Cp4.1LG19g00460 (gene) Cucurbita pepo (Zucchini)

NameCp4.1LG19g00460
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
DescriptionEf-hand calcium binding protein
LocationCp4.1LG19 : 296260 .. 299051 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCAATTTATTATATCTACTTGTGATCAGCTCCTTCAATTTAGTTTGCTTTTTTGCTTGCGGAGAAAAAAAATGTCGGGTTATCCAAATCAGCCTTCCGGTTACGGTTACGGCTACGGCGGCGCCGGTGGTTACGGAGCTCCACCACCGACGGCTCAGCCTTACGGTTCTTCACCCTACGGCTCGCAACATCCTCCGCCTCACTCTCAGCAGCCGTACGCTCCGGTGGCTGCGCCATACGGTGCACCGTCGGCACCTTACGGGTCAAATCCGGGTGAAAAGCCGCACAAGGACAAGCCTAATCAGTCTCACGGCGGAGTTGGTGGAAGTCATCAGTCTTACGGCGGTCATGGTTATCCACCGCCATCTGCTTATGGAAGCCCTTTCGCCTCACTCTTGCCCTCGGCTTTTCCGCCTGGTACTGATCCGAGCATTGTTACCTGTTTTCAAGTAGCCGATCAGGATGGGAGCGGGTTTATTGATGATAAGGAGTTGCAAGGTGTTCTATCTTCGTATAATCAGAAGTTTAGCATCAGAACTGTTCATCTCCTCATGTACCATTTCACCAACACGAACACAAGGAAGATCGGTAATCTTACTTACTCCCTCTATCGCCTTTTTGTTTGCCCTGGAAATAGGAAATTTCATGAGATTTTTAGTTTCTTAAATCAACAACAGTAATGATGAACAAAAAAAAGAAGAAAAGAAAACCTGTGGGAATCTGGAGAACCCAGGTTAGAGCAAAAAGGCAAACAGTTGGTCAGTGGGTCAGGGGCGACCCTGATCCGGACATCTATCATTAGTTTCAGCTAATTACAATCGAAAAATAGACTGACAAAGATGAAATTCTGTTTATAAATTGAACGGATGGGAACAGGTCATCCGTTCAGTGAAAAACTTTCGAGGTGAATTTCATATAATTGCACTGAATTGAAATATGCCTTTTTAGTGATTAAGATTGATTGTTGTTACTAGTAGTTGATTAGTTCATAAAGTTCATAATTCTTATCTATGAGGTATTCTGCATGATATTCACTAGAAGAACCCGAATACTTTGTATTTCTATACAAGAATCCTCCCAGTGATTTTGCCTGCGATAAGTTGGTGAAGTTATTTTAAGTTTGATTCAAAATGTATTAAGAAAATTTCGAACCTGCTATTTGGACTTATAGTAGTTGTGGTGGTTGCACGAATTTCACTATTTTCTAACTTGTGGTTAAAGTTATTTGACTTTTCATACCTCAAAAGCTTTCGTGTTAATACAGTCTCTGTCATTATACGAGTATTTTTTTGAAGTTATCCAAAGCCATGTCTTTTGAATAGAGTATGCAGTTTAATTGCCATTTCTTTATCTGTTCGAAGCATCTTATTTTATTATTATGATTATGACTTTTTCAATGTAATTGATCTTGAGCAAGCCTTGGTGATTTGAATGGGCGGACAATGCTGCTATAGATTTGATATATTATGCTGGGTTGTTCATCTTTAAATCGTGCCTTTTACTTTTCTATTGACAGTCTTTTGCATTTCTTTTCTGAAAAACAGGACCCAAGGAGTTCATTGCTCTGTTTTATGGTCTTCAGAGCTGGAGGGTAAGCTGAAGAATTGATATTTGTTTTAGCCCAACTTTATCTGTTGGTCTATGAAACTGAGTATTTATTGCATCTTTGTCAGGGTATTTTTGAGAGATTTGATTCAGACAGAAGTGGAAGAATCGATTACAATGAGTTGCGAGAAGCTCTTTTGAGTTTGGGCTTTGCAGTCTCACCGATGGTCTTGGACTTGCTCGTGTCTAAGTTCGACAAAAGTGGCGGGAAAAGCAAGGCCATTGAATATGATAATTTCATAGAGTACGTTTCTCCCCCTTACTCTACATGATACTTGAGGTTTCCATTATCAATATTCTCATTGCCTTTTCCTTTTTGCAAATGCAGGTGCTGTCTTACGGTCAAGGTGAGCTTCTGAATAATTTTATTTTCATATATTTTTCGTATATTACGACATATACTGGTTAGAAGTGATGGCATCATCCGTGTATTTAGTTCTGCATGCTTGCCTACTCGTCTTTAGATCTACTGCAAAACTCAGAACTCGATTTTTTTTCATTCATGTCGTTGCCTATGAATAGCCTGAATATGTTCTACACCATTTTAGTTCCTGTGGCCATCAATTAGTCACATACTATCGGATTTCGTTTCAAGTAAATTGTTCATAATGTTTTCTCACACTGGTATTATGAACTCCTTGATGGTTTTTGTGGCTTCTGCAAACATTTTGATGCCATGGGAGGAAGAAAAGGCTAAAACTGAAGTGCTTTTGATGTTATAAATTACAGGGACTGACTGAGAAATTCAAGGAAAAGGACACCACATATTCGGGTTCTGCAACCTTCAGTTACGAGGCGTTCATGCTGACCGTCCTGCCATTCCTCATCGCCTGATGAGTTTCTCAGCCTTCATTCCAGCATGTGTGGTCAGTGTAAAGCTTGAGTTTTCTTTGGTTTTTCTTTTGAATCTCTGTTGTGTTTGATAGAAACTATATGTATGTAGTCTTATCCTTATCAACGCTCTTTGTTTATGTATAAGTTGGCTATCCTAAGTATTCAATGATAGTTCCATGATTGTCTCAACTATTACCTGTTTTCCTTCCTGGGTTTCCAGTTTTGCATTTGACAGTAAAGTTTAAGTCAATTGTTGTATCAATATAATGATGTGACGTGCAATGAATAACCTAGCATAGTTAGGTATTCAGGTTTCCAATCTTAGCCAAAGAGGGTTGGAAATGGTGTTAAAGT

