Cp4.1LG10g03620 (gene) Cucurbita pepo (Zucchini)

BLAST of Cp4.1LG10g03620 vs. Swiss-Prot
Match: GAO1B_WHEAT (Gibberellin 20 oxidase 1-B OS=Triticum aestivum GN=GA20ox1B PE=2 SV=1)

HSP 1 Score: 396.0 bits (1016), Expect = 4.5e-109
Identity = 186/338 (55.03%), Postives = 229/338 (67.75%), Query Frame = 1

Query: 18  NLPTEFVWPHADLAAIVDDPEELQEPLIDLAGFRRGDAAATADAAAMIRVACRNHGLFQV 77
           ++P++F+WP  + +   D  EEL  PLID+ G   GD  ATA+   ++  AC  HG FQV
Sbjct: 16  DIPSQFIWPEGE-SPTPDAAEELHVPLIDIGGMLSGDPRATAEVTRLVGEACERHGFFQV 75

Query: 78  TNHGVDPDLIRAAYEQVEMIFKMPLGKKKSVGKKAGRVCGYSGAHADRFSSKLPWKETFS 137
            NHG+D +L+  A+  V+  F MPL +K+   ++ G  CGY+ +   RF+SKLPWKET S
Sbjct: 76  VNHGIDAELLADAHRCVDAFFTMPLPEKQRALRRPGESCGYASSFTGRFASKLPWKETLS 135

Query: 138 FEYCSDDSQRLHVLHYFKSFLGQDFEKTGWVYQKYCEEMTRTGLMIMELLAISLGVERLH 197
           F  C  D     V+ Y  + LG+D  + G VY +YC EM+R  L IME+L  SLGV R H
Sbjct: 136 FRSCPSDPAL--VVDYIVATLGEDHRRLGEVYARYCSEMSRLSLEIMEVLGESLGVGRAH 195

Query: 198 YRKFFEDGRSIMRCNYYPPCENAALTFGTGPHCDPTSLTILHQDQVGGLEVFANNTWLSV 257
           YR+FFE   SIMR NYYPPC+    T GTGPHCDPTSLTILHQD VGGL+V     W S+
Sbjct: 196 YRRFFEGNDSIMRLNYYPPCQRPMETLGTGPHCDPTSLTILHQDNVGGLQVHTEGRWRSI 255

Query: 258 KPRPDALVINIGDTFMALSNGAYKSCLHRAVVNRKRVRRSLVFFVCPKDDKVVQPPRDLV 317
           +PR DA V+NIGDTFMALSNG YKSCLHRAVVN K  R+SL FF+CP+ DKVV PP  LV
Sbjct: 256 RPRADAFVVNIGDTFMALSNGRYKSCLHRAVVNSKVPRKSLAFFLCPEMDKVVAPPGTLV 315

Query: 318 GREGPRQYPDFTWSDLLEFTQKHYRADVATLQSFVHWL 356
               PR YPDFTW  LL+FTQKHYRAD+ TL+ F  W+
Sbjct: 316 DAANPRAYPDFTWRSLLDFTQKHYRADMKTLEVFSSWI 350

BLAST of Cp4.1LG10g03620 vs. Swiss-Prot
Match: GAO1A_WHEAT (Gibberellin 20 oxidase 1-A OS=Triticum aestivum GN=GA20ox1A PE=2 SV=1)

HSP 1 Score: 394.8 bits (1013), Expect = 1.0e-108
Identity = 185/338 (54.73%), Postives = 230/338 (68.05%), Query Frame = 1

Query: 18  NLPTEFVWPHADLAAIVDDPEELQEPLIDLAGFRRGDAAATADAAAMIRVACRNHGLFQV 77
           ++P++F+WP  + +   D  EEL  PLI++ G   GDAAA A+   ++  AC  HG FQV
Sbjct: 16  DIPSQFIWPEGE-SPTPDAAEELHVPLINIGGMLSGDAAAAAEVTRLVGEACERHGFFQV 75

Query: 78  TNHGVDPDLIRAAYEQVEMIFKMPLGKKKSVGKKAGRVCGYSGAHADRFSSKLPWKETFS 137
            NHG+D +L+  A+  V+  F MPL +K+   ++ G  CGY+ +   RF+SKLPWKET S
Sbjct: 76  VNHGIDAELLADAHRCVDNFFTMPLPEKQRALRRPGESCGYASSFTGRFASKLPWKETLS 135

Query: 138 FEYCSDDSQRLHVLHYFKSFLGQDFEKTGWVYQKYCEEMTRTGLMIMELLAISLGVERLH 197
           F  C  D     V+ Y  + LG+D  + G VY +YC EM+R  L IME+L  SLGV R H
Sbjct: 136 FRSCPSDPAL--VVDYIVATLGEDHRRLGEVYARYCSEMSRLSLEIMEVLGESLGVGRAH 195

Query: 198 YRKFFEDGRSIMRCNYYPPCENAALTFGTGPHCDPTSLTILHQDQVGGLEVFANNTWLSV 257
           YR+FFE   SIMR NYYPPC+    T GTGPHCDPTSLTILHQD VGGL+V     W S+
Sbjct: 196 YRRFFEGNDSIMRLNYYPPCQRPLETLGTGPHCDPTSLTILHQDNVGGLQVHTEGRWRSI 255

Query: 258 KPRPDALVINIGDTFMALSNGAYKSCLHRAVVNRKRVRRSLVFFVCPKDDKVVQPPRDLV 317
           +PR DA V+NIGDTFMALSNG YKSCLHRAVVN +  R+SL FF+CP+ DKVV PP  LV
Sbjct: 256 RPRADAFVVNIGDTFMALSNGRYKSCLHRAVVNSRVPRKSLAFFLCPEMDKVVAPPGTLV 315

Query: 318 GREGPRQYPDFTWSDLLEFTQKHYRADVATLQSFVHWL 356
               PR YPDFTW  LL+FTQKHYRAD+ TL+ F  W+
Sbjct: 316 DASNPRAYPDFTWRSLLDFTQKHYRADMKTLEVFSSWI 350

BLAST of Cp4.1LG10g03620 vs. Swiss-Prot
Match: GAO1D_WHEAT (Gibberellin 20 oxidase 1-D OS=Triticum aestivum GN=GA20ox1D PE=1 SV=1)

HSP 1 Score: 393.3 bits (1009), Expect = 2.9e-108
Identity = 185/338 (54.73%), Postives = 228/338 (67.46%), Query Frame = 1

Query: 18  NLPTEFVWPHADLAAIVDDPEELQEPLIDLAGFRRGDAAATADAAAMIRVACRNHGLFQV 77
           ++P++F+WP  + +   D  EEL  PLID+ G   GD AA A+   ++  AC  HG FQV
Sbjct: 16  DIPSQFIWPEGE-SPTPDAAEELHVPLIDIGGMLSGDPAAAAEVTRLVGEACERHGFFQV 75

Query: 78  TNHGVDPDLIRAAYEQVEMIFKMPLGKKKSVGKKAGRVCGYSGAHADRFSSKLPWKETFS 137
            NHG+D +L+  A+  V+  F MPL +K+   +  G  CGY+ +   RF+SKLPWKET S
Sbjct: 76  VNHGIDAELLADAHRCVDNFFTMPLPEKQRALRHPGESCGYASSFTGRFASKLPWKETLS 135

Query: 138 FEYCSDDSQRLHVLHYFKSFLGQDFEKTGWVYQKYCEEMTRTGLMIMELLAISLGVERLH 197
           F  C  D     V+ Y  + LG+D  + G VY +YC EM+R  L IME+L  SLGV R H
Sbjct: 136 FRSCPSDPAL--VVDYIVATLGEDHRRLGEVYARYCSEMSRLSLEIMEVLGESLGVGRAH 195

