CmaCh19G007750 (gene) Cucurbita maxima (Rimu)

NameCmaCh19G007750
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionPectinesterase
LocationCma_Chr19 : 7593577 .. 7593951 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAAAAGAAGGATAGATTTTACAAGGTGAAGAGGTGAAAGGTGACAACTTTATGGCCCAACATTCAAGTTTTGAAAATTCTATCGAACATCAAAACGGTCAAGCAGTTGCTGTCTTTGACGATGCGGACTCTACTTTCTATTACAAGTGTAGGTTCCTTAATTTCCAAGACACCTTATATTCAAAGGGACGACCAGTTTTTTAAGGAGTGCGACATCTATGGAAATATAGACTTTATTTTTAGAGATGGACTTGTTACGTTTCAAGACTGTAACATATATATTCGGTTACCGAGCAATGAAACCACAGTCACGACGCAGTCCAAGAAACCTTTGTTGAAAAACTGCTTCTCATTACAAAACCAAATATCATAA

mRNA sequence

ATGAAAAAGAAGGATAGATTTTACAAGGTGACAACTTTATGGCCCAACATTCAAGTTTTGAAAATTCTATCGAACATCAAAACGGTCAAGCAGTTGCTGTCTTTGACGATGCGGACTCTACTTTCTATTACAAGTGTAGGTTCCTTAATTTCCAAGACACCTTATATTCAAAGGGACGACCAGTTTTTTAAGGAGTGCGACATCTATGGAAATATAGACTTTATTTTTAGAGATGGACTTGTTACGTTTCAAGACTGTAACATATATATTCGGTTACCGAGCAATGAAACCACAGTCACGACGCAGTCCAAGAAACCTTTGTTGAAAAACTGCTTCTCATTACAAAACCAAATATCATAA

Coding sequence (CDS)

ATGAAAAAGAAGGATAGATTTTACAAGGTGACAACTTTATGGCCCAACATTCAAGTTTTGAAAATTCTATCGAACATCAAAACGGTCAAGCAGTTGCTGTCTTTGACGATGCGGACTCTACTTTCTATTACAAGTGTAGGTTCCTTAATTTCCAAGACACCTTATATTCAAAGGGACGACCAGTTTTTTAAGGAGTGCGACATCTATGGAAATATAGACTTTATTTTTAGAGATGGACTTGTTACGTTTCAAGACTGTAACATATATATTCGGTTACCGAGCAATGAAACCACAGTCACGACGCAGTCCAAGAAACCTTTGTTGAAAAACTGCTTCTCATTACAAAACCAAATATCATAA

Protein sequence

MKKKDRFYKVTTLWPNIQVLKILSNIKTVKQLLSLTMRTLLSITSVGSLISKTPYIQRDDQFFKECDIYGNIDFIFRDGLVTFQDCNIYIRLPSNETTVTTQSKKPLLKNCFSLQNQIS
BLAST of CmaCh19G007750 vs. Swiss-Prot
Match: PME34_ARATH (Probable pectinesterase/pectinesterase inhibitor 34 OS=Arabidopsis thaliana GN=PME34 PE=2 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 3.3e-08
Identity = 26/55 (47.27%), Postives = 38/55 (69.09%), Query Frame = 1

Query: 53  TPYIQRDDQFFKECDIYGNIDFIFRDGLVTFQDCNIYIRLPSN--ETTVTTQSKK 106
           T Y+  + QFF+ECDIYG +DFIF +  V  Q+C+IY R P +  + T+T Q++K
Sbjct: 414 TLYVHSNRQFFRECDIYGTVDFIFGNAAVVLQNCSIYARKPMDFQKNTITAQNRK 468

BLAST of CmaCh19G007750 vs. Swiss-Prot
Match: PME2_CITSI (Pectinesterase 2 OS=Citrus sinensis GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 4.3e-08
Identity = 26/53 (49.06%), Postives = 35/53 (66.04%), Query Frame = 1

Query: 53  TPYIQRDDQFFKECDIYGNIDFIFRDGLVTFQDCNIYIRLPSNET-TVTTQSK 105
           T Y+    QF++ECDIYG +DFIF +  V  Q+CNI+ R P N T T+T Q +
Sbjct: 329 TLYVHSQRQFYRECDIYGTVDFIFGNAAVVLQNCNIFARKPPNRTNTLTAQGR 381

BLAST of CmaCh19G007750 vs. Swiss-Prot
Match: PME61_ARATH (Probable pectinesterase/pectinesterase inhibitor 61 OS=Arabidopsis thaliana GN=PME61 PE=2 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 9.6e-08
Identity = 25/53 (47.17%), Postives = 35/53 (66.04%), Query Frame = 1