mRNA sequence

CCAATTTATTATATCTACTTGTGATCAGCTCCTTCAATTTAGTTTGCTTTTTTGCTTGCGGAGAAAAAAAATGTCGGGTTATCCAAATCAGCCTTCCGGTTACGGTTACGGCTACGGCGGCGCCGGTGGTTACGGAGCTCCACCACCGACGGCTCAGCCTTACGGTTCTTCACCCTACGGCTCGCAACATCCTCCGCCTCACTCTCAGCAGCCGTACGCTCCGGTGGCTGCGCCATACGGTGCACCGTCGGCACCTTACGGGTCAAATCCGGGTGAAAAGCCGCACAAGGACAAGCCTAATCAGTCTCACGGCGGAGTTGGTGGAAGTCATCAGTCTTACGGCGGTCATGGTTATCCACCGCCATCTGCTTATGGAAGCCCTTTCGCCTCACTCTTGCCCTCGGCTTTTCCGCCTGGTACTGATCCGAGCATTGTTACCTGTTTTCAAGTAGCCGATCAGGATGGGAGCGGGTTTATTGATGATAAGGAGTTGCAAGGTGTTCTATCTTCGTATAATCAGAAGTTTAGCATCAGAACTGTTCATCTCCTCATGTACCATTTCACCAACACGAACACAAGGAAGATCGGACCCAAGGAGTTCATTGCTCTGTTTTATGGTCTTCAGAGCTGGAGGGGTATTTTTGAGAGATTTGATTCAGACAGAAGTGGAAGAATCGATTACAATGAGTTGCGAGAAGCTCTTTTGAGTTTGGGCTTTGCAGTCTCACCGATGGTCTTGGACTTGCTCGTGTCTAAGTTCGACAAAAGTGGCGGGAAAAGCAAGGCCATTGAATATGATAATTTCATAGAGTGCTGTCTTACGGTCAAGGGACTGACTGAGAAATTCAAGGAAAAGGACACCACATATTCGGGTTCTGCAACCTTCAGTTACGAGGCGTTCATGCTGACCGTCCTGCCATTCCTCATCGCCTGATGAGTTTCTCAGCCTTCATTCCAGCATGTGTGGTCAGTGTAAAGCTTGAGTTTTCTTTGGTTTTTCTTTTGAATCTCTGTTGTGTTTGATAGAAACTATATGTATGTAGTCTTATCCTTATCAACGCTCTTTGTTTATGTATAAGTTGGCTATCCTAAGTATTCAATGATAGTTCCATGATTGTCTCAACTATTACCTGTTTTCCTTCCTGGGTTTCCAGTTTTGCATTTGACAGTAAAGTTTAAGTCAATTGTTGTATCAATATAATGATGTGACGTGCAATGAATAACCTAGCATAGTTAGGTATTCAGGTTTCCAATCTTAGCCAAAGAGGGTTGGAAATGGTGTTAAAGT

Coding sequence (CDS)

ATGTCGGGTTATCCAAATCAGCCTTCCGGTTACGGTTACGGCTACGGCGGCGCCGGTGGTTACGGAGCTCCACCACCGACGGCTCAGCCTTACGGTTCTTCACCCTACGGCTCGCAACATCCTCCGCCTCACTCTCAGCAGCCGTACGCTCCGGTGGCTGCGCCATACGGTGCACCGTCGGCACCTTACGGGTCAAATCCGGGTGAAAAGCCGCACAAGGACAAGCCTAATCAGTCTCACGGCGGAGTTGGTGGAAGTCATCAGTCTTACGGCGGTCATGGTTATCCACCGCCATCTGCTTATGGAAGCCCTTTCGCCTCACTCTTGCCCTCGGCTTTTCCGCCTGGTACTGATCCGAGCATTGTTACCTGTTTTCAAGTAGCCGATCAGGATGGGAGCGGGTTTATTGATGATAAGGAGTTGCAAGGTGTTCTATCTTCGTATAATCAGAAGTTTAGCATCAGAACTGTTCATCTCCTCATGTACCATTTCACCAACACGAACACAAGGAAGATCGGACCCAAGGAGTTCATTGCTCTGTTTTATGGTCTTCAGAGCTGGAGGGGTATTTTTGAGAGATTTGATTCAGACAGAAGTGGAAGAATCGATTACAATGAGTTGCGAGAAGCTCTTTTGAGTTTGGGCTTTGCAGTCTCACCGATGGTCTTGGACTTGCTCGTGTCTAAGTTCGACAAAAGTGGCGGGAAAAGCAAGGCCATTGAATATGATAATTTCATAGAGTGCTGTCTTACGGTCAAGGGACTGACTGAGAAATTCAAGGAAAAGGACACCACATATTCGGGTTCTGCAACCTTCAGTTACGAGGCGTTCATGCTGACCGTCCTGCCATTCCTCATCGCCTGA

Protein sequence

MSGYPNQPSGYGYGYGGAGGYGAPPPTAQPYGSSPYGSQHPPPHSQQPYAPVAAPYGAPSAPYGSNPGEKPHKDKPNQSHGGVGGSHQSYGGHGYPPPSAYGSPFASLLPSAFPPGTDPSIVTCFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTRKIGPKEFIALFYGLQSWRGIFERFDSDRSGRIDYNELREALLSLGFAVSPMVLDLLVSKFDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA
BLAST of Cp4.1LG19g00460 vs. Swiss-Prot
Match: CML49_ARATH (Probable calcium-binding protein CML49 OS=Arabidopsis thaliana GN=CML49 PE=2 SV=1)

HSP 1 Score: 353.2 bits (905), Expect = 2.7e-96
Identity = 210/343 (61.22%), Postives = 238/343 (69.39%), Query Frame = 1

Query: 1   MSGYPNQPSGYGYG---------YGGAGG----YGA----PPPTAQPYGSSPYGSQHPPP 60
           MSGYP    GYGYG         YG  G     YG+    PPP   PYGSS   S +  P
Sbjct: 1   MSGYPPSSQGYGYGGNPPPPQPPYGSTGNNPPPYGSSGSNPPP---PYGSSA-SSPYAVP 60

Query: 61  HSQQPYAPVAAPYGAPSAPYGSNPGE--KPHKDKPN--------------------QSHG 120
           +  QP AP  AP   PSAPY S PG+  KPHK+KP+                      +G
Sbjct: 61  YGAQP-APYGAP---PSAPYASLPGDHNKPHKEKPHGASYGSPSPGGYGAHPSSGPSDYG 120

Query: 121 GVGGSHQSYG----------------GHGYPPP-SAYGSPFASLLPSAFPPGTDPSIVTC 180
           G GG+ Q  G                G+G PPP ++YGSPFASL+PSAFPPGTDP+IV C
Sbjct: 121 GYGGAPQQSGHGGGYGGAPQQSGHGGGYGAPPPQASYGSPFASLVPSAFPPGTDPNIVAC 180

Query: 181 FQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTRKIGPKEFIALFYGL 240
           FQ AD+D SGFIDDKELQG LSSYNQ FSIRTVHLLMY FTN+N RKIGPKEF +LF+ L
Sbjct: 181 FQAADRDNSGFIDDKELQGALSSYNQSFSIRTVHLLMYLFTNSNVRKIGPKEFTSLFFSL 240

Query: 241 QSWRGIFERFDSDRSGRIDYNELREALLSLGFAVSPMVLDLLVSKFDKSGGKSKAIEYDN 288
           Q+WR IFERFD DRSGRID NELR+AL+SLGF+VSP++LDLLVSKFDKSGG+++AIEYDN
Sbjct: 241 QNWRSIFERFDKDRSGRIDTNELRDALMSLGFSVSPVILDLLVSKFDKSGGRNRAIEYDN 300

BLAST of Cp4.1LG19g00460 vs. Swiss-Prot
Match: CML50_ARATH (Probable calcium-binding protein CML50 OS=Arabidopsis thaliana GN=CML50 PE=2 SV=1)

HSP 1 Score: 337.8 bits (865), Expect = 1.2e-91
Identity = 194/288 (67.36%), Postives = 214/288 (74.31%), Query Frame = 1