Query: 198 YRKFFEDGRSIMRCNYYPPCENAALTFGTGPHCDPTSLTILHQDQVGGLEVFANNTWLSV 257
           YR+FFE   SIMR NYYPPC+    T GTGPHCDPTSLTILHQD VGGL+V     W S+
Sbjct: 196 YRRFFEGNDSIMRLNYYPPCQRPLETLGTGPHCDPTSLTILHQDNVGGLQVHTEGRWRSI 255

Query: 258 KPRPDALVINIGDTFMALSNGAYKSCLHRAVVNRKRVRRSLVFFVCPKDDKVVQPPRDLV 317
           +PR DA V+NIGDTFMALSNG YKSCLHRAVVN +  R+SL FF+CP+ DKVV PP  LV
Sbjct: 256 RPRADAFVVNIGDTFMALSNGRYKSCLHRAVVNSRVPRKSLAFFLCPEMDKVVAPPGTLV 315

Query: 318 GREGPRQYPDFTWSDLLEFTQKHYRADVATLQSFVHWL 356
               PR YPDFTW  LL+FTQKHYRAD+ TL+ F  W+
Sbjct: 316 DAANPRAYPDFTWRSLLDFTQKHYRADMKTLEVFSSWI 350

BLAST of Cp4.1LG10g03620 vs. Swiss-Prot
Match: GAOX1_ARATH (Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana GN=GA20OX1 PE=2 SV=2)

HSP 1 Score: 392.9 bits (1008), Expect = 3.8e-108
Identity = 190/346 (54.91%), Postives = 235/346 (67.92%), Query Frame = 1

Query: 10  PPTLLHNGNLPTEFVWPHADLAAIVDDPEELQEPLIDLAGFRRGDAAATADAAAMIRVAC 69
           P  L    N+P +F+WP  +  +I  +  EL  PLIDL      D ++T DA+ +I  AC
Sbjct: 31  PSMLNLQANIPNQFIWPDDEKPSI--NVLELDVPLIDLQNLL-SDPSSTLDASRLISEAC 90

Query: 70  RNHGLFQVTNHGVDPDLIRAAYEQVEMIFKMPLGKKKSVGKKAGRVCGYSGAHADRFSSK 129
           + HG F V NHG+  +LI  A+E     F MPL +K+ V +K+G   GY+ +   RFS+K
Sbjct: 91  KKHGFFLVVNHGISEELISDAHEYTSRFFDMPLSEKQRVLRKSGESVGYASSFTGRFSTK 150

Query: 130 LPWKETFSFEYCSDDSQRLHVLHYFKSFLGQDFEKTGWVYQKYCEEMTRTGLMIMELLAI 189
           LPWKET SF +C D S+   V  YF   LG  F+  G VYQ+YCE M+   L IMELL +
Sbjct: 151 LPWKETLSFRFCDDMSRSKSVQDYFCDALGHGFQPFGKVYQEYCEAMSSLSLKIMELLGL 210

Query: 190 SLGVERLHYRKFFEDGRSIMRCNYYPPCENAALTFGTGPHCDPTSLTILHQDQVGGLEVF 249
           SLGV+R ++R+FFE+  SIMR NYYPPC    LT GTGPHCDPTSLTILHQD V GL+VF
Sbjct: 211 SLGVKRDYFREFFEENDSIMRLNYYPPCIKPDLTLGTGPHCDPTSLTILHQDHVNGLQVF 270

Query: 250 ANNTWLSVKPRPDALVINIGDTFMALSNGAYKSCLHRAVVNRKRVRRSLVFFVCPKDDKV 309
             N W S++P P A V+NIGDTFMALSN  YKSCLHRAVVN +  R+SL FF+CPK D+V
Sbjct: 271 VENQWRSIRPNPKAFVVNIGDTFMALSNDRYKSCLHRAVVNSESERKSLAFFLCPKKDRV 330

Query: 310 VQPPRDLVGREGPRQYPDFTWSDLLEFTQKHYRADVATLQSFVHWL 356
           V PPR+L+     R+YPDFTWS  LEFTQKHYRAD+ TLQ+F  WL
Sbjct: 331 VTPPRELLDSITSRRYPDFTWSMFLEFTQKHYRADMNTLQAFSDWL 373

BLAST of Cp4.1LG10g03620 vs. Swiss-Prot
Match: GAOX2_ORYSJ (Gibberellin 20 oxidase 2 OS=Oryza sativa subsp. japonica GN=20ox2 PE=1 SV=1)

HSP 1 Score: 392.1 bits (1006), Expect = 6.5e-108
Identity = 192/337 (56.97%), Postives = 227/337 (67.36%), Query Frame = 1

Query: 19  LPTEFVWPHADLAAIVDDPEELQEPLIDLAGFRRGDAAATADAAAMIRVACRNHGLFQVT 78
           +P  FVWP+ D  A      EL  P++D+   R GDA     AAA +  AC  HG FQV+
Sbjct: 42  IPEPFVWPNGD--ARPASAAELDMPVVDVGVLRDGDAEGLRRAAAQVAAACATHGFFQVS 101

Query: 79  NHGVDPDLIRAAYEQVEMIFKMPLGKKKSVGKKAGRVCGYSGAHADRFSSKLPWKETFSF 138
            HGVD  L RAA +     F++PL +K+   +  G V GY+ AHADRF+SKLPWKET SF
Sbjct: 102 EHGVDAALARAALDGASDFFRLPLAEKRRARRVPGTVSGYTSAHADRFASKLPWKETLSF 161

Query: 139 EYCSDDSQRLHVLHYFKSFLGQDFEKTGWVYQKYCEEMTRTGLMIMELLAISLGVERLHY 198
            +  D +    V  YF S LG DF   G VYQKYCEEM    L IMELL +SLGVER +Y
Sbjct: 162 GF-HDRAAAPVVADYFSSTLGPDFAPMGRVYQKYCEEMKELSLTIMELLELSLGVERGYY 221

Query: 199 RKFFEDGRSIMRCNYYPPCENAALTFGTGPHCDPTSLTILHQDQVGGLEVFANNTWLSVK 258
           R+FF D  SIMRCNYYPPC     T GTGPHCDPT+LTIL QD VGGLEV  +  W  V 
Sbjct: 222 REFFADSSSIMRCNYYPPCPEPERTLGTGPHCDPTALTILLQDDVGGLEVLVDGEWRPVS 281

Query: 259 PRPDALVINIGDTFMALSNGAYKSCLHRAVVNRKRVRRSLVFFVCPKDDKVVQPPRDLVG 318
           P P A+VINIGDTFMALSNG YKSCLHRAVVN++R RRSL FF+CP++D+VV+PP     
Sbjct: 282 PVPGAMVINIGDTFMALSNGRYKSCLHRAVVNQRRERRSLAFFLCPREDRVVRPPPSAA- 341

Query: 319 REGPRQYPDFTWSDLLEFTQKHYRADVATLQSFVHWL 356
              P+ YPDFTW+DL+ FTQ+HYRAD  TL +F  WL
Sbjct: 342 --TPQHYPDFTWADLMRFTQRHYRADTRTLDAFTRWL 372

BLAST of Cp4.1LG10g03620 vs. TrEMBL
Match: S0BDZ8_CUCSA (GA 20-oxidase OS=Cucumis sativus GN=GA20ox2 PE=2 SV=1)

