Cp4.1LG15g06250 (gene) Cucurbita pepo (Zucchini)

Overview
NameCp4.1LG15g06250
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
DescriptionPectinesterase
LocationCp4.1LG15 : 6985574 .. 6986913 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCTCCATCACACGGTGAAAAACACACCAATTTACTCACCTCTTCCTTGTTCTTTTCCGTTCACACAATTCTCGTTTGTCCTCTCGACTTGCTTGTTCTTGTTCTCTGCTTCTCCAACCAACTTTCAAGATGGCATCTTTCTCCTTTGTTGCATGTTTCTTTGTCTGCTTCATCAACTTTTTGGCATGTGTTGATTCCGTCCGTTTTAATGCAATTGTAGCATTAGACGGTTCGGGACACTACAAAAGAATTAGTGATGCAATAGCAGCTGCTCCAGACAACTCAAAAACGCGATTTTACATTCATGTAACTCCCGGAATTTACCGGGAACATATTCAAATTCCATCTACCAAAAAGTTTATTGCTTTGATCGGTGATAATGCATTCACAACCATTATCGTCGACAATAGAAGTAATGGCACTGGATTCTCGACCTCTGACTCTGCAACCCTTGGTAATTACTTTTATTCTATTTGAGAAAAATCAATTTTAGCTCTAAAATTTGGTTGAGTGTGTATTAAGTTGGAGCTAAAAAAAACTTAGAACACTATGTCCTAAGTCAAAATTAAGCCTCTTTTACAAAATGGGTTGATATATTATTTAAATCTTTACTGATTTCTTCACTTTTATTTGATACAGAGGTCAAAGGTAATAATTTTGTAGCCAAATATTTGAGCTTTGAGAATTCCGCAGGATCACAAAACCACCGAGCAGTGGCTGTCTTAGACCAAGCTGATTACACTGCTTATTACAAGTGTAGATTCTTGGGTTTTCAAGACACTCTATATTTAAAGGGACACAATAATTTTTTAAAAGAGTGCGATATCTATGGAACAGTTGACTTTATTTTTGGGAGTGGACTTGTTATGTTCCAAGATTGTAACATATACGCTCGATTTTCAAAAAATGAAATTGTAGTCACTGCCCAGTCCAAAGATCAACCAATGCGCAAAAGCGGCTTCTCCTTTCAAAACTGTATTGTCACAGTTTCGTCGGAGATTGGAGTCAATGAGGCCAAAATTAAAATCTATCTTGGTCGGCCATGGAGACAATACTCAAGTGTTGTGTTCATGGAATCGTTTTTAGATGACAATGTGCAACCAAAGGGATGGATGTTTTGGCAAGGAGTACCAGTAAATAAGCTCTTTTATGCTGAGTTCAACAATAGAGGTCCTGGTGCTAACACAAAACATCGAGTTCATTGGTCAGGTTTTCATGTAATTAGAAAAAAATTAGCAAAAAAATTTACGGTAGAAAAATTTATCAACGGGACTGATTGGTTGCCTGAAACTGGTGTACCTTTCAAACCTGGATTTTATTTTTAATGATATTCGACTCGTC

mRNA sequence

CCTCCATCACACGGTGAAAAACACACCAATTTACTCACCTCTTCCTTGTTCTTTTCCGTTCACACAATTCTCGTTTGTCCTCTCGACTTGCTTGTTCTTGTTCTCTGCTTCTCCAACCAACTTTCAAGATGGCATCTTTCTCCTTTGTTGCATGTTTCTTTGTCTGCTTCATCAACTTTTTGGCATGTGTTGATTCCGTCCGTTTTAATGCAATTGTAGCATTAGACGGTTCGGGACACTACAAAAGAATTAGTGATGCAATAGCAGCTGCTCCAGACAACTCAAAAACGCGATTTTACATTCATGTAACTCCCGGAATTTACCGGGAACATATTCAAATTCCATCTACCAAAAAGTTTATTGCTTTGATCGGTGATAATGCATTCACAACCATTATCGTCGACAATAGAAGTAATGGCACTGGATTCTCGACCTCTGACTCTGCAACCCTTGAGGTCAAAGGTAATAATTTTGTAGCCAAATATTTGAGCTTTGAGAATTCCGCAGGATCACAAAACCACCGAGCAGTGGCTGTCTTAGACCAAGCTGATTACACTGCTTATTACAAGTGTAGATTCTTGGGTTTTCAAGACACTCTATATTTAAAGGGACACAATAATTTTTTAAAAGAGTGCGATATCTATGGAACAGTTGACTTTATTTTTGGGAGTGGACTTGTTATGTTCCAAGATTGTAACATATACGCTCGATTTTCAAAAAATGAAATTGTAGTCACTGCCCAGTCCAAAGATCAACCAATGCGCAAAAGCGGCTTCTCCTTTCAAAACTGTATTGTCACAGTTTCGTCGGAGATTGGAGTCAATGAGGCCAAAATTAAAATCTATCTTGGTCGGCCATGGAGACAATACTCAAGTGTTGTGTTCATGGAATCGTTTTTAGATGACAATGTGCAACCAAAGGGATGGATGTTTTGGCAAGGAGTACCAGTAAATAAGCTCTTTTATGCTGAGTTCAACAATAGAGGTCCTGGTGCTAACACAAAACATCGAGTTCATTGGTCAGGTTTTCATGTAATTAGAAAAAAATTAGCAAAAAAATTTACGGTAGAAAAATTTATCAACGGGACTGATTGGTTGCCTGAAACTGGTGTACCTTTCAAACCTGGATTTTATTTTTAATGATATTCGACTCGTC

Coding sequence (CDS)

ATGGCATCTTTCTCCTTTGTTGCATGTTTCTTTGTCTGCTTCATCAACTTTTTGGCATGTGTTGATTCCGTCCGTTTTAATGCAATTGTAGCATTAGACGGTTCGGGACACTACAAAAGAATTAGTGATGCAATAGCAGCTGCTCCAGACAACTCAAAAACGCGATTTTACATTCATGTAACTCCCGGAATTTACCGGGAACATATTCAAATTCCATCTACCAAAAAGTTTATTGCTTTGATCGGTGATAATGCATTCACAACCATTATCGTCGACAATAGAAGTAATGGCACTGGATTCTCGACCTCTGACTCTGCAACCCTTGAGGTCAAAGGTAATAATTTTGTAGCCAAATATTTGAGCTTTGAGAATTCCGCAGGATCACAAAACCACCGAGCAGTGGCTGTCTTAGACCAAGCTGATTACACTGCTTATTACAAGTGTAGATTCTTGGGTTTTCAAGACACTCTATATTTAAAGGGACACAATAATTTTTTAAAAGAGTGCGATATCTATGGAACAGTTGACTTTATTTTTGGGAGTGGACTTGTTATGTTCCAAGATTGTAACATATACGCTCGATTTTCAAAAAATGAAATTGTAGTCACTGCCCAGTCCAAAGATCAACCAATGCGCAAAAGCGGCTTCTCCTTTCAAAACTGTATTGTCACAGTTTCGTCGGAGATTGGAGTCAATGAGGCCAAAATTAAAATCTATCTTGGTCGGCCATGGAGACAATACTCAAGTGTTGTGTTCATGGAATCGTTTTTAGATGACAATGTGCAACCAAAGGGATGGATGTTTTGGCAAGGAGTACCAGTAAATAAGCTCTTTTATGCTGAGTTCAACAATAGAGGTCCTGGTGCTAACACAAAACATCGAGTTCATTGGTCAGGTTTTCATGTAATTAGAAAAAAATTAGCAAAAAAATTTACGGTAGAAAAATTTATCAACGGGACTGATTGGTTGCCTGAAACTGGTGTACCTTTCAAACCTGGATTTTATTTTTAA

Protein sequence

MASFSFVACFFVCFINFLACVDSVRFNAIVALDGSGHYKRISDAIAAAPDNSKTRFYIHVTPGIYREHIQIPSTKKFIALIGDNAFTTIIVDNRSNGTGFSTSDSATLEVKGNNFVAKYLSFENSAGSQNHRAVAVLDQADYTAYYKCRFLGFQDTLYLKGHNNFLKECDIYGTVDFIFGSGLVMFQDCNIYARFSKNEIVVTAQSKDQPMRKSGFSFQNCIVTVSSEIGVNEAKIKIYLGRPWRQYSSVVFMESFLDDNVQPKGWMFWQGVPVNKLFYAEFNNRGPGANTKHRVHWSGFHVIRKKLAKKFTVEKFINGTDWLPETGVPFKPGFYF
Homology
[Close]
BLAST of Cp4.1LG15g06250 vs. Swiss-Prot
Match: PME2_CITSI (Pectinesterase 2 OS=Citrus sinensis GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 286.6 bits (732), Expect = 3.6e-76
Identity = 138/312 (44.23%), Postives = 199/312 (63.78%), Query Frame = 1 