Query: 55  YIQRDDQFFKECDIYGNIDFIFRDGLVTFQDCNIYIRLP--SNETTVTTQSKK 106
           Y+  + QFF+EC+IYG +DFIF +  V  Q CNIY R P    + T+T Q++K
Sbjct: 405 YVHSNRQFFRECEIYGTVDFIFGNAAVILQSCNIYARKPMAQQKITITAQNRK 457

BLAST of CmaCh19G007750 vs. Swiss-Prot
Match: PME40_ARATH (Probable pectinesterase/pectinesterase inhibitor 40 OS=Arabidopsis thaliana GN=PME40 PE=2 SV=1)

HSP 1 Score: 56.2 bits (134), Expect = 2.8e-07
Identity = 22/42 (52.38%), Postives = 30/42 (71.43%), Query Frame = 1

Query: 53  TPYIQRDDQFFKECDIYGNIDFIFRDGLVTFQDCNIYIRLPS 95
           T Y+    QF++ECDIYG +DFIF D  V FQ+C++Y R P+
Sbjct: 336 TIYVHSHKQFYRECDIYGTVDFIFGDASVVFQNCSLYARRPN 377

BLAST of CmaCh19G007750 vs. Swiss-Prot
Match: PME6_ARATH (Probable pectinesterase/pectinesterase inhibitor 6 OS=Arabidopsis thaliana GN=PME6 PE=2 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 4.8e-07
Identity = 25/55 (45.45%), Postives = 33/55 (60.00%), Query Frame = 1

Query: 53  TPYIQRDDQFFKECDIYGNIDFIFRDGLVTFQDCNIYIRLP--SNETTVTTQSKK 106
           T Y+    QF + C+IYG +DFIF D     Q+CNIY R P    + T+T QS+K
Sbjct: 381 TLYLHSSRQFLRNCNIYGTVDFIFGDATAILQNCNIYARKPMSGQKNTITAQSRK 435

BLAST of CmaCh19G007750 vs. TrEMBL
Match: A0A0A0L1M3_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_4G260430 PE=4 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 1.2e-09
Identity = 33/47 (70.21%), Postives = 37/47 (78.72%), Query Frame = 1

Query: 61  QFFKECDIYGNIDFIFRDGLVTFQDCNIYIRLPSNETTVTTQSKKPL 108
           QFFKECDIYG++DFIF +GLV FQDCNIY RL   + TVT QSK  L
Sbjct: 159 QFFKECDIYGSVDFIFGNGLVVFQDCNIYARLFDVQITVTAQSKPSL 205

BLAST of CmaCh19G007750 vs. TrEMBL
Match: A0A0A0LR14_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_1G049450 PE=4 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 4.6e-09
Identity = 37/65 (56.92%), Postives = 42/65 (64.62%), Query Frame = 1

Query: 53  TPYIQRDDQFFKECDIYGNIDFIFRDGLVTFQDCNIYIRLPSNETTVTTQSKKPLLK-NC 112
           T Y+    QFFKE DIYG++DFI   G V FQDCNIY R+P N  TVT QSK  L   + 
Sbjct: 156 TLYVNGKPQFFKESDIYGSVDFICGYGQVMFQDCNIYARMPINSITVTAQSKYILRSVSG 215

Query: 113 FSLQN 117
           FS QN
Sbjct: 216 FSFQN 220

BLAST of CmaCh19G007750 vs. TrEMBL
Match: A0A118JT55_CYNCS (Pectinesterase OS=Cynara cardunculus var. scolymus GN=Ccrd_008814 PE=4 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 6.7e-08
Identity = 28/53 (52.83%), Postives = 38/53 (71.70%), Query Frame = 1

Query: 53  TPYIQRDDQFFKECDIYGNIDFIFRDGLVTFQDCNIYIRLPSNET-TVTTQSK 105
           T Y+  D QF++ECD+YG +DFIF +  V FQ+CNIY R P N+T T+T Q +
Sbjct: 360 TLYVHSDRQFYRECDVYGTVDFIFGNAAVVFQNCNIYARKPPNKTNTLTAQGR 412

BLAST of CmaCh19G007750 vs. TrEMBL
Match: A0A0B0P3D3_GOSAR (Pectinesterase OS=Gossypium arboreum GN=F383_07269 PE=4 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 8.7e-08
Identity = 29/53 (54.72%), Postives = 38/53 (71.70%), Query Frame = 1

