CmaCh03G000450 (gene) Cucurbita maxima (Rimu)

NameCmaCh03G000450
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionEf-hand calcium binding protein
LocationCma_Chr03 : 398195 .. 401748 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexonthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCAGGTTACCGAAATCAACCTTCCGGTTACGGTTATAGTTACGGCGGTGGTGGCGGTTACGGAGCTCCACCGCCGACGTCTCAGCCGTACAGTTCTCCACCCTACGGCTCGCAACATCCTCCGTCACAGTCTCAGCAGCCGTACGCTCCGGTGGCCGCGCAATATGGCGCACCGTCGGCACCGTATGGATCATCTCCGGGCGAAAAACCGCCCAAGGACAAGCCTAATCAGTCTTACGGCGGATCAGCCAGCGGTGGTCATGGTTATCCACCGCCTTCTGCGTATGGAAGCCCATTTGCCTCACTCATGCCCTCCGCCTTTCCGCCTGGGACTGATCCGAGCATCGTTGCGTGTTTTCAAGTAGCCGATCGGGATAGGAGTGGGTTTATTGATGATAAGGAGTTGCAAGGTGTTCTGTCATCGTATAATCAGAAGTTTAGCATGAGAACTGTTCACCTCCTGATGTTTCATTTCACCAACACAAGCACCAGGAAGATCGGTAATCTCTCTGCTCCCTCTATCGCCTTTTTGTTTGCCCTGGCAACAATAAAATTTCATGTGGCTATGAGAAAAAGGANGAGAGAACCTGTGGGAATCTGGAGGGCCCAGATAGAGAAAAAAGACAAACAGTTTGGCCAGTGGGGCAGGGGAGGTCTTACTATTAGTTTAAGCTAATTTGAACATAATCTGACAGAGATCAATGTCTATTTATAAATTGAACGCTTCATTGTTAGAACTTTCGAGGTGAATTTCATATAATTGCATAGAATTGTTATCTTCCTTTTTAGTGATTTTTAGTGATTATGATGGTTTCTTATTTCTGTTAGTTGATCTGTTTATGTTAGGAATCACGACTCTCTATAATGATATGATATTATCCACTTTGAGCATAAATTCTCGTTTTTACTTTTGGTTTCCTCAAAAGGCCTCGTACTTTTGTTATAGTGTTCTTACTTATAAACCCACGATCTTCCTCCTAGTTAGCCAATGTGAGACTACTCCTGCCAATAATTCTCAACAATCCTCTCCTCGAACAAAGTACATCATAGAGCCTCCCGGAGCCCTCGGATAGCCTCCCCTTAATCGAGGCTCGACTCCTTTCTTTGGCGCCCCTTTTGTTCGACACTTGAGTCATTTTTTACTATACTTTCGAGGCTCACAACTTCTTTGTTTGACATTTGAGGATTCTATTGACATGACTAAGTTAAGAGCATGACTCTGATACCATGTTAGGAATCACAACACTCCACAATGTATGATATTGTCCACTTTGAGCATAAGGTCTCATGACCTTGCTTTTGGTTTCCCCAAAAAGCCTCATACCAATTGAGATATTGTTCCTTACTTGTAAACCCACGATCTTCCTCTTAATTAGCCAAGGTAGGACTACTTCTTTCAATAATTCTCAACTGCTCCTAATGTTCATAAATCATACCTATGAACATCATGAACGCACTCTTTCTGTTACAAGGTAATCATTCCTCCATGATGCAAAACAATGACGATGTCATTTGAATTAGAGTGATGGTTAAAGTGTCTGTTCTTAATCCGCTCGAATCATCTTATTATCATTATTATTAATTTTTTATGCATTAGGCCTTGAGTGACCTTCATCTGGAAATCCTGCTTTTTATCATTCTATTGATATTGTCTTTTGCATTTCGTTTCTGGAAAACAGGACCCAAGGAGTTCATTGCTCTGTTTTATGGTCTTCTAAGCTGGAGGGTAAGCTGAAGATTTAATATCATTTCAGCCAACATTGTCTAACTGACTATTTATTGGATCTTTTTCAGGGTATTTTTGAGAGATTTGATTCAGACCGAAGTGGAAAAATTGATTCCAGAGAGTTGAAAGAAGCACTTTTCAGTTTGGGCTTTTCTGTCTCACCAATGATCTTGGACTTGCTGGTGTCTAAGTTTGACAAAAGTGGTGGGAATAGCAAGGCCATTGAGTATGATAATTTTATAGAGTACGTTTGTCTTCCTTACGTTTTCTTTTTATAACTGGGAGTTGAAGCTTTGTTCCCATGTCCCGGGGGGCACGCACACCCGCCCCAAGGGCCGGACCCGGGAGACATCAAGGGTCTTTGGTATTAGTTTGTCTTCCTTACTCTTGATTTTACGTGGTACTCCTGATGAGAGTTTCTGAGTTACCCTTTAGTGTTGAGTTTTCACTTCACATTAATCACTGTTCTTATCAATTCTTTTTATTTTTGCAATTGCAGGTGTTGTCTTACAGTCAAGGTGAGCTTTTTTTATTTGCATGTCTTTTGTATACTACAGTCTATACTCTTTAGAGGTGATGACATGATCATTGAATCCGTGTATTTAGTCATGCATATTTGTTTAGTTGTCTTGATTTTGTTCCACTCATGTTGATGCCTATGAATACCTCGAAATACCAGTGTAACAATCCAAGCCCATCATTAGCAAATATTCTCTGTTTCGGCACGTTACGTATTGCCGTTAGCTTCATGATTTTAAAATGTGTCTGCTAGAGAGAGGTTTTCACACCCTTAGAAAGAATGCTTCGTTCCCCTCTCCAACCGATGTGAGATCTCACAATTCACTCCCTTTTGGGGCTAGCGTCCTCGTTGATACTCGTTCCCCTCTCCAATCAACGTGGAATCTCACAATCCAGTTCCCTTCGGGACTCAATGTCCTCGCTAGCACACTGCTCGATGCCTAGCTTTGATACCAGTGTCCTCGTTAGCACACCGCCCAAACCCACCACTAGCAGATATTGTCCGTTCAGCCCGTTACGTATCGCCATTAGCCTCACGGTTTTAAAACGTGTTTGCTAGGGAGAGGTTTCTACACCCTTATAAACAATGCCTCCTCCCCAACCGATGTGAGATCTCACAATCCACCTCCCTTCGGGGCCAGCATCCTCGCTGACACTCATTCCCCTCTCCAATCGATATAGGATCTCACAACCTGTTACACCATTTGAACACCCATGGCTATCAAGTAGTTACTTACCATCTAATTTCTCATTACTACTATTGTTTTTTGTTTTTCTCCTTTCATGTAACATGCAATGGTCTTTCACACACAGTTATTTTGAACTCCTTGAATGAACCGTCAACCCGATCCTTCCAAAACAAAGCCATTTCGATCTTTGTGACTAAAACTCGAAGTGACCGCGATGTAAAAATTTACAGGGGCTGACCGAAAAATTCAAGGAAAAGGACACCATGTACTCTGGTTCAGCAACCTTCAGTTACGAGGCTTTCATGTTGACAGTCCTACCATTTCTCATTGCCTGAGGAGTTCTTCAGCCTTCGTTCCATCAGGTACGTTCAATATAAAGCCTCGAGTGTTCTTTGGCTTTTCCTTCTGATTTCCATTGTGTTTGATAGAATATATTTATGCAGCCTTATCCTTATCCTATCCCAGATTTCCAATTTCTCATTTGACCCTAAAATTTAGGTCATTGTGTCATATATGATGTAATGTGCAACGATTCATTGGGCTAAGGGTATTATTGAATACTCTTGCCCTTACGGGTATAAATGATTCTTGGGAATACATTATATTCCAATTACTCTC