Query: 4   YPNQPSGYGYGYGGAGGYGAPPPTAQP----YGSSPYGSQHPPPHSQQPYAPVAAPYGAP 63
           Y   P    YG    GGYGAPPP+       YG+ P  SQ P  H     A    P+G  
Sbjct: 86  YNKPPKEKPYG----GGYGAPPPSGSSDYGSYGAGPRPSQ-PSGHGGGYGA--TPPHGV- 145

Query: 64  SAPYGSNPGEKPHKDKPNQSHGGVGGSHQSYGGHGYPPPSAYGSPFASLLPSAFPPGTDP 123
            + YGS  G  P     +  HGG       YGG  YPP ++YGSPFASL+PS F PGTDP
Sbjct: 146 -SDYGSYGGAPPRP--ASSGHGG------GYGG--YPPQASYGSPFASLIPSGFAPGTDP 205

Query: 124 SIVTCFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTRKIGPKEFIA 183
           +IV CFQ ADQDGSGFIDDKELQG LSSY Q+FS+RTVHLLMY FTN+N  KIGPKEF A
Sbjct: 206 NIVACFQAADQDGSGFIDDKELQGALSSYQQRFSMRTVHLLMYLFTNSNAMKIGPKEFTA 265

Query: 184 LFYGLQSWRGIFERFDSDRSGRIDYNELREALLSLGFAVSPMVLDLLVSKFDKSGGKSKA 243
           LFY LQ+WR IFER D DRSGRID NELR+ALLSLGF+VSP+VLDLLVSKFDKSGGK++A
Sbjct: 266 LFYSLQNWRSIFERSDKDRSGRIDVNELRDALLSLGFSVSPVVLDLLVSKFDKSGGKNRA 325

Query: 244 IEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 288
           IEYDNFIECCLTVKGLTEKFKEKDT YSGSATF+YE+FMLTVLPFLIA
Sbjct: 326 IEYDNFIECCLTVKGLTEKFKEKDTAYSGSATFNYESFMLTVLPFLIA 354

BLAST of Cp4.1LG19g00460 vs. Swiss-Prot
Match: CML48_ARATH (Probable calcium-binding protein CML48 OS=Arabidopsis thaliana GN=CML48 PE=2 SV=2)

HSP 1 Score: 145.6 bits (366), Expect = 8.4e-34
Identity = 92/231 (39.83%), Postives = 130/231 (56.28%), Query Frame = 1

Query: 58  APSAPYGSNPGEKPHKDKPNQSHGGVGGSHQSYGGHGYPPPSAYGSPFASLLPSAFPPGT 117
           APSAP      E P      Q  G    S  +Y    Y P   + S         F P T
Sbjct: 8   APSAP------ELPESFVQQQHDGE---SRYTYAYPSYQPTQQFSS-----YSGMFSPET 67

Query: 118 DPSIVTCFQVADQDGSGFIDDKELQGVLS-SYNQKFSIRTVHLLM--YHFTNTNTRKIGP 177
            P IV  F+ AD++ SGF+++ EL+  LS S     S RT+ LL+  Y     +  ++GP
Sbjct: 68  HPEIVRSFESADRNRSGFLEESELRQALSLSGYDGISNRTIRLLLFIYKIPVDSLLRLGP 127

Query: 178 KEFIALFYGLQSWRGIFERFDSDRSGRIDYNELREALLSLGFAVSPMVLDLLVSKFDKSG 237
           KE++ L+  L  WR IF R+D DRSG+++  +LR+A  +LG  +   V  L+VS+FD   
Sbjct: 128 KEYVELWNCLAQWRAIFNRYDRDRSGKMNSTQLRDAFYNLGCVLPTSVHQLIVSQFDDGT 187

Query: 238 GKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFL 286
           GK+  + +D+F+EC + VKGLTEKF+E D  Y+G AT SY+ FML V+PF+
Sbjct: 188 GKTVDLCFDSFLECGMIVKGLTEKFRENDPGYTGYATLSYDVFMLMVIPFI 224

BLAST of Cp4.1LG19g00460 vs. Swiss-Prot
Match: PEF1_XENLA (Peflin OS=Xenopus laevis GN=pef1 PE=1 SV=1)

HSP 1 Score: 136.3 bits (342), Expect = 5.1e-31
Identity = 102/273 (37.36%), Postives = 145/273 (53.11%), Query Frame = 1

Query: 11  YGYGYGGAGGYGAPPPTAQPYGSSPYGSQ--HPPPHSQQPYAPVAAPYG--APSAPYGSN 70
           YG G   A  YG P P A PYGS P G    HP P S  P AP   PYG  AP  PY S 
Sbjct: 31  YGGGVQPAASYGRPAPGA-PYGSPPSGGVYGHPVPGSAAPGAP-GGPYGGQAPGGPY-SV 90

Query: 71  PGEKPHKDKPNQSHGGVGGSHQSYGGHGYPPPSAYGSPFASLLPSAFPPGTDPSIVTCFQ 130
           PG  P+    +Q HG       SYG          G+P  ++     PPG DP   + FQ
Sbjct: 91  PGSTPYG---SQQHG-------SYG---------QGAPAGNI-----PPGVDPEAFSWFQ 150

Query: 131 VADQDGSGFIDDKELQGVLSSYN-QKFSIRTVHLLMYHFTNTNTRKIGPKEFIALFYGLQ 190
             D D SG+I  KEL+  L + N   F+  T  ++M  F  +N+ +I    F AL+  +Q
Sbjct: 151 TVDTDHSGYISLKELKQALVNTNWSSFNDETCTMMMNMFDKSNSGRIDMFGFSALWRFIQ 210

Query: 191 SWRGIFERFDSDRSGRIDYNELREALLSLGFAVSPMVLDLLVSKFDKSGGKSKAIEYDNF 250
            WR +F+++D DRSG I+  EL +AL  +G+ +SP  + +++S++ +   +   ++ D F
Sbjct: 211 QWRNLFQQYDRDRSGSINQGELHQALCQMGYQLSPQFVQIVMSRYAQRSAQ-PGLQLDRF 270

Query: 251 IECCLTVKGLTEKFKEKDTTYSGSATFSYEAFM 279
           I+ C  ++ +TE F+EKDT   G+A  SYE F+
Sbjct: 271 IQICTQLQSMTEAFREKDTGQIGTAKLSYEDFI 275

BLAST of Cp4.1LG19g00460 vs. Swiss-Prot
Match: PEF1_HUMAN (Peflin OS=Homo sapiens GN=PEF1 PE=1 SV=1)

HSP 1 Score: 132.5 bits (332), Expect = 7.4e-30
Identity = 99/282 (35.11%), Postives = 137/282 (48.58%), Query Frame = 1

Query: 3   GYPNQPSGYGYGYGGAGGYGAPPPTAQ---PYGSSPYGSQHPPPHSQQPYAPVAAPYGAP 62
           G PN    YG G    GGYG P P      P G  PYG  HP P           P G P
Sbjct: 28  GPPNSGGQYGSGLPPGGGYGGPAPGGPYGPPAGGGPYG--HPNPGM--------FPSGTP 87

Query: 63  SAPYG-SNPGEKPHKDKPNQSHGGVGGSHQSYGGHGYPPPSAYGSPFASLL-PSAFPPGT 122
             PYG + PG                      G +G PPPS+YG+    L      PP  
Sbjct: 88  GGPYGGAAPG----------------------GPYGQPPPSSYGAQQPGLYGQGGAPPNV 147

