Cp4.1LG13g00150 (gene) Cucurbita pepo (Zucchini)

NameCp4.1LG13g00150
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
DescriptionMethylenetetrahydrofolate reductase
LocationCp4.1LG13 : 122702 .. 128017 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AGAGATGGATGAGGTTTAAAAGATGTTCACCGACAGCTGCCAGAGGTGATTGAGGTGATTTCCTATCTTCCTTCTCATTCTCTGTTTCACTGATCTCTCAATCTCTCAATCTCCTCTTTTCTCTCTCTTCCTCACTCTTCAATTTCAATTCTCCCCATATCATTCACATTGCTCATTTCCTCACTCTTCAATTTCAATTCTCCCCATATCATTCACATTGCTGATTTCCTCACCAACACACACGCACCCACCCTTGCTGCTTCCGATCTGTAGCTTCTACGCCACTACAGGGAAGAGGAGATGAAGGTGATTGACAAGATCACGGCAGCCACCGCCGGTGATGGCGGCAGAGTTGTGTTCTCCTTTGAGTACTTCCCGCCCAAGACGGAGGACGGTGTTGATAACCTCTTCGACAGGATGGACCGGATGGTGGCTCATAATCCTTCCTTCTGTGACATTACTTGGGGCGCCGGTGGCTCCACCGCCGATCTCACCCTCGACATTGCTAACAAGATGCAGAACATGATCTGTGTCGAGACCATGATGCATCTTACCTGCACCAATATGCCTGTTGAGAAGATCGACCATGCTCTTCAGACTATTAAGTCTAATGGCCTTCAGAATGTCCTCGCGCTTCGCGGCGACCCTCCTCATGGACAGGATAAATTCGTCCAGATCGAAGGCGGCTTCGCTTGTGCTCTCGATCTTGTACTCTACAATGGCTTCGTCTCTCTTCGTTCTCTCTGTTCTGTGTTTCTGCTCTCTAAATGTTTCCATTTCTTATTCGTAGGTTAAGCACATCAGGGAGAAGTATGGTGACTACTTCGGCATTACTGTAGCTGGATATCCAGGTTTCTTTTTTGTGTTTTGATCGAAAGTTACAGATCTACTTGCAAATGCCTTTTCTTTTCTGTAACTAACGGAATGCTAATGCTTAATTCTATTCTATTCTATTCTATTCTTCTCAGAGGCACATCCGGACGTGATTGGAAGCAATGGCGTGGCCACGACTGAAGGCTACCAGAGCGATCTGGCTTATCTTAAGAAAAAGGTGAATCTGATCTTTAAGCAATTTAATTCCTCTTCCCCTGTTCTTACATGGATTACATTTTATATGCTATTTTGTCTGTTCTTCTCTTTCTTTTTCGATTGCTTTTATTTACTATATTGTCTTTGCTTCTTTTCAGTTTGATGCCGGGGCTGATCTGATTGTTACCCAACTCTTCTATGACACTGACATTTTCCTCAAGTTTGTTAACGATTGTCGTCAAATTGGAATTACTTGTCCGATTGTACCTGGTATTATGCCCATCAATAATTACAAAGGTTTCTTACGCATGACCAGCTTTTGCAAAACGAAGGTACTAACCTCTCTCTAATCTTTTGATTAATGGCCTTGATTTAAATTAAATGCGTCCACTCTAGAAAAATTTACGACCAGCGATCATCAAAATTTTGCCCTTTCTTCTCCAAAGAAATATTAATATTATCTGTAATGTTAAATTGTTTCTCTTTTACCTGTATGTATGACTTTCGAAAAATTGCAGATTCCGGATGAGGTCATGGCTGCCTTAGAGCCAATCAAGGATAACGAAGAAGCTGTCAAAGCTTATGGAATTCACCTTGGAACTGAAATGTGCAAGAAGATTTTGGCTCATGGAATCAAGACTCTTCACATGTATACACTGAATATGGAGAAATCGGCATTGGCTATACTAATGGTATATTCCAGTATCCTAAACGTATTCTATCTTTACTTCCAATTATTGTTTCTATGGGTAATTTTGTCATTGGATNTTTTTTTTTTTTTTTTTTTTTTAATCTTTTTTGGAGGCTTGTAGTTTAATCTGTTTTTTTTGGGGTTAAGTCTTAAATGCATTTCTCTGCTCATGGCAGAATCTTGGTCTGATTGAAGAGAGCAAAGTCTCAAGATCATTACCTTGGAGGCGCCCTACAAATGTTTTCCGTATCAGGGAAGATGTTCGCCCAATCTTCTGGTATCTCTTATGCTTATTGCAAAATGAGGATGCATTCCTAATGATTTTGGATGCATAAAATTGTTTTACATTTTGCTAAATTTGGGATAGAGCTGATCTAGGTAGTTGTTGATCACAGGGCTAATCGTCCTAGAAGCTACATATCAAGGACCATAGGTTGGGAGCAGTACCCTCATGGTAGATGGAGTGATTCTAGTAATCCTTCATATGGGGCACTGACTGACTATCAGGTATGAACAATAAACTAGAACTTGTAAATGATCTATGTTGATATTTGTTACATACACACCTTCTAGAGAGATAGAGAGACAGAGACAGTTGTACGGAGGAGATTTCCTTTTAACATCTGGAATTATTTCTTGTGGACTGCCTGAAAACTTAGTTAACTCTTTGTGATCTGCATGAATTCGAGACTTAAAAGTAGGCTTTGTGAAATTTCCCGCATGATGTATCATTTACTTTGGCTTGGTCTGATTCATCTGACACGTCTCAAATCCTGTTGAAAGCTTCTGGTAGAATTTCCCCCAGATAATTTGTTTGTTTCTTTAGTTATTATAGGCAAGCGGGTGAGATGATTTCTTTTTCATGTTTCTCAAACGCTTCAAAGAGCTGAAAATTTGAACTTTACTTCATTCAATATTTATTTGAATATTGTTTTAGCACTTTTAACATAGTTTCTAGTGTGTTTAATCAGTGTTACTTTTGCTCTGTTTTGCACTTAAGTTTATGCGTCCACGTGCACGTGACAAGAAGCTTCTTGAGGAATGGGCTCTCTCATTGAAAAGCTTAGAGGATGTTTATGAGGTACTTTTCCTAACCAAGAGTGTGAAGAAAGTTTGTTATAATGTTAGCGATTGACCAGATATGTTATACTGTTTCATATCAGAAATTTATGAAGTACTGCCTTGGAAAGTTGAGAAGCAGTCCGTGGTCAGAATTAGACGGTCTTCAGGCTGAGACAAGAATAATAAGCGAACAGCTTGGTAAAATTAACATGAAAGGTTTCCTTTCAATCAACAGCCAACCAGCGGTTAATGGAGAAAGATCTGATTCTCCTTCTGTTGGTAAGTACAAATTAATCCATTCATACAATTAACTTTTTTGACAGCATAACTGAGTAATTATTTGAAAGTTTTGCAATACAGTCTCAGAGGTTAGTGGACAATGCTCTCTTCTTATCATTTCAAGAGTGGACGATTTCATCTTTGTTCGCCCTATAACTGTTGAAGTCAAAGGAAAAGTACTGAAATACTAAAACTGAACTAGATAATTTTTTCTCATGTAGCAAATTCATATTTGGCTAAAAAAACTAAATAAATAAAAACAATATATTACTGGCTCACCAATTGATAAATTTTAGTTTATTACATTATTTTATAAATAATGTTTGATTATTAATTATTAATATACTAATTCATAGGACCCTTTGACTGGCTAGTAAATTCATAAGTTAATGTATGAAGAATAATGAATGGTTTAAATTCTATTTTGTTCCTTGTAATTTCATGTTTGGTTCACAGGTCCCTGAACTTTTAATTAAGTGTTTTAGTTCTCAATTTCAATTATTCCGTTTTAATCCTTGAACATCCTTCTTTTTTTTTTTTCTTTTTTTTTTTTTGTTGTTGTTGTTGTCGTCTTTATTGGGGACTTGCTTCAAAACCAAAGCAAGTTAAATATGAAGAAATCATGCTATCAACATGCATCGAGTGATCTAATATAGAATTTCTTGGAACCTTCCCATAGACTTTCAGTAACTATAATTTTGTATCAGGGATTGAGGGAGAATTTAATATGCCCAAGGGAATGGAGCCCACTTCAGTTTCCATTCATATTCCCTTGAAAATCAGAAACCATTGTTGGACCTATCTCTCAAATACTACTAATGGTTATTTGAAGAGATGAAAAACGTGTTGACAGTATCCTACTAAAAGTGAATTCTTTGGCCTTTACCCACTTTGGAACTAGTTGGAAATTAATATGTAGTGGAACACGTTGTTCCTCTAATTGAACAACTAAGCATGATACCTTGATAGAAGTGTAAATTTTTACCATAAATAGATAACACCCTAACAAAACAATCATTTGTATGCAAGGGATCAGAGTCGCCTGTCTAGAGAGGGGGGGTATCTTCCCCTGTGCATCTTTTGTTTATTTGTTTAGTTTTGTAGTGATCAATTCAGTTTTGACTTATGATATTTGTGGCAATGGCGGCAGAATGCTTGTTTAGTCATCTGATCAGTGTTGGGATGATTAATAATGATTTGCAGGATGGGGTGGATCAGGAGGTTACGTTTATCAAAAGGCATACATCGAGTTTTTCTGTTCCAGGGAAAACTTGGATGCTGTTGTTGACAAATGCAGGGGCCTTCCATCTCTAACCTACATGGCAGTGAATAAGGAAGGCATTTGGGTATCCAACATCACTCAAAATGATGTGAATGCTGTAACATGGGGAGTTTTCCCAGCCAAAGAGATCGTCCAGCCAACTGTCGTCGATCCTGCCAGCTTTCTAGTTTGGAAAGATGAGGCATTTGAGATTTGGTCAAGGGGATGGGCTTCCTTGTACCCTGAGGGCGACCCGTCCCGAAAATTTCTCGAAGAGGTTGGTATACTTGTTATATCTTTTCAGTGCTCAATTAAATTCCCCGTTCTTTTTTCACCTGATATCACCCCTTGGTAGAGAAGTGTGGATTATTAAGTGATGGAAATGCTTTGAATCGAAATTTATGTTGTCATCGAAAGGCTTAATCAGGAAATGCTGTTTTATCTATAAAAGGTTAATCTTAATATGGTGATGGAAGCTAAGAAATTTTGTGGAAGATTACTATCTCGATGACAAAGCACTTTATTTGTTTCAGATACAGAGCAGCTATTACTTGGTGAGCTTGGTGGATAACGATTACGTCAATTCTAACCTCTTTGCTGCTTTGGAGGGATTCTGAGGTTGAGCTGCTATTCCTTTCCATGATTCTAGTTTTACTTCCATCTTTCGATCTCTCATTGAAACCTTGCCCGAGGTTAATGCAAGAAGAATTTTCTTTTTCCGTTTGCTTGAGTTTAATAATATGATTCCCAATAAGGGCTGTCCATTTATTAAGAACATTGGGATTTTAAAACTTCCCGAGGTTGAAGGTAATGTGTCATTCATTTGTGACTAAAAACATTAGATTAAAATATTTCTTAAGGTAGCCATAGCCTTGGGCATGGGATACTATATTATATGGTTTGTAGAATTTTCAATTTTTAACGTGTTTATCATTGGAAATTTACTTGTTTTTTGTTTTTTTATAGCACTCGTTTATTTTAATGGAAATTGGTT