mRNA sequence

ATGTCAGGTTACCGAAATCAACCTTCCGGTTACGGTTATAGTTACGGCGGTGGTGGCGGTTACGGAGCTCCACCGCCGACGTCTCAGCCGTACAGTTCTCCACCCTACGGCTCGCAACATCCTCCGTCACAGTCTCAGCAGCCGTACGCTCCGGTGGCCGCGCAATATGGCGCACCGTCGGCACCGTATGGATCATCTCCGGGCGAAAAACCGCCCAAGGACAAGCCTAATCAGTCTTACGGCGGATCAGCCAGCGGTGGTCATGGTTATCCACCGCCTTCTGCGTATGGAAGCCCATTTGCCTCACTCATGCCCTCCGCCTTTCCGCCTGGGACTGATCCGAGCATCGTTGCGTGTTTTCAAGTAGCCGATCGGGATAGGAGTGGGTTTATTGATGATAAGGAGTTGCAAGGTGTTCTGTCATCGTATAATCAGAAGTTTAGCATGAGAACTGTTCACCTCCTGATGTTTCATTTCACCAACACAAGCACCAGGAAGATCGGACCCAAGGAGTTCATTGCTCTGTTTTATGGTCTTCTAAGCTGGAGGGGTATTTTTGAGAGATTTGATTCAGACCGAAGTGGAAAAATTGATTCCAGAGAGTTGAAAGAAGCACTTTTCAGTTTGGGCTTTTCTGTCTCACCAATGATCTTGGACTTGCTGGTGTCTAAGTTTGACAAAAGTGGTGGGAATAGCAAGGCCATTGAGTATGATAATTTTATAGAGTGTTGTCTTACAGTCAAGGGGCTGACCGAAAAATTCAAGGAAAAGGACACCATGTACTCTGGTTCAGCAACCTTCAGTTACGAGGCTTTCATGTTGACAGTCCTACCATTTCTCATTGCCTGAGGAGTTCTTCAGCCTTCGTTCCATCAGGTACGTTCAATATAAAGCCTCGAGTGTTCTTTGGCTTTTCCTTCTGATTTCCATTGTGTTTGATAGAATATATTTATGCAGCCTTATCCTTATCCTATCCCAGATTTCCAATTTCTCATTTGACCCTAAAATTTAGGTCATTGTGTCATATATGATGTAATGTGCAACGATTCATTGGGCTAAGGGTATTATTGAATACTCTTGCCCTTACGGGTATAAATGATTCTTGGGAATACATTATATTCCAATTACTCTC

Coding sequence (CDS)

ATGTCAGGTTACCGAAATCAACCTTCCGGTTACGGTTATAGTTACGGCGGTGGTGGCGGTTACGGAGCTCCACCGCCGACGTCTCAGCCGTACAGTTCTCCACCCTACGGCTCGCAACATCCTCCGTCACAGTCTCAGCAGCCGTACGCTCCGGTGGCCGCGCAATATGGCGCACCGTCGGCACCGTATGGATCATCTCCGGGCGAAAAACCGCCCAAGGACAAGCCTAATCAGTCTTACGGCGGATCAGCCAGCGGTGGTCATGGTTATCCACCGCCTTCTGCGTATGGAAGCCCATTTGCCTCACTCATGCCCTCCGCCTTTCCGCCTGGGACTGATCCGAGCATCGTTGCGTGTTTTCAAGTAGCCGATCGGGATAGGAGTGGGTTTATTGATGATAAGGAGTTGCAAGGTGTTCTGTCATCGTATAATCAGAAGTTTAGCATGAGAACTGTTCACCTCCTGATGTTTCATTTCACCAACACAAGCACCAGGAAGATCGGACCCAAGGAGTTCATTGCTCTGTTTTATGGTCTTCTAAGCTGGAGGGGTATTTTTGAGAGATTTGATTCAGACCGAAGTGGAAAAATTGATTCCAGAGAGTTGAAAGAAGCACTTTTCAGTTTGGGCTTTTCTGTCTCACCAATGATCTTGGACTTGCTGGTGTCTAAGTTTGACAAAAGTGGTGGGAATAGCAAGGCCATTGAGTATGATAATTTTATAGAGTGTTGTCTTACAGTCAAGGGGCTGACCGAAAAATTCAAGGAAAAGGACACCATGTACTCTGGTTCAGCAACCTTCAGTTACGAGGCTTTCATGTTGACAGTCCTACCATTTCTCATTGCCTGA

Protein sequence

MSGYRNQPSGYGYSYGGGGGYGAPPPTSQPYSSPPYGSQHPPSQSQQPYAPVAAQYGAPSAPYGSSPGEKPPKDKPNQSYGGSASGGHGYPPPSAYGSPFASLMPSAFPPGTDPSIVACFQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNTSTRKIGPKEFIALFYGLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNSKAIEYDNFIECCLTVKGLTEKFKEKDTMYSGSATFSYEAFMLTVLPFLIA
BLAST of CmaCh03G000450 vs. Swiss-Prot
Match: CML49_ARATH (Probable calcium-binding protein CML49 OS=Arabidopsis thaliana GN=CML49 PE=2 SV=1)

HSP 1 Score: 329.3 bits (843), Expect = 4.0e-89
Identity = 199/335 (59.40%), Postives = 225/335 (67.16%), Query Frame = 1

Query: 1   MSGYRNQPSGYGYS---------YG---------GGGGYGAPPPTSQPYSSP---PYGSQ 60
           MSGY     GYGY          YG         G  G   PPP     SSP   PYG+Q
Sbjct: 1   MSGYPPSSQGYGYGGNPPPPQPPYGSTGNNPPPYGSSGSNPPPPYGSSASSPYAVPYGAQ 60

Query: 61  HPP--SQSQQPYA-------------PVAAQYGAPS-APYGSSPGEKPPK-----DKPNQ 120
             P  +    PYA             P  A YG+PS   YG+ P   P         P Q
Sbjct: 61  PAPYGAPPSAPYASLPGDHNKPHKEKPHGASYGSPSPGGYGAHPSSGPSDYGGYGGAPQQ 120

Query: 121 S-----YGGSAS-----GGHGYPPP-SAYGSPFASLMPSAFPPGTDPSIVACFQVADRDR 180
           S     YGG+       GG+G PPP ++YGSPFASL+PSAFPPGTDP+IVACFQ ADRD 
Sbjct: 121 SGHGGGYGGAPQQSGHGGGYGAPPPQASYGSPFASLVPSAFPPGTDPNIVACFQAADRDN 180

Query: 181 SGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNTSTRKIGPKEFIALFYGLLSWRGIFE 240
           SGFIDDKELQG LSSYNQ FS+RTVHLLM+ FTN++ RKIGPKEF +LF+ L +WR IFE
Sbjct: 181 SGFIDDKELQGALSSYNQSFSIRTVHLLMYLFTNSNVRKIGPKEFTSLFFSLQNWRSIFE 240

