Cp4.1LG12g05860 (gene) Cucurbita pepo (Zucchini)

Overview
NameCp4.1LG12g05860
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
DescriptionPectinesterase
LocationCp4.1LG12 : 7922749 .. 7925587 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AAATATTATAAATCTACTCTTCATCTTCCTTCTTGACAGCCACATAAATCTCCCCTTCATCTTCTTCCCTCCTCTCTCTGTTTCAGACATGTACGGCCTCAGAAACAGAGCTCAAAGCTCTTCAAATCCCACCGCCTCCATTTCCGCCATTTTCACCTTCTTTTCCGTTCTTATCCTTTGCTCCTTCAAACCCATTTCTTCAAATCCCCAAACCCACCAAACCGCTCGATCCCTTTGCAATGGCACTCTGTTTCCAAATCTCTGTTTCTCAAAGCTCTCCTCTTTTCCCCAACTCGCCTCCCTCTCGCCGGAAAAGCTCGCCGGCTCCGCCCTCAACTTCACCAACGACGAGGTTCACGGTGCCTACGCCAATTGCTCCAAGCTCAAGACTCTCTTCTACGGAGGTCTGAGTCCCACTGACCGCCGCGCCCTCGATGACTGCCTTGAGCTTCTCGATGGCAGCATTGCCGAACTCCAAACTTCGATTTACGATCTATCACCGTCGCAGTCCGCCGCCCGCCACTCCCACGATCTGTTGACGTTGGTCAGCGCCGCCATGACTAACCACCGGACTTGTCTTGATGGGTTCTATAATAGTTCCGGGACTGTGAGGAAACGGGTGGAGCTGTATTTGGGGAAAATCGAACAGCATTTGAGTATTGATTTGGCTATGCTGAAGAAAATCCCTGGAGTTAAACGGGCTGCCACGGCGGAAGAGGTGTTGCCGGAGTATGGGGCGGTGAGAGGTGGGTTTCCGATGTGGGTTTCCGTTAAGGATCGGAGATTGTTGCAGGCGGCGGTGAATGAAACAAAGTTCAATTTGGTGGTGGCGAAGGATGGCTCCGGGAATTTCACCACTGTCGGCGAGGCTGTCGCCGCCGCTCCAAATTCGAGCACGACCAGGTTTGTAATATACATAAAAGCAGGGGCCTATTTTGAGAATGTGGAAATAGAACGGAAAAAGACAAACTTGGTGTTCGTCGGCGACGGGATCGGCAAGACGCTGATAAAGGCCAACCGGAATGTCGTTGACGGCTGGACTACCTTCCGATCAGCCACCGTCGGTTAGTTTCCGTTTCACTCTTTTTTTTTTTAATAAATTAATAATTAATTACTTTCATTAAAATGATGAGACCACCTGACGATCATCAGATCTTGCCTCTGAAATAATTTATTTATTTATTAATCATAATTTTGGATCTGTTCTAATATTTTAAAATTAGAAAACGTTTCTTAATTTAATGTGTTTTTTTTTTTAATTTTAGAAAATAAATAGGTATAGAATTGAAACTTAAAAAAGAAAATAAAAATTTAAAATAGAAAAGGGAAATCAGTAGATAATGGTTGAATATAAAATAGACTAAACTAATAAATAAATAAAAAAAATTAAATTTTAAAAATTTAATAATATTTTAAAATTTAAAAAAAAAAATTAAAAATTTAAAAACACTCTTGTTTTTTTGTGGCTGAAATTGTTAATATTTTATTTAAAAAATATCTTTTAATTTTGAGAAATTCCTGTCAAACTTTCAAAATTTCATTAATATAATTTTTAAACTTTTTTAAATGTCATTAAATATTTTAAAAATAATAATTGTAGTATCCATTTAAAAATATTAATAAACTTTTAAAAGTAACTCTAGATAATAGATGGTTTTGATTTATATATTGACGATAAATTTTTTTGAATTTTTTTATTTATAAAAATTAAGGGTTAAGGTTATAGGTGTTTTCAAATAAAAATAAAAATTAAAAGGTATTAATAAAAAAAAATTAATTCAAAAATATTTTTGAAATATTATTGATAGTTTGATAATTTTTAAAACATTTAAAATTATTATTGAATTTTTTGAAAATTTAAGGATGTTTTTTATTAATTATTTTGAAAGTGGCAGAAGGGTAGGGGAAGGCGAGTACAGTGGGCCCAGTGGGCCCAGTATGGCCCAAAAGCCGCTAATATGGGCGAACCGCATGTGGGTTGGATAAGATTGCCAATCTCCGGAAAAAAGGGCAGTTGCCTTTTTCAATCAATTTTTAAATTTATTCTATAAATTTATAAATGTTTTTTAAATCTACTTTTTTATTTGGTCTTATAATTTTTTATTTTTTTTTCGAGAATAATAACATATGTTAGTTACTATGTTCAGCCGCGGTCGGCAATGGGTTCATAGCGAAAGGCATAACATTCGAAAACTTTGCTGGTCCAAGCAAGCACCAAGCCGTAGCCTTCCGAAGCAACTCCGACTTCTCAGCATTTTACCAATGCAGCTTCATCGGCTATCAAGACACTCTCTACGTCCATTCTCTCCGTCAATTCTACCGCGAATGCGACGTCTATGGCACCGTCGACTTCATCTTCGGTAACGCTGCAGTCGTCTTCCAAAACTGCAATCTCTACGCTCGAAAACCCAACTCAAACCAACGCAACATATTCACAGCTCAAGGTCGTGAGGATCCTAACCAAAATACCGGAATCTCAATCCTCAATTGCAAGGTTGAAGCCGCCTCTGATTTGATCCCAGTTCTTCCCTCTTTCAGAACTTACTTAGGTCGACCCTGGAAGCTCTACTCGAGGACGGTTTTTCTCAAGTCATTTATTGGGCAGTTGGTTGAGCCAGTCGGATGGCTTGAGTGGAGTGGGACATTCGCATTAGATACTTTGTATTATGGGGAGTATTTGAATCGTGGGCCTGGTTCTAATACAACTGGACGAGTCACTTGGCCAGGTTATCGGGTCATTACTAACTCAACGGAAGCGAGTCAGTTTACGGTGGAGGCGTTTATACAAGGTAGTGAGTGGCTGAACTCGACCGAAATTCCTTTCTTCTCCGGCTTGGCT

mRNA sequence

AAATATTATAAATCTACTCTTCATCTTCCTTCTTGACAGCCACATAAATCTCCCCTTCATCTTCTTCCCTCCTCTCTCTGTTTCAGACATGTACGGCCTCAGAAACAGAGCTCAAAGCTCTTCAAATCCCACCGCCTCCATTTCCGCCATTTTCACCTTCTTTTCCGTTCTTATCCTTTGCTCCTTCAAACCCATTTCTTCAAATCCCCAAACCCACCAAACCGCTCGATCCCTTTGCAATGGCACTCTGTTTCCAAATCTCTGTTTCTCAAAGCTCTCCTCTTTTCCCCAACTCGCCTCCCTCTCGCCGGAAAAGCTCGCCGGCTCCGCCCTCAACTTCACCAACGACGAGGTTCACGGTGCCTACGCCAATTGCTCCAAGCTCAAGACTCTCTTCTACGGAGGTCTGAGTCCCACTGACCGCCGCGCCCTCGATGACTGCCTTGAGCTTCTCGATGGCAGCATTGCCGAACTCCAAACTTCGATTTACGATCTATCACCGTCGCAGTCCGCCGCCCGCCACTCCCACGATCTGTTGACGTTGGTCAGCGCCGCCATGACTAACCACCGGACTTGTCTTGATGGGTTCTATAATAGTTCCGGGACTGTGAGGAAACGGGTGGAGCTGTATTTGGGGAAAATCGAACAGCATTTGAGTATTGATTTGGCTATGCTGAAGAAAATCCCTGGAGTTAAACGGGCTGCCACGGCGGAAGAGGTGTTGCCGGAGTATGGGGCGGTGAGAGGTGGGTTTCCGATGTGGGTTTCCGTTAAGGATCGGAGATTGTTGCAGGCGGCGGTGAATGAAACAAAGTTCAATTTGGTGGTGGCGAAGGATGGCTCCGGGAATTTCACCACTGTCGGCGAGGCTGTCGCCGCCGCTCCAAATTCGAGCACGACCAGGTTTGTAATATACATAAAAGCAGGGGCCTATTTTGAGAATGTGGAAATAGAACGGAAAAAGACAAACTTGGTGTTCGTCGGCGACGGGATCGGCAAGACGCTGATAAAGGCCAACCGGAATGTCGTTGACGGCTGGACTACCTTCCGATCAGCCACCGTCGGTAGTGAGTGGCTGAACTCGACCGAAATTCCTTTCTTCTCCGGCTTGGCT