Query: 25  RFNAIVALDGSGHYKRISDAIAAAPDNSKTRFYIHVTPGIYREHIQIPSTKKFIALIGDN 84
           R N +VA DGSG+ K I +A+AAA     +R+ I++  G Y E+I++    K I  +GD 
Sbjct: 200 RANIVVAQDGSGNVKTIQEAVAAASRAGGSRYVIYIKAGTYNENIEVKL--KNIMFVGDG 259

Query: 85  AFTTIIVDNRSNGTGFSTSDSATLEVKGNNFVAKYLSFENSAGSQNHRAVAVLDQADYTA 144
              TII  ++S G G +T  SAT+ V G+NF+A+ ++  N+AG  NH+AVA+   +D + 
Sbjct: 260 IGKTIITGSKSVGGGATTFKSATVAVVGDNFIARDITIRNTAGPNNHQAVALRSGSDLSV 319

Query: 145 YYKCRFLGFQDTLYLKGHNNFLKECDIYGTVDFIFGSGLVMFQDCNIYARFSKNEI-VVT 204
           +Y+C F G+QDTLY+     F +ECDIYGTVDFIFG+  V+ Q+CNI+AR   N    +T
Sbjct: 320 FYRCSFEGYQDTLYVHSQRQFYRECDIYGTVDFIFGNAAVVLQNCNIFARKPPNRTNTLT 379

Query: 205 AQSKDQPMRKSGFSFQNCIVTVSSEIGVNEAKIKIYLGRPWRQYSSVVFMESFLDDNVQP 264
           AQ +  P + +G    NC VT +S++   ++ +K +LGRPW+QYS  V++++FLD  + P
Sbjct: 380 AQGRTDPNQSTGIIIHNCRVTAASDLKPVQSSVKTFLGRPWKQYSRTVYIKTFLDSLINP 439

Query: 265 KGWMFWQG-VPVNKLFYAEFNNRGPGANTKHRVHWSGFHVIRK-KLAKKFTVEKFINGTD 324
            GWM W G   +N L+YAE+ N GPG++T +RV W G+HV+       +FTV  FI G  
Sbjct: 440 AGWMEWSGDFALNTLYYAEYMNTGPGSSTANRVKWRGYHVLTSPSQVSQFTVGNFIAGNS 499

Query: 325 WLPETGVPFKPG 334
           WLP T VPF  G
Sbjct: 500 WLPATNVPFTSG 509

[Close]
BLAST of Cp4.1LG15g06250 vs. Swiss-Prot
Match: PME7_ARATH (Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana GN=PME7 PE=2 SV=1)

HSP 1 Score: 283.9 bits (725), Expect = 2.3e-75
Identity = 135/310 (43.55%), Postives = 196/310 (63.23%), Query Frame = 1 

Query: 30  VALDGSGHYKRISDAIAAAPDN---SKTRFYIHVTPGIYREHIQIPSTKKFIALIGDNAF 89
           V+ DG+G++  I+ A+AAAP+N   S   F I+VT GIY E+I I   K+++ +IGD   
Sbjct: 267 VSQDGTGNFTNITAAVAAAPNNTDGSAGFFLIYVTAGIYEEYISIAKNKRYMMMIGDGIN 326

Query: 90  TTIIVDNRSNGTGFSTSDSATLEVKGNNFVAKYLSFENSAGSQNHRAVAVLDQADYTAYY 149
            T++  NRS   G++T +SAT  V   NFVA  ++F N+AG + H+AVA+   AD++ +Y
Sbjct: 327 QTVVTGNRSVVDGWTTFNSATFAVTAPNFVAVNITFRNTAGPEKHQAVALRSGADFSIFY 386

Query: 150 KCRFLGFQDTLYLKGHNNFLKECDIYGTVDFIFGSGLVMFQDCNIYAR--FSKNEIVVTA 209
            C F  +QDTLY      F +ECD+YGTVDFIFG+  V+FQ+CN+Y R         +TA
Sbjct: 387 SCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVFQNCNLYPRKPMPNQFNAITA 446

Query: 210 QSKDQPMRKSGFSFQNCIVTVSSEIGVNEAKIKIYLGRPWRQYSSVVFMESFLDDNVQPK 269
           Q +  P + +G S QNC +  + ++  +   +K YLGRPW++YS  V+M+S++D  V+P 
Sbjct: 447 QGRSDPNQNTGTSIQNCTIKPADDLVSSNYTVKTYLGRPWKEYSRTVYMQSYIDGFVEPV 506

Query: 270 GWMFWQG-VPVNKLFYAEFNNRGPGANTKHRVHWSGFHVIRKKLAKKFTVEKFINGTDWL 329
           GW  W G   ++ L+YAE+NN GPG+NT +RV W G+HVI    A  FTV       DW+
Sbjct: 507 GWREWNGDFALSTLYYAEYNNTGPGSNTTNRVTWPGYHVINSTDAANFTVTGLFIEADWI 566

Query: 330 PETGVPFKPG 334
            +TGVP+  G
Sbjct: 567 WKTGVPYTSG 576

[Close]
BLAST of Cp4.1LG15g06250 vs. Swiss-Prot
Match: PME40_ARATH (Probable pectinesterase/pectinesterase inhibitor 40 OS=Arabidopsis thaliana GN=PME40 PE=2 SV=1)

HSP 1 Score: 281.6 bits (719), Expect = 1.2e-74
Identity = 138/314 (43.95%), Postives = 199/314 (63.38%), Query Frame = 1 

Query: 21  VDSVRFNAIVALDGSGHYKRISDAIAAAPDNSKTRFYIHVTPGIYREHIQIPSTKKFIAL 80
           VD  + N +VA +G+G+Y  I +AI+AAP++S+TRF I++  G Y E+I+IP  K  I  
Sbjct: 201 VDETKVNLVVAQNGTGNYTTIGEAISAAPNSSETRFVIYIKCGEYFENIEIPREKTMIMF 260

Query: 81  IGDNAFTTIIVDNRSNGTGFSTSDSATLEVKGNNFVAKYLSFENSAGSQNHRAVAVLDQA 140
           IGD    T+I  NRS   G++   SAT+ V+G+ F+AK LSF N AG + H+AVA+   +
Sbjct: 261 IGDGIGRTVIKANRSYADGWTAFHSATVGVRGSGFIAKDLSFVNYAGPEKHQAVALRSSS 320

Query: 141 DYTAYYKCRFLGFQDTLYLKGHNNFLKECDIYGTVDFIFGSGLVMFQDCNIYAR--FSKN 200
           D +AYY+C F  +QDT+Y+  H  F +ECDIYGTVDFIFG   V+FQ+C++YAR      
Sbjct: 321 DLSAYYRCSFESYQDTIYVHSHKQFYRECDIYGTVDFIFGDASVVFQNCSLYARRPNPNQ 380

Query: 201 EIVVTAQSKDQPMRKSGFSFQNCIVTVSSEIGVNEAKIKIYLGRPWRQYSSVVFMESFLD 260
           +I+ TAQ ++     +G S  +  +  + ++   +A  K YLGRPW+ YS  V M+SF+D
Sbjct: 381 KIIYTAQGRENSREPTGISIISSRILAAPDLIPVQANFKAYLGRPWQLYSRTVIMKSFID 440

Query: 261 DNVQPKGWMFWQ-GVPVNKLFYAEFNNRGPGANTKHRVHWSGFHVIRK-KLAKKFTVEKF 320
           D V P GW+ W+    +  L+Y E+ N GPG+N  +RV W GF  I   + A +F+V  F
Sbjct: 441 DLVDPAGWLKWKDDFALETLYYGEYMNEGPGSNMTNRVQWPGFKRIETVEEASQFSVGPF 500

Query: 321 INGTDWLPETGVPF 331
           I+G  WL  T +PF
Sbjct: 501 IDGNKWLNSTRIPF 514

[Close]
BLAST of Cp4.1LG15g06250 vs. Swiss-Prot
Match: PME39_ARATH (Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana GN=PME39 PE=2 SV=1)

HSP 1 Score: 279.6 bits (714), Expect = 4.4e-74
Identity = 139/320 (43.44%), Postives = 202/320 (63.12%), Query Frame = 1 

Query: 19  ACVDSVRFNAIVALDGSGHYKRISDAIAAAPDNSKTRFYIHVTPGIYREHIQIPSTKKFI 78
           A V    +N  VA+DG+G++  I+DA+ AAP+ S+TRF I++  G Y E++++P  K  I
Sbjct: 213 APVQETNYNLSVAIDGTGNFTTINDAVFAAPNMSETRFIIYIKGGEYFENVELPKKKTMI 272