Query: 241 RFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNSKAIEYDNFIECCLTV 283
           RFD DRSG+ID+ EL++AL SLGFSVSP+ILDLLVSKFDKSGG ++AIEYDNFIECCLTV
Sbjct: 241 RFDKDRSGRIDTNELRDALMSLGFSVSPVILDLLVSKFDKSGGRNRAIEYDNFIECCLTV 300

BLAST of CmaCh03G000450 vs. Swiss-Prot
Match: CML50_ARATH (Probable calcium-binding protein CML50 OS=Arabidopsis thaliana GN=CML50 PE=2 SV=1)

HSP 1 Score: 322.4 bits (825), Expect = 4.9e-87
Identity = 176/269 (65.43%), Postives = 200/269 (74.35%), Query Frame = 1

Query: 18  GGGYGAPPPTSQP----YSSPPYGSQHPPSQSQQPYAPVAAQYGAPSAPYGSSPGEKPPK 77
           GGGYGAPPP+       Y + P  SQ  PS     Y        +    YG +P      
Sbjct: 96  GGGYGAPPPSGSSDYGSYGAGPRPSQ--PSGHGGGYGATPPHGVSDYGSYGGAP------ 155

Query: 78  DKPNQSYGGSASGGHGYPPPSAYGSPFASLMPSAFPPGTDPSIVACFQVADRDRSGFIDD 137
             P  +  G   G  GYPP ++YGSPFASL+PS F PGTDP+IVACFQ AD+D SGFIDD
Sbjct: 156 --PRPASSGHGGGYGGYPPQASYGSPFASLIPSGFAPGTDPNIVACFQAADQDGSGFIDD 215

Query: 138 KELQGVLSSYNQKFSMRTVHLLMFHFTNTSTRKIGPKEFIALFYGLLSWRGIFERFDSDR 197
           KELQG LSSY Q+FSMRTVHLLM+ FTN++  KIGPKEF ALFY L +WR IFER D DR
Sbjct: 216 KELQGALSSYQQRFSMRTVHLLMYLFTNSNAMKIGPKEFTALFYSLQNWRSIFERSDKDR 275

Query: 198 SGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNSKAIEYDNFIECCLTVKGLTEK 257
           SG+ID  EL++AL SLGFSVSP++LDLLVSKFDKSGG ++AIEYDNFIECCLTVKGLTEK
Sbjct: 276 SGRIDVNELRDALLSLGFSVSPVVLDLLVSKFDKSGGKNRAIEYDNFIECCLTVKGLTEK 335

Query: 258 FKEKDTMYSGSATFSYEAFMLTVLPFLIA 283
           FKEKDT YSGSATF+YE+FMLTVLPFLIA
Sbjct: 336 FKEKDTAYSGSATFNYESFMLTVLPFLIA 354

BLAST of CmaCh03G000450 vs. Swiss-Prot
Match: CML48_ARATH (Probable calcium-binding protein CML48 OS=Arabidopsis thaliana GN=CML48 PE=2 SV=2)

HSP 1 Score: 152.1 bits (383), Expect = 8.8e-36
Identity = 92/228 (40.35%), Postives = 138/228 (60.53%), Query Frame = 1

Query: 58  APSAPYGSSPGEKPPKDKPNQSYGGSASGGHGYPP--PSAYGSPFASLMPSAFPPGTDPS 117
           APSAP       + P+    Q + G +   + YP   P+   S ++ +    F P T P 
Sbjct: 8   APSAP-------ELPESFVQQQHDGESRYTYAYPSYQPTQQFSSYSGM----FSPETHPE 67

Query: 118 IVACFQVADRDRSGFIDDKELQGVLS-SYNQKFSMRTVHLLMFHFTNT--STRKIGPKEF 177
           IV  F+ ADR+RSGF+++ EL+  LS S     S RT+ LL+F +     S  ++GPKE+
Sbjct: 68  IVRSFESADRNRSGFLEESELRQALSLSGYDGISNRTIRLLLFIYKIPVDSLLRLGPKEY 127

Query: 178 IALFYGLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNS 237
           + L+  L  WR IF R+D DRSGK++S +L++A ++LG  +   +  L+VS+FD   G +
Sbjct: 128 VELWNCLAQWRAIFNRYDRDRSGKMNSTQLRDAFYNLGCVLPTSVHQLIVSQFDDGTGKT 187

Query: 238 KAIEYDNFIECCLTVKGLTEKFKEKDTMYSGSATFSYEAFMLTVLPFL 281
             + +D+F+EC + VKGLTEKF+E D  Y+G AT SY+ FML V+PF+
Sbjct: 188 VDLCFDSFLECGMIVKGLTEKFRENDPGYTGYATLSYDVFMLMVIPFI 224

BLAST of CmaCh03G000450 vs. Swiss-Prot
Match: PEF1_HUMAN (Peflin OS=Homo sapiens GN=PEF1 PE=1 SV=1)

HSP 1 Score: 131.0 bits (328), Expect = 2.1e-29
Identity = 100/277 (36.10%), Postives = 140/277 (50.54%), Query Frame = 1

Query: 9   SGYGYSYGGGGGYGAPP--PTSQPYSSPP-----YGSQHPPSQSQQPYAPVAAQYGAPSA 68
           + Y Y  G  G  G  P  P    Y  PP     YGS  PP       AP    YG P+ 
Sbjct: 2   ASYPYRQGCPGAAGQAPGAPPGSYYPGPPNSGGQYGSGLPPGGGYGGPAP-GGPYGPPAG 61

Query: 69  --PYGSSPGEKPPKDKPNQSYGGSASGG-HGYPPPSAYGSPFASLM-PSAFPPGTDPSIV 128
             PYG       P   P   YGG+A GG +G PPPS+YG+    L      PP  DP   
Sbjct: 62  GGPYGHPNPGMFPSGTPGGPYGGAAPGGPYGQPPPSSYGAQQPGLYGQGGAPPNVDPEAY 121

Query: 129 ACFQVADRDRSGFIDDKELQGVLSSYN-QKFSMRTVHLLMFHFTNTSTRKIGPKEFIALF 188
           + FQ  D D SG+I  KEL+  L + N   F+  T  +++  F  T + +I    F AL+
Sbjct: 122 SWFQSVDSDHSGYISMKELKQALVNCNWSSFNDETCLMMINMFDKTKSGRIDVYGFSALW 181

Query: 189 YGLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNSKAIE 248
             +  W+ +F+++D DRSG I   EL++AL  +G+++SP    LLVS++     N  A++
Sbjct: 182 KFIQQWKNLFQQYDRDRSGSISYTELQQALSQMGYNLSPQFTQLLVSRYCPRSAN-PAMQ 241

Query: 249 YDNFIECCLTVKGLTEKFKEKDTMYSGSATFSYEAFM 274
            D FI+ C  ++ LTE F+EKDT   G+   S+E F+
Sbjct: 242 LDRFIQVCTQLQVLTEAFREKDTAVQGNIRLSFEDFV 276

BLAST of CmaCh03G000450 vs. Swiss-Prot
Match: PEF1_XENLA (Peflin OS=Xenopus laevis GN=pef1 PE=1 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 3.0e-28
Identity = 95/271 (35.06%), Postives = 137/271 (50.55%), Query Frame = 1

