs. TAIR10
Match: AT1G06800.1 (alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 389.8 bits (1000), Expect = 2.9e-108
Identity = 185/402 (46.02%), Postives = 265/402 (65.92%), Query Frame = 0

Query: 178 WRDLHGASDWDGLLDPLHPFLRREIVKYGEFAQATYDAFDFDPLSEFCGSCRYNRHKLFN 237
           WR + G  DW GL+DP+ P LR E+++YGE AQA YDAFDFDP S +CGSCR+ R  LF+
Sbjct: 89  WRKIQGEDDWAGLMDPMDPVLRSELIRYGEMAQACYDAFDFDPFSRYCGSCRFTRRHLFD 148

Query: 238 ELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSTDEESD--RIG 297
            LG+  +GY+V +Y+YA S ++ P++F  S+  +VWS+++NWMG+VAVS D E+   R+G
Sbjct: 149 SLGIIDSGYEVARYLYATSNINLPNFFSKSRWSKVWSKNANWMGYVAVSDDNEATRCRLG 208

Query: 298 RRDILVAWRGTVTPTEWYIDLKTKLKKIDRS-----DKKVKVQRGFLTIYKSKDEESKFN 357
           RRDI +AWRGTVT  EW  DLK  LK +  +     D  VK + GFL +Y  KD    F+
Sbjct: 209 RRDIAIAWRGTVTRLEWIADLKDFLKPVSGNGFRCPDPAVKAESGFLDLYTDKDTSCNFS 268

Query: 358 KTSASEQVMEELNRLINFFKEKGDREISLTITGHSLGGALSLLTAYEAGVLFPTG----- 417
           K SA EQV+ E+ RL+  + ++   E+S+T+TGHSLGGAL++L+AY+   +         
Sbjct: 269 KFSAREQVLTEVKRLVERYGDEEGEELSITVTGHSLGGALAVLSAYDVAEMGVNRTRKGK 328

Query: 418 -VHISVVSFGAPRVGNLTFREKLNEMGVKTLRVVIGQDIVPKLPGLFVNS-IVNKLSLVT 477
            + ++  ++G PRVGN+ F+E++ ++GVK LRVV   D+V K PGLF+N      L  + 
Sbjct: 329 VIPVTAFTYGGPRVGNIRFKERIEKLGVKVLRVVNEHDVVAKSPGLFLNERAPQALMKLA 388

Query: 478 GKLNWVYRHVGKELRMDMYMSPYLKRESDMTGSHNLEIYLHLVDGFVSRKGKFRWNSRRD 537
           G L W Y HVG+ L +D   SP+LK   D++ +HNLE  LHL+DG+  +  +F  +S RD
Sbjct: 389 GGLPWCYSHVGEMLPLDHQKSPFLKPTVDLSTAHNLEALLHLLDGYHGKGQRFVLSSGRD 448

Query: 538 VALVNKGSDMLVEELRIPQFWYQLPHKGLIKNRFGRWVKPGR 566
            ALVNK SD L +   +P +W Q  +KG+++N  GRW++P R
Sbjct: 449 PALVNKASDFLKDHFMVPPYWRQDANKGMVRNTDGRWIQPDR 490

BLAST of Bhi08G001649 vs. TAIR10
Match: AT1G51440.1 (alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 369.0 bits (946), Expect = 5.3e-102
Identity = 192/423 (45.39%), Postives = 269/423 (63.59%), Query Frame = 0

Query: 177 VWRDLHGASDWDGLLDPLHPFLRREIVKYGEFAQATYDAFDFDPLSEFCGSCRYNRHKLF 236
           +WR++ G ++W+G LDP++  LRREI++YGEFAQA YD+FDFDP S++CGSC+Y+    F
Sbjct: 90  IWREVQGCNNWEGQLDPMNNHLRREIIRYGEFAQACYDSFDFDPHSKYCGSCKYHPSDFF 149

Query: 237 NELGL-AQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSTDEES-DRI 296
             L L    GY +T+Y+YA S ++ P++F+ SK+  +WS+ +NWMGFVAV+TDEE   R+
Sbjct: 150 LNLDLHLHKGYTITRYLYATSNINLPNFFQKSKLSSIWSQHANWMGFVAVATDEEEVSRL 209

Query: 297 GRRDILVAWRGTVTPTEWYIDLKTKLKKID-RSDKKVKVQRGFLTIYKSKDEESKFNKTS 356
           GRRDI++AWRGTVT  EW  DLK  L   +   D  +K++ GF  +Y  K++  KF+  S
Sbjct: 210 GRRDIVIAWRGTVTYLEWIYDLKDILCSANFGDDPSIKIELGFHDLYTKKEDSCKFSSFS 269

Query: 357 ASEQVMEELNRLINFF-KEKGDREISLTITGHSLGGALSLLTAYEAGVL----FPTG--- 416
           A EQV+ E+ RLI ++  E+   + S+T+TGHSLG +L+L++AY+   L     P     
Sbjct: 270 AREQVLAEVKRLIEYYGTEEEGHKTSITVTGHSLGASLALVSAYDIAELNLNHVPENNYK 329

Query: 417 VHISVVSFGAPRVGNLTFREKLNEMGVKTLRVVIGQDIVPKLPGLFVNSIVNKLSLVTGK 476
           + I+V SF  PRVGNL F+E+ +E+GVK LRVV   D VP +PG+F N        V  K
Sbjct: 330 IPITVFSFSGPRVGNLRFKERCDELGVKVLRVVNVHDKVPSVPGIFTNEKFQFQKYVEEK 389

Query: 477 LN--WVYRHVGKELRMDMYMSPYLKRESDMTGSHNLEIYLHLVDGFVSR----KGKFRWN 536
            +  W Y HVG EL +D   SP+LK   D+  +HNLE  LHLVDG+  +    + +F   
Sbjct: 390 TSFPWSYAHVGVELALDHKKSPFLKPTKDLGCAHNLEALLHLVDGYHGKDEEAEKRFCLV 449

Query: 537 SRRDVALVNKGSDMLVEELRIPQFWYQLPHKGLIKNRFGRWVKPGR------NPEDIPSP 577
           ++RD+ALVNK  D L  E  +P  W Q  +KG++KN  G+WV P R       PEDI   
Sbjct: 450 TKRDIALVNKSCDFLRGEYHVPPCWRQDENKGMVKNGDGQWVLPDRPLLEPHGPEDIAHH 509

BLAST of Bhi08G001649 vs. TAIR10
Match: AT1G06250.1 (alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 263.8 bits (673), Expect = 2.4e-70
Identity = 155/409 (37.90%), Postives = 223/409 (54.52%), Query Frame = 0

