Cp4.1LG20g01060 (gene) Cucurbita pepo (Zucchini)

BLAST of Cp4.1LG20g01060 vs. Swiss-Prot
Match: PHRF1_RAT (PHD and RING finger domain-containing protein 1 OS=Rattus norvegicus GN=Phrf1 PE=1 SV=2)

HSP 1 Score: 82.0 bits (201), Expect = 2.4e-14
Identity = 57/183 (31.15%), Postives = 85/183 (46.45%), Query Frame = 1

Query: 12  PEDRLKALISCPSARTKSGFENESAEGEFQE-----NRPLIDDNSSSICVICFSDDGKAE 71
           PEDR        S  ++ G E  +A  E Q      + P  DD++ S C IC +      
Sbjct: 68  PEDR------SGSEDSEDGIEVPTAAVETQRKLEASSTPNSDDDAES-CPICLNAFRDQA 127

Query: 72  RGKLDSCDHYFCFVCIMEWARIESRCPACKGRFTVVHRIAKDSCRLRERIVNIPMRNQDR 131
            G  ++C HYFC  CI+EW+R  + CP  +  F  +   A+ + ++ ++   IP+ N   
Sbjct: 128 VGTPETCAHYFCLDCIIEWSRNANSCPVDRTIFKCICIRAQFNGKILKK---IPVENTRA 187

Query: 132 SVIENTTNRDPYAETCCTVCKGMEDEGLMLLCDLCDSAAHTYCVGLG-ANVPEGDWFCHD 189
              E     DP   T C VC   + E  +LLCD CD+  H  C+      VP  +WFC +
Sbjct: 188 CEDEEAEEEDP---TFCEVCGRSDREDRLLLCDGCDAGYHMECLDPPLQEVPVDEWFCPE 237

BLAST of Cp4.1LG20g01060 vs. Swiss-Prot
Match: PHRF1_MOUSE (PHD and RING finger domain-containing protein 1 OS=Mus musculus GN=Phrf1 PE=1 SV=2)

HSP 1 Score: 79.3 bits (194), Expect = 1.6e-13
Identity = 58/183 (31.69%), Postives = 86/183 (46.99%), Query Frame = 1

Query: 12  PEDRLKALISCPSARTKSGFENESAEGEFQ-----ENRPLIDDNSSSICVICFSDDGKAE 71
           PEDR        S  ++ G E  +A  E Q      + P  DD++ S C IC +      
Sbjct: 68  PEDR------SGSEDSEDGVEMATAAIETQGKLEASSVPNSDDDAES-CPICLNAFRDQA 127

Query: 72  RGKLDSCDHYFCFVCIMEWARIESRCPACKGRFTVVHRIAKDSCRLRERIVNIPMRNQDR 131
            G  ++C HYFC  CI+EW+R  + CP  +  F  +   A+ + ++ ++   IP+ N   
Sbjct: 128 VGTPETCAHYFCLDCIIEWSRNANSCPVDRTVFKCICIRAQFNGKILKK---IPVENTKA 187

Query: 132 SVIENTTNRDPYAETCCTVCKGMEDEGLMLLCDLCDSAAHTYCVGLG-ANVPEGDWFCHD 189
              E     DP   T C VC   + E  +LLCD CD+  H  C+      VP  +WFC +
Sbjct: 188 CEAE---EEDP---TFCEVCGRSDREDRLLLCDGCDAGYHMECLDPPLQEVPVDEWFCPE 234

BLAST of Cp4.1LG20g01060 vs. Swiss-Prot
Match: PHRF1_HUMAN (PHD and RING finger domain-containing protein 1 OS=Homo sapiens GN=PHRF1 PE=1 SV=3)

HSP 1 Score: 75.1 bits (183), Expect = 3.0e-12
Identity = 52/186 (27.96%), Postives = 82/186 (44.09%), Query Frame = 1

Query: 4   DSTDPFASPEDRLKALISCPSART--KSGFENESAEGEFQENRPLIDDNSSSICVICFSD 63
           D  D  AS E+ L+       +    ++  E    +G+ +       D+ +  C IC + 
Sbjct: 55  DGEDEGASEEEDLEDRSGSEDSEDDGETLLEVAGTQGKLEAAGSFNSDDDAESCPICLNA 114

Query: 64  DGKAERGKLDSCDHYFCFVCIMEWARIESRCPACKGRFTVVHRIAKDSCRLRERIVNIPM 123
                 G  ++C HYFC  CI+EW++  + CP  +  F  +   A+   ++  +   IP+
Sbjct: 115 FRDQAVGTPENCAHYFCLDCIVEWSKNANSCPVDRTLFKCICIRAQFGGKILRK---IPV 174

Query: 124 RNQDRSVIENTTNRDPYAETCCTVCKGMEDEGLMLLCDLCDSAAHTYCVGLG-ANVPEGD 183
            N   S  E     DP   T C VC   + E  +LLCD CD+  H  C+      VP  +
Sbjct: 175 ENTKASEEE----EDP---TFCEVCGRSDREDRLLLCDGCDAGYHMECLDPPLQEVPVDE 230

Query: 184 WFCHDC 187
           WFC +C
Sbjct: 235 WFCPEC 230

BLAST of Cp4.1LG20g01060 vs. Swiss-Prot
Match: YQF7_SCHPO (PHD and RING finger domain-containing protein C126.07c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=SPCC126.07c PE=3 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 8.6e-12
Identity = 49/178 (27.53%), Postives = 76/178 (42.70%), Query Frame = 1

Query: 41  QENRPLIDDNSSSICVICFSDDGKAERGKLDS--------------------CDHYFCFV 100
           +E+    D +SS  C+IC S+       + DS                    C HYF   
Sbjct: 4   EEDPKPADSDSSEECIICLSNLPNCPLDQWDSSSVPASISSTLDGLRIAKIPCGHYFHNH 63

Query: 101 CIMEWARIESRCPACKGRFTVVHRIAKDSCRLRERIVNIPMRNQDRSVIE-NTTNRDPYA 160
           C+  W R+ + CP C+  F  V  +              P+  + +SV        D  +
Sbjct: 64  CLESWCRVANTCPLCRTEFLKVDVLEFVKGPWYRA---YPVEEKTQSVANAGEPFEDEGS 123

Query: 161 ETC-CTVCKGMEDEGLMLLCDLCDSAAHTYCVGLGANVPEGDWFCHDCTISRAQHADT 197
           ETC C +C   +   ++LLCD CD A HTYC+ + A VP  +++C +C +   Q  +T
Sbjct: 124 ETCRCVICGRSDHAEVLLLCDGCDDAYHTYCLNMDA-VPIEEFYCPNCVLLNYQENET 177

BLAST of Cp4.1LG20g01060 vs. Swiss-Prot
Match: IRC20_YEAST (Uncharacterized ATP-dependent helicase IRC20 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=IRC20 PE=1 SV=1)

HSP 1 Score: 58.2 bits (139), Expect = 3.8e-07
Identity = 32/90 (35.56%), Postives = 45/90 (50.00%), Query Frame = 1

Query: 47   IDDNSSSICVICFSDDGKAERGKLDSCDHYFCFVCIMEWARIESRCPACKGRFTVVHRI- 106
            ++DN    C IC    G+ E G +  C HYFC  CI+ W R  S+CP CKG F  +  + 
Sbjct: 1231 LNDNQILSCSICL---GEVEIGAIIKCGHYFCKSCILTWLRAHSKCPICKG-FCSISEVY 1290