Query: 12  GYSYGGG----GGYGAPPPTSQPYSSPPYGSQ--HPPSQSQQPYAPVAAQYG--APSAPY 71
           G  YGGG      YG P P + PY SPP G    HP   S  P AP    YG  AP  PY
Sbjct: 28  GQQYGGGVQPAASYGRPAPGA-PYGSPPSGGVYGHPVPGSAAPGAP-GGPYGGQAPGGPY 87

Query: 72  GSSPGEKPPKDKPNQSYGGSASGGHGYPPPSAYGSPFASLMPSAFPPGTDPSIVACFQVA 131
            S PG  P   + + SYG  A  G+                    PPG DP   + FQ  
Sbjct: 88  -SVPGSTPYGSQQHGSYGQGAPAGN-------------------IPPGVDPEAFSWFQTV 147

Query: 132 DRDRSGFIDDKELQGVLSSYN-QKFSMRTVHLLMFHFTNTSTRKIGPKEFIALFYGLLSW 191
           D D SG+I  KEL+  L + N   F+  T  ++M  F  +++ +I    F AL+  +  W
Sbjct: 148 DTDHSGYISLKELKQALVNTNWSSFNDETCTMMMNMFDKSNSGRIDMFGFSALWRFIQQW 207

Query: 192 RGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNSKAIEYDNFIE 251
           R +F+++D DRSG I+  EL +AL  +G+ +SP  + +++S++ +       ++ D FI+
Sbjct: 208 RNLFQQYDRDRSGSINQGELHQALCQMGYQLSPQFVQIVMSRYAQRSA-QPGLQLDRFIQ 267

Query: 252 CCLTVKGLTEKFKEKDTMYSGSATFSYEAFM 274
            C  ++ +TE F+EKDT   G+A  SYE F+
Sbjct: 268 ICTQLQSMTEAFREKDTGQIGTAKLSYEDFI 275

BLAST of CmaCh03G000450 vs. TrEMBL
Match: A0A0A0L336_CUCSA (Ef-hand calcium binding protein OS=Cucumis sativus GN=Csa_4G341560 PE=4 SV=1)

HSP 1 Score: 486.5 bits (1251), Expect = 2.2e-134
Identity = 250/292 (85.62%), Postives = 264/292 (90.41%), Query Frame = 1

Query: 1   MSGYRNQPSGYGYSYGGGGGYGAPPPTSQPYSSPPYGSQHPPSQSQQPYAPVAAQYGAPS 60
           MSGY NQPSGYGY YG  GGYGAPPPT+QPY+S  YG  HP SQ+QQPYAPVAA YG PS
Sbjct: 1   MSGYPNQPSGYGYGYGSTGGYGAPPPTAQPYNSSAYG--HPQSQTQQPYAPVAAPYGVPS 60

Query: 61  APYGSSPGEKPPKDKPNQSYG----------GSASGGHGYPPPSAYGSPFASLMPSAFPP 120
           APYGS+ GEKPPKDKPNQSYG          G+ASGGHGYPPPSAYGSPFASL+PSAFPP
Sbjct: 61  APYGSAQGEKPPKDKPNQSYGGAGGGHQSYGGAASGGHGYPPPSAYGSPFASLLPSAFPP 120

Query: 121 GTDPSIVACFQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNTSTRKIGPK 180
           GTDPSIVACFQVAD+D SGFIDDKELQGVLSSYNQKFS+RTVHLLM+HFTNT+TRKIGPK
Sbjct: 121 GTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTRKIGPK 180

Query: 181 EFIALFYGLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGG 240
           EFI+LFYGL SWRGIFERFDSDRSGKIDS EL+EAL SLGF+VSPM+LDLLVSKFDKSGG
Sbjct: 181 EFISLFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPMVLDLLVSKFDKSGG 240

Query: 241 NSKAIEYDNFIECCLTVKGLTEKFKEKDTMYSGSATFSYEAFMLTVLPFLIA 283
            SKAIEYDNFIECCLTVKGLTEKFKEKDT YSGSATFSYEAFMLTVLPFLIA
Sbjct: 241 KSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 290

BLAST of CmaCh03G000450 vs. TrEMBL
Match: E2JF17_VITVI (EF-hand calcium-binding protein OS=Vitis vinifera GN=VIT_00s0252g00130 PE=2 SV=1)

HSP 1 Score: 377.1 bits (967), Expect = 1.9e-101
Identity = 203/288 (70.49%), Postives = 238/288 (82.64%), Query Frame = 1

Query: 1   MSGYRNQPSGYGYSYGGGGGYGAPPPTSQPYSSPPYGSQHPPSQSQQPYAPVAAQYGAPS 60
           MSGY + P G+GY      GYGAPPP +QPYS+ PYG+  P  Q  QPYAPVA  YGAPS
Sbjct: 1   MSGYPHAPPGHGY------GYGAPPP-AQPYSATPYGA--PAPQGYQPYAPVATPYGAPS 60

Query: 61  APYGSSPGEKPPKDKPNQSYGGSA-----SGGHGYPPPS-AYGSPFASLMPSAFPPGTDP 120
           APY ++PGEKPPK+  N+ +GGS+     S   GYPP +  +GSPFA L+PSAFPPGTDP
Sbjct: 61  APY-AAPGEKPPKE--NKPHGGSSHQPPPSSMGGYPPSAPGFGSPFALLVPSAFPPGTDP 120

Query: 121 SIVACFQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNTSTRKIGPKEFIA 180
           ++VACFQ+AD+D SGFIDDKELQG LSSYNQ+FS+RTVHLLM+ FTN++ RKIGPKEF A
Sbjct: 121 NVVACFQMADQDGSGFIDDKELQGALSSYNQRFSLRTVHLLMYLFTNSNARKIGPKEFTA 180

Query: 181 LFYGLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNSKA 240
           +FY L +WR IFE FD DRSGKI++ EL+EAL SLGF+VSP++LDLL+SKFDKSGG +KA
Sbjct: 181 VFYSLQNWRAIFENFDRDRSGKIEAGELREALMSLGFAVSPVVLDLLLSKFDKSGGRNKA 240

Query: 241 IEYDNFIECCLTVKGLTEKFKEKDTMYSGSATFSYEAFMLTVLPFLIA 283
           IEYDNFIECCLTVKGLTEKFKEKD+ +SGSATFSYE FMLTVLPFLIA
Sbjct: 241 IEYDNFIECCLTVKGLTEKFKEKDSSFSGSATFSYENFMLTVLPFLIA 276

BLAST of CmaCh03G000450 vs. TrEMBL
Match: V7BKJ4_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_007G250200g PE=4 SV=1)

HSP 1 Score: 374.0 bits (959), Expect = 1.6e-100
Identity = 200/287 (69.69%), Postives = 232/287 (80.84%), Query Frame = 1

Query: 1   MSGYRNQPSGYGYSYGG--GGGYGAPPPTSQPYSSPPYGSQH---PPSQSQQPYAPVAAQ 60
           MSGY NQP+GYGY YG      YGAPPP SQ Y +PP G  +   PP QS       A+ 
Sbjct: 1   MSGYPNQPTGYGYGYGAPPSSQYGAPPP-SQSYGAPPSGQSYGAPPPGQSYN-----ASA 60