Coding sequence (CDS)

ATGTACGGCCTCAGAAACAGAGCTCAAAGCTCTTCAAATCCCACCGCCTCCATTTCCGCCATTTTCACCTTCTTTTCCGTTCTTATCCTTTGCTCCTTCAAACCCATTTCTTCAAATCCCCAAACCCACCAAACCGCTCGATCCCTTTGCAATGGCACTCTGTTTCCAAATCTCTGTTTCTCAAAGCTCTCCTCTTTTCCCCAACTCGCCTCCCTCTCGCCGGAAAAGCTCGCCGGCTCCGCCCTCAACTTCACCAACGACGAGGTTCACGGTGCCTACGCCAATTGCTCCAAGCTCAAGACTCTCTTCTACGGAGGTCTGAGTCCCACTGACCGCCGCGCCCTCGATGACTGCCTTGAGCTTCTCGATGGCAGCATTGCCGAACTCCAAACTTCGATTTACGATCTATCACCGTCGCAGTCCGCCGCCCGCCACTCCCACGATCTGTTGACGTTGGTCAGCGCCGCCATGACTAACCACCGGACTTGTCTTGATGGGTTCTATAATAGTTCCGGGACTGTGAGGAAACGGGTGGAGCTGTATTTGGGGAAAATCGAACAGCATTTGAGTATTGATTTGGCTATGCTGAAGAAAATCCCTGGAGTTAAACGGGCTGCCACGGCGGAAGAGGTGTTGCCGGAGTATGGGGCGGTGAGAGGTGGGTTTCCGATGTGGGTTTCCGTTAAGGATCGGAGATTGTTGCAGGCGGCGGTGAATGAAACAAAGTTCAATTTGGTGGTGGCGAAGGATGGCTCCGGGAATTTCACCACTGTCGGCGAGGCTGTCGCCGCCGCTCCAAATTCGAGCACGACCAGGTTTGTAATATACATAAAAGCAGGGGCCTATTTTGAGAATGTGGAAATAGAACGGAAAAAGACAAACTTGGTGTTCGTCGGCGACGGGATCGGCAAGACGCTGATAAAGGCCAACCGGAATGTCGTTGACGGCTGGACTACCTTCCGATCAGCCACCGTCGGTAGTGAGTGGCTGAACTCGACCGAAATTCCTTTCTTCTCCGGCTTGGCT

Protein sequence

MYGLRNRAQSSSNPTASISAIFTFFSVLILCSFKPISSNPQTHQTARSLCNGTLFPNLCFSKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLSPTDRRALDDCLELLDGSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVELYLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLVFVGDGIGKTLIKANRNVVDGWTTFRSATVGSEWLNSTEIPFFSGLA
Homology
[Close]
BLAST of Cp4.1LG12g05860 vs. Swiss-Prot
Match: PME40_ARATH (Probable pectinesterase/pectinesterase inhibitor 40 OS=Arabidopsis thaliana GN=PME40 PE=2 SV=1)

HSP 1 Score: 226.1 bits (575), Expect = 5.8e-58
Identity = 131/263 (49.81%), Postives = 168/263 (63.88%), Query Frame = 1 

Query: 76  KLAGSALNFTNDEVHGAYANCSKLKTLFYGGLSPTDRRALDDCLELLDGSIAELQTSIYD 135
           +L  +ALN T   V+ + +N S L       LS  D  A DDCLELLD ++ +L T+I  
Sbjct: 39  ELVVTALNQTISNVNLSSSNFSDLLQRLGSNLSHRDLCAFDDCLELLDDTVFDLTTAISK 98

Query: 136 LSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGT------------VRKRVELYLG 195
           L   +S +   H++  L+SAAMTN RTCLDGF +S               V + ++  L 
Sbjct: 99  L---RSHSPELHNVKMLLSAAMTNTRTCLDGFASSDNDENLNNNDNKTYGVAESLKESLF 158

Query: 196 KIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKF 255
            I  H+S  LAML+ IPG       E+V         GFPMWVS  DR LLQ  V+ETK 
Sbjct: 159 NISSHVSDSLAMLENIPGHIPGKVKEDV---------GFPMWVSGSDRNLLQDPVDETKV 218

Query: 256 NLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLVFVGDGIG 315
           NLVVA++G+GN+TT+GEA++AAPNSS TRFVIYIK G YFEN+EI R+KT ++F+GDGIG
Sbjct: 219 NLVVAQNGTGNYTTIGEAISAAPNSSETRFVIYIKCGEYFENIEIPREKTMIMFIGDGIG 278

Query: 316 KTLIKANRNVVDGWTTFRSATVG 327
           +T+IKANR+  DGWT F SATVG
Sbjct: 279 RTVIKANRSYADGWTAFHSATVG 289

[Close]
BLAST of Cp4.1LG12g05860 vs. Swiss-Prot
Match: PME2_ARATH (Pectinesterase 2 OS=Arabidopsis thaliana GN=PME2 PE=2 SV=2)

HSP 1 Score: 201.8 bits (512), Expect = 1.2e-50
Identity = 129/305 (42.30%), Postives = 175/305 (57.38%), Query Frame = 1 

Query: 42  THQTARSLCNGTLFPNLCFSKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKT 101
           +H   +S+C+ TL+P LCFS +++       S +++  ++LN T   V   Y    KL  
Sbjct: 58  SHAILKSVCSSTLYPELCFSAVAATGGKELTSQKEVIEASLNLTTKAVKHNYFAVKKLIA 117

Query: 102 LFYGGLSPTDRRALDDCLELLDGSIAELQTSIYDLS--PSQSAAR-HSHDLLTLVSAAMT 161
               GL+P +  AL DCLE +D ++ EL  ++ DL   P Q + R H+ DL TL+S+A+T
Sbjct: 118 K-RKGLTPREVTALHDCLETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAIT 177

Query: 162 NHRTCLDGFYNSSGTVRKRVELYLGKI--EQHLSIDLAMLKKIPGV---------KRAAT 221
           N  TCLDGF       + R  L  G++  E   S  LAM+K +            K +  
Sbjct: 178 NQGTCLDGFSYDDADRKVRKALLKGQVHVEHMCSNALAMIKNMTETDIANFELRDKSSTF 237

Query: 222 AEEVLPEYGAVRG-----GFPMWVSVKDRRLLQAAVNETKFNLVVAKDGSGNFTTVGEAV 281
                 +   V G     G+P W+SV DRRLLQ +    K +  VA DGSG+FTTV  AV
Sbjct: 238 TNNNNRKLKEVTGDLDSDGWPKWLSVGDRRLLQGST--IKADATVADDGSGDFTTVAAAV 297

Query: 282 AAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLVFVGDGIGKTLIKANRNVVDGWTTFRS 328
           AAAP  S  RFVI+IKAG Y ENVE+ +KKTN++F+GDG GKT+I  +RNVVDG TTF S
Sbjct: 298 AAAPEKSNKRFVIHIKAGVYRENVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGSTTFHS 357

[Close]
BLAST of Cp4.1LG12g05860 vs. Swiss-Prot
Match: PME39_ARATH (Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana GN=PME39 PE=2 SV=1)

