Cp4.1LG20g03400 (gene) Cucurbita pepo (Zucchini) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cp4.1LG20g03400
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (Zucchini))
Description	Pectate lyase
Location	Cp4.1LG20 : 1966050 .. 1967671 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

CCATGGCTTCCTCATCATCCCACCTTCTTCTAACTCTTCTCATCATTATCTCACTCATACAATCCTCCCATCAAGATTACTACACCCCAACCAAGAACAACATGAACATAATCGACTCTTGTTGGCGTTCATCCTCCAATTGGGCCAAAAACCGCCAAGCCTTAGCCAACTGCGCGGTGGGCTACGGCAAGGACGCAATTGGAGGCAAATTTGGCACCATTTACGTAGTCACCGACCCATCTGACAACCCTTCAAACCCCAAATATGGAACGTTGCGTTATGGTGTGATTCAAAACAAGCCACTTTGGGTTGTGTTTGCTAAGAGCATGGTTATTGTGTTGAAGAATGAGCTGATGATAAATAGTTTCAAGACCATTGATGGACGTGGTGCTAATGTGGAGATTGCTTATGGGCCTTGCATTACGGTGCAAGGGGTTAGCCATGTTATTATACATGGAATTAGTATCCATGATTGTAAGCCTGCCAAAGCTGGGCTTGTTAGAGACACTGTCACACATGTTGGCAAACGTAGAGGCTCTGATGGTGATGCCATTTCTATATTTGCTTCCTCCCATGTTTGGATTGATCATTGTTACTTAGCTCAATCCACTGATGGTCTTATTGATATAATTCATGCTTCCACCGCTGTTACTGTCTCAAATAACTACTTCTCCCAACATGACAAGGTAAGAATGTTTTTTTAAGATTCTTTCTGTCAGATCCCATGTCAGTGTAAAAATTCTTTATAAGAGTGTAAAAATCTCTCCTTATTAACACATTCTAAAATTTGGGTTTCGTTTTTGATAGGTGATGCTGCTTGGACATAACGATGGGTTCACCGATGATAAAATTATGAGGGTCACCATTGTCTTCAACCGCTTCGGAGCTGGCCTTATCGAGAGGATGCCGAGGTAAAAAAAAAAAAAAACGCTATAAAATGTTCTCATACCAACATATGCAAGTCATACATTCATACTCAGAATTTACGAAAAAGTACTATAAAGATCTGCAATAACATTTTTTTTTGTTAGCTATCATATCCATTATTTCTTGTAGTGTAAATTAAAGACTAACCCTTTACTTATTATCGAAATTTCTTATAGTTTAAGACGAAATTTGTGATTTAACGAGTGTTATATGAATATCCCTAATGTTTTGTTATCTGGGTATTAATAATGATATAGGGTTAGGTTCGGTTACGCCCACGTAGCCAACAATCGATACGACGAGTGGAAAATGTACGCGATAGGTGGGAGTGCAAACCCCACAATCTTCAGTGAAGGGAACTACTTCGTAGCTCCCAAGAACTCAAATGCCAAACAGGTAATACATAGCAACAACCACTTCACTTCAAGAATCAAAAATTTAAATTTGGATGTAAAATAAAGCATTTGAACAACGAACAGGTAACAAAGAGAGAGGTCAACAAAGGGTGGAATAACTGGAAATGGAGATCCTCAAGAGACGTGTTCTTGAACGGCGCTTACTTTGTGCAATCTGGGTGGGGAAGTTGCAGCCCATTCTACACAAGAGCTCAGTCCTTTCCAGTGGCTCCAGGCTCCAAGGTTCCAGCTTTGACTGCAAATTCTGGGCCACTTCGTTGCTCCATTGGCAAACGTTGC

mRNA sequence

CCATGGCTTCCTCATCATCCCACCTTCTTCTAACTCTTCTCATCATTATCTCACTCATACAATCCTCCCATCAAGATTACTACACCCCAACCAAGAACAACATGAACATAATCGACTCTTGTTGGCGTTCATCCTCCAATTGGGCCAAAAACCGCCAAGCCTTAGCCAACTGCGCGGTGGGCTACGGCAAGGACGCAATTGGAGGCAAATTTGGCACCATTTACGTAGTCACCGACCCATCTGACAACCCTTCAAACCCCAAATATGGAACGTTGCGTTATGGTGTGATTCAAAACAAGCCACTTTGGGTTGTGTTTGCTAAGAGCATGGTTATTGTGTTGAAGAATGAGCTGATGATAAATAGTTTCAAGACCATTGATGGACGTGGTGCTAATGTGGAGATTGCTTATGGGCCTTGCATTACGGTGCAAGGGGTTAGCCATGTTATTATACATGGAATTAGTATCCATGATTGTAAGCCTGCCAAAGCTGGGCTTGTTAGAGACACTGTCACACATGTTGGCAAACGTAGAGGCTCTGATGGTGATGCCATTTCTATATTTGCTTCCTCCCATGTTTGGATTGATCATTGTTACTTAGCTCAATCCACTGATGCCAACAATCGATACGACGAGTGGAAAATGTACGCGATAGGTGGGAGTGCAAACCCCACAATCTTCAGTGAAGGGAACTACTTCGTAGCTCCCAAGAACTCAAATGCCAAACAGGTAACAAAGAGAGAGGTCAACAAAGGGTGGAATAACTGGAAATGGAGATCCTCAAGAGACGTGTTCTTGAACGGCGCTTACTTTGTGCAATCTGGGTGGGGAAGTTGCAGCCCATTCTACACAAGAGCTCAGTCCTTTCCAGTGGCTCCAGGCTCCAAGGTTCCAGCTTTGACTGCAAATTCTGGGCCACTTCGTTGCTCCATTGGCAAACGTTGC

Coding sequence (CDS)

ATGGCTTCCTCATCATCCCACCTTCTTCTAACTCTTCTCATCATTATCTCACTCATACAATCCTCCCATCAAGATTACTACACCCCAACCAAGAACAACATGAACATAATCGACTCTTGTTGGCGTTCATCCTCCAATTGGGCCAAAAACCGCCAAGCCTTAGCCAACTGCGCGGTGGGCTACGGCAAGGACGCAATTGGAGGCAAATTTGGCACCATTTACGTAGTCACCGACCCATCTGACAACCCTTCAAACCCCAAATATGGAACGTTGCGTTATGGTGTGATTCAAAACAAGCCACTTTGGGTTGTGTTTGCTAAGAGCATGGTTATTGTGTTGAAGAATGAGCTGATGATAAATAGTTTCAAGACCATTGATGGACGTGGTGCTAATGTGGAGATTGCTTATGGGCCTTGCATTACGGTGCAAGGGGTTAGCCATGTTATTATACATGGAATTAGTATCCATGATTGTAAGCCTGCCAAAGCTGGGCTTGTTAGAGACACTGTCACACATGTTGGCAAACGTAGAGGCTCTGATGGTGATGCCATTTCTATATTTGCTTCCTCCCATGTTTGGATTGATCATTGTTACTTAGCTCAATCCACTGATGCCAACAATCGATACGACGAGTGGAAAATGTACGCGATAGGTGGGAGTGCAAACCCCACAATCTTCAGTGAAGGGAACTACTTCGTAGCTCCCAAGAACTCAAATGCCAAACAGGTAACAAAGAGAGAGGTCAACAAAGGGTGGAATAACTGGAAATGGAGATCCTCAAGAGACGTGTTCTTGAACGGCGCTTACTTTGTGCAATCTGGGTGGGGAAGTTGCAGCCCATTCTACACAAGAGCTCAGTCCTTTCCAGTGGCTCCAGGCTCCAAGGTTCCAGCTTTGACTGCAAATTCTGGGCCACTTCGTTGCTCCATTGGCAAACGTTGC

Protein sequence

MASSSSHLLLTLLIIISLIQSSHQDYYTPTKNNMNIIDSCWRSSSNWAKNRQALANCAVGYGKDAIGGKFGTIYVVTDPSDNPSNPKYGTLRYGVIQNKPLWVVFAKSMVIVLKNELMINSFKTIDGRGANVEIAYGPCITVQGVSHVIIHGISIHDCKPAKAGLVRDTVTHVGKRRGSDGDAISIFASSHVWIDHCYLAQSTDANNRYDEWKMYAIGGSANPTIFSEGNYFVAPKNSNAKQVTKREVNKGWNNWKWRSSRDVFLNGAYFVQSGWGSCSPFYTRAQSFPVAPGSKVPALTANSGPLRCSIGKRC

