Bhi09G000758 (gene) Wax gourd

BLAST of Bhi09G000758 vs. Swiss-Prot
Match: sp|O81301|PME40_ARATH (Probable pectinesterase/pectinesterase inhibitor 40 OS=Arabidopsis thaliana OX=3702 GN=PME40 PE=2 SV=1)

HSP 1 Score: 542.7 bits (1397), Expect = 4.6e-153
Identity = 278/488 (56.97%), Postives = 351/488 (71.93%), Query Frame = 0

Query: 75  KLAGSAINFTTREVLGAYANCTKLKTILYGGLNPTDRRALDDCLELLDSSIAELQASICD 134
           +L  +A+N T   V  + +N + L   L   L+  D  A DDCLELLD ++ +L  +I  
Sbjct: 39  ELVVTALNQTISNVNLSSSNFSDLLQRLGSNLSHRDLCAFDDCLELLDDTVFDLTTAISK 98

Query: 135 LAPPQSAARHSHDLLTLISAAMTNHRTCVDGFYNSSGT------------VRSRVELYLG 194
           L   +S +   H++  L+SAAMTN RTC+DGF +S               V   ++  L 
Sbjct: 99  L---RSHSPELHNVKMLLSAAMTNTRTCLDGFASSDNDENLNNNDNKTYGVAESLKESLF 158

Query: 195 KIGQHLSIDLAMLKKIPGVNRAPAAGEVLPEYGAVKGGFPNWVSVKDRRLLQAAVNETKF 254
            I  H+S  LAML+ IPG       G+V  +      GFP WVS  DR LLQ  V+ETK 
Sbjct: 159 NISSHVSDSLAMLENIPG----HIPGKVKEDV-----GFPMWVSGSDRNLLQDPVDETKV 218

Query: 255 NLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERRKTNLMFVGDGIG 314
           NLVVA++G+GN+TT+GEA++AAPNSS TRFVIYIK G YFEN+EI R KT +MF+GDGIG
Sbjct: 219 NLVVAQNGTGNYTTIGEAISAAPNSSETRFVIYIKCGEYFENIEIPREKTMIMFIGDGIG 278

Query: 315 KTLIKADRNVVDGWTTFRSATVAVVGNGFIAKGITFENYAGPSKHQAVALRSNSDFSAFY 374
           +T+IKA+R+  DGWT F SATV V G+GFIAK ++F NYAGP KHQAVALRS+SD SA+Y
Sbjct: 279 RTVIKANRSYADGWTAFHSATVGVRGSGFIAKDLSFVNYAGPEKHQAVALRSSSDLSAYY 338

Query: 375 QCSFVGYQDTLYVHSLRQFYRDCDIYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTA 434
           +CSF  YQDT+YVHS +QFYR+CDIYGT+DFIFG+A+VVFQNC+LYAR+PN NQ+ I+TA
Sbjct: 339 RCSFESYQDTIYVHSHKQFYRECDIYGTVDFIFGDASVVFQNCSLYARRPNPNQKIIYTA 398

Query: 435 QGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRTVFLRSFIGQLVDPA 494
           QGRE+  + TGISI++ ++ AA DLIPV ++F+ YLGRPW+LYSRTV ++SFI  LVDPA
Sbjct: 399 QGRENSREPTGISIISSRILAAPDLIPVQANFKAYLGRPWQLYSRTVIMKSFIDDLVDPA 458

Query: 495 GWLEWNGTFALDTLYYGEYMNRGPGSNTTGRVTWPGYRVITNASEASQFTVEGFIQGSSW 551
           GWL+W   FAL+TLYYGEYMN GPGSN T RV WPG++ I    EASQF+V  FI G+ W
Sbjct: 459 GWLKWKDDFALETLYYGEYMNEGPGSNMTNRVQWPGFKRIETVEEASQFSVGPFIDGNKW 514

BLAST of Bhi09G000758 vs. Swiss-Prot
Match: sp|P85076|PME_ACTDE (Pectinesterase OS=Actinidia deliciosa OX=3627 PE=1 SV=1)

HSP 1 Score: 514.6 bits (1324), Expect = 1.4e-144
Identity = 248/319 (77.74%), Postives = 275/319 (86.21%), Query Frame = 0

Query: 236 AVNETKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERRKTNLM 295
           AV +   ++VVAKDGSGNFTTVG AVAAA +SST RFVIYIK GAYFE V+++++KTNLM
Sbjct: 2   AVTDIVPDVVVAKDGSGNFTTVGAAVAAAKDSSTARFVIYIKEGAYFEYVDVDKKKTNLM 61

Query: 296 FVGDGIGKTLIKADRNVVDGWTTFRSATVAVVGNGFIAKGITFENYAGPSKHQAVALRSN 355
           F+GDGIGKT IK +R+VVDGWTTFRS+TVAVVG GFIA+GI+FENYAGPSKHQAVALRS 
Sbjct: 62  FIGDGIGKTWIKGNRSVVDGWTTFRSSTVAVVGTGFIARGISFENYAGPSKHQAVALRSG 121

Query: 356 SDFSAFYQCSFVGYQDTLYVHSLRQFYRDCDIYGTIDFIFGNAAVVFQNCNLYARKPNSN 415
           +DFSAFYQCSFVGYQDTLYVHSLRQFY +CD+YGTIDFIFGNAA V Q CNLYARKPN N
Sbjct: 122 ADFSAFYQCSFVGYQDTLYVHSLRQFYSECDVYGTIDFIFGNAAAVLQKCNLYARKPNEN 181

Query: 416 QRNIFTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRTVFLRSFI 475
           Q+NIFTAQGR+DPNQNTGISILNCKV AA+DLIPVLSSF+TYLGRPWK YSRTVFL S +
Sbjct: 182 QKNIFTAQGRDDPNQNTGISILNCKVAAAADLIPVLSSFKTYLGRPWKEYSRTVFLLSQM 241

Query: 476 GQLVDPAGWLEWNGTFALDTLYYGEYMNRGPGSNTTGRVTWPGYRVITNASEASQFTVEG 535
             L+DPAGWLEW+G FAL TLYY EY N GPGSNTT RVTWPGY V TN +E  QFTV  
Sbjct: 242 ESLIDPAGWLEWSGDFALTTLYYREYKNTGPGSNTTARVTWPGYAVTTNETEVIQFTVGN 301

Query: 536 FIQGSSWLNSTGIPFFSGL 555
           FIQGS WL S  IP +  L
Sbjct: 302 FIQGSQWLTSYNIPVYLNL 320

BLAST of Bhi09G000758 vs. Swiss-Prot
Match: sp|O81415|PME39_ARATH (Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana OX=3702 GN=PME39 PE=2 SV=1)

HSP 1 Score: 510.0 bits (1312), Expect = 3.3e-143
Identity = 264/481 (54.89%), Postives = 335/481 (69.65%), Query Frame = 0

Query: 81  INFTTREVLGAYANCTKLKTILYGGLNPTDRRALDDCLELLDSSIAELQASICDLAPPQS 140
           +N T  +V  A +N + L+T L+  L   +R A +DCL LLD +I++L+         +S
Sbjct: 61  LNLTILKVNLASSNFSDLQTRLFPNLTHYERCAFEDCLGLLDDTISDLE---XXXXXXRS 120

Query: 141 AARHSHDLLTLISAAMTNHRTCVDGFYNSSGTVRSRVELYLGK--------IGQHLSIDL 200
           ++   +D+  L++  MT   TC+DGF  S     + +   L +        I  +LS  L
Sbjct: 121 SSLEFNDISMLLTNVMTYQDTCLDGFSTSDNENNNDMTYELPENLKEIILDISNNLSNSL 180

Query: 201 AMLKKIPGVNRAPAAGEVLPEYGAVKGGFPNWVSVKDRRLLQAAVNETKFNLVVAKDGSG 260
            ML+ I     +P + EV  EY       P+W+S  D+RLL+A V ET +NL VA DG+G
Sbjct: 181 HMLQVISRKKPSPKSSEVDVEY-------PSWLSENDQRLLEAPVQETNYNLSVAIDGTG 240

Query: 261 NFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERRKTNLMFVGDGIGKTLIKADRNV 320
           NFTT+ +AV AAPN S TRF+IYIK G YFENVE+ ++KT +MF+GDGIGKT+IKA+R+ 
Sbjct: 241 NFTTINDAVFAAPNMSETRFIIYIKGGEYFENVELPKKKTMIMFIGDGIGKTVIKANRSR 300

Query: 321 VDGWTTFRSATVAVVGNGFIAKGITFENYAGPSKHQAVALRSNSDFSAFYQCSFVGYQDT 380
           +DGW+TF++ TV V G G+IAK I+F N AGP+K QAVA RS SD SAFY+C F GYQDT
Sbjct: 301 IDGWSTFQTPTVGVKGKGYIAKDISFVNSAGPAKAQAVAFRSGSDHSAFYRCEFDGYQDT 360

Query: 381 LYVHSLRQFYRDCDIYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGREDPNQNT 440
           LYVHS +QFYR+CDIYGTIDFIFGNAAVVFQN +LYARKPN   +  FTAQ R   +Q T
Sbjct: 361 LYVHSAKQFYRECDIYGTIDFIFGNAAVVFQNSSLYARKPNPGHKIAFTAQSRNQSDQPT 420

