Cp4.1LG19g01480 (gene) Cucurbita pepo (Zucchini)

Overview
NameCp4.1LG19g01480
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
DescriptionTranscription factor bHLH61-like protein
LocationCp4.1LG19 : 1130589 .. 1131230 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AGAAAGAAAGCAAAAACCTCTGGTGGCGCGTGGCCGGCGAGTGGAGGAGACACGGCGGACGGCGGCTCTGAAATGGGTTCGAGAGAGCGAAAGAAAGCAGCAACATCTTCCTCAATGAATCATGAGAAGCCCACAGAATTTGATGCATCCACAAGCTCTAGTGCTGTACTTTTCTCATAACTCAGCTTTTACCTTTGAATTTCATGCTTTTTTACTCGAGATTTTCAACTATTTTTCCGGAGCGCCACGCTCGGTTGTACCTATCGGCGAATTGACTCTGTCGAACGTGCAGGTGGTGAATGTCGAGAGTTTAGCGAGAGGATTCCTCATAAGCGTATATTTGGAAAAGAATTCGCCCGGATTGTTAATTCGGATTCTAGAAGCCTTCGAAAAATTGGGACTCGAAGTCCTCGATGCTAACGTGTCTTGTTCCGATTGTTTCCAACTCCAAGCAGTCGGGGAAGAGGTTCGAGTTCCATATCATAAGCTCACTAACACGAACATAACCCAACAATTAAAACTTCAAATTCGCTTTTTTTTGTTGTGCAGAATGAAGACACAAAAATCGTAAAGCCCCAAGTGGTGAAAAAAGCAGTAAAGCAAGCAATCAAGGAATGGAGTGAAAGTAATGCGCAGACATGA

mRNA sequence

AGAAAGAAAGCAAAAACCTCTGGTGGCGCGTGGCCGGCGAGTGGAGGAGACACGGCGGACGGCGGCTCTGAAATGGGTTCGAGAGAGCGAAAGAAAGCAGCAACATCTTCCTCAATGAATCATGAGAAGCCCACAGAATTTGATGCATCCACAAGCTCTAGTGCTGTGGTGAATGTCGAGAGTTTAGCGAGAGGATTCCTCATAAGCGTATATTTGGAAAAGAATTCGCCCGGATTGTTAATTCGGATTCTAGAAGCCTTCGAAAAATTGGGACTCGAAGTCCTCGATGCTAACGTGTCTTGTTCCGATTGTTTCCAACTCCAAGCAGTCGGGGAAGAGAATGAAGACACAAAAATCGTAAAGCCCCAAGTGGTGAAAAAAGCAGTAAAGCAAGCAATCAAGGAATGGAGTGAAAGTAATGCGCAGACATGA

Coding sequence (CDS)

AGAAAGAAAGCAAAAACCTCTGGTGGCGCGTGGCCGGCGAGTGGAGGAGACACGGCGGACGGCGGCTCTGAAATGGGTTCGAGAGAGCGAAAGAAAGCAGCAACATCTTCCTCAATGAATCATGAGAAGCCCACAGAATTTGATGCATCCACAAGCTCTAGTGCTGTGGTGAATGTCGAGAGTTTAGCGAGAGGATTCCTCATAAGCGTATATTTGGAAAAGAATTCGCCCGGATTGTTAATTCGGATTCTAGAAGCCTTCGAAAAATTGGGACTCGAAGTCCTCGATGCTAACGTGTCTTGTTCCGATTGTTTCCAACTCCAAGCAGTCGGGGAAGAGAATGAAGACACAAAAATCGTAAAGCCCCAAGTGGTGAAAAAAGCAGTAAAGCAAGCAATCAAGGAATGGAGTGAAAGTAATGCGCAGACATGA

Protein sequence

RKKAKTSGGAWPASGGDTADGGSEMGSRERKKAATSSSMNHEKPTEFDASTSSSAVVNVESLARGFLISVYLEKNSPGLLIRILEAFEKLGLEVLDANVSCSDCFQLQAVGEENEDTKIVKPQVVKKAVKQAIKEWSESNAQT
Homology
[Close]
BLAST of Cp4.1LG19g01480 vs. TrEMBL
Match: A0A0A0L039_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G329560 PE=4 SV=1)