Query: 107  ---AKDSCRLRERIVNIPMRNQDRSVIENT 133
                K+S   RE+ +  P R    S  +N+
Sbjct: 1291 NFKFKNSTEKREKEIQEPRREGADSSQDNS 1316

BLAST of Cp4.1LG20g01060 vs. TrEMBL
Match: A0A0A0LQB4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G039990 PE=4 SV=1)

HSP 1 Score: 936.0 bits (2418), Expect = 2.3e-269
Identity = 481/613 (78.47%), Postives = 530/613 (86.46%), Query Frame = 1

Query: 1   MADDSTDPFASP-EDRLKALISCPSARTKSGFENESAEGEFQENRPLIDDNSSSICVICF 60
           MADDST+ FAS  EDRLKALISCPSA  K G E E  EG F+E+  LIDDN SSICVICF
Sbjct: 48  MADDSTNSFASSSEDRLKALISCPSASAKFGNEEEFLEGGFEESHLLIDDNPSSICVICF 107

Query: 61  SDDGKAERGKLDSCDHYFCFVCIMEWARIESRCPACKGRFTVVHRIAKDSCRLRERIVNI 120
           S+DGKAERGKLDSCDHYFCFVCIMEWARIESRCPACKGRFTVVHRIAKD CRLRERIVNI
Sbjct: 108 SEDGKAERGKLDSCDHYFCFVCIMEWARIESRCPACKGRFTVVHRIAKDPCRLRERIVNI 167

Query: 121 PMRNQDRSVIENTTNRDPYAETCCTVCKGMEDEGLMLLCDLCDSAAHTYCVGLGANVPEG 180
           PMRNQD+S   +    DPYAETCCTVCKGMEDEGLMLLCDLCDSAAHT+CVGLGANVPEG
Sbjct: 168 PMRNQDQSASGSARIGDPYAETCCTVCKGMEDEGLMLLCDLCDSAAHTFCVGLGANVPEG 227

Query: 181 DWFCHDCTISRAQHADTELNTGYNDQNQTESVRAHISISDIVNESSAQTVGIPRRIALLH 240
           DW+CHDCTI+RAQH DTEL+T +  QNQT +V   I+ISDIV ESSAQTV I RR ALLH
Sbjct: 228 DWYCHDCTIARAQHTDTELDTSFIKQNQTTTVEPRIAISDIVKESSAQTVSITRRGALLH 287

Query: 241 SSHEPPSIVPSWRSRSSVAHKSLPSRGGRAAGTSARTLHRCRNIHSHIRALRENWGGLRR 300
           S+ EPPSIVPS  SR SVA KSLPSRGG+AAGTSARTLHRCRNIHS+IRALR+NW  +R+
Sbjct: 288 SNREPPSIVPS--SRRSVAQKSLPSRGGKAAGTSARTLHRCRNIHSYIRALRDNWDAVRK 347

Query: 301 GSLSFPASSSSTYCSNSSKRDIGAA--VNNQTGEPHFSTAINMQATSGPESNGSSYDVEK 360
           GSL FPASSSSTYC NSSKRD G A  +N QT +PH   AI++QAT+ PESNG+S DVEK
Sbjct: 348 GSLRFPASSSSTYCGNSSKRDTGGAELINKQTNQPH---AISLQATTLPESNGNSCDVEK 407

Query: 361 AWTMMEIAKAKAIREGDQTLKHPASRQMNTKIKDIHTAKGQQFKIQKLKEPATHKKEMGK 420
           AW MMEIAKAK+IREGDQTL+HP SRQ+ TK KD HTAKGQQ+KI+K  EPA HKKEMGK
Sbjct: 408 AWRMMEIAKAKSIREGDQTLRHPVSRQITTKTKDSHTAKGQQYKIKKPDEPAKHKKEMGK 467

Query: 421 QRLPELDKKLCCPVASKKSKASDEFLPTSSSSYGEPSLGNMVQTSRGDFNYENRRKLSNK 480
           QRL ELDK+LC PVASKK+KASDEF PT SSS GE  LG MVQT  GDF+ ENRRK SNK
Sbjct: 468 QRLLELDKQLCRPVASKKNKASDEFFPTHSSSNGEFPLGKMVQTGGGDFHCENRRKPSNK 527

Query: 481 IVDELSSSSTMTQASEKGHAAREAGNDHDPKSEIRTLVKINLKLLSRDKNLGYERYKEVA 540
           IVDE+SSSSTMTQAS+K H+AREAG+DHD KSEIRTLVKINLKLLS+ KNLGYERYKEV 
Sbjct: 528 IVDEVSSSSTMTQASDKHHSAREAGSDHDGKSEIRTLVKINLKLLSQGKNLGYERYKEVT 587

Query: 541 RLATHTIMAACGLEPPPKPSKRFLSCSVCKHTEEEVGQLHRSTLMPESCRECFLVYVKDV 600
           RLATHTIMA CGLEPPPKP+K+++S SVCKHTEEE+ +LHRSTLMP+SCR+CF+ +VKDV
Sbjct: 588 RLATHTIMARCGLEPPPKPTKQYVSSSVCKHTEEEIRKLHRSTLMPDSCRKCFMWFVKDV 647

Query: 601 VNALMLEKLEVGS 611
           VNA+MLEKL VGS
Sbjct: 648 VNAIMLEKLTVGS 655

BLAST of Cp4.1LG20g01060 vs. TrEMBL
Match: A0A0D2PV68_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_001G066400 PE=4 SV=1)

HSP 1 Score: 370.9 bits (951), Expect = 2.9e-99
Identity = 261/686 (38.05%), Postives = 353/686 (51.46%), Query Frame = 1

Query: 1   MADDSTDPFASPEDRLKALISCPSARTKSGFENESAEGEFQENRPLIDDNSSSICVICFS 60
           MA DST  F SP    +   S  S        +   E + Q+     DD+   IC IC S
Sbjct: 15  MAGDSTTEFPSPSPNKRLKTSSKSIIEDEPGNHHQPEQQRQQQAADDDDSDPEICGICLS 74

Query: 61  DDGK-AERGKLDSCDHYFCFVCIMEWARIESRCPACKGRFTVVHRIAKDSCRLRERIVNI 120
           D G+ A RGK++SCDH+FCFVCIMEWA++ESRCP CK RFTV+HR  K+     +R+VNI
Sbjct: 75  DGGRRAVRGKINSCDHFFCFVCIMEWAKVESRCPMCKRRFTVIHRPPKEGVFASQRLVNI 134

Query: 121 PMRNQDRSVIENTTN--RDPYAETCCTVCKGMEDEGLMLLCDLCDSAAHTYCVGLGANVP 180
           P R+Q   +  N T+   DPY+E  C++C GM DE L+LLCDLCDSAAHTYCVGLGA VP
Sbjct: 135 PQRDQVYHLSGNATSGLSDPYSEVKCSMCHGMTDESLLLLCDLCDSAAHTYCVGLGATVP 194

Query: 181 EGDWFCHDCTISRAQHADTELNT---------GYNDQNQTESVRAHISISDIVNESSAQT 240
           +GDWFCHDC +SR++H  T+++T          YN Q  +E     +SI DIV +S+   
Sbjct: 195 DGDWFCHDCALSRSEHEKTDVDTETDNHMIAANYNVQLPSEE---DVSIFDIVRDSNITP 254