Query: 61  YGAPSAPYGSSPGEKPPKDKPNQSYGGSASGGHGYPPPSAYGSPFASLMPSAFPPGTDPS 120
           YG PSAPY ++P  KP KD+ +   GG   GG GYPP ++YGSPFASL+PSAFPPGTDP+
Sbjct: 61  YGQPSAPY-AAPYSKPSKDESHSHGGG---GGGGYPPAASYGSPFASLVPSAFPPGTDPN 120

Query: 121 IVACFQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNTSTRKIGPKEFIAL 180
           +VACFQ+AD+D SGFIDDKELQ  LSSYNQ FS+RTVHLLM+HFTN++ +KIGPKEF +L
Sbjct: 121 VVACFQMADQDGSGFIDDKELQRALSSYNQSFSLRTVHLLMYHFTNSNVKKIGPKEFTSL 180

Query: 181 FYGLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNSKAI 240
           FY L +WR IFERFD DRSGKIDS EL++AL SLG++VSP++LDLLVSKFDK+GG SKAI
Sbjct: 181 FYSLQNWRSIFERFDKDRSGKIDSMELRDALLSLGYAVSPVVLDLLVSKFDKTGGKSKAI 240

Query: 241 EYDNFIECCLTVKGLTEKFKEKDTMYSGSATFSYEAFMLTVLPFLIA 283
           EYDNFIEC LTVKGLT+KFKEKDT Y+GSATFSYEAFMLTVLPFL+A
Sbjct: 241 EYDNFIECSLTVKGLTDKFKEKDTAYTGSATFSYEAFMLTVLPFLVA 277

BLAST of CmaCh03G000450 vs. TrEMBL
Match: A0A067JQC2_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_21125 PE=4 SV=1)

HSP 1 Score: 371.7 bits (953), Expect = 7.9e-100
Identity = 199/283 (70.32%), Postives = 226/283 (79.86%), Query Frame = 1

Query: 1   MSGYRNQPSGYGYSYGGGGGYGAPPPTSQPY-SSPPYGSQHPPSQSQQPYAPVAAQYGAP 60
           MSGY +QP  YGY       YGA P   QPY ++PPYG+Q P     QPY   +A YGAP
Sbjct: 1   MSGYPHQPPSYGYG-APQQPYGASPSPPQPYGAAPPYGAQPPT----QPYGGPSAPYGAP 60

Query: 61  SAPYGSSPGEKPPKDKPNQSYGGSASGGHGYPPPSAYGSPFASLMPSAFPPGTDPSIVAC 120
              Y   PG+KPPKDKP+ S  GS      YPP +AYGSPFASLMPSAFPPGTDP++VAC
Sbjct: 61  ---YAHPPGDKPPKDKPHSSAPGS------YPPSAAYGSPFASLMPSAFPPGTDPNVVAC 120

Query: 121 FQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNTSTRKIGPKEFIALFYGL 180
           FQ+AD+D SGFIDDKELQ  LS+YNQ FS+RTVHLLM+HFTNT+TRKIGPKEF  +FY L
Sbjct: 121 FQLADQDGSGFIDDKELQRALSTYNQSFSLRTVHLLMYHFTNTNTRKIGPKEFTQVFYSL 180

Query: 181 LSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNSKAIEYDN 240
            +WR IFERFD DRSG+ID  EL+EAL SLGF+VSP++LDLLVSKFDK+GG ++AIEYDN
Sbjct: 181 QNWRAIFERFDRDRSGRIDPNELREALLSLGFAVSPVVLDLLVSKFDKTGGKARAIEYDN 240

Query: 241 FIECCLTVKGLTEKFKEKDTMYSGSATFSYEAFMLTVLPFLIA 283
           FIECCLTVKGLTEKFKEKD  YSGSATF+YEAFMLTVLPFLIA
Sbjct: 241 FIECCLTVKGLTEKFKEKDATYSGSATFTYEAFMLTVLPFLIA 269

BLAST of CmaCh03G000450 vs. TrEMBL
Match: A0A059DI85_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_A02309 PE=4 SV=1)

HSP 1 Score: 370.5 bits (950), Expect = 1.8e-99
Identity = 201/287 (70.03%), Postives = 226/287 (78.75%), Query Frame = 1

Query: 1   MSGYRNQPSGYGYSYGGGGGYGAPPPTSQPYSSPPYGSQHPPSQSQQPYAPVAAQYGAPS 60
           M+GY N P  YGY        GAPPP        PYG+   P+  QQPY   AA YGAPS
Sbjct: 1   MAGYPNAPPNYGY--------GAPPP--------PYGAA--PAYGQQPYGAPAAPYGAPS 60

Query: 61  APYGSSP--GEKPPKDKPNQSYGGSASGGHGYPPP---SAYGSPFASLMPSAFPPGTDPS 120
           APYG+ P   +KP KDKP +   G+A+ G GYPPP   SAYG+PFA+LMPS FPPGTDPS
Sbjct: 61  APYGAPPPAADKPAKDKPPKDPYGAAAAG-GYPPPPGASAYGNPFAALMPSQFPPGTDPS 120

Query: 121 IVACFQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNTSTRKIGPKEFIAL 180
           +VA FQ+AD DRSGFIDDKELQ  LS+YNQ FS+RTVHLLM+ FTNT+ RKIGPKEF  +
Sbjct: 121 LVASFQMADLDRSGFIDDKELQRALSTYNQSFSLRTVHLLMYLFTNTNARKIGPKEFTQV 180

Query: 181 FYGLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNSKAI 240
           FY L +WRGIFERFD DRSGKID+ EL+EAL SLGFS+SP +LDLLVSKFDKSGG +KAI
Sbjct: 181 FYSLQNWRGIFERFDRDRSGKIDANELREALLSLGFSISPAVLDLLVSKFDKSGGKNKAI 240

Query: 241 EYDNFIECCLTVKGLTEKFKEKDTMYSGSATFSYEAFMLTVLPFLIA 283
           EYDNFIECCLTVKGLTEKFKE+DTMYSGSATF+YE FMLTVLPFLIA
Sbjct: 241 EYDNFIECCLTVKGLTEKFKERDTMYSGSATFNYEGFMLTVLPFLIA 268

BLAST of CmaCh03G000450 vs. TAIR10
Match: AT3G10300.3 (AT3G10300.3 Calcium-binding EF-hand family protein)

HSP 1 Score: 329.3 bits (843), Expect = 2.3e-90
Identity = 199/335 (59.40%), Postives = 225/335 (67.16%), Query Frame = 1

Query: 1   MSGYRNQPSGYGYS---------YG---------GGGGYGAPPPTSQPYSSP---PYGSQ 60
           MSGY     GYGY          YG         G  G   PPP     SSP   PYG+Q
Sbjct: 1   MSGYPPSSQGYGYGGNPPPPQPPYGSTGNNPPPYGSSGSNPPPPYGSSASSPYAVPYGAQ 60

Query: 61  HPP--SQSQQPYA-------------PVAAQYGAPS-APYGSSPGEKPPK-----DKPNQ 120
             P  +    PYA             P  A YG+PS   YG+ P   P         P Q
Sbjct: 61  PAPYGAPPSAPYASLPGDHNKPHKEKPHGASYGSPSPGGYGAHPSSGPSDYGGYGGAPQQ 120

Query: 121 S-----YGGSAS-----GGHGYPPP-SAYGSPFASLMPSAFPPGTDPSIVACFQVADRDR 180
           S     YGG+       GG+G PPP ++YGSPFASL+PSAFPPGTDP+IVACFQ ADRD 
Sbjct: 121 SGHGGGYGGAPQQSGHGGGYGAPPPQASYGSPFASLVPSAFPPGTDPNIVACFQAADRDN 180