Query: 441 GISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRTVFLRSFIGQLVDPAGWLEWNGTFA 500
           GISILNC++ AA DLIPV  +F+ YLGRPW+ YSRTV ++SFI  L+ PAGWLE    FA
Sbjct: 421 GISILNCRILAAPDLIPVKENFKAYLGRPWRKYSRTVIIKSFIDDLIHPAGWLEGKKDFA 480

Query: 501 LDTLYYGEYMNRGPGSNTTGRVTWPGYRVITNASEASQFTVEGFIQGSSWLNSTGIPFFS 554
           L+TLYYGEYMN GPG+N   RVTWPG+R I N +EA+QFTV  FI GS+WLNSTGIPF  
Sbjct: 481 LETLYYGEYMNEGPGANMAKRVTWPGFRRIENQTEATQFTVGPFIDGSTWLNSTGIPFSL 531

BLAST of Bhi09G000758 vs. Swiss-Prot
Match: sp|O04886|PME1_CITSI (Pectinesterase 1 OS=Citrus sinensis OX=2711 GN=PECS-1.1 PE=2 SV=1)

HSP 1 Score: 497.3 bits (1279), Expect = 2.2e-139
Identity = 268/527 (50.85%), Postives = 344/527 (65.28%), Query Frame = 0

Query: 38  NPQIHQTARSLCNGTLFPHLCFSKLSSFPQLAS--LSPEKLAGSAINFTTREVLGAYANC 97
           N   H   +S C+ T +P LCFS +++ P+ +    S + +   ++N TT  V   Y   
Sbjct: 62  NEPHHAILKSSCSSTRYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGI 121

Query: 98  TKLKTILYGGLNPTDRRALDDCLELLDSSIAELQASICDLAP---PQSAARHSHDLLTLI 157
            KL  +    L   ++ AL DCLE +D ++ EL  ++ DL      +S ++H+ DL TL+
Sbjct: 122 QKL--LKRTNLTKREKVALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLM 181

Query: 158 SAAMTNHRTCVDGFYNSSGTVRSRVELYLGK--IGQHLSIDLAMLKKIPGVN---RAPAA 217
           SAAMTN  TC+DGF +       R  L  G+  + +  S  LAM+K +   +      + 
Sbjct: 182 SAAMTNQGTCLDGFSHDDANKHVRDALSDGQVHVEKMCSNALAMIKNMTDTDMMIMRTSN 241

Query: 218 GEVLPEYGAVKGGFPNWVSVKDRRLLQAAVNETKFNLVVAKDGSGNFTTVGEAVAAAPNS 277
              L E  +   G+P W+S  DRRLLQ+  +    N VVA DGSGNF TV  AVAAAP  
Sbjct: 242 NRKLTEETSTVDGWPAWLSPGDRRLLQS--SSVTPNAVVAADGSGNFKTVAAAVAAAPQG 301

Query: 278 STTRFVIYIKAGAYFENVEIERRKTNLMFVGDGIGKTLIKADRNVVDGWTTFRSATVAVV 337
            T R++I IKAG Y ENVE+ ++  N+MF+GDG  +T+I   RNVVDG TTF+SAT AVV
Sbjct: 302 GTKRYIIRIKAGVYRENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATAAVV 361

Query: 338 GNGFIAKGITFENYAGPSKHQAVALRSNSDFSAFYQCSFVGYQDTLYVHSLRQFYRDCDI 397
           G GF+A+ ITF+N AGPSKHQAVALR  +D SAFY C  + YQDTLYVHS RQF+ +C I
Sbjct: 362 GEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCLI 421

Query: 398 YGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGREDPNQNTGISILNCKVEAASDL 457
            GT+DFIFGNAA V QNC+++ARKPNS Q+N+ TAQGR DPNQNTGI I   ++ A SDL
Sbjct: 422 AGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGATSDL 481

Query: 458 IPVLSSFRTYLGRPWKLYSRTVFLRSFIGQLVDPAGWLEWNGTFALDTLYYGEYMNRGPG 517
            PV  SF TYLGRPWK YSRTV ++S I  L+ PAGW EW+G FAL+TL+YGE+ N G G
Sbjct: 482 KPVQGSFPTYLGRPWKEYSRTVIMQSSITDLIHPAGWHEWDGNFALNTLFYGEHQNSGAG 541

Query: 518 SNTTGRVTWPGYRVITNASEASQFTVEGFIQGSSWLNSTGIPFFSGL 555
           + T+GRV W G+RVIT+A+EA  FT   FI GSSWL STG PF  GL
Sbjct: 542 AGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584

BLAST of Bhi09G000758 vs. Swiss-Prot
Match: sp|P83948|PME3_CITSI (Pectinesterase 3 OS=Citrus sinensis OX=2711 PE=1 SV=1)

HSP 1 Score: 497.3 bits (1279), Expect = 2.2e-139
Identity = 267/527 (50.66%), Postives = 346/527 (65.65%), Query Frame = 0

Query: 38  NPQIHQTARSLCNGTLFPHLCFSKLSSFPQLAS--LSPEKLAGSAINFTTREVLGAYANC 97
           N   H   +S C+ T +P LCFS +++ P+ +    S + +   ++N TT  V   Y   
Sbjct: 62  NEPHHAILKSSCSSTRYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGI 121

Query: 98  TKLKTILYGGLNPTDRRALDDCLELLDSSIAELQASICDLAP---PQSAARHSHDLLTLI 157
            KL  +    L   ++ AL DCLE +D ++ EL  ++ DL      +S ++H+ DL TL+
Sbjct: 122 QKL--LKRTNLTKREKVALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLM 181

Query: 158 SAAMTNHRTCVDGFYNSSGTVRSRVELYLGK--IGQHLSIDLAMLKKIPGVN---RAPAA 217
           SAAMTN  TC+DGF +       R  L  G+  + +  S  LAM+K +   +      + 
Sbjct: 182 SAAMTNQGTCLDGFSHDDANKHVRDALSDGQVHVEKMCSNALAMIKNMTDTDMMIMRTSN 241

Query: 218 GEVLPEYGAVKGGFPNWVSVKDRRLLQAAVNETKFNLVVAKDGSGNFTTVGEAVAAAPNS 277
              L E  +   G+P W+S  DRRLLQ+  +    N+VVA DGSGNF TV  +VAAAP  
Sbjct: 242 NRKLIEETSTVDGWPAWLSTGDRRLLQS--SSVTPNVVVAADGSGNFKTVAASVAAAPQG 301

Query: 278 STTRFVIYIKAGAYFENVEIERRKTNLMFVGDGIGKTLIKADRNVVDGWTTFRSATVAVV 337
            T R++I IKAG Y ENVE+ ++  N+MF+GDG  +T+I   RNVVDG TTF+SATVAVV
Sbjct: 302 GTKRYIIRIKAGVYRENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATVAVV 361

Query: 338 GNGFIAKGITFENYAGPSKHQAVALRSNSDFSAFYQCSFVGYQDTLYVHSLRQFYRDCDI 397
           G GF+A+ ITF+N AGPSKHQAVALR  +D SAFY C  + YQDTLYVHS RQF+ +C I
Sbjct: 362 GEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCLI 421

Query: 398 YGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGREDPNQNTGISILNCKVEAASDL 457
            GT+DFIFGNAA V QNC+++ARKPNS Q+N+ TAQGR DPNQNTGI I   ++ A SDL
Sbjct: 422 AGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRADPNQNTGIVIQKSRIGATSDL 481

Query: 458 IPVLSSFRTYLGRPWKLYSRTVFLRSFIGQLVDPAGWLEWNGTFALDTLYYGEYMNRGPG 517
            PV  SF TYLGRPWK YSRTV ++S I  ++ PAGW EW+G FAL+TL+YGE+ N G G
Sbjct: 482 KPVQGSFPTYLGRPWKEYSRTVIMQSSITDVIHPAGWHEWDGNFALNTLFYGEHQNAGAG 541

Query: 518 SNTTGRVTWPGYRVITNASEASQFTVEGFIQGSSWLNSTGIPFFSGL 555
           + T+GRV W G+RVIT+A+EA  FT   FI GSSWL STG PF  GL
Sbjct: 542 AGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584

BLAST of Bhi09G000758 vs. TAIR10
Match: AT4G02320.1 (Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 542.7 bits (1397), Expect = 2.6e-154
Identity = 278/488 (56.97%), Postives = 351/488 (71.93%), Query Frame = 0

Query: 75  KLAGSAINFTTREVLGAYANCTKLKTILYGGLNPTDRRALDDCLELLDSSIAELQASICD 134
           +L  +A+N T   V  + +N + L   L   L+  D  A DDCLELLD ++ +L  +I  
Sbjct: 39  ELVVTALNQTISNVNLSSSNFSDLLQRLGSNLSHRDLCAFDDCLELLDDTVFDLTTAISK 98

Query: 135 LAPPQSAARHSHDLLTLISAAMTNHRTCVDGFYNSSGT------------VRSRVELYLG 194
           L   +S +   H++  L+SAAMTN RTC+DGF +S               V   ++  L 
Sbjct: 99  L---RSHSPELHNVKMLLSAAMTNTRTCLDGFASSDNDENLNNNDNKTYGVAESLKESLF 158

Query: 195 KIGQHLSIDLAMLKKIPGVNRAPAAGEVLPEYGAVKGGFPNWVSVKDRRLLQAAVNETKF 254
            I  H+S  LAML+ IPG       G+V  +      GFP WVS  DR LLQ  V+ETK 
Sbjct: 159 NISSHVSDSLAMLENIPG----HIPGKVKEDV-----GFPMWVSGSDRNLLQDPVDETKV 218