HSP 1 Score: 200.7 bits (509), Expect = 2.6e-50
Identity = 115/253 (45.45%), Postives = 161/253 (63.64%), Query Frame = 1 

Query: 82  LNFTNDEVHGAYANCSKLKTLFYGGLSPTDRRALDDCLELLDGSIAELQTSIYDLSPSQS 141
           LN T  +V+ A +N S L+T  +  L+  +R A +DCL LLD +I++L+T++ DL   +S
Sbjct: 61  LNLTILKVNLASSNFSDLQTRLFPNLTHYERCAFEDCLGLLDDTISDLETAVSDL---RS 120

Query: 142 AARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVELYLGK--------IEQHLSIDL 201
           ++   +D+  L++  MT   TCLDGF  S       +   L +        I  +LS  L
Sbjct: 121 SSLEFNDISMLLTNVMTYQDTCLDGFSTSDNENNNDMTYELPENLKEIILDISNNLSNSL 180

Query: 202 AMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKFNLVVAKDGSG 261
            ML+ I   K +  + EV  EY       P W+S  D+RLL+A V ET +NL VA DG+G
Sbjct: 181 HMLQVISRKKPSPKSSEVDVEY-------PSWLSENDQRLLEAPVQETNYNLSVAIDGTG 240

Query: 262 NFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLVFVGDGIGKTLIKANRNV 321
           NFTT+ +AV AAPN S TRF+IYIK G YFENVE+ +KKT ++F+GDGIGKT+IKANR+ 
Sbjct: 241 NFTTINDAVFAAPNMSETRFIIYIKGGEYFENVELPKKKTMIMFIGDGIGKTVIKANRSR 300

Query: 322 VDGWTTFRSATVG 327
           +DGW+TF++ TVG
Sbjct: 301 IDGWSTFQTPTVG 303

[Close]
BLAST of Cp4.1LG12g05860 vs. Swiss-Prot
Match: PME3_CITSI (Pectinesterase 3 OS=Citrus sinensis PE=1 SV=1)

HSP 1 Score: 198.7 bits (504), Expect = 1.0e-49
Identity = 122/300 (40.67%), Postives = 176/300 (58.67%), Query Frame = 1 

Query: 37  SSNPQTHQTARSLCNGTLFPNLCFSKLSSFPQLAS--LSPEKLAGSALNFTNDEVHGAYA 96
           + N   H   +S C+ T +P+LCFS +++ P+ +    S + +   +LN T   V   Y 
Sbjct: 60  NGNEPHHAILKSSCSSTRYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYF 119

Query: 97  NCSKLKTLFYGGLSPTDRRALDDCLELLDGSIAELQTSIYDLSP---SQSAARHSHDLLT 156
              KL  L    L+  ++ AL DCLE +D ++ EL  ++ DL      +S ++H+ DL T
Sbjct: 120 GIQKL--LKRTNLTKREKVALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKT 179

Query: 157 LVSAAMTNHRTCLDGFYNSSGTVRKRVELYLGKI--EQHLSIDLAMLKKIPG----VKRA 216
           L+SAAMTN  TCLDGF +       R  L  G++  E+  S  LAM+K +      + R 
Sbjct: 180 LMSAAMTNQGTCLDGFSHDDANKHVRDALSDGQVHVEKMCSNALAMIKNMTDTDMMIMRT 239

Query: 217 ATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKFNLVVAKDGSGNFTTVGEAVAAA 276
           +   +++ E   V  G+P W+S  DRRLLQ++      N+VVA DGSGNF TV  +VAAA
Sbjct: 240 SNNRKLIEETSTV-DGWPAWLSTGDRRLLQSS--SVTPNVVVAADGSGNFKTVAASVAAA 299

Query: 277 PNSSTTRFVIYIKAGAYFENVEIERKKTNLVFVGDGIGKTLIKANRNVVDGWTTFRSATV 326
           P   T R++I IKAG Y ENVE+ +K  N++F+GDG  +T+I  +RNVVDG TTF+SATV
Sbjct: 300 PQGGTKRYIIRIKAGVYRENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATV 354

[Close]
BLAST of Cp4.1LG12g05860 vs. Swiss-Prot
Match: PME1_CITSI (Pectinesterase 1 OS=Citrus sinensis GN=PECS-1.1 PE=2 SV=1)

HSP 1 Score: 195.7 bits (496), Expect = 8.5e-49
Identity = 122/299 (40.80%), Postives = 173/299 (57.86%), Query Frame = 1 

Query: 37  SSNPQTHQTARSLCNGTLFPNLCFSKLSSFPQLAS--LSPEKLAGSALNFTNDEVHGAYA 96
           + N   H   +S C+ T +P+LCFS +++ P+ +    S + +   +LN T   V   Y 
Sbjct: 60  NGNEPHHAILKSSCSSTRYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYF 119

Query: 97  NCSKLKTLFYGGLSPTDRRALDDCLELLDGSIAELQTSIYDLSP---SQSAARHSHDLLT 156
              KL  L    L+  ++ AL DCLE +D ++ EL  ++ DL      +S ++H+ DL T
Sbjct: 120 GIQKL--LKRTNLTKREKVALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKT 179

Query: 157 LVSAAMTNHRTCLDGFYNSSGTVRKRVELYLGKI--EQHLSIDLAMLKKIPG----VKRA 216
           L+SAAMTN  TCLDGF +       R  L  G++  E+  S  LAM+K +      + R 
Sbjct: 180 LMSAAMTNQGTCLDGFSHDDANKHVRDALSDGQVHVEKMCSNALAMIKNMTDTDMMIMRT 239

Query: 217 ATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKFNLVVAKDGSGNFTTVGEAVAAA 276
           +   ++  E   V  G+P W+S  DRRLLQ++      N VVA DGSGNF TV  AVAAA
Sbjct: 240 SNNRKLTEETSTV-DGWPAWLSPGDRRLLQSS--SVTPNAVVAADGSGNFKTVAAAVAAA 299

Query: 277 PNSSTTRFVIYIKAGAYFENVEIERKKTNLVFVGDGIGKTLIKANRNVVDGWTTFRSAT 325
           P   T R++I IKAG Y ENVE+ +K  N++F+GDG  +T+I  +RNVVDG TTF+SAT
Sbjct: 300 PQGGTKRYIIRIKAGVYRENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSAT 353

[Close]
BLAST of Cp4.1LG12g05860 vs. TrEMBL
Match: A0A0A0K5K8_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_7G405320 PE=4 SV=1)

HSP 1 Score: 483.4 bits (1243), Expect = 2.3e-133
Identity = 257/329 (78.12%), Postives = 280/329 (85.11%), Query Frame = 1 

Query: 8   AQSSSNPTASISAIFTFFSVLILCSFKPISSNP---------QT-HQTARSLCNGTLFPN 67
           + SSS+ ++S S+  +  S     S    SS+P         QT HQTARSLCNGTLFP+
Sbjct: 53  SSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSPPPPPPLPLFQTFHQTARSLCNGTLFPD 112

Query: 68  LCFSKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLSPTDRRALDD 127
           LCFSKLSSFPQLASLSPEKLAGSALNFT+ EV  AY NC+ LKT  Y GL+PTDR ALDD
Sbjct: 113 LCFSKLSSFPQLASLSPEKLAGSALNFTSREVLLAYTNCTNLKTHLYSGLNPTDRHALDD 172

Query: 128 CLELLDGSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKR 187
           CLELLDGSIAEL+ SI+DL+PSQS A HSHDLLTLVSAAMTNHRTC+DGFYNSSGTVR R
Sbjct: 173 CLELLDGSIAELKASIFDLAPSQSPALHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSR 232

Query: 188 VELYLGKIEQHLSIDLAMLKKIPGVKRA-ATAEEVLPEYGAVRGGFPMWVSVKDRRLLQA 247
           VELYLGKI QHLSIDLAMLKKIPGV RA    +E+LPEYGAV+GGFP WVSVKDRRLLQA
Sbjct: 233 VELYLGKIGQHLSIDLAMLKKIPGVNRATGVDQEMLPEYGAVKGGFPKWVSVKDRRLLQA 292