Query: 241 VGIPRRIALLHSSHEPPSIVPSWRSRSSVAHKSLPSRG--GRAAGTSARTLHRCRNIHSH 300
           +          S+H PPSI+P   S S     S PSR   G++  + ARTL RCRN+ S 
Sbjct: 255 LEGHNASVSSCSNHSPPSIIPGCES-SGADEVSRPSRNTVGKSTESGARTLRRCRNVQSR 314

Query: 301 IRALRENWGGLRRGSLSFPASSSSTYCSNSSKRDIGAAVNNQTGEPHFSTAINMQATSGP 360
           I+ LRENW  L+  SLSF +  + +   +  K  + A  N  + E   S + + Q+TS  
Sbjct: 315 IQVLRENWRALQNRSLSFSSRMAESGGGSCGKGKVAAVCNPGSSESQPSISTSQQSTSRD 374

Query: 361 ES---NGSSYDVEKAWTMMEIAKA----------------------KAIREGDQTLKHPA 420
            S       YD++KAW MM IAK+                       A +E  ++  H  
Sbjct: 375 SSIQPKEDLYDIDKAWKMMNIAKSMQKNCKTTNSLNRNSTKLPCLGSASKEAIRSSSHHI 434

Query: 421 SRQMNTKIKDIHTAKGQQFKIQKLKEPATHKKEMGKQRLPELDKKLCCPVASKKSKASDE 480
                 KI+   T   ++   QK      HK+E  K + PEL+K+    + ++ S    E
Sbjct: 435 -----LKIQQNETRNEERMGKQKQYGYHCHKREKEKCKSPELEKQKRVVINTQPS----E 494

Query: 481 FLPTSSS-SYGEPSLGNMVQTSRGDFNYENRRKLSNKIVDELSSSSTMTQASEKGHAAR- 540
            +P+S S  + +PS    VQ         +      KI       S+ T  +E G  +  
Sbjct: 495 RVPSSHSLHFFQPSSSANVQIENSCSRVNDLGPFV-KIAQSRCRESSSTANTEGGSCSTS 554

Query: 541 -----------------EAG-NDHDP----------------------KSEIRTLVKINL 600
                            EAG N  D                       KSEI+ LVK+NL
Sbjct: 555 LFGSVLRGSMDFLDSNLEAGVNKLDIPEARTRLKKSCSKSKDRKDDNAKSEIQALVKLNL 614

Query: 601 KLLSRDKNLGYERYKEVARLATHTIMAACGLEPPPKPSKRFLSCSVCKHTEEEVGQLHRS 606
           KLLS+DK LG   +KE+ARLATHTI+AA G +   K S       VC H+  ++   H+S
Sbjct: 615 KLLSQDKRLGVVIFKEIARLATHTILAAYGFQ-HSKSSIHSFPSPVCSHS-NDINLQHKS 674

BLAST of Cp4.1LG20g01060 vs. TrEMBL
Match: D7SIQ0_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_17s0000g04770 PE=4 SV=1)

HSP 1 Score: 361.3 bits (926), Expect = 2.3e-96
Identity = 246/634 (38.80%), Postives = 337/634 (53.15%), Query Frame = 1

Query: 49  DNSSSICVICFSDDGKAERGKLDSCDHYFCFVCIMEWARIESRCPACKGRFTVVHRIAKD 108
           D  S+ C IC  +D +A RG +DSCDHYFCFVCIMEWA++ESRCP CK RF+ + R  K 
Sbjct: 47  DFDSNCCGICLLEDDRAIRGWVDSCDHYFCFVCIMEWAKVESRCPMCKRRFSTIRRPPKP 106

Query: 109 SCRLRERIVNIPMRNQDRSVIENTTN--RDPYAETCCTVCKGMEDEGLMLLCDLCDSAAH 168
                ERIVN+P+R+Q      N T    DPYAE  C +C G  DE  +L+CDLCDSAAH
Sbjct: 107 GVFPSERIVNVPVRDQAYHHFGNVTTGPSDPYAEVDCGICHGTADESFLLICDLCDSAAH 166

Query: 169 TYCVGLGANVPEGDWFCHDCTISRAQHADTELNTGYNDQN------QTESVRAHISISDI 228
           TYCVGLG  VPEGDWFCHDCT+SRA+HA+ E++  ++D+N      +  S   H+SI DI
Sbjct: 167 TYCVGLGHTVPEGDWFCHDCTVSRAEHANGEIDAVFDDRNNFRNFYRKPSAETHVSICDI 226

Query: 229 VNESSAQTVGIPRRIALLHSSHEPPSIVPSWRSRSSVAHKSLPSRGGRAAGTSARTLHRC 288
           V E   +    P  I   H +   PS   +  S +   + S       ++ + ARTL RC
Sbjct: 227 VAEPYTREDERPPSIGSSHPNR--PSSPIALNSENFAVNNS-------SSMSVARTLLRC 286

Query: 289 RNIHSHIRALRENWGGLRRGSLSFPASSSSTYCSNSSKRDIGAAVNNQTGEPHFSTAINM 348
           RN+++HI+ALRENW  LR GS++F +    +   +S K ++GA+   Q            
Sbjct: 287 RNVNAHIQALRENWNALRSGSMTFSSILGDSGGESSQKYNVGASCPKQ------------ 346

Query: 349 QATSGPESNGSSYDVEKAWTMMEIAKAKAIREG-------DQTLKHPASRQMNTKIKDIH 408
                 ++N  SYD EKAW MM+  KAK+I+          Q  KHP  + +N   K++ 
Sbjct: 347 ------DNNACSYDTEKAWKMMD--KAKSIQRACERTGIIHQVSKHPLGK-VNVP-KEVA 406

Query: 409 TAKGQQFKIQKLKEPATHKKEMG--------KQRLPELDKKLCCPVAS---KKSKASDEF 468
             K            AT+ +++G        K    E D K   P  S   ++ + +   
Sbjct: 407 NVKSSS---------ATNNRQLGNKDFGWHFKHHSLETDNKRYKPQKSENQEQGRVTRTG 466

Query: 469 LP--TSSS-------SYGEPSLGNMVQTSRGDFNYENRRKLSNK-IVDELSS-------- 528
           +P  T SS       S+  PS      + + D ++    + S K +VD  ++        
Sbjct: 467 IPKFTESSLTIHSLVSFDSPSSRKAETSFQVDVHHRTGVRPSQKNLVDSFNAINERDGYG 526

Query: 529 -----------SSTMTQASEKGHAA---------------------REAGNDHDPKSEIR 588
                      S  ++ A+ K  A+                      +A  D D KSEI+
Sbjct: 527 SLIIPDGPVPRSPDLSHANLKFGASSLCKEDLLHRPKGRVEIGSAKNKARRDDDAKSEIQ 586

Query: 589 TLVKINLKLLSRDKNLGYERYKEVARLATHTIMAACGLEPPPKPSKRFLSCSVCKHTEEE 607
           +LVK+NLKL+S+DK LG + +KEVARLATHTI+AACGLE   K   R    S+C H+ E+
Sbjct: 587 SLVKLNLKLISKDKQLGVDTFKEVARLATHTILAACGLE-HSKSCVRSFPTSMCTHS-EQ 638

BLAST of Cp4.1LG20g01060 vs. TrEMBL
Match: A0A0V0IKP2_SOLCH (Putative PHD and RING finger domain-containing protein-like OS=Solanum chacoense PE=4 SV=1)