mRNA sequence

AGAGATGGATGAGGTTTAAAAGATGTTCACCGACAGCTGCCAGAGGTGATTGAGGTGATTTCCTATCTTCCTTCTCATTCTCTGTTTCACTGATCTCTCAATCTCTCAATCTCCTCTTTTCTCTCTCTTCCTCACTCTTCAATTTCAATTCTCCCCATATCATTCACATTGCTCATTTCCTCACTCTTCAATTTCAATTCTCCCCATATCATTCACATTGCTGATTTCCTCACCAACACACACGCACCCACCCTTGCTGCTTCCGATCTGTAGCTTCTACGCCACTACAGGGAAGAGGAGATGAAGGTGATTGACAAGATCACGGCAGCCACCGCCGGTGATGGCGGCAGAGTTGTGTTCTCCTTTGAGTACTTCCCGCCCAAGACGGAGGACGGTGTTGATAACCTCTTCGACAGGATGGACCGGATGGTGGCTCATAATCCTTCCTTCTGTGACATTACTTGGGGCGCCGGTGGCTCCACCGCCGATCTCACCCTCGACATTGCTAACAAGATGCAGAACATGATCTGTGTCGAGACCATGATGCATCTTACCTGCACCAATATGCCTGTTGAGAAGATCGACCATGCTCTTCAGACTATTAAGTCTAATGGCCTTCAGAATGTCCTCGCGCTTCGCGGCGACCCTCCTCATGGACAGGATAAATTCGTCCAGATCGAAGGCGGCTTCGCTTGTGCTCTCGATCTTGTTAAGCACATCAGGGAGAAGTATGGTGACTACTTCGGCATTACTGTAGCTGGATATCCAGAGGCACATCCGGACGTGATTGGAAGCAATGGCGTGGCCACGACTGAAGGCTACCAGAGCGATCTGGCTTATCTTAAGAAAAAGTTTGATGCCGGGGCTGATCTGATTGTTACCCAACTCTTCTATGACACTGACATTTTCCTCAAGTTTGTTAACGATTGTCGTCAAATTGGAATTACTTGTCCGATTGTACCTGGTATTATGCCCATCAATAATTACAAAGGTTTCTTACGCATGACCAGCTTTTGCAAAACGAAGATTCCGGATGAGGTCATGGCTGCCTTAGAGCCAATCAAGGATAACGAAGAAGCTGTCAAAGCTTATGGAATTCACCTTGGAACTGAAATGTGCAAGAAGATTTTGGCTCATGGAATCAAGACTCTTCACATGTATACACTGAATATGGAGAAATCGGCATTGGCTATACTAATGAATCTTGGTCTGATTGAAGAGAGCAAAGTCTCAAGATCATTACCTTGGAGGCGCCCTACAAATGTTTTCCGTATCAGGGAAGATGTTCGCCCAATCTTCTGGGCTAATCGTCCTAGAAGCTACATATCAAGGACCATAGGTTGGGAGCAGTACCCTCATGGTAGATGGAGTGATTCTAGTAATCCTTCATATGGGGCACTGACTGACTATCAGTTTATGCGTCCACGTGCACGTGACAAGAAGCTTCTTGAGGAATGGGCTCTCTCATTGAAAAGCTTAGAGGATGTTTATGAGAAATTTATGAAGTACTGCCTTGGAAAGTTGAGAAGCAGTCCGTGGTCAGAATTAGACGGTCTTCAGGCTGAGACAAGAATAATAAGCGAACAGCTTGGTAAAATTAACATGAAAGGTTTCCTTTCAATCAACAGCCAACCAGCGGTTAATGGAGAAAGATCTGATTCTCCTTCTGTTGGATGGGGTGGATCAGGAGGTTACGTTTATCAAAAGGCATACATCGAGTTTTTCTGTTCCAGGGAAAACTTGGATGCTGTTGTTGACAAATGCAGGGGCCTTCCATCTCTAACCTACATGGCAGTGAATAAGGAAGGCATTTGGGTATCCAACATCACTCAAAATGATGTGAATGCTGTAACATGGGGAGTTTTCCCAGCCAAAGAGATCGTCCAGCCAACTGTCGTCGATCCTGCCAGCTTTCTAGTTTGGAAAGATGAGGCATTTGAGATTTGGTCAAGGGGATGGGCTTCCTTGTACCCTGAGGGCGACCCGTCCCGAAAATTTCTCGAAGAGATACAGAGCAGCTATTACTTGGTGAGCTTGGTGGATAACGATTACGTCAATTCTAACCTCTTTGCTGCTTTGGAGGGATTCTGAGGTTGAGCTGCTATTCCTTTCCATGATTCTAGTTTTACTTCCATCTTTCGATCTCTCATTGAAACCTTGCCCGAGGTTAATGCAAGAAGAATTTTCTTTTTCCGTTTGCTTGAGTTTAATAATATGATTCCCAATAAGGGCTGTTGAAGGTAATGTGTCATTCATTTGTGACTAAAAACATTAGATTAAAATATTTCTTAAGGTAGCCATAGCCTTGGGCATGGGATACTATATTATATGGTTTGTAGAATTTTCAATTTTTAACGTGTTTATCATTGGAAATTTACTTGTTTTTTGTTTTTTTATAGCACTCGTTTATTTTAATGGAAATTGGTT

Coding sequence (CDS)

ATGAAGGTGATTGACAAGATCACGGCAGCCACCGCCGGTGATGGCGGCAGAGTTGTGTTCTCCTTTGAGTACTTCCCGCCCAAGACGGAGGACGGTGTTGATAACCTCTTCGACAGGATGGACCGGATGGTGGCTCATAATCCTTCCTTCTGTGACATTACTTGGGGCGCCGGTGGCTCCACCGCCGATCTCACCCTCGACATTGCTAACAAGATGCAGAACATGATCTGTGTCGAGACCATGATGCATCTTACCTGCACCAATATGCCTGTTGAGAAGATCGACCATGCTCTTCAGACTATTAAGTCTAATGGCCTTCAGAATGTCCTCGCGCTTCGCGGCGACCCTCCTCATGGACAGGATAAATTCGTCCAGATCGAAGGCGGCTTCGCTTGTGCTCTCGATCTTGTTAAGCACATCAGGGAGAAGTATGGTGACTACTTCGGCATTACTGTAGCTGGATATCCAGAGGCACATCCGGACGTGATTGGAAGCAATGGCGTGGCCACGACTGAAGGCTACCAGAGCGATCTGGCTTATCTTAAGAAAAAGTTTGATGCCGGGGCTGATCTGATTGTTACCCAACTCTTCTATGACACTGACATTTTCCTCAAGTTTGTTAACGATTGTCGTCAAATTGGAATTACTTGTCCGATTGTACCTGGTATTATGCCCATCAATAATTACAAAGGTTTCTTACGCATGACCAGCTTTTGCAAAACGAAGATTCCGGATGAGGTCATGGCTGCCTTAGAGCCAATCAAGGATAACGAAGAAGCTGTCAAAGCTTATGGAATTCACCTTGGAACTGAAATGTGCAAGAAGATTTTGGCTCATGGAATCAAGACTCTTCACATGTATACACTGAATATGGAGAAATCGGCATTGGCTATACTAATGAATCTTGGTCTGATTGAAGAGAGCAAAGTCTCAAGATCATTACCTTGGAGGCGCCCTACAAATGTTTTCCGTATCAGGGAAGATGTTCGCCCAATCTTCTGGGCTAATCGTCCTAGAAGCTACATATCAAGGACCATAGGTTGGGAGCAGTACCCTCATGGTAGATGGAGTGATTCTAGTAATCCTTCATATGGGGCACTGACTGACTATCAGTTTATGCGTCCACGTGCACGTGACAAGAAGCTTCTTGAGGAATGGGCTCTCTCATTGAAAAGCTTAGAGGATGTTTATGAGAAATTTATGAAGTACTGCCTTGGAAAGTTGAGAAGCAGTCCGTGGTCAGAATTAGACGGTCTTCAGGCTGAGACAAGAATAATAAGCGAACAGCTTGGTAAAATTAACATGAAAGGTTTCCTTTCAATCAACAGCCAACCAGCGGTTAATGGAGAAAGATCTGATTCTCCTTCTGTTGGATGGGGTGGATCAGGAGGTTACGTTTATCAAAAGGCATACATCGAGTTTTTCTGTTCCAGGGAAAACTTGGATGCTGTTGTTGACAAATGCAGGGGCCTTCCATCTCTAACCTACATGGCAGTGAATAAGGAAGGCATTTGGGTATCCAACATCACTCAAAATGATGTGAATGCTGTAACATGGGGAGTTTTCCCAGCCAAAGAGATCGTCCAGCCAACTGTCGTCGATCCTGCCAGCTTTCTAGTTTGGAAAGATGAGGCATTTGAGATTTGGTCAAGGGGATGGGCTTCCTTGTACCCTGAGGGCGACCCGTCCCGAAAATTTCTCGAAGAGATACAGAGCAGCTATTACTTGGTGAGCTTGGTGGATAACGATTACGTCAATTCTAACCTCTTTGCTGCTTTGGAGGGATTCTGA

Protein sequence

MKVIDKITAATAGDGGRVVFSFEYFPPKTEDGVDNLFDRMDRMVAHNPSFCDITWGAGGSTADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGLQNVLALRGDPPHGQDKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATTEGYQSDLAYLKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTSFCKTKIPDEVMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHMYTLNMEKSALAILMNLGLIEESKVSRSLPWRRPTNVFRIREDVRPIFWANRPRSYISRTIGWEQYPHGRWSDSSNPSYGALTDYQFMRPRARDKKLLEEWALSLKSLEDVYEKFMKYCLGKLRSSPWSELDGLQAETRIISEQLGKINMKGFLSINSQPAVNGERSDSPSVGWGGSGGYVYQKAYIEFFCSRENLDAVVDKCRGLPSLTYMAVNKEGIWVSNITQNDVNAVTWGVFPAKEIVQPTVVDPASFLVWKDEAFEIWSRGWASLYPEGDPSRKFLEEIQSSYYLVSLVDNDYVNSNLFAALEGF
BLAST of Cp4.1LG13g00150 vs. Swiss-Prot
Match: MTHR2_ARATH (Methylenetetrahydrofolate reductase 2 OS=Arabidopsis thaliana GN=MTHFR2 PE=1 SV=2)

HSP 1 Score: 1030.0 bits (2662), Expect = 1.0e-299
Identity = 477/591 (80.71%), Postives = 535/591 (90.52%), Query Frame = 1

Query: 1   MKVIDKITAATAGDGGRVVFSFEYFPPKTEDGVDNLFDRMDRMVAHNPSFCDITWGAGGS 60
           MKVIDKI +    D G+  FSFE+FPPKTEDGVDNLF+RMDRMVA+ P+FCDITWGAGGS
Sbjct: 1   MKVIDKIQSLA--DEGKTAFSFEFFPPKTEDGVDNLFERMDRMVAYGPTFCDITWGAGGS 60

Query: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGLQNVLALRGDPPHGQ 120
           TADLTLDIA++MQN++CVE+MMHLTCTNMPVEKIDHAL+TI+SNG+QNVLALRGDPPHGQ
Sbjct: 61  TADLTLDIASRMQNVVCVESMMHLTCTNMPVEKIDHALETIRSNGIQNVLALRGDPPHGQ 120

Query: 121 DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATTEGYQSDLAY 180
           DKFVQ+EGGF CALDLV HIR KYGDYFGITVAGYPEAHPDVIG NG+A+ E YQSDL Y
Sbjct: 121 DKFVQVEGGFDCALDLVNHIRSKYGDYFGITVAGYPEAHPDVIGENGLASNEAYQSDLEY 180

Query: 181 LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTSFCK 240
           LKKK DAGADLIVTQLFYDTDIFLKFVNDCRQIGI+CPIVPGIMPINNY+GFLRMT FCK
Sbjct: 181 LKKKIDAGADLIVTQLFYDTDIFLKFVNDCRQIGISCPIVPGIMPINNYRGFLRMTGFCK 240

Query: 241 TKIPDEVMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHMYTLNMEKSALAILM 300
           TKIP EVMAALEPIKDNEEAVKAYGIHLGTEMCKK+LAHG+K+LH+YTLNMEKSALAILM
Sbjct: 241 TKIPVEVMAALEPIKDNEEAVKAYGIHLGTEMCKKMLAHGVKSLHLYTLNMEKSALAILM 300

Query: 301 NLGLIEESKVSRSLPWRRPTNVFRIREDVRPIFWANRPRSYISRTIGWEQYPHGRWSDSS 360
           NLG+I+ESK+SRSLPWRRP NVFR +EDVRPIFWANRP+SYISRT GWE +P GRW DS 
Sbjct: 301 NLGMIDESKISRSLPWRRPANVFRTKEDVRPIFWANRPKSYISRTKGWEDFPQGRWGDSR 360

Query: 361 NPSYGALTDYQFMRPRARDKKLLEEWALSLKSLEDVYEKFMKYCLGKLRSSPWSELDGLQ 420
           + SYGAL+D+QF RPRARDKKL +EW + LKS+ED+ EKF + CLG L+SSPWSELDGLQ
Sbjct: 361 SASYGALSDHQFSRPRARDKKLQQEWVVPLKSVEDIQEKFKELCLGNLKSSPWSELDGLQ 420