Query: 123 DPSIVTCFQVADQDGSGFIDDKELQGVLSSYN-QKFSIRTVHLLMYHFTNTNTRKIGPKE 182
           DP   + FQ  D D SG+I  KEL+  L + N   F+  T  +++  F  T + +I    
Sbjct: 148 DPEAYSWFQSVDSDHSGYISMKELKQALVNCNWSSFNDETCLMMINMFDKTKSGRIDVYG 207

Query: 183 FIALFYGLQSWRGIFERFDSDRSGRIDYNELREALLSLGFAVSPMVLDLLVSKFDKSGGK 242
           F AL+  +Q W+ +F+++D DRSG I Y EL++AL  +G+ +SP    LLVS++      
Sbjct: 208 FSALWKFIQQWKNLFQQYDRDRSGSISYTELQQALSQMGYNLSPQFTQLLVSRYCPRSA- 267

Query: 243 SKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFM 279
           + A++ D FI+ C  ++ LTE F+EKDT   G+   S+E F+
Sbjct: 268 NPAMQLDRFIQVCTQLQVLTEAFREKDTAVQGNIRLSFEDFV 276

BLAST of Cp4.1LG19g00460 vs. TrEMBL
Match: A0A0A0L336_CUCSA (Ef-hand calcium binding protein OS=Cucumis sativus GN=Csa_4G341560 PE=4 SV=1)

HSP 1 Score: 518.1 bits (1333), Expect = 6.9e-144
Identity = 266/292 (91.10%), Postives = 270/292 (92.47%), Query Frame = 1

Query: 1   MSGYPNQPSGYGYGYGGAGGYGAPPPTAQPYGSSPYGSQHPPPHSQQPYAPVAAPYGAPS 60
           MSGYPNQPSGYGYGYG  GGYGAPPPTAQPY SS YG  HP   +QQPYAPVAAPYG PS
Sbjct: 1   MSGYPNQPSGYGYGYGSTGGYGAPPPTAQPYNSSAYG--HPQSQTQQPYAPVAAPYGVPS 60

Query: 61  APYGSNPGEKPHKDKPNQSHGGVGGSHQSYGG-----HGYPPPSAYGSPFASLLPSAFPP 120
           APYGS  GEKP KDKPNQS+GG GG HQSYGG     HGYPPPSAYGSPFASLLPSAFPP
Sbjct: 61  APYGSAQGEKPPKDKPNQSYGGAGGGHQSYGGAASGGHGYPPPSAYGSPFASLLPSAFPP 120

Query: 121 GTDPSIVTCFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTRKIGPK 180
           GTDPSIV CFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTRKIGPK
Sbjct: 121 GTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTRKIGPK 180

Query: 181 EFIALFYGLQSWRGIFERFDSDRSGRIDYNELREALLSLGFAVSPMVLDLLVSKFDKSGG 240
           EFI+LFYGLQSWRGIFERFDSDRSG+ID NELREALLSLGFAVSPMVLDLLVSKFDKSGG
Sbjct: 181 EFISLFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPMVLDLLVSKFDKSGG 240

Query: 241 KSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 288
           KSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA
Sbjct: 241 KSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 290

BLAST of Cp4.1LG19g00460 vs. TrEMBL
Match: A0A061F3Q5_THECC (Calcium-binding EF-hand family protein isoform 1 OS=Theobroma cacao GN=TCM_024538 PE=4 SV=1)

HSP 1 Score: 397.5 bits (1020), Expect = 1.4e-107
Identity = 213/287 (74.22%), Postives = 238/287 (82.93%), Query Frame = 1

Query: 3   GYPNQPSGYGYGYGGAGGYGAPPPTAQPYGSSPYGSQHPPPHSQQPYAP--VAAPYGAPS 62
           GYP  P GYGYG        APP  AQPY ++PY +  PPP   QPY     AAPYGAPS
Sbjct: 4   GYPQHPPGYGYG--------APP--AQPYSAAPYTAGAPPP--PQPYGVPYAAAPYGAPS 63

Query: 63  APYGSNPGEKPHKDKPNQSHGGVGGSHQSYGGHGYPPPSAYGSPFASLLPSAFPPGTDPS 122
           APYG+ PGEKP KDK  Q+HG VGG+  +Y G  YPP   Y SPFA+LLPSAFPPGTDP+
Sbjct: 64  APYGAPPGEKPPKDK-QQAHG-VGGAPPAYSG-SYPPAGGYSSPFAALLPSAFPPGTDPN 123

Query: 123 IVTCFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTRKIGPKEFIAL 182
           +V CFQ+ADQDGSGFIDDKELQ  LSSYNQ FS+RTVHLLMY FTN+N+RKIGPKEF A+
Sbjct: 124 VVACFQMADQDGSGFIDDKELQRALSSYNQSFSLRTVHLLMYLFTNSNSRKIGPKEFTAV 183

Query: 183 FYGLQSWRGIFERFDSDRSGRIDYNELREALLSLGFAVSPMVLDLLVSKFDKSGGKSKAI 242
           FY LQ+WR IFE+FD DRSG+ID NELREALLSLGF+VSP+VLDLLVSKFDK+GGK+KAI
Sbjct: 184 FYSLQNWRAIFEKFDRDRSGKIDANELREALLSLGFSVSPVVLDLLVSKFDKTGGKNKAI 243

Query: 243 EYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 288
           EYDNFIECCLTVKGLTEKF+EKDTTYSG+ATF+YEAFMLTVLPFLIA
Sbjct: 244 EYDNFIECCLTVKGLTEKFREKDTTYSGNATFTYEAFMLTVLPFLIA 275

BLAST of Cp4.1LG19g00460 vs. TrEMBL
Match: E2JF17_VITVI (EF-hand calcium-binding protein OS=Vitis vinifera GN=VIT_00s0252g00130 PE=2 SV=1)

HSP 1 Score: 395.2 bits (1014), Expect = 6.8e-107
Identity = 215/293 (73.38%), Postives = 244/293 (83.28%), Query Frame = 1

Query: 1   MSGYPNQPSGYGYGYGGAGGYGAPPPTAQPYGSSPYGSQHPPPHSQQPYAPVAAPYGAPS 60
           MSGYP+ P G+GYGYG      APPP AQPY ++PYG+  P P   QPYAPVA PYGAPS
Sbjct: 1   MSGYPHAPPGHGYGYG------APPP-AQPYSATPYGA--PAPQGYQPYAPVATPYGAPS 60

Query: 61  APYGSNPGEKPHKDKPNQSHGGVGGSHQ----SYGGHGYPPPSA--YGSPFASLLPSAFP 120
           APY + PGEKP K+  N+ HGG   SHQ    S GG+   PPSA  +GSPFA L+PSAFP
Sbjct: 61  APYAA-PGEKPPKE--NKPHGG--SSHQPPPSSMGGY---PPSAPGFGSPFALLVPSAFP 120

Query: 121 PGTDPSIVTCFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTRKIGP 180
           PGTDP++V CFQ+ADQDGSGFIDDKELQG LSSYNQ+FS+RTVHLLMY FTN+N RKIGP
Sbjct: 121 PGTDPNVVACFQMADQDGSGFIDDKELQGALSSYNQRFSLRTVHLLMYLFTNSNARKIGP 180

