Cla008413 (gene) Watermelon (97103) v1

NameCla008413
Typegene
OrganismCitrullus. lanatus (Watermelon (97103) v1)
DescriptionGibberellin 20-oxidase (AHRD V1 ***- B9H4B0_POPTR); contains Interpro domain(s) IPR005123 Oxoglutarate and iron-dependent oxygenase
LocationChr1 : 9551599 .. 9554494 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAATCCACCGGCACGACGGCGGCCGTAATTCTCCCGACGATGTTGGACAACAAGAAGAATCTTCCACGGGAATTTGTGTGGCCACGTGGCGAGTTAGCAGCGGAGAGTGCGGGGGAGCGAGAGGAGCTGAAAGAGCCGTTGATTGACTTAGGGGGATTCCGGCGAGGGGAGGCGGAGGCGACGGCGGAAGCGGCGGCGATGGTGCGAGTGGCGTGTAGGAAACACGGGCTGTTTCAGGTGACGAATCATGGAGTGACGGCGGAGCTGATAAAGGCGGCGTATGAGGAAGGGGAAGCGATATTCAAGATGCCATTAGTGAAGAAAATTAGTGTGGGGAAAAAGCCAGGGAGAGTGTCGGGATATTCAGGCGCTCATGCAGACAGATTCTCGTCAAAGCTTCCATGGAAAGAGACATTCTCTTTTGAGTATTCAAATGATGATTCTCAACGACTTCATGTTCTTCATTACTTTAAATCCTTGTTCGGACACGGCTTCGAGAATACTGGGTCAGTCTTCTCTCATTACAATTTTTTTTTTTTTTTTTTTAAATTTAATTTAATTTATAAAGAAAAAAAAAACCAGCGAAGATCCAAACAGTAGATTTCTAGTTATTAACATATACTATATGTTTGATTTTCTGTTGTGATGTTTAGTGGATTTTTAATCTTTCATACTTCTTTACCGTTAGTTCATCTATAATAAGTACATATAAGTACGGAGGTATATGTTTTATGATTTTTTTTTTATTGTGTCGTGGAGTTTAATAAGTCTTTCAACTTTCATAGTTATTTACAGTTAGTTTATCTAATTTCTTAAAAACGGTAATACATAATAAAATACAAGTACTAAACTATTAAAGTTGACACTAGAGACTTATTAAATAGACAATGGAGGTTTACGTCTGATTTGATAATTATTTTATTAAGAAAACATTTAAATTTATAATCAAATCTCAAAAAATAAATAAATAAATAATGTTTTTAAATACTACATATGTTTCTTTCGGTTTTCAAAATTTTACTTTAAAACATTTTCAACAAAGTAGTGTGTTTTTAAAAACTGTTGGAAGTACATGAAGAAGTAAAAATGAATAATAGACCCATACAAGAGAGAAAGGTAAGCTGAGACATGTTAGTTCAAAAGGATAATATCTAGGGAGCGGGTCGGAGTTTAAGGAAGGAGTATAGTTGTTTAGTCATGGGTCCACAAGGTAAGACGTTAATAAAACAAGAAATTGATGCTTTTTTCGTTGTAGGGTCTACAAATTTTTTGGGCTAAAAACCCTCTTGGATGTTATTATCAATGGGGGCTACAACCATTTTTGACCACAACCGAAAAGTTTTTGAGAATTAAAGAAAAATAAGTTTGTGACGTTTGACGTTGTACTCTAGATGGTTGCAGTTGAACTAGCAGTCAAGTGTTTACAAGCAATTAACATTAGTTGAATTGAAAATGAAGGTATAAGTATAATTTATGGTTTGTGTTTGGTTTTTGATTATGAAGATGGGTATACCAAAGATACTGTGAGGAAATGACAAGAACAGCACTAATGATAATGGAGCTTCTAGCAATAAGTTTGGGAGTAGAGAGATTCCATTACAGGAAGTTCTTTGAAGATGGAAGATCAATAATGAGATGCAATTACTATCCACCATGTGAAAATGCAGGCCTGACCTTAGGCACAGGCCCTCACTGTGACCCAACTTCACTCACAATTCTCCATCAAGATCAAGTTGGAGGCCTTGAAGTTTTTGCTAACAACAGATGGCTCTCTGTCAAACCTAGACCAGATGCTTTGGTTATTAACATTGGAGACACTTTTATGGTACAAAACTTACTCTTACCTTCTTTTTCTATTATTGTTACTGTATTCTATATGTTTCAAAACGACACTGTGACATACGCCCGGTTGGGGTCATTAGCGTTTCAATTGGAGTTAAGAGTTAATTTGGAATGACTTTCTTTTTTGAGTACTTGGAATGATTTGAACGTCTAATCTTTTGGTCGAACTGAAGTATGTTTATGTTAGGAAAAGTACTCATAAAGACATTCCATCTAGGGGGTTAAAGTTAAAAAATTATTGTAAGAGTATGTTTGGAATGACTTTCTTCTCTGGGCAAATTGCAAAATCCATCTTTGACGTACGGTACCGATGGTACTGCAAATACACCCTCAAATTTTAAATTGTAAAAATTGGGCCCTCAAACTTCTAGAAATGTTAAAATTGGTCCCTCAAACTTATAATAATTATAGAAATTGGACCTCCAAATGATAAAAATTGAACACCCAAACTTGAACAATTGTTACAATTTCTACAGATATGTAAGTTTGAGAGTCCAGTTTTAACGCTTGTATAAAGTACTTGCAAGTTTGAGGATATAATTGCAACTACCAAAATACTTTAGGGGTAGTTTCTGCAATTTACTCTTCTTAAATACTAGGAGGGATATGAACCTTTGATCTCTTGGTCGAGATGAACTATGTGTATGTTGGAGAAGTATTTAAAAATTCATTCTAAATCGATTGGTAAAGTTTTTAAAGTTTAAAAAACTATTGTAAGTAGTTGTATTACATTGTTGTAGTCTCTTCGAATAATGAAAAAGCAATGCAGGCACTCTCGAACGGAGCGTACAAGAGCTGCCTGCATAGAGCAGTGGTGAATAGGAAGAGAGAGAGAAGGTCATTGGTGTTCTTTGTGTGCCCAAAAGATGATAAAGTGGTGAGACCCCCACAAGATTTGGTGGGCAGAGAAAGGCCAAGACAGTACCCTGATTTCACATGGTCAGATCTTTTGGAGTTTACACAAAAACATTACAGAGCTGATGTTGCTACGCTCCAAAGCTTTGTTCATTGGCTTCAAGCTAAGCCTCACCCTCCTCCAATTCCATTTTAA