Query: 421 AETRIISEQLGKINMKGFLSINSQPAVNGERSDSPSVGWGGSGGYVYQKAYIEFFCSREN 480
            ETRII+EQL K+N KGFL+INSQP+VN ERSDSP+VGWGG  GYVYQKAY+EFFCS+E 
Sbjct: 421 PETRIINEQLIKVNSKGFLTINSQPSVNAERSDSPTVGWGGPVGYVYQKAYLEFFCSKEK 480

Query: 481 LDAVVDKCRGLPSLTYMAVNKEGIWVSNITQNDVNAVTWGVFPAKEIVQPTVVDPASFLV 540
           LDAVV+KC+ LPS+TYMAVNK   WVSN  Q DVNAVTWGVFPAKEI+QPT+VDPASF V
Sbjct: 481 LDAVVEKCKALPSITYMAVNKGEQWVSNTAQADVNAVTWGVFPAKEIIQPTIVDPASFNV 540

Query: 541 WKDEAFEIWSRGWASLYPEGDPSRKFLEEIQSSYYLVSLVDNDYVNSNLFA 592
           WKDEAFE WSR WA+LYPE DPSR  LEE+++SYYLVSLV+NDY+N ++FA
Sbjct: 541 WKDEAFETWSRSWANLYPEADPSRNLLEEVKNSYYLVSLVENDYINGDIFA 589

BLAST of Cp4.1LG13g00150 vs. Swiss-Prot
Match: MTHR_ORYSJ (Probable methylenetetrahydrofolate reductase OS=Oryza sativa subsp. japonica GN=Os03g0815200 PE=2 SV=1)

HSP 1 Score: 1025.4 bits (2650), Expect = 2.5e-298
Identity = 481/594 (80.98%), Postives = 531/594 (89.39%), Query Frame = 1

Query: 1   MKVIDKITAATAGDGGRVVFSFEYFPPKTEDGVDNLFDRMDRMVAHNPSFCDITWGAGGS 60
           MKVI+KI  A A   GR VFSFEYFPPKTE+G+DNLF+RMDRMVAH P+FCDITWGAGGS
Sbjct: 1   MKVIEKIQEAAAD--GRTVFSFEYFPPKTEEGLDNLFERMDRMVAHGPNFCDITWGAGGS 60

Query: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGLQNVLALRGDPPHGQ 120
           TADLTL+IAN+MQNM+CVETMMHLTCTNMPVEKID AL TIKSNG+QNVLALRGDPPHGQ
Sbjct: 61  TADLTLEIANRMQNMVCVETMMHLTCTNMPVEKIDDALTTIKSNGIQNVLALRGDPPHGQ 120

Query: 121 DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATTEGYQSDLAY 180
           DKFVQ+ GGFACALDLV+HIR KYGDYFGITVAGYPEAHPD I S   AT E Y +DLAY
Sbjct: 121 DKFVQVAGGFACALDLVQHIRAKYGDYFGITVAGYPEAHPDAIQSTEGATPEAYSNDLAY 180

Query: 181 LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTSFCK 240
           LK+K DAGADLI+TQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMT FCK
Sbjct: 181 LKQKVDAGADLIITQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCK 240

Query: 241 TKIPDEVMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHMYTLNMEKSALAILM 300
           TKIP E+ AALEPIKDNEEAVKAYGIHLGTEMCKKILA GIKTLH+YTLNMEKSAL ILM
Sbjct: 241 TKIPAEITAALEPIKDNEEAVKAYGIHLGTEMCKKILATGIKTLHLYTLNMEKSALGILM 300

Query: 301 NLGLIEESKVSRSLPWRRPTNVFRIREDVRPIFWANRPRSYISRTIGWEQYPHGRWSDSS 360
           NLGLIEESK+SRSLPWR PTNVFR++EDVRPIFWANRP+SYISRT+GW+QYPHGRW DS 
Sbjct: 301 NLGLIEESKISRSLPWRPPTNVFRVKEDVRPIFWANRPKSYISRTLGWDQYPHGRWGDSR 360

Query: 361 NPSYGALTDYQFMRPRARDKKLLEEWALSLKSLEDVYEKFMKYCLGKLRSSPWSELDGLQ 420
           NPSYGALTDYQF RPR R KKL EEWA+ +KS+ED+ E+FM +C GKL SSPWSELDGLQ
Sbjct: 361 NPSYGALTDYQFTRPRGRGKKLQEEWAVPVKSVEDINERFMNFCQGKLTSSPWSELDGLQ 420

Query: 421 AETRIISEQLGKINMKGFLSINSQPAVNGERSDSPSVGWGGSGGYVYQKAYIEFFCSREN 480
            ET+II +QL KIN KGFL+INSQPAVNGERSDS SVGWGG GGYVYQKAY+EFFCS+E 
Sbjct: 421 PETKIIDDQLVKINQKGFLTINSQPAVNGERSDSTSVGWGGPGGYVYQKAYLEFFCSKEK 480

Query: 481 LDAVVDKCRGLPSLTYMAVNKEGIWVSNITQNDVNAVTWGVFPAKEIVQPTVVDPASFLV 540
           LD +++K +  PSLTY+AVNK+G   SNI  N VNAVTWGVFP KEIVQPTVVD ASF+V
Sbjct: 481 LDQLIEKSKAFPSLTYIAVNKDGESFSNIPTNAVNAVTWGVFPGKEIVQPTVVDSASFMV 540

Query: 541 WKDEAFEIWSRGWASLYPEGDPSRKFLEEIQSSYYLVSLVDNDYVNSNLFAALE 595
           WKDEAFEIWS+GWA L+PEGD SR+ L+++Q SY+LVSLVDNDY+N +LFAA +
Sbjct: 541 WKDEAFEIWSKGWACLFPEGDSSREILDKVQKSYFLVSLVDNDYINGDLFAAFK 592

BLAST of Cp4.1LG13g00150 vs. Swiss-Prot
Match: MTHR1_MAIZE (Methylenetetrahydrofolate reductase 1 OS=Zea mays PE=2 SV=1)

HSP 1 Score: 1014.2 bits (2621), Expect = 5.8e-295
Identity = 472/594 (79.46%), Postives = 529/594 (89.06%), Query Frame = 1

Query: 1   MKVIDKITAATAGDGGRVVFSFEYFPPKTEDGVDNLFDRMDRMVAHNPSFCDITWGAGGS 60
           MKVI+KI  A AGDG R  FSFEYFPPKTE+GV+NLF+RMDRMVAH PSFCDITWGAGGS
Sbjct: 1   MKVIEKILEA-AGDG-RTAFSFEYFPPKTEEGVENLFERMDRMVAHGPSFCDITWGAGGS 60

Query: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGLQNVLALRGDPPHGQ 120
           TADLTL+IAN+MQNM+CVETMMHLTCTNMPVEKIDHAL+TIKSNG+QNVLALRGDPPHGQ
Sbjct: 61  TADLTLEIANRMQNMVCVETMMHLTCTNMPVEKIDHALETIKSNGIQNVLALRGDPPHGQ 120

Query: 121 DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATTEGYQSDLAY 180
           DKFVQ+EGGFACALDLV+HIR KYGDYFGITVAGYPEAHPD I   G AT E Y +DLAY
Sbjct: 121 DKFVQVEGGFACALDLVQHIRAKYGDYFGITVAGYPEAHPDAIQGEGGATLEAYSNDLAY 180

Query: 181 LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTSFCK 240
           LK+K DAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMT FCK
Sbjct: 181 LKRKVDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCK 240

Query: 241 TKIPDEVMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHMYTLNMEKSALAILM 300
           TKIP E+ AAL+PIKDNEEAV+ YGIHLGTEMCKKILA GIKTLH+YTLNM+KSA+ ILM
Sbjct: 241 TKIPSEITAALDPIKDNEEAVRQYGIHLGTEMCKKILATGIKTLHLYTLNMDKSAIGILM 300

Query: 301 NLGLIEESKVSRSLPWRRPTNVFRIREDVRPIFWANRPRSYISRTIGWEQYPHGRWSDSS 360
           NLGLIEESKVSR LPWR  TNVFR++EDVRPIFWANRP+SY+ RT+GW+QYPHGRW DS 
Sbjct: 301 NLGLIEESKVSRPLPWRPATNVFRVKEDVRPIFWANRPKSYLKRTLGWDQYPHGRWGDSR 360

Query: 361 NPSYGALTDYQFMRPRARDKKLLEEWALSLKSLEDVYEKFMKYCLGKLRSSPWSELDGLQ 420
           NPSYGALTD+QF RPR R KKL EEWA+ LKS+ED+ E+F  +C GKL SSPWSELDGLQ
Sbjct: 361 NPSYGALTDHQFTRPRGRGKKLQEEWAVPLKSVEDISERFTNFCQGKLTSSPWSELDGLQ 420

Query: 421 AETRIISEQLGKINMKGFLSINSQPAVNGERSDSPSVGWGGSGGYVYQKAYIEFFCSREN 480
            ET+II +QL  IN KGFL+INSQPAVNGE+SDSP+VGWGG GGYVYQKAY+EFFC++E 
Sbjct: 421 PETKIIDDQLVNINQKGFLTINSQPAVNGEKSDSPTVGWGGPGGYVYQKAYLEFFCAKEK 480

Query: 481 LDAVVDKCRGLPSLTYMAVNKEGIWVSNITQNDVNAVTWGVFPAKEIVQPTVVDPASFLV 540
           LD +++K +  PSLTY+AVNK+G   SNI+ N VNAVTWGVFP KEI+QPTVVD ASF+V
Sbjct: 481 LDQLIEKIKAFPSLTYIAVNKDGETFSNISPNAVNAVTWGVFPGKEIIQPTVVDHASFMV 540

Query: 541 WKDEAFEIWSRGWASLYPEGDPSRKFLEEIQSSYYLVSLVDNDYVNSNLFAALE 595
           WKDEAFEIW+RGW  ++PEGD SR+ LE++Q +YYLVSLVDNDYV  +LFAA +
Sbjct: 541 WKDEAFEIWTRGWGCMFPEGDSSRELLEKVQKTYYLVSLVDNDYVQGDLFAAFK 592

BLAST of Cp4.1LG13g00150 vs. Swiss-Prot
Match: MTHR1_ARATH (Methylenetetrahydrofolate reductase 1 OS=Arabidopsis thaliana GN=MTHFR1 PE=1 SV=1)

HSP 1 Score: 999.2 bits (2582), Expect = 1.9e-290
Identity = 454/591 (76.82%), Postives = 528/591 (89.34%), Query Frame = 1

Query: 1   MKVIDKITAATAGDGGRVVFSFEYFPPKTEDGVDNLFDRMDRMVAHNPSFCDITWGAGGS 60
           MKV+DKI + T  + G+  FSFE+FPPKTEDGV+NLF+RMDR+V++ P+FCDITWGAGGS
Sbjct: 1   MKVVDKIKSVT--EQGQTAFSFEFFPPKTEDGVENLFERMDRLVSYGPTFCDITWGAGGS 60

Query: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGLQNVLALRGDPPHGQ 120
           TADLTL+IA++MQN+ICVETMMHLTCTNMP+EKIDHAL+TI+SNG+QNVLALRGDPPHGQ
Sbjct: 61  TADLTLEIASRMQNVICVETMMHLTCTNMPIEKIDHALETIRSNGIQNVLALRGDPPHGQ 120

Query: 121 DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATTEGYQSDLAY 180
           DKFVQ+EGGFACALDLV HIR KYGDYFGITVAGYPEAHPDVI ++G+AT E YQSDLAY
Sbjct: 121 DKFVQVEGGFACALDLVNHIRSKYGDYFGITVAGYPEAHPDVIEADGLATPESYQSDLAY 180

Query: 181 LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTSFCK 240
           LKKK DAGADLIVTQLFYDTDIFLKFVNDCRQIGI CPIVPGIMPI+NYKGFLRM  FCK
Sbjct: 181 LKKKVDAGADLIVTQLFYDTDIFLKFVNDCRQIGINCPIVPGIMPISNYKGFLRMAGFCK 240

Query: 241 TKIPDEVMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHMYTLNMEKSALAILM 300
           TKIP E+ AALEPIKDN+EAVKAYGIH  TEMCKKILAHGI +LH+YTLN++KSA+ ILM
Sbjct: 241 TKIPAELTAALEPIKDNDEAVKAYGIHFATEMCKKILAHGITSLHLYTLNVDKSAIGILM 300