Query: 181 KEFIALFYGLQSWRGIFERFDSDRSGRIDYNELREALLSLGFAVSPMVLDLLVSKFDKSG 240
           KEF A+FY LQ+WR IFE FD DRSG+I+  ELREAL+SLGFAVSP+VLDLL+SKFDKSG
Sbjct: 181 KEFTAVFYSLQNWRAIFENFDRDRSGKIEAGELREALMSLGFAVSPVVLDLLLSKFDKSG 240

Query: 241 GKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 288
           G++KAIEYDNFIECCLTVKGLTEKFKEKD+++SGSATFSYE FMLTVLPFLIA
Sbjct: 241 GRNKAIEYDNFIECCLTVKGLTEKFKEKDSSFSGSATFSYENFMLTVLPFLIA 276

BLAST of Cp4.1LG19g00460 vs. TrEMBL
Match: A0A067JQC2_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_21125 PE=4 SV=1)

HSP 1 Score: 392.9 bits (1008), Expect = 3.4e-106
Identity = 211/288 (73.26%), Postives = 232/288 (80.56%), Query Frame = 1

Query: 1   MSGYPNQPSGYGYGYGGAGGYGAPPPTAQPYGSSP-YGSQHPPPHSQQPYAPVAAPYGAP 60
           MSGYP+QP  YGYG      YGA P   QPYG++P YG+Q P     QPY   +APYGAP
Sbjct: 1   MSGYPHQPPSYGYG-APQQPYGASPSPPQPYGAAPPYGAQPPT----QPYGGPSAPYGAP 60

Query: 61  SAPYGSNPGEKPHKDKPNQSHGGVGGSHQSYGGHGYPPPSAYGSPFASLLPSAFPPGTDP 120
              Y   PG+KP KDKP+ S  G            YPP +AYGSPFASL+PSAFPPGTDP
Sbjct: 61  ---YAHPPGDKPPKDKPHSSAPG-----------SYPPSAAYGSPFASLMPSAFPPGTDP 120

Query: 121 SIVTCFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTRKIGPKEFIA 180
           ++V CFQ+ADQDGSGFIDDKELQ  LS+YNQ FS+RTVHLLMYHFTNTNTRKIGPKEF  
Sbjct: 121 NVVACFQLADQDGSGFIDDKELQRALSTYNQSFSLRTVHLLMYHFTNTNTRKIGPKEFTQ 180

Query: 181 LFYGLQSWRGIFERFDSDRSGRIDYNELREALLSLGFAVSPMVLDLLVSKFDKSGGKSKA 240
           +FY LQ+WR IFERFD DRSGRID NELREALLSLGFAVSP+VLDLLVSKFDK+GGK++A
Sbjct: 181 VFYSLQNWRAIFERFDRDRSGRIDPNELREALLSLGFAVSPVVLDLLVSKFDKTGGKARA 240

Query: 241 IEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 288
           IEYDNFIECCLTVKGLTEKFKEKD TYSGSATF+YEAFMLTVLPFLIA
Sbjct: 241 IEYDNFIECCLTVKGLTEKFKEKDATYSGSATFTYEAFMLTVLPFLIA 269

BLAST of Cp4.1LG19g00460 vs. TrEMBL
Match: A0A0S3SU31_PHAAN (Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.08G325300 PE=4 SV=1)

HSP 1 Score: 392.1 bits (1006), Expect = 5.7e-106
Identity = 210/302 (69.54%), Postives = 235/302 (77.81%), Query Frame = 1

Query: 1   MSGYPNQPSGYGYGYGGAGGYGAPPPT---------------AQPYGSSPYGSQHPPPHS 60
           MSGYPNQP+GYGYG      YGAPPP+               +Q YG+ P G  +  P S
Sbjct: 1   MSGYPNQPTGYGYGAPPPSQYGAPPPSQYGAPPPSQYGAPPPSQSYGAPPSGQSYGAPSS 60

Query: 61  QQPYAPVAAPYGAPSAPYGSNPGEKPHKDKPNQSHGGVGGSHQSYGGHGYPPPSAYGSPF 120
            Q Y+  A+PY  PSAPY + P  KP K+          GSH    G GYPP ++YGSPF
Sbjct: 61  GQSYS--ASPYSQPSAPYAA-PYSKPPKE----------GSHSQGSGGGYPPAASYGSPF 120

Query: 121 ASLLPSAFPPGTDPSIVTCFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFT 180
           ASL+PSAFPPGTDPSIV CFQ+ADQDGSGFIDDKELQ  LSSYNQ FS+RTVHLLMYHFT
Sbjct: 121 ASLVPSAFPPGTDPSIVACFQIADQDGSGFIDDKELQRALSSYNQSFSLRTVHLLMYHFT 180

Query: 181 NTNTRKIGPKEFIALFYGLQSWRGIFERFDSDRSGRIDYNELREALLSLGFAVSPMVLDL 240
           N+N +KIGPKEF +LFY LQ+WR IFERFD DRSG+ID NELR+ALLSLG+AVSP+VLDL
Sbjct: 181 NSNVKKIGPKEFTSLFYSLQNWRSIFERFDKDRSGKIDSNELRDALLSLGYAVSPVVLDL 240

Query: 241 LVSKFDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFL 288
           LVSKFDK+GGKSKAIEYDNFIECCLTVKGLT+KFKEKDT Y+GSATFSYEAFMLTVLPFL
Sbjct: 241 LVSKFDKTGGKSKAIEYDNFIECCLTVKGLTDKFKEKDTAYTGSATFSYEAFMLTVLPFL 289

BLAST of Cp4.1LG19g00460 vs. TAIR10
Match: AT3G10300.3 (AT3G10300.3 Calcium-binding EF-hand family protein)

HSP 1 Score: 353.2 bits (905), Expect = 1.5e-97
Identity = 210/343 (61.22%), Postives = 238/343 (69.39%), Query Frame = 1

Query: 1   MSGYPNQPSGYGYG---------YGGAGG----YGA----PPPTAQPYGSSPYGSQHPPP 60
           MSGYP    GYGYG         YG  G     YG+    PPP   PYGSS   S +  P
Sbjct: 1   MSGYPPSSQGYGYGGNPPPPQPPYGSTGNNPPPYGSSGSNPPP---PYGSSA-SSPYAVP 60

Query: 61  HSQQPYAPVAAPYGAPSAPYGSNPGE--KPHKDKPN--------------------QSHG 120
           +  QP AP  AP   PSAPY S PG+  KPHK+KP+                      +G
Sbjct: 61  YGAQP-APYGAP---PSAPYASLPGDHNKPHKEKPHGASYGSPSPGGYGAHPSSGPSDYG 120

Query: 121 GVGGSHQSYG----------------GHGYPPP-SAYGSPFASLLPSAFPPGTDPSIVTC 180
           G GG+ Q  G                G+G PPP ++YGSPFASL+PSAFPPGTDP+IV C
Sbjct: 121 GYGGAPQQSGHGGGYGGAPQQSGHGGGYGAPPPQASYGSPFASLVPSAFPPGTDPNIVAC 180

Query: 181 FQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTRKIGPKEFIALFYGL 240
           FQ AD+D SGFIDDKELQG LSSYNQ FSIRTVHLLMY FTN+N RKIGPKEF +LF+ L
Sbjct: 181 FQAADRDNSGFIDDKELQGALSSYNQSFSIRTVHLLMYLFTNSNVRKIGPKEFTSLFFSL 240