Query: 255 NLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERRKTNLMFVGDGIG 314
           NLVVA++G+GN+TT+GEA++AAPNSS TRFVIYIK G YFEN+EI R KT +MF+GDGIG
Sbjct: 219 NLVVAQNGTGNYTTIGEAISAAPNSSETRFVIYIKCGEYFENIEIPREKTMIMFIGDGIG 278

Query: 315 KTLIKADRNVVDGWTTFRSATVAVVGNGFIAKGITFENYAGPSKHQAVALRSNSDFSAFY 374
           +T+IKA+R+  DGWT F SATV V G+GFIAK ++F NYAGP KHQAVALRS+SD SA+Y
Sbjct: 279 RTVIKANRSYADGWTAFHSATVGVRGSGFIAKDLSFVNYAGPEKHQAVALRSSSDLSAYY 338

Query: 375 QCSFVGYQDTLYVHSLRQFYRDCDIYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTA 434
           +CSF  YQDT+YVHS +QFYR+CDIYGT+DFIFG+A+VVFQNC+LYAR+PN NQ+ I+TA
Sbjct: 339 RCSFESYQDTIYVHSHKQFYRECDIYGTVDFIFGDASVVFQNCSLYARRPNPNQKIIYTA 398

Query: 435 QGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRTVFLRSFIGQLVDPA 494
           QGRE+  + TGISI++ ++ AA DLIPV ++F+ YLGRPW+LYSRTV ++SFI  LVDPA
Sbjct: 399 QGRENSREPTGISIISSRILAAPDLIPVQANFKAYLGRPWQLYSRTVIMKSFIDDLVDPA 458

Query: 495 GWLEWNGTFALDTLYYGEYMNRGPGSNTTGRVTWPGYRVITNASEASQFTVEGFIQGSSW 551
           GWL+W   FAL+TLYYGEYMN GPGSN T RV WPG++ I    EASQF+V  FI G+ W
Sbjct: 459 GWLKWKDDFALETLYYGEYMNEGPGSNMTNRVQWPGFKRIETVEEASQFSVGPFIDGNKW 514

BLAST of Bhi09G000758 vs. TAIR10
Match: AT4G02300.1 (Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 510.0 bits (1312), Expect = 1.9e-144
Identity = 264/481 (54.89%), Postives = 335/481 (69.65%), Query Frame = 0

Query: 81  INFTTREVLGAYANCTKLKTILYGGLNPTDRRALDDCLELLDSSIAELQASICDLAPPQS 140
           +N T  +V  A +N + L+T L+  L   +R A +DCL LLD +I++L+         +S
Sbjct: 61  LNLTILKVNLASSNFSDLQTRLFPNLTHYERCAFEDCLGLLDDTISDLE---XXXXXXRS 120

Query: 141 AARHSHDLLTLISAAMTNHRTCVDGFYNSSGTVRSRVELYLGK--------IGQHLSIDL 200
           ++   +D+  L++  MT   TC+DGF  S     + +   L +        I  +LS  L
Sbjct: 121 SSLEFNDISMLLTNVMTYQDTCLDGFSTSDNENNNDMTYELPENLKEIILDISNNLSNSL 180

Query: 201 AMLKKIPGVNRAPAAGEVLPEYGAVKGGFPNWVSVKDRRLLQAAVNETKFNLVVAKDGSG 260
            ML+ I     +P + EV  EY       P+W+S  D+RLL+A V ET +NL VA DG+G
Sbjct: 181 HMLQVISRKKPSPKSSEVDVEY-------PSWLSENDQRLLEAPVQETNYNLSVAIDGTG 240

Query: 261 NFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERRKTNLMFVGDGIGKTLIKADRNV 320
           NFTT+ +AV AAPN S TRF+IYIK G YFENVE+ ++KT +MF+GDGIGKT+IKA+R+ 
Sbjct: 241 NFTTINDAVFAAPNMSETRFIIYIKGGEYFENVELPKKKTMIMFIGDGIGKTVIKANRSR 300

Query: 321 VDGWTTFRSATVAVVGNGFIAKGITFENYAGPSKHQAVALRSNSDFSAFYQCSFVGYQDT 380
           +DGW+TF++ TV V G G+IAK I+F N AGP+K QAVA RS SD SAFY+C F GYQDT
Sbjct: 301 IDGWSTFQTPTVGVKGKGYIAKDISFVNSAGPAKAQAVAFRSGSDHSAFYRCEFDGYQDT 360

Query: 381 LYVHSLRQFYRDCDIYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGREDPNQNT 440
           LYVHS +QFYR+CDIYGTIDFIFGNAAVVFQN +LYARKPN   +  FTAQ R   +Q T
Sbjct: 361 LYVHSAKQFYRECDIYGTIDFIFGNAAVVFQNSSLYARKPNPGHKIAFTAQSRNQSDQPT 420

Query: 441 GISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRTVFLRSFIGQLVDPAGWLEWNGTFA 500
           GISILNC++ AA DLIPV  +F+ YLGRPW+ YSRTV ++SFI  L+ PAGWLE    FA
Sbjct: 421 GISILNCRILAAPDLIPVKENFKAYLGRPWRKYSRTVIIKSFIDDLIHPAGWLEGKKDFA 480

Query: 501 LDTLYYGEYMNRGPGSNTTGRVTWPGYRVITNASEASQFTVEGFIQGSSWLNSTGIPFFS 554
           L+TLYYGEYMN GPG+N   RVTWPG+R I N +EA+QFTV  FI GS+WLNSTGIPF  
Sbjct: 481 LETLYYGEYMNEGPGANMAKRVTWPGFRRIENQTEATQFTVGPFIDGSTWLNSTGIPFSL 531

BLAST of Bhi09G000758 vs. TAIR10
Match: AT1G53830.1 (pectin methylesterase 2)

HSP 1 Score: 496.5 bits (1277), Expect = 2.1e-140
Identity = 271/530 (51.13%), Postives = 343/530 (64.72%), Query Frame = 0

Query: 42  HQTARSLCNGTLFPHLCFSKLSSFPQLASLSPEKLAGSAINFTTREVLGAYANCTKLKTI 101
           H   +S+C+ TL+P LCFS +++       S +++  +++N TT+ V   Y    KL   
Sbjct: 59  HAILKSVCSSTLYPELCFSAVAATGGKELTSQKEVIEASLNLTTKAVKHNYFAVKKL-IA 118

Query: 102 LYGGLNPTDRRALDDCLELLDSSIAELQASICDL--APPQSAAR-HSHDLLTLISAAMTN 161
              GL P +  AL DCLE +D ++ EL  ++ DL   P Q + R H+ DL TLIS+A+TN
Sbjct: 119 KRKGLTPREVTALHDCLETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAITN 178

Query: 162 HRTCVDGFYNSSGTVRSRVELYLGKIG-QHL-SIDLAMLKKIPGV--------------- 221
             TC+DGF       + R  L  G++  +H+ S  LAM+K +                  
Sbjct: 179 QGTCLDGFSYDDADRKVRKALLKGQVHVEHMCSNALAMIKNMTETDIANFELRDKSSTFT 238

Query: 222 -NRAPAAGEVLPEYGAVKGGFPNWVSVKDRRLLQAAVNETKFNLVVAKDGSGNFTTVGEA 281
            N      EV  +  +   G+P W+SV DRRLLQ +    K +  VA DGSG+FTTV  A
Sbjct: 239 NNNNRKLKEVTGDLDS--DGWPKWLSVGDRRLLQGST--IKADATVADDGSGDFTTVAAA 298

Query: 282 VAAAPNSSTTRFVIYIKAGAYFENVEIERRKTNLMFVGDGIGKTLIKADRNVVDGWTTFR 341
           VAAAP  S  RFVI+IKAG Y ENVE+ ++KTN+MF+GDG GKT+I   RNVVDG TTF 
Sbjct: 299 VAAAPEKSNKRFVIHIKAGVYRENVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGSTTFH 358

Query: 342 SATVAVVGNGFIAKGITFENYAGPSKHQAVALRSNSDFSAFYQCSFVGYQDTLYVHSLRQ 401
           SATVA VG  F+A+ ITF+N AGPSKHQAVALR  SDFSAFYQC    YQDTLYVHS RQ
Sbjct: 359 SATVAAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLYVHSNRQ 418

Query: 402 FYRDCDIYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGREDPNQNTGISILNCK 461
           F+  C I GT+DFIFGNAA V Q+C++ AR+PNS Q+N+ TAQGR DPNQNTGI I NC+
Sbjct: 419 FFVKCHITGTVDFIFGNAAAVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVIQNCR 478

Query: 462 VEAASDLIPVLSSFRTYLGRPWKLYSRTVFLRSFIGQLVDPAGWLEWNGTFALDTLYYGE 521
           +   SDL+ V  +F TYLGRPWK YSRTV ++S I  ++ P GW EW+G+FALDTL Y E
Sbjct: 479 IGGTSDLLAVKGTFPTYLGRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTLTYRE 538

Query: 522 YMNRGPGSNTTGRVTWPGYRVITNASEASQFTVEGFIQGSSWLNSTGIPF 551
           Y+NRG G+ T  RV W GY+VIT+ +EA  FT   FI G  WL STG PF
Sbjct: 539 YLNRGGGAGTANRVKWKGYKVITSDTEAQPFTAGQFIGGGGWLASTGFPF 583

BLAST of Bhi09G000758 vs. TAIR10
Match: AT5G53370.1 (pectin methylesterase PCR fragment F)