Query: 172 ENISAVWRDLHGASDWDGLLDPLHPFLRREIVKYGEFAQATYDAFDFDPLSEFCGSCRYN 231
           E I   W+ L G + W GLLDPL P LRR I+ YGE +Q  YDAF++D  S + G C Y+
Sbjct: 3   EGIPKRWKVLSGQNKWKGLLDPLDPDLRRYIIHYGEMSQVGYDAFNWDRKSRYAGDCYYS 62

Query: 232 RHKLFNELGLAQNG---YKVTKYIYALSPVDGPDWFESSKIGEVWSR-DSNWMGFVAVST 291
           +++L    G  +     YKVTKYIYA + +  P  F    + +  SR  +NWMG++AV+T
Sbjct: 63  KNRLLARTGFLKANPFRYKVTKYIYATASIKLPISFIVKSLSKDASRVQTNWMGYIAVAT 122

Query: 292 DEESDRIGRRDILVAWRGTVTPTEWYID----LKTKLKKIDRSDKK--VKVQRGFLTIYK 351
           D+    +GRRDI+VAWRGT+ P EW  D    L+  +     +D K   ++  G+L IY 
Sbjct: 123 DQGKAMLGRRDIVVAWRGTLQPYEWANDFDFPLEPAISVFPVTDPKDNPRIGSGWLDIYT 182

Query: 352 SKDEESKFNKTSASEQVMEELNRLINFFKEKGDREISLTITGHSLGGALSLLTAYEAGV- 411
           + D  S ++ TSA EQV  EL RL+  +K   D EIS+T TGHSLG  +S+L+A +    
Sbjct: 183 ASDSRSPYDTTSAQEQVQGELKRLLELYK---DEEISITFTGHSLGAVMSVLSAADLVYG 242

Query: 412 --------LFPTGVHISVVSFGAPRVGNLTFREKLNEM-GVKTLRVVIGQDIVPKLPGLF 471
                   L    V I+V +FG+PR+G+  F+  ++ +  +  LR+V   D+ P  P L 
Sbjct: 243 KKNNININLQKKQVPITVFAFGSPRIGDHNFKNVVDSLQPLNILRIVNVPDVAPHYPLL- 302

Query: 472 VNSIVNKLSLVTGKLNWVYRHVGKELRMDMYMSPYLKRESDMTGSHNLEIYLHLVDGFVS 531
                            +Y  +G+ L ++   S YLKR  +    HNLEIYLH + G   
Sbjct: 303 -----------------LYSEIGEVLEINTLNSTYLKRSLNFRNYHNLEIYLHGMAGMQD 362

Query: 532 RKGKFRWNSRRDVALVNKGSDMLVEELRIPQFWYQLPHKGLIKNRFGRW 561
             G F+    RD++LVNKG D L +E  +P  W  L +KG+++   G W
Sbjct: 363 TDGVFKLEIGRDISLVNKGLDALKDEYLVPSTWRCLANKGMLQMDDGTW 390

BLAST of Bhi08G001649 vs. TrEMBL
Match: tr|A0A1S3BBX3|A0A1S3BBX3_CUCME (phospholipase A1-Igamma1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103487991 PE=4 SV=1)

HSP 1 Score: 999.6 bits (2583), Expect = 2.9e-288
Identity = 485/536 (90.49%), Postives = 512/536 (95.52%), Query Frame = 0

Query: 50  MEAKMATPRGVAMKRVTMLRNGTKKTKWYWKLKFGIRLKAIRSAISSSLHNQRRRLTCST 109
           MEAK+ATPRG+ +KR TMLRNGTKKTKWYWKLK GIR +AI++A+SSSLHNQRRRLTCST
Sbjct: 1   MEAKLATPRGIPVKRTTMLRNGTKKTKWYWKLKLGIRFRAIKAALSSSLHNQRRRLTCST 60

Query: 110 AIPTN-----TPVNGIVSPLVVHQSRSSSKKNELRLAKPLASLLRMPLRAADFIDFGNHM 169
           AI T      TP NG+VSPLV+HQS+  SKKN+LRLAKPLASLLRMPLRAADFIDFGNHM
Sbjct: 61  AITTTTTPQLTPTNGLVSPLVIHQSK-PSKKNDLRLAKPLASLLRMPLRAADFIDFGNHM 120

Query: 170 TPTLSPRENISAVWRDLHGASDWDGLLDPLHPFLRREIVKYGEFAQATYDAFDFDPLSEF 229
           TPTLSPR+NISAVWRDLHGASDW+GLLDPLHPFLRREIVKYGEFAQATYDAFDFDPLSEF
Sbjct: 121 TPTLSPRDNISAVWRDLHGASDWEGLLDPLHPFLRREIVKYGEFAQATYDAFDFDPLSEF 180

Query: 230 CGSCRYNRHKLFNELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVA 289
           CGSCRYNRHKL NELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMG+VA
Sbjct: 181 CGSCRYNRHKLLNELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGYVA 240

Query: 290 VSTDEESDRIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRSDKKVKVQRGFLTIYKSKD 349
           VS+DEESDRIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRS+KKVKVQRGFLTIYKSKD
Sbjct: 241 VSSDEESDRIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRSNKKVKVQRGFLTIYKSKD 300

Query: 350 EESKFNKTSASEQVMEELNRLINFFKEKGDREISLTITGHSLGGALSLLTAYEAGVLFPT 409
           E+SKFNKTSASEQVMEEL+RLI+FFKEKGDREISLTITGHSLGGALSLLTAYEAGV FP 
Sbjct: 301 EDSKFNKTSASEQVMEELHRLIDFFKEKGDREISLTITGHSLGGALSLLTAYEAGVTFPA 360

Query: 410 GVHISVVSFGAPRVGNLTFREKLNEMGVKTLRVVIGQDIVPKLPGLFVNSIVNKLSLVTG 469
            VH+SVVSFGAPRVGNL FREKLNEMGVKTLRVVI QDIVPKLPGLFVNSIVNKL  VTG
Sbjct: 361 DVHVSVVSFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVNKLGAVTG 420

Query: 470 KLNWVYRHVGKELRMDMYMSPYLKRESDMTGSHNLEIYLHLVDGFVSRKGKFRWNSRRDV 529
           KLNWVYRHVGKELRM+MYMSPYLK++SDM+GSHNLEIYLHLVDGFV++KGKFRWNSRRDV
Sbjct: 421 KLNWVYRHVGKELRMNMYMSPYLKKDSDMSGSHNLEIYLHLVDGFVAKKGKFRWNSRRDV 480

Query: 530 ALVNKGSDMLVEELRIPQFWYQLPHKGLIKNRFGRWVKPGRNPEDIPSPFSQPSHV 581
           ALVNKGSDMLVEELRIP+FWYQLPHKGLIKNRFGRWVKPGRNPEDIPSPFSQP  V
Sbjct: 481 ALVNKGSDMLVEELRIPEFWYQLPHKGLIKNRFGRWVKPGRNPEDIPSPFSQPLQV 535