Query: 241 QSWRGIFERFDSDRSGRIDYNELREALLSLGFAVSPMVLDLLVSKFDKSGGKSKAIEYDN 288
           Q+WR IFERFD DRSGRID NELR+AL+SLGF+VSP++LDLLVSKFDKSGG+++AIEYDN
Sbjct: 241 QNWRSIFERFDKDRSGRIDTNELRDALMSLGFSVSPVILDLLVSKFDKSGGRNRAIEYDN 300

BLAST of Cp4.1LG19g00460 vs. TAIR10
Match: AT5G04170.1 (AT5G04170.1 Calcium-binding EF-hand family protein)

HSP 1 Score: 337.8 bits (865), Expect = 6.5e-93
Identity = 194/288 (67.36%), Postives = 214/288 (74.31%), Query Frame = 1

Query: 4   YPNQPSGYGYGYGGAGGYGAPPPTAQP----YGSSPYGSQHPPPHSQQPYAPVAAPYGAP 63
           Y   P    YG    GGYGAPPP+       YG+ P  SQ P  H     A    P+G  
Sbjct: 86  YNKPPKEKPYG----GGYGAPPPSGSSDYGSYGAGPRPSQ-PSGHGGGYGA--TPPHGV- 145

Query: 64  SAPYGSNPGEKPHKDKPNQSHGGVGGSHQSYGGHGYPPPSAYGSPFASLLPSAFPPGTDP 123
            + YGS  G  P     +  HGG       YGG  YPP ++YGSPFASL+PS F PGTDP
Sbjct: 146 -SDYGSYGGAPPRP--ASSGHGG------GYGG--YPPQASYGSPFASLIPSGFAPGTDP 205

Query: 124 SIVTCFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTRKIGPKEFIA 183
           +IV CFQ ADQDGSGFIDDKELQG LSSY Q+FS+RTVHLLMY FTN+N  KIGPKEF A
Sbjct: 206 NIVACFQAADQDGSGFIDDKELQGALSSYQQRFSMRTVHLLMYLFTNSNAMKIGPKEFTA 265

Query: 184 LFYGLQSWRGIFERFDSDRSGRIDYNELREALLSLGFAVSPMVLDLLVSKFDKSGGKSKA 243
           LFY LQ+WR IFER D DRSGRID NELR+ALLSLGF+VSP+VLDLLVSKFDKSGGK++A
Sbjct: 266 LFYSLQNWRSIFERSDKDRSGRIDVNELRDALLSLGFSVSPVVLDLLVSKFDKSGGKNRA 325

Query: 244 IEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 288
           IEYDNFIECCLTVKGLTEKFKEKDT YSGSATF+YE+FMLTVLPFLIA
Sbjct: 326 IEYDNFIECCLTVKGLTEKFKEKDTAYSGSATFNYESFMLTVLPFLIA 354

BLAST of Cp4.1LG19g00460 vs. TAIR10
Match: AT2G27480.1 (AT2G27480.1 Calcium-binding EF-hand family protein)

HSP 1 Score: 145.6 bits (366), Expect = 4.7e-35
Identity = 92/231 (39.83%), Postives = 130/231 (56.28%), Query Frame = 1

Query: 58  APSAPYGSNPGEKPHKDKPNQSHGGVGGSHQSYGGHGYPPPSAYGSPFASLLPSAFPPGT 117
           APSAP      E P      Q  G    S  +Y    Y P   + S         F P T
Sbjct: 8   APSAP------ELPESFVQQQHDGE---SRYTYAYPSYQPTQQFSS-----YSGMFSPET 67

Query: 118 DPSIVTCFQVADQDGSGFIDDKELQGVLS-SYNQKFSIRTVHLLM--YHFTNTNTRKIGP 177
            P IV  F+ AD++ SGF+++ EL+  LS S     S RT+ LL+  Y     +  ++GP
Sbjct: 68  HPEIVRSFESADRNRSGFLEESELRQALSLSGYDGISNRTIRLLLFIYKIPVDSLLRLGP 127

Query: 178 KEFIALFYGLQSWRGIFERFDSDRSGRIDYNELREALLSLGFAVSPMVLDLLVSKFDKSG 237
           KE++ L+  L  WR IF R+D DRSG+++  +LR+A  +LG  +   V  L+VS+FD   
Sbjct: 128 KEYVELWNCLAQWRAIFNRYDRDRSGKMNSTQLRDAFYNLGCVLPTSVHQLIVSQFDDGT 187

Query: 238 GKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFL 286
           GK+  + +D+F+EC + VKGLTEKF+E D  Y+G AT SY+ FML V+PF+
Sbjct: 188 GKTVDLCFDSFLECGMIVKGLTEKFRENDPGYTGYATLSYDVFMLMVIPFI 224

BLAST of Cp4.1LG19g00460 vs. TAIR10
Match: AT4G37010.2 (AT4G37010.2 centrin 2)

HSP 1 Score: 52.0 bits (123), Expect = 7.1e-07
Identity = 32/85 (37.65%), Postives = 50/85 (58.82%), Query Frame = 1

Query: 188 RGIFERFDSDRSGRIDYNELREALLSLGFAVSPMVLDLLVSKFDKSGGKSKAIEYDNFIE 247
           R IF+ FD D SG ID +EL  A+ SLGF ++   ++ L+++ DK+  +S AI++D F+ 
Sbjct: 33  REIFDLFDIDGSGSIDASELNVAMRSLGFEMNNQQINELMAEVDKN--QSGAIDFDEFVH 92

Query: 248 CCLTVKGLTEKFKEKDTTYSGSATF 273
                  +T KF E+D+    S  F
Sbjct: 93  M------MTTKFGERDSIDELSKAF 109

BLAST of Cp4.1LG19g00460 vs. TAIR10
Match: AT3G50360.1 (AT3G50360.1 centrin2)

HSP 1 Score: 52.0 bits (123), Expect = 7.1e-07
Identity = 34/100 (34.00%), Postives = 52/100 (52.00%), Query Frame = 1

Query: 170 RKIGPKEFIALFYGL-----QSWRGIFERFDSDRSGRIDYNELREALLSLGFAVSPMVLD 229
           R +  KE     +GL     Q  +  FE FD+D SG ID  EL  A+ +LGF ++   ++
Sbjct: 6   RTVSRKEKPRRHHGLTTQKKQEIKEAFELFDTDGSGTIDAKELNVAMRALGFEMTEEQIN 65

Query: 230 LLVSKFDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDT 265
            +++  DK G  S AI++D F+        +T K  E+DT
Sbjct: 66  KMIADVDKDG--SGAIDFDEFVHM------MTAKIGERDT 97

BLAST of Cp4.1LG19g00460 vs. NCBI nr
Match: gi|449449801|ref|XP_004142653.1| (PREDICTED: probable calcium-binding protein CML49 [Cucumis sativus])

HSP 1 Score: 518.1 bits (1333), Expect = 1.0e-143
Identity = 266/292 (91.10%), Postives = 270/292 (92.47%), Query Frame = 1