Homology

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BLAST of Cp4.1LG20g03400 vs. Swiss-Prot
Match: PLY16_ARATH (Probable pectate lyase 16 OS=Arabidopsis thaliana GN=At4g22080 PE=2 SV=1)

HSP 1 Score: 303.1 bits (775), Expect = 3.5e-81
Identity = 177/396 (44.70%), Postives = 223/396 (56.31%), Query Frame = 1


Query: 6   SHLLLTLLIIISLIQSSHQDYY--TPT---------KNNMNIIDSCWRSSSNWAKNRQAL 65
           S LL+ L +  SL+ + +  YY  TPT         +N MN +DSCWR  S+WA NR+ L
Sbjct: 7   SCLLVVLFLCHSLVHAENNGYYGYTPTVANYLPEKPQNIMNPVDSCWRLKSDWAANRKDL 66

Query: 66  ANCAVGYGKDAIGGKFGTIYVVTDPSDNPSNPKYGTLRYGVIQNKPLWVVFAKSMVIVLK 125
           A+C VG+G   +GGK G +YVVT+P DN  NP+ G+LRYGVIQ KPLW+ FAK MVI L+
Sbjct: 67  ADCVVGFGSSTLGGKKGNLYVVTNPYDNAQNPQPGSLRYGVIQAKPLWITFAKDMVITLE 126

Query: 126 NELMINSFKTIDGRGANVEIAYGPCITVQGVSHVIIHGISIHDCKPAKAGLVRDTVTHVG 185
           NELM+NS+KTIDGRGA VEIAYGPCIT+Q V++VI+HGISIHDCKP K G+VR + THVG
Sbjct: 127 NELMVNSYKTIDGRGAKVEIAYGPCITIQDVTNVIVHGISIHDCKPGKYGMVRSSPTHVG 186

Query: 186 KRRGSDGDAISIFASSHVWIDHCYLAQSTDANNRYDEWKMYAIGGSANPTIFSEGNYF-- 245
            R+GSDGDAI+IF SS++WIDHCYLA  TD         +  I  S   TI    NYF  
Sbjct: 187 HRKGSDGDAIAIFGSSNIWIDHCYLASCTDG-------LIDVIHASTGITI--SNNYFTQ 246

Query: 246 -----VAPKNSNAKQVTKREVNKGWNNW------------------------KWR----- 305
                +   N +  Q  K +V   +N++                        KW      
Sbjct: 247 HDKVMLLGHNDDFVQDVKMKVTVAFNHFGPGLVERMPRVRRGYAHVANNRYDKWIMYAIG 306

Query: 306 ----------------------------------------SSRDVFLNGAYFVQSGWGSC 315
                                                   +S+DVF NGAYFV SG+GS 
Sbjct: 307 GSADPTIFSEGNYFIASDKSNSKEVTKREVKGGWNNWRWRTSKDVFKNGAYFVPSGYGSI 366

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BLAST of Cp4.1LG20g03400 vs. Swiss-Prot
Match: PLY2_ARATH (Putative pectate lyase 2 OS=Arabidopsis thaliana GN=At1g11920 PE=3 SV=2)

HSP 1 Score: 302.4 bits (773), Expect = 5.9e-81
Identity = 166/381 (43.57%), Postives = 228/381 (59.84%), Query Frame = 1


Query: 8   LLLTLLIII------SLIQSSHQDYYTPTKNNMNIIDSCWRSSSNWAKNRQALANCAVGY 67
           L LT++ ++      S++++++ + YT  K   N IDSCWR +  WA NR+ALA+CAVG+
Sbjct: 4   LFLTIISLLFAAFSSSVVEAAYSNGYTIPKLLPNPIDSCWRRNPYWASNRRALADCAVGF 63

Query: 68  GKDAIGGKFGTIYVVTDPSDNPSNPKYGTLRYGVIQNKPLWVVFAKSMVIVLKNELMINS 127
           GK A+GGK+G+IYVVT+PSD+P NP+ GTLRY VIQ+KPLW+ FA+ MVIVL+NEL++NS
Sbjct: 64  GKSAVGGKYGSIYVVTNPSDDPENPRPGTLRYAVIQSKPLWITFARDMVIVLRNELIMNS 123

Query: 128 FKTIDGRGANVEIAYGPCITVQGVSHVIIHGISIHDCKPAKAGLVRDTVTHVGKRRGSDG 187
           +KTIDGRGA VEIAYGPCIT+Q VSHVIIHGISIHDCKP K+G VR + THVG R+GSDG
Sbjct: 124 YKTIDGRGAKVEIAYGPCITIQHVSHVIIHGISIHDCKPGKSGRVRSSPTHVGSRKGSDG 183

Query: 188 DAISIFASSHVWIDHCYLAQSTD--------------ANNRYDEW-KMYAIGGSANPT-- 247
           DAI+IF SSH+WIDHC+ ++  D              +NN + +  K+  +G + N    
Sbjct: 184 DAIAIFDSSHIWIDHCFFSRCQDGLIDVLHASTAVTISNNYFTQHDKVMLLGHNDNNVED 243

Query: 248 ----IFSEGNYF---VAPKNSNAKQVTKREVNKGWNNWKW-----RSSRDVFLNG----- 307
               +    N+F   +  +    ++      N  +  W+       +   +F  G     
Sbjct: 244 KIMRVTIAFNHFGPGLIERMPRVRRGYAHVANNRYEKWQMYAIGGSADPTIFSEGNYFVA 303

Query: 308 ----------------------------------AYFVQSGWGSCSPFYTRAQSFPVAPG 315
                                             AYFV SG+G+ +P Y RA+ FPV+ G
Sbjct: 304 SDDPSKKQVTKRIDSGYDWKRWKWRTSKDVFKNGAYFVPSGYGTVTPLYGRAERFPVSHG 363

[Close]

BLAST of Cp4.1LG20g03400 vs. Swiss-Prot
Match: PLY17_ARATH (Putative pectate lyase 17 OS=Arabidopsis thaliana GN=At4g22090 PE=3 SV=1)

HSP 1 Score: 291.2 bits (744), Expect = 1.4e-77
Identity = 169/387 (43.67%), Postives = 220/387 (56.85%), Query Frame = 1


Query: 6   SHLLLTLLIIISLIQSSHQDYY--TPT---------KNNMNIIDSCWRSSSNWAKNRQAL 65
           S LL+ L +  SL+ +++  YY  +P          +N MN +DSCWR  S+W  NR+ L
Sbjct: 7   SCLLVALFLCQSLVHAAYNGYYGYSPAAAPYPAEEPQNIMNPVDSCWRLKSDWDVNREDL 66

Query: 66  ANCAVGYGKDAIGGKFGTIYVVTDPSDNPSNPKYGTLRYGVIQNKPLWVVFAKSMVIVLK 125
           A+CAVG+G   +GGK G IYVVT+P DN  NP  G+LRYGVIQ KPLW+ FAK MVI L 
Sbjct: 67  ADCAVGFGSSTLGGKKGNIYVVTNPYDNAQNPHPGSLRYGVIQAKPLWITFAKDMVITLA 126

Query: 126 NELMINSFKTIDGRGANVEIAYGPCITVQGVSHVIIHGISIHDCKPAKAGLVRDTVTHVG 185
           NELM+NS+KTIDGRGA VEIAYGPCIT+Q V++VI+HGISIHDCKP K+G VR + THVG
Sbjct: 127 NELMVNSYKTIDGRGAKVEIAYGPCITIQDVTNVIVHGISIHDCKPGKSGKVRSSPTHVG 186

Query: 186 KRRGSDGDAISIFASSHVWIDHCYLAQSTD--------------ANNRYDEW-KMYAIGG 245
            R+GSDGDAI+IF SS+VWIDHCYLA  TD              +NN + +  K+  +G 
Sbjct: 187 HRKGSDGDAITIFGSSNVWIDHCYLASCTDGLIDVIHASTAITISNNYFTQHDKVMLLGH 246