BLAST of Bhi08G001649 vs. TrEMBL
Match: tr|A0A0A0M111|A0A0A0M111_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G537320 PE=4 SV=1)

HSP 1 Score: 991.9 bits (2563), Expect = 6.1e-286
Identity = 481/535 (89.91%), Postives = 511/535 (95.51%), Query Frame = 0

Query: 50  MEAKMATPRGVAMKRVTMLRNGTKKTKWYWKLKFGIRLKAIRSAISSSLHNQRRRLTCST 109
           ME K ATPRG+ +KR TMLRNGTKKTKWYWKLK GI+ KAI++A+SSSLHNQRRRLTCST
Sbjct: 1   MEPKFATPRGIPVKRTTMLRNGTKKTKWYWKLKLGIQFKAIKAALSSSLHNQRRRLTCST 60

Query: 110 AI----PTNTPVNGIVSPLVVHQSRSSSKKNELRLAKPLASLLRMPLRAADFIDFGNHMT 169
           AI    P +TP NG+VSPLV+HQS+ SSKKN+LRLAKPLASLLRMPLRA+DFIDFGNHMT
Sbjct: 61  AITTTTPQHTPTNGLVSPLVIHQSK-SSKKNDLRLAKPLASLLRMPLRASDFIDFGNHMT 120

Query: 170 PTLSPRENISAVWRDLHGASDWDGLLDPLHPFLRREIVKYGEFAQATYDAFDFDPLSEFC 229
           PTLSPR+ ISAVWRDLHGASDW+GLLDPLHPFLRREIVKYGEFAQATYDAFDFDPLSEFC
Sbjct: 121 PTLSPRDKISAVWRDLHGASDWEGLLDPLHPFLRREIVKYGEFAQATYDAFDFDPLSEFC 180

Query: 230 GSCRYNRHKLFNELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAV 289
           GSCRYNR KL NELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAV
Sbjct: 181 GSCRYNRQKLLNELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAV 240

Query: 290 STDEESDRIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRSDKKVKVQRGFLTIYKSKDE 349
           S+DEESDRIGRRDILV+WRGTVTPTEWYIDLKTKLKKIDRS+KKVKVQRGFLTIYKSKDE
Sbjct: 241 SSDEESDRIGRRDILVSWRGTVTPTEWYIDLKTKLKKIDRSNKKVKVQRGFLTIYKSKDE 300

Query: 350 ESKFNKTSASEQVMEELNRLINFFKEKGDREISLTITGHSLGGALSLLTAYEAGVLFPTG 409
           +SKFNKTSASEQVMEEL+RLI+FFK+KGDREISLTITGHSLGGALSLLTAYEAGV FP  
Sbjct: 301 DSKFNKTSASEQVMEELHRLIDFFKQKGDREISLTITGHSLGGALSLLTAYEAGVTFPAD 360

Query: 410 VHISVVSFGAPRVGNLTFREKLNEMGVKTLRVVIGQDIVPKLPGLFVNSIVNKLSLVTGK 469
           VH+SVVSFGAPRVGNL FREKLNEMGVK LRVVIGQDIVPKLPGLFVNSIVNKLS VTGK
Sbjct: 361 VHVSVVSFGAPRVGNLAFREKLNEMGVKILRVVIGQDIVPKLPGLFVNSIVNKLSAVTGK 420

Query: 470 LNWVYRHVGKELRMDMYMSPYLKRESDMTGSHNLEIYLHLVDGFVSRKGKFRWNSRRDVA 529
           LNWVYRHVGKELRM+M+MSPYLK++SDM+GSHNLEIYLHLVDGFV++KGKFRWNSRRDVA
Sbjct: 421 LNWVYRHVGKELRMNMFMSPYLKKDSDMSGSHNLEIYLHLVDGFVTKKGKFRWNSRRDVA 480

Query: 530 LVNKGSDMLVEELRIPQFWYQLPHKGLIKNRFGRWVKPGRNPEDIPSPFSQPSHV 581
           LVNKGSDMLVEELRIP+FWYQLPHKGLIKNRFGRWVKPGRN EDIPSPFSQPS V
Sbjct: 481 LVNKGSDMLVEELRIPEFWYQLPHKGLIKNRFGRWVKPGRNVEDIPSPFSQPSQV 534

BLAST of Bhi08G001649 vs. TrEMBL
Match: tr|A0A2P4HZ01|A0A2P4HZ01_QUESU (Phospholipase a1-igamma2, chloroplastic OS=Quercus suber OX=58331 GN=CFP56_46530 PE=4 SV=1)

HSP 1 Score: 695.7 bits (1794), Expect = 9.1e-197
Identity = 336/518 (64.86%), Postives = 408/518 (78.76%), Query Frame = 0

Query: 60  VAMKRVTMLRNGTKKTKWYWKLKFGIRLKAIRSAISSSLHNQRRRLTCSTAIP----TNT 119
           V+  R      G KKTKW  KLKFG+  KA++ A SS++   R  LTC++++        
Sbjct: 3   VSPLRQVRKEKGAKKTKWVLKLKFGVTWKALKKASSSTMKLNRFHLTCASSMKKLSLVRV 62

Query: 120 PVNGIVSPLVVHQSRSSSKKNELRLAKPLASLLRMPLRAADFIDFGNHMTPTLSPRENIS 179
                V P+   Q   + KK    + K L SLL++P  A+DFID+G+ MTPT SP+ENIS
Sbjct: 63  QAEEAVKPI---QQAHTKKKASHVVTKSLLSLLQLPYTASDFIDWGDLMTPTKSPKENIS 122

Query: 180 AVWRDLHGASDWDGLLDPLHPFLRREIVKYGEFAQATYDAFDFDPLSEFCGSCRYNRHKL 239
             WR++HG  +WD LLDPLHP+LRREI+KYGEFAQATYD FDFDPLSEFCGSCRYNRHKL
Sbjct: 123 TKWREIHGLHNWDSLLDPLHPWLRREIIKYGEFAQATYDGFDFDPLSEFCGSCRYNRHKL 182

Query: 240 FNELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSTDEESDRIG 299
           F ELGL  +GYKVT+YIYA+S VD P WFE S +GE WS+DSNWMG+VAVS DEES+RIG
Sbjct: 183 FEELGLTNHGYKVTEYIYAMSHVDVPSWFERSHLGETWSKDSNWMGYVAVSNDEESERIG 242

Query: 300 RRDILVAWRGTVTPTEWYIDLKTKLKKIDRSDKKVKVQRGFLTIYKSKDEESKFNKTSAS 359
           RRDI++AWRGT+ PTEW+ DLK  L++I   D K++VQ GF +IYKSK E +++NK+SAS
Sbjct: 243 RRDIVMAWRGTIAPTEWFTDLKAMLERI--GDGKIEVQHGFRSIYKSKSEFTRYNKSSAS 302