Query: 79  ALIGDNAFTTIIVDNRSNGTGFSTSDSATLEVKGNNFVAKYLSFENSAGSQNHRAVAVLD 138
             IGD    T+I  NRS   G+ST  + T+ VKG  ++AK +SF NSAG    +AVA   
Sbjct: 273 MFIGDGIGKTVIKANRSRIDGWSTFQTPTVGVKGKGYIAKDISFVNSAGPAKAQAVAFRS 332

Query: 139 QADYTAYYKCRFLGFQDTLYLKGHNNFLKECDIYGTVDFIFGSGLVMFQDCNIYARFSK- 198
            +D++A+Y+C F G+QDTLY+     F +ECDIYGT+DFIFG+  V+FQ+ ++YAR    
Sbjct: 333 GSDHSAFYRCEFDGYQDTLYVHSAKQFYRECDIYGTIDFIFGNAAVVFQNSSLYARKPNP 392

Query: 199 -NEIVVTAQSKDQPMRKSGFSFQNCIVTVSSEIGVNEAKIKIYLGRPWRQYSSVVFMESF 258
            ++I  TAQS++Q  + +G S  NC +  + ++   +   K YLGRPWR+YS  V ++SF
Sbjct: 393 GHKIAFTAQSRNQSDQPTGISILNCRILAAPDLIPVKENFKAYLGRPWRKYSRTVIIKSF 452

Query: 259 LDDNVQPKGWMFW-QGVPVNKLFYAEFNNRGPGANTKHRVHWSGFHVIRKKL-AKKFTVE 318
           +DD + P GW+   +   +  L+Y E+ N GPGAN   RV W GF  I  +  A +FTV 
Sbjct: 453 IDDLIHPAGWLEGKKDFALETLYYGEYMNEGPGANMAKRVTWPGFRRIENQTEATQFTVG 512

Query: 319 KFINGTDWLPETGVPFKPGF 335
            FI+G+ WL  TG+PF  GF
Sbjct: 513 PFIDGSTWLNSTGIPFSLGF 532

[Close]
BLAST of Cp4.1LG15g06250 vs. Swiss-Prot
Match: PME12_ARATH (Probable pectinesterase/pectinesterase inhibitor 12 OS=Arabidopsis thaliana GN=PME12 PE=2 SV=1)

HSP 1 Score: 279.3 bits (713), Expect = 5.7e-74
Identity = 135/308 (43.83%), Postives = 191/308 (62.01%), Query Frame = 1 

Query: 29  IVALDGSGHYKRISDAIAAAPDNSKTRFYIHVTPGIYREHIQIPSTKKFIALIGDNAFTT 88
           +VA DG+G++  I++AI+ AP+ S  R  I+V  G+Y E+I IP  K  I LIGD +  T
Sbjct: 239 VVAADGTGNFSTINEAISFAPNMSNDRVLIYVKEGVYDENIDIPIYKTNIVLIGDGSDVT 298

Query: 89  IIVDNRSNGTGFSTSDSATLEVKGNNFVAKYLSFENSAGSQNHRAVAVLDQADYTAYYKC 148
            I  NRS G G++T  SATL V G  F+A+ +   N+AG + H+AVA+   AD+ A Y+C
Sbjct: 299 FITGNRSVGDGWTTFRSATLAVSGEGFLARDIMITNTAGPEKHQAVALRVNADFVALYRC 358

Query: 149 RFLGFQDTLYLKGHNNFLKECDIYGTVDFIFGSGLVMFQDCNIYARFSK--NEIVVTAQS 208
              G+QDTLY      F +ECDIYGT+D+IFG+  V+FQ CNI ++        V+TAQS
Sbjct: 359 VIDGYQDTLYTHSFRQFYRECDIYGTIDYIFGNAAVVFQGCNIVSKLPMPGQFTVITAQS 418

Query: 209 KDQPMRKSGFSFQNCIVTVSSEIGVNEAKIKIYLGRPWRQYSSVVFMESFLDDNVQPKGW 268
           +D     +G S QNC +  S ++  +  K+K YLGRPWR++S  V MES++D+ +   GW
Sbjct: 419 RDTQDEDTGISMQNCSILASEDLFNSSNKVKSYLGRPWREFSRTVVMESYIDEFIDGSGW 478

Query: 269 MFWQ-GVPVNKLFYAEFNNRGPGANTKHRVHWSGFHVIRKKLAKKFTVEKFINGTDWLPE 328
             W  G  ++ L+Y E+NN GPG+ T  RV+W GFH++  + A  FT  +FI G  WL  
Sbjct: 479 SKWNGGEALDTLYYGEYNNNGPGSETVKRVNWPGFHIMGYEDAFNFTATEFITGDGWLGS 538

Query: 329 TGVPFKPG 334
           T  P+  G
Sbjct: 539 TSFPYDNG 546

[Close]
BLAST of Cp4.1LG15g06250 vs. TrEMBL
Match: A0A0A0LR14_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_1G049450 PE=4 SV=1)

HSP 1 Score: 448.0 bits (1151), Expect = 1.0e-122
Identity = 213/335 (63.58%), Postives = 260/335 (77.61%), Query Frame = 1 

Query: 1   MASFSFVACFFVCFINFLACVDSVRFNAIVALDGSGHYKRISDAIAAAPDNSKTRFYIHV 60
           M  F  V C   CF N +  + S+ FN  V+LDGSG++KRISDAIAAAP+NS TRFYIHV
Sbjct: 1   MLFFFGVPCSLFCFFNLMLLIHSLSFNLTVSLDGSGNFKRISDAIAAAPNNSNTRFYIHV 60

Query: 61  TPGIYREHIQIPSTKKFIALIGDNAFTTIIVDNRSNGTGFSTSDSATLEVKGNNFVAKYL 120
            PG Y E +QIP  KKFIAL+GD+ FTTII+DNRSNGTGF T++SATL V+G+NF+A+ L
Sbjct: 61  APGTYHETLQIPRLKKFIALVGDDPFTTIIIDNRSNGTGFKTNNSATLTVQGDNFMAESL 120

Query: 121 SFENSAGSQNHRAVAVLDQADYTAYYKCRFLGFQDTLYLKGHNNFLKECDIYGTVDFIFG 180
           +FENSAG QN++AVAV D+A++TAYYKCRFL FQDTLY+ G   F KE DIYG+VDFI G
Sbjct: 121 TFENSAGPQNNQAVAVFDKANHTAYYKCRFLSFQDTLYVNGKPQFFKESDIYGSVDFICG 180

Query: 181 SGLVMFQDCNIYARFSKNEIVVTAQSKDQPMRKSGFSFQNCIVTVSSEIGVNEAKIKIYL 240
            G VMFQDCNIYAR   N I VTAQSK      SGFSFQNC VTVS EI  N+  +K++L
Sbjct: 181 YGQVMFQDCNIYARMPINSITVTAQSKYILRSVSGFSFQNCTVTVSREISSNKQNVKVFL 240

Query: 241 GRPWRQYSSVVFMESFLDDNVQPKGWMFWQGVPVNKLFYAEFNNRGPGANTKHRVHWSGF 300
           GRPW+QYS VVFMESFLDD V  +GW+ W GVPVN LFY EFNN GPGA+   RV+W+ +
Sbjct: 241 GRPWKQYSKVVFMESFLDDVVASEGWVEWIGVPVNNLFYGEFNNCGPGADVSKRVNWTSY 300

Query: 301 HVIRKKLAKKFTVEKFINGTDWLPETGVPFKPGFY 336
           H++ K+ A +FTV+ F+NG++WLPETG+PF+ G +
Sbjct: 301 HLLDKESALRFTVDNFVNGSEWLPETGIPFRRGLH 335

[Close]
BLAST of Cp4.1LG15g06250 vs. TrEMBL
Match: A0A0A0L1M3_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_4G260430 PE=4 SV=1)

HSP 1 Score: 401.0 bits (1029), Expect = 1.4e-108
Identity = 192/314 (61.15%), Postives = 240/314 (76.43%), Query Frame = 1 

Query: 22  DSVRFNAIVALDGSGHYKRISDAIAAAPDNSKTRFYIHVTPGIYREHIQIPSTKKFIALI 81
           D   FN+ V+LDG+ ++ +I+DAIAAAP+ S TRFYIHV PG Y+E I++P  K  IALI
Sbjct: 17  DCFSFNSTVSLDGTENFIKINDAIAAAPNFSTTRFYIHVKPGTYQEIIEVPYEKTCIALI 76