Query: 246 SAN---------PTIFSEGNYFVAPKNSNAKQVTKREVNKGWNNW--------------- 305
           + N            F+     +  +    ++      N  ++ W               
Sbjct: 247 NDNFVKDVKMKVTVAFNHFGPGLVERMPRVRRGYAHVANNRYDKWIMYAIGGSADPTIFS 306

Query: 306 -------------KWRSSRD---------------VFLNGAYFVQSGWGSCSPFYTRAQS 315
                        K  + R+               VF NGA+FV SG+GS    Y+ AQ 
Sbjct: 307 EGNYFIASDKSYSKEVTKREVKGGWNNWRWRTSNDVFKNGAFFVPSGYGSIPLPYSSAQR 366

[Close]

BLAST of Cp4.1LG20g03400 vs. Swiss-Prot
Match: PLY4_ARATH (Probable pectate lyase 4 OS=Arabidopsis thaliana GN=At1g30350 PE=2 SV=1)

HSP 1 Score: 229.2 bits (583), Expect = 6.3e-59
Identity = 112/196 (57.14%), Postives = 137/196 (69.90%), Query Frame = 1


Query: 9   LLTLLIIISLIQSSHQDYYTPTKNNMNIIDSCWRSSSNWAKNRQALANCAVGYGKDAIGG 68
           LL       L++++H    T  + ++N ID+CWR +  WA NRQALA+CAVGYGK AIGG
Sbjct: 10  LLLAAFASPLLETAHSYNVTAPRVSLNPIDACWRRNPKWATNRQALAHCAVGYGKAAIGG 69

Query: 69  KFGTIYVVTDPSDNPSNPKYGTLRYGVIQNKPLWVVFAKSMVIVLKNELMINSFKTIDGR 128
           K G IYVVT+PSDNP+ P  GTLRY V Q KPLW+ FA+ MVIVLK++LMINS+KTIDGR
Sbjct: 70  KNGPIYVVTNPSDNPTRPSPGTLRYAVSQPKPLWITFARDMVIVLKSQLMINSYKTIDGR 129

Query: 129 GANVEIAYGPCITVQGVSHVIIHGISIHDCKPAKAGLVRDTVTHVGKRRGSDGDAISIFA 188
           GA VEIA GPC+ ++ V HVIIHGISIHDCK                  G DGD I +F 
Sbjct: 130 GAKVEIANGPCLRIRQVKHVIIHGISIHDCK--------------ADPNGMDGDGIRVFQ 189

Query: 189 SSHVWIDHCYLAQSTD 205
           S+HVWIDHC+L++  D
Sbjct: 190 STHVWIDHCFLSRCHD 191

[Close]

BLAST of Cp4.1LG20g03400 vs. Swiss-Prot
Match: PLY5_ARATH (Probable pectate lyase 5 OS=Arabidopsis thaliana GN=At1g67750 PE=2 SV=2)

HSP 1 Score: 228.8 bits (582), Expect = 8.3e-59
Identity = 139/350 (39.71%), Postives = 174/350 (49.71%), Query Frame = 1


Query: 35  NIIDSCWRSSSNWAKNRQALANCAVGYGKDAIGGKFGTIYVVTDPSDNPS-NPKYGTLRY 94
           N ID CWR    W KNRQ LA+CA+G+GK AIGG+ G IYVVTD SD    NPK GTLR+
Sbjct: 60  NPIDDCWRCDPKWEKNRQRLADCAIGFGKHAIGGRDGKIYVVTDSSDKDVVNPKPGTLRH 119

Query: 95  GVIQNKPLWVVFAKSMVIVLKNELMINSFKTIDGRGANVEIAYGPCITVQ---------- 154
            VIQ++PLW++FA+ MVI LK EL++NSFKTIDGRGA+V IA G CITVQ          
Sbjct: 120 AVIQDEPLWIIFARDMVIKLKEELIMNSFKTIDGRGASVHIAGGACITVQYVTNIIIHGV 179

Query: 155 ----------------------------------GVSHVIIHGISIHDCKPAKAGLVR-D 214
                                             G SHV +   S+ +C       +   
Sbjct: 180 NIHDCKRKGNAYVRDSPSHYGWRTASDGDAVSIFGGSHVWVDHCSLSNCADGLIDAIHGS 239

Query: 215 TVTHVGKRRGSDGDAISIFASSHVW----------------------IDHCYLAQSTDAN 274
           T   +     S  + + +   S  +                      +  C        N
Sbjct: 240 TAITISNNYLSHHNKVMLLGHSDSYTRDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVN 299

Query: 275 NRYDEWKMYAIGGSANPTIFSEGNYFVAPKNSNAKQVTKRE--VNKGWNNWKWRSSRDVF 315
           N Y  W+MYAIGGSA PTI S+GN F+AP +   K+VTK E      W  W WRS  D+F
Sbjct: 300 NDYTHWQMYAIGGSAAPTINSQGNRFLAPNDHVFKEVTKYEDAPRSKWKKWNWRSEGDLF 359

[Close]

BLAST of Cp4.1LG20g03400 vs. TrEMBL
Match: A0A0A0LU35_CUCSA (Pectate lyase OS=Cucumis sativus GN=Csa_1G059200 PE=3 SV=1)

HSP 1 Score: 371.7 bits (953), Expect = 8.8e-100
Identity = 174/204 (85.29%), Postives = 189/204 (92.65%), Query Frame = 1


Query: 1   MASSSSHLLLTLLIIISLIQSSHQDYYTPTKNNMNIIDSCWRSSSNWAKNRQALANCAVG 60
           MAS+SSHLLL LL I+SLI  S QDYYTP K NMN+IDSCWR +SNWAKNR+ALA+CAVG
Sbjct: 1   MASNSSHLLLILLFILSLINPSLQDYYTPQKKNMNVIDSCWRQTSNWAKNRKALADCAVG 60

Query: 61  YGKDAIGGKFGTIYVVTDPSDNPSNPKYGTLRYGVIQNKPLWVVFAKSMVIVLKNELMIN 120
           YGKDAIGGKFGTIY VTDPSDNPSNPKYGTLRYGVIQ+KPLW+VF K MVIVLKNELM+N
Sbjct: 61  YGKDAIGGKFGTIYTVTDPSDNPSNPKYGTLRYGVIQDKPLWIVFGKDMVIVLKNELMVN 120

Query: 121 SFKTIDGRGANVEIAYGPCITVQGVSHVIIHGISIHDCKPAKAGLVRDTVTHVGKRRGSD 180
           SFKTIDGRGA VEIAYGPCITVQGVSHVIIHGISIHDCKP KAGLVRDTV+H+GKR+GSD
Sbjct: 121 SFKTIDGRGAKVEIAYGPCITVQGVSHVIIHGISIHDCKPGKAGLVRDTVSHIGKRKGSD 180

Query: 181 GDAISIFASSHVWIDHCYLAQSTD 205
           GDAI++F SSHVWIDHCYLA+ TD
Sbjct: 181 GDAIAVFGSSHVWIDHCYLARCTD 204

[Close]

BLAST of Cp4.1LG20g03400 vs. TrEMBL
Match: A0A0S3S3R8_PHAAN (Pectate lyase OS=Vigna angularis var. angularis GN=Vigan.05G085900 PE=3 SV=1)

HSP 1 Score: 367.5 bits (942), Expect = 1.7e-98
Identity = 181/287 (63.07%), Postives = 211/287 (73.52%), Query Frame = 1


Query: 28  TPTKNNMNIIDSCWRSSSNWAKNRQALANCAVGYGKDAIGGKFGTIYVVTDPSDNPSNPK 87
           +P    MN IDSCWR  +NWA NRQ+LA+CA+G+GKDAIGGKFG +Y VTDPSD+P +PK
Sbjct: 45  SPKNVVMNTIDSCWRGKTNWASNRQSLADCAIGFGKDAIGGKFGDVYEVTDPSDDPVDPK 104

Query: 88  YGTLRYGVIQNKPLWVVFAKSMVIVLKNELMINSFKTIDGRGANVEIAYGPCITVQGVSH 147
            GTLRYG IQ +PLW+ FAK MVI LKNELM+NS+K+IDGRGA VEIA G CIT+QGVSH
Sbjct: 105 PGTLRYGAIQTEPLWITFAKDMVISLKNELMVNSYKSIDGRGAKVEIANGACITIQGVSH 164