Query: 248 AVNETKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLV 307
           AVNETKFN+VVAKDGSGNFTTV EAVAAAPN+STTRFVIYIKAGAYFENVEI R K+NL+
Sbjct: 293 AVNETKFNMVVAKDGSGNFTTVSEAVAAAPNASTTRFVIYIKAGAYFENVEIGRAKSNLM 352

Query: 308 FVGDGIGKTLIKANRNVVDGWTTFRSATV 326
           FVGDGIGKTLIKA+RNVVDGWTTFRSATV
Sbjct: 353 FVGDGIGKTLIKADRNVVDGWTTFRSATV 381

[Close]
BLAST of Cp4.1LG12g05860 vs. TrEMBL
Match: V7BH80_PHAVU (Pectinesterase OS=Phaseolus vulgaris GN=PHAVU_007G218500g PE=4 SV=1)

HSP 1 Score: 340.5 bits (872), Expect = 2.4e-90
Identity = 181/319 (56.74%), Postives = 227/319 (71.16%), Query Frame = 1 

Query: 17  SISAIFTFFSVLILCSFK-----PISSNPQTH-----QTARSLCNGTLFPNLCFSKLSSF 76
           ++++I T  S L+  S         SS    H     Q A+S C GTL+P+LC S L++F
Sbjct: 5   TLASILTVISALLFLSTHFTNTPTRSSEKHLHFHKHMQVAQSTCQGTLYPDLCVSTLATF 64

Query: 77  PQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLSPTDRRALDDCLELLDGSI 136
           P L S S  ++  S +N T  EV  ++ NCS LK      L+P D+RALDDCL+L   + 
Sbjct: 65  PDLTSKSVPQVISSVVNHTMYEVKSSFYNCSSLKKKIRN-LNPLDQRALDDCLKLFQDTN 124

Query: 137 AELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVELYLGKIE 196
            EL+T+I DLS S   ++  HDL TL+S AMTN  TCLDGF  S G VR R+E  L +I 
Sbjct: 125 LELKTTIDDLSKSSIGSKRHHDLQTLLSGAMTNLYTCLDGFAYSKGRVRDRIEKKLLEIS 184

Query: 197 QHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKFNLV 256
            H+S  LAMLKK+PGVK   +  EV PEYG ++ GFP+WVS KDR+LLQA+VNE+KF+LV
Sbjct: 185 HHMSNSLAMLKKVPGVKTRTSKSEVFPEYGKMKKGFPLWVSPKDRKLLQASVNESKFDLV 244

Query: 257 VAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLVFVGDGIGKTL 316
           VAKDG+GNFTT+GEA+A APNSSTTRFVI+IK GAYFENVE+ RKKTNL+FVGDGIGKT+
Sbjct: 245 VAKDGTGNFTTIGEALALAPNSSTTRFVIHIKEGAYFENVEVIRKKTNLMFVGDGIGKTV 304

Query: 317 IKANRNVVDGWTTFRSATV 326
           +K +RNVVDGWTTF+SATV
Sbjct: 305 VKGSRNVVDGWTTFQSATV 322

[Close]
BLAST of Cp4.1LG12g05860 vs. TrEMBL
Match: A0A0L9VH61_PHAAN (Pectinesterase OS=Phaseolus angularis GN=LR48_Vigan10g027800 PE=4 SV=1)

HSP 1 Score: 333.2 bits (853), Expect = 3.8e-88
Identity = 170/282 (60.28%), Postives = 211/282 (74.82%), Query Frame = 1 

Query: 44  QTARSLCNGTLFPNLCFSKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLF 103
           Q A+S C GTL+P LC S L++FP L S S  +L  S +N T  EV  ++ NCS LK   
Sbjct: 42  QVAQSTCQGTLYPELCVSTLATFPDLTSKSLPQLISSVVNHTIYEVKSSFYNCSSLKKKI 101

Query: 104 YGGLSPTDRRALDDCLELLDGSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTC 163
              L+P D+RALDDCL+L + +  EL+ +I DLS S   ++  HDL TL+S AMTN  TC
Sbjct: 102 RN-LNPLDQRALDDCLKLFEDTNLELKATIDDLSKSTIGSKRHHDLQTLLSGAMTNLYTC 161

Query: 164 LDGFYNSSGTVRKRVELYLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFP 223
           LDGF  S G VR R+E  L +I  H+S  LAMLKK+P VK+  +  EV PEYG ++ GFP
Sbjct: 162 LDGFAYSKGRVRDRIEKKLLEISHHMSNSLAMLKKVPEVKKTTSKSEVFPEYGKMKKGFP 221

Query: 224 MWVSVKDRRLLQAAVNETKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYF 283
            WVS  DR+LLQA+V+ETKF+LVVAKDG+GNFTT+GEA+A APNSSTTRFVI+IK GAYF
Sbjct: 222 SWVSPNDRKLLQASVDETKFDLVVAKDGTGNFTTIGEALAVAPNSSTTRFVIHIKEGAYF 281

Query: 284 ENVEIERKKTNLVFVGDGIGKTLIKANRNVVDGWTTFRSATV 326
           ENVE+ RK+TNL+FVGDGIGKT++K +RNVVDGWTTF+SATV
Sbjct: 282 ENVEVIRKQTNLMFVGDGIGKTVVKGSRNVVDGWTTFQSATV 322

[Close]
BLAST of Cp4.1LG12g05860 vs. TrEMBL
Match: A0A0S3SSE1_PHAAN (Pectinesterase OS=Vigna angularis var. angularis GN=Vigan.08G252900 PE=4 SV=1)

HSP 1 Score: 333.2 bits (853), Expect = 3.8e-88
Identity = 170/282 (60.28%), Postives = 211/282 (74.82%), Query Frame = 1 

Query: 44  QTARSLCNGTLFPNLCFSKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLF 103
           Q A+S C GTL+P LC S L++FP L S S  +L  S +N T  EV  ++ NCS LK   
Sbjct: 42  QVAQSTCQGTLYPELCVSTLATFPDLTSKSLPQLISSVVNHTIYEVKSSFYNCSSLKKKI 101

Query: 104 YGGLSPTDRRALDDCLELLDGSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTC 163
              L+P D+RALDDCL+L + +  EL+ +I DLS S   ++  HDL TL+S AMTN  TC
Sbjct: 102 RN-LNPLDQRALDDCLKLFEDTNLELKATIDDLSKSTIGSKRHHDLQTLLSGAMTNLYTC 161

Query: 164 LDGFYNSSGTVRKRVELYLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFP 223
           LDGF  S G VR R+E  L +I  H+S  LAMLKK+P VK+  +  EV PEYG ++ GFP
Sbjct: 162 LDGFAYSKGRVRDRIEKKLLEISHHMSNSLAMLKKVPEVKKTTSKSEVFPEYGKMKKGFP 221

Query: 224 MWVSVKDRRLLQAAVNETKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYF 283
            WVS  DR+LLQA+V+ETKF+LVVAKDG+GNFTT+GEA+A APNSSTTRFVI+IK GAYF
Sbjct: 222 SWVSPNDRKLLQASVDETKFDLVVAKDGTGNFTTIGEALAVAPNSSTTRFVIHIKEGAYF 281

Query: 284 ENVEIERKKTNLVFVGDGIGKTLIKANRNVVDGWTTFRSATV 326
           ENVE+ RK+TNL+FVGDGIGKT++K +RNVVDGWTTF+SATV
Sbjct: 282 ENVEVIRKQTNLMFVGDGIGKTVVKGSRNVVDGWTTFQSATV 322

[Close]
BLAST of Cp4.1LG12g05860 vs. TrEMBL
Match: K7K5X8_SOYBN (Pectinesterase OS=Glycine max GN=GLYMA_02G016500 PE=4 SV=1)

HSP 1 Score: 330.1 bits (845), Expect = 3.2e-87
Identity = 169/281 (60.14%), Postives = 213/281 (75.80%), Query Frame = 1 