HSP 1 Score: 606.3 bits (1562), Expect = 2.5e-170
Identity = 288/359 (80.22%), Postives = 314/359 (87.47%), Query Frame = 1

Query: 1   MGSTGTAAIPPTLLHNGNLPTEFVWPHADLAAIVDDPEELQEPLIDLAGFRRGDAAATAD 60
           +G      +P  +     LP EFVWP  +LA   ++  EL+EPLIDL GFRRG+  ATA+
Sbjct: 7   VGRVAGIILPTMVDKKKKLPREFVWPRGELAG--EERGELKEPLIDLGGFRRGEEEATAE 66

Query: 61  AAAMIRVACRNHGLFQVTNHGVDPDLIRAAYEQVEMIFKMPLGKKKSVGKKAGRVCGYSG 120
           AAAM+R+AC  HG+FQVTNHGV+ +LI+AAYE+ E IFKMPL KK SVGKK GRV GYSG
Sbjct: 67  AAAMVRMACMKHGVFQVTNHGVEEELIKAAYEEGEGIFKMPLVKKISVGKKPGRVSGYSG 126

Query: 121 AHADRFSSKLPWKETFSFEYCSDDSQRLHVLHYFKSFLGQDFEKTGWVYQKYCEEMTRTG 180
           AHADRFSSKLPWKETFSFEY +DDSQ LHVLH+FKS  G DFE TGWVYQ+YCEEMTRT 
Sbjct: 127 AHADRFSSKLPWKETFSFEYSNDDSQPLHVLHHFKSLFGCDFENTGWVYQRYCEEMTRTA 186

Query: 181 LMIMELLAISLGVERLHYRKFFEDGRSIMRCNYYPPCENAALTFGTGPHCDPTSLTILHQ 240
           LMIMELLAISLGVER HYRKFFEDG+SIMRCNYYPPCENA+LT GTGPHCDPTSLTILHQ
Sbjct: 187 LMIMELLAISLGVERYHYRKFFEDGKSIMRCNYYPPCENASLTLGTGPHCDPTSLTILHQ 246

Query: 241 DQVGGLEVFANNTWLSVKPRPDALVINIGDTFMALSNGAYKSCLHRAVVNRKRVRRSLVF 300
           DQVGGLEVFANN WLSVKPRPDALVINIGDTFMALSNGAYKSCLHRAVVNRKR RRSLVF
Sbjct: 247 DQVGGLEVFANNAWLSVKPRPDALVINIGDTFMALSNGAYKSCLHRAVVNRKRERRSLVF 306

Query: 301 FVCPKDDKVVQPPRDLVGREGPRQYPDFTWSDLLEFTQKHYRADVATLQSFVHWLRSSP 360
           FVCPKDDKVV+PP+DLVGREGPRQYPDFTWS+LLEFTQKHYRADVATLQSFVHWL++ P
Sbjct: 307 FVCPKDDKVVRPPQDLVGREGPRQYPDFTWSELLEFTQKHYRADVATLQSFVHWLQAKP 363

BLAST of Cp4.1LG10g03620 vs. TrEMBL
Match: B9HEX6_POPTR (Gibberellin 20-oxidase family protein OS=Populus trichocarpa GN=POPTR_0007s04360g PE=3 SV=1)

HSP 1 Score: 531.2 bits (1367), Expect = 1.0e-147
Identity = 252/350 (72.00%), Postives = 286/350 (81.71%), Query Frame = 1

Query: 13  LLHNGNLPTEFVWPHADLAAIVDDPEELQEPLIDLAGFRRGDAAATADAAAMIRVACRNH 72
           L    +LPTEF+WPH D   +V + +EL+EP+IDL GF +GD  ATA AA ++R AC NH
Sbjct: 31  LQKQASLPTEFMWPHGD---LVHNEDELKEPMIDLEGFLKGDEVATAQAAELVRTACLNH 90

Query: 73  GLFQVTNHGVDPDLIRAAYEQVEMIFKMPLGKKKSVGKKAGRVCGYSGAHADRFSSKLPW 132
           G FQVTNHGVD  LI +A+E++  IFK+PL KK SV +K G V GYSGAHA R+SSKLPW
Sbjct: 91  GFFQVTNHGVDISLIHSAHEEIGKIFKLPLDKKLSVRRKPGDVSGYSGAHAHRYSSKLPW 150

Query: 133 KETFSFEYCSDDSQRLHVLHYFKSFLGQDFEKTGWVYQKYCEEMTRTGLMIMELLAISLG 192
           KETFSF Y  DD     V+ YFKS LG+DFE TGWVYQ YCE M +  L+I ELLAISLG
Sbjct: 151 KETFSFGYHGDDDSVPLVVDYFKSVLGKDFEHTGWVYQSYCEAMKKVSLVIFELLAISLG 210

Query: 193 VERLHYRKFFEDGRSIMRCNYYPPCENAALTFGTGPHCDPTSLTILHQDQVGGLEVFANN 252
           V+RLHYRKFFEDG SIMRCNYYPPC N+ LT GTGPHCDPTSLTILHQDQVGGL+VF+NN
Sbjct: 211 VDRLHYRKFFEDGSSIMRCNYYPPCNNSTLTLGTGPHCDPTSLTILHQDQVGGLQVFSNN 270

Query: 253 TWLSVKPRPDALVINIGDTFMALSNGAYKSCLHRAVVNRKRVRRSLVFFVCPKDDKVVQP 312
            WL+++PRPDALV+NIGDTFMALSNG YKSCLHRAVVNR   RRSLVFFV PK++KVV+P
Sbjct: 271 KWLAIRPRPDALVVNIGDTFMALSNGRYKSCLHRAVVNRDSERRSLVFFVSPKEEKVVRP 330

Query: 313 PRDLVGREGPRQYPDFTWSDLLEFTQKHYRADVATLQSFVHWLRSS-PST 362
           P+DLV REG R YPDFTWSDLLEFTQKHYRADVATLQSF+ WL SS PST
Sbjct: 331 PQDLVSREGQRIYPDFTWSDLLEFTQKHYRADVATLQSFIQWLSSSKPST 377

BLAST of Cp4.1LG10g03620 vs. TrEMBL
Match: B9H4B0_POPTR (Gibberellin 20-oxidase family protein OS=Populus trichocarpa GN=POPTR_0005s06660g PE=3 SV=1)

HSP 1 Score: 524.6 bits (1350), Expect = 9.4e-146
Identity = 246/346 (71.10%), Postives = 284/346 (82.08%), Query Frame = 1

Query: 13  LLHNGNLPTEFVWPHADLAAIVDDPEELQEPLIDLAGFRRGDAAATADAAAMIRVACRNH 72
           L    N+PTEF+WP  DL   V + +EL+EPLIDL GF +GD  ATADAA ++R AC NH
Sbjct: 35  LQKQANMPTEFIWPIGDL---VHNQDELKEPLIDLDGFLKGDERATADAAELVRTACLNH 94

Query: 73  GLFQVTNHGVDPDLIRAAYEQVEMIFKMPLGKKKSVGKKAGRVCGYSGAHADRFSSKLPW 132
           G FQV NHGVD  LI AA+E+++ IFK+PL KK S  +K G V GYSGAHA R+SSKLPW
Sbjct: 95  GFFQVINHGVDIGLIHAAHEEIDKIFKLPLDKKLSTRRKPGDVSGYSGAHAHRYSSKLPW 154

Query: 133 KETFSFEYCSDDSQRLHVLHYFKSFLGQDFEKTGWVYQKYCEEMTRTGLMIMELLAISLG 192
           KETFSF Y  DD     V+ YFKS LG++FE TGWVYQ+YCE M +  L+I ELL ISLG
Sbjct: 155 KETFSFGYHGDDDSEPLVVDYFKSVLGENFEHTGWVYQRYCEAMKKVSLVIFELLGISLG 214