Query: 148 VIIHGISIHDCKPAKAGLVRDTVTHVGKRRGSDGDAISIFASSHVWIDHCYLAQSTDANN 207
           VIIHGISIHDCKP   G+VR T  HVG R GSDGDAISIFASS+VWIDHC+LA+ TD   
Sbjct: 165 VIIHGISIHDCKPGDGGMVRSTPEHVGYREGSDGDAISIFASSNVWIDHCFLARCTDG-- 224

Query: 208 RYDEWKMYAIGGSANPTIFSEGNYFVAPKNSNAKQVTKREVNKGWNNWKWRSSRDVFLNG 267
                 +  I  S   TI    NYF     ++  +VTKRE ++ WNNWKWRS  D FLNG
Sbjct: 225 -----LIDVIHASTAVTI--SNNYF-----THHDKVTKRESSEKWNNWKWRSYGDEFLNG 284

Query: 268 AYFVQSGWGSCSPFYTRAQSFPVAPGSKVPALTANSGPLRCSIGKRC 315
           AYFV SG+GSCSP Y+ AQ+F     S VP LT N+GPL C + K C
Sbjct: 285 AYFVPSGYGSCSPLYSSAQTFTATQASMVPFLTLNAGPLNCVLHKPC 317

[Close]

BLAST of Cp4.1LG20g03400 vs. TrEMBL
Match: W9RY44_9ROSA (Pectate lyase OS=Morus notabilis GN=L484_014178 PE=3 SV=1)

HSP 1 Score: 357.8 bits (917), Expect = 1.3e-95
Identity = 189/352 (53.69%), Postives = 227/352 (64.49%), Query Frame = 1


Query: 31  KNNMNIIDSCWRSSSNWAKNRQALANCAVGYGKDAIGGKFGTIYVVTDPSDNPSNPKYGT 90
           KN MN+IDSCWR  S+WA NRQALANCA GYGKDA+GGK+G IY VT+PSD+P NPK GT
Sbjct: 25  KNIMNVIDSCWRGKSSWANNRQALANCAKGYGKDALGGKYGRIYTVTNPSDDPVNPKPGT 84

Query: 91  LRYGVIQNKPLWVVFAKSMVIVLKNELMINSFKTIDGRGANVEIAYGPCITVQGVSHVII 150
           LRYGVIQ KPLW+VFAK MVI LKNEL++NS+KTIDGRGANVEIAYGPCITVQGVSHVII
Sbjct: 85  LRYGVIQTKPLWIVFAKDMVIKLKNELIVNSYKTIDGRGANVEIAYGPCITVQGVSHVII 144

Query: 151 HGISIHDCKPAKAGLVRDTVTHVGKRRGSDGDAISIFASSHVWIDHCYLAQSTD------ 210
           HGISIHDCKP K G+VR ++THVG R G DGDAISIFASSHVWIDHC+L++S+D      
Sbjct: 145 HGISIHDCKPGKPGMVRSSLTHVGNRGGCDGDAISIFASSHVWIDHCFLSRSSDGLIDVI 204

Query: 211 --------ANNRYDEW-KMYAIGGS----ANPTI-----FSEGNYFVAPKNSNAKQVTKR 270
                   +NN + +  K+  +G S    A+ T+     F+     +  +    +     
Sbjct: 205 HASTAVTISNNFFSQHDKVMLLGHSDKFTADKTMKVTIAFNRFGPGLIERMPRVRFGYAH 264

Query: 271 EVNKGWNNWKWRSS--------------------------------------------RD 315
             N  ++ WK  +                                             +D
Sbjct: 265 VANNRYDQWKMYAVGGSANPTIFSEGNYFVAPTNSGAKEVTKREAVNKGWKNWKWRSSKD 324

[Close]

BLAST of Cp4.1LG20g03400 vs. TrEMBL
Match: M5X1K7_PRUPE (Pectate lyase OS=Prunus persica GN=PRUPE_ppa025559mg PE=3 SV=1)

HSP 1 Score: 347.1 bits (889), Expect = 2.3e-92
Identity = 193/385 (50.13%), Postives = 236/385 (61.30%), Query Frame = 1


Query: 1   MASSSSHLLLTLLI---IISLIQSSHQDYYTPT-KNNMNIIDSCWRSSSNWAKNRQALAN 60
           MAS S  +LL+  +   I    Q+    YYT T K  MN++DSCWRS SNWA NR+ALAN
Sbjct: 1   MASLSPLILLSCFLTYYIAPTSQAYSPGYYTTTPKTTMNVVDSCWRSKSNWAINRRALAN 60

Query: 61  CAVGYGKDAIGGKFGTIYVVTDPSDNPSNPKYGTLRYGVIQNKPLWVVFAKSMVIVLKNE 120
           CAVG+G+DA+GGK+G+ YVVT   D+P NPK G+LRYGVIQ +PLW+VFAK MVI LKNE
Sbjct: 61  CAVGFGQDAVGGKYGSTYVVTTSYDDPINPKPGSLRYGVIQTQPLWIVFAKDMVITLKNE 120

Query: 121 LMINSFKTIDGRGANVEIAYGPCITVQGVSHVIIHGISIHDCKPAKAGLVRDTVTHVGKR 180
           L++NSFKTIDGRGA VEIAYGPCITVQGVSHVIIHGISIHDCKP K G VR T THVG R
Sbjct: 121 LIMNSFKTIDGRGAKVEIAYGPCITVQGVSHVIIHGISIHDCKPGKGGNVRSTPTHVGHR 180

Query: 181 RGSDGDAISIFASSHVWIDHCYLAQSTD--------------ANNRYDEW-KMYAIGGSA 240
           RG DGDAISIFASSHVW+DHC+LA+S+D               NN + +  K+  +G + 
Sbjct: 181 RGCDGDAISIFASSHVWVDHCFLARSSDGLIDVTHASTAVTITNNYFSQHDKVMLLGHND 240

Query: 241 NPTIFSEGNYFVAPKNSNAKQVTK---------REVNKGWNNWKWR-------------- 300
           N +        +A     A  + +            N  ++ WK                
Sbjct: 241 NFSADKGMKVTIAFNRFGAGLIERMPRVRFGYAHVANNRYDEWKMYAVGGSANPTIFSEG 300

Query: 301 -----------------------------SSRDVFLNGAYFVQSGWGSCSPFYTRAQSFP 315
                                        SS+D+F NGA+FVQSG+GSC P Y++ QSF 
Sbjct: 301 NYFIAPETAYAKQVTKRESGGRWNNWKWRSSKDMFKNGAFFVQSGYGSCYPLYSKTQSFK 360

[Close]

BLAST of Cp4.1LG20g03400 vs. TrEMBL
Match: U5FUZ6_POPTR (Pectate lyase (Fragment) OS=Populus trichocarpa GN=POPTR_0011s002501g PE=3 SV=1)

HSP 1 Score: 342.8 bits (878), Expect = 4.4e-91
Identity = 181/329 (55.02%), Postives = 223/329 (67.78%), Query Frame = 1


Query: 34  MNIIDSCWRSSSNWAKNRQALANCAVGYGKDAIGGKFGTIYVVTDPSDNPSNPKYGTLRY 93
           +N IDSCWR+ SNWA NR+ALA+CAVG+G+ A+GGK+G IYVVT P+D+P NPK G LRY
Sbjct: 3   LNTIDSCWRAQSNWANNRRALADCAVGFGRGAMGGKYGAIYVVTTPNDDPVNPKPGMLRY 62

Query: 94  GVIQNKPLWVVFAKSMVIVLKNELMINSFKTIDGRGANVEIAYGPCITVQGVSHVIIHGI 153
           G IQ+KPLW+VFAK MVI L+NEL++NS+KTIDGRGA VEIAYGPCIT+QGVSHVIIHGI
Sbjct: 63  GAIQSKPLWIVFAKDMVITLRNELIMNSYKTIDGRGAKVEIAYGPCITIQGVSHVIIHGI 122

Query: 154 SIHDCKPAKAGLVRDTVTHVGKRRGSDGDAISIFASSHVWIDHCYLAQSTDA-------- 213
           SIHDCKP K+G V  T THVGKR GSDGDAI+IFASS+VWIDHCYLA+ TD         
Sbjct: 123 SIHDCKPGKSGRVISTPTHVGKRGGSDGDAIAIFASSNVWIDHCYLARCTDGLIDVMLLG 182