HSP 1 Score: 167.5 bits (423), Expect = 1.1e-38
Identity = 90/120 (75.00%), Postives = 104/120 (86.67%), Query Frame = 1 

Query: 25  MGSRERKKAATSS-SMNHEKPTEFDASTSSSAVVNVESLARGFLISVYLEKNSPGLLIRI 84
           MGSRERKKAA ++ + +H    EF ASTSS+AVVNVES+ RGFLI+V+LE+NSPGLL+RI
Sbjct: 1   MGSRERKKAAAAAPNSSHHNSKEFRASTSSTAVVNVESVERGFLINVFLERNSPGLLVRI 60

Query: 85  LEAFEKLGLEVLDANVSCSDCFQLQAVGEENEDTKIVKPQVVKKAVKQAIKEWSESNAQT 144
           LEAFEKLGL VLDA++SCSDCFQLQA GEENE  KI+K QVVK AVKQAIKEWSES+ QT
Sbjct: 61  LEAFEKLGLGVLDADISCSDCFQLQAFGEENEGRKIIKAQVVKNAVKQAIKEWSESDGQT 120

[Close]
BLAST of Cp4.1LG19g01480 vs. TrEMBL
Match: D7SR22_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_08s0058g00960 PE=4 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 1.8e-20
Identity = 59/99 (59.60%), Postives = 73/99 (73.74%), Query Frame = 1 

Query: 45  TEFDASTSSSAVVNVESLARGFLISVYLEKNSPGLLIRILEAFEKLGLEVLDANVSCSDC 104
           ++F A  +   VV VE+L RGFLI+V+ E+N PGLL+ ILE FE+LGL VLDA VSCSD 
Sbjct: 57  SQFSADQNPLPVVKVETLERGFLINVFSERNCPGLLVSILETFEELGLNVLDARVSCSDS 116

Query: 105 FQLQAVGEENEDTK-IVKPQVVKKAVKQAIKEWSESNAQ 143
           F L+AVG ENE  +  +  QVVK+AV QAIK WSES+ Q
Sbjct: 117 FHLEAVGGENEGQQDSIDAQVVKQAVLQAIKNWSESSEQ 155

[Close]
BLAST of Cp4.1LG19g01480 vs. TrEMBL
Match: A0A0D2S8L1_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_005G006200 PE=4 SV=1)

HSP 1 Score: 104.0 bits (258), Expect = 1.6e-19
Identity = 57/108 (52.78%), Postives = 80/108 (74.07%), Query Frame = 1 

Query: 39  MNHEKPTEFDASTSSS---AVVNVESLARGFLISVYLEKNSPGLLIRILEAFEKLGLEVL 98
           M+ E  T   ASTSS     +V VE+L +GFLI+V+LEKN PGLL+ ILE FE+LGL+VL
Sbjct: 55  MSQETATSSHASTSSQNPLPMVTVETLEKGFLINVFLEKNCPGLLVSILETFEELGLDVL 114

Query: 99  DANVSCSDCFQLQAVGEENE-DTKIVKPQVVKKAVKQAIKEWSESNAQ 143
           +A VSC + F+L+A+G ENE + + +  Q+VK+AV QAI++W E++ Q
Sbjct: 115 EARVSCEESFKLEAIGGENEGNVECIDAQMVKQAVMQAIRKWGENSHQ 162

[Close]
BLAST of Cp4.1LG19g01480 vs. TrEMBL
Match: M5WE19_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa017010mg PE=4 SV=1)

HSP 1 Score: 103.6 bits (257), Expect = 2.0e-19
Identity = 56/90 (62.22%), Postives = 69/90 (76.67%), Query Frame = 1 