Query: 82  GDNAFTTIIVDNRSNGTGFSTSDSATLEVKGNNFVAKYLSFENSAGSQNHRAVAVLDQAD 141
           GD++ TTIIV+NRSNGTG ST+ SATL V G+NF+A++L+F+N+AG    +A+A+LDQA 
Sbjct: 77  GDDSSTTIIVNNRSNGTGSSTASSATLTVNGSNFMAQFLTFQNTAGPNEGQAIALLDQAT 136

Query: 142 YTAYYKCRFLGFQDTLYLKGHNNFLKECDIYGTVDFIFGSGLVMFQDCNIYARFSKNEIV 201
           +TAYYKC FLG+QDTLY      F KECDIYG+VDFIFG+GLV+FQDCNIYAR    +I 
Sbjct: 137 HTAYYKCVFLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGLVVFQDCNIYARLFDVQIT 196

Query: 202 VTAQSKDQPMRKSGFSFQNCIVTVSSEIGVNEAKIKIYLGRPWRQYSSVVFMESFLDDNV 261
           VTAQSK      SGF FQNC VTVS EI  ++ K+ ++LGRPWR YS VVF++SFLD+ V
Sbjct: 197 VTAQSKPSLNSLSGFIFQNCKVTVSPEIASSKDKVTVFLGRPWRMYSMVVFIDSFLDNVV 256

Query: 262 QPKGWMFWQGVPVNKLFYAEFNNRGPGANTKHRVHWSGFHVIRK-KLAKKFTVEKFINGT 321
           QPKGW+ W GVP N L+YAE+NN G GANT  RV+W G+H +   K    FTVE FINGT
Sbjct: 257 QPKGWLEWPGVPENLLYYAEYNNSGGGANTSQRVNWPGYHALNNAKEVATFTVEIFINGT 316

Query: 322 DWLPETGVPFKPGF 335
            WLP+TG+PF+ GF
Sbjct: 317 QWLPQTGIPFRAGF 330

[Close]
BLAST of Cp4.1LG15g06250 vs. TrEMBL
Match: C0PT44_PICSI (Pectinesterase OS=Picea sitchensis PE=2 SV=1)

HSP 1 Score: 318.5 bits (815), Expect = 9.5e-84
Identity = 148/317 (46.69%), Postives = 209/317 (65.93%), Query Frame = 1 

Query: 22  DSVRFNAIVALDGSGHYKRISDAIAAAPDNSKTRFYIHVTPGIYREHIQIPSTKKFIALI 81
           D++  NA+VA DGSGH+  I DA+ AAP  S+TR+ IH+  GIY E++++   K  +  I
Sbjct: 284 DNIMPNAVVAKDGSGHHTSIGDAVNAAPQKSRTRYVIHIKAGIYWENVEVNKKKTHLMFI 343

Query: 82  GDNAFTTIIVDNRSNGTGFSTSDSATLEVKGNNFVAKYLSFENSAGSQNHRAVAVLDQAD 141
           GD    T++  NR+   G++T  SAT+ V GN F+A+ ++FEN+AG+  H+AVA+   +D
Sbjct: 344 GDGIGATVVAGNRNVKDGYTTYRSATVAVNGNGFIARDITFENTAGAAKHQAVALRVGSD 403

Query: 142 YTAYYKCRFLGFQDTLYLKGHNNFLKECDIYGTVDFIFGSGLVMFQDCNIYAR--FSKNE 201
           ++A+Y+C F G+QDTLY+     F +EC++YGTVDFIFG+  V+ Q+CN++AR   +  +
Sbjct: 404 FSAFYRCSFQGYQDTLYVHSLRQFYRECNVYGTVDFIFGNAAVVLQNCNLFARKPLANQQ 463

Query: 202 IVVTAQSKDQPMRKSGFSFQNCIVTVSSEIGVNEAKIKIYLGRPWRQYSSVVFMESFLDD 261
           IV TAQ +  P   +G S QNC V  +S++   +     YLGRPWRQYS  VFM+S+L D
Sbjct: 464 IVYTAQGRQDPNENTGISIQNCQVIAASDLIPVKRSFPAYLGRPWRQYSRTVFMQSYLGD 523

Query: 262 NVQPKGWMFWQG-VPVNKLFYAEFNNRGPGANTKHRVHWSGFHVIR-KKLAKKFTVEKFI 321
            +QP GW+ W G   +N L+Y EF NRGPGA   +RV W G+  IR    AK+FTV +FI
Sbjct: 524 LIQPAGWLEWNGNFALNTLYYGEFMNRGPGAGVANRVRWPGYRAIRSSNEAKQFTVSQFI 583

Query: 322 NGTDWLPETGVPFKPGF 335
            G  WLP TGV +  GF
Sbjct: 584 KGDSWLPSTGVKYVSGF 600

[Close]
BLAST of Cp4.1LG15g06250 vs. TrEMBL
Match: B0L3S3_TAICR (Pectinesterase OS=Taiwania cryptomerioides PE=2 SV=1)

HSP 1 Score: 312.4 bits (799), Expect = 6.8e-82
Identity = 145/318 (45.60%), Postives = 210/318 (66.04%), Query Frame = 1 

Query: 21  VDSVRFNAIVALDGSGHYKRISDAIAAAPDNSKTRFYIHVTPGIYREHIQIPSTKKFIAL 80
           V ++  NA+VA DGSGHY  IS A+ AAP+ S TRF I+V  G+Y+E+++I   K F+  
Sbjct: 276 VTNITANAVVAKDGSGHYSTISAAVDAAPEKSTTRFIIYVKKGVYQENVEIHKKKHFLMF 335

Query: 81  IGDNAFTTIIVDNRS-NGTGFSTSDSATLEVKGNNFVAKYLSFENSAGSQNHRAVAVLDQ 140
           IGD    T++  +RS  G+  +T  SAT+ V G  F+A+ ++FEN+AG  NH+AVA+   
Sbjct: 336 IGDGEGVTVVTASRSVRGSNHTTFHSATVAVTGKGFIARDMTFENTAGPSNHQAVALRVG 395

Query: 141 ADYTAYYKCRFLGFQDTLYLKGHNNFLKECDIYGTVDFIFGSGLVMFQDCNIYAR--FSK 200
           +D++ +Y+C F G+QDTLY+     F ++CDIYGTVDFIFG+  V+FQ+CN+YAR     
Sbjct: 396 SDFSVFYRCSFKGYQDTLYVHSLRQFFRDCDIYGTVDFIFGNAAVVFQNCNLYARKPLEN 455

Query: 201 NEIVVTAQSKDQPMRKSGFSFQNCIVTVSSEIGVNEAKIKIYLGRPWRQYSSVVFMESFL 260
            +I+ TAQ +  P + +G S  NC VT  S++   ++  K YLGRPW++YS  VF++S+L
Sbjct: 456 QQIMYTAQGRQDPNQNTGISIHNCRVTADSDMAAVKSSFKTYLGRPWKEYSRTVFLQSYL 515

Query: 261 DDNVQPKGWMFW-QGVPVNKLFYAEFNNRGPGANTKHRVHWSGFHVIRKKL-AKKFTVEK 320
           DD + P GW+ W +   ++ L+Y E+ N GPGA T +RV+W G+ VI     A +FTV +
Sbjct: 516 DDLIHPAGWLEWNETFALSTLYYGEYMNTGPGAGTANRVNWPGYRVITSATEASQFTVNQ 575

Query: 321 FINGTDWLPETGVPFKPG 334
           FI G  WLP TGV +  G
Sbjct: 576 FIEGDTWLPSTGVEYSSG 593

[Close]
BLAST of Cp4.1LG15g06250 vs. TrEMBL
Match: V7BGJ7_PHAVU (Pectinesterase OS=Phaseolus vulgaris GN=PHAVU_007G198200g PE=4 SV=1)

HSP 1 Score: 311.6 bits (797), Expect = 1.2e-81
Identity = 155/319 (48.59%), Postives = 207/319 (64.89%), Query Frame = 1 

Query: 24  VRFNAIVALDGSGHYKRISDAIAAAPDNSKTR---FYIHVTPGIYREHIQIPSTKKFIAL 83
           VR   +V  DGS  +  I+DA+AAAP+NS T      I+V  GIY E++ IP  K+ + L
Sbjct: 208 VRQKVVVNPDGSADFTTINDAVAAAPENSGTNNGYHVIYVVAGIYNEYVSIPKNKQNLML 267

Query: 84  IGDNAFTTIIVDNRSNGTGFSTSDSATLEVKGNNFVAKYLSFENSAGSQNHRAVAVLDQA 143
           IGD    T++  +RS   G++T  SAT  V G  FVA  ++F N+AGS  H+AVAV + A
Sbjct: 268 IGDGINRTVLTGDRSVVDGWTTFQSATFAVVGKGFVAVNITFRNTAGSSKHQAVAVRNGA 327