HSP 1 Score: 331.3 bits (848), Expect = 2.6e-87
Identity = 241/661 (36.46%), Postives = 340/661 (51.44%), Query Frame = 1

Query: 8   PFASPEDRLKALI-----SCPSARTKSGFENESAEGEFQENRPLIDDNSSSICVICFSDD 67
           P   P  R+K L      S PS+  K   + E ++ E + N           C IC ++ 
Sbjct: 11  PEMYPSKRVKTLTLGSPESSPSSSDKGKSKLELSDSETESN----------CCGICLTET 70

Query: 68  GKAE---RGKLDSCDHYFCFVCIMEWARIESRCPACKGRFTVVHRIAKDSCRLRERIVNI 127
           G      RG +D CDHYFCFVCI+EW+++ESRCP CK RF+++ R  +      ER+VN+
Sbjct: 71  GHGTTIIRGVIDCCDHYFCFVCIIEWSKVESRCPMCKRRFSIIRRPPRPPIFPSERVVNV 130

Query: 128 PMRNQDRSVIENTTN--RDPYAETCCTVCKGMEDEGLMLLCDLCDSAAHTYCVGLGANVP 187
           P+R+Q      N T   RD YAE  C VC    D+ L+LLCDLCD+A HTYCVGLGA VP
Sbjct: 131 PVRDQVYHCHGNATTGPRDLYAEVQCNVCHSTADDVLLLLCDLCDTAYHTYCVGLGATVP 190

Query: 188 EGDWFCHDCTISRAQHADTELNT------GYNDQNQTESVRAHISISDIVNESSAQTVGI 247
           EGDWFC DC + +A+ A +E+ T       +++    ES R  +SI DIV ES+   V  
Sbjct: 191 EGDWFCADCALLKAEQAQSEIKTDCYTQTSFSEPQSAESAR-QVSIYDIVRESNVDGVES 250

Query: 248 PRRIAL--LHSSHEPPSIVPSWRSRSSVAHKSLPSRG--GRAAGTSARTLHRCRNIHSHI 307
               AL    SS    S   S    SS+   S+ + G  GRA   +ARTL  CRN+H  I
Sbjct: 251 APNGALDASDSSSRIESRRQSTPRSSSIRLGSVGNNGVAGRANKLNARTLGHCRNVHDRI 310

Query: 308 RALRENWGGLRRGSLSFPASSSSTYCSNSSKRDIGAAVNNQTGEPHFSTAINMQATSGP- 367
           R LRE+W G + GSL FP+S      SN+S   +     + +GE    +  +   TS P 
Sbjct: 311 RILREHWNGFQSGSLRFPSSK----VSNASSAKLKPVRGSSSGEHPLVSCSSQPRTSQPS 370

Query: 368 -----ESNGSSYDVEKAWTMMEIAKA-----KAIREGDQTLKHPASRQMNTKIKDIHTAK 427
                +S GS+ +VEKAW MM +AKA        +   Q  KH  S Q NT  K+   + 
Sbjct: 371 NQNRPDSKGST-EVEKAWKMMNMAKAIEQNSVGNKSSVQASKH--SVQKNTSYKEASESS 430

Query: 428 GQQFKIQKLKEPATHKKEMGKQRLPELD------------------KKLCCP-----VAS 487
            +   ++  +   T+ + +G     E D                     C P     V+S
Sbjct: 431 SRSISLKIEQHRPTYIQNVGPGSHSESDVCKLDIRSNGASGVYNGSSMSCRPLYSELVSS 490

Query: 488 KKSKAS--------DEFLPTSSSSY-GEPSLGNMVQTSRGDFNYENRRKLSNKIVDELSS 547
           K+S+ S        +  LP  + S  G   L + ++++       + +++S+     ++ 
Sbjct: 491 KESQTSLQPYVCDANNGLPRKNKSCAGSTHLSSSIRSTSISTTVGHEKEISSSSNSNVNP 550

Query: 548 SSTMTQASEKGHAAREAGNDHDPKSEIRTLVKINLKLLSRDKNLGYERYKEVARLATHTI 606
              +    EK    R      D K+EI++LVK+NLK+L RDK L  + +K+VARLATH+I
Sbjct: 551 QEKLKV--EKSGTKRRVEVKSDAKTEIQSLVKLNLKILCRDKQLEVDTFKQVARLATHSI 610

BLAST of Cp4.1LG20g01060 vs. TrEMBL
Match: M0ZXY8_SOLTU (Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG400004060 PE=4 SV=1)

HSP 1 Score: 328.9 bits (842), Expect = 1.3e-86
Identity = 244/668 (36.53%), Postives = 342/668 (51.20%), Query Frame = 1

Query: 8   PFASPEDRLKALI-----SCPSARTKSGFENESAEGEFQENRPLIDDNSSSICVICFSDD 67
           P   P  R+K L      S PS+  K   + E ++ E + N           C IC ++ 
Sbjct: 11  PEMYPSKRVKTLTLGSPESSPSSSDKGKSKLELSDSETESN----------CCGICLTET 70

Query: 68  GKAE---RGKLDSCDHYFCFVCIMEWARIESRCPACKGRFTVVHRIAKDSCRLRERIVNI 127
           G      RG +D CDHYFCFVCI+EW+++ESRCP CK RF+++ R  +      ER+VN+
Sbjct: 71  GHGTTIIRGVIDCCDHYFCFVCIIEWSKVESRCPMCKRRFSIIRRPPRPPIFPSERVVNV 130

Query: 128 PMRNQDRSVIENTTN--RDPYAETCCTVCKGMEDEGLMLLCDLCDSAAHTYCVGLGANVP 187
           P+R+Q      N T   RD YAE  C VC    D+ L+LLCDLCD+A HTYCVGLGA VP
Sbjct: 131 PVRDQVYHCHGNATTGPRDLYAEVQCNVCHSTADDVLLLLCDLCDTAYHTYCVGLGATVP 190

Query: 188 EGDWFCHDCTISRAQHADTELNT------GYNDQNQTESVRAHISISDIVNESSAQTVGI 247
           EGDWFC DC + +A+ A +E+ T       +++    ES R  +SI DIV ES    V  
Sbjct: 191 EGDWFCADCALLKAEQAQSEIKTDCYTQTSFSEPQSAESAR-QVSIYDIVRESYVDGVES 250

Query: 248 PRRIALLHSSHEPPSIVPSWR---SRSSVAHKSLPSRGG---RAAGTSARTLHRCRNIHS 307
               AL   + E  S + S R   SRSS        + G   RA   +ARTL  CRN+H 
Sbjct: 251 APNGAL--DASESSSRIESRRQSTSRSSSIRLGYVGKNGVAGRATKFNARTLGHCRNVHD 310

Query: 308 HIRALRENWGGLRRGSLSFPASSSSTYCSNSSKRDIGAAVNNQTGEPHFSTAINMQATSG 367
            IR LRE+W G + GSL FP+S      SN+S   +     + +GE    +  +   TS 
Sbjct: 311 RIRILREHWNGFQSGSLRFPSSK----VSNASSAKLKPVRGSSSGERPLVSCSSQPRTSQ 370

Query: 368 P------ESNGSSYDVEKAWTMMEIAKA-----KAIREGDQTLKHPASRQMNTKIKDIHT 427
           P      +S GS+ +VEKAW MM +AKA        +   Q  KH  S Q NT  K+   
Sbjct: 371 PSNQNRPDSKGST-EVEKAWKMMNMAKAIEQNSVGNKSSVQASKH--SVQKNTSYKEASE 430