Query: 1   MSGYPNQPSGYGYGYGGAGGYGAPPPTAQPYGSSPYGSQHPPPHSQQPYAPVAAPYGAPS 60
           MSGYPNQPSGYGYGYG  GGYGAPPPTAQPY SS YG  HP   +QQPYAPVAAPYG PS
Sbjct: 1   MSGYPNQPSGYGYGYGSTGGYGAPPPTAQPYNSSAYG--HPQSQTQQPYAPVAAPYGVPS 60

Query: 61  APYGSNPGEKPHKDKPNQSHGGVGGSHQSYGG-----HGYPPPSAYGSPFASLLPSAFPP 120
           APYGS  GEKP KDKPNQS+GG GG HQSYGG     HGYPPPSAYGSPFASLLPSAFPP
Sbjct: 61  APYGSAQGEKPPKDKPNQSYGGAGGGHQSYGGAASGGHGYPPPSAYGSPFASLLPSAFPP 120

Query: 121 GTDPSIVTCFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTRKIGPK 180
           GTDPSIV CFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTRKIGPK
Sbjct: 121 GTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTRKIGPK 180

Query: 181 EFIALFYGLQSWRGIFERFDSDRSGRIDYNELREALLSLGFAVSPMVLDLLVSKFDKSGG 240
           EFI+LFYGLQSWRGIFERFDSDRSG+ID NELREALLSLGFAVSPMVLDLLVSKFDKSGG
Sbjct: 181 EFISLFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPMVLDLLVSKFDKSGG 240

Query: 241 KSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 288
           KSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA
Sbjct: 241 KSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 290

BLAST of Cp4.1LG19g00460 vs. NCBI nr
Match: gi|659129266|ref|XP_008464601.1| (PREDICTED: probable calcium-binding protein CML49 [Cucumis melo])

HSP 1 Score: 506.9 bits (1304), Expect = 2.3e-140
Identity = 263/294 (89.46%), Postives = 270/294 (91.84%), Query Frame = 1

Query: 1   MSGYPNQPSGYGYGYG--GAGGYGAPPPTAQPYGSSPYGSQHPPPHSQQPYAPVAAPYGA 60
           MSGYPNQPSGYGYGYG  G GGYGAPPPTAQPY SS YG  HP   +QQPYAPVAAPYG 
Sbjct: 1   MSGYPNQPSGYGYGYGYGGTGGYGAPPPTAQPYNSSAYG--HPQSQTQQPYAPVAAPYGG 60

Query: 61  PSAPYGSNPGEKPHKDKPNQSHGGVGGSHQSYGG-----HGYPPPSAYGSPFASLLPSAF 120
           PSAPY +  GEKP KDKPNQS+GG GG HQSYGG     HGYPPPSAYG+PFASLLPSAF
Sbjct: 61  PSAPYSAQ-GEKPPKDKPNQSYGGAGGGHQSYGGAAGGGHGYPPPSAYGNPFASLLPSAF 120

Query: 121 PPGTDPSIVTCFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTRKIG 180
           PPGTDPSIV CFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTRKIG
Sbjct: 121 PPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTRKIG 180

Query: 181 PKEFIALFYGLQSWRGIFERFDSDRSGRIDYNELREALLSLGFAVSPMVLDLLVSKFDKS 240
           PKEFI+LFYGLQSWRGIFERFDSDRSG+ID NELR+ALLSLGFAVSPMVLDLLVSKFDKS
Sbjct: 181 PKEFISLFYGLQSWRGIFERFDSDRSGKIDSNELRDALLSLGFAVSPMVLDLLVSKFDKS 240

Query: 241 GGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 288
           GGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA
Sbjct: 241 GGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 291

BLAST of Cp4.1LG19g00460 vs. NCBI nr
Match: gi|590635473|ref|XP_007028641.1| (EF hand family protein, expressed isoform 1 [Theobroma cacao])

HSP 1 Score: 397.5 bits (1020), Expect = 2.0e-107
Identity = 213/287 (74.22%), Postives = 238/287 (82.93%), Query Frame = 1

Query: 3   GYPNQPSGYGYGYGGAGGYGAPPPTAQPYGSSPYGSQHPPPHSQQPYAP--VAAPYGAPS 62
           GYP  P GYGYG        APP  AQPY ++PY +  PPP   QPY     AAPYGAPS
Sbjct: 4   GYPQHPPGYGYG--------APP--AQPYSAAPYTAGAPPP--PQPYGVPYAAAPYGAPS 63

Query: 63  APYGSNPGEKPHKDKPNQSHGGVGGSHQSYGGHGYPPPSAYGSPFASLLPSAFPPGTDPS 122
           APYG+ PGEKP KDK  Q+HG VGG+  +Y G  YPP   Y SPFA+LLPSAFPPGTDP+
Sbjct: 64  APYGAPPGEKPPKDK-QQAHG-VGGAPPAYSG-SYPPAGGYSSPFAALLPSAFPPGTDPN 123

Query: 123 IVTCFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTRKIGPKEFIAL 182
           +V CFQ+ADQDGSGFIDDKELQ  LSSYNQ FS+RTVHLLMY FTN+N+RKIGPKEF A+
Sbjct: 124 VVACFQMADQDGSGFIDDKELQRALSSYNQSFSLRTVHLLMYLFTNSNSRKIGPKEFTAV 183

Query: 183 FYGLQSWRGIFERFDSDRSGRIDYNELREALLSLGFAVSPMVLDLLVSKFDKSGGKSKAI 242
           FY LQ+WR IFE+FD DRSG+ID NELREALLSLGF+VSP+VLDLLVSKFDK+GGK+KAI
Sbjct: 184 FYSLQNWRAIFEKFDRDRSGKIDANELREALLSLGFSVSPVVLDLLVSKFDKTGGKNKAI 243

Query: 243 EYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 288
           EYDNFIECCLTVKGLTEKF+EKDTTYSG+ATF+YEAFMLTVLPFLIA
Sbjct: 244 EYDNFIECCLTVKGLTEKFREKDTTYSGNATFTYEAFMLTVLPFLIA 275

BLAST of Cp4.1LG19g00460 vs. NCBI nr
Match: gi|526117928|ref|NP_001267893.1| (uncharacterized protein LOC100248601 [Vitis vinifera])

HSP 1 Score: 395.2 bits (1014), Expect = 9.7e-107
Identity = 215/293 (73.38%), Postives = 244/293 (83.28%), Query Frame = 1

Query: 1   MSGYPNQPSGYGYGYGGAGGYGAPPPTAQPYGSSPYGSQHPPPHSQQPYAPVAAPYGAPS 60
           MSGYP+ P G+GYGYG      APPP AQPY ++PYG+  P P   QPYAPVA PYGAPS
Sbjct: 1   MSGYPHAPPGHGYGYG------APPP-AQPYSATPYGA--PAPQGYQPYAPVATPYGAPS 60

Query: 61  APYGSNPGEKPHKDKPNQSHGGVGGSHQ----SYGGHGYPPPSA--YGSPFASLLPSAFP 120
           APY + PGEKP K+  N+ HGG   SHQ    S GG+   PPSA  +GSPFA L+PSAFP
Sbjct: 61  APYAA-PGEKPPKE--NKPHGG--SSHQPPPSSMGGY---PPSAPGFGSPFALLVPSAFP 120

Query: 121 PGTDPSIVTCFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTRKIGP 180
           PGTDP++V CFQ+ADQDGSGFIDDKELQG LSSYNQ+FS+RTVHLLMY FTN+N RKIGP
Sbjct: 121 PGTDPNVVACFQMADQDGSGFIDDKELQGALSSYNQRFSLRTVHLLMYLFTNSNARKIGP 180