Query: 144 DYTAYYKCRFLGFQDTLYLKGHNNFLKECDIYGTVDFIFGSGLVMFQDCNIYARF-SKNE 203
           D + +Y C F G+QDTLY+     F K CDIYGTVDFIFG+   +FQDCNIY R   +N+
Sbjct: 328 DMSTFYNCSFEGYQDTLYVHSQRQFYKSCDIYGTVDFIFGNAATVFQDCNIYPRLPMQNQ 387

Query: 204 I-VVTAQSKDQPMRKSGFSFQNCIVTVSSEIG---VNEAKIKIYLGRPWRQYSSVVFMES 263
              +TAQ +  P + +G S QNC +T +SE+G    N   I+ YLGRPW++YS  V+++S
Sbjct: 388 FNAITAQGRTDPNQNTGISIQNCCITAASELGDATSNYNGIQTYLGRPWKEYSRTVYVQS 447

Query: 264 FLDDNVQPKGWMFWQG-VPVNKLFYAEFNNRGPGANTKHRVHWSGFHVIRKKLAKKFTVE 323
           F+D  + PKGW  W G   ++ L+YAEF N GPG+NT +RV W G+H+I +K A  FTV+
Sbjct: 448 FMDGLIDPKGWNEWSGDFALSTLYYAEFANWGPGSNTNNRVTWEGYHLIDEKDALDFTVK 507

Query: 324 KFINGTDWLPETGVPFKPG 334
           KFI G  WLP TGVPFK G
Sbjct: 508 KFIQGDKWLPTTGVPFKAG 526

[Close]
BLAST of Cp4.1LG15g06250 vs. TAIR10
Match: AT1G02810.1 (AT1G02810.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 283.9 bits (725), Expect = 1.3e-76
Identity = 135/310 (43.55%), Postives = 196/310 (63.23%), Query Frame = 1 

Query: 30  VALDGSGHYKRISDAIAAAPDN---SKTRFYIHVTPGIYREHIQIPSTKKFIALIGDNAF 89
           V+ DG+G++  I+ A+AAAP+N   S   F I+VT GIY E+I I   K+++ +IGD   
Sbjct: 267 VSQDGTGNFTNITAAVAAAPNNTDGSAGFFLIYVTAGIYEEYISIAKNKRYMMMIGDGIN 326

Query: 90  TTIIVDNRSNGTGFSTSDSATLEVKGNNFVAKYLSFENSAGSQNHRAVAVLDQADYTAYY 149
            T++  NRS   G++T +SAT  V   NFVA  ++F N+AG + H+AVA+   AD++ +Y
Sbjct: 327 QTVVTGNRSVVDGWTTFNSATFAVTAPNFVAVNITFRNTAGPEKHQAVALRSGADFSIFY 386

Query: 150 KCRFLGFQDTLYLKGHNNFLKECDIYGTVDFIFGSGLVMFQDCNIYAR--FSKNEIVVTA 209
            C F  +QDTLY      F +ECD+YGTVDFIFG+  V+FQ+CN+Y R         +TA
Sbjct: 387 SCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVFQNCNLYPRKPMPNQFNAITA 446

Query: 210 QSKDQPMRKSGFSFQNCIVTVSSEIGVNEAKIKIYLGRPWRQYSSVVFMESFLDDNVQPK 269
           Q +  P + +G S QNC +  + ++  +   +K YLGRPW++YS  V+M+S++D  V+P 
Sbjct: 447 QGRSDPNQNTGTSIQNCTIKPADDLVSSNYTVKTYLGRPWKEYSRTVYMQSYIDGFVEPV 506

Query: 270 GWMFWQG-VPVNKLFYAEFNNRGPGANTKHRVHWSGFHVIRKKLAKKFTVEKFINGTDWL 329
           GW  W G   ++ L+YAE+NN GPG+NT +RV W G+HVI    A  FTV       DW+
Sbjct: 507 GWREWNGDFALSTLYYAEYNNTGPGSNTTNRVTWPGYHVINSTDAANFTVTGLFIEADWI 566

Query: 330 PETGVPFKPG 334
            +TGVP+  G
Sbjct: 567 WKTGVPYTSG 576

[Close]
BLAST of Cp4.1LG15g06250 vs. TAIR10
Match: AT4G02320.1 (AT4G02320.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 281.6 bits (719), Expect = 6.5e-76
Identity = 138/314 (43.95%), Postives = 199/314 (63.38%), Query Frame = 1 

Query: 21  VDSVRFNAIVALDGSGHYKRISDAIAAAPDNSKTRFYIHVTPGIYREHIQIPSTKKFIAL 80
           VD  + N +VA +G+G+Y  I +AI+AAP++S+TRF I++  G Y E+I+IP  K  I  
Sbjct: 201 VDETKVNLVVAQNGTGNYTTIGEAISAAPNSSETRFVIYIKCGEYFENIEIPREKTMIMF 260

Query: 81  IGDNAFTTIIVDNRSNGTGFSTSDSATLEVKGNNFVAKYLSFENSAGSQNHRAVAVLDQA 140
           IGD    T+I  NRS   G++   SAT+ V+G+ F+AK LSF N AG + H+AVA+   +
Sbjct: 261 IGDGIGRTVIKANRSYADGWTAFHSATVGVRGSGFIAKDLSFVNYAGPEKHQAVALRSSS 320

Query: 141 DYTAYYKCRFLGFQDTLYLKGHNNFLKECDIYGTVDFIFGSGLVMFQDCNIYAR--FSKN 200
           D +AYY+C F  +QDT+Y+  H  F +ECDIYGTVDFIFG   V+FQ+C++YAR      
Sbjct: 321 DLSAYYRCSFESYQDTIYVHSHKQFYRECDIYGTVDFIFGDASVVFQNCSLYARRPNPNQ 380

Query: 201 EIVVTAQSKDQPMRKSGFSFQNCIVTVSSEIGVNEAKIKIYLGRPWRQYSSVVFMESFLD 260
           +I+ TAQ ++     +G S  +  +  + ++   +A  K YLGRPW+ YS  V M+SF+D
Sbjct: 381 KIIYTAQGRENSREPTGISIISSRILAAPDLIPVQANFKAYLGRPWQLYSRTVIMKSFID 440

Query: 261 DNVQPKGWMFWQ-GVPVNKLFYAEFNNRGPGANTKHRVHWSGFHVIRK-KLAKKFTVEKF 320
           D V P GW+ W+    +  L+Y E+ N GPG+N  +RV W GF  I   + A +F+V  F
Sbjct: 441 DLVDPAGWLKWKDDFALETLYYGEYMNEGPGSNMTNRVQWPGFKRIETVEEASQFSVGPF 500

Query: 321 INGTDWLPETGVPF 331
           I+G  WL  T +PF
Sbjct: 501 IDGNKWLNSTRIPF 514

[Close]
BLAST of Cp4.1LG15g06250 vs. TAIR10
Match: AT4G02300.1 (AT4G02300.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 279.6 bits (714), Expect = 2.5e-75
Identity = 139/320 (43.44%), Postives = 202/320 (63.12%), Query Frame = 1 

Query: 19  ACVDSVRFNAIVALDGSGHYKRISDAIAAAPDNSKTRFYIHVTPGIYREHIQIPSTKKFI 78
           A V    +N  VA+DG+G++  I+DA+ AAP+ S+TRF I++  G Y E++++P  K  I
Sbjct: 213 APVQETNYNLSVAIDGTGNFTTINDAVFAAPNMSETRFIIYIKGGEYFENVELPKKKTMI 272

Query: 79  ALIGDNAFTTIIVDNRSNGTGFSTSDSATLEVKGNNFVAKYLSFENSAGSQNHRAVAVLD 138
             IGD    T+I  NRS   G+ST  + T+ VKG  ++AK +SF NSAG    +AVA   
Sbjct: 273 MFIGDGIGKTVIKANRSRIDGWSTFQTPTVGVKGKGYIAKDISFVNSAGPAKAQAVAFRS 332

Query: 139 QADYTAYYKCRFLGFQDTLYLKGHNNFLKECDIYGTVDFIFGSGLVMFQDCNIYARFSK- 198
            +D++A+Y+C F G+QDTLY+     F +ECDIYGT+DFIFG+  V+FQ+ ++YAR    
Sbjct: 333 GSDHSAFYRCEFDGYQDTLYVHSAKQFYRECDIYGTIDFIFGNAAVVFQNSSLYARKPNP 392