Query: 54  SAVVNVESLARGFLISVYLEKNSPGLLIRILEAFEKLGLEVLDANVSCSDCFQLQAVGEE 113
           S  V VE+L RGFLI+V  +KNSPGLL+ ILEAFE+LGL+VLDA VSCSD FQL+AVG E
Sbjct: 63  SPAVTVETLERGFLINVLSDKNSPGLLVSILEAFEELGLDVLDARVSCSDTFQLEAVGGE 122

Query: 114 NE-DTKIVKPQVVKKAVKQAIKEWSESNAQ 143
           N+     +   VVK+AV QAI+ WS+S+ Q
Sbjct: 123 NQGQADSIDAHVVKQAVLQAIQNWSQSSEQ 152

[Close]
BLAST of Cp4.1LG19g01480 vs. TrEMBL
Match: A0A0B0MKH5_GOSAR (Transcription factor bHLH61-like protein OS=Gossypium arboreum GN=F383_25075 PE=4 SV=1)

HSP 1 Score: 103.2 bits (256), Expect = 2.7e-19
Identity = 53/88 (60.23%), Postives = 70/88 (79.55%), Query Frame = 1 

Query: 56  VVNVESLARGFLISVYLEKNSPGLLIRILEAFEKLGLEVLDANVSCSDCFQLQAVGEENE 115
           +V VE+L +GFLI+V+ EKN PGLL+ +LEAFE+LGL +L+A VSC+D F+LQAVG ENE
Sbjct: 68  MVTVETLEKGFLINVFSEKNCPGLLVSLLEAFEELGLNILEARVSCTDSFRLQAVGGENE 127

Query: 116 D-TKIVKPQVVKKAVKQAIKEWSESNAQ 143
           + ++ +  QVVK+AV QAIK WSE   Q
Sbjct: 128 EQSESINAQVVKQAVLQAIKNWSEGTDQ 155

[Close]
BLAST of Cp4.1LG19g01480 vs. TAIR10
Match: AT2G40435.1 (AT2G40435.1 BEST Arabidopsis thaliana protein match is: transcription regulators (TAIR:AT3G56220.1))

HSP 1 Score: 87.4 bits (215), Expect = 7.6e-18
Identity = 47/112 (41.96%), Postives = 71/112 (63.39%), Query Frame = 1 

Query: 29  ERKKAATSSSMNHEKPTEFDASTSSSAVVNVESLARGFLISVYLEKNSPGLLIRILEAFE 88
           ER     ++  +  +PT+       + +V VE+L +GF+I+V+  KN PG+L+ +LEAFE
Sbjct: 48  ERFNQDPTAEQSSSEPTD-----PKTPMVTVETLDKGFMINVFSGKNQPGMLVSVLEAFE 107

Query: 89  KLGLEVLDANVSCSDCFQLQAVGEENEDTKIVKPQVVKKAVKQAIKEWSESN 141
            +GL VL+A  SC+D F L A+G ENED + +  + VK+AV  AI+ W E N
Sbjct: 108 DIGLNVLEARASCTDSFSLHAMGLENEDGENMDAEAVKQAVTDAIRSWGEIN 154

[Close]
BLAST of Cp4.1LG19g01480 vs. TAIR10
Match: AT3G56220.1 (AT3G56220.1 transcription regulators)

HSP 1 Score: 85.1 bits (209), Expect = 3.8e-17
Identity = 43/96 (44.79%), Postives = 65/96 (67.71%), Query Frame = 1 

Query: 47  FDASTSSSAVVNVESLARGFLISVYLEKNSPGLLIRILEAFEKLGLEVLDANVSCSDCFQ 106
           F  S+  + +V VE+L +GF+I V   KN  G+L+ +LE FE LGL+V++A VSC+D F 
Sbjct: 61  FRESSDPNPMVTVETLEKGFMIKVMSRKNEAGMLVCVLETFEDLGLDVVEARVSCTDTFS 120

Query: 107 LQAVG-EENEDTKIVKPQVVKKAVKQAIKEWSESNA 142
           L A+G   N+D   +  + VK+AV +AI+ WS+S+A
Sbjct: 121 LHAIGSSNNDDGDCIDAEAVKQAVAEAIRTWSDSHA 156