Query: 181 KEFIALFYGLQSWRGIFERFDSDRSGRIDYNELREALLSLGFAVSPMVLDLLVSKFDKSG 240
           KEF A+FY LQ+WR IFE FD DRSG+I+  ELREAL+SLGFAVSP+VLDLL+SKFDKSG
Sbjct: 181 KEFTAVFYSLQNWRAIFENFDRDRSGKIEAGELREALMSLGFAVSPVVLDLLLSKFDKSG 240

Query: 241 GKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 288
           G++KAIEYDNFIECCLTVKGLTEKFKEKD+++SGSATFSYE FMLTVLPFLIA
Sbjct: 241 GRNKAIEYDNFIECCLTVKGLTEKFKEKDSSFSGSATFSYENFMLTVLPFLIA 276

BLAST of Cp4.1LG19g00460 vs. NCBI nr
Match: gi|802730522|ref|XP_012086224.1| (PREDICTED: probable calcium-binding protein CML49 [Jatropha curcas])

HSP 1 Score: 392.9 bits (1008), Expect = 4.8e-106
Identity = 211/288 (73.26%), Postives = 232/288 (80.56%), Query Frame = 1

Query: 1   MSGYPNQPSGYGYGYGGAGGYGAPPPTAQPYGSSP-YGSQHPPPHSQQPYAPVAAPYGAP 60
           MSGYP+QP  YGYG      YGA P   QPYG++P YG+Q P     QPY   +APYGAP
Sbjct: 1   MSGYPHQPPSYGYG-APQQPYGASPSPPQPYGAAPPYGAQPPT----QPYGGPSAPYGAP 60

Query: 61  SAPYGSNPGEKPHKDKPNQSHGGVGGSHQSYGGHGYPPPSAYGSPFASLLPSAFPPGTDP 120
              Y   PG+KP KDKP+ S  G            YPP +AYGSPFASL+PSAFPPGTDP
Sbjct: 61  ---YAHPPGDKPPKDKPHSSAPG-----------SYPPSAAYGSPFASLMPSAFPPGTDP 120

Query: 121 SIVTCFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTRKIGPKEFIA 180
           ++V CFQ+ADQDGSGFIDDKELQ  LS+YNQ FS+RTVHLLMYHFTNTNTRKIGPKEF  
Sbjct: 121 NVVACFQLADQDGSGFIDDKELQRALSTYNQSFSLRTVHLLMYHFTNTNTRKIGPKEFTQ 180

Query: 181 LFYGLQSWRGIFERFDSDRSGRIDYNELREALLSLGFAVSPMVLDLLVSKFDKSGGKSKA 240
           +FY LQ+WR IFERFD DRSGRID NELREALLSLGFAVSP+VLDLLVSKFDK+GGK++A
Sbjct: 181 VFYSLQNWRAIFERFDRDRSGRIDPNELREALLSLGFAVSPVVLDLLVSKFDKTGGKARA 240

Query: 241 IEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 288
           IEYDNFIECCLTVKGLTEKFKEKD TYSGSATF+YEAFMLTVLPFLIA
Sbjct: 241 IEYDNFIECCLTVKGLTEKFKEKDATYSGSATFTYEAFMLTVLPFLIA 269

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
CML49_ARATH2.7e-9661.22Probable calcium-binding protein CML49 OS=Arabidopsis thaliana GN=CML49 PE=2 SV=... [more]
CML50_ARATH1.2e-9167.36Probable calcium-binding protein CML50 OS=Arabidopsis thaliana GN=CML50 PE=2 SV=... [more]
CML48_ARATH8.4e-3439.83Probable calcium-binding protein CML48 OS=Arabidopsis thaliana GN=CML48 PE=2 SV=... [more]
PEF1_XENLA5.1e-3137.36Peflin OS=Xenopus laevis GN=pef1 PE=1 SV=1[more]
PEF1_HUMAN7.4e-3035.11Peflin OS=Homo sapiens GN=PEF1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0L336_CUCSA6.9e-14491.10Ef-hand calcium binding protein OS=Cucumis sativus GN=Csa_4G341560 PE=4 SV=1[more]
A0A061F3Q5_THECC1.4e-10774.22Calcium-binding EF-hand family protein isoform 1 OS=Theobroma cacao GN=TCM_02453... [more]
E2JF17_VITVI6.8e-10773.38EF-hand calcium-binding protein OS=Vitis vinifera GN=VIT_00s0252g00130 PE=2 SV=1[more]
A0A067JQC2_JATCU3.4e-10673.26Uncharacterized protein OS=Jatropha curcas GN=JCGZ_21125 PE=4 SV=1[more]
A0A0S3SU31_PHAAN5.7e-10669.54Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.08G325300 PE=... [more]
Match NameE-valueIdentityDescription
AT3G10300.31.5e-9761.22 Calcium-binding EF-hand family protein[more]
AT5G04170.16.5e-9367.36 Calcium-binding EF-hand family protein[more]
AT2G27480.14.7e-3539.83 Calcium-binding EF-hand family protein[more]
AT4G37010.27.1e-0737.65 centrin 2[more]
AT3G50360.17.1e-0734.00 centrin2[more]
Match NameE-valueIdentityDescription
gi|449449801|ref|XP_004142653.1|1.0e-14391.10PREDICTED: probable calcium-binding protein CML49 [Cucumis sativus][more]
gi|659129266|ref|XP_008464601.1|2.3e-14089.46PREDICTED: probable calcium-binding protein CML49 [Cucumis melo][more]
gi|590635473|ref|XP_007028641.1|2.0e-10774.22EF hand family protein, expressed isoform 1 [Theobroma cacao][more]
gi|526117928|ref|NP_001267893.1|9.7e-10773.38uncharacterized protein LOC100248601 [Vitis vinifera][more]
gi|802730522|ref|XP_012086224.1|4.8e-10673.26PREDICTED: probable calcium-binding protein CML49 [Jatropha curcas][more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0005509calcium ion binding
Vocabulary: INTERPRO
TermDefinition
IPR018247EF_Hand_1_Ca_BS
IPR011992EF-hand-dom_pair
IPR002048EF_hand_dom
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
biological_process GO:0051640 organelle localization
cellular_component GO:0005575 cellular_component
molecular_function GO:0005509 calcium ion binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG19g00460.1Cp4.1LG19g00460.1mRNA


Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainPFAMPF00036EF-hand_1coord: 188..212
score: 3.
IPR002048EF-hand domainPFAMPF13202EF-hand_5coord: 122..144
score: 1.
IPR002048EF-hand domainSMARTSM00054efh_1coord: 120..148
score: 0.51coord: 186..214
score: 0.
IPR002048EF-hand domainPROFILEPS50222EF_HAND_2coord: 182..217
score: 13.202coord: 116..151
score: 10
IPR011992EF-hand domain pairGENE3DG3DSA:1.10.238.10coord: 123..282
score: 2.4
IPR011992EF-hand domain pairunknownSSF47473EF-handcoord: 115..282
score: 2.85
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 195..207
score: -coord: 129..141
scor
NoneNo IPR availablePANTHERPTHR10183CALPAINcoord: 94..287
score: 7.0