Query: 301 NLGLIEESKVSRSLPWRRPTNVFRIREDVRPIFWANRPRSYISRTIGWEQYPHGRWSDSS 360
           NLGLI+ESK+SRSLPWRRP NVFR +EDVRPIFWANRP+SYISRT GW  +PHGRW DS 
Sbjct: 301 NLGLIDESKISRSLPWRRPANVFRTKEDVRPIFWANRPKSYISRTKGWNDFPHGRWGDSH 360

Query: 361 NPSYGALTDYQFMRPRARDKKLLEEWALSLKSLEDVYEKFMKYCLGKLRSSPWSELDGLQ 420
           + +Y  L+DYQF RP+ RDKKL +EW + LKS+EDV EKF + C+G L+SSPWSELDGLQ
Sbjct: 361 SAAYSTLSDYQFARPKGRDKKLQQEWVVPLKSIEDVQEKFKELCIGNLKSSPWSELDGLQ 420

Query: 421 AETRIISEQLGKINMKGFLSINSQPAVNGERSDSPSVGWGGSGGYVYQKAYIEFFCSREN 480
            ET+II+EQLGKIN  GFL+INSQP+VN  +SDSP++GWGG GGYVYQKAY+EFFCS++ 
Sbjct: 421 PETKIINEQLGKINSNGFLTINSQPSVNAAKSDSPAIGWGGPGGYVYQKAYLEFFCSKDK 480

Query: 481 LDAVVDKCRGLPSLTYMAVNKEGIWVSNITQNDVNAVTWGVFPAKEIVQPTVVDPASFLV 540
           LD +V+K +  PS+TYMAVNK   WVSN  ++DVNAVTWGVFPAKE++QPT+VDPASF V
Sbjct: 481 LDTLVEKSKAFPSITYMAVNKSENWVSNTGESDVNAVTWGVFPAKEVIQPTIVDPASFKV 540

Query: 541 WKDEAFEIWSRGWASLYPEGDPSRKFLEEIQSSYYLVSLVDNDYVNSNLFA 592
           WKDEAFEIWSR WA+LYPE DPSRK LEE+++SYYLVSLVDN+Y+N ++F+
Sbjct: 541 WKDEAFEIWSRSWANLYPEDDPSRKLLEEVKNSYYLVSLVDNNYINGDIFS 589

BLAST of Cp4.1LG13g00150 vs. Swiss-Prot
Match: MTHR_HUMAN (Methylenetetrahydrofolate reductase OS=Homo sapiens GN=MTHFR PE=1 SV=3)

HSP 1 Score: 529.6 bits (1363), Expect = 4.3e-149
Identity = 263/590 (44.58%), Postives = 372/590 (63.05%), Query Frame = 1

Query: 14  DGGRVVFSFEYFPPKTEDGVDNLFDRMDRMVAHNPSFCDITWGAGGSTAD----LTLDIA 73
           + G   FS E+FPP+T +G  NL  R DRM A  P + D+TW   G         ++ IA
Sbjct: 54  ESGDKWFSLEFFPPRTAEGAVNLISRFDRMAAGGPLYIDVTWHPAGDPGSDKETSSMMIA 113

Query: 74  NKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGLQNVLALRGDPPHGQDKFVQIEGG 133
           +   N   +ET++H+TC    +E+I   L   K  GL+N++ALRGDP    D++ + EGG
Sbjct: 114 STAVNYCGLETILHMTCCRQRLEEITGHLHKAKQLGLKNIMALRGDPIG--DQWEEEEGG 173

Query: 134 FACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATTEGYQSDLAYLKKKFDAGA 193
           F  A+DLVKHIR ++GDYF I VAGYP+ HP+  GS        +++DL +LK+K  AGA
Sbjct: 174 FNYAVDLVKHIRSEFGDYFDICVAGYPKGHPEA-GS--------FEADLKHLKEKVSAGA 233

Query: 194 DLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTSFCKTKIPDEVMA 253
           D I+TQLF++ D F +FV  C  +GITCPIVPGI PI  Y    ++    K ++P E+  
Sbjct: 234 DFIITQLFFEADTFFRFVKACTDMGITCPIVPGIFPIQGYHSLRQLVKLSKLEVPQEIKD 293

Query: 254 ALEPIKDNEEAVKAYGIHLGTEMCKKILAHG-IKTLHMYTLNMEKSALAILMNLGLIEES 313
            +EPIKDN+ A++ YGI L   +C+++LA G +  LH YTLN E +   +L  LG+  E 
Sbjct: 294 VIEPIKDNDAAIRNYGIELAVSLCQELLASGLVPGLHFYTLNREMATTEVLKRLGMWTED 353

Query: 314 KVSRSLPWRRPTNVFRIREDVRPIFWANRPRSYISRTIGWEQYPHGRWSDSSNPSYGALT 373
              R LPW    +  R  EDVRPIFWA+RP+SYI RT  W+++P+GRW +SS+P++G L 
Sbjct: 354 P-RRPLPWALSAHPKRREEDVRPIFWASRPKSYIYRTQEWDEFPNGRWGNSSSPAFGELK 413

Query: 374 DYQ--FMRPRARDKKLLEEWALSLKSLEDVYEKFMKYCLG-------KLRSSPWSELDGL 433
           DY   +++ ++  ++LL+ W   L S E V+E F+ Y  G       K+   PW++ + L
Sbjct: 414 DYYLFYLKSKSPKEELLKMWGEELTSEESVFEVFVLYLSGEPNRNGHKVTCLPWND-EPL 473

Query: 434 QAETRIISEQLGKINMKGFLSINSQPAVNGERSDSPSVGWGGSGGYVYQKAYIEFFCSRE 493
            AET ++ E+L ++N +G L+INSQP +NG+ S  P VGWG SGGYV+QKAY+EFF SRE
Sbjct: 474 AAETSLLKEELLRVNRQGILTINSQPNINGKPSSDPIVGWGPSGGYVFQKAYLEFFTSRE 533

Query: 494 NLDAVVDKCRGLP-SLTYMAVNKEGIWVSNITQNDVNAVTWGVFPAKEIVQPTVVDPASF 553
             +A++   +     + Y  VN +G  ++N  +   NAVTWG+FP +EI+QPTVVDP SF
Sbjct: 534 TAEALLQVLKKYELRVNYHLVNVKGENITNAPELQPNAVTWGIFPGREIIQPTVVDPVSF 593

Query: 554 LVWKDEAFEIWSRGWASLYPEGDPSRKFLEEIQSSYYLVSLVDNDYVNSN 589
           + WKDEAF +W   W  LY E  PSR  ++ I  +Y+LV+LVDND+   N
Sbjct: 594 MFWKDEAFALWIERWGKLYEEESPSRTIIQYIHDNYFLVNLVDNDFPLDN 630

BLAST of Cp4.1LG13g00150 vs. TrEMBL
Match: A0A0A0LIC6_CUCSA (Methylenetetrahydrofolate reductase OS=Cucumis sativus GN=Csa_2G149470 PE=3 SV=1)

HSP 1 Score: 1190.6 bits (3079), Expect = 0.0e+00
Identity = 566/596 (94.97%), Postives = 581/596 (97.48%), Query Frame = 1

Query: 1   MKVIDKITAATAGDGGRVVFSFEYFPPKTEDGVDNLFDRMDRMVAHNPSFCDITWGAGGS 60
           MKVIDKI AATAGDGGRVVFSFEYFPPKTEDGVDNLF+RMDRMVAHNPSFCDITWGAGGS
Sbjct: 43  MKVIDKIRAATAGDGGRVVFSFEYFPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGGS 102

Query: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGLQNVLALRGDPPHGQ 120
           TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGLQNVLALRGDPPHGQ
Sbjct: 103 TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGLQNVLALRGDPPHGQ 162

Query: 121 DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATTEGYQSDLAY 180
           DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATTEGYQSDLAY
Sbjct: 163 DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATTEGYQSDLAY 222

Query: 181 LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTSFCK 240
           LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMT FCK
Sbjct: 223 LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCK 282

Query: 241 TKIPDEVMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHMYTLNMEKSALAILM 300
           TKIPDE+MAALEPIKDNEEAVKAYGIHLGTEMCKKIL HGIKTLH+YTLNMEKSALAILM
Sbjct: 283 TKIPDEIMAALEPIKDNEEAVKAYGIHLGTEMCKKILTHGIKTLHIYTLNMEKSALAILM 342

Query: 301 NLGLIEESKVSRSLPWRRPTNVFRIREDVRPIFWANRPRSYISRTIGWEQYPHGRWSDSS 360
           NLGLIEESKVSRSLPWRRPTNVFR+REDVRPIFWANRPRSYISRTIGW+QYPHGRWSDS 
Sbjct: 343 NLGLIEESKVSRSLPWRRPTNVFRVREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDSR 402

Query: 361 NPSYGALTDYQFMRPRARDKKLLEEWALSLKSLEDVYEKFMKYCLGKLRSSPWSELDGLQ 420
           NPSYGALTDYQFMRPRARDKKLLEEWAL LKS+EDVYEKFMKYCLGKLRSSPWSEL+GLQ
Sbjct: 403 NPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELEGLQ 462

Query: 421 AETRIISEQLGKINMKGFLSINSQPAVNGERSDSPSVGWGGSGGYVYQKAYIEFFCSREN 480
           AETR+ISEQLGKINMKGFLSINSQPAVNGERSDSP VGWGG GGYVYQKAYIEFFCSRE 
Sbjct: 463 AETRMISEQLGKINMKGFLSINSQPAVNGERSDSPCVGWGGPGGYVYQKAYIEFFCSREK 522

Query: 481 LDAVVDKCRGLPSLTYMAVNKEGIWVSNITQNDVNAVTWGVFPAKEIVQPTVVDPASFLV 540
           LDA+VDKCR LPSLTYMAVNKEGIWVSN TQ DVNAVTWGVFPAKEI+QPTVVDPASF+V
Sbjct: 523 LDAIVDKCRALPSLTYMAVNKEGIWVSNATQKDVNAVTWGVFPAKEIIQPTVVDPASFIV 582

Query: 541 WKDEAFEIWSRGWASLYPEGDPSRKFLEEIQSSYYLVSLVDNDYVNSNLFAALEGF 597
           WKDEAFEIWSRGWA+LYPEGD SRK L+EIQSSYYLVSLVDNDY+NS+LFA LE F
Sbjct: 583 WKDEAFEIWSRGWAALYPEGDWSRKLLDEIQSSYYLVSLVDNDYINSDLFAILEDF 638

BLAST of Cp4.1LG13g00150 vs. TrEMBL
Match: I1KG02_SOYBN (Methylenetetrahydrofolate reductase OS=Glycine max GN=GLYMA_06G325300 PE=3 SV=1)

HSP 1 Score: 1080.1 bits (2792), Expect = 0.0e+00
Identity = 502/596 (84.23%), Postives = 551/596 (92.45%), Query Frame = 1

Query: 1   MKVIDKITAATAGDGGRVVFSFEYFPPKTEDGVDNLFDRMDRMVAHNPSFCDITWGAGGS 60
           MK+I+KI AA+A D  RVVFSFE+FPPKTEDGVDNLF+RMDRMV HNPSFCDITWGAGG+
Sbjct: 1   MKIIEKIHAASA-DPNRVVFSFEFFPPKTEDGVDNLFERMDRMVVHNPSFCDITWGAGGT 60

Query: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGLQNVLALRGDPPHGQ 120
           TADLTL+IANKMQN++CVETMMHLTCTNMPVEKIDHAL TIKSNGLQNVLALRGDPPHGQ
Sbjct: 61  TADLTLEIANKMQNIVCVETMMHLTCTNMPVEKIDHALHTIKSNGLQNVLALRGDPPHGQ 120

Query: 121 DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATTEGYQSDLAY 180
           DKFVQ+EGGFACA DLV+HIR KYGDYFGITVAGYPEAHPDVI S+G+AT+EGYQ+DLAY
Sbjct: 121 DKFVQVEGGFACARDLVQHIRAKYGDYFGITVAGYPEAHPDVIESDGLATSEGYQNDLAY 180

Query: 181 LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTSFCK 240
           LK K DAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGF+RMT FCK
Sbjct: 181 LKSKVDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFIRMTGFCK 240

Query: 241 TKIPDEVMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHMYTLNMEKSALAILM 300
           TKIP ++MAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGI TLH+YTLNMEKSALAILM
Sbjct: 241 TKIPADIMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGINTLHLYTLNMEKSALAILM 300

Query: 301 NLGLIEESKVSRSLPWRRPTNVFRIREDVRPIFWANRPRSYISRTIGWEQYPHGRWSDSS 360
           NLGLIEE+KVSRSLPWRRP NVFR++EDVRPIFWANRP+SYISRTIGW+QYPHGRW DS 
Sbjct: 301 NLGLIEETKVSRSLPWRRPANVFRVKEDVRPIFWANRPKSYISRTIGWDQYPHGRWGDSC 360

Query: 361 NPSYGALTDYQFMRPRARDKKLLEEWALSLKSLEDVYEKFMKYCLGKLRSSPWSELDGLQ 420
           NPSYGAL+DYQFMRPRARDKKL+EEWA+ LKS+ED+YE+F  YCLGKLRS+PWSELDGLQ
Sbjct: 361 NPSYGALSDYQFMRPRARDKKLVEEWAVPLKSIEDIYERFRLYCLGKLRSNPWSELDGLQ 420

Query: 421 AETRIISEQLGKINMKGFLSINSQPAVNGERSDSPSVGWGGSGGYVYQKAYIEFFCSREN 480
            ET+II+E L KIN KGFL+INSQPAVNGE+SDSP+VGWGG GGYVYQKAY+EFFCS+E 
Sbjct: 421 PETKIINELLEKINTKGFLTINSQPAVNGEKSDSPTVGWGGPGGYVYQKAYVEFFCSKEK 480

Query: 481 LDAVVDKCRGLPSLTYMAVNKEGIWVSNITQNDVNAVTWGVFPAKEIVQPTVVDPASFLV 540
           LDA+VDKC+   SLTYMAVNK+G W SN+ Q DVNAVTWGVFPAKEI+QPT+VDP SF V
Sbjct: 481 LDALVDKCKDRTSLTYMAVNKDGSWKSNVGQTDVNAVTWGVFPAKEIIQPTIVDPVSFNV 540

Query: 541 WKDEAFEIWSRGWASLYPEGDPSRKFLEEIQSSYYLVSLVDNDYVNSNLFAALEGF 597
           WKDEAFEIWSRGWASLYPEGD SRK +EE+  SY+LVSLVDNDY+N +LF     F
Sbjct: 541 WKDEAFEIWSRGWASLYPEGDASRKLVEEVGGSYFLVSLVDNDYINGDLFTVFADF 595

BLAST of Cp4.1LG13g00150 vs. TrEMBL
Match: A0A0B2QJZ4_GLYSO (Methylenetetrahydrofolate reductase OS=Glycine soja GN=glysoja_033227 PE=3 SV=1)

HSP 1 Score: 1080.1 bits (2792), Expect = 0.0e+00
Identity = 502/596 (84.23%), Postives = 551/596 (92.45%), Query Frame = 1

Query: 1   MKVIDKITAATAGDGGRVVFSFEYFPPKTEDGVDNLFDRMDRMVAHNPSFCDITWGAGGS 60
           MK+I+KI AA+A D  RVVFSFE+FPPKTEDGVDNLF+RMDRMV HNPSFCDITWGAGG+
Sbjct: 1   MKIIEKIHAASA-DPNRVVFSFEFFPPKTEDGVDNLFERMDRMVVHNPSFCDITWGAGGT 60

Query: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGLQNVLALRGDPPHGQ 120
           TADLTL+IANKMQN++CVETMMHLTCTNMPVEKIDHAL TIKSNGLQNVLALRGDPPHGQ
Sbjct: 61  TADLTLEIANKMQNIVCVETMMHLTCTNMPVEKIDHALHTIKSNGLQNVLALRGDPPHGQ 120

Query: 121 DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATTEGYQSDLAY 180
           DKFVQ+EGGFACA DLV+HIR KYGDYFGITVAGYPEAHPDVI S+G+AT+EGYQ+DLAY
Sbjct: 121 DKFVQVEGGFACARDLVQHIRAKYGDYFGITVAGYPEAHPDVIESDGLATSEGYQNDLAY 180

Query: 181 LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTSFCK 240
           LK K DAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGF+RMT FCK
Sbjct: 181 LKSKVDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFIRMTGFCK 240

Query: 241 TKIPDEVMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHMYTLNMEKSALAILM 300
           TKIP ++MAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGI TLH+YTLNMEKSALAILM
Sbjct: 241 TKIPADIMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGINTLHLYTLNMEKSALAILM 300

Query: 301 NLGLIEESKVSRSLPWRRPTNVFRIREDVRPIFWANRPRSYISRTIGWEQYPHGRWSDSS 360
           NLGLIEE+KVSRSLPWRRP NVFR++EDVRPIFWANRP+SYISRTIGW+QYPHGRW DS 
Sbjct: 301 NLGLIEETKVSRSLPWRRPANVFRVKEDVRPIFWANRPKSYISRTIGWDQYPHGRWGDSC 360

Query: 361 NPSYGALTDYQFMRPRARDKKLLEEWALSLKSLEDVYEKFMKYCLGKLRSSPWSELDGLQ 420
           NPSYGAL+DYQFMRPRARDKKL+EEWA+ LKS+ED+YE+F  YCLGKLRS+PWSELDGLQ
Sbjct: 361 NPSYGALSDYQFMRPRARDKKLVEEWAVPLKSIEDIYERFRLYCLGKLRSNPWSELDGLQ 420

Query: 421 AETRIISEQLGKINMKGFLSINSQPAVNGERSDSPSVGWGGSGGYVYQKAYIEFFCSREN 480
            ET+II+E L KIN KGFL+INSQPAVNGE+SDSP+VGWGG GGYVYQKAY+EFFCS+E 
Sbjct: 421 PETKIINELLEKINTKGFLTINSQPAVNGEKSDSPTVGWGGPGGYVYQKAYVEFFCSKEK 480

Query: 481 LDAVVDKCRGLPSLTYMAVNKEGIWVSNITQNDVNAVTWGVFPAKEIVQPTVVDPASFLV 540
           LDA+VDKC+   SLTYMAVNK+G W SN+ Q DVNAVTWGVFPAKEI+QPT+VDP SF V
Sbjct: 481 LDALVDKCKDRTSLTYMAVNKDGSWKSNVGQTDVNAVTWGVFPAKEIIQPTIVDPVSFNV 540

Query: 541 WKDEAFEIWSRGWASLYPEGDPSRKFLEEIQSSYYLVSLVDNDYVNSNLFAALEGF 597
           WKDEAFEIWSRGWASLYPEGD SRK +EE+  SY+LVSLVDNDY+N +LF     F
Sbjct: 541 WKDEAFEIWSRGWASLYPEGDASRKLVEEVGGSYFLVSLVDNDYINGDLFTVFADF 595

BLAST of Cp4.1LG13g00150 vs. TrEMBL
Match: I1JZE2_SOYBN (Methylenetetrahydrofolate reductase OS=Glycine max GN=GLYMA_04G256100 PE=3 SV=1)

HSP 1 Score: 1080.1 bits (2792), Expect = 0.0e+00
Identity = 502/596 (84.23%), Postives = 553/596 (92.79%), Query Frame = 1

Query: 1   MKVIDKITAATAGDGGRVVFSFEYFPPKTEDGVDNLFDRMDRMVAHNPSFCDITWGAGGS 60
           MK+I+KI AA+A D  RVVFSFE+FPPKTEDGVDNLF+RMDRMVAHNPSFCDITWGAGG+
Sbjct: 1   MKIIEKIRAASA-DPNRVVFSFEFFPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGGT 60

Query: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGLQNVLALRGDPPHGQ 120
           TADLTL+IANKMQN++CVETMMHLTCTNMPVEKIDHAL TIKSNGLQNVLALRGDPPHGQ
Sbjct: 61  TADLTLEIANKMQNIVCVETMMHLTCTNMPVEKIDHALHTIKSNGLQNVLALRGDPPHGQ 120

Query: 121 DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATTEGYQSDLAY 180
           DKFVQ+EGGFACA DLV+HIR KYGDYFGIT+AGYPEAHPDVI S+G+AT EGY++DLAY
Sbjct: 121 DKFVQVEGGFACARDLVQHIRAKYGDYFGITIAGYPEAHPDVIESDGLATPEGYRNDLAY 180

Query: 181 LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTSFCK 240
           LK K DAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGF+RMT FCK
Sbjct: 181 LKSKVDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFIRMTGFCK 240

Query: 241 TKIPDEVMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHMYTLNMEKSALAILM 300
           TKIP ++MAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLH+YTLNMEKSALAILM
Sbjct: 241 TKIPADIMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHLYTLNMEKSALAILM 300

Query: 301 NLGLIEESKVSRSLPWRRPTNVFRIREDVRPIFWANRPRSYISRTIGWEQYPHGRWSDSS 360
           NLGLIEESKVSRSLPWRRP NVFR++EDVRPIFWANRP+SYISRTIGW+QYPHGRW DS 
Sbjct: 301 NLGLIEESKVSRSLPWRRPANVFRVKEDVRPIFWANRPKSYISRTIGWDQYPHGRWGDSC 360

Query: 361 NPSYGALTDYQFMRPRARDKKLLEEWALSLKSLEDVYEKFMKYCLGKLRSSPWSELDGLQ 420
           NPSYGAL+DYQFMRPRARDKKL+EEWA+ LKS+ED+YE+F  YCLGKLRS+PWSELDGLQ
Sbjct: 361 NPSYGALSDYQFMRPRARDKKLVEEWAVPLKSVEDIYERFRLYCLGKLRSNPWSELDGLQ 420

Query: 421 AETRIISEQLGKINMKGFLSINSQPAVNGERSDSPSVGWGGSGGYVYQKAYIEFFCSREN 480
            ET+II+EQL KIN KGFL+INSQPAVNGE+SDSP+VGWGG GGYVYQKAY+EFFCS+E 
Sbjct: 421 PETKIINEQLEKINTKGFLTINSQPAVNGEKSDSPTVGWGGPGGYVYQKAYVEFFCSKEK 480

Query: 481 LDAVVDKCRGLPSLTYMAVNKEGIWVSNITQNDVNAVTWGVFPAKEIVQPTVVDPASFLV 540
           LDA+VDKC+   SLTYMAVNK+G W SN+ Q DVNAVTWGVFPAKEI+QPT+VDP SF V
Sbjct: 481 LDALVDKCKDRTSLTYMAVNKDGSWKSNVGQTDVNAVTWGVFPAKEIIQPTIVDPVSFNV 540

Query: 541 WKDEAFEIWSRGWASLYPEGDPSRKFLEEIQSSYYLVSLVDNDYVNSNLFAALEGF 597
           WKDEAFEIWSRGWASLYPE + SRK +EE+  S++LVSLVDNDY+N +LFA    F
Sbjct: 541 WKDEAFEIWSRGWASLYPEDEASRKLVEEVGGSHFLVSLVDNDYINGDLFAVFADF 595

BLAST of Cp4.1LG13g00150 vs. TrEMBL
Match: A0A0B2SRC1_GLYSO (Methylenetetrahydrofolate reductase OS=Glycine soja GN=glysoja_018451 PE=3 SV=1)

HSP 1 Score: 1080.1 bits (2792), Expect = 0.0e+00
Identity = 502/596 (84.23%), Postives = 553/596 (92.79%), Query Frame = 1

Query: 1   MKVIDKITAATAGDGGRVVFSFEYFPPKTEDGVDNLFDRMDRMVAHNPSFCDITWGAGGS 60
           MK+I+KI AA+A D  RVVFSFE+FPPKTEDGVDNLF+RMDRMVAHNPSFCDITWGAGG+
Sbjct: 1   MKIIEKIRAASA-DPNRVVFSFEFFPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGGT 60

Query: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGLQNVLALRGDPPHGQ 120
           TADLTL+IANKMQN++CVETMMHLTCTNMPVEKIDHAL TIKSNGLQNVLALRGDPPHGQ
Sbjct: 61  TADLTLEIANKMQNIVCVETMMHLTCTNMPVEKIDHALHTIKSNGLQNVLALRGDPPHGQ 120

Query: 121 DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATTEGYQSDLAY 180
           DKFVQ+EGGFACA DLV+HIR KYGDYFGIT+AGYPEAHPDVI S+G+AT EGY++DLAY
Sbjct: 121 DKFVQVEGGFACARDLVQHIRAKYGDYFGITIAGYPEAHPDVIESDGLATPEGYRNDLAY 180

Query: 181 LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTSFCK 240
           LK K DAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGF+RMT FCK
Sbjct: 181 LKSKVDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFIRMTGFCK 240