Query: 360 EQVMEELNRLINFFKEKGDREISLTITGHSLGGALSLLTAYEAGVLFPTGVHISVVSFGA 419
           EQVMEE+NRLI F+K K D EIS TITGHSLGGAL+LL AYEA    P G+ ISV+SFGA
Sbjct: 303 EQVMEEVNRLIKFYKAK-DEEISFTITGHSLGGALALLNAYEAATSIP-GLPISVISFGA 362

Query: 420 PRVGNLTFREKLNEMGVKTLRVVIGQDIVPKLPGLFVNSIVNKLSLVTGKLNWVYRHVGK 479
           PRVGNL F+EKL+EMGVKTLR+V+ QDIVPK PG   N+I++KL+ VT K NWVYRHVG 
Sbjct: 363 PRVGNLAFKEKLDEMGVKTLRIVVKQDIVPKFPGFIFNNILHKLNFVTKKFNWVYRHVGS 422

Query: 480 ELRMDMYMSPYLKRESDMTGSHNLEIYLHLVDGFVSRKGKFRWNSRRDVALVNKGSDMLV 539
           +L++DM+MSPYLKRESD+ GSHNLEIYLHL+DGF+S + KFRWN+RRDVALVNK +DML+
Sbjct: 423 QLKLDMFMSPYLKRESDLIGSHNLEIYLHLLDGFLSERRKFRWNARRDVALVNKTTDMLI 482

Query: 540 EELRIPQFWYQLPHKGLIKNRFGRWVKPGRNPEDIPSP 574
           EELRIP+FWYQLP+KGL+ N+ GRWVKP R PEDIPSP
Sbjct: 483 EELRIPKFWYQLPYKGLVLNKHGRWVKPSREPEDIPSP 513

BLAST of Bhi08G001649 vs. TrEMBL
Match: tr|A0A2N9FVF1|A0A2N9FVF1_FAGSY (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS18783 PE=4 SV=1)

HSP 1 Score: 680.2 bits (1754), Expect = 3.9e-192
Identity = 327/517 (63.25%), Postives = 405/517 (78.34%), Query Frame = 0

Query: 62  MKRVTMLRNGTKKTKWYWKLKFGIRLKAIRSAISSSLHNQRRRLTCSTAIPTNTPVNGIV 121
           +++VTM   G KKTKW  KLKFG+  +AIR A SS++   R  LTC+++I   +PV    
Sbjct: 6   LRQVTM-EKGAKKTKWVLKLKFGVTWEAIRKAASSTMKRHRFHLTCASSIKQLSPVRVHQ 65

Query: 122 SPLVVHQSRSSSKKNELRLAKPLASLLRMPLRAADFIDFGNHMTPTLSPRENISAVWRDL 181
           +  +    R+ +KKN     K LA LL +P  A+DFID G+ MTPT S +ENISA W ++
Sbjct: 66  AAALKPIPRAKTKKNVRNGTKSLAYLLNLPYTASDFIDRGDQMTPTKSQKENISAKWHEI 125

Query: 182 HGASDWDGLLDPLHPFLRREIVKYGEFAQATYDAFDFDPLSEFCGSCRYNRHKLFNELGL 241
            G  +W+ L+DPL P+LR E++KYGEF QATYDAFDFDPLSE+CGSCRYNRHKLF ELGL
Sbjct: 126 QGLLNWESLIDPLQPWLRCEVIKYGEFVQATYDAFDFDPLSEYCGSCRYNRHKLFEELGL 185

Query: 242 AQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSTDEESDRIGRRDILV 301
            +NGYKVTKYIYA+S VD P W E S +GE WS+DSNWMG+VAVS +EES RIGRRDI++
Sbjct: 186 TKNGYKVTKYIYAMSHVDVPQWLERSHLGETWSKDSNWMGYVAVSNNEESKRIGRRDIVM 245

Query: 302 AWRGTVTPTEWYIDLKTKLKKIDRSDKKVKVQRGFLTIYKSKDEESKFNKTSASEQVMEE 361
           AWRGTV PTEW+ DLK KL+ I   +  +KVQ GFL+IYK K E +++NK SASEQVM+E
Sbjct: 246 AWRGTVAPTEWFTDLKAKLESIGNGN--IKVQHGFLSIYKKKSERTRYNKLSASEQVMQE 305

Query: 362 LNRLINFFKEKGDREISLTITGHSLGGALSLLTAYEAGVLFPTGVHISVVSFGAPRVGNL 421
           +NRLI F+K     EIS TITGHSLGGAL+LL AYEA  L P G+ ISV+SFGAPRVGNL
Sbjct: 306 VNRLIEFYK---GEEISFTITGHSLGGALALLNAYEAATLIP-GLPISVISFGAPRVGNL 365

Query: 422 TFREKLNEMGVKTLRVVIGQDIVPKLPGLFVNSIVNKLSLVTGKLNWVYRHVGKELRMDM 481
            F++KL EM VKTLR+V+ QDIVPK+PG+  N+I++KL+ VT K NWVYRHVG EL++D+
Sbjct: 366 AFKDKLTEMKVKTLRIVVKQDIVPKVPGIIFNNILHKLNFVTQKFNWVYRHVGTELKLDV 425

Query: 482 YMSPYLKRESDMTGSHNLEIYLHLVDGFVSRKGKFRWNSRRDVALVNKGSDMLVEELRIP 541
           + SPYLK ESD+ G HNLE+YLHL+DGF+S++ KFRWN+RRDVALVNK SDML+EELRIP
Sbjct: 426 FTSPYLKHESDLLGCHNLELYLHLLDGFLSKQSKFRWNARRDVALVNKTSDMLIEELRIP 485

Query: 542 QFWYQLPHKGLIKNRFGRWVKPGRNPEDIPSPFSQPS 579
           +FWY+LP+KGL+ N++GRWVKP R  EDIPSPFS  S
Sbjct: 486 EFWYELPYKGLVLNKYGRWVKPSREAEDIPSPFSVAS 515

BLAST of Bhi08G001649 vs. TrEMBL
Match: tr|A0A2C9U5J1|A0A2C9U5J1_MANES (Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_17G074500 PE=4 SV=1)

HSP 1 Score: 666.0 bits (1717), Expect = 7.7e-188
Identity = 321/518 (61.97%), Postives = 407/518 (78.57%), Query Frame = 0

Query: 58  RGVAMKRVTMLRNGTKKTKWYWKLKFGIRLKAIRSAISSSLHNQRRRLTCSTAIPTNTPV 117
           R VA K+ TM R  T+     W+LK  +  KAI+ A +S     R RL+C  ++      
Sbjct: 8   RHVATKK-TMSRKATR----VWRLKLSVTWKAIKRAFTSKF---RLRLSCVRSMKP---- 67