[Close]
BLAST of Cp4.1LG19g01480 vs. NCBI nr
Match: gi|659128550|ref|XP_008464258.1| (PREDICTED: uncharacterized protein LOC103502184 [Cucumis melo])

HSP 1 Score: 173.3 bits (438), Expect = 3.0e-40
Identity = 91/118 (77.12%), Postives = 104/118 (88.14%), Query Frame = 1 

Query: 25  MGSRERKKAATSSSMNHEKPTEFDASTSSSAVVNVESLARGFLISVYLEKNSPGLLIRIL 84
           MGSRERKKAA +++ +H    EF ASTSSSAVVNVES+ RGFLI+V+LE+NSPGLL+RIL
Sbjct: 1   MGSRERKKAAAAANSSHHNSKEFRASTSSSAVVNVESVERGFLINVFLERNSPGLLVRIL 60

Query: 85  EAFEKLGLEVLDANVSCSDCFQLQAVGEENEDTKIVKPQVVKKAVKQAIKEWSESNAQ 143
           EAFEKLGL VLDA++SCSDCFQLQAVGEENE  KI+K QVVK AVKQAIKEWSES+ Q
Sbjct: 61  EAFEKLGLGVLDADISCSDCFQLQAVGEENEGRKIIKAQVVKNAVKQAIKEWSESDGQ 118

[Close]
BLAST of Cp4.1LG19g01480 vs. NCBI nr
Match: gi|449464418|ref|XP_004149926.1| (PREDICTED: uncharacterized protein LOC101209807 [Cucumis sativus])

HSP 1 Score: 167.5 bits (423), Expect = 1.6e-38
Identity = 90/120 (75.00%), Postives = 104/120 (86.67%), Query Frame = 1 

Query: 25  MGSRERKKAATSS-SMNHEKPTEFDASTSSSAVVNVESLARGFLISVYLEKNSPGLLIRI 84
           MGSRERKKAA ++ + +H    EF ASTSS+AVVNVES+ RGFLI+V+LE+NSPGLL+RI
Sbjct: 1   MGSRERKKAAAAAPNSSHHNSKEFRASTSSTAVVNVESVERGFLINVFLERNSPGLLVRI 60

Query: 85  LEAFEKLGLEVLDANVSCSDCFQLQAVGEENEDTKIVKPQVVKKAVKQAIKEWSESNAQT 144
           LEAFEKLGL VLDA++SCSDCFQLQA GEENE  KI+K QVVK AVKQAIKEWSES+ QT
Sbjct: 61  LEAFEKLGLGVLDADISCSDCFQLQAFGEENEGRKIIKAQVVKNAVKQAIKEWSESDGQT 120

[Close]
BLAST of Cp4.1LG19g01480 vs. NCBI nr
Match: gi|359481316|ref|XP_002268100.2| (PREDICTED: uncharacterized protein LOC100241403 isoform X2 [Vitis vinifera])

HSP 1 Score: 107.1 bits (266), Expect = 2.6e-20
Identity = 59/99 (59.60%), Postives = 73/99 (73.74%), Query Frame = 1 

Query: 45  TEFDASTSSSAVVNVESLARGFLISVYLEKNSPGLLIRILEAFEKLGLEVLDANVSCSDC 104
           ++F A  +   VV VE+L RGFLI+V+ E+N PGLL+ ILE FE+LGL VLDA VSCSD 
Sbjct: 57  SQFSADQNPLPVVKVETLERGFLINVFSERNCPGLLVSILETFEELGLNVLDARVSCSDS 116

Query: 105 FQLQAVGEENEDTK-IVKPQVVKKAVKQAIKEWSESNAQ 143
           F L+AVG ENE  +  +  QVVK+AV QAIK WSES+ Q
Sbjct: 117 FHLEAVGGENEGQQDSIDAQVVKQAVLQAIKNWSESSEQ 155