Query: 241 TKIPDEVMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHMYTLNMEKSALAILM 300
           TKIP ++MAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLH+YTLNMEKSALAILM
Sbjct: 241 TKIPADIMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHLYTLNMEKSALAILM 300

Query: 301 NLGLIEESKVSRSLPWRRPTNVFRIREDVRPIFWANRPRSYISRTIGWEQYPHGRWSDSS 360
           NLGLIEESKVSRSLPWRRP NVFR++EDVRPIFWANRP+SYISRTIGW+QYPHGRW DS 
Sbjct: 301 NLGLIEESKVSRSLPWRRPANVFRVKEDVRPIFWANRPKSYISRTIGWDQYPHGRWGDSC 360

Query: 361 NPSYGALTDYQFMRPRARDKKLLEEWALSLKSLEDVYEKFMKYCLGKLRSSPWSELDGLQ 420
           NPSYGAL+DYQFMRPRARDKKL+EEWA+ LKS+ED+YE+F  YCLGKLRS+PWSELDGLQ
Sbjct: 361 NPSYGALSDYQFMRPRARDKKLVEEWAVPLKSVEDIYERFRLYCLGKLRSNPWSELDGLQ 420

Query: 421 AETRIISEQLGKINMKGFLSINSQPAVNGERSDSPSVGWGGSGGYVYQKAYIEFFCSREN 480
            ET+II+EQL KIN KGFL+INSQPAVNGE+SDSP+VGWGG GGYVYQKAY+EFFCS+E 
Sbjct: 421 PETKIINEQLEKINTKGFLTINSQPAVNGEKSDSPTVGWGGPGGYVYQKAYVEFFCSKEK 480

Query: 481 LDAVVDKCRGLPSLTYMAVNKEGIWVSNITQNDVNAVTWGVFPAKEIVQPTVVDPASFLV 540
           LDA+VDKC+   SLTYMAVNK+G W SN+ Q DVNAVTWGVFPAKEI+QPT+VDP SF V
Sbjct: 481 LDALVDKCKDRTSLTYMAVNKDGSWKSNVGQTDVNAVTWGVFPAKEIIQPTIVDPVSFNV 540

Query: 541 WKDEAFEIWSRGWASLYPEGDPSRKFLEEIQSSYYLVSLVDNDYVNSNLFAALEGF 597
           WKDEAFEIWSRGWASLYPE + SRK +EE+  S++LVSLVDNDY+N +LFA    F
Sbjct: 541 WKDEAFEIWSRGWASLYPEDEASRKLVEEVGGSHFLVSLVDNDYINGDLFAVFADF 595

BLAST of Cp4.1LG13g00150 vs. TAIR10
Match: AT2G44160.1 (AT2G44160.1 methylenetetrahydrofolate reductase 2)

HSP 1 Score: 1030.0 bits (2662), Expect = 5.7e-301
Identity = 477/591 (80.71%), Postives = 535/591 (90.52%), Query Frame = 1

Query: 1   MKVIDKITAATAGDGGRVVFSFEYFPPKTEDGVDNLFDRMDRMVAHNPSFCDITWGAGGS 60
           MKVIDKI +    D G+  FSFE+FPPKTEDGVDNLF+RMDRMVA+ P+FCDITWGAGGS
Sbjct: 1   MKVIDKIQSLA--DEGKTAFSFEFFPPKTEDGVDNLFERMDRMVAYGPTFCDITWGAGGS 60

Query: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGLQNVLALRGDPPHGQ 120
           TADLTLDIA++MQN++CVE+MMHLTCTNMPVEKIDHAL+TI+SNG+QNVLALRGDPPHGQ
Sbjct: 61  TADLTLDIASRMQNVVCVESMMHLTCTNMPVEKIDHALETIRSNGIQNVLALRGDPPHGQ 120

Query: 121 DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATTEGYQSDLAY 180
           DKFVQ+EGGF CALDLV HIR KYGDYFGITVAGYPEAHPDVIG NG+A+ E YQSDL Y
Sbjct: 121 DKFVQVEGGFDCALDLVNHIRSKYGDYFGITVAGYPEAHPDVIGENGLASNEAYQSDLEY 180

Query: 181 LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTSFCK 240
           LKKK DAGADLIVTQLFYDTDIFLKFVNDCRQIGI+CPIVPGIMPINNY+GFLRMT FCK
Sbjct: 181 LKKKIDAGADLIVTQLFYDTDIFLKFVNDCRQIGISCPIVPGIMPINNYRGFLRMTGFCK 240

Query: 241 TKIPDEVMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHMYTLNMEKSALAILM 300
           TKIP EVMAALEPIKDNEEAVKAYGIHLGTEMCKK+LAHG+K+LH+YTLNMEKSALAILM
Sbjct: 241 TKIPVEVMAALEPIKDNEEAVKAYGIHLGTEMCKKMLAHGVKSLHLYTLNMEKSALAILM 300

Query: 301 NLGLIEESKVSRSLPWRRPTNVFRIREDVRPIFWANRPRSYISRTIGWEQYPHGRWSDSS 360
           NLG+I+ESK+SRSLPWRRP NVFR +EDVRPIFWANRP+SYISRT GWE +P GRW DS 
Sbjct: 301 NLGMIDESKISRSLPWRRPANVFRTKEDVRPIFWANRPKSYISRTKGWEDFPQGRWGDSR 360

Query: 361 NPSYGALTDYQFMRPRARDKKLLEEWALSLKSLEDVYEKFMKYCLGKLRSSPWSELDGLQ 420
           + SYGAL+D+QF RPRARDKKL +EW + LKS+ED+ EKF + CLG L+SSPWSELDGLQ
Sbjct: 361 SASYGALSDHQFSRPRARDKKLQQEWVVPLKSVEDIQEKFKELCLGNLKSSPWSELDGLQ 420

Query: 421 AETRIISEQLGKINMKGFLSINSQPAVNGERSDSPSVGWGGSGGYVYQKAYIEFFCSREN 480
            ETRII+EQL K+N KGFL+INSQP+VN ERSDSP+VGWGG  GYVYQKAY+EFFCS+E 
Sbjct: 421 PETRIINEQLIKVNSKGFLTINSQPSVNAERSDSPTVGWGGPVGYVYQKAYLEFFCSKEK 480

Query: 481 LDAVVDKCRGLPSLTYMAVNKEGIWVSNITQNDVNAVTWGVFPAKEIVQPTVVDPASFLV 540
           LDAVV+KC+ LPS+TYMAVNK   WVSN  Q DVNAVTWGVFPAKEI+QPT+VDPASF V
Sbjct: 481 LDAVVEKCKALPSITYMAVNKGEQWVSNTAQADVNAVTWGVFPAKEIIQPTIVDPASFNV 540

Query: 541 WKDEAFEIWSRGWASLYPEGDPSRKFLEEIQSSYYLVSLVDNDYVNSNLFA 592
           WKDEAFE WSR WA+LYPE DPSR  LEE+++SYYLVSLV+NDY+N ++FA
Sbjct: 541 WKDEAFETWSRSWANLYPEADPSRNLLEEVKNSYYLVSLVENDYINGDIFA 589

BLAST of Cp4.1LG13g00150 vs. TAIR10
Match: AT3G59970.3 (AT3G59970.3 methylenetetrahydrofolate reductase 1)

HSP 1 Score: 999.2 bits (2582), Expect = 1.1e-291
Identity = 454/591 (76.82%), Postives = 528/591 (89.34%), Query Frame = 1

Query: 1   MKVIDKITAATAGDGGRVVFSFEYFPPKTEDGVDNLFDRMDRMVAHNPSFCDITWGAGGS 60
           MKV+DKI + T  + G+  FSFE+FPPKTEDGV+NLF+RMDR+V++ P+FCDITWGAGGS
Sbjct: 1   MKVVDKIKSVT--EQGQTAFSFEFFPPKTEDGVENLFERMDRLVSYGPTFCDITWGAGGS 60

Query: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGLQNVLALRGDPPHGQ 120
           TADLTL+IA++MQN+ICVETMMHLTCTNMP+EKIDHAL+TI+SNG+QNVLALRGDPPHGQ
Sbjct: 61  TADLTLEIASRMQNVICVETMMHLTCTNMPIEKIDHALETIRSNGIQNVLALRGDPPHGQ 120

Query: 121 DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATTEGYQSDLAY 180
           DKFVQ+EGGFACALDLV HIR KYGDYFGITVAGYPEAHPDVI ++G+AT E YQSDLAY
Sbjct: 121 DKFVQVEGGFACALDLVNHIRSKYGDYFGITVAGYPEAHPDVIEADGLATPESYQSDLAY 180

Query: 181 LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTSFCK 240
           LKKK DAGADLIVTQLFYDTDIFLKFVNDCRQIGI CPIVPGIMPI+NYKGFLRM  FCK
Sbjct: 181 LKKKVDAGADLIVTQLFYDTDIFLKFVNDCRQIGINCPIVPGIMPISNYKGFLRMAGFCK 240

Query: 241 TKIPDEVMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHMYTLNMEKSALAILM 300
           TKIP E+ AALEPIKDN+EAVKAYGIH  TEMCKKILAHGI +LH+YTLN++KSA+ ILM
Sbjct: 241 TKIPAELTAALEPIKDNDEAVKAYGIHFATEMCKKILAHGITSLHLYTLNVDKSAIGILM 300

Query: 301 NLGLIEESKVSRSLPWRRPTNVFRIREDVRPIFWANRPRSYISRTIGWEQYPHGRWSDSS 360
           NLGLI+ESK+SRSLPWRRP NVFR +EDVRPIFWANRP+SYISRT GW  +PHGRW DS 
Sbjct: 301 NLGLIDESKISRSLPWRRPANVFRTKEDVRPIFWANRPKSYISRTKGWNDFPHGRWGDSH 360

Query: 361 NPSYGALTDYQFMRPRARDKKLLEEWALSLKSLEDVYEKFMKYCLGKLRSSPWSELDGLQ 420
           + +Y  L+DYQF RP+ RDKKL +EW + LKS+EDV EKF + C+G L+SSPWSELDGLQ
Sbjct: 361 SAAYSTLSDYQFARPKGRDKKLQQEWVVPLKSIEDVQEKFKELCIGNLKSSPWSELDGLQ 420

Query: 421 AETRIISEQLGKINMKGFLSINSQPAVNGERSDSPSVGWGGSGGYVYQKAYIEFFCSREN 480
            ET+II+EQLGKIN  GFL+INSQP+VN  +SDSP++GWGG GGYVYQKAY+EFFCS++ 
Sbjct: 421 PETKIINEQLGKINSNGFLTINSQPSVNAAKSDSPAIGWGGPGGYVYQKAYLEFFCSKDK 480

Query: 481 LDAVVDKCRGLPSLTYMAVNKEGIWVSNITQNDVNAVTWGVFPAKEIVQPTVVDPASFLV 540
           LD +V+K +  PS+TYMAVNK   WVSN  ++DVNAVTWGVFPAKE++QPT+VDPASF V
Sbjct: 481 LDTLVEKSKAFPSITYMAVNKSENWVSNTGESDVNAVTWGVFPAKEVIQPTIVDPASFKV 540

Query: 541 WKDEAFEIWSRGWASLYPEGDPSRKFLEEIQSSYYLVSLVDNDYVNSNLFA 592
           WKDEAFEIWSR WA+LYPE DPSRK LEE+++SYYLVSLVDN+Y+N ++F+
Sbjct: 541 WKDEAFEIWSRSWANLYPEDDPSRKLLEEVKNSYYLVSLVDNNYINGDIFS 589

BLAST of Cp4.1LG13g00150 vs. NCBI nr
Match: gi|778668497|ref|XP_004138970.2| (PREDICTED: methylenetetrahydrofolate reductase 2 [Cucumis sativus])

HSP 1 Score: 1190.6 bits (3079), Expect = 0.0e+00
Identity = 566/596 (94.97%), Postives = 581/596 (97.48%), Query Frame = 1

Query: 1   MKVIDKITAATAGDGGRVVFSFEYFPPKTEDGVDNLFDRMDRMVAHNPSFCDITWGAGGS 60
           MKVIDKI AATAGDGGRVVFSFEYFPPKTEDGVDNLF+RMDRMVAHNPSFCDITWGAGGS
Sbjct: 43  MKVIDKIRAATAGDGGRVVFSFEYFPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGGS 102

Query: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGLQNVLALRGDPPHGQ 120
           TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGLQNVLALRGDPPHGQ
Sbjct: 103 TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGLQNVLALRGDPPHGQ 162

Query: 121 DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATTEGYQSDLAY 180
           DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATTEGYQSDLAY
Sbjct: 163 DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATTEGYQSDLAY 222

Query: 181 LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTSFCK 240
           LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMT FCK
Sbjct: 223 LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCK 282

Query: 241 TKIPDEVMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHMYTLNMEKSALAILM 300
           TKIPDE+MAALEPIKDNEEAVKAYGIHLGTEMCKKIL HGIKTLH+YTLNMEKSALAILM
Sbjct: 283 TKIPDEIMAALEPIKDNEEAVKAYGIHLGTEMCKKILTHGIKTLHIYTLNMEKSALAILM 342

Query: 301 NLGLIEESKVSRSLPWRRPTNVFRIREDVRPIFWANRPRSYISRTIGWEQYPHGRWSDSS 360
           NLGLIEESKVSRSLPWRRPTNVFR+REDVRPIFWANRPRSYISRTIGW+QYPHGRWSDS 
Sbjct: 343 NLGLIEESKVSRSLPWRRPTNVFRVREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDSR 402

Query: 361 NPSYGALTDYQFMRPRARDKKLLEEWALSLKSLEDVYEKFMKYCLGKLRSSPWSELDGLQ 420
           NPSYGALTDYQFMRPRARDKKLLEEWAL LKS+EDVYEKFMKYCLGKLRSSPWSEL+GLQ
Sbjct: 403 NPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELEGLQ 462

Query: 421 AETRIISEQLGKINMKGFLSINSQPAVNGERSDSPSVGWGGSGGYVYQKAYIEFFCSREN 480
           AETR+ISEQLGKINMKGFLSINSQPAVNGERSDSP VGWGG GGYVYQKAYIEFFCSRE 
Sbjct: 463 AETRMISEQLGKINMKGFLSINSQPAVNGERSDSPCVGWGGPGGYVYQKAYIEFFCSREK 522

Query: 481 LDAVVDKCRGLPSLTYMAVNKEGIWVSNITQNDVNAVTWGVFPAKEIVQPTVVDPASFLV 540
           LDA+VDKCR LPSLTYMAVNKEGIWVSN TQ DVNAVTWGVFPAKEI+QPTVVDPASF+V
Sbjct: 523 LDAIVDKCRALPSLTYMAVNKEGIWVSNATQKDVNAVTWGVFPAKEIIQPTVVDPASFIV 582

Query: 541 WKDEAFEIWSRGWASLYPEGDPSRKFLEEIQSSYYLVSLVDNDYVNSNLFAALEGF 597
           WKDEAFEIWSRGWA+LYPEGD SRK L+EIQSSYYLVSLVDNDY+NS+LFA LE F
Sbjct: 583 WKDEAFEIWSRGWAALYPEGDWSRKLLDEIQSSYYLVSLVDNDYINSDLFAILEDF 638

BLAST of Cp4.1LG13g00150 vs. NCBI nr
Match: gi|1021576668|ref|XP_016179517.1| (PREDICTED: probable methylenetetrahydrofolate reductase [Arachis ipaensis])

HSP 1 Score: 1096.3 bits (2834), Expect = 0.0e+00
Identity = 509/596 (85.40%), Postives = 555/596 (93.12%), Query Frame = 1

Query: 1   MKVIDKITAATAGDGGRVVFSFEYFPPKTEDGVDNLFDRMDRMVAHNPSFCDITWGAGGS 60
           MKVI+KI AA A D  RVVFSFE+FPPKTEDGVDNLF+RMDRMVAHNPSFCDITWGAGGS
Sbjct: 1   MKVIEKIRAAAAADPDRVVFSFEFFPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGGS 60

Query: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGLQNVLALRGDPPHGQ 120
           TADLTL+IANKMQNM+CVETMMHLTCTNMPVEKIDHALQTIKSNGLQNVLALRGDPPHGQ
Sbjct: 61  TADLTLEIANKMQNMVCVETMMHLTCTNMPVEKIDHALQTIKSNGLQNVLALRGDPPHGQ 120

Query: 121 DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATTEGYQSDLAY 180
           DKFVQIEGGFACA DLVKHIR KYGDYFGITVAGYPEAHPDVIGS+G+AT EGYQ+DLAY
Sbjct: 121 DKFVQIEGGFACARDLVKHIRAKYGDYFGITVAGYPEAHPDVIGSDGLATPEGYQNDLAY 180

Query: 181 LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTSFCK 240
           LK K DAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMT FCK
Sbjct: 181 LKSKVDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCK 240

Query: 241 TKIPDEVMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHMYTLNMEKSALAILM 300
           TKIP ++ AALEPIKDNEEAVK+YGIHLGTEMCKKILAHGIKTLHMYTLNMEKSALAILM
Sbjct: 241 TKIPADIFAALEPIKDNEEAVKSYGIHLGTEMCKKILAHGIKTLHMYTLNMEKSALAILM 300

Query: 301 NLGLIEESKVSRSLPWRRPTNVFRIREDVRPIFWANRPRSYISRTIGWEQYPHGRWSDSS 360
           NLGLIEE+K++RSLPWRRP NVFR+REDVRPIFWANRP+SYISRTIGW+QYPHGRW DS 
Sbjct: 301 NLGLIEETKINRSLPWRRPANVFRVREDVRPIFWANRPKSYISRTIGWDQYPHGRWGDSR 360

Query: 361 NPSYGALTDYQFMRPRARDKKLLEEWALSLKSLEDVYEKFMKYCLGKLRSSPWSELDGLQ 420
           NPSYGALTDYQFMRPRARDKKL+EEW + L+S+ED+YE+F +YCLGKLRS+PWSELDGLQ
Sbjct: 361 NPSYGALTDYQFMRPRARDKKLVEEWVVPLRSIEDIYERFKQYCLGKLRSNPWSELDGLQ 420

Query: 421 AETRIISEQLGKINMKGFLSINSQPAVNGERSDSPSVGWGGSGGYVYQKAYIEFFCSREN 480
            ET+II+EQL KIN+KGFL+INSQPAVNGE+SDS +VGWGG GGYVYQKAY+EFFCS+E 
Sbjct: 421 PETKIINEQLEKINLKGFLTINSQPAVNGEKSDSATVGWGGPGGYVYQKAYVEFFCSKEK 480

Query: 481 LDAVVDKCRGLPSLTYMAVNKEGIWVSNITQNDVNAVTWGVFPAKEIVQPTVVDPASFLV 540
           LDA+V+KC+   SLT+MAVNKEG W SN+ Q DVNAVTWGVFPAKEI+QPT+VDP SF+V
Sbjct: 481 LDALVEKCKNRSSLTFMAVNKEGSWRSNVGQTDVNAVTWGVFPAKEIIQPTIVDPVSFMV 540

Query: 541 WKDEAFEIWSRGWASLYPEGDPSRKFLEEIQSSYYLVSLVDNDYVNSNLFAALEGF 597
           WKDEAFEIWSRGWA LYPEGD SRK +EE+ +SY+LVSLVDNDYVN +LFA    F
Sbjct: 541 WKDEAFEIWSRGWACLYPEGDASRKLVEEVGNSYFLVSLVDNDYVNGDLFAVFADF 596

BLAST of Cp4.1LG13g00150 vs. NCBI nr
Match: gi|1009142164|ref|XP_015888576.1| (PREDICTED: methylenetetrahydrofolate reductase 2-like [Ziziphus jujuba])

HSP 1 Score: 1088.6 bits (2814), Expect = 0.0e+00
Identity = 508/596 (85.23%), Postives = 554/596 (92.95%), Query Frame = 1

Query: 1   MKVIDKITAATAGDGGRVVFSFEYFPPKTEDGVDNLFDRMDRMVAHNPSFCDITWGAGGS 60
           MKVI+KI  A+  D  +VVFSFE+FPPKTEDGVDNLF+RMDRMVAHNP+FCDITWGAGGS
Sbjct: 1   MKVIEKIQDASL-DEKKVVFSFEFFPPKTEDGVDNLFERMDRMVAHNPTFCDITWGAGGS 60

Query: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGLQNVLALRGDPPHGQ 120
           TADLTLDIAN+MQN+ICVE+MMHLTCTNMPVEKIDHAL TIKSNGLQNVLALRGDPPHGQ
Sbjct: 61  TADLTLDIANRMQNIICVESMMHLTCTNMPVEKIDHALDTIKSNGLQNVLALRGDPPHGQ 120

Query: 121 DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATTEGYQSDLAY 180
           DKFVQ EGGFACALDLVKHIR KYGDYFGITVAGYPEAHPDVIGSNGVA+ EGYQSDLAY
Sbjct: 121 DKFVQTEGGFACALDLVKHIRGKYGDYFGITVAGYPEAHPDVIGSNGVASLEGYQSDLAY 180

Query: 181 LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTSFCK 240
           LK+K DAGADLI+TQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMT FCK
Sbjct: 181 LKRKVDAGADLIITQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCK 240

Query: 241 TKIPDEVMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHMYTLNMEKSALAILM 300
           TKIP E+ AALEPIKDNEEAVKAYGIHLGTEMCKKILAHGI+TLH+YTLNMEKSALAILM
Sbjct: 241 TKIPAEITAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIRTLHLYTLNMEKSALAILM 300

Query: 301 NLGLIEESKVSRSLPWRRPTNVFRIREDVRPIFWANRPRSYISRTIGWEQYPHGRWSDSS 360
           NLGLIEESK+ R LPWRRPTNVFR++EDVRPIFWANRP+SY+SRTIGW+QYPHGRW DS 
Sbjct: 301 NLGLIEESKIQRPLPWRRPTNVFRVKEDVRPIFWANRPKSYLSRTIGWDQYPHGRWGDSR 360

Query: 361 NPSYGALTDYQFMRPRARDKKLLEEWALSLKSLEDVYEKFMKYCLGKLRSSPWSELDGLQ 420
           NPSYGAL+DYQFMRPRAR KKL+EEW   L +++DVYEKF K+CLGKLRSSPWSELDGLQ
Sbjct: 361 NPSYGALSDYQFMRPRARGKKLIEEWVTPLTNIDDVYEKFKKFCLGKLRSSPWSELDGLQ 420

Query: 421 AETRIISEQLGKINMKGFLSINSQPAVNGERSDSPSVGWGGSGGYVYQKAYIEFFCSREN 480
            ETRII++QLG+IN KGFL+INSQPAVNGERSDSP VGWGG GGYVYQKAY+EFFCSRE 
Sbjct: 421 PETRIINDQLGRINTKGFLTINSQPAVNGERSDSPVVGWGGPGGYVYQKAYLEFFCSREK 480

Query: 481 LDAVVDKCRGLPSLTYMAVNKEGIWVSNITQNDVNAVTWGVFPAKEIVQPTVVDPASFLV 540
           LDA+VDKCR LPSLTY+AVNKEG W+SNI Q+ VNAVTWGVFPA+EI+QPTVVDPASF+V
Sbjct: 481 LDALVDKCRALPSLTYVAVNKEGSWLSNIGQSQVNAVTWGVFPAREIIQPTVVDPASFMV 540

Query: 541 WKDEAFEIWSRGWASLYPEGDPSRKFLEEIQSSYYLVSLVDNDYVNSNLFAALEGF 597
           WKDEAFEIWSRGWA  YPEGD SRK L+EI+++Y+LVSLVDNDY+N +LFA    F
Sbjct: 541 WKDEAFEIWSRGWARHYPEGDASRKLLQEIENAYFLVSLVDNDYINGDLFAVFADF 595

BLAST of Cp4.1LG13g00150 vs. NCBI nr
Match: gi|356509485|ref|XP_003523478.1| (PREDICTED: methylenetetrahydrofolate reductase 2 [Glycine max])

HSP 1 Score: 1080.1 bits (2792), Expect = 0.0e+00
Identity = 502/596 (84.23%), Postives = 553/596 (92.79%), Query Frame = 1

Query: 1   MKVIDKITAATAGDGGRVVFSFEYFPPKTEDGVDNLFDRMDRMVAHNPSFCDITWGAGGS 60
           MK+I+KI AA+A D  RVVFSFE+FPPKTEDGVDNLF+RMDRMVAHNPSFCDITWGAGG+
Sbjct: 1   MKIIEKIRAASA-DPNRVVFSFEFFPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGGT 60

Query: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGLQNVLALRGDPPHGQ 120
           TADLTL+IANKMQN++CVETMMHLTCTNMPVEKIDHAL TIKSNGLQNVLALRGDPPHGQ
Sbjct: 61  TADLTLEIANKMQNIVCVETMMHLTCTNMPVEKIDHALHTIKSNGLQNVLALRGDPPHGQ 120