Query: 181 SGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNTSTRKIGPKEFIALFYGLLSWRGIFE 240
           SGFIDDKELQG LSSYNQ FS+RTVHLLM+ FTN++ RKIGPKEF +LF+ L +WR IFE
Sbjct: 181 SGFIDDKELQGALSSYNQSFSIRTVHLLMYLFTNSNVRKIGPKEFTSLFFSLQNWRSIFE 240

Query: 241 RFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNSKAIEYDNFIECCLTV 283
           RFD DRSG+ID+ EL++AL SLGFSVSP+ILDLLVSKFDKSGG ++AIEYDNFIECCLTV
Sbjct: 241 RFDKDRSGRIDTNELRDALMSLGFSVSPVILDLLVSKFDKSGGRNRAIEYDNFIECCLTV 300

BLAST of CmaCh03G000450 vs. TAIR10
Match: AT5G04170.1 (AT5G04170.1 Calcium-binding EF-hand family protein)

HSP 1 Score: 322.4 bits (825), Expect = 2.8e-88
Identity = 176/269 (65.43%), Postives = 200/269 (74.35%), Query Frame = 1

Query: 18  GGGYGAPPPTSQP----YSSPPYGSQHPPSQSQQPYAPVAAQYGAPSAPYGSSPGEKPPK 77
           GGGYGAPPP+       Y + P  SQ  PS     Y        +    YG +P      
Sbjct: 96  GGGYGAPPPSGSSDYGSYGAGPRPSQ--PSGHGGGYGATPPHGVSDYGSYGGAP------ 155

Query: 78  DKPNQSYGGSASGGHGYPPPSAYGSPFASLMPSAFPPGTDPSIVACFQVADRDRSGFIDD 137
             P  +  G   G  GYPP ++YGSPFASL+PS F PGTDP+IVACFQ AD+D SGFIDD
Sbjct: 156 --PRPASSGHGGGYGGYPPQASYGSPFASLIPSGFAPGTDPNIVACFQAADQDGSGFIDD 215

Query: 138 KELQGVLSSYNQKFSMRTVHLLMFHFTNTSTRKIGPKEFIALFYGLLSWRGIFERFDSDR 197
           KELQG LSSY Q+FSMRTVHLLM+ FTN++  KIGPKEF ALFY L +WR IFER D DR
Sbjct: 216 KELQGALSSYQQRFSMRTVHLLMYLFTNSNAMKIGPKEFTALFYSLQNWRSIFERSDKDR 275

Query: 198 SGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNSKAIEYDNFIECCLTVKGLTEK 257
           SG+ID  EL++AL SLGFSVSP++LDLLVSKFDKSGG ++AIEYDNFIECCLTVKGLTEK
Sbjct: 276 SGRIDVNELRDALLSLGFSVSPVVLDLLVSKFDKSGGKNRAIEYDNFIECCLTVKGLTEK 335

Query: 258 FKEKDTMYSGSATFSYEAFMLTVLPFLIA 283
           FKEKDT YSGSATF+YE+FMLTVLPFLIA
Sbjct: 336 FKEKDTAYSGSATFNYESFMLTVLPFLIA 354

BLAST of CmaCh03G000450 vs. TAIR10
Match: AT2G27480.1 (AT2G27480.1 Calcium-binding EF-hand family protein)

HSP 1 Score: 152.1 bits (383), Expect = 5.0e-37
Identity = 92/228 (40.35%), Postives = 138/228 (60.53%), Query Frame = 1

Query: 58  APSAPYGSSPGEKPPKDKPNQSYGGSASGGHGYPP--PSAYGSPFASLMPSAFPPGTDPS 117
           APSAP       + P+    Q + G +   + YP   P+   S ++ +    F P T P 
Sbjct: 8   APSAP-------ELPESFVQQQHDGESRYTYAYPSYQPTQQFSSYSGM----FSPETHPE 67

Query: 118 IVACFQVADRDRSGFIDDKELQGVLS-SYNQKFSMRTVHLLMFHFTNT--STRKIGPKEF 177
           IV  F+ ADR+RSGF+++ EL+  LS S     S RT+ LL+F +     S  ++GPKE+
Sbjct: 68  IVRSFESADRNRSGFLEESELRQALSLSGYDGISNRTIRLLLFIYKIPVDSLLRLGPKEY 127

Query: 178 IALFYGLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNS 237
           + L+  L  WR IF R+D DRSGK++S +L++A ++LG  +   +  L+VS+FD   G +
Sbjct: 128 VELWNCLAQWRAIFNRYDRDRSGKMNSTQLRDAFYNLGCVLPTSVHQLIVSQFDDGTGKT 187

Query: 238 KAIEYDNFIECCLTVKGLTEKFKEKDTMYSGSATFSYEAFMLTVLPFL 281
             + +D+F+EC + VKGLTEKF+E D  Y+G AT SY+ FML V+PF+
Sbjct: 188 VDLCFDSFLECGMIVKGLTEKFRENDPGYTGYATLSYDVFMLMVIPFI 224

BLAST of CmaCh03G000450 vs. TAIR10
Match: AT4G37010.2 (AT4G37010.2 centrin 2)

HSP 1 Score: 52.8 bits (125), Expect = 4.1e-07
Identity = 32/85 (37.65%), Postives = 50/85 (58.82%), Query Frame = 1

Query: 183 RGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNSKAIEYDNFIE 242
           R IF+ FD D SG ID+ EL  A+ SLGF ++   ++ L+++ DK+   S AI++D F+ 
Sbjct: 33  REIFDLFDIDGSGSIDASELNVAMRSLGFEMNNQQINELMAEVDKN--QSGAIDFDEFVH 92

Query: 243 CCLTVKGLTEKFKEKDTMYSGSATF 268
                  +T KF E+D++   S  F
Sbjct: 93  M------MTTKFGERDSIDELSKAF 109

BLAST of CmaCh03G000450 vs. TAIR10
Match: AT3G50360.1 (AT3G50360.1 centrin2)

HSP 1 Score: 51.2 bits (121), Expect = 1.2e-06
Identity = 28/74 (37.84%), Postives = 45/74 (60.81%), Query Frame = 1

Query: 186 FERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNSKAIEYDNFIECCL 245
           FE FD+D SG ID++EL  A+ +LGF ++   ++ +++  DK G  S AI++D F+    
Sbjct: 32  FELFDTDGSGTIDAKELNVAMRALGFEMTEEQINKMIADVDKDG--SGAIDFDEFVHM-- 91

Query: 246 TVKGLTEKFKEKDT 260
               +T K  E+DT
Sbjct: 92  ----MTAKIGERDT 97

BLAST of CmaCh03G000450 vs. NCBI nr
Match: gi|449449801|ref|XP_004142653.1| (PREDICTED: probable calcium-binding protein CML49 [Cucumis sativus])

HSP 1 Score: 486.5 bits (1251), Expect = 3.2e-134
Identity = 250/292 (85.62%), Postives = 264/292 (90.41%), Query Frame = 1