Query: 118 NGIVSPLVVHQSRSSSKKNELRLAKPLASLLRMPLRAADFIDFGNHMTPTLSPRENISAV 177
              +    +HQ  + +KKN  R  + L  LL +P  A DFID G+ MTPTLSP+EN+S  
Sbjct: 68  ---LPTAKIHQ-ETKTKKNGCRPRRTLEHLLNVPYTALDFIDRGDCMTPTLSPKENLSTR 127

Query: 178 WRDLHGASDWDGLLDPLHPFLRREIVKYGEFAQATYDAFDFDPLSEFCGSCRYNRHKLFN 237
           WR++HG+++W  LLDPLHP+LRREIVKYGE  +ATYDAFDFDPLSE+CGSCRYNRHK F 
Sbjct: 128 WREIHGSNNWGNLLDPLHPWLRREIVKYGELVEATYDAFDFDPLSEYCGSCRYNRHKFFQ 187

Query: 238 ELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSTDEESDRIGRR 297
           ELGL ++GY+VTKY+YA+S VD P+WF  S    VWS+DSNWMG+VAVS +EES RIGRR
Sbjct: 188 ELGLIKHGYEVTKYLYAMSHVDVPEWFAGS--NSVWSKDSNWMGYVAVSNEEESRRIGRR 247

Query: 298 DILVAWRGTVTPTEWYIDLKTKLKKIDRSDKKVKVQRGFLTIYKSKDEESKFNKTSASEQ 357
           DI+VAWRGTV PTEW+ DLK KL++ D     +KVQ GFL+IYKSKDE +++NK+SASEQ
Sbjct: 248 DIVVAWRGTVAPTEWFTDLKAKLEEFDDEKDNIKVQCGFLSIYKSKDELTRYNKSSASEQ 307

Query: 358 VMEELNRLINFFKEKGDREISLTITGHSLGGALSLLTAYEAGVLFPTGVHISVVSFGAPR 417
           VMEEL +L+NF++E G+ E+SLT+TGHSLGGAL+LL AYEA +  P  + ISV+SFGAPR
Sbjct: 308 VMEELKKLVNFYRENGE-EVSLTLTGHSLGGALALLNAYEAAISIP-NLFISVISFGAPR 367

Query: 418 VGNLTFREKLNEMGVKTLRVVIGQDIVPKLPGLFVNSIVNKLSLVTGKLNWVYRHVGKEL 477
           VGNL F+EKLNE+GVKTLR+V+ QDIVPK+PG+ VN I+NK++ +T +LNWVYRHVG +L
Sbjct: 368 VGNLAFKEKLNELGVKTLRIVVKQDIVPKVPGIIVNKIINKINSLTQRLNWVYRHVGTQL 427

Query: 478 RMDMYMSPYLKRESDMTGSHNLEIYLHLVDGFVSRKGKFRWNSRRDVALVNKGSDMLVEE 537
           ++D++MSPYLK ESD +G HNLE+YLHL+DGF+ +K K+RWN+RRD+ALVNK SDML+EE
Sbjct: 428 KLDVFMSPYLKHESDWSGCHNLEVYLHLLDGFLGKKLKYRWNARRDLALVNKSSDMLIEE 487

Query: 538 LRIPQFWYQLPHKGLIKNRFGRWVKPGRNPEDIPSPFS 576
           L+IP+FW+QLPHKGL+ N+ GRWVKP R PEDIPSPFS
Sbjct: 488 LKIPEFWFQLPHKGLVLNKHGRWVKPSREPEDIPSPFS 505

BLAST of Bhi08G001649 vs. NCBI nr
Match: XP_008444745.1 (PREDICTED: phospholipase A1-Igamma1, chloroplastic [Cucumis melo])

HSP 1 Score: 999.6 bits (2583), Expect = 4.4e-288
Identity = 485/536 (90.49%), Postives = 512/536 (95.52%), Query Frame = 0

Query: 50  MEAKMATPRGVAMKRVTMLRNGTKKTKWYWKLKFGIRLKAIRSAISSSLHNQRRRLTCST 109
           MEAK+ATPRG+ +KR TMLRNGTKKTKWYWKLK GIR +AI++A+SSSLHNQRRRLTCST
Sbjct: 1   MEAKLATPRGIPVKRTTMLRNGTKKTKWYWKLKLGIRFRAIKAALSSSLHNQRRRLTCST 60

Query: 110 AIPTN-----TPVNGIVSPLVVHQSRSSSKKNELRLAKPLASLLRMPLRAADFIDFGNHM 169
           AI T      TP NG+VSPLV+HQS+  SKKN+LRLAKPLASLLRMPLRAADFIDFGNHM
Sbjct: 61  AITTTTTPQLTPTNGLVSPLVIHQSK-PSKKNDLRLAKPLASLLRMPLRAADFIDFGNHM 120

Query: 170 TPTLSPRENISAVWRDLHGASDWDGLLDPLHPFLRREIVKYGEFAQATYDAFDFDPLSEF 229
           TPTLSPR+NISAVWRDLHGASDW+GLLDPLHPFLRREIVKYGEFAQATYDAFDFDPLSEF
Sbjct: 121 TPTLSPRDNISAVWRDLHGASDWEGLLDPLHPFLRREIVKYGEFAQATYDAFDFDPLSEF 180

Query: 230 CGSCRYNRHKLFNELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVA 289
           CGSCRYNRHKL NELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMG+VA
Sbjct: 181 CGSCRYNRHKLLNELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGYVA 240

Query: 290 VSTDEESDRIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRSDKKVKVQRGFLTIYKSKD 349
           VS+DEESDRIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRS+KKVKVQRGFLTIYKSKD
Sbjct: 241 VSSDEESDRIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRSNKKVKVQRGFLTIYKSKD 300

Query: 350 EESKFNKTSASEQVMEELNRLINFFKEKGDREISLTITGHSLGGALSLLTAYEAGVLFPT 409
           E+SKFNKTSASEQVMEEL+RLI+FFKEKGDREISLTITGHSLGGALSLLTAYEAGV FP 
Sbjct: 301 EDSKFNKTSASEQVMEELHRLIDFFKEKGDREISLTITGHSLGGALSLLTAYEAGVTFPA 360

Query: 410 GVHISVVSFGAPRVGNLTFREKLNEMGVKTLRVVIGQDIVPKLPGLFVNSIVNKLSLVTG 469
            VH+SVVSFGAPRVGNL FREKLNEMGVKTLRVVI QDIVPKLPGLFVNSIVNKL  VTG
Sbjct: 361 DVHVSVVSFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVNKLGAVTG 420

Query: 470 KLNWVYRHVGKELRMDMYMSPYLKRESDMTGSHNLEIYLHLVDGFVSRKGKFRWNSRRDV 529
           KLNWVYRHVGKELRM+MYMSPYLK++SDM+GSHNLEIYLHLVDGFV++KGKFRWNSRRDV
Sbjct: 421 KLNWVYRHVGKELRMNMYMSPYLKKDSDMSGSHNLEIYLHLVDGFVAKKGKFRWNSRRDV 480