[Close]
BLAST of Cp4.1LG19g01480 vs. NCBI nr
Match: gi|645221499|ref|XP_008245174.1| (PREDICTED: transcription factor bHLH61-like isoform X1 [Prunus mume])

HSP 1 Score: 104.0 bits (258), Expect = 2.2e-19
Identity = 57/91 (62.64%), Postives = 70/91 (76.92%), Query Frame = 1 

Query: 53  SSAVVNVESLARGFLISVYLEKNSPGLLIRILEAFEKLGLEVLDANVSCSDCFQLQAVGE 112
           S A V VE+L RGFLI+V  +KNSPGLL+ ILEAFE+LGL+VLDA VSCSD FQL+AVG 
Sbjct: 63  SPAQVTVETLERGFLINVLSDKNSPGLLVSILEAFEELGLDVLDARVSCSDTFQLEAVGG 122

Query: 113 ENE-DTKIVKPQVVKKAVKQAIKEWSESNAQ 143
           EN+     +   VVK+AV QAI+ WS+S+ Q
Sbjct: 123 ENQGQADSIDAHVVKQAVLQAIQNWSQSSEQ 153

[Close]
BLAST of Cp4.1LG19g01480 vs. NCBI nr
Match: gi|823162412|ref|XP_012481156.1| (PREDICTED: transcription factor SCREAM2-like [Gossypium raimondii])

HSP 1 Score: 104.0 bits (258), Expect = 2.2e-19
Identity = 57/108 (52.78%), Postives = 80/108 (74.07%), Query Frame = 1 

Query: 39  MNHEKPTEFDASTSSS---AVVNVESLARGFLISVYLEKNSPGLLIRILEAFEKLGLEVL 98
           M+ E  T   ASTSS     +V VE+L +GFLI+V+LEKN PGLL+ ILE FE+LGL+VL
Sbjct: 55  MSQETATSSHASTSSQNPLPMVTVETLEKGFLINVFLEKNCPGLLVSILETFEELGLDVL 114

Query: 99  DANVSCSDCFQLQAVGEENE-DTKIVKPQVVKKAVKQAIKEWSESNAQ 143
           +A VSC + F+L+A+G ENE + + +  Q+VK+AV QAI++W E++ Q
Sbjct: 115 EARVSCEESFKLEAIGGENEGNVECIDAQMVKQAVMQAIRKWGENSHQ 162

The following BLAST results are available for this feature:
BLAST of Cp4.1LG19g01480 vs. Swiss-Prot
Match NameE-valueIdentityDescription
BLAST of Cp4.1LG19g01480 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0L039_CUCSA1.1e-3875.00Uncharacterized protein OS=Cucumis sativus GN=Csa_4G329560 PE=4 SV=1[more]
D7SR22_VITVI1.8e-2059.60Putative uncharacterized protein OS=Vitis vinifera GN=VIT_08s0058g00960 PE=4 SV=... [more]
A0A0D2S8L1_GOSRA1.6e-1952.78Uncharacterized protein OS=Gossypium raimondii GN=B456_005G006200 PE=4 SV=1[more]
M5WE19_PRUPE2.0e-1962.22Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa017010mg PE=4 SV=1[more]
A0A0B0MKH5_GOSAR2.7e-1960.23Transcription factor bHLH61-like protein OS=Gossypium arboreum GN=F383_25075 PE=... [more]
BLAST of Cp4.1LG19g01480 vs. TAIR10
Match NameE-valueIdentityDescription
AT2G40435.17.6e-1841.96 BEST Arabidopsis thaliana protein match is: transcription regulators... [more]
AT3G56220.13.8e-1744.79 transcription regulators[more]
BLAST of Cp4.1LG19g01480 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|659128550|ref|XP_008464258.1|3.0e-4077.12PREDICTED: uncharacterized protein LOC103502184 [Cucumis melo][more]
gi|449464418|ref|XP_004149926.1|1.6e-3875.00PREDICTED: uncharacterized protein LOC101209807 [Cucumis sativus][more]
gi|359481316|ref|XP_002268100.2|2.6e-2059.60PREDICTED: uncharacterized protein LOC100241403 isoform X2 [Vitis vinifera][more]
gi|645221499|ref|XP_008245174.1|2.2e-1962.64PREDICTED: transcription factor bHLH61-like isoform X1 [Prunus mume][more]
gi|823162412|ref|XP_012481156.1|2.2e-1952.78PREDICTED: transcription factor SCREAM2-like [Gossypium raimondii][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0016597amino acid binding
Vocabulary: Biological Process
TermDefinition
GO:0008152metabolic process
Vocabulary: INTERPRO
TermDefinition
IPR002912ACT_dom
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008152 metabolic process
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0016597 amino acid binding
molecular_function GO:0046983 protein dimerization activity
molecular_function GO:0005488 binding
molecular_function GO:0003674 molecular_function
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG19g01480.1Cp4.1LG19g01480.1mRNA


InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002912ACT domainPROFILEPS51671ACTcoord: 68..143
score: 8
NoneNo IPR availablePANTHERPTHR31945FAMILY NOT NAMEDcoord: 57..140
score: 1.4
NoneNo IPR availablePANTHERPTHR31945:SF21SUBFAMILY NOT NAMEDcoord: 57..140
score: 1.4

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Cp4.1LG19g01480Cucsa.130380Cucumber (Gy14) v1cgycpeB0345
Cp4.1LG19g01480CmaCh03G003070Cucurbita maxima (Rimu)cmacpeB672
Cp4.1LG19g01480CmaCh07G012340Cucurbita maxima (Rimu)cmacpeB880
Cp4.1LG19g01480CmoCh03G003200Cucurbita moschata (Rifu)cmocpeB625
Cp4.1LG19g01480CmoCh07G012790Cucurbita moschata (Rifu)cmocpeB817
Cp4.1LG19g01480Csa4G329560Cucumber (Chinese Long) v2cpecuB486
Cp4.1LG19g01480MELO3C026050Melon (DHL92) v3.5.1cpemeB459
Cp4.1LG19g01480ClCG04G006520Watermelon (Charleston Gray)cpewcgB436
Cp4.1LG19g01480CSPI04G16400Wild cucumber (PI 183967)cpecpiB487
Cp4.1LG19g01480Lsi01G016000Bottle gourd (USVL1VR-Ls)cpelsiB391
Cp4.1LG19g01480MELO3C026050.2Melon (DHL92) v3.6.1cpemedB542
Cp4.1LG19g01480CsaV3_4G027000Cucumber (Chinese Long) v3cpecucB0598
Cp4.1LG19g01480Bhi03G001744Wax gourdcpewgoB0598
Cp4.1LG19g01480CsGy4G015710Cucumber (Gy14) v2cgybcpeB520
Cp4.1LG19g01480Carg19501Silver-seed gourdcarcpeB0893
Cp4.1LG19g01480Carg12665Silver-seed gourdcarcpeB0400
The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
Cp4.1LG19g01480Cp4.1LG10g07670Cucurbita pepo (Zucchini)cpecpeB077
The following block(s) are covering this gene:
GeneOrganismBlock
Cp4.1LG19g01480Cucumber (Chinese Long) v3cpecucB0597
Cp4.1LG19g01480Wax gourdcpewgoB0586
Cp4.1LG19g01480Wild cucumber (PI 183967)cpecpiB486
Cp4.1LG19g01480Cucumber (Chinese Long) v2cpecuB485
Cp4.1LG19g01480Bottle gourd (USVL1VR-Ls)cpelsiB403
Cp4.1LG19g01480Watermelon (Charleston Gray)cpewcgB421
Cp4.1LG19g01480Watermelon (97103) v1cpewmB473
Cp4.1LG19g01480Watermelon (97103) v1cpewmB491
Cp4.1LG19g01480Melon (DHL92) v3.5.1cpemeB441
Cp4.1LG19g01480Cucumber (Gy14) v2cgybcpeB377
Cp4.1LG19g01480Melon (DHL92) v3.6.1cpemedB524