Query: 1   MSGYRNQPSGYGYSYGGGGGYGAPPPTSQPYSSPPYGSQHPPSQSQQPYAPVAAQYGAPS 60
           MSGY NQPSGYGY YG  GGYGAPPPT+QPY+S  YG  HP SQ+QQPYAPVAA YG PS
Sbjct: 1   MSGYPNQPSGYGYGYGSTGGYGAPPPTAQPYNSSAYG--HPQSQTQQPYAPVAAPYGVPS 60

Query: 61  APYGSSPGEKPPKDKPNQSYG----------GSASGGHGYPPPSAYGSPFASLMPSAFPP 120
           APYGS+ GEKPPKDKPNQSYG          G+ASGGHGYPPPSAYGSPFASL+PSAFPP
Sbjct: 61  APYGSAQGEKPPKDKPNQSYGGAGGGHQSYGGAASGGHGYPPPSAYGSPFASLLPSAFPP 120

Query: 121 GTDPSIVACFQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNTSTRKIGPK 180
           GTDPSIVACFQVAD+D SGFIDDKELQGVLSSYNQKFS+RTVHLLM+HFTNT+TRKIGPK
Sbjct: 121 GTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTRKIGPK 180

Query: 181 EFIALFYGLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGG 240
           EFI+LFYGL SWRGIFERFDSDRSGKIDS EL+EAL SLGF+VSPM+LDLLVSKFDKSGG
Sbjct: 181 EFISLFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPMVLDLLVSKFDKSGG 240

Query: 241 NSKAIEYDNFIECCLTVKGLTEKFKEKDTMYSGSATFSYEAFMLTVLPFLIA 283
            SKAIEYDNFIECCLTVKGLTEKFKEKDT YSGSATFSYEAFMLTVLPFLIA
Sbjct: 241 KSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 290

BLAST of CmaCh03G000450 vs. NCBI nr
Match: gi|659129266|ref|XP_008464601.1| (PREDICTED: probable calcium-binding protein CML49 [Cucumis melo])

HSP 1 Score: 473.4 bits (1217), Expect = 2.8e-130
Identity = 247/294 (84.01%), Postives = 263/294 (89.46%), Query Frame = 1

Query: 1   MSGYRNQPSGYGYSYG--GGGGYGAPPPTSQPYSSPPYGSQHPPSQSQQPYAPVAAQYGA 60
           MSGY NQPSGYGY YG  G GGYGAPPPT+QPY+S  YG  HP SQ+QQPYAPVAA YG 
Sbjct: 1   MSGYPNQPSGYGYGYGYGGTGGYGAPPPTAQPYNSSAYG--HPQSQTQQPYAPVAAPYGG 60

Query: 61  PSAPYGSSPGEKPPKDKPNQSYGGS----------ASGGHGYPPPSAYGSPFASLMPSAF 120
           PSAPY S+ GEKPPKDKPNQSYGG+          A GGHGYPPPSAYG+PFASL+PSAF
Sbjct: 61  PSAPY-SAQGEKPPKDKPNQSYGGAGGGHQSYGGAAGGGHGYPPPSAYGNPFASLLPSAF 120

Query: 121 PPGTDPSIVACFQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNTSTRKIG 180
           PPGTDPSIVACFQVAD+D SGFIDDKELQGVLSSYNQKFS+RTVHLLM+HFTNT+TRKIG
Sbjct: 121 PPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTRKIG 180

Query: 181 PKEFIALFYGLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKS 240
           PKEFI+LFYGL SWRGIFERFDSDRSGKIDS EL++AL SLGF+VSPM+LDLLVSKFDKS
Sbjct: 181 PKEFISLFYGLQSWRGIFERFDSDRSGKIDSNELRDALLSLGFAVSPMVLDLLVSKFDKS 240

Query: 241 GGNSKAIEYDNFIECCLTVKGLTEKFKEKDTMYSGSATFSYEAFMLTVLPFLIA 283
           GG SKAIEYDNFIECCLTVKGLTEKFKEKDT YSGSATFSYEAFMLTVLPFLIA
Sbjct: 241 GGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 291

BLAST of CmaCh03G000450 vs. NCBI nr
Match: gi|526117928|ref|NP_001267893.1| (uncharacterized protein LOC100248601 [Vitis vinifera])

HSP 1 Score: 377.1 bits (967), Expect = 2.7e-101
Identity = 203/288 (70.49%), Postives = 238/288 (82.64%), Query Frame = 1

Query: 1   MSGYRNQPSGYGYSYGGGGGYGAPPPTSQPYSSPPYGSQHPPSQSQQPYAPVAAQYGAPS 60
           MSGY + P G+GY      GYGAPPP +QPYS+ PYG+  P  Q  QPYAPVA  YGAPS
Sbjct: 1   MSGYPHAPPGHGY------GYGAPPP-AQPYSATPYGA--PAPQGYQPYAPVATPYGAPS 60

Query: 61  APYGSSPGEKPPKDKPNQSYGGSA-----SGGHGYPPPS-AYGSPFASLMPSAFPPGTDP 120
           APY ++PGEKPPK+  N+ +GGS+     S   GYPP +  +GSPFA L+PSAFPPGTDP
Sbjct: 61  APY-AAPGEKPPKE--NKPHGGSSHQPPPSSMGGYPPSAPGFGSPFALLVPSAFPPGTDP 120

Query: 121 SIVACFQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNTSTRKIGPKEFIA 180
           ++VACFQ+AD+D SGFIDDKELQG LSSYNQ+FS+RTVHLLM+ FTN++ RKIGPKEF A
Sbjct: 121 NVVACFQMADQDGSGFIDDKELQGALSSYNQRFSLRTVHLLMYLFTNSNARKIGPKEFTA 180

Query: 181 LFYGLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNSKA 240
           +FY L +WR IFE FD DRSGKI++ EL+EAL SLGF+VSP++LDLL+SKFDKSGG +KA
Sbjct: 181 VFYSLQNWRAIFENFDRDRSGKIEAGELREALMSLGFAVSPVVLDLLLSKFDKSGGRNKA 240

Query: 241 IEYDNFIECCLTVKGLTEKFKEKDTMYSGSATFSYEAFMLTVLPFLIA 283
           IEYDNFIECCLTVKGLTEKFKEKD+ +SGSATFSYE FMLTVLPFLIA
Sbjct: 241 IEYDNFIECCLTVKGLTEKFKEKDSSFSGSATFSYENFMLTVLPFLIA 276

BLAST of CmaCh03G000450 vs. NCBI nr
Match: gi|593689950|ref|XP_007145576.1| (hypothetical protein PHAVU_007G250200g [Phaseolus vulgaris])

HSP 1 Score: 374.0 bits (959), Expect = 2.3e-100
Identity = 200/287 (69.69%), Postives = 232/287 (80.84%), Query Frame = 1

Query: 1   MSGYRNQPSGYGYSYGG--GGGYGAPPPTSQPYSSPPYGSQH---PPSQSQQPYAPVAAQ 60
           MSGY NQP+GYGY YG      YGAPPP SQ Y +PP G  +   PP QS       A+ 
Sbjct: 1   MSGYPNQPTGYGYGYGAPPSSQYGAPPP-SQSYGAPPSGQSYGAPPPGQSYN-----ASA 60

Query: 61  YGAPSAPYGSSPGEKPPKDKPNQSYGGSASGGHGYPPPSAYGSPFASLMPSAFPPGTDPS 120
           YG PSAPY ++P  KP KD+ +   GG   GG GYPP ++YGSPFASL+PSAFPPGTDP+
Sbjct: 61  YGQPSAPY-AAPYSKPSKDESHSHGGG---GGGGYPPAASYGSPFASLVPSAFPPGTDPN 120