Query: 530 ALVNKGSDMLVEELRIPQFWYQLPHKGLIKNRFGRWVKPGRNPEDIPSPFSQPSHV 581
           ALVNKGSDMLVEELRIP+FWYQLPHKGLIKNRFGRWVKPGRNPEDIPSPFSQP  V
Sbjct: 481 ALVNKGSDMLVEELRIPEFWYQLPHKGLIKNRFGRWVKPGRNPEDIPSPFSQPLQV 535

BLAST of Bhi08G001649 vs. NCBI nr
Match: XP_004135535.1 (PREDICTED: phospholipase A1-Igamma1, chloroplastic [Cucumis sativus] >KGN65881.1 hypothetical protein Csa_1G537320 [Cucumis sativus])

HSP 1 Score: 991.9 bits (2563), Expect = 9.3e-286
Identity = 481/535 (89.91%), Postives = 511/535 (95.51%), Query Frame = 0

Query: 50  MEAKMATPRGVAMKRVTMLRNGTKKTKWYWKLKFGIRLKAIRSAISSSLHNQRRRLTCST 109
           ME K ATPRG+ +KR TMLRNGTKKTKWYWKLK GI+ KAI++A+SSSLHNQRRRLTCST
Sbjct: 1   MEPKFATPRGIPVKRTTMLRNGTKKTKWYWKLKLGIQFKAIKAALSSSLHNQRRRLTCST 60

Query: 110 AI----PTNTPVNGIVSPLVVHQSRSSSKKNELRLAKPLASLLRMPLRAADFIDFGNHMT 169
           AI    P +TP NG+VSPLV+HQS+ SSKKN+LRLAKPLASLLRMPLRA+DFIDFGNHMT
Sbjct: 61  AITTTTPQHTPTNGLVSPLVIHQSK-SSKKNDLRLAKPLASLLRMPLRASDFIDFGNHMT 120

Query: 170 PTLSPRENISAVWRDLHGASDWDGLLDPLHPFLRREIVKYGEFAQATYDAFDFDPLSEFC 229
           PTLSPR+ ISAVWRDLHGASDW+GLLDPLHPFLRREIVKYGEFAQATYDAFDFDPLSEFC
Sbjct: 121 PTLSPRDKISAVWRDLHGASDWEGLLDPLHPFLRREIVKYGEFAQATYDAFDFDPLSEFC 180

Query: 230 GSCRYNRHKLFNELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAV 289
           GSCRYNR KL NELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAV
Sbjct: 181 GSCRYNRQKLLNELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAV 240

Query: 290 STDEESDRIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRSDKKVKVQRGFLTIYKSKDE 349
           S+DEESDRIGRRDILV+WRGTVTPTEWYIDLKTKLKKIDRS+KKVKVQRGFLTIYKSKDE
Sbjct: 241 SSDEESDRIGRRDILVSWRGTVTPTEWYIDLKTKLKKIDRSNKKVKVQRGFLTIYKSKDE 300

Query: 350 ESKFNKTSASEQVMEELNRLINFFKEKGDREISLTITGHSLGGALSLLTAYEAGVLFPTG 409
           +SKFNKTSASEQVMEEL+RLI+FFK+KGDREISLTITGHSLGGALSLLTAYEAGV FP  
Sbjct: 301 DSKFNKTSASEQVMEELHRLIDFFKQKGDREISLTITGHSLGGALSLLTAYEAGVTFPAD 360

Query: 410 VHISVVSFGAPRVGNLTFREKLNEMGVKTLRVVIGQDIVPKLPGLFVNSIVNKLSLVTGK 469
           VH+SVVSFGAPRVGNL FREKLNEMGVK LRVVIGQDIVPKLPGLFVNSIVNKLS VTGK
Sbjct: 361 VHVSVVSFGAPRVGNLAFREKLNEMGVKILRVVIGQDIVPKLPGLFVNSIVNKLSAVTGK 420

Query: 470 LNWVYRHVGKELRMDMYMSPYLKRESDMTGSHNLEIYLHLVDGFVSRKGKFRWNSRRDVA 529
           LNWVYRHVGKELRM+M+MSPYLK++SDM+GSHNLEIYLHLVDGFV++KGKFRWNSRRDVA
Sbjct: 421 LNWVYRHVGKELRMNMFMSPYLKKDSDMSGSHNLEIYLHLVDGFVTKKGKFRWNSRRDVA 480

Query: 530 LVNKGSDMLVEELRIPQFWYQLPHKGLIKNRFGRWVKPGRNPEDIPSPFSQPSHV 581
           LVNKGSDMLVEELRIP+FWYQLPHKGLIKNRFGRWVKPGRN EDIPSPFSQPS V
Sbjct: 481 LVNKGSDMLVEELRIPEFWYQLPHKGLIKNRFGRWVKPGRNVEDIPSPFSQPSQV 534

BLAST of Bhi08G001649 vs. NCBI nr
Match: XP_022927629.1 (phospholipase A1-Igamma1, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 963.8 bits (2490), Expect = 2.7e-277
Identity = 461/531 (86.82%), Postives = 502/531 (94.54%), Query Frame = 0

Query: 50  MEAKMATPRGVAMKRVTMLRNGTKKTKWYWKLKFGIRLKAIRSAISSSLHNQRRRLTCST 109
           ME ++ TPRGV +KRVTMLRNGTKKTKWYWKLK+GIRL+AI++A+SSSLHNQRRRLTC+T
Sbjct: 1   MEGRIVTPRGVPLKRVTMLRNGTKKTKWYWKLKWGIRLRAIKNALSSSLHNQRRRLTCAT 60

Query: 110 AIPTNTPVNGIVSPLVVHQSRSSSKKNELRLAKPLASLLRMPLRAADFIDFGNHMTPTLS 169
           A    TPVNG VSPLV+  S+S+SKKN+LRLAKPLASLLRMP RAADF+D+GNHMTPT S
Sbjct: 61  ATGQLTPVNGAVSPLVIQPSKSTSKKNDLRLAKPLASLLRMPQRAADFVDYGNHMTPTFS 120

Query: 170 PRENISAVWRDLHGASDWDGLLDPLHPFLRREIVKYGEFAQATYDAFDFDPLSEFCGSCR 229
           PR+ I+ VWRDLHG S+W+GLLDPLHPFLRRE+VKYGEFAQATYDAFDFDPLSEF GSCR
Sbjct: 121 PRDKIADVWRDLHGGSNWEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFRGSCR 180

Query: 230 YNRHKLFNELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSTDE 289
           YNRHKL +ELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVS DE
Sbjct: 181 YNRHKLLHELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSNDE 240