Query: 214 -NNRYDEWKMYAIGGSAN----------PTI-----------FSEGNYFVAPKNSN---- 273
            N+ Y   K+  +  + N          P +           + E   +    ++N    
Sbjct: 183 HNDGYTADKVMKVTIAFNRFGSGLIERMPRVRFGYAHVANNRYDEWQMYAIGGSANPTIF 242

Query: 274 -------AKQVTKREVNKGWNNWK--W-----RSSRDVFLNGAYFVQSGWGSCSPFYTRA 315
                  A+    ++V K     K  W     RSS+DVF+NGAYFVQSG+GSC+P Y++ 
Sbjct: 243 SEGNYFLARNGNSKQVTK--REAKNGWTNWKWRSSKDVFMNGAYFVQSGYGSCAPLYSKT 302

[Close]

BLAST of Cp4.1LG20g03400 vs. TAIR10
Match: AT4G22080.1 (AT4G22080.1 root hair specific 14)

HSP 1 Score: 303.1 bits (775), Expect = 1.9e-82
Identity = 177/396 (44.70%), Postives = 223/396 (56.31%), Query Frame = 1


Query: 6   SHLLLTLLIIISLIQSSHQDYY--TPT---------KNNMNIIDSCWRSSSNWAKNRQAL 65
           S LL+ L +  SL+ + +  YY  TPT         +N MN +DSCWR  S+WA NR+ L
Sbjct: 7   SCLLVVLFLCHSLVHAENNGYYGYTPTVANYLPEKPQNIMNPVDSCWRLKSDWAANRKDL 66

Query: 66  ANCAVGYGKDAIGGKFGTIYVVTDPSDNPSNPKYGTLRYGVIQNKPLWVVFAKSMVIVLK 125
           A+C VG+G   +GGK G +YVVT+P DN  NP+ G+LRYGVIQ KPLW+ FAK MVI L+
Sbjct: 67  ADCVVGFGSSTLGGKKGNLYVVTNPYDNAQNPQPGSLRYGVIQAKPLWITFAKDMVITLE 126

Query: 126 NELMINSFKTIDGRGANVEIAYGPCITVQGVSHVIIHGISIHDCKPAKAGLVRDTVTHVG 185
           NELM+NS+KTIDGRGA VEIAYGPCIT+Q V++VI+HGISIHDCKP K G+VR + THVG
Sbjct: 127 NELMVNSYKTIDGRGAKVEIAYGPCITIQDVTNVIVHGISIHDCKPGKYGMVRSSPTHVG 186

Query: 186 KRRGSDGDAISIFASSHVWIDHCYLAQSTDANNRYDEWKMYAIGGSANPTIFSEGNYF-- 245
            R+GSDGDAI+IF SS++WIDHCYLA  TD         +  I  S   TI    NYF  
Sbjct: 187 HRKGSDGDAIAIFGSSNIWIDHCYLASCTDG-------LIDVIHASTGITI--SNNYFTQ 246

Query: 246 -----VAPKNSNAKQVTKREVNKGWNNW------------------------KWR----- 305
                +   N +  Q  K +V   +N++                        KW      
Sbjct: 247 HDKVMLLGHNDDFVQDVKMKVTVAFNHFGPGLVERMPRVRRGYAHVANNRYDKWIMYAIG 306

Query: 306 ----------------------------------------SSRDVFLNGAYFVQSGWGSC 315
                                                   +S+DVF NGAYFV SG+GS 
Sbjct: 307 GSADPTIFSEGNYFIASDKSNSKEVTKREVKGGWNNWRWRTSKDVFKNGAYFVPSGYGSI 366

[Close]

BLAST of Cp4.1LG20g03400 vs. TAIR10
Match: AT1G11920.1 (AT1G11920.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 302.4 bits (773), Expect = 3.3e-82
Identity = 166/381 (43.57%), Postives = 228/381 (59.84%), Query Frame = 1


Query: 8   LLLTLLIII------SLIQSSHQDYYTPTKNNMNIIDSCWRSSSNWAKNRQALANCAVGY 67
           L LT++ ++      S++++++ + YT  K   N IDSCWR +  WA NR+ALA+CAVG+
Sbjct: 4   LFLTIISLLFAAFSSSVVEAAYSNGYTIPKLLPNPIDSCWRRNPYWASNRRALADCAVGF 63

Query: 68  GKDAIGGKFGTIYVVTDPSDNPSNPKYGTLRYGVIQNKPLWVVFAKSMVIVLKNELMINS 127
           GK A+GGK+G+IYVVT+PSD+P NP+ GTLRY VIQ+KPLW+ FA+ MVIVL+NEL++NS
Sbjct: 64  GKSAVGGKYGSIYVVTNPSDDPENPRPGTLRYAVIQSKPLWITFARDMVIVLRNELIMNS 123

Query: 128 FKTIDGRGANVEIAYGPCITVQGVSHVIIHGISIHDCKPAKAGLVRDTVTHVGKRRGSDG 187
           +KTIDGRGA VEIAYGPCIT+Q VSHVIIHGISIHDCKP K+G VR + THVG R+GSDG
Sbjct: 124 YKTIDGRGAKVEIAYGPCITIQHVSHVIIHGISIHDCKPGKSGRVRSSPTHVGSRKGSDG 183

Query: 188 DAISIFASSHVWIDHCYLAQSTD--------------ANNRYDEW-KMYAIGGSANPT-- 247
           DAI+IF SSH+WIDHC+ ++  D              +NN + +  K+  +G + N    
Sbjct: 184 DAIAIFDSSHIWIDHCFFSRCQDGLIDVLHASTAVTISNNYFTQHDKVMLLGHNDNNVED 243

Query: 248 ----IFSEGNYF---VAPKNSNAKQVTKREVNKGWNNWKW-----RSSRDVFLNG----- 307
               +    N+F   +  +    ++      N  +  W+       +   +F  G     
Sbjct: 244 KIMRVTIAFNHFGPGLIERMPRVRRGYAHVANNRYEKWQMYAIGGSADPTIFSEGNYFVA 303

Query: 308 ----------------------------------AYFVQSGWGSCSPFYTRAQSFPVAPG 315
                                             AYFV SG+G+ +P Y RA+ FPV+ G
Sbjct: 304 SDDPSKKQVTKRIDSGYDWKRWKWRTSKDVFKNGAYFVPSGYGTVTPLYGRAERFPVSHG 363

[Close]

BLAST of Cp4.1LG20g03400 vs. TAIR10
Match: AT4G22090.1 (AT4G22090.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 291.2 bits (744), Expect = 7.6e-79
Identity = 169/387 (43.67%), Postives = 220/387 (56.85%), Query Frame = 1


Query: 6   SHLLLTLLIIISLIQSSHQDYY--TPT---------KNNMNIIDSCWRSSSNWAKNRQAL 65
           S LL+ L +  SL+ +++  YY  +P          +N MN +DSCWR  S+W  NR+ L
Sbjct: 7   SCLLVALFLCQSLVHAAYNGYYGYSPAAAPYPAEEPQNIMNPVDSCWRLKSDWDVNREDL 66

Query: 66  ANCAVGYGKDAIGGKFGTIYVVTDPSDNPSNPKYGTLRYGVIQNKPLWVVFAKSMVIVLK 125
           A+CAVG+G   +GGK G IYVVT+P DN  NP  G+LRYGVIQ KPLW+ FAK MVI L 
Sbjct: 67  ADCAVGFGSSTLGGKKGNIYVVTNPYDNAQNPHPGSLRYGVIQAKPLWITFAKDMVITLA 126

Query: 126 NELMINSFKTIDGRGANVEIAYGPCITVQGVSHVIIHGISIHDCKPAKAGLVRDTVTHVG 185
           NELM+NS+KTIDGRGA VEIAYGPCIT+Q V++VI+HGISIHDCKP K+G VR + THVG
Sbjct: 127 NELMVNSYKTIDGRGAKVEIAYGPCITIQDVTNVIVHGISIHDCKPGKSGKVRSSPTHVG 186

Query: 186 KRRGSDGDAISIFASSHVWIDHCYLAQSTD--------------ANNRYDEW-KMYAIGG 245
            R+GSDGDAI+IF SS+VWIDHCYLA  TD              +NN + +  K+  +G 
Sbjct: 187 HRKGSDGDAITIFGSSNVWIDHCYLASCTDGLIDVIHASTAITISNNYFTQHDKVMLLGH 246