Query: 428 AKGQQFKIQKLKEPATHKKEMGKQRLPELD------------------KKLCCP-----V 487
           +  +   ++  +   T+ + +G     E D                     C P     V
Sbjct: 431 SSSRSISLKIEQHRPTYIQNVGPGSHSESDVCKLDIRSKGASGVYNGSSMSCRPLYSELV 490

Query: 488 ASKKSKAS--------DEFLPTSSSSY-GEPSLGNMVQTSRGDFNYENRRKLSNKIVDEL 547
           +SK+S+ S        +  LP  + S  G   L + ++++       + +++S       
Sbjct: 491 SSKESQTSLQPYVCDANNGLPRKNKSCAGSTHLSSSIRSTSISTTVGHEKEIS------- 550

Query: 548 SSSSTMTQASEKGHAAREAGNDH-----DPKSEIRTLVKINLKLLSRDKNLGYERYKEVA 606
           SSS+++    EK    R           D K+EI++LVK+NLK+L RDK L  + +K+VA
Sbjct: 551 SSSNSIVNPQEKLKVERSGTKRRVEVKSDAKTEIQSLVKLNLKILCRDKKLEADTFKQVA 610

BLAST of Cp4.1LG20g01060 vs. TAIR10
Match: AT3G05670.1 (AT3G05670.1 RING/U-box protein)

HSP 1 Score: 152.9 bits (385), Expect = 6.3e-37
Identity = 92/249 (36.95%), Postives = 130/249 (52.21%), Query Frame = 1

Query: 49  DNSSSICVICFSD-DGKAERGKLDSCDHYFCFVCIMEWARIESRCPACKGRFTVVHRIAK 108
           D   ++C IC S+ D +  +G LD C HYFCF CIMEW+++ESRCP CK RF  + + A+
Sbjct: 407 DVIKNVCGICLSEEDMRRLKGTLDCCSHYFCFTCIMEWSKVESRCPLCKQRFRTISKPAR 466

Query: 109 DS--CRLRERIVNIPMRNQDRSVIENTTNR--DPYAETCCTVCKGMEDEGLMLLCDLCDS 168
            +    LRE ++ +P R+Q     E       DPY    CT C   +D+GLMLLCDLCDS
Sbjct: 467 STPGVDLREVVIPVPERDQVYQPTEEELRSYLDPYENIICTECHQGDDDGLMLLCDLCDS 526

Query: 169 AAHTYCVGLGANVPEGDWFCHDCTISRAQHADTELNTGYNDQNQTESVRAHISISDIVNE 228
           +AHTYCVGLG  VPEG+W+C  C       A           +QT  +      S   + 
Sbjct: 527 SAHTYCVGLGREVPEGNWYCEGCRPVALGSA----------SSQTHIISEQQRGSGFYSR 586

Query: 229 SSAQTV-GIPRRIALLHSSHEPPSIVPSWRSRSSVAHKSLP---SRGGRAAGTSARTLHR 288
            S   V G  + ++L+ S   P      + +  ++    +P   ++G   +G  A TL R
Sbjct: 587 PSPLVVSGQYQDVSLIVSPRTP------FFNGENLFSPRIPNGDAQGSSPSGLGATTLSR 639

BLAST of Cp4.1LG20g01060 vs. TAIR10
Match: AT4G10940.1 (AT4G10940.1 RING/U-box protein)

HSP 1 Score: 51.2 bits (121), Expect = 2.6e-06
Identity = 21/48 (43.75%), Postives = 28/48 (58.33%), Query Frame = 1

Query: 55  CVICFSDDGKAERGKLDSCDHYFCFVCIMEWARIESRCPACKGRFTVV 103
           C IC   D   +RG LD C H+FCF CI  W+ I + CP C+  F ++
Sbjct: 31  CGICM--DIIIDRGVLDCCQHWFCFECIDNWSTIMNLCPLCQREFQLI 76

BLAST of Cp4.1LG20g01060 vs. TAIR10
Match: AT4G26400.2 (AT4G26400.2 RING/U-box superfamily protein)

HSP 1 Score: 49.7 bits (117), Expect = 7.5e-06
Identity = 26/90 (28.89%), Postives = 37/90 (41.11%), Query Frame = 1

Query: 36  AEGEFQENRPLIDDNSSSICVICFSDDGKAERGKLDSCDHYFCFVCIMEWARIESRCPAC 95
           A  E  +N P +  + S  C IC  D  K    K   C H F   CI+ W  + S CP C
Sbjct: 222 ARKEVVDNLPTVKISESLQCSICLDDFDKGSEAKEMPCKHKFHIRCIVPWLELHSSCPVC 281

Query: 96  KGRFTVVHRIAKDSCRLRERIVNIPMRNQD 126
           +           +  R R R + I + N++
Sbjct: 282 RYELPPDDETKVNPVRPRTRTLEINVSNEN 311

BLAST of Cp4.1LG20g01060 vs. NCBI nr
Match: gi|659066655|ref|XP_008454700.1| (PREDICTED: uncharacterized protein LOC103495032 [Cucumis melo])

HSP 1 Score: 945.3 bits (2442), Expect = 5.4e-272
Identity = 488/617 (79.09%), Postives = 534/617 (86.55%), Query Frame = 1

Query: 1   MADDSTDPFAS-PEDRLKALISCPSARTKSGFENESAEGEFQENRPLIDDNSSSICVICF 60
           MADDST+ FAS PEDRLKALISCPSA  KSG ENE  EG F+EN  LIDDN SSICVICF
Sbjct: 1   MADDSTNSFASSPEDRLKALISCPSASAKSGDENELPEGGFEENHLLIDDNHSSICVICF 60

Query: 61  SDDGKAERGKLDSCDHYFCFVCIMEWARIESRCPACKGRFTVVHRIAKDSCRLRERIVNI 120
           SDDGKAERGKLDSCDHYFCFVCIMEWARIESRCPACKGRFTVVHRIAKD CRLRERIVNI
Sbjct: 61  SDDGKAERGKLDSCDHYFCFVCIMEWARIESRCPACKGRFTVVHRIAKDPCRLRERIVNI 120

Query: 121 PMRNQDRSVIENTTNRDPYAETCCTVCKGMEDEGLMLLCDLCDSAAHTYCVGLGANVPEG 180
           PMRNQD+S   N   RDPYAETCCTVCKGMEDEGLMLLCDLCDSAAHT+CVGLGANVPEG
Sbjct: 121 PMRNQDQSASGNARIRDPYAETCCTVCKGMEDEGLMLLCDLCDSAAHTFCVGLGANVPEG 180

Query: 181 DWFCHDCTISRAQHADTELNTGYNDQNQTESVRAHISISDIVNESSAQTVGIPRRIALLH 240
           DWFCHDCTISRAQH D +L+T +N+QNQT +V   I+ISDIV ESS+QTVGI  R ALLH
Sbjct: 181 DWFCHDCTISRAQHTDIKLDTSFNNQNQTTTVEPRIAISDIVKESSSQTVGITCRGALLH 240

Query: 241 SSHEPPSIVPSWRSRSSVAHKSLPSRGGRAAGT----SARTLHRCRNIHSHIRALRENWG 300
           S+ E PSIVPS  SRSSVA KSLPSRGG+AAGT    SARTLHRCRNIHS+IRALR+NW 
Sbjct: 241 SNRESPSIVPS--SRSSVAQKSLPSRGGKAAGTGARTSARTLHRCRNIHSYIRALRDNWD 300