HSP 1 Score: 484.2 bits (1245), Expect = 1.1e-136
Identity = 255/524 (48.66%), Postives = 332/524 (63.36%), Query Frame = 0

Query: 43  QTARSLCNGTLFPHLCFSKLSSFPQLASLSPEKLAGSAINFTTREVLGAYANCTKLKTIL 102
           Q     C+ +L+P+LC   L  FP   +    +L   + N T ++   A    +   TI 
Sbjct: 73  QAISRTCSKSLYPNLCIDTLLDFPGSLTADENELIHISFNATLQKFSKALYTSS---TIT 132

Query: 103 YGGLNPTDRRALDDCLELLDSSIAELQASICDLAPPQSAARHSHDLLTLISAAMTNHRTC 162
           Y  + P  R A D CLELLD S+  L  ++  +        HS D++T +S+AMTNH TC
Sbjct: 133 YTQMPPRVRSAYDSCLELLDDSVDALTRALSSVVVVSGDESHS-DVMTWLSSAMTNHDTC 192

Query: 163 VDGF---YNSSGTVRSRVELYLGKIGQHLSIDLAM-------LKKIPGVNRAPAAGEVLP 222
            DGF       G V+ +V   +  + + +S  LA+       L  +P VN     G    
Sbjct: 193 TDGFDEIEGQGGEVKDQVIGAVKDLSEMVSNCLAIFAGKVKDLSGVPVVNNRKLLGTEET 252

Query: 223 EYGAVKGGFPNWVSVKDRRLLQAAVNETKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRF 282
           E        PNW+  +DR LL    +  + ++ V+KDGSG F T+ EA+  AP  S+ RF
Sbjct: 253 E------ELPNWLKREDRELLGTPTSAIQADITVSKDGSGTFKTIAEAIKKAPEHSSRRF 312

Query: 283 VIYIKAGAY-FENVEIERRKTNLMFVGDGIGKTLIKADRNVVDGWTTFRSATVAVVGNGF 342
           VIY+KAG Y  EN+++ R+KTNLMF+GDG GKT+I   +++ D  TTF +AT A  G GF
Sbjct: 313 VIYVKAGRYEEENLKVGRKKTNLMFIGDGKGKTVITGGKSIADDLTTFHTATFAATGAGF 372

Query: 343 IAKGITFENYAGPSKHQAVALRSNSDFSAFYQCSFVGYQDTLYVHSLRQFYRDCDIYGTI 402
           I + +TFENYAGP+KHQAVALR   D +  Y+C+ +GYQD LYVHS RQF+R+C+IYGT+
Sbjct: 373 IVRDMTFENYAGPAKHQAVALRVGGDHAVVYRCNIIGYQDALYVHSNRQFFRECEIYGTV 432

Query: 403 DFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGREDPNQNTGISILNCKVEAASDLIPVL 462
           DFIFGNAAV+ Q+CN+YARKP + Q+   TAQ R+DPNQNTGISI  CK+ A  DL    
Sbjct: 433 DFIFGNAAVILQSCNIYARKPMAQQKITITAQNRKDPNQNTGISIHACKLLATPDLEASK 492

Query: 463 SSFRTYLGRPWKLYSRTVFLRSFIGQLVDPAGWLEWNGTFALDTLYYGEYMNRGPGSNTT 522
            S+ TYLGRPWKLYSR V++ S +G  +DP GWLEWNG FALD+LYYGEYMN+G GS   
Sbjct: 493 GSYPTYLGRPWKLYSRVVYMMSDMGDHIDPRGWLEWNGPFALDSLYYGEYMNKGLGSGIG 552

Query: 523 GRVTWPGYRVITNASEASQFTVEGFIQGSSWLNSTGIPFFSGLA 556
            RV WPGY VIT+  EAS+FTV  FI GSSWL STG+ FFSGL+
Sbjct: 553 QRVKWPGYHVITSTVEASKFTVAQFISGSSWLPSTGVSFFSGLS 586

BLAST of Bhi09G000758 vs. TAIR10
Match: AT3G49220.1 (Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 476.1 bits (1224), Expect = 3.0e-134
Identity = 253/525 (48.19%), Postives = 334/525 (63.62%), Query Frame = 0

Query: 43  QTARSLCNGTLFPHLCFSKLSSFP-QLASLSPEKLAGSAINFTTREVLGAYANCTKLKTI 102
           Q     C  T FP LC   L  FP  LA+ S + L    +N T      A  +   L  +
Sbjct: 85  QAISKACELTRFPELCVDSLMDFPGSLAASSSKDLIHVTVNMTLHHFSHALYSSASLSFV 144

Query: 103 LYGGLNPTDRRALDDCLELLDSSIAELQASICDLAPPQSAARHSHDLLTLISAAMTNHRT 162
               + P  R A D C+ELLD S+  L  ++  +    S++    D+ T +SAA+TNH T
Sbjct: 145 ---DMPPRARSAYDSCVELLDDSVDALSRALSSVV---SSSAKPQDVTTWLSAALTNHDT 204

Query: 163 CVDGFYN-SSGTVRSRVELYLGKIGQHLSIDLAMLKK---------IPGVNRAPAAGEVL 222
           C +GF     G V+  +   L  + + +S  LA+            +P  NR     E  
Sbjct: 205 CTEGFDGVDDGGVKDHMTAALQNLSELVSNCLAIFSASHDGDDFAGVPIQNRRLLGVEER 264

Query: 223 PEYGAVKGGFPNWVSVKDRRLLQAAVNETKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTR 282
            E       FP W+  K+R +L+  V++ + +++V+KDG+G   T+ EA+  AP +ST R
Sbjct: 265 EE------KFPRWMRPKEREILEMPVSQIQADIIVSKDGNGTCKTISEAIKKAPQNSTRR 324

Query: 283 FVIYIKAGAYFE-NVEIERRKTNLMFVGDGIGKTLIKADRNVVDGWTTFRSATVAVVGNG 342
            +IY+KAG Y E N+++ R+K NLMFVGDG GKT+I   +++ D  TTF +A+ A  G G
Sbjct: 325 IIIYVKAGRYEENNLKVGRKKINLMFVGDGKGKTVISGGKSIFDNITTFHTASFAATGAG 384

Query: 343 FIAKGITFENYAGPSKHQAVALRSNSDFSAFYQCSFVGYQDTLYVHSLRQFYRDCDIYGT 402
           FIA+ ITFEN+AGP+KHQAVALR  +D +  Y+C+ +GYQDTLYVHS RQF+R+CDIYGT
Sbjct: 385 FIARDITFENWAGPAKHQAVALRIGADHAVIYRCNIIGYQDTLYVHSNRQFFRECDIYGT 444

Query: 403 IDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGREDPNQNTGISILNCKVEAASDLIPV 462
           +DFIFGNAAVV QNC++YARKP   Q+N  TAQ R+DPNQNTGISI   +V AASDL   
Sbjct: 445 VDFIFGNAAVVLQNCSIYARKPMDFQKNTITAQNRKDPNQNTGISIHASRVLAASDLQAT 504

Query: 463 LSSFRTYLGRPWKLYSRTVFLRSFIGQLVDPAGWLEWNGTFALDTLYYGEYMNRGPGSNT 522
             S +TYLGRPWKL+SRTV++ S+IG  V   GWLEWN TFALDTLYYGEY+N GPGS  
Sbjct: 505 NGSTQTYLGRPWKLFSRTVYMMSYIGGHVHTRGWLEWNTTFALDTLYYGEYLNSGPGSGL 564

Query: 523 TGRVTWPGYRVITNASEASQFTVEGFIQGSSWLNSTGIPFFSGLA 556
             RV+WPGYRVI + +EA++FTV  FI GSSWL STG+ F +GL+
Sbjct: 565 GQRVSWPGYRVINSTAEANRFTVAEFIYGSSWLPSTGVSFLAGLS 597

BLAST of Bhi09G000758 vs. TrEMBL
Match: tr|A0A1S3BSU3|A0A1S3BSU3_CUCME (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103492792 PE=3 SV=1)

HSP 1 Score: 1011.5 bits (2614), Expect = 7.1e-292
Identity = 503/554 (90.79%), Postives = 515/554 (92.96%), Query Frame = 0

Query: 1   MYGLKNRAQIPSNPTXXXXXXXXXXXXXXXXXKPISSNPQIHQTARSLCNGTLFPHLCFS 60
           MYGL+NRAQI SNPT                 KPISSNPQIHQTARSLCNGTLFP+LCFS
Sbjct: 1   MYGLRNRAQISSNPTSISTISTFFFIFFLFSSKPISSNPQIHQTARSLCNGTLFPNLCFS 60

Query: 61  KLSSFPQLASLSPEKLAGSAINFTTREVLGAYANCTKLKTILYGGLNPTDRRALDDCLEL 120
           KLSSFPQLASLSPEKLAGSA+NFTTREVL AYANCT LKT LY GLNPTDR ALDDCLEL
Sbjct: 61  KLSSFPQLASLSPEKLAGSALNFTTREVLLAYANCTNLKTHLYCGLNPTDRHALDDCLEL 120

Query: 121 LDSSIAELQASICDLAPPQSAARHSHDLLTLISAAMTNHRTCVDGFYNSSGTVRSRVELY 180
           LD SIAEL+ASI DL P QSAARHSHDLLTL+SAAMTNHRTCVDGFYNSSGTVRSRVELY
Sbjct: 121 LDDSIAELKASILDLGPSQSAARHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRVELY 180