Query: 193 VERLHYRKFFEDGRSIMRCNYYPPCENAALTFGTGPHCDPTSLTILHQDQVGGLEVFANN 252
           V+RLHYRKFFEDG SIMRCN YPPC N++LT GTGPHCDPTSLTILHQDQVGGLEVFANN
Sbjct: 215 VDRLHYRKFFEDGSSIMRCNNYPPCNNSSLTLGTGPHCDPTSLTILHQDQVGGLEVFANN 274

Query: 253 TWLSVKPRPDALVINIGDTFMALSNGAYKSCLHRAVVNRKRVRRSLVFFVCPKDDKVVQP 312
            W +++PRPDALV+NIGDTF ALSNG Y+SCLHRAVVNR+R R+SLVFFV PK++KVV+P
Sbjct: 275 KWQAIRPRPDALVVNIGDTFTALSNGRYQSCLHRAVVNRERERKSLVFFVSPKEEKVVRP 334

Query: 313 PRDLVGREGPRQYPDFTWSDLLEFTQKHYRADVATLQSFVHWLRSS 359
           P+DLV REGPR+YPDFTWSDLLEFTQKHYRADVATLQSF+ WL S+
Sbjct: 335 PQDLVCREGPRKYPDFTWSDLLEFTQKHYRADVATLQSFIQWLLSA 377

BLAST of Cp4.1LG10g03620 vs. TrEMBL
Match: A0A061DRN7_THECC (Gibberellin 20-oxidase, putative OS=Theobroma cacao GN=TCM_001532 PE=3 SV=1)

HSP 1 Score: 522.3 bits (1344), Expect = 4.7e-145
Identity = 248/353 (70.25%), Postives = 282/353 (79.89%), Query Frame = 1

Query: 10  PPTLLHNGNLPTEFVWPHADLAAIVDDPEELQEPLIDLAGFRRGDAAATADAAAMIRVAC 69
           P  L    N+P EFVWPH DL   V   EEL EPLIDL GF +GD  AT+ A  ++R AC
Sbjct: 68  PSKLQKQANIPAEFVWPHVDL---VHTQEELNEPLIDLEGFIKGDDEATSHAVDLVRSAC 127

Query: 70  RNHGLFQVTNHGVDPDLIRAAYEQVEMIFKMPLGKKKSVGKKAGRVCGYSGAHADRFSSK 129
            NHG FQVTNHGVD  LI+AAYE+++ +FKMPL KK S  +K G   G+S AHADRFSSK
Sbjct: 128 LNHGFFQVTNHGVDATLIQAAYEEIDPVFKMPLNKKLSFQRKPGDFSGFSAAHADRFSSK 187

Query: 130 LPWKETFSFEYCSDDSQRLHVLHYFKSFLGQDFEKTGWVYQKYCEEMTRTGLMIMELLAI 189
           LPWKETFSF Y  ++S  + V+ YF+S LG+DFE TGW+YQ YCE+M    L+I ELLA+
Sbjct: 188 LPWKETFSFGYHGNNSDPV-VVDYFRSALGEDFEHTGWIYQNYCEKMRELSLVIFELLAV 247

Query: 190 SLGVERLHYRKFFEDGRSIMRCNYYPPCENAALTFGTGPHCDPTSLTILHQDQVGGLEVF 249
           SLGV+R+HY KFFEDG SIMRCNYYPPC N+ LT GTGPHCDPTSLTILHQDQVGGLEVF
Sbjct: 248 SLGVDRMHYHKFFEDGNSIMRCNYYPPCNNSGLTLGTGPHCDPTSLTILHQDQVGGLEVF 307

Query: 250 ANNTWLSVKPRPDALVINIGDTFMALSNGAYKSCLHRAVVNRKRVRRSLVFFVCPKDDKV 309
           ANN W +V+PRPDALVINIGDTFMAL NG YKSCLHRAVVN +RVRRSLV+FVCPK+DKV
Sbjct: 308 ANNKWQTVRPRPDALVINIGDTFMALCNGRYKSCLHRAVVNSERVRRSLVYFVCPKEDKV 367

Query: 310 VQPPRDLVGREGPRQYPDFTWSDLLEFTQKHYRADVATLQSFVHWLRSSPSTN 363
           V+PP+DLV R+ PR YPDFTWSDLLEFTQKHYRADV TLQSF  WL SS  +N
Sbjct: 368 VRPPQDLVCRDVPRLYPDFTWSDLLEFTQKHYRADVDTLQSFFPWLLSSKRSN 416

BLAST of Cp4.1LG10g03620 vs. TrEMBL
Match: W9QWT0_9ROSA (Gibberellin 20 oxidase 2 OS=Morus notabilis GN=L484_020676 PE=3 SV=1)

HSP 1 Score: 520.0 bits (1338), Expect = 2.3e-144
Identity = 247/344 (71.80%), Postives = 282/344 (81.98%), Query Frame = 1

Query: 18  NLPTEFVWPHADLAAIVDDPEELQEPLIDLAGFRRGDAAATADAAAMIRVACRNHGLFQV 77
           NLP +FVWP+ DL  +    EEL EPL+DL GF RGD  A   AA ++R AC NHG FQ+
Sbjct: 42  NLPNQFVWPNGDL--VHGTQEELNEPLVDLEGFLRGDEFAADHAAELVRTACLNHGFFQM 101

Query: 78  TNHGVDPDLIRAAYEQVEMIFKMPLGKKKSVGKKAGRVCGYSGAHADRFSSKLPWKETFS 137
            NHGVD DLIRAA+E+++ IF +PL KK SV +K G + GYSGAHADR+SSKLPWKETFS
Sbjct: 102 INHGVDLDLIRAAHEEMDTIFGLPLNKKLSVWRKPGSLLGYSGAHADRYSSKLPWKETFS 161

Query: 138 FEYCSDDSQRLH---VLHYFKSFLGQDFEKTGWVYQKYCEEMTRTGLMIMELLAISLGVE 197
           F Y   D        V+ YFKS LG+DF  TGWVYQ+YC+ M    L+IMELLA+SLGV+
Sbjct: 162 FGYRESDDDAYSEPVVVDYFKSALGEDFNCTGWVYQRYCKAMRELSLVIMELLAVSLGVD 221

Query: 198 RLHYRKFFEDGRSIMRCNYYPPCENAALTFGTGPHCDPTSLTILHQDQVGGLEVFANNTW 257
           RL+YRKFFED  SIMRCNYYPPC+N+ LT GTGPH DPTSLTILHQDQVGGLEVFANNTW
Sbjct: 222 RLYYRKFFEDSESIMRCNYYPPCKNSGLTLGTGPHSDPTSLTILHQDQVGGLEVFANNTW 281

Query: 258 LSVKPRPDALVINIGDTFMALSNGAYKSCLHRAVVNRKRVRRSLVFFVCPKDDKVVQPPR 317
            +V+PRP+ALVINIGDTFMALSNG YKSCLHRAVVNR+RVRRSLVFFV P++DKVV+PP+
Sbjct: 282 QAVRPRPEALVINIGDTFMALSNGRYKSCLHRAVVNRERVRRSLVFFVSPREDKVVRPPK 341

Query: 318 DLVGREGPRQYPDFTWSDLLEFTQKHYRADVATLQSFVHWLRSS 359
           DLV REGPR+YPDFTWSDLLEFTQ HYRADVATLQSF+HWL SS
Sbjct: 342 DLVCREGPRKYPDFTWSDLLEFTQNHYRADVATLQSFIHWLLSS 383