mRNA sequence

ATGGAATCCACCGGCACGACGGCGGCCGTAATTCTCCCGACGATGTTGGACAACAAGAAGAATCTTCCACGGGAATTTGTGTGGCCACGTGGCGAGTTAGCAGCGGAGAGTGCGGGGGAGCGAGAGGAGCTGAAAGAGCCGTTGATTGACTTAGGGGGATTCCGGCGAGGGGAGGCGGAGGCGACGGCGGAAGCGGCGGCGATGGTGCGAGTGGCGTGTAGGAAACACGGGCTGTTTCAGGTGACGAATCATGGAGTGACGGCGGAGCTGATAAAGGCGGCGTATGAGGAAGGGGAAGCGATATTCAAGATGCCATTAGTGAAGAAAATTAGTGTGGGGAAAAAGCCAGGGAGAGTGTCGGGATATTCAGGCGCTCATGCAGACAGATTCTCGTCAAAGCTTCCATGGAAAGAGACATTCTCTTTTGAGTATTCAAATGATGATTCTCAACGACTTCATGTTCTTCATTACTTTAAATCCTTGTTCGGACACGGCTTCGAGAATACTGGATGGGTATACCAAAGATACTGTGAGGAAATGACAAGAACAGCACTAATGATAATGGAGCTTCTAGCAATAAGTTTGGGAGTAGAGAGATTCCATTACAGGAAGTTCTTTGAAGATGGAAGATCAATAATGAGATGCAATTACTATCCACCATGTGAAAATGCAGGCCTGACCTTAGGCACAGGCCCTCACTGTGACCCAACTTCACTCACAATTCTCCATCAAGATCAAGTTGGAGGCCTTGAAGTTTTTGCTAACAACAGATGGCTCTCTGTCAAACCTAGACCAGATGCTTTGGTTATTAACATTGGAGACACTTTTATGGCACTCTCGAACGGAGCGTACAAGAGCTGCCTGCATAGAGCAGTGGTGAATAGGAAGAGAGAGAGAAGGTCATTGGTGTTCTTTGTGTGCCCAAAAGATGATAAAGTGGTGAGACCCCCACAAGATTTGGTGGGCAGAGAAAGGCCAAGACAGTACCCTGATTTCACATGGTCAGATCTTTTGGAGTTTACACAAAAACATTACAGAGCTGATGTTGCTACGCTCCAAAGCTTTGTTCATTGGCTTCAAGCTAAGCCTCACCCTCCTCCAATTCCATTTTAA