Query: 246 SAN---------PTIFSEGNYFVAPKNSNAKQVTKREVNKGWNNW--------------- 305
           + N            F+     +  +    ++      N  ++ W               
Sbjct: 247 NDNFVKDVKMKVTVAFNHFGPGLVERMPRVRRGYAHVANNRYDKWIMYAIGGSADPTIFS 306

Query: 306 -------------KWRSSRD---------------VFLNGAYFVQSGWGSCSPFYTRAQS 315
                        K  + R+               VF NGA+FV SG+GS    Y+ AQ 
Sbjct: 307 EGNYFIASDKSYSKEVTKREVKGGWNNWRWRTSNDVFKNGAFFVPSGYGSIPLPYSSAQR 366

[Close]

BLAST of Cp4.1LG20g03400 vs. TAIR10
Match: AT1G30350.1 (AT1G30350.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 229.2 bits (583), Expect = 3.6e-60
Identity = 112/196 (57.14%), Postives = 137/196 (69.90%), Query Frame = 1


Query: 9   LLTLLIIISLIQSSHQDYYTPTKNNMNIIDSCWRSSSNWAKNRQALANCAVGYGKDAIGG 68
           LL       L++++H    T  + ++N ID+CWR +  WA NRQALA+CAVGYGK AIGG
Sbjct: 10  LLLAAFASPLLETAHSYNVTAPRVSLNPIDACWRRNPKWATNRQALAHCAVGYGKAAIGG 69

Query: 69  KFGTIYVVTDPSDNPSNPKYGTLRYGVIQNKPLWVVFAKSMVIVLKNELMINSFKTIDGR 128
           K G IYVVT+PSDNP+ P  GTLRY V Q KPLW+ FA+ MVIVLK++LMINS+KTIDGR
Sbjct: 70  KNGPIYVVTNPSDNPTRPSPGTLRYAVSQPKPLWITFARDMVIVLKSQLMINSYKTIDGR 129

Query: 129 GANVEIAYGPCITVQGVSHVIIHGISIHDCKPAKAGLVRDTVTHVGKRRGSDGDAISIFA 188
           GA VEIA GPC+ ++ V HVIIHGISIHDCK                  G DGD I +F 
Sbjct: 130 GAKVEIANGPCLRIRQVKHVIIHGISIHDCK--------------ADPNGMDGDGIRVFQ 189

Query: 189 SSHVWIDHCYLAQSTD 205
           S+HVWIDHC+L++  D
Sbjct: 190 STHVWIDHCFLSRCHD 191

[Close]

BLAST of Cp4.1LG20g03400 vs. TAIR10
Match: AT1G67750.1 (AT1G67750.1 Pectate lyase family protein)

HSP 1 Score: 228.8 bits (582), Expect = 4.7e-60
Identity = 139/350 (39.71%), Postives = 174/350 (49.71%), Query Frame = 1


Query: 35  NIIDSCWRSSSNWAKNRQALANCAVGYGKDAIGGKFGTIYVVTDPSDNPS-NPKYGTLRY 94
           N ID CWR    W KNRQ LA+CA+G+GK AIGG+ G IYVVTD SD    NPK GTLR+
Sbjct: 60  NPIDDCWRCDPKWEKNRQRLADCAIGFGKHAIGGRDGKIYVVTDSSDKDVVNPKPGTLRH 119

Query: 95  GVIQNKPLWVVFAKSMVIVLKNELMINSFKTIDGRGANVEIAYGPCITVQ---------- 154
            VIQ++PLW++FA+ MVI LK EL++NSFKTIDGRGA+V IA G CITVQ          
Sbjct: 120 AVIQDEPLWIIFARDMVIKLKEELIMNSFKTIDGRGASVHIAGGACITVQYVTNIIIHGV 179

Query: 155 ----------------------------------GVSHVIIHGISIHDCKPAKAGLVR-D 214
                                             G SHV +   S+ +C       +   
Sbjct: 180 NIHDCKRKGNAYVRDSPSHYGWRTASDGDAVSIFGGSHVWVDHCSLSNCADGLIDAIHGS 239

Query: 215 TVTHVGKRRGSDGDAISIFASSHVW----------------------IDHCYLAQSTDAN 274
           T   +     S  + + +   S  +                      +  C        N
Sbjct: 240 TAITISNNYLSHHNKVMLLGHSDSYTRDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVN 299

Query: 275 NRYDEWKMYAIGGSANPTIFSEGNYFVAPKNSNAKQVTKRE--VNKGWNNWKWRSSRDVF 315
           N Y  W+MYAIGGSA PTI S+GN F+AP +   K+VTK E      W  W WRS  D+F
Sbjct: 300 NDYTHWQMYAIGGSAAPTINSQGNRFLAPNDHVFKEVTKYEDAPRSKWKKWNWRSEGDLF 359

[Close]

BLAST of Cp4.1LG20g03400 vs. NCBI nr
Match: gi|449470674|ref|XP_004153041.1| (PREDICTED: putative pectate lyase 2 [Cucumis sativus])

HSP 1 Score: 371.7 bits (953), Expect = 1.3e-99
Identity = 174/204 (85.29%), Postives = 189/204 (92.65%), Query Frame = 1


Query: 1   MASSSSHLLLTLLIIISLIQSSHQDYYTPTKNNMNIIDSCWRSSSNWAKNRQALANCAVG 60
           MAS+SSHLLL LL I+SLI  S QDYYTP K NMN+IDSCWR +SNWAKNR+ALA+CAVG
Sbjct: 1   MASNSSHLLLILLFILSLINPSLQDYYTPQKKNMNVIDSCWRQTSNWAKNRKALADCAVG 60

Query: 61  YGKDAIGGKFGTIYVVTDPSDNPSNPKYGTLRYGVIQNKPLWVVFAKSMVIVLKNELMIN 120
           YGKDAIGGKFGTIY VTDPSDNPSNPKYGTLRYGVIQ+KPLW+VF K MVIVLKNELM+N
Sbjct: 61  YGKDAIGGKFGTIYTVTDPSDNPSNPKYGTLRYGVIQDKPLWIVFGKDMVIVLKNELMVN 120

Query: 121 SFKTIDGRGANVEIAYGPCITVQGVSHVIIHGISIHDCKPAKAGLVRDTVTHVGKRRGSD 180
           SFKTIDGRGA VEIAYGPCITVQGVSHVIIHGISIHDCKP KAGLVRDTV+H+GKR+GSD
Sbjct: 121 SFKTIDGRGAKVEIAYGPCITVQGVSHVIIHGISIHDCKPGKAGLVRDTVSHIGKRKGSD 180

Query: 181 GDAISIFASSHVWIDHCYLAQSTD 205
           GDAI++F SSHVWIDHCYLA+ TD
Sbjct: 181 GDAIAVFGSSHVWIDHCYLARCTD 204

[Close]

BLAST of Cp4.1LG20g03400 vs. NCBI nr
Match: gi|965600306|dbj|BAT87484.1| (hypothetical protein VIGAN_05085900 [Vigna angularis var. angularis])

HSP 1 Score: 367.5 bits (942), Expect = 2.4e-98
Identity = 181/287 (63.07%), Postives = 211/287 (73.52%), Query Frame = 1


Query: 28  TPTKNNMNIIDSCWRSSSNWAKNRQALANCAVGYGKDAIGGKFGTIYVVTDPSDNPSNPK 87
           +P    MN IDSCWR  +NWA NRQ+LA+CA+G+GKDAIGGKFG +Y VTDPSD+P +PK
Sbjct: 45  SPKNVVMNTIDSCWRGKTNWASNRQSLADCAIGFGKDAIGGKFGDVYEVTDPSDDPVDPK 104

Query: 88  YGTLRYGVIQNKPLWVVFAKSMVIVLKNELMINSFKTIDGRGANVEIAYGPCITVQGVSH 147
            GTLRYG IQ +PLW+ FAK MVI LKNELM+NS+K+IDGRGA VEIA G CIT+QGVSH
Sbjct: 105 PGTLRYGAIQTEPLWITFAKDMVISLKNELMVNSYKSIDGRGAKVEIANGACITIQGVSH 164