BLAST of Cp4.1LG10g03620 vs. TAIR10
Match: AT4G25420.1 (AT4G25420.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 392.9 bits (1008), Expect = 2.2e-109
Identity = 190/346 (54.91%), Postives = 235/346 (67.92%), Query Frame = 1

Query: 10  PPTLLHNGNLPTEFVWPHADLAAIVDDPEELQEPLIDLAGFRRGDAAATADAAAMIRVAC 69
           P  L    N+P +F+WP  +  +I  +  EL  PLIDL      D ++T DA+ +I  AC
Sbjct: 31  PSMLNLQANIPNQFIWPDDEKPSI--NVLELDVPLIDLQNLL-SDPSSTLDASRLISEAC 90

Query: 70  RNHGLFQVTNHGVDPDLIRAAYEQVEMIFKMPLGKKKSVGKKAGRVCGYSGAHADRFSSK 129
           + HG F V NHG+  +LI  A+E     F MPL +K+ V +K+G   GY+ +   RFS+K
Sbjct: 91  KKHGFFLVVNHGISEELISDAHEYTSRFFDMPLSEKQRVLRKSGESVGYASSFTGRFSTK 150

Query: 130 LPWKETFSFEYCSDDSQRLHVLHYFKSFLGQDFEKTGWVYQKYCEEMTRTGLMIMELLAI 189
           LPWKET SF +C D S+   V  YF   LG  F+  G VYQ+YCE M+   L IMELL +
Sbjct: 151 LPWKETLSFRFCDDMSRSKSVQDYFCDALGHGFQPFGKVYQEYCEAMSSLSLKIMELLGL 210

Query: 190 SLGVERLHYRKFFEDGRSIMRCNYYPPCENAALTFGTGPHCDPTSLTILHQDQVGGLEVF 249
           SLGV+R ++R+FFE+  SIMR NYYPPC    LT GTGPHCDPTSLTILHQD V GL+VF
Sbjct: 211 SLGVKRDYFREFFEENDSIMRLNYYPPCIKPDLTLGTGPHCDPTSLTILHQDHVNGLQVF 270

Query: 250 ANNTWLSVKPRPDALVINIGDTFMALSNGAYKSCLHRAVVNRKRVRRSLVFFVCPKDDKV 309
             N W S++P P A V+NIGDTFMALSN  YKSCLHRAVVN +  R+SL FF+CPK D+V
Sbjct: 271 VENQWRSIRPNPKAFVVNIGDTFMALSNDRYKSCLHRAVVNSESERKSLAFFLCPKKDRV 330

Query: 310 VQPPRDLVGREGPRQYPDFTWSDLLEFTQKHYRADVATLQSFVHWL 356
           V PPR+L+     R+YPDFTWS  LEFTQKHYRAD+ TLQ+F  WL
Sbjct: 331 VTPPRELLDSITSRRYPDFTWSMFLEFTQKHYRADMNTLQAFSDWL 373

BLAST of Cp4.1LG10g03620 vs. TAIR10
Match: AT5G51810.1 (AT5G51810.1 gibberellin 20 oxidase 2)

HSP 1 Score: 391.3 bits (1004), Expect = 6.3e-109
Identity = 188/346 (54.34%), Postives = 237/346 (68.50%), Query Frame = 1

Query: 11  PTLLH-NGNLPTEFVWPHADLAAIVDDPEELQEPLIDLAGFRRGDAAATADAAAMIRVAC 70
           P+LL+    +P +F+WP  +  +I  D  EL  P IDL+        +T +A  +I  AC
Sbjct: 33  PSLLNLQSQIPNQFIWPDEEKPSI--DIPELNVPFIDLSS-----QDSTLEAPRVIAEAC 92

Query: 71  RNHGLFQVTNHGVDPDLIRAAYEQVEMIFKMPLGKKKSVGKKAGRVCGYSGAHADRFSSK 130
             HG F V NHGV   LI  A+  +E  F MPL  K+   +K G  CGY+ +   RFS+K
Sbjct: 93  TKHGFFLVVNHGVSESLIADAHRLMESFFDMPLAGKQKAQRKPGESCGYASSFTGRFSTK 152

Query: 131 LPWKETFSFEYCSDDSQRLHVLHYFKSFLGQDFEKTGWVYQKYCEEMTRTGLMIMELLAI 190
           LPWKET SF++ +D+S    V  YF   LGQ+FE+ G VYQ YCE M+   L IMELL +
Sbjct: 153 LPWKETLSFQFSNDNSGSRTVQDYFSDTLGQEFEQFGKVYQDYCEAMSSLSLKIMELLGL 212

Query: 191 SLGVERLHYRKFFEDGRSIMRCNYYPPCENAALTFGTGPHCDPTSLTILHQDQVGGLEVF 250
           SLGV R ++R FFE+  SIMR N+YPPC+   LT GTGPHCDP+SLTILHQD V GL+VF
Sbjct: 213 SLGVNRDYFRGFFEENDSIMRLNHYPPCQTPDLTLGTGPHCDPSSLTILHQDHVNGLQVF 272

Query: 251 ANNTWLSVKPRPDALVINIGDTFMALSNGAYKSCLHRAVVNRKRVRRSLVFFVCPKDDKV 310
            +N W S++P P A V+NIGDTFMALSNG +KSCLHRAVVNR+  R+S+ FF+CPK DKV
Sbjct: 273 VDNQWQSIRPNPKAFVVNIGDTFMALSNGIFKSCLHRAVVNRESARKSMAFFLCPKKDKV 332

Query: 311 VQPPRDLVGREGPRQYPDFTWSDLLEFTQKHYRADVATLQSFVHWL 356
           V+PP D++ +   R+YPDFTWS  LEFTQKHYRADV TL SF +W+
Sbjct: 333 VKPPSDILEKMKTRKYPDFTWSMFLEFTQKHYRADVNTLDSFSNWV 371

BLAST of Cp4.1LG10g03620 vs. TAIR10
Match: AT5G07200.1 (AT5G07200.1 gibberellin 20-oxidase 3)

HSP 1 Score: 381.7 bits (979), Expect = 5.0e-106
Identity = 183/349 (52.44%), Postives = 235/349 (67.34%), Query Frame = 1

Query: 15  HNGNLPTEFVWPHADLAAIVDDPEELQEPLIDLAGFRRGDAAATADAAAMIRVACRNHGL 74
           H+ ++P +FVWP  +  +   D + LQ PLIDLAGF  GD+   ++A  ++  A   HG 
Sbjct: 33  HSHHIPQQFVWPDHEKPST--DVQPLQVPLIDLAGFLSGDSCLASEATRLVSKAATKHGF 92

Query: 75  FQVTNHGVDPDLIRAAYEQVEMIFKMPLGKKKSVGKKAGRVCGYSGAHADRFSSKLPWKE 134
           F +TNHGVD  L+  AY  ++  FK P  +K+   +K G   GY+ +   RFSSKLPWKE
Sbjct: 93  FLITNHGVDESLLSRAYLHMDSFFKAPACEKQKAQRKWGESSGYASSFVGRFSSKLPWKE 152

Query: 135 TFSFEYCSDDSQRLHVLHYFKSF-LGQDFEKTGWVYQKYCEEMTRTGLMIMELLAISLGV 194
           T SF++  ++      +  F S  +G  +E  G VYQ+Y E M    L IMELL +SLGV
Sbjct: 153 TLSFKFSPEEKIHSQTVKDFVSKKMGDGYEDFGKVYQEYAEAMNTLSLKIMELLGMSLGV 212