Query: 301 GLRRGSLSFPASSSSTYCSNSSKRDIGAA--VNNQTGEPHFSTAINMQATSGPESNGSSY 360
            +RRGSL FPASSSSTYC NSSKRD G A  +N QT +P    AI++QAT+ PE NG+SY
Sbjct: 301 AVRRGSLKFPASSSSTYCGNSSKRDTGGAELINKQTDQPQ---AISLQATALPECNGNSY 360

Query: 361 DVEKAWTMMEIAKAKAIREGDQTLKHPASRQMNTKIKDIHTAKGQQFKIQKLKEPATHKK 420
           DVEKAW MMEIAKAKAIREGDQTL+HP SRQ+ TK KD HTAKGQQ+KI+K  EPA HKK
Sbjct: 361 DVEKAWRMMEIAKAKAIREGDQTLRHPVSRQITTKTKDSHTAKGQQYKIKKPDEPAKHKK 420

Query: 421 EMGKQRLPELDKKLCCPVASKKSKASDEFLPTSSSSYGEPSLGNMVQTSRGDFNYENRRK 480
           EMGKQRL ELDK+LC PVASKK+KASDEF PT+SSS GE  LG MVQT  GDF+ ENRRK
Sbjct: 421 EMGKQRLLELDKQLCRPVASKKNKASDEFFPTNSSSNGEFPLGKMVQTCGGDFHCENRRK 480

Query: 481 LSNKIVDELSSSSTMTQASEKGHAAREAGNDHDPKSEIRTLVKINLKLLSRDKNLGYERY 540
            SNKIVDE+SSSSTMTQAS+K H AREAGND D KSEIRTLVKINLKLLS+ KNLGYERY
Sbjct: 481 PSNKIVDEVSSSSTMTQASDKHHPAREAGNDDDGKSEIRTLVKINLKLLSQGKNLGYERY 540

Query: 541 KEVARLATHTIMAACGLEPPPKPSKRFLSCSVCKHTEEEVGQLHRSTLMPESCRECFLVY 600
           KEV+RLATH IMA CGLEPPPKP+K+++SCSVC+HTEEE+ +LHRSTLMP+SCR+CF+ +
Sbjct: 541 KEVSRLATHAIMARCGLEPPPKPTKQYVSCSVCRHTEEEIRKLHRSTLMPDSCRKCFMGF 600

Query: 601 VKDVVNALMLEKLEVGS 611
           VKDVVNA+MLEKL VGS
Sbjct: 601 VKDVVNAIMLEKLMVGS 612

BLAST of Cp4.1LG20g01060 vs. NCBI nr
Match: gi|778657168|ref|XP_004137454.2| (PREDICTED: uncharacterized protein LOC101208450 [Cucumis sativus])

HSP 1 Score: 936.0 bits (2418), Expect = 3.3e-269
Identity = 481/613 (78.47%), Postives = 530/613 (86.46%), Query Frame = 1

Query: 1   MADDSTDPFASP-EDRLKALISCPSARTKSGFENESAEGEFQENRPLIDDNSSSICVICF 60
           MADDST+ FAS  EDRLKALISCPSA  K G E E  EG F+E+  LIDDN SSICVICF
Sbjct: 1   MADDSTNSFASSSEDRLKALISCPSASAKFGNEEEFLEGGFEESHLLIDDNPSSICVICF 60

Query: 61  SDDGKAERGKLDSCDHYFCFVCIMEWARIESRCPACKGRFTVVHRIAKDSCRLRERIVNI 120
           S+DGKAERGKLDSCDHYFCFVCIMEWARIESRCPACKGRFTVVHRIAKD CRLRERIVNI
Sbjct: 61  SEDGKAERGKLDSCDHYFCFVCIMEWARIESRCPACKGRFTVVHRIAKDPCRLRERIVNI 120

Query: 121 PMRNQDRSVIENTTNRDPYAETCCTVCKGMEDEGLMLLCDLCDSAAHTYCVGLGANVPEG 180
           PMRNQD+S   +    DPYAETCCTVCKGMEDEGLMLLCDLCDSAAHT+CVGLGANVPEG
Sbjct: 121 PMRNQDQSASGSARIGDPYAETCCTVCKGMEDEGLMLLCDLCDSAAHTFCVGLGANVPEG 180

Query: 181 DWFCHDCTISRAQHADTELNTGYNDQNQTESVRAHISISDIVNESSAQTVGIPRRIALLH 240
           DW+CHDCTI+RAQH DTEL+T +  QNQT +V   I+ISDIV ESSAQTV I RR ALLH
Sbjct: 181 DWYCHDCTIARAQHTDTELDTSFIKQNQTTTVEPRIAISDIVKESSAQTVSITRRGALLH 240

Query: 241 SSHEPPSIVPSWRSRSSVAHKSLPSRGGRAAGTSARTLHRCRNIHSHIRALRENWGGLRR 300
           S+ EPPSIVPS  SR SVA KSLPSRGG+AAGTSARTLHRCRNIHS+IRALR+NW  +R+
Sbjct: 241 SNREPPSIVPS--SRRSVAQKSLPSRGGKAAGTSARTLHRCRNIHSYIRALRDNWDAVRK 300

Query: 301 GSLSFPASSSSTYCSNSSKRDIGAA--VNNQTGEPHFSTAINMQATSGPESNGSSYDVEK 360
           GSL FPASSSSTYC NSSKRD G A  +N QT +PH   AI++QAT+ PESNG+S DVEK
Sbjct: 301 GSLRFPASSSSTYCGNSSKRDTGGAELINKQTNQPH---AISLQATTLPESNGNSCDVEK 360

Query: 361 AWTMMEIAKAKAIREGDQTLKHPASRQMNTKIKDIHTAKGQQFKIQKLKEPATHKKEMGK 420
           AW MMEIAKAK+IREGDQTL+HP SRQ+ TK KD HTAKGQQ+KI+K  EPA HKKEMGK
Sbjct: 361 AWRMMEIAKAKSIREGDQTLRHPVSRQITTKTKDSHTAKGQQYKIKKPDEPAKHKKEMGK 420

Query: 421 QRLPELDKKLCCPVASKKSKASDEFLPTSSSSYGEPSLGNMVQTSRGDFNYENRRKLSNK 480
           QRL ELDK+LC PVASKK+KASDEF PT SSS GE  LG MVQT  GDF+ ENRRK SNK
Sbjct: 421 QRLLELDKQLCRPVASKKNKASDEFFPTHSSSNGEFPLGKMVQTGGGDFHCENRRKPSNK 480

Query: 481 IVDELSSSSTMTQASEKGHAAREAGNDHDPKSEIRTLVKINLKLLSRDKNLGYERYKEVA 540
           IVDE+SSSSTMTQAS+K H+AREAG+DHD KSEIRTLVKINLKLLS+ KNLGYERYKEV 
Sbjct: 481 IVDEVSSSSTMTQASDKHHSAREAGSDHDGKSEIRTLVKINLKLLSQGKNLGYERYKEVT 540

Query: 541 RLATHTIMAACGLEPPPKPSKRFLSCSVCKHTEEEVGQLHRSTLMPESCRECFLVYVKDV 600
           RLATHTIMA CGLEPPPKP+K+++S SVCKHTEEE+ +LHRSTLMP+SCR+CF+ +VKDV
Sbjct: 541 RLATHTIMARCGLEPPPKPTKQYVSSSVCKHTEEEIRKLHRSTLMPDSCRKCFMWFVKDV 600