Query: 199 -NEIVVTAQSKDQPMRKSGFSFQNCIVTVSSEIGVNEAKIKIYLGRPWRQYSSVVFMESF 258
            ++I  TAQS++Q  + +G S  NC +  + ++   +   K YLGRPWR+YS  V ++SF
Sbjct: 393 GHKIAFTAQSRNQSDQPTGISILNCRILAAPDLIPVKENFKAYLGRPWRKYSRTVIIKSF 452

Query: 259 LDDNVQPKGWMFW-QGVPVNKLFYAEFNNRGPGANTKHRVHWSGFHVIRKKL-AKKFTVE 318
           +DD + P GW+   +   +  L+Y E+ N GPGAN   RV W GF  I  +  A +FTV 
Sbjct: 453 IDDLIHPAGWLEGKKDFALETLYYGEYMNEGPGANMAKRVTWPGFRRIENQTEATQFTVG 512

Query: 319 KFINGTDWLPETGVPFKPGF 335
            FI+G+ WL  TG+PF  GF
Sbjct: 513 PFIDGSTWLNSTGIPFSLGF 532

[Close]
BLAST of Cp4.1LG15g06250 vs. TAIR10
Match: AT2G26440.1 (AT2G26440.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 279.3 bits (713), Expect = 3.2e-75
Identity = 135/308 (43.83%), Postives = 191/308 (62.01%), Query Frame = 1 

Query: 29  IVALDGSGHYKRISDAIAAAPDNSKTRFYIHVTPGIYREHIQIPSTKKFIALIGDNAFTT 88
           +VA DG+G++  I++AI+ AP+ S  R  I+V  G+Y E+I IP  K  I LIGD +  T
Sbjct: 239 VVAADGTGNFSTINEAISFAPNMSNDRVLIYVKEGVYDENIDIPIYKTNIVLIGDGSDVT 298

Query: 89  IIVDNRSNGTGFSTSDSATLEVKGNNFVAKYLSFENSAGSQNHRAVAVLDQADYTAYYKC 148
            I  NRS G G++T  SATL V G  F+A+ +   N+AG + H+AVA+   AD+ A Y+C
Sbjct: 299 FITGNRSVGDGWTTFRSATLAVSGEGFLARDIMITNTAGPEKHQAVALRVNADFVALYRC 358

Query: 149 RFLGFQDTLYLKGHNNFLKECDIYGTVDFIFGSGLVMFQDCNIYARFSK--NEIVVTAQS 208
              G+QDTLY      F +ECDIYGT+D+IFG+  V+FQ CNI ++        V+TAQS
Sbjct: 359 VIDGYQDTLYTHSFRQFYRECDIYGTIDYIFGNAAVVFQGCNIVSKLPMPGQFTVITAQS 418

Query: 209 KDQPMRKSGFSFQNCIVTVSSEIGVNEAKIKIYLGRPWRQYSSVVFMESFLDDNVQPKGW 268
           +D     +G S QNC +  S ++  +  K+K YLGRPWR++S  V MES++D+ +   GW
Sbjct: 419 RDTQDEDTGISMQNCSILASEDLFNSSNKVKSYLGRPWREFSRTVVMESYIDEFIDGSGW 478

Query: 269 MFWQ-GVPVNKLFYAEFNNRGPGANTKHRVHWSGFHVIRKKLAKKFTVEKFINGTDWLPE 328
             W  G  ++ L+Y E+NN GPG+ T  RV+W GFH++  + A  FT  +FI G  WL  
Sbjct: 479 SKWNGGEALDTLYYGEYNNNGPGSETVKRVNWPGFHIMGYEDAFNFTATEFITGDGWLGS 538

Query: 329 TGVPFKPG 334
           T  P+  G
Sbjct: 539 TSFPYDNG 546

[Close]
BLAST of Cp4.1LG15g06250 vs. TAIR10
Match: AT4G02330.1 (AT4G02330.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 277.3 bits (708), Expect = 1.2e-74
Identity = 132/321 (41.12%), Postives = 201/321 (62.62%), Query Frame = 1 

Query: 21  VDSVRFNAIVALD--GSGHYKRISDAIAAAP---DNSKTRFYIHVTPGIYREHIQIPSTK 80
           V++V  + IV ++  G+G++  I++A+ +AP   D +   F I+VT G+Y E++ I   K
Sbjct: 250 VNTVLVSDIVTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFVIYVTSGVYEENVVIAKNK 309

Query: 81  KFIALIGDNAFTTIIVDNRSNGTGFSTSDSATLEVKGNNFVAKYLSFENSAGSQNHRAVA 140
           +++ +IGD    T++  NR+   G++T +SAT  V   NFVA  ++F N+AG + H+AVA
Sbjct: 310 RYLMMIGDGINRTVVTGNRNVVDGWTTFNSATFAVTSPNFVAVNMTFRNTAGPEKHQAVA 369

Query: 141 VLDQADYTAYYKCRFLGFQDTLYLKGHNNFLKECDIYGTVDFIFGSGLVMFQDCNIYAR- 200
           +   AD + +Y C F  +QDTLY      F +ECDIYGTVDFIFG+  V+FQDCN+Y R 
Sbjct: 370 MRSSADLSIFYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQDCNLYPRQ 429

Query: 201 -FSKNEIVVTAQSKDQPMRKSGFSFQNCIVTVSSEIGVNEAKIKIYLGRPWRQYSSVVFM 260
                   +TAQ +  P + +G S  NC +  + ++  +   +K YLGRPW++YS  VFM
Sbjct: 430 PMQNQFNAITAQGRTDPNQNTGISIHNCTIKPADDLVSSNYTVKTYLGRPWKEYSRTVFM 489

Query: 261 ESFLDDNVQPKGWMFWQG-VPVNKLFYAEFNNRGPGANTKHRVHWSGFHVIRKKLAKKFT 320
           +S++D+ V+P GW  W G   ++ L+YAE+NN G G++T  RV W G+HVI    A  FT
Sbjct: 490 QSYIDEVVEPVGWREWNGDFALSTLYYAEYNNTGSGSSTTDRVVWPGYHVINSTDANNFT 549

Query: 321 VEKFINGTDWLPETGVPFKPG 334
           VE F+ G  W+ ++GVP+  G
Sbjct: 550 VENFLLGDGWMVQSGVPYISG 570

[Close]
BLAST of Cp4.1LG15g06250 vs. NCBI nr
Match: gi|659068777|ref|XP_008446175.1| (PREDICTED: pectinesterase 2-like [Cucumis melo])

HSP 1 Score: 451.8 bits (1161), Expect = 1.0e-123
Identity = 215/295 (72.88%), Postives = 243/295 (82.37%), Query Frame = 1 

Query: 41  ISDAIAAAPDNSKTRFYIHVTPGIYREHIQIPSTKKFIALIGDNAFTTIIVDNRSNGTGF 100
           I+ AIAAAPDNS TRFYI VTPG Y E +QIP TK FIALIGDNA TTIIVD+RSN  GF
Sbjct: 4   IAAAIAAAPDNSNTRFYIRVTPGTYHERLQIPPTKTFIALIGDNALTTIIVDDRSNARGF 63

Query: 101 STSDSATLEVKGNNFVAKYLSFENSAGSQNHRAVAVLDQADYTAYYKCRFLGFQDTLYLK 160
            TSDSATL V GNNF+AK L+FENSAG QN +AVAVLDQA +TAY+KC+FLGFQDTLY+K
Sbjct: 64  KTSDSATLTVNGNNFLAKSLTFENSAGPQNGQAVAVLDQAHFTAYHKCKFLGFQDTLYVK 123

Query: 161 GHNNFLKECDIYGTVDFIFGSGLVMFQDCNIYARFSKNEIVVTAQSKDQPMRKSGFSFQN 220
           G   F KEC+IYG+VDFIFG+GLVMFQDCNIYAR     I VTAQSK+QP   SGFSFQN
Sbjct: 124 G-TQFFKECEIYGSVDFIFGNGLVMFQDCNIYAREPNRPITVTAQSKEQPKEVSGFSFQN 183

Query: 221 CIVTVSSEIGVNEAKIKIYLGRPWRQYSSVVFMESFLDDNVQPKGWMFWQGVPVNKLFYA 280
           C VTVSSEI VN+A +KIYLGRPWRQYS VVFMESFLDD V P+GW  W GVPVN LFYA
Sbjct: 184 CTVTVSSEIAVNKANVKIYLGRPWRQYSKVVFMESFLDDEVVPEGWSEWPGVPVNNLFYA 243

Query: 281 EFNNRGPGANTKHRVHWSGFHVIRKKLAKKFTVEKFINGTDWLPETGVPFKPGFY 336
           EFNNRGPGA+   RVHW+GF V+ K+ A +FTV+ F+NG+ WLPETG+PF+ G +
Sbjct: 244 EFNNRGPGADVSKRVHWTGFRVLDKQSAYQFTVDNFVNGSKWLPETGIPFRDGLF 297