Query: 195 ERLHYRKFFEDGRSIMRCNYYPPCENAALTFGTGPHCDPTSLTILHQDQVGGLEVFANNT 254
           ER ++++FFED  SI R NYYP C+   L  GTGPHCDPTSLTILHQDQVGGL+VF +N 
Sbjct: 213 ERRYFKEFFEDSDSIFRLNYYPQCKQPELALGTGPHCDPTSLTILHQDQVGGLQVFVDNK 272

Query: 255 WLSVKPRPDALVINIGDTFMALSNGAYKSCLHRAVVNRKRVRRSLVFFVCPKDDKVVQPP 314
           W S+ P P A V+NIGDTFMAL+NG YKSCLHRAVVN +R R++  FF+CPK +KVV+PP
Sbjct: 273 WQSIPPNPHAFVVNIGDTFMALTNGRYKSCLHRAVVNSERERKTFAFFLCPKGEKVVKPP 332

Query: 315 RDLVG--REGPRQYPDFTWSDLLEFTQKHYRADVATLQSFVHWLRSSPS 361
            +LV   + G R+YPDFTWS  LEFTQKHYRAD+ TL  F  WL++  S
Sbjct: 333 EELVNGVKSGERKYPDFTWSMFLEFTQKHYRADMNTLDEFSIWLKNRRS 379

BLAST of Cp4.1LG10g03620 vs. TAIR10
Match: AT1G60980.1 (AT1G60980.1 gibberellin 20-oxidase 4)

HSP 1 Score: 360.5 bits (924), Expect = 1.2e-99
Identity = 174/346 (50.29%), Postives = 225/346 (65.03%), Query Frame = 1

Query: 18  NLPTEFVWPHADLAAIVDDPEELQEPLIDLAGFRRGDAAATADAAAMIRVACRNHGLFQV 77
           ++P EFVWP  +  +   +   LQ P+IDLAGF   D    ++A  ++  A + HG F V
Sbjct: 35  HIPQEFVWPDHEKPS--KNVPILQVPVIDLAGFLSNDPLLVSEAERLVSEAAKKHGFFLV 94

Query: 78  TNHGVDPDLIRAAYEQVEMIFKMPLGKKKSVGKKAGRVCGYSGAHADRFSSKLPWKETFS 137
           TNHGVD  L+  A++ ++  FK P  +K    +K G   GY+ +   RF   LPWKET S
Sbjct: 95  TNHGVDERLLSTAHKLMDTFFKSPNYEKLKAQRKVGETTGYASSFVGRFKENLPWKETLS 154

Query: 138 FEYCSDDSQRLH---VLHYFKSFLGQDFEKTGWVYQKYCEEMTRTGLMIMELLAISLGVE 197
           F +   +    +   V +Y    +G  ++  G VYQ+Y E M+   L IMELL +SLG++
Sbjct: 155 FSFSPTEKSENYSQTVKNYISKTMGDGYKDFGSVYQEYAETMSNLSLKIMELLGMSLGIK 214

Query: 198 RLHYRKFFEDGRSIMRCNYYPPCENAALTFGTGPHCDPTSLTILHQDQVGGLEVFANNTW 257
           R H+R+FFED  SI R NYYP C+   L  GTGPHCDPTSLTIL QDQV GL+VF +N W
Sbjct: 215 REHFREFFEDNESIFRLNYYPKCKQPDLVLGTGPHCDPTSLTILQQDQVSGLQVFVDNQW 274

Query: 258 LSVKPRPDALVINIGDTFMALSNGAYKSCLHRAVVNRKRVRRSLVFFVCPKDDKVVQPPR 317
            S+ P P ALV+NIGDT MAL+NG YKSCLHRAVVN +  R++L FF+CPK DKVV+PP 
Sbjct: 275 QSIPPIPQALVVNIGDTLMALTNGIYKSCLHRAVVNGETTRKTLAFFLCPKVDKVVKPPS 334

Query: 318 DLVGREGPRQYPDFTWSDLLEFTQKHYRADVATLQSFVHWLRSSPS 361
           +L   EG R YPDFTWS  LEFT KHYRAD+ TL+ F +WL++  S
Sbjct: 335 EL---EGERAYPDFTWSMFLEFTMKHYRADMNTLEEFTNWLKNKGS 375

BLAST of Cp4.1LG10g03620 vs. TAIR10
Match: AT1G44090.1 (AT1G44090.1 gibberellin 20-oxidase 5)

HSP 1 Score: 336.3 bits (861), Expect = 2.4e-92
Identity = 168/349 (48.14%), Postives = 221/349 (63.32%), Query Frame = 1

Query: 18  NLPTEFVWPHADLAAIVDDPEELQEPLIDLAGFRRGDAAATADAAAMIRVACRNHGLFQV 77
           N+P EF WP  D+A    D   L  P+IDL+GF  G+ A T  AA  ++ AC  HG F V
Sbjct: 42  NVPAEFFWPEKDVAPSEGD---LDLPIIDLSGFLNGNEAETQLAAKAVKKACMAHGTFLV 101

Query: 78  TNHGVDPDLIRAAYEQVEMIFKMPLGKKKSVGKKAGRVCGYSGAHADRFSSKLPWKETFS 137
            NHG    L   A E   + F +   +K    +  G + GY+  H+ RFSS LPW ET +
Sbjct: 102 VNHGFKSGLAEKALEISSLFFGLSKDEKLRAYRIPGNISGYTAGHSQRFSSNLPWNETLT 161

Query: 138 FEYCSDDSQRLHVLHYF-KSFLGQDFEKTGWVYQKYCEEMTRTGLMIMELLAISLGV-ER 197
             +        HV+  F  S LG   ++ G V+Q++C+ M    + +MELL IS+G+ +R
Sbjct: 162 LAFKKGPP---HVVEDFLTSRLGNHRQEIGQVFQEFCDAMNGLVMDLMELLGISMGLKDR 221

Query: 198 LHYRKFFEDGRSIMRCNYYPPCENAALTFGTGPHCDPTSLTILHQDQVGGLEVFANNTWL 257
            +YR+FFEDG  I RCNYYPPC+      G GPH DPT++T+L QD V GLEVFA  +W 
Sbjct: 222 TYYRRFFEDGSGIFRCNYYPPCKQPEKALGVGPHNDPTAITVLLQDDVVGLEVFAAGSWQ 281

Query: 258 SVKPRPDALVINIGDTFMALSNGAYKSCLHRAVVNRKRVRRSLVFFVCPKDDKVVQPPRD 317
           +V+PRP ALV+N+GDTFMALSNG Y+SC HRAVVN+++VRRSLVFF CP++DK++ PP +
Sbjct: 282 TVRPRPGALVVNVGDTFMALSNGNYRSCYHRAVVNKEKVRRSLVFFSCPREDKIIVPPPE 341

Query: 318 LV-GREGPRQYPDFTWSDLLEFTQKHYRADVATLQSFVHWLRSSPSTNS 364
           LV G E  R+YPDFTW+ L +FTQ  YR D  TL +F  WL S+    S
Sbjct: 342 LVEGEEASRKYPDFTWAQLQKFTQSGYRVDNTTLHNFSSWLVSNSDKKS 384

BLAST of Cp4.1LG10g03620 vs. NCBI nr
Match: gi|659125355|ref|XP_008462644.1| (PREDICTED: gibberellin 20 oxidase 2-like [Cucumis melo])

HSP 1 Score: 607.1 bits (1564), Expect = 2.1e-170
Identity = 291/360 (80.83%), Postives = 315/360 (87.50%), Query Frame = 1