Query: 601 VNALMLEKLEVGS 611
           VNA+MLEKL VGS
Sbjct: 601 VNAIMLEKLTVGS 608

BLAST of Cp4.1LG20g01060 vs. NCBI nr
Match: gi|700208989|gb|KGN64085.1| (hypothetical protein Csa_1G039990 [Cucumis sativus])

HSP 1 Score: 936.0 bits (2418), Expect = 3.3e-269
Identity = 481/613 (78.47%), Postives = 530/613 (86.46%), Query Frame = 1

Query: 1   MADDSTDPFASP-EDRLKALISCPSARTKSGFENESAEGEFQENRPLIDDNSSSICVICF 60
           MADDST+ FAS  EDRLKALISCPSA  K G E E  EG F+E+  LIDDN SSICVICF
Sbjct: 48  MADDSTNSFASSSEDRLKALISCPSASAKFGNEEEFLEGGFEESHLLIDDNPSSICVICF 107

Query: 61  SDDGKAERGKLDSCDHYFCFVCIMEWARIESRCPACKGRFTVVHRIAKDSCRLRERIVNI 120
           S+DGKAERGKLDSCDHYFCFVCIMEWARIESRCPACKGRFTVVHRIAKD CRLRERIVNI
Sbjct: 108 SEDGKAERGKLDSCDHYFCFVCIMEWARIESRCPACKGRFTVVHRIAKDPCRLRERIVNI 167

Query: 121 PMRNQDRSVIENTTNRDPYAETCCTVCKGMEDEGLMLLCDLCDSAAHTYCVGLGANVPEG 180
           PMRNQD+S   +    DPYAETCCTVCKGMEDEGLMLLCDLCDSAAHT+CVGLGANVPEG
Sbjct: 168 PMRNQDQSASGSARIGDPYAETCCTVCKGMEDEGLMLLCDLCDSAAHTFCVGLGANVPEG 227

Query: 181 DWFCHDCTISRAQHADTELNTGYNDQNQTESVRAHISISDIVNESSAQTVGIPRRIALLH 240
           DW+CHDCTI+RAQH DTEL+T +  QNQT +V   I+ISDIV ESSAQTV I RR ALLH
Sbjct: 228 DWYCHDCTIARAQHTDTELDTSFIKQNQTTTVEPRIAISDIVKESSAQTVSITRRGALLH 287

Query: 241 SSHEPPSIVPSWRSRSSVAHKSLPSRGGRAAGTSARTLHRCRNIHSHIRALRENWGGLRR 300
           S+ EPPSIVPS  SR SVA KSLPSRGG+AAGTSARTLHRCRNIHS+IRALR+NW  +R+
Sbjct: 288 SNREPPSIVPS--SRRSVAQKSLPSRGGKAAGTSARTLHRCRNIHSYIRALRDNWDAVRK 347

Query: 301 GSLSFPASSSSTYCSNSSKRDIGAA--VNNQTGEPHFSTAINMQATSGPESNGSSYDVEK 360
           GSL FPASSSSTYC NSSKRD G A  +N QT +PH   AI++QAT+ PESNG+S DVEK
Sbjct: 348 GSLRFPASSSSTYCGNSSKRDTGGAELINKQTNQPH---AISLQATTLPESNGNSCDVEK 407

Query: 361 AWTMMEIAKAKAIREGDQTLKHPASRQMNTKIKDIHTAKGQQFKIQKLKEPATHKKEMGK 420
           AW MMEIAKAK+IREGDQTL+HP SRQ+ TK KD HTAKGQQ+KI+K  EPA HKKEMGK
Sbjct: 408 AWRMMEIAKAKSIREGDQTLRHPVSRQITTKTKDSHTAKGQQYKIKKPDEPAKHKKEMGK 467

Query: 421 QRLPELDKKLCCPVASKKSKASDEFLPTSSSSYGEPSLGNMVQTSRGDFNYENRRKLSNK 480
           QRL ELDK+LC PVASKK+KASDEF PT SSS GE  LG MVQT  GDF+ ENRRK SNK
Sbjct: 468 QRLLELDKQLCRPVASKKNKASDEFFPTHSSSNGEFPLGKMVQTGGGDFHCENRRKPSNK 527

Query: 481 IVDELSSSSTMTQASEKGHAAREAGNDHDPKSEIRTLVKINLKLLSRDKNLGYERYKEVA 540
           IVDE+SSSSTMTQAS+K H+AREAG+DHD KSEIRTLVKINLKLLS+ KNLGYERYKEV 
Sbjct: 528 IVDEVSSSSTMTQASDKHHSAREAGSDHDGKSEIRTLVKINLKLLSQGKNLGYERYKEVT 587

Query: 541 RLATHTIMAACGLEPPPKPSKRFLSCSVCKHTEEEVGQLHRSTLMPESCRECFLVYVKDV 600
           RLATHTIMA CGLEPPPKP+K+++S SVCKHTEEE+ +LHRSTLMP+SCR+CF+ +VKDV
Sbjct: 588 RLATHTIMARCGLEPPPKPTKQYVSSSVCKHTEEEIRKLHRSTLMPDSCRKCFMWFVKDV 647

Query: 601 VNALMLEKLEVGS 611
           VNA+MLEKL VGS
Sbjct: 648 VNAIMLEKLTVGS 655

BLAST of Cp4.1LG20g01060 vs. NCBI nr
Match: gi|823121922|ref|XP_012468856.1| (PREDICTED: uncharacterized protein LOC105786930 isoform X2 [Gossypium raimondii])

HSP 1 Score: 370.9 bits (951), Expect = 4.2e-99
Identity = 261/686 (38.05%), Postives = 353/686 (51.46%), Query Frame = 1

Query: 1   MADDSTDPFASPEDRLKALISCPSARTKSGFENESAEGEFQENRPLIDDNSSSICVICFS 60
           MA DST  F SP    +   S  S        +   E + Q+     DD+   IC IC S
Sbjct: 15  MAGDSTTEFPSPSPNKRLKTSSKSIIEDEPGNHHQPEQQRQQQAADDDDSDPEICGICLS 74

Query: 61  DDGK-AERGKLDSCDHYFCFVCIMEWARIESRCPACKGRFTVVHRIAKDSCRLRERIVNI 120
           D G+ A RGK++SCDH+FCFVCIMEWA++ESRCP CK RFTV+HR  K+     +R+VNI
Sbjct: 75  DGGRRAVRGKINSCDHFFCFVCIMEWAKVESRCPMCKRRFTVIHRPPKEGVFASQRLVNI 134

Query: 121 PMRNQDRSVIENTTN--RDPYAETCCTVCKGMEDEGLMLLCDLCDSAAHTYCVGLGANVP 180
           P R+Q   +  N T+   DPY+E  C++C GM DE L+LLCDLCDSAAHTYCVGLGA VP
Sbjct: 135 PQRDQVYHLSGNATSGLSDPYSEVKCSMCHGMTDESLLLLCDLCDSAAHTYCVGLGATVP 194

Query: 181 EGDWFCHDCTISRAQHADTELNT---------GYNDQNQTESVRAHISISDIVNESSAQT 240
           +GDWFCHDC +SR++H  T+++T          YN Q  +E     +SI DIV +S+   
Sbjct: 195 DGDWFCHDCALSRSEHEKTDVDTETDNHMIAANYNVQLPSEE---DVSIFDIVRDSNITP 254