Query: 46  ARSLCNGTLFPNLCFSKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYG 105
           A+S C GTL+P+LC S L++FP LA+ S  ++  S +N T  EV  +  NCS LK +   
Sbjct: 52  AKSTCQGTLYPDLCVSTLATFPDLATKSVPQVISSVVNHTMYEVRSSSYNCSGLKKMLKN 111

Query: 106 GLSPTDRRALDDCLELLDGSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLD 165
            L+P D+RALDDCL+L + +  EL+ +I DLS S   ++  HDL T++S AMTN  TCLD
Sbjct: 112 -LNPLDQRALDDCLKLFEDTNVELKATIDDLSKSTIGSKRHHDLQTMLSGAMTNLYTCLD 171

Query: 166 GFYNSSGTVRKRVELYLGKIEQHLSIDLAMLKKIPGVKRAATAEEVL-PEYGAVRGGFPM 225
           GF  S G VR R+E  L +I  H+S  LAML K+PGVK+  T+E V+ PEYG ++ GFP 
Sbjct: 172 GFAYSKGRVRDRIEKKLLEISHHVSNSLAMLNKVPGVKKLTTSESVVFPEYGNMKKGFPS 231

Query: 226 WVSVKDRRLLQAAVNETKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFE 285
           WVS KDR+LLQA V ETKF+L+VAKDG+GNFTT+GEA+A APNSSTTRFVI+IK GAYFE
Sbjct: 232 WVSSKDRKLLQAKVKETKFDLLVAKDGTGNFTTIGEALAVAPNSSTTRFVIHIKEGAYFE 291

Query: 286 NVEIERKKTNLVFVGDGIGKTLIKANRNVVDGWTTFRSATV 326
           NVE+ RKKTNL+FVGDGIGKT++K +RNVVDGWTTF+SATV
Sbjct: 292 NVEVIRKKTNLMFVGDGIGKTVVKGSRNVVDGWTTFQSATV 331

[Close]
BLAST of Cp4.1LG12g05860 vs. TAIR10
Match: AT4G02320.1 (AT4G02320.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 226.1 bits (575), Expect = 3.3e-59
Identity = 131/263 (49.81%), Postives = 168/263 (63.88%), Query Frame = 1 

Query: 76  KLAGSALNFTNDEVHGAYANCSKLKTLFYGGLSPTDRRALDDCLELLDGSIAELQTSIYD 135
           +L  +ALN T   V+ + +N S L       LS  D  A DDCLELLD ++ +L T+I  
Sbjct: 39  ELVVTALNQTISNVNLSSSNFSDLLQRLGSNLSHRDLCAFDDCLELLDDTVFDLTTAISK 98

Query: 136 LSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGT------------VRKRVELYLG 195
           L   +S +   H++  L+SAAMTN RTCLDGF +S               V + ++  L 
Sbjct: 99  L---RSHSPELHNVKMLLSAAMTNTRTCLDGFASSDNDENLNNNDNKTYGVAESLKESLF 158

Query: 196 KIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKF 255
            I  H+S  LAML+ IPG       E+V         GFPMWVS  DR LLQ  V+ETK 
Sbjct: 159 NISSHVSDSLAMLENIPGHIPGKVKEDV---------GFPMWVSGSDRNLLQDPVDETKV 218

Query: 256 NLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLVFVGDGIG 315
           NLVVA++G+GN+TT+GEA++AAPNSS TRFVIYIK G YFEN+EI R+KT ++F+GDGIG
Sbjct: 219 NLVVAQNGTGNYTTIGEAISAAPNSSETRFVIYIKCGEYFENIEIPREKTMIMFIGDGIG 278

Query: 316 KTLIKANRNVVDGWTTFRSATVG 327
           +T+IKANR+  DGWT F SATVG
Sbjct: 279 RTVIKANRSYADGWTAFHSATVG 289

[Close]
BLAST of Cp4.1LG12g05860 vs. TAIR10
Match: AT1G53830.1 (AT1G53830.1 pectin methylesterase 2)

HSP 1 Score: 201.8 bits (512), Expect = 6.6e-52
Identity = 129/305 (42.30%), Postives = 175/305 (57.38%), Query Frame = 1 

Query: 42  THQTARSLCNGTLFPNLCFSKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKT 101
           +H   +S+C+ TL+P LCFS +++       S +++  ++LN T   V   Y    KL  
Sbjct: 58  SHAILKSVCSSTLYPELCFSAVAATGGKELTSQKEVIEASLNLTTKAVKHNYFAVKKLIA 117

Query: 102 LFYGGLSPTDRRALDDCLELLDGSIAELQTSIYDLS--PSQSAAR-HSHDLLTLVSAAMT 161
               GL+P +  AL DCLE +D ++ EL  ++ DL   P Q + R H+ DL TL+S+A+T
Sbjct: 118 K-RKGLTPREVTALHDCLETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAIT 177

Query: 162 NHRTCLDGFYNSSGTVRKRVELYLGKI--EQHLSIDLAMLKKIPGV---------KRAAT 221
           N  TCLDGF       + R  L  G++  E   S  LAM+K +            K +  
Sbjct: 178 NQGTCLDGFSYDDADRKVRKALLKGQVHVEHMCSNALAMIKNMTETDIANFELRDKSSTF 237

Query: 222 AEEVLPEYGAVRG-----GFPMWVSVKDRRLLQAAVNETKFNLVVAKDGSGNFTTVGEAV 281
                 +   V G     G+P W+SV DRRLLQ +    K +  VA DGSG+FTTV  AV
Sbjct: 238 TNNNNRKLKEVTGDLDSDGWPKWLSVGDRRLLQGST--IKADATVADDGSGDFTTVAAAV 297

Query: 282 AAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLVFVGDGIGKTLIKANRNVVDGWTTFRS 328
           AAAP  S  RFVI+IKAG Y ENVE+ +KKTN++F+GDG GKT+I  +RNVVDG TTF S
Sbjct: 298 AAAPEKSNKRFVIHIKAGVYRENVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGSTTFHS 357

[Close]
BLAST of Cp4.1LG12g05860 vs. TAIR10
Match: AT4G02300.1 (AT4G02300.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 200.7 bits (509), Expect = 1.5e-51
Identity = 115/253 (45.45%), Postives = 161/253 (63.64%), Query Frame = 1 

Query: 82  LNFTNDEVHGAYANCSKLKTLFYGGLSPTDRRALDDCLELLDGSIAELQTSIYDLSPSQS 141
           LN T  +V+ A +N S L+T  +  L+  +R A +DCL LLD +I++L+T++ DL   +S
Sbjct: 61  LNLTILKVNLASSNFSDLQTRLFPNLTHYERCAFEDCLGLLDDTISDLETAVSDL---RS 120

Query: 142 AARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVELYLGK--------IEQHLSIDL 201
           ++   +D+  L++  MT   TCLDGF  S       +   L +        I  +LS  L
Sbjct: 121 SSLEFNDISMLLTNVMTYQDTCLDGFSTSDNENNNDMTYELPENLKEIILDISNNLSNSL 180

Query: 202 AMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKFNLVVAKDGSG 261
            ML+ I   K +  + EV  EY       P W+S  D+RLL+A V ET +NL VA DG+G
Sbjct: 181 HMLQVISRKKPSPKSSEVDVEY-------PSWLSENDQRLLEAPVQETNYNLSVAIDGTG 240

Query: 262 NFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLVFVGDGIGKTLIKANRNV 321
           NFTT+ +AV AAPN S TRF+IYIK G YFENVE+ +KKT ++F+GDGIGKT+IKANR+ 
Sbjct: 241 NFTTINDAVFAAPNMSETRFIIYIKGGEYFENVELPKKKTMIMFIGDGIGKTVIKANRSR 300

Query: 322 VDGWTTFRSATVG 327
           +DGW+TF++ TVG
Sbjct: 301 IDGWSTFQTPTVG 303

[Close]
BLAST of Cp4.1LG12g05860 vs. TAIR10
Match: AT3G14310.1 (AT3G14310.1 pectin methylesterase 3)

HSP 1 Score: 186.8 bits (473), Expect = 2.2e-47
Identity = 120/316 (37.97%), Postives = 170/316 (53.80%), Query Frame = 1 