Query: 181 LGKIGQHLSIDLAMLKKIPGVNRAPAAGEVLPEYGAVKGGFPNWVSVKDRRLLQAAVNET 240
           LGKIGQHLSIDLAMLKKIPGVNRA    EVLPEYGAVKGGFP WVSVKDRRLLQAAVNET
Sbjct: 181 LGKIGQHLSIDLAMLKKIPGVNRATKVEEVLPEYGAVKGGFPKWVSVKDRRLLQAAVNET 240

Query: 241 KFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERRKTNLMFVGDG 300
           KFNLVVAKDGSGNFTTV EAVAAAPNSSTTRFVIYIKAGAYFENVEI R K+NLMFVGDG
Sbjct: 241 KFNLVVAKDGSGNFTTVNEAVAAAPNSSTTRFVIYIKAGAYFENVEIGRAKSNLMFVGDG 300

Query: 301 IGKTLIKADRNVVDGWTTFRSATVAVVGNGFIAKGITFENYAGPSKHQAVALRSNSDFSA 360
           IGKTLIK DRNVVDGWTTFRSATVAVVG GFIAKGITFENYAGPSKHQAVALRSNSDFSA
Sbjct: 301 IGKTLIKGDRNVVDGWTTFRSATVAVVGTGFIAKGITFENYAGPSKHQAVALRSNSDFSA 360

Query: 361 FYQCSFVGYQDTLYVHSLRQFYRDCDIYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIF 420
           FYQCSF+GYQDTLYVHSLRQFYR+CDIYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIF
Sbjct: 361 FYQCSFIGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIF 420

Query: 421 TAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRTVFLRSFIGQLVD 480
           TAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKL+SRTVFLRSFIGQLV+
Sbjct: 421 TAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLHSRTVFLRSFIGQLVE 480

Query: 481 PAGWLEWNGTFALDTLYYGEYMNRGPGSNTTGRVTWPGYRVITNASEASQFTVEGFIQGS 540
           P GWLEWNGTFALDTLYYGEY+NRGPGSNTTGRVTWPGYRVITNA+EASQFTVE FIQGS
Sbjct: 481 PVGWLEWNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNATEASQFTVEEFIQGS 540

Query: 541 SWLNSTGIPFFSGL 555
           SWLNSTGIPFFSGL
Sbjct: 541 SWLNSTGIPFFSGL 554

BLAST of Bhi09G000758 vs. TrEMBL
Match: tr|A0A0A0K5K8|A0A0A0K5K8_CUCSA (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_7G405320 PE=3 SV=1)

HSP 1 Score: 957.2 bits (2473), Expect = 1.6e-275
Identity = 474/514 (92.22%), Postives = 488/514 (94.94%), Query Frame = 0

Query: 42  HQTARSLCNGTLFPHLCFSKLSSFPQLASLSPEKLAGSAINFTTREVLGAYANCTKLKTI 101
           HQTARSLCNGTLFP LCFSKLSSFPQLASLSPEKLAGSA+NFT+REVL AY NCT LKT 
Sbjct: 98  HQTARSLCNGTLFPDLCFSKLSSFPQLASLSPEKLAGSALNFTSREVLLAYTNCTNLKTH 157

Query: 102 LYGGLNPTDRRALDDCLELLDSSIAELQASICDLAPPQSAARHSHDLLTLISAAMTNHRT 161
           LY GLNPTDR ALDDCLELLD SIAEL+ASI DLAP QS A HSHDLLTL+SAAMTNHRT
Sbjct: 158 LYSGLNPTDRHALDDCLELLDGSIAELKASIFDLAPSQSPALHSHDLLTLVSAAMTNHRT 217

Query: 162 CVDGFYNSSGTVRSRVELYLGKIGQHLSIDLAMLKKIPGVNRAPAAG-EVLPEYGAVKGG 221
           CVDGFYNSSGTVRSRVELYLGKIGQHLSIDLAMLKKIPGVNRA     E+LPEYGAVKGG
Sbjct: 218 CVDGFYNSSGTVRSRVELYLGKIGQHLSIDLAMLKKIPGVNRATGVDQEMLPEYGAVKGG 277

Query: 222 FPNWVSVKDRRLLQAAVNETKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGA 281
           FP WVSVKDRRLLQAAVNETKFN+VVAKDGSGNFTTV EAVAAAPN+STTRFVIYIKAGA
Sbjct: 278 FPKWVSVKDRRLLQAAVNETKFNMVVAKDGSGNFTTVSEAVAAAPNASTTRFVIYIKAGA 337

Query: 282 YFENVEIERRKTNLMFVGDGIGKTLIKADRNVVDGWTTFRSATVAVVGNGFIAKGITFEN 341
           YFENVEI R K+NLMFVGDGIGKTLIKADRNVVDGWTTFRSATVAVVG GFIAKGITFEN
Sbjct: 338 YFENVEIGRAKSNLMFVGDGIGKTLIKADRNVVDGWTTFRSATVAVVGTGFIAKGITFEN 397

Query: 342 YAGPSKHQAVALRSNSDFSAFYQCSFVGYQDTLYVHSLRQFYRDCDIYGTIDFIFGNAAV 401
           YAGPSKHQAVALRSNSDFSAFYQCSF+GYQDTLYVHSLRQFYRDCD+YGTIDFIFGNAAV
Sbjct: 398 YAGPSKHQAVALRSNSDFSAFYQCSFIGYQDTLYVHSLRQFYRDCDVYGTIDFIFGNAAV 457

Query: 402 VFQNCNLYARKPNSNQRNIFTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGR 461
           VFQNCNLYARKPNSNQRNIFTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGR
Sbjct: 458 VFQNCNLYARKPNSNQRNIFTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGR 517

Query: 462 PWKLYSRTVFLRSFIGQLVDPAGWLEWNGTFALDTLYYGEYMNRGPGSNTTGRVTWPGYR 521
           PWKLYSRTVFLRSFIGQL++P GWLEWNGTFALDTLYYGEY+NRGPGSNTT RVTWPGYR
Sbjct: 518 PWKLYSRTVFLRSFIGQLIEPVGWLEWNGTFALDTLYYGEYLNRGPGSNTTMRVTWPGYR 577

Query: 522 VITNASEASQFTVEGFIQGSSWLNSTGIPFFSGL 555
           VITNA+EASQFTVE FIQGSSWLNST IPFFSGL
Sbjct: 578 VITNATEASQFTVERFIQGSSWLNSTEIPFFSGL 611

BLAST of Bhi09G000758 vs. TrEMBL
Match: tr|A0A2I4EZS6|A0A2I4EZS6_9ROSI (Pectinesterase OS=Juglans regia OX=51240 GN=LOC108994228 PE=3 SV=1)

HSP 1 Score: 755.0 bits (1948), Expect = 1.2e-214
Identity = 369/515 (71.65%), Postives = 430/515 (83.50%), Query Frame = 0

Query: 43  QTARSLCNGTLFPHLCFSKLSSFPQLASLSPEKLAGSAINFTTREVLGAYANCTKLKTIL 102
           Q A S C GTL+P LC S LSSFP L S S +++  + +N T  EV  + +NCT ++  L
Sbjct: 50  QVAHSTCQGTLYPDLCVSTLSSFPDLTSKSLQQIISATVNHTIYEVKSSSSNCTGIRKKL 109

Query: 103 YGGLNPTDRRALDDCLELLDSSIAELQASICDL--APPQSAARHSHDLLTLISAAMTNHR 162
              L+P ++RALDDCLEL D++IAEL+ +I DL      SA+++ H+L TL+SAAMTN  
Sbjct: 110 KNLLDPLEKRALDDCLELFDNTIAELRTTISDLFAMRKSSASKNYHNLQTLLSAAMTNQY 169

Query: 163 TCVDGFYNSSGTVRSRVELYLGKIGQHLSIDLAMLKKIPGVNRAPAAGEVLPEYGAVKGG 222
           TC+DGF    G VR  ++  L  I  H+S  LA+LKK+PG+N + +  EV PEYG +K G
Sbjct: 170 TCLDGFAYCKGNVRDEIKNSLYNISHHVSNSLAILKKVPGINSSKS--EVYPEYGKMKNG 229

Query: 223 FPNWVSVKDRRLLQAAVNETKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGA 282
           FP+WV+ KDR+LLQ++VNETK++LVVA DG+GNFTT+G+AVAAAPNSSTTRFVIYIKAGA
Sbjct: 230 FPSWVARKDRKLLQSSVNETKYDLVVATDGTGNFTTIGDAVAAAPNSSTTRFVIYIKAGA 289

Query: 283 YFENVEIERRKTNLMFVGDGIGKTLIKADRNVVDGWTTFRSATVAVVGNGFIAKGITFEN 342
           YFENVE+ER+KTNLMF+GDGIGKT++KA RNVVDGWTTFRSATVAVVG+GFIAKGITFEN
Sbjct: 290 YFENVEVERKKTNLMFIGDGIGKTIVKASRNVVDGWTTFRSATVAVVGDGFIAKGITFEN 349

Query: 343 YAGPSKHQAVALRSNSDFSAFYQCSFVGYQDTLYVHSLRQFYRDCDIYGTIDFIFGNAAV 402
            AGPSKHQAVALRS++D SAFYQCSFV YQD+LYVHSLRQFYRDCDIYGTIDFIFGNAAV
Sbjct: 350 SAGPSKHQAVALRSSADLSAFYQCSFVAYQDSLYVHSLRQFYRDCDIYGTIDFIFGNAAV 409