Query: 121 IVACFQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNTSTRKIGPKEFIAL 180
           +VACFQ+AD+D SGFIDDKELQ  LSSYNQ FS+RTVHLLM+HFTN++ +KIGPKEF +L
Sbjct: 121 VVACFQMADQDGSGFIDDKELQRALSSYNQSFSLRTVHLLMYHFTNSNVKKIGPKEFTSL 180

Query: 181 FYGLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNSKAI 240
           FY L +WR IFERFD DRSGKIDS EL++AL SLG++VSP++LDLLVSKFDK+GG SKAI
Sbjct: 181 FYSLQNWRSIFERFDKDRSGKIDSMELRDALLSLGYAVSPVVLDLLVSKFDKTGGKSKAI 240

Query: 241 EYDNFIECCLTVKGLTEKFKEKDTMYSGSATFSYEAFMLTVLPFLIA 283
           EYDNFIEC LTVKGLT+KFKEKDT Y+GSATFSYEAFMLTVLPFL+A
Sbjct: 241 EYDNFIECSLTVKGLTDKFKEKDTAYTGSATFSYEAFMLTVLPFLVA 277

BLAST of CmaCh03G000450 vs. NCBI nr
Match: gi|802730522|ref|XP_012086224.1| (PREDICTED: probable calcium-binding protein CML49 [Jatropha curcas])

HSP 1 Score: 371.7 bits (953), Expect = 1.1e-99
Identity = 199/283 (70.32%), Postives = 226/283 (79.86%), Query Frame = 1

Query: 1   MSGYRNQPSGYGYSYGGGGGYGAPPPTSQPY-SSPPYGSQHPPSQSQQPYAPVAAQYGAP 60
           MSGY +QP  YGY       YGA P   QPY ++PPYG+Q P     QPY   +A YGAP
Sbjct: 1   MSGYPHQPPSYGYG-APQQPYGASPSPPQPYGAAPPYGAQPPT----QPYGGPSAPYGAP 60

Query: 61  SAPYGSSPGEKPPKDKPNQSYGGSASGGHGYPPPSAYGSPFASLMPSAFPPGTDPSIVAC 120
              Y   PG+KPPKDKP+ S  GS      YPP +AYGSPFASLMPSAFPPGTDP++VAC
Sbjct: 61  ---YAHPPGDKPPKDKPHSSAPGS------YPPSAAYGSPFASLMPSAFPPGTDPNVVAC 120

Query: 121 FQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNTSTRKIGPKEFIALFYGL 180
           FQ+AD+D SGFIDDKELQ  LS+YNQ FS+RTVHLLM+HFTNT+TRKIGPKEF  +FY L
Sbjct: 121 FQLADQDGSGFIDDKELQRALSTYNQSFSLRTVHLLMYHFTNTNTRKIGPKEFTQVFYSL 180

Query: 181 LSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNSKAIEYDN 240
            +WR IFERFD DRSG+ID  EL+EAL SLGF+VSP++LDLLVSKFDK+GG ++AIEYDN
Sbjct: 181 QNWRAIFERFDRDRSGRIDPNELREALLSLGFAVSPVVLDLLVSKFDKTGGKARAIEYDN 240

Query: 241 FIECCLTVKGLTEKFKEKDTMYSGSATFSYEAFMLTVLPFLIA 283
           FIECCLTVKGLTEKFKEKD  YSGSATF+YEAFMLTVLPFLIA
Sbjct: 241 FIECCLTVKGLTEKFKEKDATYSGSATFTYEAFMLTVLPFLIA 269

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
CML49_ARATH4.0e-8959.40Probable calcium-binding protein CML49 OS=Arabidopsis thaliana GN=CML49 PE=2 SV=... [more]
CML50_ARATH4.9e-8765.43Probable calcium-binding protein CML50 OS=Arabidopsis thaliana GN=CML50 PE=2 SV=... [more]
CML48_ARATH8.8e-3640.35Probable calcium-binding protein CML48 OS=Arabidopsis thaliana GN=CML48 PE=2 SV=... [more]
PEF1_HUMAN2.1e-2936.10Peflin OS=Homo sapiens GN=PEF1 PE=1 SV=1[more]
PEF1_XENLA3.0e-2835.06Peflin OS=Xenopus laevis GN=pef1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0L336_CUCSA2.2e-13485.62Ef-hand calcium binding protein OS=Cucumis sativus GN=Csa_4G341560 PE=4 SV=1[more]
E2JF17_VITVI1.9e-10170.49EF-hand calcium-binding protein OS=Vitis vinifera GN=VIT_00s0252g00130 PE=2 SV=1[more]
V7BKJ4_PHAVU1.6e-10069.69Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_007G250200g PE=4 SV=1[more]
A0A067JQC2_JATCU7.9e-10070.32Uncharacterized protein OS=Jatropha curcas GN=JCGZ_21125 PE=4 SV=1[more]
A0A059DI85_EUCGR1.8e-9970.03Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_A02309 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G10300.32.3e-9059.40 Calcium-binding EF-hand family protein[more]
AT5G04170.12.8e-8865.43 Calcium-binding EF-hand family protein[more]
AT2G27480.15.0e-3740.35 Calcium-binding EF-hand family protein[more]
AT4G37010.24.1e-0737.65 centrin 2[more]
AT3G50360.11.2e-0637.84 centrin2[more]
Match NameE-valueIdentityDescription
gi|449449801|ref|XP_004142653.1|3.2e-13485.62PREDICTED: probable calcium-binding protein CML49 [Cucumis sativus][more]
gi|659129266|ref|XP_008464601.1|2.8e-13084.01PREDICTED: probable calcium-binding protein CML49 [Cucumis melo][more]
gi|526117928|ref|NP_001267893.1|2.7e-10170.49uncharacterized protein LOC100248601 [Vitis vinifera][more]
gi|593689950|ref|XP_007145576.1|2.3e-10069.69hypothetical protein PHAVU_007G250200g [Phaseolus vulgaris][more]
gi|802730522|ref|XP_012086224.1|1.1e-9970.32PREDICTED: probable calcium-binding protein CML49 [Jatropha curcas][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR002048EF_hand_dom
IPR011992EF-hand-dom_pair
IPR018247EF_Hand_1_Ca_BS
Vocabulary: Molecular Function
TermDefinition
GO:0005509calcium ion binding
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0005509 calcium ion binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh03G000450.1CmaCh03G000450.1mRNA


Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainPFAMPF13202EF-hand_5coord: 116..139
score: 4.
IPR002048EF-hand domainPFAMPF13405EF-hand_6coord: 182..210
score: 1.
IPR002048EF-hand domainSMARTSM00054efh_1coord: 115..143
score: 0.19coord: 181..209
score: 0
IPR002048EF-hand domainPROFILEPS50222EF_HAND_2coord: 177..212
score: 11.752coord: 111..146
score: 10
IPR011992EF-hand domain pairGENE3DG3DSA:1.10.238.10coord: 119..277
score: 2.3
IPR011992EF-hand domain pairunknownSSF47473EF-handcoord: 109..277
score: 3.09
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 190..202
score: -coord: 124..136
scor
NoneNo IPR availablePANTHERPTHR10183CALPAINcoord: 89..282
score: 6.7