Query: 39  NPQTHQTARSLCNGTLFPNLCFSKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSK 98
           +P +H   RS C+ T +P LC S + +   +   S + +  +++N T   V   Y    K
Sbjct: 52  SPSSHAVLRSSCSSTRYPELCISAVVTAGGVELTSQKDVIEASVNLTITAVEHNYFTVKK 111

Query: 99  LKTLFYGGLSPTDRRALDDCLELLDGSIAELQTSIYDLS--PSQSAAR-HSHDLLTLVSA 158
           L      GL+P ++ AL DCLE +D ++ EL  ++ DL   P++   R H+ DL TL+S+
Sbjct: 112 LIKK-RKGLTPREKTALHDCLETIDETLDELHETVEDLHLYPTKKTLREHAGDLKTLISS 171

Query: 159 AMTNHRTCLDGFYNSSGTVRKRVELYLGKI--EQHLSIDLAMLKKIPGV----------- 218
           A+TN  TCLDGF +     + R  L  G+I  E   S  LAM+K +              
Sbjct: 172 AITNQETCLDGFSHDDADKQVRKALLKGQIHVEHMCSNALAMIKNMTDTDIANFEQKAKI 231

Query: 219 -----------KRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKFNLVVAKDG 278
                      +    A ++         G+P W+S  DRRLLQ +    K +  VA DG
Sbjct: 232 TSNNRKLKEENQETTVAVDIAGAGELDSEGWPTWLSAGDRRLLQGS--GVKADATVAADG 291

Query: 279 SGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLVFVGDGIGKTLIKANR 328
           SG F TV  AVAAAP +S  R+VI+IKAG Y ENVE+ +KK N++F+GDG  +T+I  +R
Sbjct: 292 SGTFKTVAAAVAAAPENSNKRYVIHIKAGVYRENVEVAKKKKNIMFMGDGRTRTIITGSR 351

[Close]
BLAST of Cp4.1LG12g05860 vs. TAIR10
Match: AT5G53370.1 (AT5G53370.1 pectin methylesterase PCR fragment F)

HSP 1 Score: 161.8 bits (408), Expect = 7.6e-40
Identity = 104/293 (35.49%), Postives = 150/293 (51.19%), Query Frame = 1 

Query: 44  QTARSLCNGTLFPNLCFSKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLF 103
           Q     C+ +L+PNLC   L  FP   +    +L   + N T  +   A    S   T+ 
Sbjct: 73  QAISRTCSKSLYPNLCIDTLLDFPGSLTADENELIHISFNATLQKFSKALYTSS---TIT 132

Query: 104 YGGLSPTDRRALDDCLELLDGSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTC 163
           Y  + P  R A D CLELLD S+  L  ++  +        HS D++T +S+AMTNH TC
Sbjct: 133 YTQMPPRVRSAYDSCLELLDDSVDALTRALSSVVVVSGDESHS-DVMTWLSSAMTNHDTC 192

Query: 164 LDGFYNSSGTVRKRVELYLGKIEQH-------LSIDLAMLKKIPGV----KRAATAEEVL 223
            DGF    G   +  +  +G ++         L+I    +K + GV     R     E  
Sbjct: 193 TDGFDEIEGQGGEVKDQVIGAVKDLSEMVSNCLAIFAGKVKDLSGVPVVNNRKLLGTEET 252

Query: 224 PEYGAVRGGFPMWVSVKDRRLLQAAVNETKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTR 283
            E        P W+  +DR LL    +  + ++ V+KDGSG F T+ EA+  AP  S+ R
Sbjct: 253 EE-------LPNWLKREDRELLGTPTSAIQADITVSKDGSGTFKTIAEAIKKAPEHSSRR 312

Query: 284 FVIYIKAGAY-FENVEIERKKTNLVFVGDGIGKTLIKANRNVVDGWTTFRSAT 325
           FVIY+KAG Y  EN+++ RKKTNL+F+GDG GKT+I   +++ D  TTF +AT
Sbjct: 313 FVIYVKAGRYEEENLKVGRKKTNLMFIGDGKGKTVITGGKSIADDLTTFHTAT 354

[Close]
BLAST of Cp4.1LG12g05860 vs. NCBI nr
Match: gi|659101319|ref|XP_008451545.1| (PREDICTED: pectinesterase-like [Cucumis melo])

HSP 1 Score: 544.7 bits (1402), Expect = 1.2e-151
Identity = 282/325 (86.77%), Postives = 291/325 (89.54%), Query Frame = 1 

Query: 1   MYGLRNRAQSSSNPTASISAIFTFFSVLILCSFKPISSNPQTHQTARSLCNGTLFPNLCF 60
           MYGLRNRAQ SSNPT SIS I TFF +  L S KPISSNPQ HQTARSLCNGTLFPNLCF
Sbjct: 39  MYGLRNRAQISSNPT-SISTISTFFFIFFLFSSKPISSNPQIHQTARSLCNGTLFPNLCF 98

Query: 61  SKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLSPTDRRALDDCLE 120
           SKLSSFPQLASLSPEKLAGSALNFT  EV  AYANC+ LKT  Y GL+PTDR ALDDCLE
Sbjct: 99  SKLSSFPQLASLSPEKLAGSALNFTTREVLLAYANCTNLKTHLYCGLNPTDRHALDDCLE 158

Query: 121 LLDGSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVEL 180
           LLD SIAEL+ SI DL PSQSAARHSHDLLTLVSAAMTNHRTC+DGFYNSSGTVR RVEL
Sbjct: 159 LLDDSIAELKASILDLGPSQSAARHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRVEL 218

Query: 181 YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNE 240
           YLGKI QHLSIDLAMLKKIPGV RA   EEVLPEYGAV+GGFP WVSVKDRRLLQAAVNE
Sbjct: 219 YLGKIGQHLSIDLAMLKKIPGVNRATKVEEVLPEYGAVKGGFPKWVSVKDRRLLQAAVNE 278

Query: 241 TKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLVFVGD 300
           TKFNLVVAKDGSGNFTTV EAVAAAPNSSTTRFVIYIKAGAYFENVEI R K+NL+FVGD
Sbjct: 279 TKFNLVVAKDGSGNFTTVNEAVAAAPNSSTTRFVIYIKAGAYFENVEIGRAKSNLMFVGD 338

Query: 301 GIGKTLIKANRNVVDGWTTFRSATV 326
           GIGKTLIK +RNVVDGWTTFRSATV
Sbjct: 339 GIGKTLIKGDRNVVDGWTTFRSATV 362

[Close]
BLAST of Cp4.1LG12g05860 vs. NCBI nr
Match: gi|449436467|ref|XP_004136014.1| (PREDICTED: pectinesterase-like [Cucumis sativus])

HSP 1 Score: 535.0 bits (1377), Expect = 9.4e-149
Identity = 275/328 (83.84%), Postives = 294/328 (89.63%), Query Frame = 1 

Query: 1   MYGLRNRAQSSSNPTA--SISAIFTFFSVLILCSFKPISSNPQTHQTARSLCNGTLFPNL 60
           MYGLRNRAQ SSNPT+  +IS I  FF +  L SFKPISSNPQ HQTARSLCNGTLFP+L
Sbjct: 1   MYGLRNRAQISSNPTSISTISTISPFFFIFFLFSFKPISSNPQVHQTARSLCNGTLFPDL 60

Query: 61  CFSKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLSPTDRRALDDC 120
           CFSKLSSFPQLASLSPEKLAGSALNFT+ EV  AY NC+ LKT  Y GL+PTDR ALDDC
Sbjct: 61  CFSKLSSFPQLASLSPEKLAGSALNFTSREVLLAYTNCTNLKTHLYSGLNPTDRHALDDC 120

Query: 121 LELLDGSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRV 180
           LELLDGSIAEL+ SI+DL+PSQS A HSHDLLTLVSAAMTNHRTC+DGFYNSSGTVR RV
Sbjct: 121 LELLDGSIAELKASIFDLAPSQSPALHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRV 180