Query: 290 ESDRIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRSDKKVKVQRGFLTIYKSKDEESKF 349
           ES+RIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRSD+K+KVQRGFLTIYKSKDEESKF
Sbjct: 241 ESERIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRSDRKIKVQRGFLTIYKSKDEESKF 300

Query: 350 NKTSASEQVMEELNRLINFFKEKGDREISLTITGHSLGGALSLLTAYEAGVLFPTGVHIS 409
           NKTSASEQVMEEL+RLI+FF +KGDREISLTI GHSLGGALSLLTAYEAG  FP  +H+S
Sbjct: 301 NKTSASEQVMEELHRLIDFFTQKGDREISLTIVGHSLGGALSLLTAYEAGASFP-DIHVS 360

Query: 410 VVSFGAPRVGNLTFREKLNEMGVKTLRVVIGQDIVPKLPGLFVNSIVNKLSLVTGKLNWV 469
           V+SFGAPRVGNL FREKLNEMGVKTLRVVI QDIVPKLPGLFVNSIVNKLS VTGKLNW+
Sbjct: 361 VISFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVNKLSAVTGKLNWL 420

Query: 470 YRHVGKELRMDMYMSPYLKRESDMTGSHNLEIYLHLVDGFVSRKGKFRWNSRRDVALVNK 529
           YRHVG +L+MDMYMSPYLK+ESDM+GSHNLEIYLHLVDGFVSR+GKFRWNSRRDVALVNK
Sbjct: 421 YRHVGTQLKMDMYMSPYLKKESDMSGSHNLEIYLHLVDGFVSRRGKFRWNSRRDVALVNK 480

Query: 530 GSDMLVEELRIPQFWYQLPHKGLIKNRFGRWVKPGRNPEDIPSPFSQPSHV 581
           GSDMLVEELRIP+FWYQLPHKGL+KNRFGRWVKPGRN EDIPSPFS+PS++
Sbjct: 481 GSDMLVEELRIPEFWYQLPHKGLVKNRFGRWVKPGRNAEDIPSPFSRPSNI 530

BLAST of Bhi08G001649 vs. NCBI nr
Match: XP_023001410.1 (phospholipase A1-Igamma1, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 963.8 bits (2490), Expect = 2.7e-277
Identity = 462/531 (87.01%), Postives = 501/531 (94.35%), Query Frame = 0

Query: 50  MEAKMATPRGVAMKRVTMLRNGTKKTKWYWKLKFGIRLKAIRSAISSSLHNQRRRLTCST 109
           ME ++ TPRGV +KRVTMLRNGTKKTKWYWKLK+GIRL+AI++A+SSSLHNQRRRLTCST
Sbjct: 1   MEGRIVTPRGVPLKRVTMLRNGTKKTKWYWKLKWGIRLRAIKNALSSSLHNQRRRLTCST 60

Query: 110 AIPTNTPVNGIVSPLVVHQSRSSSKKNELRLAKPLASLLRMPLRAADFIDFGNHMTPTLS 169
           A    TPVNG  SPLV+  S+S++KKN+LRLAKPLASLLRMP RAADF+D+GNHMTPT S
Sbjct: 61  ATGQLTPVNGAASPLVIQPSKSTAKKNDLRLAKPLASLLRMPQRAADFVDYGNHMTPTFS 120

Query: 170 PRENISAVWRDLHGASDWDGLLDPLHPFLRREIVKYGEFAQATYDAFDFDPLSEFCGSCR 229
           PR+ I+ VWRDLHGAS+W+GLLDPLHPFLRRE+VKYGEFAQATYDAFDFDPLSE+ GSCR
Sbjct: 121 PRDKIADVWRDLHGASNWEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEYRGSCR 180

Query: 230 YNRHKLFNELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSTDE 289
           YNRHKL +ELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVS DE
Sbjct: 181 YNRHKLLHELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSDDE 240

Query: 290 ESDRIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRSDKKVKVQRGFLTIYKSKDEESKF 349
           ES+RIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRSDKK+KVQRGFLTIYKSKDEESKF
Sbjct: 241 ESERIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRSDKKIKVQRGFLTIYKSKDEESKF 300

Query: 350 NKTSASEQVMEELNRLINFFKEKGDREISLTITGHSLGGALSLLTAYEAGVLFPTGVHIS 409
           NKTSASEQVMEEL+RLI FF +KGDREISLTI GHSLGGALSLLTAYEAGV FP  +H+S
Sbjct: 301 NKTSASEQVMEELHRLIGFFTQKGDREISLTIVGHSLGGALSLLTAYEAGVSFP-DIHVS 360

Query: 410 VVSFGAPRVGNLTFREKLNEMGVKTLRVVIGQDIVPKLPGLFVNSIVNKLSLVTGKLNWV 469
           V+SFGAPRVGNL FREKLNEMGVKTLRVVI QDIVPKLPGLFVNSIVNKLS VTGKLNW+
Sbjct: 361 VISFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVNKLSAVTGKLNWL 420

Query: 470 YRHVGKELRMDMYMSPYLKRESDMTGSHNLEIYLHLVDGFVSRKGKFRWNSRRDVALVNK 529
           YRHVG +L+MDMYMSPYLKRESDM+GSHNLEIYLHLVDGFVSR+GKFRWNSRRDVALVNK
Sbjct: 421 YRHVGTQLKMDMYMSPYLKRESDMSGSHNLEIYLHLVDGFVSRRGKFRWNSRRDVALVNK 480

Query: 530 GSDMLVEELRIPQFWYQLPHKGLIKNRFGRWVKPGRNPEDIPSPFSQPSHV 581
           GSDMLVEELRIP+FWYQLPHKGL+KNRFGRWVKPGRN EDIPSPF +PS++
Sbjct: 481 GSDMLVEELRIPEFWYQLPHKGLVKNRFGRWVKPGRNAEDIPSPFPRPSNI 530

BLAST of Bhi08G001649 vs. NCBI nr
Match: XP_023519232.1 (phospholipase A1-Igamma1, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 961.4 bits (2484), Expect = 1.3e-276
Identity = 460/531 (86.63%), Postives = 501/531 (94.35%), Query Frame = 0

Query: 50  MEAKMATPRGVAMKRVTMLRNGTKKTKWYWKLKFGIRLKAIRSAISSSLHNQRRRLTCST 109
           ME ++ TPRGV +KRVTMLRNGTKKTKWYWKLK+GIRL+AI++A+SSSLHNQRRRLTC+T
Sbjct: 1   MEGRIVTPRGVPLKRVTMLRNGTKKTKWYWKLKWGIRLRAIKNALSSSLHNQRRRLTCAT 60

Query: 110 AIPTNTPVNGIVSPLVVHQSRSSSKKNELRLAKPLASLLRMPLRAADFIDFGNHMTPTLS 169
           A    TPVNG  SPLV+  S+S+SKKN+LRLAKPLASLLRMP RAADF+D+GNHMTPT S
Sbjct: 61  ATGQLTPVNGAASPLVIQPSKSTSKKNDLRLAKPLASLLRMPQRAADFVDYGNHMTPTFS 120