Query: 53  TPYIQRDDQFFKECDIYGNIDFIFRDGLVTFQDCNIYIRLPSNET-TVTTQSK 105
           T Y+  + QF++ECDIYG +D+IF +  V FQDCNIY R P N+T TVT Q +
Sbjct: 338 TLYVHSERQFYRECDIYGTVDWIFGNAAVVFQDCNIYARNPPNKTNTVTAQGR 390

BLAST of CmaCh19G007750 vs. TrEMBL
Match: A0A068V664_COFCA (Pectinesterase OS=Coffea canephora GN=GSCOC_T00016998001 PE=4 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 1.5e-07
Identity = 31/65 (47.69%), Postives = 39/65 (60.00%), Query Frame = 1

Query: 53  TPYIQRDDQFFKECDIYGNIDFIFRDGLVTFQDCNIYIRLPSNETTVTTQSKK-PLLKNC 112
           T Y +   QF+KECDIYG +DF+F D    FQ CN+Y  LP+   T T Q KK P   + 
Sbjct: 138 TLYAKSGKQFYKECDIYGTVDFVFGDAAAVFQSCNLYAWLPNRIITFTAQGKKIPNQVSG 197

Query: 113 FSLQN 117
           F +QN
Sbjct: 198 FVIQN 202

BLAST of CmaCh19G007750 vs. TAIR10
Match: AT3G49220.1 (AT3G49220.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 59.3 bits (142), Expect = 1.9e-09
Identity = 26/55 (47.27%), Postives = 38/55 (69.09%), Query Frame = 1

Query: 53  TPYIQRDDQFFKECDIYGNIDFIFRDGLVTFQDCNIYIRLPSN--ETTVTTQSKK 106
           T Y+  + QFF+ECDIYG +DFIF +  V  Q+C+IY R P +  + T+T Q++K
Sbjct: 414 TLYVHSNRQFFRECDIYGTVDFIFGNAAVVLQNCSIYARKPMDFQKNTITAQNRK 468

BLAST of CmaCh19G007750 vs. TAIR10
Match: AT5G53370.1 (AT5G53370.1 pectin methylesterase PCR fragment F)

HSP 1 Score: 57.8 bits (138), Expect = 5.4e-09
Identity = 25/53 (47.17%), Postives = 35/53 (66.04%), Query Frame = 1

Query: 55  YIQRDDQFFKECDIYGNIDFIFRDGLVTFQDCNIYIRLP--SNETTVTTQSKK 106
           Y+  + QFF+EC+IYG +DFIF +  V  Q CNIY R P    + T+T Q++K
Sbjct: 405 YVHSNRQFFRECEIYGTVDFIFGNAAVILQSCNIYARKPMAQQKITITAQNRK 457

BLAST of CmaCh19G007750 vs. TAIR10
Match: AT4G02320.1 (AT4G02320.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 56.2 bits (134), Expect = 1.6e-08
Identity = 22/42 (52.38%), Postives = 30/42 (71.43%), Query Frame = 1

Query: 53  TPYIQRDDQFFKECDIYGNIDFIFRDGLVTFQDCNIYIRLPS 95
           T Y+    QF++ECDIYG +DFIF D  V FQ+C++Y R P+
Sbjct: 336 TIYVHSHKQFYRECDIYGTVDFIFGDASVVFQNCSLYARRPN 377

BLAST of CmaCh19G007750 vs. TAIR10
Match: AT2G45220.1 (AT2G45220.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 55.5 bits (132), Expect = 2.7e-08
Identity = 22/53 (41.51%), Postives = 36/53 (67.92%), Query Frame = 1

Query: 53  TPYIQRDDQFFKECDIYGNIDFIFRDGLVTFQDCNIYIRLPSNET-TVTTQSK 105
           T Y+  + QF+++CD+YG +DFIF +     Q+CNI+ R P ++T T+T Q +
Sbjct: 331 TLYVHSNRQFYRDCDVYGTVDFIFGNAAAVLQNCNIFARRPRSKTNTITAQGR 383

BLAST of CmaCh19G007750 vs. TAIR10
Match: AT3G05620.1 (AT3G05620.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 55.5 bits (132), Expect = 2.7e-08
Identity = 29/67 (43.28%), Postives = 39/67 (58.21%), Query Frame = 1