Query: 181 ELYLGKIEQHLSIDLAMLKKIPGVKRA-ATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAA 240
           ELYLGKI QHLSIDLAMLKKIPGV RA    +E+LPEYGAV+GGFP WVSVKDRRLLQAA
Sbjct: 181 ELYLGKIGQHLSIDLAMLKKIPGVNRATGVDQEMLPEYGAVKGGFPKWVSVKDRRLLQAA 240

Query: 241 VNETKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLVF 300
           VNETKFN+VVAKDGSGNFTTV EAVAAAPN+STTRFVIYIKAGAYFENVEI R K+NL+F
Sbjct: 241 VNETKFNMVVAKDGSGNFTTVSEAVAAAPNASTTRFVIYIKAGAYFENVEIGRAKSNLMF 300

Query: 301 VGDGIGKTLIKANRNVVDGWTTFRSATV 326
           VGDGIGKTLIKA+RNVVDGWTTFRSATV
Sbjct: 301 VGDGIGKTLIKADRNVVDGWTTFRSATV 328

[Close]
BLAST of Cp4.1LG12g05860 vs. NCBI nr
Match: gi|700189742|gb|KGN44975.1| (Pectinesterase [Cucumis sativus])

HSP 1 Score: 483.4 bits (1243), Expect = 3.2e-133
Identity = 257/329 (78.12%), Postives = 280/329 (85.11%), Query Frame = 1 

Query: 8   AQSSSNPTASISAIFTFFSVLILCSFKPISSNP---------QT-HQTARSLCNGTLFPN 67
           + SSS+ ++S S+  +  S     S    SS+P         QT HQTARSLCNGTLFP+
Sbjct: 53  SSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSPPPPPPLPLFQTFHQTARSLCNGTLFPD 112

Query: 68  LCFSKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLSPTDRRALDD 127
           LCFSKLSSFPQLASLSPEKLAGSALNFT+ EV  AY NC+ LKT  Y GL+PTDR ALDD
Sbjct: 113 LCFSKLSSFPQLASLSPEKLAGSALNFTSREVLLAYTNCTNLKTHLYSGLNPTDRHALDD 172

Query: 128 CLELLDGSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKR 187
           CLELLDGSIAEL+ SI+DL+PSQS A HSHDLLTLVSAAMTNHRTC+DGFYNSSGTVR R
Sbjct: 173 CLELLDGSIAELKASIFDLAPSQSPALHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSR 232

Query: 188 VELYLGKIEQHLSIDLAMLKKIPGVKRA-ATAEEVLPEYGAVRGGFPMWVSVKDRRLLQA 247
           VELYLGKI QHLSIDLAMLKKIPGV RA    +E+LPEYGAV+GGFP WVSVKDRRLLQA
Sbjct: 233 VELYLGKIGQHLSIDLAMLKKIPGVNRATGVDQEMLPEYGAVKGGFPKWVSVKDRRLLQA 292

Query: 248 AVNETKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLV 307
           AVNETKFN+VVAKDGSGNFTTV EAVAAAPN+STTRFVIYIKAGAYFENVEI R K+NL+
Sbjct: 293 AVNETKFNMVVAKDGSGNFTTVSEAVAAAPNASTTRFVIYIKAGAYFENVEIGRAKSNLM 352

Query: 308 FVGDGIGKTLIKANRNVVDGWTTFRSATV 326
           FVGDGIGKTLIKA+RNVVDGWTTFRSATV
Sbjct: 353 FVGDGIGKTLIKADRNVVDGWTTFRSATV 381

[Close]
BLAST of Cp4.1LG12g05860 vs. NCBI nr
Match: gi|593689158|ref|XP_007145198.1| (hypothetical protein PHAVU_007G218500g [Phaseolus vulgaris])

HSP 1 Score: 340.5 bits (872), Expect = 3.4e-90
Identity = 181/319 (56.74%), Postives = 227/319 (71.16%), Query Frame = 1 

Query: 17  SISAIFTFFSVLILCSFK-----PISSNPQTH-----QTARSLCNGTLFPNLCFSKLSSF 76
           ++++I T  S L+  S         SS    H     Q A+S C GTL+P+LC S L++F
Sbjct: 5   TLASILTVISALLFLSTHFTNTPTRSSEKHLHFHKHMQVAQSTCQGTLYPDLCVSTLATF 64

Query: 77  PQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLSPTDRRALDDCLELLDGSI 136
           P L S S  ++  S +N T  EV  ++ NCS LK      L+P D+RALDDCL+L   + 
Sbjct: 65  PDLTSKSVPQVISSVVNHTMYEVKSSFYNCSSLKKKIRN-LNPLDQRALDDCLKLFQDTN 124

Query: 137 AELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVELYLGKIE 196
            EL+T+I DLS S   ++  HDL TL+S AMTN  TCLDGF  S G VR R+E  L +I 
Sbjct: 125 LELKTTIDDLSKSSIGSKRHHDLQTLLSGAMTNLYTCLDGFAYSKGRVRDRIEKKLLEIS 184

Query: 197 QHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKFNLV 256
            H+S  LAMLKK+PGVK   +  EV PEYG ++ GFP+WVS KDR+LLQA+VNE+KF+LV
Sbjct: 185 HHMSNSLAMLKKVPGVKTRTSKSEVFPEYGKMKKGFPLWVSPKDRKLLQASVNESKFDLV 244

Query: 257 VAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLVFVGDGIGKTL 316
           VAKDG+GNFTT+GEA+A APNSSTTRFVI+IK GAYFENVE+ RKKTNL+FVGDGIGKT+
Sbjct: 245 VAKDGTGNFTTIGEALALAPNSSTTRFVIHIKEGAYFENVEVIRKKTNLMFVGDGIGKTV 304

Query: 317 IKANRNVVDGWTTFRSATV 326
           +K +RNVVDGWTTF+SATV
Sbjct: 305 VKGSRNVVDGWTTFQSATV 322

[Close]
BLAST of Cp4.1LG12g05860 vs. NCBI nr
Match: gi|951019192|ref|XP_014512061.1| (PREDICTED: pectinesterase-like [Vigna radiata var. radiata])

HSP 1 Score: 336.7 bits (862), Expect = 4.9e-89
Identity = 179/319 (56.11%), Postives = 227/319 (71.16%), Query Frame = 1 

Query: 18  ISAIFTFFSVLILCSFKPISSNP----------QTH-QTARSLCNGTLFPNLCFSKLSSF 77
           +++IFT  S L+  +    ++ P          Q H Q A+S C GTL+P LC S L++F
Sbjct: 6   LASIFTVISALLFLATH-FTNTPTRATQKHLHFQNHIQVAQSTCQGTLYPELCVSTLATF 65

Query: 78  PQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLSPTDRRALDDCLELLDGSI 137
           P L S S  ++  S +N T  EV  ++ NCS LK      L+P D+RALDDCL+L   + 
Sbjct: 66  PDLTSKSVPQVISSVVNHTMYEVKSSFYNCSSLKKKIRN-LNPLDQRALDDCLKLFQDTN 125

Query: 138 AELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVELYLGKIE 197
            EL+T+I DLS S   ++  HDL TL+S AMTN  TCLDGF  S G VR R+E  L +I 
Sbjct: 126 LELKTTIDDLSTSPIGSKRRHDLQTLLSGAMTNLYTCLDGFAYSKGRVRDRIEKKLLEIS 185

Query: 198 QHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKFNLV 257
            H+S  LAMLKK+P VK+  +  E+ PEYG ++ GFP WVS  DR+LLQA+VNETKF+LV
Sbjct: 186 HHMSNSLAMLKKVPEVKKTTSKSELFPEYGKMKKGFPSWVSPNDRKLLQASVNETKFDLV 245

Query: 258 VAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLVFVGDGIGKTL 317
           VAKDG+GNFTT+GEA+A APNSSTTRFVI+IKAGAYFENVE+ RK+TNL+FVGDGIGKT+
Sbjct: 246 VAKDGTGNFTTIGEALAVAPNSSTTRFVIHIKAGAYFENVEVIRKQTNLMFVGDGIGKTV 305