Coding sequence (CDS)

ATGGAATCCACCGGCACGACGGCGGCCGTAATTCTCCCGACGATGTTGGACAACAAGAAGAATCTTCCACGGGAATTTGTGTGGCCACGTGGCGAGTTAGCAGCGGAGAGTGCGGGGGAGCGAGAGGAGCTGAAAGAGCCGTTGATTGACTTAGGGGGATTCCGGCGAGGGGAGGCGGAGGCGACGGCGGAAGCGGCGGCGATGGTGCGAGTGGCGTGTAGGAAACACGGGCTGTTTCAGGTGACGAATCATGGAGTGACGGCGGAGCTGATAAAGGCGGCGTATGAGGAAGGGGAAGCGATATTCAAGATGCCATTAGTGAAGAAAATTAGTGTGGGGAAAAAGCCAGGGAGAGTGTCGGGATATTCAGGCGCTCATGCAGACAGATTCTCGTCAAAGCTTCCATGGAAAGAGACATTCTCTTTTGAGTATTCAAATGATGATTCTCAACGACTTCATGTTCTTCATTACTTTAAATCCTTGTTCGGACACGGCTTCGAGAATACTGGATGGGTATACCAAAGATACTGTGAGGAAATGACAAGAACAGCACTAATGATAATGGAGCTTCTAGCAATAAGTTTGGGAGTAGAGAGATTCCATTACAGGAAGTTCTTTGAAGATGGAAGATCAATAATGAGATGCAATTACTATCCACCATGTGAAAATGCAGGCCTGACCTTAGGCACAGGCCCTCACTGTGACCCAACTTCACTCACAATTCTCCATCAAGATCAAGTTGGAGGCCTTGAAGTTTTTGCTAACAACAGATGGCTCTCTGTCAAACCTAGACCAGATGCTTTGGTTATTAACATTGGAGACACTTTTATGGCACTCTCGAACGGAGCGTACAAGAGCTGCCTGCATAGAGCAGTGGTGAATAGGAAGAGAGAGAGAAGGTCATTGGTGTTCTTTGTGTGCCCAAAAGATGATAAAGTGGTGAGACCCCCACAAGATTTGGTGGGCAGAGAAAGGCCAAGACAGTACCCTGATTTCACATGGTCAGATCTTTTGGAGTTTACACAAAAACATTACAGAGCTGATGTTGCTACGCTCCAAAGCTTTGTTCATTGGCTTCAAGCTAAGCCTCACCCTCCTCCAATTCCATTTTAA

Protein sequence

MESTGTTAAVILPTMLDNKKNLPREFVWPRGELAAESAGEREELKEPLIDLGGFRRGEAEATAEAAAMVRVACRKHGLFQVTNHGVTAELIKAAYEEGEAIFKMPLVKKISVGKKPGRVSGYSGAHADRFSSKLPWKETFSFEYSNDDSQRLHVLHYFKSLFGHGFENTGWVYQRYCEEMTRTALMIMELLAISLGVERFHYRKFFEDGRSIMRCNYYPPCENAGLTLGTGPHCDPTSLTILHQDQVGGLEVFANNRWLSVKPRPDALVINIGDTFMALSNGAYKSCLHRAVVNRKRERRSLVFFVCPKDDKVVRPPQDLVGRERPRQYPDFTWSDLLEFTQKHYRADVATLQSFVHWLQAKPHPPPIPF
BLAST of Cla008413 vs. Swiss-Prot
Match: GAOX2_ORYSJ (Gibberellin 20 oxidase 2 OS=Oryza sativa subsp. japonica GN=20ox2 PE=1 SV=1)

HSP 1 Score: 398.3 bits (1022), Expect = 9.3e-110
Identity = 199/352 (56.53%), Postives = 237/352 (67.33%), Query Frame = 1

Query: 8   AAVILPTMLDNKKNLPREFVWPRGELAAESAGEREELKEPLIDLGGFRRGEAEATAEAAA 67
           AA +    ++ K  +P  FVWP G+    SA E   L  P++D+G  R G+AE    AAA
Sbjct: 30  AAAVCDLRMEPK--IPEPFVWPNGDARPASAAE---LDMPVVDVGVLRDGDAEGLRRAAA 89