Query: 53  TPYIQRDDQFFKECDIYGNIDFIFRDGLVTFQDCNIYIR--LPSNETTVTTQSKK-PLLK 112
           T Y     QF+++C+IYG IDFIF +G    Q+C IY R  LP  + T+T Q +K P   
Sbjct: 369 TLYAHSLRQFYRDCEIYGTIDFIFGNGAAVLQNCKIYTRVPLPLQKVTITAQGRKSPNQN 428

Query: 113 NCFSLQN 117
             F +QN
Sbjct: 429 TGFVIQN 435

BLAST of CmaCh19G007750 vs. NCBI nr
Match: gi|778708428|ref|XP_011656190.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 41 [Cucumis sativus])

HSP 1 Score: 82.4 bits (202), Expect = 5.8e-13
Identity = 40/65 (61.54%), Postives = 47/65 (72.31%), Query Frame = 1

Query: 53  TPYIQRDDQFFKECDIYGNIDFIFRDGLVTFQDCNIYIRLPSNETTVTTQSKKPLLKNC- 112
           T Y++   QFFKECDIYG++DFIF DGLV FQDCNIY R P+   T+T QSKK L +   
Sbjct: 129 TLYVRGKPQFFKECDIYGSVDFIFGDGLVMFQDCNIYAREPNRSITITAQSKKQLHEESG 188

Query: 113 FSLQN 117
           FS QN
Sbjct: 189 FSFQN 193

BLAST of CmaCh19G007750 vs. NCBI nr
Match: gi|659068775|ref|XP_008446163.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 39 [Cucumis melo])

HSP 1 Score: 73.9 bits (180), Expect = 2.1e-10
Identity = 36/65 (55.38%), Postives = 43/65 (66.15%), Query Frame = 1

Query: 53  TPYIQRDDQFFKECDIYGNIDFIFRDGLVTFQDCNIYIRLPSNETTVTTQSKKPLLKNC- 112
           T Y+     FFKECD+YG +DFIF +GL  FQDCNIY R P+   TVT QSK+ L +   
Sbjct: 129 TLYVGGKSHFFKECDVYGTVDFIFGNGLTMFQDCNIYAREPNRPITVTAQSKEQLHEESG 188

Query: 113 FSLQN 117
           FS QN
Sbjct: 189 FSFQN 193

BLAST of CmaCh19G007750 vs. NCBI nr
Match: gi|659068777|ref|XP_008446175.1| (PREDICTED: pectinesterase 2-like [Cucumis melo])

HSP 1 Score: 72.8 bits (177), Expect = 4.6e-10
Identity = 36/57 (63.16%), Postives = 43/57 (75.44%), Query Frame = 1

Query: 61  QFFKECDIYGNIDFIFRDGLVTFQDCNIYIRLPSNETTVTTQSK-KPLLKNCFSLQN 117
           QFFKEC+IYG++DFIF +GLV FQDCNIY R P+   TVT QSK +P   + FS QN
Sbjct: 126 QFFKECEIYGSVDFIFGNGLVMFQDCNIYAREPNRPITVTAQSKEQPKEVSGFSFQN 182

BLAST of CmaCh19G007750 vs. NCBI nr
Match: gi|659131264|ref|XP_008465596.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 47, partial [Cucumis melo])

HSP 1 Score: 72.4 bits (176), Expect = 6.0e-10
Identity = 38/65 (58.46%), Postives = 44/65 (67.69%), Query Frame = 1

Query: 53  TPYIQRDDQFFKECDIYGNIDFIFRDGLVTFQDCNIYIRLPSNETTVTTQSKKPLLK-NC 112
           T Y+    QFFKE +IYG++DFIF  G V FQDCNIY R+PSN  TVT QSK  L   + 
Sbjct: 95  TLYVNGKYQFFKESNIYGSVDFIFGHGRVMFQDCNIYARMPSNSITVTAQSKDFLRSISG 154

Query: 113 FSLQN 117
           FS QN
Sbjct: 155 FSFQN 159

BLAST of CmaCh19G007750 vs. NCBI nr
Match: gi|659131231|ref|XP_008465578.1| (PREDICTED: pectinesterase 2-like, partial [Cucumis melo])

HSP 1 Score: 72.4 bits (176), Expect = 6.0e-10
Identity = 38/65 (58.46%), Postives = 44/65 (67.69%), Query Frame = 1

Query: 53  TPYIQRDDQFFKECDIYGNIDFIFRDGLVTFQDCNIYIRLPSNETTVTTQSKKPLLK-NC 112
           T Y+    QFFKE +IYG++DFIF  G V FQDCNIY R+PSN  TVT QSK  L   + 
Sbjct: 47  TLYVNGKYQFFKESNIYGSVDFIFGHGRVMFQDCNIYARMPSNSITVTAQSKDFLRSISG 106