Query: 403 VFQNCNLYARKPNSNQRNIFTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGR 462
           VFQNCNLYARKPN NQRNIFTAQGREDPNQNTGISILNCKV AASDLIPV SSF TYLGR
Sbjct: 410 VFQNCNLYARKPNENQRNIFTAQGREDPNQNTGISILNCKVTAASDLIPVKSSFETYLGR 469

Query: 463 PWKLYSRTVFLRSFIGQLVDPAGWLEWNGTFALDTLYYGEYMNRGPGSNTTGRVTWPGYR 522
           PWK YSRTVFL S+I  LVDPAGWLEWNGTFAL TLYYGEYMNRGPGSNT+ RVTWPGYR
Sbjct: 470 PWKEYSRTVFLESYIDDLVDPAGWLEWNGTFALSTLYYGEYMNRGPGSNTSARVTWPGYR 529

Query: 523 VITNASEASQFTVEGFIQGSSWLNSTGIPFFSGLA 556
           VI +++EASQFTV  F+QG+ WLNST IP++  L+
Sbjct: 530 VINSSTEASQFTVGAFLQGNEWLNSTVIPYYLNLS 562

BLAST of Bhi09G000758 vs. TrEMBL
Match: tr|A0A0L9VH61|A0A0L9VH61_PHAAN (Pectinesterase OS=Phaseolus angularis OX=3914 GN=LR48_Vigan10g027800 PE=3 SV=1)

HSP 1 Score: 746.1 bits (1925), Expect = 5.6e-212
Identity = 359/513 (69.98%), Postives = 426/513 (83.04%), Query Frame = 0

Query: 43  QTARSLCNGTLFPHLCFSKLSSFPQLASLSPEKLAGSAINFTTREVLGAYANCTKLKTIL 102
           Q A+S C GTL+P LC S L++FP L S S  +L  S +N T  EV  ++ NC+ LK  +
Sbjct: 42  QVAQSTCQGTLYPELCVSTLATFPDLTSKSLPQLISSVVNHTIYEVKSSFYNCSSLKKKI 101

Query: 103 YGGLNPTDRRALDDCLELLDSSIAELQASICDLAPPQSAARHSHDLLTLISAAMTNHRTC 162
              LNP D+RALDDCL+L + +  EL+A+I DL+     ++  HDL TL+S AMTN  TC
Sbjct: 102 -RNLNPLDQRALDDCLKLFEDTNLELKATIDDLSKSTIGSKRHHDLQTLLSGAMTNLYTC 161

Query: 163 VDGFYNSSGTVRSRVELYLGKIGQHLSIDLAMLKKIPGVNRAPAAGEVLPEYGAVKGGFP 222
           +DGF  S G VR R+E  L +I  H+S  LAMLKK+P V +  +  EV PEYG +K GFP
Sbjct: 162 LDGFAYSKGRVRDRIEKKLLEISHHMSNSLAMLKKVPEVKKTTSKSEVFPEYGKMKKGFP 221

Query: 223 NWVSVKDRRLLQAAVNETKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYF 282
           +WVS  DR+LLQA+V+ETKF+LVVAKDG+GNFTT+GEA+A APNSSTTRFVI+IK GAYF
Sbjct: 222 SWVSPNDRKLLQASVDETKFDLVVAKDGTGNFTTIGEALAVAPNSSTTRFVIHIKEGAYF 281

Query: 283 ENVEIERRKTNLMFVGDGIGKTLIKADRNVVDGWTTFRSATVAVVGNGFIAKGITFENYA 342
           ENVE+ R++TNLMFVGDGIGKT++K  RNVVDGWTTF+SATVAVVG GFIAKGITFEN A
Sbjct: 282 ENVEVIRKQTNLMFVGDGIGKTVVKGSRNVVDGWTTFQSATVAVVGEGFIAKGITFENSA 341

Query: 343 GPSKHQAVALRSNSDFSAFYQCSFVGYQDTLYVHSLRQFYRDCDIYGTIDFIFGNAAVVF 402
           GP KHQAVALRS +DFSAF++CSFVGYQDTLYVHSLRQFYR+CDIYGT+DFIFGNAAVVF
Sbjct: 342 GPDKHQAVALRSGADFSAFFRCSFVGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAVVF 401

Query: 403 QNCNLYARKPNSNQRNIFTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPW 462
           QNCNLYARKPN NQ+N+FTAQGREDPNQNTGISILNCK+ AASDLIPV SSF++YLGRPW
Sbjct: 402 QNCNLYARKPNENQKNLFTAQGREDPNQNTGISILNCKIAAASDLIPVKSSFKSYLGRPW 461

Query: 463 KLYSRTVFLRSFIGQLVDPAGWLEWNGTFALDTLYYGEYMNRGPGSNTTGRVTWPGYRVI 522
           K YSRTV+L+S++  L+DPAGWLEWN TFALDTLYYGEYMN+GPGSNT+ RVTWPGYRVI
Sbjct: 462 KTYSRTVYLKSYMEDLIDPAGWLEWNETFALDTLYYGEYMNKGPGSNTSARVTWPGYRVI 521

Query: 523 TNASEASQFTVEGFIQGSSWLNSTGIPFFSGLA 556
            +++EA+QFTV  FIQG+ WLN+TGIPFFSGL+
Sbjct: 522 NSSTEATQFTVAQFIQGNDWLNTTGIPFFSGLS 553

BLAST of Bhi09G000758 vs. TrEMBL
Match: tr|A0A0S3SSE1|A0A0S3SSE1_PHAAN (Pectinesterase OS=Vigna angularis var. angularis OX=157739 GN=Vigan.08G252900 PE=3 SV=1)

HSP 1 Score: 746.1 bits (1925), Expect = 5.6e-212
Identity = 359/513 (69.98%), Postives = 426/513 (83.04%), Query Frame = 0

Query: 43  QTARSLCNGTLFPHLCFSKLSSFPQLASLSPEKLAGSAINFTTREVLGAYANCTKLKTIL 102
           Q A+S C GTL+P LC S L++FP L S S  +L  S +N T  EV  ++ NC+ LK  +
Sbjct: 42  QVAQSTCQGTLYPELCVSTLATFPDLTSKSLPQLISSVVNHTIYEVKSSFYNCSSLKKKI 101

Query: 103 YGGLNPTDRRALDDCLELLDSSIAELQASICDLAPPQSAARHSHDLLTLISAAMTNHRTC 162
              LNP D+RALDDCL+L + +  EL+A+I DL+     ++  HDL TL+S AMTN  TC
Sbjct: 102 -RNLNPLDQRALDDCLKLFEDTNLELKATIDDLSKSTIGSKRHHDLQTLLSGAMTNLYTC 161

Query: 163 VDGFYNSSGTVRSRVELYLGKIGQHLSIDLAMLKKIPGVNRAPAAGEVLPEYGAVKGGFP 222
           +DGF  S G VR R+E  L +I  H+S  LAMLKK+P V +  +  EV PEYG +K GFP
Sbjct: 162 LDGFAYSKGRVRDRIEKKLLEISHHMSNSLAMLKKVPEVKKTTSKSEVFPEYGKMKKGFP 221

Query: 223 NWVSVKDRRLLQAAVNETKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYF 282
           +WVS  DR+LLQA+V+ETKF+LVVAKDG+GNFTT+GEA+A APNSSTTRFVI+IK GAYF
Sbjct: 222 SWVSPNDRKLLQASVDETKFDLVVAKDGTGNFTTIGEALAVAPNSSTTRFVIHIKEGAYF 281

Query: 283 ENVEIERRKTNLMFVGDGIGKTLIKADRNVVDGWTTFRSATVAVVGNGFIAKGITFENYA 342
           ENVE+ R++TNLMFVGDGIGKT++K  RNVVDGWTTF+SATVAVVG GFIAKGITFEN A
Sbjct: 282 ENVEVIRKQTNLMFVGDGIGKTVVKGSRNVVDGWTTFQSATVAVVGEGFIAKGITFENSA 341

Query: 343 GPSKHQAVALRSNSDFSAFYQCSFVGYQDTLYVHSLRQFYRDCDIYGTIDFIFGNAAVVF 402
           GP KHQAVALRS +DFSAF++CSFVGYQDTLYVHSLRQFYR+CDIYGT+DFIFGNAAVVF
Sbjct: 342 GPDKHQAVALRSGADFSAFFRCSFVGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAVVF 401

Query: 403 QNCNLYARKPNSNQRNIFTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPW 462
           QNCNLYARKPN NQ+N+FTAQGREDPNQNTGISILNCK+ AASDLIPV SSF++YLGRPW
Sbjct: 402 QNCNLYARKPNENQKNLFTAQGREDPNQNTGISILNCKIAAASDLIPVKSSFKSYLGRPW 461

Query: 463 KLYSRTVFLRSFIGQLVDPAGWLEWNGTFALDTLYYGEYMNRGPGSNTTGRVTWPGYRVI 522
           K YSRTV+L+S++  L+DPAGWLEWN TFALDTLYYGEYMN+GPGSNT+ RVTWPGYRVI
Sbjct: 462 KTYSRTVYLKSYMEDLIDPAGWLEWNETFALDTLYYGEYMNKGPGSNTSARVTWPGYRVI 521

Query: 523 TNASEASQFTVEGFIQGSSWLNSTGIPFFSGLA 556
            +++EA+QFTV  FIQG+ WLN+TGIPFFSGL+
Sbjct: 522 NSSTEATQFTVAQFIQGNDWLNTTGIPFFSGLS 553