Query: 1   MGSTGTAAIPPTLLHNGNLPTEFVWPHADLAAIVDDPEELQEPLIDLAGFRRGDAAATAD 60
           +G      +P  +     LP EFVWP  +LA   ++ EEL+EPLIDL GFRRG+  ATA+
Sbjct: 7   VGRVAGIILPTMVDKKKKLPREFVWPRGELAG--EEREELKEPLIDLGGFRRGEEEATAE 66

Query: 61  AAAMIRVACRNHGLFQVTNHGVDPDLIRAAYEQVEMIFKMPLGKKKSVGKKAGRVCGYSG 120
           AAAM+RVAC  HG+FQVTNHGV+ +LI+AAYE+ E IFKMPL KK SVGKK GRV GYSG
Sbjct: 67  AAAMVRVACMKHGVFQVTNHGVEEELIKAAYEEGEGIFKMPLVKKISVGKKPGRVSGYSG 126

Query: 121 AHADRFSSKLPWKETFSFEYCSDDS-QRLHVLHYFKSFLGQDFEKTGWVYQKYCEEMTRT 180
           AHADRFSSKLPWKETFSFEY +DDS Q LHVLHYFKS  G DFE TGWVYQ+YCEEMTR 
Sbjct: 127 AHADRFSSKLPWKETFSFEYSNDDSDQPLHVLHYFKSLFGCDFENTGWVYQRYCEEMTRI 186

Query: 181 GLMIMELLAISLGVERLHYRKFFEDGRSIMRCNYYPPCENAALTFGTGPHCDPTSLTILH 240
            LMIMELLAISLGVER HYRKFFEDG+SIMRCNYYPPCENA+LT GTGPHCDPTSLTILH
Sbjct: 187 ALMIMELLAISLGVERYHYRKFFEDGKSIMRCNYYPPCENASLTLGTGPHCDPTSLTILH 246

Query: 241 QDQVGGLEVFANNTWLSVKPRPDALVINIGDTFMALSNGAYKSCLHRAVVNRKRVRRSLV 300
           QDQVGGLEVFANNTWLSVKPRPDALVINIGDTFMALSNGAYKSCLHRAVVNRKR RRSLV
Sbjct: 247 QDQVGGLEVFANNTWLSVKPRPDALVINIGDTFMALSNGAYKSCLHRAVVNRKRERRSLV 306

Query: 301 FFVCPKDDKVVQPPRDLVGREGPRQYPDFTWSDLLEFTQKHYRADVATLQSFVHWLRSSP 360
           FFVCPKDDKVV+PP+DLVGREGPRQYPDFTWS+LLEFTQKHYRADVATLQSFVHWL++ P
Sbjct: 307 FFVCPKDDKVVRPPQDLVGREGPRQYPDFTWSELLEFTQKHYRADVATLQSFVHWLQAKP 364

BLAST of Cp4.1LG10g03620 vs. NCBI nr
Match: gi|778717666|ref|XP_004143402.2| (PREDICTED: gibberellin 20 oxidase 2-like [Cucumis sativus])

HSP 1 Score: 606.3 bits (1562), Expect = 3.5e-170
Identity = 288/359 (80.22%), Postives = 314/359 (87.47%), Query Frame = 1

Query: 1   MGSTGTAAIPPTLLHNGNLPTEFVWPHADLAAIVDDPEELQEPLIDLAGFRRGDAAATAD 60
           +G      +P  +     LP EFVWP  +LA   ++  EL+EPLIDL GFRRG+  ATA+
Sbjct: 7   VGRVAGIILPTMVDKKKKLPREFVWPRGELAG--EERGELKEPLIDLGGFRRGEEEATAE 66

Query: 61  AAAMIRVACRNHGLFQVTNHGVDPDLIRAAYEQVEMIFKMPLGKKKSVGKKAGRVCGYSG 120
           AAAM+R+AC  HG+FQVTNHGV+ +LI+AAYE+ E IFKMPL KK SVGKK GRV GYSG
Sbjct: 67  AAAMVRMACMKHGVFQVTNHGVEEELIKAAYEEGEGIFKMPLVKKISVGKKPGRVSGYSG 126

Query: 121 AHADRFSSKLPWKETFSFEYCSDDSQRLHVLHYFKSFLGQDFEKTGWVYQKYCEEMTRTG 180
           AHADRFSSKLPWKETFSFEY +DDSQ LHVLH+FKS  G DFE TGWVYQ+YCEEMTRT 
Sbjct: 127 AHADRFSSKLPWKETFSFEYSNDDSQPLHVLHHFKSLFGCDFENTGWVYQRYCEEMTRTA 186

Query: 181 LMIMELLAISLGVERLHYRKFFEDGRSIMRCNYYPPCENAALTFGTGPHCDPTSLTILHQ 240
           LMIMELLAISLGVER HYRKFFEDG+SIMRCNYYPPCENA+LT GTGPHCDPTSLTILHQ
Sbjct: 187 LMIMELLAISLGVERYHYRKFFEDGKSIMRCNYYPPCENASLTLGTGPHCDPTSLTILHQ 246

Query: 241 DQVGGLEVFANNTWLSVKPRPDALVINIGDTFMALSNGAYKSCLHRAVVNRKRVRRSLVF 300
           DQVGGLEVFANN WLSVKPRPDALVINIGDTFMALSNGAYKSCLHRAVVNRKR RRSLVF
Sbjct: 247 DQVGGLEVFANNAWLSVKPRPDALVINIGDTFMALSNGAYKSCLHRAVVNRKRERRSLVF 306

Query: 301 FVCPKDDKVVQPPRDLVGREGPRQYPDFTWSDLLEFTQKHYRADVATLQSFVHWLRSSP 360
           FVCPKDDKVV+PP+DLVGREGPRQYPDFTWS+LLEFTQKHYRADVATLQSFVHWL++ P
Sbjct: 307 FVCPKDDKVVRPPQDLVGREGPRQYPDFTWSELLEFTQKHYRADVATLQSFVHWLQAKP 363

BLAST of Cp4.1LG10g03620 vs. NCBI nr
Match: gi|743827037|ref|XP_011022922.1| (PREDICTED: gibberellin 20 oxidase 1-D-like [Populus euphratica])

HSP 1 Score: 533.1 bits (1372), Expect = 3.8e-148
Identity = 252/349 (72.21%), Postives = 285/349 (81.66%), Query Frame = 1

Query: 13  LLHNGNLPTEFVWPHADLAAIVDDPEELQEPLIDLAGFRRGDAAATADAAAMIRVACRNH 72
           L    NLPTEF+WPH DL   V + +EL+EP+IDL GF +GD  ATA AA ++R AC NH
Sbjct: 35  LQKQANLPTEFMWPHGDL---VHNEDELKEPVIDLEGFLKGDEVATAQAAELVRTACLNH 94

Query: 73  GLFQVTNHGVDPDLIRAAYEQVEMIFKMPLGKKKSVGKKAGRVCGYSGAHADRFSSKLPW 132
           G FQVTNHGVD  LI +A+E++  IFK+P+ KK SV +K G V GYSGAHA R+SSKLPW
Sbjct: 95  GFFQVTNHGVDISLIHSAHEEIGKIFKLPIDKKLSVRRKPGDVSGYSGAHAHRYSSKLPW 154

Query: 133 KETFSFEYCSDDSQRLHVLHYFKSFLGQDFEKTGWVYQKYCEEMTRTGLMIMELLAISLG 192
           KETFSF Y  DD     V+ YFKS LG+DFE TGWVYQ YCE M +  L+I ELLAISLG
Sbjct: 155 KETFSFGYHGDDDSVPLVVDYFKSALGKDFEHTGWVYQSYCEAMKKVSLVIFELLAISLG 214