Query: 148 VIIHGISIHDCKPAKAGLVRDTVTHVGKRRGSDGDAISIFASSHVWIDHCYLAQSTDANN 207
           VIIHGISIHDCKP   G+VR T  HVG R GSDGDAISIFASS+VWIDHC+LA+ TD   
Sbjct: 165 VIIHGISIHDCKPGDGGMVRSTPEHVGYREGSDGDAISIFASSNVWIDHCFLARCTDG-- 224

Query: 208 RYDEWKMYAIGGSANPTIFSEGNYFVAPKNSNAKQVTKREVNKGWNNWKWRSSRDVFLNG 267
                 +  I  S   TI    NYF     ++  +VTKRE ++ WNNWKWRS  D FLNG
Sbjct: 225 -----LIDVIHASTAVTI--SNNYF-----THHDKVTKRESSEKWNNWKWRSYGDEFLNG 284

Query: 268 AYFVQSGWGSCSPFYTRAQSFPVAPGSKVPALTANSGPLRCSIGKRC 315
           AYFV SG+GSCSP Y+ AQ+F     S VP LT N+GPL C + K C
Sbjct: 285 AYFVPSGYGSCSPLYSSAQTFTATQASMVPFLTLNAGPLNCVLHKPC 317

[Close]

BLAST of Cp4.1LG20g03400 vs. NCBI nr
Match: gi|659067685|ref|XP_008440763.1| (PREDICTED: putative pectate lyase 2 [Cucumis melo])

HSP 1 Score: 363.2 bits (931), Expect = 4.5e-97
Identity = 171/204 (83.82%), Postives = 184/204 (90.20%), Query Frame = 1


Query: 1   MASSSSHLLLTLLIIISLIQSSHQDYYTPTKNNMNIIDSCWRSSSNWAKNRQALANCAVG 60
           M S SSHLLL LL I S I  S Q YYTP K NMN+IDSCWR +SNWAKNR+ALA+CAVG
Sbjct: 1   MDSKSSHLLLILLFIFSFINPSLQGYYTPDKKNMNVIDSCWRQTSNWAKNRKALADCAVG 60

Query: 61  YGKDAIGGKFGTIYVVTDPSDNPSNPKYGTLRYGVIQNKPLWVVFAKSMVIVLKNELMIN 120
           YGKDAIGGKFGTIY VTDPSDNPSNPKYGTLRYGVIQ+KPLW+VF K MVIVLKNELM+N
Sbjct: 61  YGKDAIGGKFGTIYTVTDPSDNPSNPKYGTLRYGVIQDKPLWIVFGKDMVIVLKNELMVN 120

Query: 121 SFKTIDGRGANVEIAYGPCITVQGVSHVIIHGISIHDCKPAKAGLVRDTVTHVGKRRGSD 180
           SFKTIDGRGA VEIAYGPCITVQGVSHVIIHGISIHDCKP K+GLVRDTV+HVGKR+GSD
Sbjct: 121 SFKTIDGRGAKVEIAYGPCITVQGVSHVIIHGISIHDCKPGKSGLVRDTVSHVGKRKGSD 180

Query: 181 GDAISIFASSHVWIDHCYLAQSTD 205
           GDAI++F SSHVWIDHCYLA+ TD
Sbjct: 181 GDAIAVFGSSHVWIDHCYLARCTD 204

[Close]

BLAST of Cp4.1LG20g03400 vs. NCBI nr
Match: gi|703108999|ref|XP_010099179.1| (hypothetical protein L484_014178 [Morus notabilis])

HSP 1 Score: 357.8 bits (917), Expect = 1.9e-95
Identity = 189/352 (53.69%), Postives = 227/352 (64.49%), Query Frame = 1


Query: 31  KNNMNIIDSCWRSSSNWAKNRQALANCAVGYGKDAIGGKFGTIYVVTDPSDNPSNPKYGT 90
           KN MN+IDSCWR  S+WA NRQALANCA GYGKDA+GGK+G IY VT+PSD+P NPK GT
Sbjct: 25  KNIMNVIDSCWRGKSSWANNRQALANCAKGYGKDALGGKYGRIYTVTNPSDDPVNPKPGT 84

Query: 91  LRYGVIQNKPLWVVFAKSMVIVLKNELMINSFKTIDGRGANVEIAYGPCITVQGVSHVII 150
           LRYGVIQ KPLW+VFAK MVI LKNEL++NS+KTIDGRGANVEIAYGPCITVQGVSHVII
Sbjct: 85  LRYGVIQTKPLWIVFAKDMVIKLKNELIVNSYKTIDGRGANVEIAYGPCITVQGVSHVII 144

Query: 151 HGISIHDCKPAKAGLVRDTVTHVGKRRGSDGDAISIFASSHVWIDHCYLAQSTD------ 210
           HGISIHDCKP K G+VR ++THVG R G DGDAISIFASSHVWIDHC+L++S+D      
Sbjct: 145 HGISIHDCKPGKPGMVRSSLTHVGNRGGCDGDAISIFASSHVWIDHCFLSRSSDGLIDVI 204

Query: 211 --------ANNRYDEW-KMYAIGGS----ANPTI-----FSEGNYFVAPKNSNAKQVTKR 270
                   +NN + +  K+  +G S    A+ T+     F+     +  +    +     
Sbjct: 205 HASTAVTISNNFFSQHDKVMLLGHSDKFTADKTMKVTIAFNRFGPGLIERMPRVRFGYAH 264

Query: 271 EVNKGWNNWKWRSS--------------------------------------------RD 315
             N  ++ WK  +                                             +D
Sbjct: 265 VANNRYDQWKMYAVGGSANPTIFSEGNYFVAPTNSGAKEVTKREAVNKGWKNWKWRSSKD 324

[Close]

BLAST of Cp4.1LG20g03400 vs. NCBI nr
Match: gi|470114218|ref|XP_004293313.1| (PREDICTED: putative pectate lyase 2 [Fragaria vesca subsp. vesca])

HSP 1 Score: 355.1 bits (910), Expect = 1.2e-94
Identity = 191/384 (49.74%), Postives = 237/384 (61.72%), Query Frame = 1


Query: 6   SHLLLTLLIIISL------IQSSHQDYYT--PTKNNMNIIDSCWRSSSNWAKNRQALANC 65
           +++ L LL++ SL      +++++   Y+  P     N++DSCWRS+ NWA+NRQALANC
Sbjct: 2   ANISLLLLLLCSLAYNAPTLEAAYSPSYSKNPKPTTKNVVDSCWRSNPNWARNRQALANC 61

Query: 66  AVGYGKDAIGGKFGTIYVVTDPSDNPSNPKYGTLRYGVIQNKPLWVVFAKSMVIVLKNEL 125
           AVG+GKDAIGGK+G IYVVT  SD+P NPK GTLRYGVIQ +PLW+VFAK MVI LKNEL
Sbjct: 62  AVGFGKDAIGGKYGRIYVVTTSSDDPINPKPGTLRYGVIQTQPLWIVFAKDMVITLKNEL 121

Query: 126 MINSFKTIDGRGANVEIAYGPCITVQGVSHVIIHGISIHDCKPAKAGLVRDTVTHVGKRR 185
           ++NSFKTIDGRGA VEIAYGPCITVQGVSHVIIHGISIHDCKP K+G+VR T+ HVG R 
Sbjct: 122 IMNSFKTIDGRGAKVEIAYGPCITVQGVSHVIIHGISIHDCKPGKSGMVRSTLNHVGHRL 181

Query: 186 GSDGDAISIFASSHVWIDHCYLAQSTD--------------ANNRYDEW-KMYAIGGSAN 245
           GSDGD+ISIFASSHVW+DHC+L++  D               NN + +  K+  +G +  
Sbjct: 182 GSDGDSISIFASSHVWVDHCFLSRGADGLVDITHASTAVTVTNNYFSQHDKVMLLGHNDK 241

Query: 246 PTIFSEGNYFVAPKNSNAKQVTK---------REVNKGWNNWKWR--------------- 305
            T        +A     A  + +            N  ++ WK                 
Sbjct: 242 FTADKIMKVTIAFNRFGAGLIERMPRVRFGYAHVANNRYDEWKMYAIGGSANPTIFSEGN 301

Query: 306 ----------------------------SSRDVFLNGAYFVQSGWGSCSPFYTRAQSFPV 315
                                       SS+DV  NGAYF+QSGWGSC P YTR Q F V
Sbjct: 302 YFIAPTNPDAKQVTKREAYGGWKNWKWRSSKDVLQNGAYFIQSGWGSCYPMYTRTQKFKV 361