BLAST of Bhi09G000758 vs. NCBI nr
Match: XP_008451545.2 (PREDICTED: pectinesterase-like [Cucumis melo])

HSP 1 Score: 1011.5 bits (2614), Expect = 1.1e-291
Identity = 503/554 (90.79%), Postives = 515/554 (92.96%), Query Frame = 0

Query: 1   MYGLKNRAQIPSNPTXXXXXXXXXXXXXXXXXKPISSNPQIHQTARSLCNGTLFPHLCFS 60
           MYGL+NRAQI SNPT                 KPISSNPQIHQTARSLCNGTLFP+LCFS
Sbjct: 1   MYGLRNRAQISSNPTSISTISTFFFIFFLFSSKPISSNPQIHQTARSLCNGTLFPNLCFS 60

Query: 61  KLSSFPQLASLSPEKLAGSAINFTTREVLGAYANCTKLKTILYGGLNPTDRRALDDCLEL 120
           KLSSFPQLASLSPEKLAGSA+NFTTREVL AYANCT LKT LY GLNPTDR ALDDCLEL
Sbjct: 61  KLSSFPQLASLSPEKLAGSALNFTTREVLLAYANCTNLKTHLYCGLNPTDRHALDDCLEL 120

Query: 121 LDSSIAELQASICDLAPPQSAARHSHDLLTLISAAMTNHRTCVDGFYNSSGTVRSRVELY 180
           LD SIAEL+ASI DL P QSAARHSHDLLTL+SAAMTNHRTCVDGFYNSSGTVRSRVELY
Sbjct: 121 LDDSIAELKASILDLGPSQSAARHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRVELY 180

Query: 181 LGKIGQHLSIDLAMLKKIPGVNRAPAAGEVLPEYGAVKGGFPNWVSVKDRRLLQAAVNET 240
           LGKIGQHLSIDLAMLKKIPGVNRA    EVLPEYGAVKGGFP WVSVKDRRLLQAAVNET
Sbjct: 181 LGKIGQHLSIDLAMLKKIPGVNRATKVEEVLPEYGAVKGGFPKWVSVKDRRLLQAAVNET 240

Query: 241 KFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERRKTNLMFVGDG 300
           KFNLVVAKDGSGNFTTV EAVAAAPNSSTTRFVIYIKAGAYFENVEI R K+NLMFVGDG
Sbjct: 241 KFNLVVAKDGSGNFTTVNEAVAAAPNSSTTRFVIYIKAGAYFENVEIGRAKSNLMFVGDG 300

Query: 301 IGKTLIKADRNVVDGWTTFRSATVAVVGNGFIAKGITFENYAGPSKHQAVALRSNSDFSA 360
           IGKTLIK DRNVVDGWTTFRSATVAVVG GFIAKGITFENYAGPSKHQAVALRSNSDFSA
Sbjct: 301 IGKTLIKGDRNVVDGWTTFRSATVAVVGTGFIAKGITFENYAGPSKHQAVALRSNSDFSA 360

Query: 361 FYQCSFVGYQDTLYVHSLRQFYRDCDIYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIF 420
           FYQCSF+GYQDTLYVHSLRQFYR+CDIYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIF
Sbjct: 361 FYQCSFIGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIF 420

Query: 421 TAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRTVFLRSFIGQLVD 480
           TAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKL+SRTVFLRSFIGQLV+
Sbjct: 421 TAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLHSRTVFLRSFIGQLVE 480

Query: 481 PAGWLEWNGTFALDTLYYGEYMNRGPGSNTTGRVTWPGYRVITNASEASQFTVEGFIQGS 540
           P GWLEWNGTFALDTLYYGEY+NRGPGSNTTGRVTWPGYRVITNA+EASQFTVE FIQGS
Sbjct: 481 PVGWLEWNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNATEASQFTVEEFIQGS 540

Query: 541 SWLNSTGIPFFSGL 555
           SWLNSTGIPFFSGL
Sbjct: 541 SWLNSTGIPFFSGL 554

BLAST of Bhi09G000758 vs. NCBI nr
Match: XP_022991224.1 (pectinesterase-like [Cucurbita maxima])

HSP 1 Score: 999.2 bits (2582), Expect = 5.5e-288
Identity = 493/556 (88.67%), Postives = 514/556 (92.45%), Query Frame = 0

Query: 1   MYGLKNRAQIPSNPT-XXXXXXXXXXXXXXXXXKPISSNPQIHQTARSLCNGTLFPHLCF 60
           MYGL++RAQ  SNPT                  KPISSNPQ HQTARSLCNGTLFP+LCF
Sbjct: 1   MYGLRSRAQSSSNPTASISAIFTLFSVLFLFSFKPISSNPQTHQTARSLCNGTLFPNLCF 60

Query: 61  SKLSSFPQLASLSPEKLAGSAINFTTREVLGAYANCTKLKTILYGGLNPTDRRALDDCLE 120
           SKLSSFPQLASLSPEKLAGSA+NFT  EV GAYANC+KLKT+ YGGLNPTDRRALDDCLE
Sbjct: 61  SKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLNPTDRRALDDCLE 120

Query: 121 LLDSSIAELQASICDLAPPQSAARHSHDLLTLISAAMTNHRTCVDGFYNSSGTVRSRVEL 180
           LLD SIAELQ SI DL+P QSAARHSHDLLTL+SAAMTNHRTC+DGFYNSSGTVR RVEL
Sbjct: 121 LLDGSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVEL 180

Query: 181 YLGKIGQHLSIDLAMLKKIPGVNRAPAAGEVLPEYGAVKGGFPNWVSVKDRRLLQAAVNE 240
           YLGKI QHLSIDLAMLKKIPGV RA  A EVLPEYGAV+GGFP WVSVKDRRLLQAAVNE
Sbjct: 181 YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNE 240

Query: 241 TKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERRKTNLMFVGD 300
           TKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIER+KTNLMFVGD
Sbjct: 241 TKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGD 300

Query: 301 GIGKTLIKADRNVVDGWTTFRSATVAVVGNGFIAKGITFENYAGPSKHQAVALRSNSDFS 360
           GIGKTLIKADRNVVDGWTTFRSATVA VGNGFIAKGITFEN+AGPSKHQAVA RSNSDFS
Sbjct: 301 GIGKTLIKADRNVVDGWTTFRSATVAAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFS 360

Query: 361 AFYQCSFVGYQDTLYVHSLRQFYRDCDIYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNI 420
           AFYQCSF+GYQDTLYVHSLRQFYR+CD+YGTIDFIFGNAAVVFQNCNLYARKPNSNQRNI
Sbjct: 361 AFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNI 420

Query: 421 FTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRTVFLRSFIGQLV 480
           FTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRT+FL+SFIGQLV
Sbjct: 421 FTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRTIFLKSFIGQLV 480

Query: 481 DPAGWLEWNGTFALDTLYYGEYMNRGPGSNTTGRVTWPGYRVITNASEASQFTVEGFIQG 540
           +P GWLEWNGTFALDTLYYGEY+NRGPGSNTTGRVTWPGYRVITN++EASQFTVE FIQG
Sbjct: 481 EPVGWLEWNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQG 540

Query: 541 SSWLNSTGIPFFSGLA 556
           S WLNST IPFFSGLA
Sbjct: 541 SEWLNSTEIPFFSGLA 556

BLAST of Bhi09G000758 vs. NCBI nr
Match: XP_023548978.1 (pectinesterase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 991.9 bits (2563), Expect = 8.9e-286
Identity = 489/556 (87.95%), Postives = 513/556 (92.27%), Query Frame = 0

Query: 1   MYGLKNRAQIPSNPT-XXXXXXXXXXXXXXXXXKPISSNPQIHQTARSLCNGTLFPHLCF 60
           MYGL+NRAQ  SNPT                  KPISSNPQ HQTARSLCNGTLFP+LCF
Sbjct: 1   MYGLRNRAQSSSNPTASISAIFTFFSVLILCSFKPISSNPQTHQTARSLCNGTLFPNLCF 60

Query: 61  SKLSSFPQLASLSPEKLAGSAINFTTREVLGAYANCTKLKTILYGGLNPTDRRALDDCLE 120
           SKLSSFPQLASLSPEKLAGSA+NFT  EV GAYANC+KLKT+ YGGL+PTDRRALDDCLE
Sbjct: 61  SKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLSPTDRRALDDCLE 120

Query: 121 LLDSSIAELQASICDLAPPQSAARHSHDLLTLISAAMTNHRTCVDGFYNSSGTVRSRVEL 180
           LLD SIAELQ SI DL+P QSAARHSHDLLTL+SAAMTNHRTC+DGFYNSSGTVR RVEL
Sbjct: 121 LLDGSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVEL 180

Query: 181 YLGKIGQHLSIDLAMLKKIPGVNRAPAAGEVLPEYGAVKGGFPNWVSVKDRRLLQAAVNE 240
           YLGKI QHLSIDLAMLKKIPGV RA  A EVLPEYGAV+GGFP WVSVKDRRLLQAAVNE
Sbjct: 181 YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNE 240

Query: 241 TKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERRKTNLMFVGD 300
           TKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIER+KTNL+FVGD
Sbjct: 241 TKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLVFVGD 300

Query: 301 GIGKTLIKADRNVVDGWTTFRSATVAVVGNGFIAKGITFENYAGPSKHQAVALRSNSDFS 360
           GIGKTLIKA+RNVVDGWTTFRSATVA VGNGFIAKGITFEN+AGPSKHQAVA RSNSDFS
Sbjct: 301 GIGKTLIKANRNVVDGWTTFRSATVAAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFS 360