Query: 113 FSLQN 117
           FS QN
Sbjct: 107 FSFQN 111

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PME34_ARATH3.3e-0847.27Probable pectinesterase/pectinesterase inhibitor 34 OS=Arabidopsis thaliana GN=P... [more]
PME2_CITSI4.3e-0849.06Pectinesterase 2 OS=Citrus sinensis GN=PECS-2.1 PE=2 SV=1[more]
PME61_ARATH9.6e-0847.17Probable pectinesterase/pectinesterase inhibitor 61 OS=Arabidopsis thaliana GN=P... [more]
PME40_ARATH2.8e-0752.38Probable pectinesterase/pectinesterase inhibitor 40 OS=Arabidopsis thaliana GN=P... [more]
PME6_ARATH4.8e-0745.45Probable pectinesterase/pectinesterase inhibitor 6 OS=Arabidopsis thaliana GN=PM... [more]
Match NameE-valueIdentityDescription
A0A0A0L1M3_CUCSA1.2e-0970.21Pectinesterase OS=Cucumis sativus GN=Csa_4G260430 PE=4 SV=1[more]
A0A0A0LR14_CUCSA4.6e-0956.92Pectinesterase OS=Cucumis sativus GN=Csa_1G049450 PE=4 SV=1[more]
A0A118JT55_CYNCS6.7e-0852.83Pectinesterase OS=Cynara cardunculus var. scolymus GN=Ccrd_008814 PE=4 SV=1[more]
A0A0B0P3D3_GOSAR8.7e-0854.72Pectinesterase OS=Gossypium arboreum GN=F383_07269 PE=4 SV=1[more]
A0A068V664_COFCA1.5e-0747.69Pectinesterase OS=Coffea canephora GN=GSCOC_T00016998001 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G49220.11.9e-0947.27 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT5G53370.15.4e-0947.17 pectin methylesterase PCR fragment F[more]
AT4G02320.11.6e-0852.38 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT2G45220.12.7e-0841.51 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT3G05620.12.7e-0843.28 Plant invertase/pectin methylesterase inhibitor superfamily[more]
Match NameE-valueIdentityDescription
gi|778708428|ref|XP_011656190.1|5.8e-1361.54PREDICTED: probable pectinesterase/pectinesterase inhibitor 41 [Cucumis sativus][more]
gi|659068775|ref|XP_008446163.1|2.1e-1055.38PREDICTED: probable pectinesterase/pectinesterase inhibitor 39 [Cucumis melo][more]
gi|659068777|ref|XP_008446175.1|4.6e-1063.16PREDICTED: pectinesterase 2-like [Cucumis melo][more]
gi|659131264|ref|XP_008465596.1|6.0e-1058.46PREDICTED: probable pectinesterase/pectinesterase inhibitor 47, partial [Cucumis... [more]
gi|659131231|ref|XP_008465578.1|6.0e-1058.46PREDICTED: pectinesterase 2-like, partial [Cucumis melo][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000070Pectinesterase_cat
IPR011050Pectin_lyase_fold/virulence
IPR012334Pectin_lyas_fold
Vocabulary: Cellular Component
TermDefinition
GO:0005618cell wall
Vocabulary: Molecular Function
TermDefinition
GO:0030599pectinesterase activity
Vocabulary: Biological Process
TermDefinition
GO:0042545cell wall modification
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0005982 starch metabolic process
biological_process GO:0005985 sucrose metabolic process
cellular_component GO:0005618 cell wall
molecular_function GO:0030599 pectinesterase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh19G007750.1CmaCh19G007750.1mRNA


Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 53..107
score: 6.5
IPR011050Pectin lyase fold/virulence factorunknownSSF51126Pectin lyase-likecoord: 55..98
score: 2.3
IPR012334Pectin lyase foldGENE3DG3DSA:2.160.20.10coord: 52..106
score: 2.9
NoneNo IPR availablePANTHERPTHR31707FAMILY NOT NAMEDcoord: 53..105
score: 1.8
NoneNo IPR availablePANTHERPTHR31707:SF27PECTINESTERASE/PECTINESTERASE INHIBITOR 6-RELATEDcoord: 53..105
score: 1.8

The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
CmaCh19G007750CmoCh19G007860Cucurbita moschata (Rifu)cmacmoB508
CmaCh19G007750Cp4.1LG15g06250Cucurbita pepo (Zucchini)cmacpeB522
The following gene(s) are paralogous to this gene:

None