[Close]
BLAST of Cp4.1LG15g06250 vs. NCBI nr
Match: gi|778657958|ref|XP_004152582.2| (PREDICTED: pectinesterase 2-like [Cucumis sativus])

HSP 1 Score: 448.0 bits (1151), Expect = 1.5e-122
Identity = 213/335 (63.58%), Postives = 260/335 (77.61%), Query Frame = 1 

Query: 1   MASFSFVACFFVCFINFLACVDSVRFNAIVALDGSGHYKRISDAIAAAPDNSKTRFYIHV 60
           M  F  V C   CF N +  + S+ FN  V+LDGSG++KRISDAIAAAP+NS TRFYIHV
Sbjct: 1   MLFFFGVPCSLFCFFNLMLLIHSLSFNLTVSLDGSGNFKRISDAIAAAPNNSNTRFYIHV 60

Query: 61  TPGIYREHIQIPSTKKFIALIGDNAFTTIIVDNRSNGTGFSTSDSATLEVKGNNFVAKYL 120
            PG Y E +QIP  KKFIAL+GD+ FTTII+DNRSNGTGF T++SATL V+G+NF+A+ L
Sbjct: 61  APGTYHETLQIPRLKKFIALVGDDPFTTIIIDNRSNGTGFKTNNSATLTVQGDNFMAESL 120

Query: 121 SFENSAGSQNHRAVAVLDQADYTAYYKCRFLGFQDTLYLKGHNNFLKECDIYGTVDFIFG 180
           +FENSAG QN++AVAV D+A++TAYYKCRFL FQDTLY+ G   F KE DIYG+VDFI G
Sbjct: 121 TFENSAGPQNNQAVAVFDKANHTAYYKCRFLSFQDTLYVNGKPQFFKESDIYGSVDFICG 180

Query: 181 SGLVMFQDCNIYARFSKNEIVVTAQSKDQPMRKSGFSFQNCIVTVSSEIGVNEAKIKIYL 240
            G VMFQDCNIYAR   N I VTAQSK      SGFSFQNC VTVS EI  N+  +K++L
Sbjct: 181 YGQVMFQDCNIYARMPINSITVTAQSKYILRSVSGFSFQNCTVTVSREISSNKQNVKVFL 240

Query: 241 GRPWRQYSSVVFMESFLDDNVQPKGWMFWQGVPVNKLFYAEFNNRGPGANTKHRVHWSGF 300
           GRPW+QYS VVFMESFLDD V  +GW+ W GVPVN LFY EFNN GPGA+   RV+W+ +
Sbjct: 241 GRPWKQYSKVVFMESFLDDVVASEGWVEWIGVPVNNLFYGEFNNCGPGADVSKRVNWTSY 300

Query: 301 HVIRKKLAKKFTVEKFINGTDWLPETGVPFKPGFY 336
           H++ K+ A +FTV+ F+NG++WLPETG+PF+ G +
Sbjct: 301 HLLDKESALRFTVDNFVNGSEWLPETGIPFRRGLH 335

[Close]
BLAST of Cp4.1LG15g06250 vs. NCBI nr
Match: gi|659068775|ref|XP_008446163.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 39 [Cucumis melo])

HSP 1 Score: 445.3 bits (1144), Expect = 9.6e-122
Identity = 209/307 (68.08%), Postives = 244/307 (79.48%), Query Frame = 1 

Query: 29  IVALDGSGHYKRISDAIAAAPDNSKTRFYIHVTPGIYREHIQIPSTKKFIALIGDNAFTT 88
           +  + G      I+ AIAAAPDNS TRFYI VTPG Y E +QIP TK FIALIGDNA TT
Sbjct: 2   VKVIKGIDEQLTIAAAIAAAPDNSNTRFYIRVTPGTYHERLQIPPTKTFIALIGDNALTT 61

Query: 89  IIVDNRSNGTGFSTSDSATLEVKGNNFVAKYLSFENSAGSQNHRAVAVLDQADYTAYYKC 148
           IIVD+RSN  GF TSDSATL V G+NF+AK L+FENSAG QN +AVAVLDQA +TAY+KC
Sbjct: 62  IIVDDRSNARGFKTSDSATLTVNGDNFIAKSLTFENSAGPQNGQAVAVLDQARFTAYHKC 121

Query: 149 RFLGFQDTLYLKGHNNFLKECDIYGTVDFIFGSGLVMFQDCNIYARFSKNEIVVTAQSKD 208
           RFLGFQDTLY+ G ++F KECD+YGTVDFIFG+GL MFQDCNIYAR     I VTAQSK+
Sbjct: 122 RFLGFQDTLYVGGKSHFFKECDVYGTVDFIFGNGLTMFQDCNIYAREPNRPITVTAQSKE 181

Query: 209 QPMRKSGFSFQNCIVTVSSEIGVNEAKIKIYLGRPWRQYSSVVFMESFLDDNVQPKGWMF 268
           Q   +SGFSFQNC VTVSSEI  ++A +KIYL RPWRQYS VVFMESFLD+ V P+GW  
Sbjct: 182 QLHEESGFSFQNCTVTVSSEIVASKANVKIYLDRPWRQYSRVVFMESFLDEEVLPEGWSE 241

Query: 269 WQGVPVNKLFYAEFNNRGPGANTKHRVHWSGFHVIRKKLAKKFTVEKFINGTDWLPETGV 328
           W GVP N LFY EFNNRGPGA+   RVHW GFH++ K+LA +FT++ F+NG+ WLPETG+
Sbjct: 242 WPGVPTNNLFYGEFNNRGPGADVSKRVHWIGFHLLNKQLANQFTIDNFVNGSYWLPETGI 301

Query: 329 PFKPGFY 336
           PF+ GF+
Sbjct: 302 PFRRGFF 308

[Close]
BLAST of Cp4.1LG15g06250 vs. NCBI nr
Match: gi|778708428|ref|XP_011656190.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 41 [Cucumis sativus])

HSP 1 Score: 442.6 bits (1137), Expect = 6.2e-121
Identity = 208/304 (68.42%), Postives = 243/304 (79.93%), Query Frame = 1 

Query: 33  DGS-GHYKRISDAIAAAPDNSKTRFYIHVTPGIYREHIQIPSTKKFIALIGDNAFTTIIV 92
           DGS  H+K I+DAIA+ P+N  TRFYIHVTPG Y E +QIP TK FIALIGDNA TTIIV
Sbjct: 5   DGSIDHFKTITDAIASVPNNLNTRFYIHVTPGTYHECLQIPPTKTFIALIGDNALTTIIV 64

Query: 93  DNRSNGTGFSTSDSATLEVKGNNFVAKYLSFENSAGSQNHRAVAVLDQADYTAYYKCRFL 152
           D+RSN  GF T DSATL V GNNF+A+ L+F+NSAG QN +AVAVLD+A +T YYKCRFL
Sbjct: 65  DDRSNARGFKTIDSATLTVNGNNFLAQSLTFKNSAGPQNGQAVAVLDEAHFTTYYKCRFL 124

Query: 153 GFQDTLYLKGHNNFLKECDIYGTVDFIFGSGLVMFQDCNIYARFSKNEIVVTAQSKDQPM 212
           GFQDTLY++G   F KECDIYG+VDFIFG GLVMFQDCNIYAR     I +TAQSK Q  
Sbjct: 125 GFQDTLYVRGKPQFFKECDIYGSVDFIFGDGLVMFQDCNIYAREPNRSITITAQSKKQLH 184

Query: 213 RKSGFSFQNCIVTVSSEIGVNEAKIKIYLGRPWRQYSSVVFMESFLDDNVQPKGWMFWQG 272
            +SGFSFQNC +T+SSEI  N+A +KIYLGRPWRQYS VVFMESFLD  V PKGW+ W G
Sbjct: 185 EESGFSFQNCTITISSEIAANKADVKIYLGRPWRQYSQVVFMESFLDKEVMPKGWLKWSG 244

Query: 273 VPVNKLFYAEFNNRGPGANTKHRVHWSGFHVIRKKLAKKFTVEKFINGTDWLPETGVPFK 332
           VP+N LFY EFNNRGPGA+   R+H +GFHV+ K+ A +FT+  F+NG+DWLPETGVPF+
Sbjct: 245 VPLNNLFYGEFNNRGPGADVSKRIHCTGFHVLDKQSANQFTI-NFVNGSDWLPETGVPFR 304

Query: 333 PGFY 336
            G +
Sbjct: 305 SGLF 307

[Close]
BLAST of Cp4.1LG15g06250 vs. NCBI nr
Match: gi|659131731|ref|XP_008465826.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 39 [Cucumis melo])