Query: 361 AFYQCSFVGYQDTLYVHSLRQFYRDCDIYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNI 420
           AFYQCSF+GYQDTLYVHSLRQFYR+CD+YGT+DFIFGNAAVVFQNCNLYARKPNSNQRNI
Sbjct: 361 AFYQCSFIGYQDTLYVHSLRQFYRECDVYGTVDFIFGNAAVVFQNCNLYARKPNSNQRNI 420

Query: 421 FTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRTVFLRSFIGQLV 480
           FTAQGREDPNQNTGISILNCKVEAASDLIPVL SFRTYLGRPWKLYSRTVFL+SFIGQLV
Sbjct: 421 FTAQGREDPNQNTGISILNCKVEAASDLIPVLPSFRTYLGRPWKLYSRTVFLKSFIGQLV 480

Query: 481 DPAGWLEWNGTFALDTLYYGEYMNRGPGSNTTGRVTWPGYRVITNASEASQFTVEGFIQG 540
           +P GWLEW+GTFALDTLYYGEY+NRGPGSNTTGRVTWPGYRVITN++EASQFTVE FIQG
Sbjct: 481 EPVGWLEWSGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQG 540

Query: 541 SSWLNSTGIPFFSGLA 556
           S WLNST IPFFSGLA
Sbjct: 541 SEWLNSTEIPFFSGLA 556

BLAST of Bhi09G000758 vs. NCBI nr
Match: XP_004136014.1 (PREDICTED: pectinesterase-like [Cucumis sativus] >XP_011659370.1 PREDICTED: pectinesterase-like [Cucumis sativus])

HSP 1 Score: 990.3 bits (2559), Expect = 2.6e-285
Identity = 510/558 (91.40%), Postives = 526/558 (94.27%), Query Frame = 0

Query: 1   MYGLKNRAQIPSNPT---XXXXXXXXXXXXXXXXXKPISSNPQIHQTARSLCNGTLFPHL 60
           MYGL+NRAQI SNPT   XXXXXXXXXXXXXXX  KPISSNPQ+HQTARSLCNGTLFP L
Sbjct: 1   MYGLRNRAQISSNPTSIXXXXXXXXXXXXXXXXSFKPISSNPQVHQTARSLCNGTLFPDL 60

Query: 61  CFSKLSSFPQLASLSPEKLAGSAINFTTREVLGAYANCTKLKTILYGGLNPTDRRALDDC 120
           CFSKLSSFPQLASLSPEKLAGSA+NFT+REVL AY NCT LKT LY GLNPTDR ALDDC
Sbjct: 61  CFSKLSSFPQLASLSPEKLAGSALNFTSREVLLAYTNCTNLKTHLYSGLNPTDRHALDDC 120

Query: 121 LELLDSSIAELQASICDLAPPQSAARHSHDLLTLISAAMTNHRTCVDGFYNSSGTVRSRV 180
           LELLD SIAEL+ASI DLAP QS A HSHDLLTL+SAAMTNHRTCVDGFYNSSGTVRSRV
Sbjct: 121 LELLDGSIAELKASIFDLAPSQSPALHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRV 180

Query: 181 ELYLGKIGQHLSIDLAMLKKIPGVNRAPAAG-EVLPEYGAVKGGFPNWVSVKDRRLLQAA 240
           ELYLGKIGQHLSIDLAMLKKIPGVNRA     E+LPEYGAVKGGFP WVSVKDRRLLQAA
Sbjct: 181 ELYLGKIGQHLSIDLAMLKKIPGVNRATGVDQEMLPEYGAVKGGFPKWVSVKDRRLLQAA 240

Query: 241 VNETKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERRKTNLMF 300
           VNETKFN+VVAKDGSGNFTTV EAVAAAPN+STTRFVIYIKAGAYFENVEI R K+NLMF
Sbjct: 241 VNETKFNMVVAKDGSGNFTTVSEAVAAAPNASTTRFVIYIKAGAYFENVEIGRAKSNLMF 300

Query: 301 VGDGIGKTLIKADRNVVDGWTTFRSATVAVVGNGFIAKGITFENYAGPSKHQAVALRSNS 360
           VGDGIGKTLIKADRNVVDGWTTFRSATVAVVG GFIAKGITFENYAGPSKHQAVALRSNS
Sbjct: 301 VGDGIGKTLIKADRNVVDGWTTFRSATVAVVGTGFIAKGITFENYAGPSKHQAVALRSNS 360

Query: 361 DFSAFYQCSFVGYQDTLYVHSLRQFYRDCDIYGTIDFIFGNAAVVFQNCNLYARKPNSNQ 420
           DFSAFYQCSF+GYQDTLYVHSLRQFYRDCD+YGTIDFIFGNAAVVFQNCNLYARKPNSNQ
Sbjct: 361 DFSAFYQCSFIGYQDTLYVHSLRQFYRDCDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQ 420

Query: 421 RNIFTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRTVFLRSFIG 480
           RNIFTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRTVFLRSFIG
Sbjct: 421 RNIFTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRTVFLRSFIG 480

Query: 481 QLVDPAGWLEWNGTFALDTLYYGEYMNRGPGSNTTGRVTWPGYRVITNASEASQFTVEGF 540
           QL++P GWLEWNGTFALDTLYYGEY+NRGPGSNTT RVTWPGYRVITNA+EASQFTVE F
Sbjct: 481 QLIEPVGWLEWNGTFALDTLYYGEYLNRGPGSNTTMRVTWPGYRVITNATEASQFTVERF 540

Query: 541 IQGSSWLNSTGIPFFSGL 555
           IQGSSWLNST IPFFSGL
Sbjct: 541 IQGSSWLNSTEIPFFSGL 558

BLAST of Bhi09G000758 vs. NCBI nr
Match: XP_022953706.1 (pectinesterase-like [Cucurbita moschata])

HSP 1 Score: 989.6 bits (2557), Expect = 4.4e-285
Identity = 487/556 (87.59%), Postives = 511/556 (91.91%), Query Frame = 0

Query: 1   MYGLKNRAQIPSNPT-XXXXXXXXXXXXXXXXXKPISSNPQIHQTARSLCNGTLFPHLCF 60
           MYGL++RAQ  SNPT                  KPISSNPQ HQTARSLCNGTLFP+LCF
Sbjct: 1   MYGLRSRAQSSSNPTASISAIFTFFSVLFLCSFKPISSNPQTHQTARSLCNGTLFPNLCF 60

Query: 61  SKLSSFPQLASLSPEKLAGSAINFTTREVLGAYANCTKLKTILYGGLNPTDRRALDDCLE 120
           SKLSSFPQLASLSPEKLAGSA+NFT  EV GAYANC+KLKT+ YGGLNPTDRRALDDCLE
Sbjct: 61  SKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLNPTDRRALDDCLE 120

Query: 121 LLDSSIAELQASICDLAPPQSAARHSHDLLTLISAAMTNHRTCVDGFYNSSGTVRSRVEL 180
           LLD SIAELQ SI DL+P QSAARHSHDLLTL+SAAMTNHRTC+DGFYNSSGTVR RVEL
Sbjct: 121 LLDGSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVEL 180

Query: 181 YLGKIGQHLSIDLAMLKKIPGVNRAPAAGEVLPEYGAVKGGFPNWVSVKDRRLLQAAVNE 240
           YLGKI QHLSIDLAMLKKIPGV RA  A EVLPEYGAV+GGFP WVSVKDRRLLQA+VNE
Sbjct: 181 YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQASVNE 240

Query: 241 TKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERRKTNLMFVGD 300
           TKFNLVVAKDGSGNFTTVG AVAAAPNSSTTRFVIYIKAGAYFENVEIER+KTNLMFVGD
Sbjct: 241 TKFNLVVAKDGSGNFTTVGAAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGD 300

Query: 301 GIGKTLIKADRNVVDGWTTFRSATVAVVGNGFIAKGITFENYAGPSKHQAVALRSNSDFS 360
           GIGKTLIKADRNVVDGWTTFRSATV  VGNGFIAKGITFEN+AGPSKHQAVA RSNSDFS
Sbjct: 301 GIGKTLIKADRNVVDGWTTFRSATVVAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFS 360

Query: 361 AFYQCSFVGYQDTLYVHSLRQFYRDCDIYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNI 420
           AFYQCSF+GYQDTLYVHSLRQFYR+CD+YGTIDFIFGNAAV+FQNCNLYARKPNSNQRNI
Sbjct: 361 AFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVIFQNCNLYARKPNSNQRNI 420

Query: 421 FTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRTVFLRSFIGQLV 480
           FTAQGREDPNQNTGISILNCKVEAASDL+PVL SFRTYLGRPWKLYSRTVFL+SFIGQLV
Sbjct: 421 FTAQGREDPNQNTGISILNCKVEAASDLLPVLPSFRTYLGRPWKLYSRTVFLKSFIGQLV 480

Query: 481 DPAGWLEWNGTFALDTLYYGEYMNRGPGSNTTGRVTWPGYRVITNASEASQFTVEGFIQG 540
           +P GWLEW+GTFALDTLYYGEY+NRGPGSNTTGRVTWPGYRVITN++EASQFTVE FIQG
Sbjct: 481 EPVGWLEWSGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQG 540

Query: 541 SSWLNSTGIPFFSGLA 556
           S WLNST IPFFSGLA
Sbjct: 541 SEWLNSTEIPFFSGLA 556