Query: 193 VERLHYRKFFEDGRSIMRCNYYPPCENAALTFGTGPHCDPTSLTILHQDQVGGLEVFANN 252
           V+RLHYRKFFEDG SIMRCNYYPPC N+ LT GTGPHCDPTSLTILHQDQVGGL+VF+NN
Sbjct: 215 VDRLHYRKFFEDGSSIMRCNYYPPCNNSTLTLGTGPHCDPTSLTILHQDQVGGLQVFSNN 274

Query: 253 TWLSVKPRPDALVINIGDTFMALSNGAYKSCLHRAVVNRKRVRRSLVFFVCPKDDKVVQP 312
            WL+++PRPDALV+NIGDTFMALSNG YKSCLHRAVVNR   RRSLVFFV PK++KVV+P
Sbjct: 275 KWLAIRPRPDALVVNIGDTFMALSNGRYKSCLHRAVVNRDSGRRSLVFFVSPKEEKVVRP 334

Query: 313 PRDLVGREGPRQYPDFTWSDLLEFTQKHYRADVATLQSFVHWLRSSPST 362
           PRDLV REG R YPDFTWSDLLEFTQKHYRADVATLQSF+ WL SS S+
Sbjct: 335 PRDLVSREGQRIYPDFTWSDLLEFTQKHYRADVATLQSFIQWLLSSKSS 380

BLAST of Cp4.1LG10g03620 vs. NCBI nr
Match: gi|224096043|ref|XP_002310523.1| (gibberellin 20-oxidase family protein [Populus trichocarpa])

HSP 1 Score: 531.2 bits (1367), Expect = 1.4e-147
Identity = 252/350 (72.00%), Postives = 286/350 (81.71%), Query Frame = 1

Query: 13  LLHNGNLPTEFVWPHADLAAIVDDPEELQEPLIDLAGFRRGDAAATADAAAMIRVACRNH 72
           L    +LPTEF+WPH D   +V + +EL+EP+IDL GF +GD  ATA AA ++R AC NH
Sbjct: 31  LQKQASLPTEFMWPHGD---LVHNEDELKEPMIDLEGFLKGDEVATAQAAELVRTACLNH 90

Query: 73  GLFQVTNHGVDPDLIRAAYEQVEMIFKMPLGKKKSVGKKAGRVCGYSGAHADRFSSKLPW 132
           G FQVTNHGVD  LI +A+E++  IFK+PL KK SV +K G V GYSGAHA R+SSKLPW
Sbjct: 91  GFFQVTNHGVDISLIHSAHEEIGKIFKLPLDKKLSVRRKPGDVSGYSGAHAHRYSSKLPW 150

Query: 133 KETFSFEYCSDDSQRLHVLHYFKSFLGQDFEKTGWVYQKYCEEMTRTGLMIMELLAISLG 192
           KETFSF Y  DD     V+ YFKS LG+DFE TGWVYQ YCE M +  L+I ELLAISLG
Sbjct: 151 KETFSFGYHGDDDSVPLVVDYFKSVLGKDFEHTGWVYQSYCEAMKKVSLVIFELLAISLG 210

Query: 193 VERLHYRKFFEDGRSIMRCNYYPPCENAALTFGTGPHCDPTSLTILHQDQVGGLEVFANN 252
           V+RLHYRKFFEDG SIMRCNYYPPC N+ LT GTGPHCDPTSLTILHQDQVGGL+VF+NN
Sbjct: 211 VDRLHYRKFFEDGSSIMRCNYYPPCNNSTLTLGTGPHCDPTSLTILHQDQVGGLQVFSNN 270

Query: 253 TWLSVKPRPDALVINIGDTFMALSNGAYKSCLHRAVVNRKRVRRSLVFFVCPKDDKVVQP 312
            WL+++PRPDALV+NIGDTFMALSNG YKSCLHRAVVNR   RRSLVFFV PK++KVV+P
Sbjct: 271 KWLAIRPRPDALVVNIGDTFMALSNGRYKSCLHRAVVNRDSERRSLVFFVSPKEEKVVRP 330

Query: 313 PRDLVGREGPRQYPDFTWSDLLEFTQKHYRADVATLQSFVHWLRSS-PST 362
           P+DLV REG R YPDFTWSDLLEFTQKHYRADVATLQSF+ WL SS PST
Sbjct: 331 PQDLVSREGQRIYPDFTWSDLLEFTQKHYRADVATLQSFIQWLSSSKPST 377

BLAST of Cp4.1LG10g03620 vs. NCBI nr
Match: gi|224083474|ref|XP_002307041.1| (gibberellin 20-oxidase family protein [Populus trichocarpa])

HSP 1 Score: 524.6 bits (1350), Expect = 1.4e-145
Identity = 246/346 (71.10%), Postives = 284/346 (82.08%), Query Frame = 1

Query: 13  LLHNGNLPTEFVWPHADLAAIVDDPEELQEPLIDLAGFRRGDAAATADAAAMIRVACRNH 72
           L    N+PTEF+WP  DL   V + +EL+EPLIDL GF +GD  ATADAA ++R AC NH
Sbjct: 35  LQKQANMPTEFIWPIGDL---VHNQDELKEPLIDLDGFLKGDERATADAAELVRTACLNH 94

Query: 73  GLFQVTNHGVDPDLIRAAYEQVEMIFKMPLGKKKSVGKKAGRVCGYSGAHADRFSSKLPW 132
           G FQV NHGVD  LI AA+E+++ IFK+PL KK S  +K G V GYSGAHA R+SSKLPW
Sbjct: 95  GFFQVINHGVDIGLIHAAHEEIDKIFKLPLDKKLSTRRKPGDVSGYSGAHAHRYSSKLPW 154

Query: 133 KETFSFEYCSDDSQRLHVLHYFKSFLGQDFEKTGWVYQKYCEEMTRTGLMIMELLAISLG 192
           KETFSF Y  DD     V+ YFKS LG++FE TGWVYQ+YCE M +  L+I ELL ISLG
Sbjct: 155 KETFSFGYHGDDDSEPLVVDYFKSVLGENFEHTGWVYQRYCEAMKKVSLVIFELLGISLG 214

Query: 193 VERLHYRKFFEDGRSIMRCNYYPPCENAALTFGTGPHCDPTSLTILHQDQVGGLEVFANN 252
           V+RLHYRKFFEDG SIMRCN YPPC N++LT GTGPHCDPTSLTILHQDQVGGLEVFANN
Sbjct: 215 VDRLHYRKFFEDGSSIMRCNNYPPCNNSSLTLGTGPHCDPTSLTILHQDQVGGLEVFANN 274

Query: 253 TWLSVKPRPDALVINIGDTFMALSNGAYKSCLHRAVVNRKRVRRSLVFFVCPKDDKVVQP 312
            W +++PRPDALV+NIGDTF ALSNG Y+SCLHRAVVNR+R R+SLVFFV PK++KVV+P
Sbjct: 275 KWQAIRPRPDALVVNIGDTFTALSNGRYQSCLHRAVVNRERERKSLVFFVSPKEEKVVRP 334

Query: 313 PRDLVGREGPRQYPDFTWSDLLEFTQKHYRADVATLQSFVHWLRSS 359
           P+DLV REGPR+YPDFTWSDLLEFTQKHYRADVATLQSF+ WL S+
Sbjct: 335 PQDLVCREGPRKYPDFTWSDLLEFTQKHYRADVATLQSFIQWLLSA 377