The following BLAST results are available for this feature:

BLAST of Cp4.1LG20g03400 vs. Swiss-Prot


Match Name	E-value	Identity	Description
PLY16_ARATH	3.5e-81	44.70	Probable pectate lyase 16 OS=Arabidopsis thaliana GN=At4g22080 PE=2 SV=1	[more]
PLY2_ARATH	5.9e-81	43.57	Putative pectate lyase 2 OS=Arabidopsis thaliana GN=At1g11920 PE=3 SV=2	[more]
PLY17_ARATH	1.4e-77	43.67	Putative pectate lyase 17 OS=Arabidopsis thaliana GN=At4g22090 PE=3 SV=1	[more]
PLY4_ARATH	6.3e-59	57.14	Probable pectate lyase 4 OS=Arabidopsis thaliana GN=At1g30350 PE=2 SV=1	[more]
PLY5_ARATH	8.3e-59	39.71	Probable pectate lyase 5 OS=Arabidopsis thaliana GN=At1g67750 PE=2 SV=2	[more]

BLAST of Cp4.1LG20g03400 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LU35_CUCSA	8.8e-100	85.29	Pectate lyase OS=Cucumis sativus GN=Csa_1G059200 PE=3 SV=1	[more]
A0A0S3S3R8_PHAAN	1.7e-98	63.07	Pectate lyase OS=Vigna angularis var. angularis GN=Vigan.05G085900 PE=3 SV=1	[more]
W9RY44_9ROSA	1.3e-95	53.69	Pectate lyase OS=Morus notabilis GN=L484_014178 PE=3 SV=1	[more]
M5X1K7_PRUPE	2.3e-92	50.13	Pectate lyase OS=Prunus persica GN=PRUPE_ppa025559mg PE=3 SV=1	[more]
U5FUZ6_POPTR	4.4e-91	55.02	Pectate lyase (Fragment) OS=Populus trichocarpa GN=POPTR_0011s002501g PE=3 SV=1	[more]

BLAST of Cp4.1LG20g03400 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G22080.1	1.9e-82	44.70	root hair specific 14	[more]
AT1G11920.1	3.3e-82	43.57	Pectin lyase-like superfamily protein	[more]
AT4G22090.1	7.6e-79	43.67	Pectin lyase-like superfamily protein	[more]
AT1G30350.1	3.6e-60	57.14	Pectin lyase-like superfamily protein	[more]
AT1G67750.1	4.7e-60	39.71	Pectate lyase family protein	[more]

BLAST of Cp4.1LG20g03400 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449470674\|ref\|XP_004153041.1\|	1.3e-99	85.29	PREDICTED: putative pectate lyase 2 [Cucumis sativus]	[more]
gi\|965600306\|dbj\|BAT87484.1\|	2.4e-98	63.07	hypothetical protein VIGAN_05085900 [Vigna angularis var. angularis]	[more]
gi\|659067685\|ref\|XP_008440763.1\|	4.5e-97	83.82	PREDICTED: putative pectate lyase 2 [Cucumis melo]	[more]
gi\|703108999\|ref\|XP_010099179.1\|	1.9e-95	53.69	hypothetical protein L484_014178 [Morus notabilis]	[more]
gi\|470114218\|ref\|XP_004293313.1\|	1.2e-94	49.74	PREDICTED: putative pectate lyase 2 [Fragaria vesca subsp. vesca]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR018082	AmbAllergen
IPR012334	Pectin_lyas_fold
IPR011050	Pectin_lyase_fold/virulence
IPR002022	Pec_lyase

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
biological_process	GO:0045490	pectin catabolic process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0016829	lyase activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0030570	pectate lyase activity
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG20g03400.1	Cp4.1LG20g03400.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002022	Pectate lyase/Amb allergen	PFAM	PF00544	Pec_lyase_C	coord: 114..203 score: 3.
IPR002022	Pectate lyase/Amb allergen	SMART	SM00656	amb_all	coord: 108..238 score: 1.4
IPR011050	Pectin lyase fold/virulence factor	unknown	SSF51126	Pectin lyase-like	coord: 29..308 score: 6.86
IPR012334	Pectin lyase fold	GENE3D	G3DSA:2.160.20.10		coord: 206..305 score: 2.0E-21coord: 35..204 score: 3.7
IPR018082	AmbAllergen	PRINTS	PR00807	AMBALLERGEN	coord: 57..74 score: 6.7E-38coord: 180..201 score: 6.7E-38coord: 116..132 score: 6.7E-38coord: 80..105 score: 6.7
None	No IPR available	PANTHER	PTHR31683	FAMILY NOT NAMED	coord: 34..314 score: 3.1E
None	No IPR available	PANTHER	PTHR31683:SF18	PECTATE LYASE 2-RELATED	coord: 34..314 score: 3.1E

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cp4.1LG20g03400	CmaCh13G007570	Cucurbita maxima (Rimu)	cmacpeB234
Cp4.1LG20g03400	CmoCh13G007880	Cucurbita moschata (Rifu)	cmocpeB201
Cp4.1LG20g03400	Csa6G513500	Cucumber (Chinese Long) v2	cpecuB525
Cp4.1LG20g03400	MELO3C007411.2	Melon (DHL92) v3.6.1	cpemedB573
Cp4.1LG20g03400	CsaV3_6G048810	Cucumber (Chinese Long) v3	cpecucB0650
Cp4.1LG20g03400	Bhi08G001482	Wax gourd	cpewgoB0637
Cp4.1LG20g03400	CsGy6G032880	Cucumber (Gy14) v2	cgybcpeB814
Cp4.1LG20g03400	CsGy1G010350	Cucumber (Gy14) v2	cgybcpeB070
Cp4.1LG20g03400	Carg13683	Silver-seed gourd	carcpeB1291
Cp4.1LG20g03400	Carg07558	Silver-seed gourd	carcpeB1401

The following gene(s) are paralogous to this gene:


Gene	Paralogue	Organism	Block
Cp4.1LG20g03400	Cp4.1LG10g09820	Cucurbita pepo (Zucchini)	cpecpeB086

The following block(s) are covering this gene:


Gene	Organism	Block
Cp4.1LG20g03400	Watermelon (Charleston Gray)	cpewcgB450
Cp4.1LG20g03400	Watermelon (Charleston Gray)	cpewcgB463
Cp4.1LG20g03400	Watermelon (97103) v1	cpewmB503
Cp4.1LG20g03400	Watermelon (97103) v1	cpewmB513
Cp4.1LG20g03400	Melon (DHL92) v3.5.1	cpemeB485
Cp4.1LG20g03400	Melon (DHL92) v3.5.1	cpemeB477
Cp4.1LG20g03400	Melon (DHL92) v3.6.1	cpemedB559
Cp4.1LG20g03400	Silver-seed gourd	carcpeB0697
Cp4.1LG20g03400	Silver-seed gourd	carcpeB0698
Cp4.1LG20g03400	Cucumber (Chinese Long) v3	cpecucB0624
Cp4.1LG20g03400	Cucurbita pepo (Zucchini)	cpecpeB045
Cp4.1LG20g03400	Cucumber (Gy14) v1	cgycpeB0040
Cp4.1LG20g03400	Cucumber (Gy14) v1	cgycpeB0159
Cp4.1LG20g03400	Cucurbita maxima (Rimu)	cmacpeB443
Cp4.1LG20g03400	Cucurbita maxima (Rimu)	cmacpeB677
Cp4.1LG20g03400	Cucurbita moschata (Rifu)	cmocpeB403
Cp4.1LG20g03400	Cucurbita moschata (Rifu)	cmocpeB405
Cp4.1LG20g03400	Cucurbita moschata (Rifu)	cmocpeB631
Cp4.1LG20g03400	Wild cucumber (PI 183967)	cpecpiB506
Cp4.1LG20g03400	Wild cucumber (PI 183967)	cpecpiB527
Cp4.1LG20g03400	Cucumber (Chinese Long) v2	cpecuB506
Cp4.1LG20g03400	Bottle gourd (USVL1VR-Ls)	cpelsiB415
Cp4.1LG20g03400	Bottle gourd (USVL1VR-Ls)	cpelsiB417