HSP 1 Score: 402.5 bits (1033), Expect = 7.1e-109
Identity = 190/306 (62.09%), Postives = 238/306 (77.78%), Query Frame = 1 

Query: 30  VALDGSGHYKRISDAIAAAPDNSKTRFYIHVTPGIYREHIQIPSTKKFIALIGDNAFTTI 89
           ++LDG+G++ +++DAIAAAP+ S TRFYIHV PG Y+E I++PS K FIALIGD++ TTI
Sbjct: 19  LSLDGTGNFVKVNDAIAAAPNFSMTRFYIHVKPGTYQEIIEVPSEKTFIALIGDDSSTTI 78

Query: 90  IVDNRSNGTGFSTSDSATLEVKGNNFVAKYLSFENSAGSQNHRAVAVLDQADYTAYYKCR 149
           IV+NRSNGTG ST+ SATL V G NF+A++L+F+NSAG    +A+AVLDQA +TAYY+C 
Sbjct: 79  IVNNRSNGTGSSTASSATLTVYGENFMAQFLTFQNSAGPNEGQAIAVLDQAKHTAYYRCV 138

Query: 150 FLGFQDTLYLKGHNNFLKECDIYGTVDFIFGSGLVMFQDCNIYARFSKNEIVVTAQSKDQ 209
           FLG+QDTLY      F KECDIYG+VDFIFG+GLV+FQDCNIYAR    +I VTAQSK  
Sbjct: 139 FLGYQDTLYAGSVPQFFKECDIYGSVDFIFGNGLVVFQDCNIYARLFDVQITVTAQSKQS 198

Query: 210 PMRKSGFSFQNCIVTVSSEIGVNEAKIKIYLGRPWRQYSSVVFMESFLDDNVQPKGWMFW 269
               SGF FQNC VTVS EI  ++ K+ ++LGRPWR YS VVF++SFLD  VQPKGW+ W
Sbjct: 199 LSAPSGFIFQNCNVTVSPEIASSKDKVTVFLGRPWRMYSMVVFIDSFLDSVVQPKGWLEW 258

Query: 270 QGVPVNKLFYAEFNNRGPGANTKHRVHWSGFHVI-RKKLAKKFTVEKFINGTDWLPETGV 329
            GVP N L+YAE+NN G GANT  RV+W G+HV+   +    F+VE FINGT WLP+TG+
Sbjct: 259 PGVPENVLYYAEYNNSGGGANTSQRVNWPGYHVLDNAREVATFSVEMFINGTQWLPQTGI 318

Query: 330 PFKPGF 335
           PF+ GF
Sbjct: 319 PFRAGF 324

The following BLAST results are available for this feature:
BLAST of Cp4.1LG15g06250 vs. Swiss-Prot
Match NameE-valueIdentityDescription
PME2_CITSI3.6e-7644.23Pectinesterase 2 OS=Citrus sinensis GN=PECS-2.1 PE=2 SV=1[more]
PME7_ARATH2.3e-7543.55Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana GN=PM... [more]
PME40_ARATH1.2e-7443.95Probable pectinesterase/pectinesterase inhibitor 40 OS=Arabidopsis thaliana GN=P... [more]
PME39_ARATH4.4e-7443.44Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana GN=P... [more]
PME12_ARATH5.7e-7443.83Probable pectinesterase/pectinesterase inhibitor 12 OS=Arabidopsis thaliana GN=P... [more]
BLAST of Cp4.1LG15g06250 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LR14_CUCSA1.0e-12263.58Pectinesterase OS=Cucumis sativus GN=Csa_1G049450 PE=4 SV=1[more]
A0A0A0L1M3_CUCSA1.4e-10861.15Pectinesterase OS=Cucumis sativus GN=Csa_4G260430 PE=4 SV=1[more]
C0PT44_PICSI9.5e-8446.69Pectinesterase OS=Picea sitchensis PE=2 SV=1[more]
B0L3S3_TAICR6.8e-8245.60Pectinesterase OS=Taiwania cryptomerioides PE=2 SV=1[more]
V7BGJ7_PHAVU1.2e-8148.59Pectinesterase OS=Phaseolus vulgaris GN=PHAVU_007G198200g PE=4 SV=1[more]
BLAST of Cp4.1LG15g06250 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G02810.11.3e-7643.55 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT4G02320.16.5e-7643.95 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT4G02300.12.5e-7543.44 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT2G26440.13.2e-7543.83 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT4G02330.11.2e-7441.12 Plant invertase/pectin methylesterase inhibitor superfamily[more]
BLAST of Cp4.1LG15g06250 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|659068777|ref|XP_008446175.1|1.0e-12372.88PREDICTED: pectinesterase 2-like [Cucumis melo][more]
gi|778657958|ref|XP_004152582.2|1.5e-12263.58PREDICTED: pectinesterase 2-like [Cucumis sativus][more]
gi|659068775|ref|XP_008446163.1|9.6e-12268.08PREDICTED: probable pectinesterase/pectinesterase inhibitor 39 [Cucumis melo][more]
gi|778708428|ref|XP_011656190.1|6.2e-12168.42PREDICTED: probable pectinesterase/pectinesterase inhibitor 41 [Cucumis sativus][more]
gi|659131731|ref|XP_008465826.1|7.1e-10962.09PREDICTED: probable pectinesterase/pectinesterase inhibitor 39 [Cucumis melo][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0042545cell wall modification
Vocabulary: Molecular Function
TermDefinition
GO:0030599pectinesterase activity
Vocabulary: Cellular Component
TermDefinition
GO:0005618cell wall
Vocabulary: INTERPRO
TermDefinition
IPR012334Pectin_lyas_fold
IPR011050Pectin_lyase_fold/virulence
IPR000070Pectinesterase_cat
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0005982 starch metabolic process
biological_process GO:0005985 sucrose metabolic process
cellular_component GO:0005618 cell wall
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0045330 aspartyl esterase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG15g06250.1Cp4.1LG15g06250.1mRNA


InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 27..319
score: 1.1E
IPR011050Pectin lyase fold/virulence factorunknownSSF51126Pectin lyase-likecoord: 26..332
score: 5.58
IPR012334Pectin lyase foldGENE3DG3DSA:2.160.20.10coord: 25..332
score: 1.3E
NoneNo IPR availablePANTHERPTHR31707FAMILY NOT NAMEDcoord: 21..336
score: 3.8E

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Cp4.1LG15g06250CmaCh19G007750Cucurbita maxima (Rimu)cmacpeB522
Cp4.1LG15g06250CmoCh19G007860Cucurbita moschata (Rifu)cmocpeB481
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Cp4.1LG15g06250Melon (DHL92) v3.6.1cpemedB262
Cp4.1LG15g06250Silver-seed gourdcarcpeB0484
Cp4.1LG15g06250Silver-seed gourdcarcpeB1189
Cp4.1LG15g06250Silver-seed gourdcarcpeB1459
Cp4.1LG15g06250Cucumber (Chinese Long) v3cpecucB0310
Cp4.1LG15g06250Cucumber (Chinese Long) v3cpecucB0322
Cp4.1LG15g06250Wax gourdcpewgoB0326
Cp4.1LG15g06250Cucurbita pepo (Zucchini)cpecpeB280
Cp4.1LG15g06250Cucurbita pepo (Zucchini)cpecpeB255
Cp4.1LG15g06250Cucurbita pepo (Zucchini)cpecpeB267
Cp4.1LG15g06250Cucumber (Gy14) v1cgycpeB0206
Cp4.1LG15g06250Cucurbita maxima (Rimu)cmacpeB118
Cp4.1LG15g06250Cucurbita maxima (Rimu)cmacpeB560
Cp4.1LG15g06250Cucurbita maxima (Rimu)cmacpeB613
Cp4.1LG15g06250Cucurbita moschata (Rifu)cmocpeB093
Cp4.1LG15g06250Cucurbita moschata (Rifu)cmocpeB513
Cp4.1LG15g06250Cucurbita moschata (Rifu)cmocpeB562
Cp4.1LG15g06250Wild cucumber (PI 183967)cpecpiB259
Cp4.1LG15g06250Cucumber (Chinese Long) v2cpecuB257
Cp4.1LG15g06250Bottle gourd (USVL1VR-Ls)cpelsiB199
Cp4.1LG15g06250Watermelon (Charleston Gray)cpewcgB233
Cp4.1LG15g06250Watermelon (Charleston Gray)cpewcgB235
Cp4.1LG15g06250Watermelon (97103) v1cpewmB268
Cp4.1LG15g06250Melon (DHL92) v3.5.1cpemeB224
Cp4.1LG15g06250Cucumber (Gy14) v2cgybcpeB910