Query: 241 VGIPRRIALLHSSHEPPSIVPSWRSRSSVAHKSLPSRG--GRAAGTSARTLHRCRNIHSH 300
           +          S+H PPSI+P   S S     S PSR   G++  + ARTL RCRN+ S 
Sbjct: 255 LEGHNASVSSCSNHSPPSIIPGCES-SGADEVSRPSRNTVGKSTESGARTLRRCRNVQSR 314

Query: 301 IRALRENWGGLRRGSLSFPASSSSTYCSNSSKRDIGAAVNNQTGEPHFSTAINMQATSGP 360
           I+ LRENW  L+  SLSF +  + +   +  K  + A  N  + E   S + + Q+TS  
Sbjct: 315 IQVLRENWRALQNRSLSFSSRMAESGGGSCGKGKVAAVCNPGSSESQPSISTSQQSTSRD 374

Query: 361 ES---NGSSYDVEKAWTMMEIAKA----------------------KAIREGDQTLKHPA 420
            S       YD++KAW MM IAK+                       A +E  ++  H  
Sbjct: 375 SSIQPKEDLYDIDKAWKMMNIAKSMQKNCKTTNSLNRNSTKLPCLGSASKEAIRSSSHHI 434

Query: 421 SRQMNTKIKDIHTAKGQQFKIQKLKEPATHKKEMGKQRLPELDKKLCCPVASKKSKASDE 480
                 KI+   T   ++   QK      HK+E  K + PEL+K+    + ++ S    E
Sbjct: 435 -----LKIQQNETRNEERMGKQKQYGYHCHKREKEKCKSPELEKQKRVVINTQPS----E 494

Query: 481 FLPTSSS-SYGEPSLGNMVQTSRGDFNYENRRKLSNKIVDELSSSSTMTQASEKGHAAR- 540
            +P+S S  + +PS    VQ         +      KI       S+ T  +E G  +  
Sbjct: 495 RVPSSHSLHFFQPSSSANVQIENSCSRVNDLGPFV-KIAQSRCRESSSTANTEGGSCSTS 554

Query: 541 -----------------EAG-NDHDP----------------------KSEIRTLVKINL 600
                            EAG N  D                       KSEI+ LVK+NL
Sbjct: 555 LFGSVLRGSMDFLDSNLEAGVNKLDIPEARTRLKKSCSKSKDRKDDNAKSEIQALVKLNL 614

Query: 601 KLLSRDKNLGYERYKEVARLATHTIMAACGLEPPPKPSKRFLSCSVCKHTEEEVGQLHRS 606
           KLLS+DK LG   +KE+ARLATHTI+AA G +   K S       VC H+  ++   H+S
Sbjct: 615 KLLSQDKRLGVVIFKEIARLATHTILAAYGFQ-HSKSSIHSFPSPVCSHS-NDINLQHKS 674

BLAST of Cp4.1LG20g01060 vs. NCBI nr
Match: gi|225455732|ref|XP_002273302.1| (PREDICTED: autophagy-related protein 36 [Vitis vinifera])

HSP 1 Score: 361.3 bits (926), Expect = 3.3e-96
Identity = 246/634 (38.80%), Postives = 337/634 (53.15%), Query Frame = 1

Query: 49  DNSSSICVICFSDDGKAERGKLDSCDHYFCFVCIMEWARIESRCPACKGRFTVVHRIAKD 108
           D  S+ C IC  +D +A RG +DSCDHYFCFVCIMEWA++ESRCP CK RF+ + R  K 
Sbjct: 47  DFDSNCCGICLLEDDRAIRGWVDSCDHYFCFVCIMEWAKVESRCPMCKRRFSTIRRPPKP 106

Query: 109 SCRLRERIVNIPMRNQDRSVIENTTN--RDPYAETCCTVCKGMEDEGLMLLCDLCDSAAH 168
                ERIVN+P+R+Q      N T    DPYAE  C +C G  DE  +L+CDLCDSAAH
Sbjct: 107 GVFPSERIVNVPVRDQAYHHFGNVTTGPSDPYAEVDCGICHGTADESFLLICDLCDSAAH 166

Query: 169 TYCVGLGANVPEGDWFCHDCTISRAQHADTELNTGYNDQN------QTESVRAHISISDI 228
           TYCVGLG  VPEGDWFCHDCT+SRA+HA+ E++  ++D+N      +  S   H+SI DI
Sbjct: 167 TYCVGLGHTVPEGDWFCHDCTVSRAEHANGEIDAVFDDRNNFRNFYRKPSAETHVSICDI 226

Query: 229 VNESSAQTVGIPRRIALLHSSHEPPSIVPSWRSRSSVAHKSLPSRGGRAAGTSARTLHRC 288
           V E   +    P  I   H +   PS   +  S +   + S       ++ + ARTL RC
Sbjct: 227 VAEPYTREDERPPSIGSSHPNR--PSSPIALNSENFAVNNS-------SSMSVARTLLRC 286

Query: 289 RNIHSHIRALRENWGGLRRGSLSFPASSSSTYCSNSSKRDIGAAVNNQTGEPHFSTAINM 348
           RN+++HI+ALRENW  LR GS++F +    +   +S K ++GA+   Q            
Sbjct: 287 RNVNAHIQALRENWNALRSGSMTFSSILGDSGGESSQKYNVGASCPKQ------------ 346

Query: 349 QATSGPESNGSSYDVEKAWTMMEIAKAKAIREG-------DQTLKHPASRQMNTKIKDIH 408
                 ++N  SYD EKAW MM+  KAK+I+          Q  KHP  + +N   K++ 
Sbjct: 347 ------DNNACSYDTEKAWKMMD--KAKSIQRACERTGIIHQVSKHPLGK-VNVP-KEVA 406

Query: 409 TAKGQQFKIQKLKEPATHKKEMG--------KQRLPELDKKLCCPVAS---KKSKASDEF 468
             K            AT+ +++G        K    E D K   P  S   ++ + +   
Sbjct: 407 NVKSSS---------ATNNRQLGNKDFGWHFKHHSLETDNKRYKPQKSENQEQGRVTRTG 466

Query: 469 LP--TSSS-------SYGEPSLGNMVQTSRGDFNYENRRKLSNK-IVDELSS-------- 528
           +P  T SS       S+  PS      + + D ++    + S K +VD  ++        
Sbjct: 467 IPKFTESSLTIHSLVSFDSPSSRKAETSFQVDVHHRTGVRPSQKNLVDSFNAINERDGYG 526

Query: 529 -----------SSTMTQASEKGHAA---------------------REAGNDHDPKSEIR 588
                      S  ++ A+ K  A+                      +A  D D KSEI+
Sbjct: 527 SLIIPDGPVPRSPDLSHANLKFGASSLCKEDLLHRPKGRVEIGSAKNKARRDDDAKSEIQ 586

Query: 589 TLVKINLKLLSRDKNLGYERYKEVARLATHTIMAACGLEPPPKPSKRFLSCSVCKHTEEE 607
           +LVK+NLKL+S+DK LG + +KEVARLATHTI+AACGLE   K   R    S+C H+ E+
Sbjct: 587 SLVKLNLKLISKDKQLGVDTFKEVARLATHTILAACGLE-HSKSCVRSFPTSMCTHS-EQ 638