Query: 121 DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATTEGYQSDLAY 180
           DKFVQ+EGGFACA DLV+HIR KYGDYFGIT+AGYPEAHPDVI S+G+AT EGY++DLAY
Sbjct: 121 DKFVQVEGGFACARDLVQHIRAKYGDYFGITIAGYPEAHPDVIESDGLATPEGYRNDLAY 180

Query: 181 LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTSFCK 240
           LK K DAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGF+RMT FCK
Sbjct: 181 LKSKVDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFIRMTGFCK 240

Query: 241 TKIPDEVMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHMYTLNMEKSALAILM 300
           TKIP ++MAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLH+YTLNMEKSALAILM
Sbjct: 241 TKIPADIMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHLYTLNMEKSALAILM 300

Query: 301 NLGLIEESKVSRSLPWRRPTNVFRIREDVRPIFWANRPRSYISRTIGWEQYPHGRWSDSS 360
           NLGLIEESKVSRSLPWRRP NVFR++EDVRPIFWANRP+SYISRTIGW+QYPHGRW DS 
Sbjct: 301 NLGLIEESKVSRSLPWRRPANVFRVKEDVRPIFWANRPKSYISRTIGWDQYPHGRWGDSC 360

Query: 361 NPSYGALTDYQFMRPRARDKKLLEEWALSLKSLEDVYEKFMKYCLGKLRSSPWSELDGLQ 420
           NPSYGAL+DYQFMRPRARDKKL+EEWA+ LKS+ED+YE+F  YCLGKLRS+PWSELDGLQ
Sbjct: 361 NPSYGALSDYQFMRPRARDKKLVEEWAVPLKSVEDIYERFRLYCLGKLRSNPWSELDGLQ 420

Query: 421 AETRIISEQLGKINMKGFLSINSQPAVNGERSDSPSVGWGGSGGYVYQKAYIEFFCSREN 480
            ET+II+EQL KIN KGFL+INSQPAVNGE+SDSP+VGWGG GGYVYQKAY+EFFCS+E 
Sbjct: 421 PETKIINEQLEKINTKGFLTINSQPAVNGEKSDSPTVGWGGPGGYVYQKAYVEFFCSKEK 480

Query: 481 LDAVVDKCRGLPSLTYMAVNKEGIWVSNITQNDVNAVTWGVFPAKEIVQPTVVDPASFLV 540
           LDA+VDKC+   SLTYMAVNK+G W SN+ Q DVNAVTWGVFPAKEI+QPT+VDP SF V
Sbjct: 481 LDALVDKCKDRTSLTYMAVNKDGSWKSNVGQTDVNAVTWGVFPAKEIIQPTIVDPVSFNV 540

Query: 541 WKDEAFEIWSRGWASLYPEGDPSRKFLEEIQSSYYLVSLVDNDYVNSNLFAALEGF 597
           WKDEAFEIWSRGWASLYPE + SRK +EE+  S++LVSLVDNDY+N +LFA    F
Sbjct: 541 WKDEAFEIWSRGWASLYPEDEASRKLVEEVGGSHFLVSLVDNDYINGDLFAVFADF 595

BLAST of Cp4.1LG13g00150 vs. NCBI nr
Match: gi|356517828|ref|XP_003527588.1| (PREDICTED: methylenetetrahydrofolate reductase 2 [Glycine max])

HSP 1 Score: 1080.1 bits (2792), Expect = 0.0e+00
Identity = 502/596 (84.23%), Postives = 551/596 (92.45%), Query Frame = 1

Query: 1   MKVIDKITAATAGDGGRVVFSFEYFPPKTEDGVDNLFDRMDRMVAHNPSFCDITWGAGGS 60
           MK+I+KI AA+A D  RVVFSFE+FPPKTEDGVDNLF+RMDRMV HNPSFCDITWGAGG+
Sbjct: 1   MKIIEKIHAASA-DPNRVVFSFEFFPPKTEDGVDNLFERMDRMVVHNPSFCDITWGAGGT 60

Query: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGLQNVLALRGDPPHGQ 120
           TADLTL+IANKMQN++CVETMMHLTCTNMPVEKIDHAL TIKSNGLQNVLALRGDPPHGQ
Sbjct: 61  TADLTLEIANKMQNIVCVETMMHLTCTNMPVEKIDHALHTIKSNGLQNVLALRGDPPHGQ 120

Query: 121 DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATTEGYQSDLAY 180
           DKFVQ+EGGFACA DLV+HIR KYGDYFGITVAGYPEAHPDVI S+G+AT+EGYQ+DLAY
Sbjct: 121 DKFVQVEGGFACARDLVQHIRAKYGDYFGITVAGYPEAHPDVIESDGLATSEGYQNDLAY 180

Query: 181 LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTSFCK 240
           LK K DAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGF+RMT FCK
Sbjct: 181 LKSKVDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFIRMTGFCK 240

Query: 241 TKIPDEVMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHMYTLNMEKSALAILM 300
           TKIP ++MAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGI TLH+YTLNMEKSALAILM
Sbjct: 241 TKIPADIMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGINTLHLYTLNMEKSALAILM 300

Query: 301 NLGLIEESKVSRSLPWRRPTNVFRIREDVRPIFWANRPRSYISRTIGWEQYPHGRWSDSS 360
           NLGLIEE+KVSRSLPWRRP NVFR++EDVRPIFWANRP+SYISRTIGW+QYPHGRW DS 
Sbjct: 301 NLGLIEETKVSRSLPWRRPANVFRVKEDVRPIFWANRPKSYISRTIGWDQYPHGRWGDSC 360

Query: 361 NPSYGALTDYQFMRPRARDKKLLEEWALSLKSLEDVYEKFMKYCLGKLRSSPWSELDGLQ 420
           NPSYGAL+DYQFMRPRARDKKL+EEWA+ LKS+ED+YE+F  YCLGKLRS+PWSELDGLQ
Sbjct: 361 NPSYGALSDYQFMRPRARDKKLVEEWAVPLKSIEDIYERFRLYCLGKLRSNPWSELDGLQ 420

Query: 421 AETRIISEQLGKINMKGFLSINSQPAVNGERSDSPSVGWGGSGGYVYQKAYIEFFCSREN 480
            ET+II+E L KIN KGFL+INSQPAVNGE+SDSP+VGWGG GGYVYQKAY+EFFCS+E 
Sbjct: 421 PETKIINELLEKINTKGFLTINSQPAVNGEKSDSPTVGWGGPGGYVYQKAYVEFFCSKEK 480

Query: 481 LDAVVDKCRGLPSLTYMAVNKEGIWVSNITQNDVNAVTWGVFPAKEIVQPTVVDPASFLV 540
           LDA+VDKC+   SLTYMAVNK+G W SN+ Q DVNAVTWGVFPAKEI+QPT+VDP SF V
Sbjct: 481 LDALVDKCKDRTSLTYMAVNKDGSWKSNVGQTDVNAVTWGVFPAKEIIQPTIVDPVSFNV 540

Query: 541 WKDEAFEIWSRGWASLYPEGDPSRKFLEEIQSSYYLVSLVDNDYVNSNLFAALEGF 597
           WKDEAFEIWSRGWASLYPEGD SRK +EE+  SY+LVSLVDNDY+N +LF     F
Sbjct: 541 WKDEAFEIWSRGWASLYPEGDASRKLVEEVGGSYFLVSLVDNDYINGDLFTVFADF 595

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
MTHR2_ARATH1.0e-29980.71Methylenetetrahydrofolate reductase 2 OS=Arabidopsis thaliana GN=MTHFR2 PE=1 SV=... [more]
MTHR_ORYSJ2.5e-29880.98Probable methylenetetrahydrofolate reductase OS=Oryza sativa subsp. japonica GN=... [more]
MTHR1_MAIZE5.8e-29579.46Methylenetetrahydrofolate reductase 1 OS=Zea mays PE=2 SV=1[more]
MTHR1_ARATH1.9e-29076.82Methylenetetrahydrofolate reductase 1 OS=Arabidopsis thaliana GN=MTHFR1 PE=1 SV=... [more]
MTHR_HUMAN4.3e-14944.58Methylenetetrahydrofolate reductase OS=Homo sapiens GN=MTHFR PE=1 SV=3[more]
Match NameE-valueIdentityDescription
A0A0A0LIC6_CUCSA0.0e+0094.97Methylenetetrahydrofolate reductase OS=Cucumis sativus GN=Csa_2G149470 PE=3 SV=1[more]
I1KG02_SOYBN0.0e+0084.23Methylenetetrahydrofolate reductase OS=Glycine max GN=GLYMA_06G325300 PE=3 SV=1[more]
A0A0B2QJZ4_GLYSO0.0e+0084.23Methylenetetrahydrofolate reductase OS=Glycine soja GN=glysoja_033227 PE=3 SV=1[more]
I1JZE2_SOYBN0.0e+0084.23Methylenetetrahydrofolate reductase OS=Glycine max GN=GLYMA_04G256100 PE=3 SV=1[more]
A0A0B2SRC1_GLYSO0.0e+0084.23Methylenetetrahydrofolate reductase OS=Glycine soja GN=glysoja_018451 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G44160.15.7e-30180.71 methylenetetrahydrofolate reductase 2[more]
AT3G59970.31.1e-29176.82 methylenetetrahydrofolate reductase 1[more]
Match NameE-valueIdentityDescription
gi|778668497|ref|XP_004138970.2|0.0e+0094.97PREDICTED: methylenetetrahydrofolate reductase 2 [Cucumis sativus][more]
gi|1021576668|ref|XP_016179517.1|0.0e+0085.40PREDICTED: probable methylenetetrahydrofolate reductase [Arachis ipaensis][more]
gi|1009142164|ref|XP_015888576.1|0.0e+0085.23PREDICTED: methylenetetrahydrofolate reductase 2-like [Ziziphus jujuba][more]
gi|356509485|ref|XP_003523478.1|0.0e+0084.23PREDICTED: methylenetetrahydrofolate reductase 2 [Glycine max][more]
gi|356517828|ref|XP_003527588.1|0.0e+0084.23PREDICTED: methylenetetrahydrofolate reductase 2 [Glycine max][more]
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
GO:0006555methionine metabolic process
Vocabulary: Molecular Function
TermDefinition
GO:0004489methylenetetrahydrofolate reductase (NAD(P)H) activity
Vocabulary: INTERPRO
TermDefinition
IPR004621Fadh2_euk
IPR003171Mehydrof_redctse
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009086 methionine biosynthetic process
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0035999 tetrahydrofolate interconversion
biological_process GO:0006555 methionine metabolic process
biological_process GO:0046653 tetrahydrofolate metabolic process
cellular_component GO:0005829 cytosol
cellular_component GO:0005575 cellular_component
molecular_function GO:0004489 methylenetetrahydrofolate reductase (NAD(P)H) activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG13g00150.1Cp4.1LG13g00150.1mRNA


Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003171Methylenetetrahydrofolate reductasePFAMPF02219MTHFRcoord: 15..303
score: 2.2E
IPR004621Eukaryotic-type methylenetetrahydrofolate reductaseTIGRFAMsTIGR00677TIGR00677coord: 19..307
score: 1.6E
NoneNo IPR availablePANTHERPTHR21091METHYLTETRAHYDROFOLATE:HOMOCYSTEINE METHYLTRANSFERASE RELATEDcoord: 131..149
score: 6.9E-239coord: 180..593
score: 6.9E
NoneNo IPR availablePANTHERPTHR21091:SF31METHYLENETETRAHYDROFOLATE REDUCTASEcoord: 180..593
score: 6.9E-239coord: 131..149
score: 6.9E

The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Cp4.1LG13g00150CmaCh15G014320Cucurbita maxima (Rimu)cmacpeB306
Cp4.1LG13g00150CmoCh15G015100Cucurbita moschata (Rifu)cmocpeB269
Cp4.1LG13g00150MELO3C020408Melon (DHL92) v3.5.1cpemeB174
Cp4.1LG13g00150Lsi09G010670Bottle gourd (USVL1VR-Ls)cpelsiB137
Cp4.1LG13g00150Carg20746Silver-seed gourdcarcpeB1040
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Cp4.1LG13g00150Wax gourdcpewgoB0223
Cp4.1LG13g00150Bottle gourd (USVL1VR-Ls)cpelsiB132
Cp4.1LG13g00150Melon (DHL92) v3.6.1cpemedB209