Query: 170 PRENISAVWRDLHGASDWDGLLDPLHPFLRREIVKYGEFAQATYDAFDFDPLSEFCGSCR 229
           PR+ I+ VWRDLHG S+W+GLLDPLHPFLRRE+VKYGEFAQATYDAFDFDPLSEF GSCR
Sbjct: 121 PRDKIADVWRDLHGGSNWEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFRGSCR 180

Query: 230 YNRHKLFNELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSTDE 289
           YNRHKL +ELGLAQNGYKVT+YIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVS DE
Sbjct: 181 YNRHKLLHELGLAQNGYKVTRYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSNDE 240

Query: 290 ESDRIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRSDKKVKVQRGFLTIYKSKDEESKF 349
           ES+RIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRSD+K+KVQRGFLTIYKSKDEESKF
Sbjct: 241 ESERIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRSDRKIKVQRGFLTIYKSKDEESKF 300

Query: 350 NKTSASEQVMEELNRLINFFKEKGDREISLTITGHSLGGALSLLTAYEAGVLFPTGVHIS 409
           NKTSASEQVMEEL+RLI+FF +KGDREISLTI GHSLGGALSLLTAYEAG  FP  +H+S
Sbjct: 301 NKTSASEQVMEELHRLIDFFTQKGDREISLTIVGHSLGGALSLLTAYEAGASFP-DIHVS 360

Query: 410 VVSFGAPRVGNLTFREKLNEMGVKTLRVVIGQDIVPKLPGLFVNSIVNKLSLVTGKLNWV 469
           V+SFGAPRVGNL FREKLNEMGVKTLRVVI QDIVPKLPGLFVNSIVNKLS VTGKLNW+
Sbjct: 361 VMSFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVNKLSAVTGKLNWL 420

Query: 470 YRHVGKELRMDMYMSPYLKRESDMTGSHNLEIYLHLVDGFVSRKGKFRWNSRRDVALVNK 529
           YRHVG +L+MDMYMSPYLKRESDM+GSHNLEIYLHLVDGFVSR+GKFRWNSRRDVALVNK
Sbjct: 421 YRHVGTQLKMDMYMSPYLKRESDMSGSHNLEIYLHLVDGFVSRRGKFRWNSRRDVALVNK 480

Query: 530 GSDMLVEELRIPQFWYQLPHKGLIKNRFGRWVKPGRNPEDIPSPFSQPSHV 581
           GSDMLVEELRIP+FWYQLPHKGL+KNRFGRWVKPGRN EDIPSPFS+PS++
Sbjct: 481 GSDMLVEELRIPEFWYQLPHKGLVKNRFGRWVKPGRNAEDIPSPFSRPSNI 530

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
sp|Q3EBR6|PLA16_ARATH1.3e-10847.80Phospholipase A1-Igamma2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At2g3... [more]
sp|Q941F1|PLA15_ARATH5.3e-10746.02Phospholipase A1-Igamma1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At1g0... [more]
sp|Q9C8J6|PLA17_ARATH9.6e-10145.39Phospholipase A1-Igamma3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At1g5... [more]
sp|O23522|PLA14_ARATH4.4e-6940.43Phospholipase A1-Ibeta2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At4g16... [more]
sp|Q9LNC2|PLA18_ARATH4.4e-6937.90Phospholipase A1-IIalpha OS=Arabidopsis thaliana OX=3702 GN=At1g06250 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AT1G30370.11.1e-16856.57alpha/beta-Hydrolases superfamily protein[more]
AT2G30550.26.9e-11047.80alpha/beta-Hydrolases superfamily protein[more]
AT1G06800.12.9e-10846.02alpha/beta-Hydrolases superfamily protein[more]
AT1G51440.15.3e-10245.39alpha/beta-Hydrolases superfamily protein[more]
AT1G06250.12.4e-7037.90alpha/beta-Hydrolases superfamily protein[more]
Match NameE-valueIdentityDescription
tr|A0A1S3BBX3|A0A1S3BBX3_CUCME2.9e-28890.49phospholipase A1-Igamma1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103487991 ... [more]
tr|A0A0A0M111|A0A0A0M111_CUCSA6.1e-28689.91Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G537320 PE=4 SV=1[more]
tr|A0A2P4HZ01|A0A2P4HZ01_QUESU9.1e-19764.86Phospholipase a1-igamma2, chloroplastic OS=Quercus suber OX=58331 GN=CFP56_46530... [more]
tr|A0A2N9FVF1|A0A2N9FVF1_FAGSY3.9e-19263.25Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS18783 PE=4 SV=1[more]
tr|A0A2C9U5J1|A0A2C9U5J1_MANES7.7e-18861.97Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_17G074500 PE=4 SV=... [more]
Match NameE-valueIdentityDescription
XP_008444745.14.4e-28890.49PREDICTED: phospholipase A1-Igamma1, chloroplastic [Cucumis melo][more]
XP_004135535.19.3e-28689.91PREDICTED: phospholipase A1-Igamma1, chloroplastic [Cucumis sativus] >KGN65881.1... [more]
XP_022927629.12.7e-27786.82phospholipase A1-Igamma1, chloroplastic-like [Cucurbita moschata][more]
XP_023001410.12.7e-27787.01phospholipase A1-Igamma1, chloroplastic-like [Cucurbita maxima][more]
XP_023519232.11.3e-27686.63phospholipase A1-Igamma1, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0006629lipid metabolic process
Vocabulary: INTERPRO
TermDefinition
IPR029058AB_hydrolase
IPR002921Fungal_lipase-like
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006629 lipid metabolic process
biological_process GO:0010214 seed coat development
cellular_component GO:0005575 cellular_component
cellular_component GO:0005739 mitochondrion
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0008970 phosphatidylcholine 1-acylhydrolase activity
molecular_function GO:0003674 molecular_function

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi08M001649Bhi08M001649mRNA


Analysis Name: InterPro Annotations of wax gourd
Date Performed: 2019-11-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002921Fungal lipase-like domainPFAMPF01764Lipase_3coord: 301..450
e-value: 1.4E-33
score: 115.7
NoneNo IPR availableGENE3DG3DSA:3.40.50.12520coord: 174..572
e-value: 4.0E-141
score: 472.6
NoneNo IPR availablePANTHERPTHR31403:SF13DAD1-LIKE ACYLHYDROLASEcoord: 67..570
NoneNo IPR availablePANTHERPTHR31403FAMILY NOT NAMEDcoord: 67..570
NoneNo IPR availableCDDcd00519Lipase_3coord: 231..507
e-value: 1.25083E-46
score: 163.416
IPR029058Alpha/Beta hydrolase foldSUPERFAMILYSSF53474alpha/beta-Hydrolasescoord: 241..507