Query: 318 IKANRNVVDGWTTFRSATV 326
           +K +RNVVDGWTTF+SATV
Sbjct: 306 VKGSRNVVDGWTTFQSATV 322

The following BLAST results are available for this feature:
BLAST of Cp4.1LG12g05860 vs. Swiss-Prot
Match NameE-valueIdentityDescription
PME40_ARATH5.8e-5849.81Probable pectinesterase/pectinesterase inhibitor 40 OS=Arabidopsis thaliana GN=P... [more]
PME2_ARATH1.2e-5042.30Pectinesterase 2 OS=Arabidopsis thaliana GN=PME2 PE=2 SV=2[more]
PME39_ARATH2.6e-5045.45Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana GN=P... [more]
PME3_CITSI1.0e-4940.67Pectinesterase 3 OS=Citrus sinensis PE=1 SV=1[more]
PME1_CITSI8.5e-4940.80Pectinesterase 1 OS=Citrus sinensis GN=PECS-1.1 PE=2 SV=1[more]
BLAST of Cp4.1LG12g05860 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0K5K8_CUCSA2.3e-13378.12Pectinesterase OS=Cucumis sativus GN=Csa_7G405320 PE=4 SV=1[more]
V7BH80_PHAVU2.4e-9056.74Pectinesterase OS=Phaseolus vulgaris GN=PHAVU_007G218500g PE=4 SV=1[more]
A0A0L9VH61_PHAAN3.8e-8860.28Pectinesterase OS=Phaseolus angularis GN=LR48_Vigan10g027800 PE=4 SV=1[more]
A0A0S3SSE1_PHAAN3.8e-8860.28Pectinesterase OS=Vigna angularis var. angularis GN=Vigan.08G252900 PE=4 SV=1[more]
K7K5X8_SOYBN3.2e-8760.14Pectinesterase OS=Glycine max GN=GLYMA_02G016500 PE=4 SV=1[more]
BLAST of Cp4.1LG12g05860 vs. TAIR10
Match NameE-valueIdentityDescription
AT4G02320.13.3e-5949.81 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT1G53830.16.6e-5242.30 pectin methylesterase 2[more]
AT4G02300.11.5e-5145.45 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT3G14310.12.2e-4737.97 pectin methylesterase 3[more]
AT5G53370.17.6e-4035.49 pectin methylesterase PCR fragment F[more]
BLAST of Cp4.1LG12g05860 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|659101319|ref|XP_008451545.1|1.2e-15186.77PREDICTED: pectinesterase-like [Cucumis melo][more]
gi|449436467|ref|XP_004136014.1|9.4e-14983.84PREDICTED: pectinesterase-like [Cucumis sativus][more]
gi|700189742|gb|KGN44975.1|3.2e-13378.12Pectinesterase [Cucumis sativus][more]
gi|593689158|ref|XP_007145198.1|3.4e-9056.74hypothetical protein PHAVU_007G218500g [Phaseolus vulgaris][more]
gi|951019192|ref|XP_014512061.1|4.9e-8956.11PREDICTED: pectinesterase-like [Vigna radiata var. radiata][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0004857enzyme inhibitor activity
GO:0030599pectinesterase activity
Vocabulary: Biological Process
TermDefinition
GO:0042545cell wall modification
Vocabulary: Cellular Component
TermDefinition
GO:0005618cell wall
Vocabulary: INTERPRO
TermDefinition
IPR012334Pectin_lyas_fold
IPR011050Pectin_lyase_fold/virulence
IPR006501Pectinesterase_inhib_dom
IPR000070Pectinesterase_cat
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
biological_process GO:0005982 starch metabolic process
biological_process GO:0005985 sucrose metabolic process
cellular_component GO:0005618 cell wall
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0004857 enzyme inhibitor activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG12g05860.1Cp4.1LG12g05860.1mRNA


InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 244..327
score: 2.9
IPR006501Pectinesterase inhibitor domainGENE3DG3DSA:1.20.140.40coord: 46..200
score: 1.2
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 47..194
score: 3.4
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 41..195
score: 6.3
IPR006501Pectinesterase inhibitor domainTIGRFAMsTIGR01614TIGR01614coord: 20..198
score: 3.7
IPR006501Pectinesterase inhibitor domainunknownSSF101148Plant invertase/pectin methylesterase inhibitorcoord: 47..200
score: 6.67
IPR011050Pectin lyase fold/virulence factorunknownSSF51126Pectin lyase-likecoord: 242..326
score: 2.35
IPR012334Pectin lyase foldGENE3DG3DSA:2.160.20.10coord: 242..327
score: 8.8
NoneNo IPR availablePANTHERPTHR31707FAMILY NOT NAMEDcoord: 11..326
score: 4.5E
NoneNo IPR availablePANTHERPTHR31707:SF25PECTINESTERASE/PECTINESTERASE INHIBITOR 39-RELATEDcoord: 11..326
score: 4.5E

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Cp4.1LG12g05860Cucsa.205350Cucumber (Gy14) v1cgycpeB0579
Cp4.1LG12g05860CmaCh17G010670Cucurbita maxima (Rimu)cmacpeB371
Cp4.1LG12g05860CmoCh17G010390Cucurbita moschata (Rifu)cmocpeB335
Cp4.1LG12g05860Cla015102Watermelon (97103) v1cpewmB131
Cp4.1LG12g05860Csa7G405320Cucumber (Chinese Long) v2cpecuB161
Cp4.1LG12g05860MELO3C015963Melon (DHL92) v3.5.1cpemeB118
Cp4.1LG12g05860ClCG09G005530Watermelon (Charleston Gray)cpewcgB126
Cp4.1LG12g05860CSPI07G17790Wild cucumber (PI 183967)cpecpiB163
Cp4.1LG12g05860Lsi02G024340Bottle gourd (USVL1VR-Ls)cpelsiB113
Cp4.1LG12g05860MELO3C015963.2Melon (DHL92) v3.6.1cpemedB145
Cp4.1LG12g05860CsaV3_7G029580Cucumber (Chinese Long) v3cpecucB0188
Cp4.1LG12g05860Bhi09G000758Wax gourdcpewgoB0210
Cp4.1LG12g05860CsGy7G016240Cucumber (Gy14) v2cgybcpeB906
Cp4.1LG12g05860Carg07156Silver-seed gourdcarcpeB0158
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Cp4.1LG12g05860Wax gourdcpewgoB0171
Cp4.1LG12g05860Cucurbita pepo (Zucchini)cpecpeB157
Cp4.1LG12g05860Cucumber (Gy14) v1cgycpeB0185
Cp4.1LG12g05860Cucurbita maxima (Rimu)cmacpeB260
Cp4.1LG12g05860Cucurbita maxima (Rimu)cmacpeB465
Cp4.1LG12g05860Cucurbita maxima (Rimu)cmacpeB902
Cp4.1LG12g05860Cucurbita moschata (Rifu)cmocpeB224
Cp4.1LG12g05860Cucurbita moschata (Rifu)cmocpeB425
Cp4.1LG12g05860Cucurbita moschata (Rifu)cmocpeB839
Cp4.1LG12g05860Wild cucumber (PI 183967)cpecpiB141
Cp4.1LG12g05860Cucumber (Chinese Long) v2cpecuB139
Cp4.1LG12g05860Bottle gourd (USVL1VR-Ls)cpelsiB116
Cp4.1LG12g05860Watermelon (Charleston Gray)cpewcgB134
Cp4.1LG12g05860Watermelon (97103) v1cpewmB141
Cp4.1LG12g05860Melon (DHL92) v3.5.1cpemeB146
Cp4.1LG12g05860Melon (DHL92) v3.6.1cpemedB172
Cp4.1LG12g05860Silver-seed gourdcarcpeB0801
Cp4.1LG12g05860Silver-seed gourdcarcpeB1070
Cp4.1LG12g05860Silver-seed gourdcarcpeB1143
Cp4.1LG12g05860Cucumber (Chinese Long) v3cpecucB0162