Query: 68  MVRVACRKHGLFQVTNHGVTAELIKAAYEEGEAIFKMPLVKKISVGKKPGRVSGYSGAHA 127
            V  AC  HG FQV+ HGV A L +AA +     F++PL +K    + PG VSGY+ AHA
Sbjct: 90  QVAAACATHGFFQVSEHGVDAALARAALDGASDFFRLPLAEKRRARRVPGTVSGYTSAHA 149

Query: 128 DRFSSKLPWKETFSFEYSNDDSQRLHVLHYFKSLFGHGFENTGWVYQRYCEEMTRTALMI 187
           DRF+SKLPWKET SF + +D +    V  YF S  G  F   G VYQ+YCEEM   +L I
Sbjct: 150 DRFASKLPWKETLSFGF-HDRAAAPVVADYFSSTLGPDFAPMGRVYQKYCEEMKELSLTI 209

Query: 188 MELLAISLGVERFHYRKFFEDGRSIMRCNYYPPCENAGLTLGTGPHCDPTSLTILHQDQV 247
           MELL +SLGVER +YR+FF D  SIMRCNYYPPC     TLGTGPHCDPT+LTIL QD V
Sbjct: 210 MELLELSLGVERGYYREFFADSSSIMRCNYYPPCPEPERTLGTGPHCDPTALTILLQDDV 269

Query: 248 GGLEVFANNRWLSVKPRPDALVINIGDTFMALSNGAYKSCLHRAVVNRKRERRSLVFFVC 307
           GGLEV  +  W  V P P A+VINIGDTFMALSNG YKSCLHRAVVN++RERRSL FF+C
Sbjct: 270 GGLEVLVDGEWRPVSPVPGAMVINIGDTFMALSNGRYKSCLHRAVVNQRRERRSLAFFLC 329

Query: 308 PKDDKVVRPPQDLVGRERPRQYPDFTWSDLLEFTQKHYRADVATLQSFVHWL 360
           P++D+VVRPP        P+ YPDFTW+DL+ FTQ+HYRAD  TL +F  WL
Sbjct: 330 PREDRVVRPPPSAA---TPQHYPDFTWADLMRFTQRHYRADTRTLDAFTRWL 372

BLAST of Cla008413 vs. Swiss-Prot
Match: GAOX2_ORYSI (Gibberellin 20 oxidase 2 OS=Oryza sativa subsp. indica GN=20ox2 PE=1 SV=1)

HSP 1 Score: 398.3 bits (1022), Expect = 9.3e-110
Identity = 199/352 (56.53%), Postives = 237/352 (67.33%), Query Frame = 1

Query: 8   AAVILPTMLDNKKNLPREFVWPRGELAAESAGEREELKEPLIDLGGFRRGEAEATAEAAA 67
           AA +    ++ K  +P  FVWP G+    SA E   L  P++D+G  R G+AE    AAA
Sbjct: 30  AAAVCDLRMEPK--IPEPFVWPNGDARPASAAE---LDMPVVDVGVLRDGDAEGLRRAAA 89

Query: 68  MVRVACRKHGLFQVTNHGVTAELIKAAYEEGEAIFKMPLVKKISVGKKPGRVSGYSGAHA 127
            V  AC  HG FQV+ HGV A L +AA +     F++PL +K    + PG VSGY+ AHA
Sbjct: 90  QVAAACATHGFFQVSEHGVDAALARAALDGASDFFRLPLAEKRRARRVPGTVSGYTSAHA 149

Query: 128 DRFSSKLPWKETFSFEYSNDDSQRLHVLHYFKSLFGHGFENTGWVYQRYCEEMTRTALMI 187
           DRF+SKLPWKET SF + +D +    V  YF S  G  F   G VYQ+YCEEM   +L I
Sbjct: 150 DRFASKLPWKETLSFGF-HDRAAAPVVADYFSSTLGPDFAPMGRVYQKYCEEMKELSLTI 209

Query: 188 MELLAISLGVERFHYRKFFEDGRSIMRCNYYPPCENAGLTLGTGPHCDPTSLTILHQDQV 247
           MELL +SLGVER +YR+FF D  SIMRCNYYPPC     TLGTGPHCDPT+LTIL QD V
Sbjct: 210 MELLELSLGVERGYYREFFADSSSIMRCNYYPPCPEPERTLGTGPHCDPTALTILLQDDV 269

Query: 248 GGLEVFANNRWLSVKPRPDALVINIGDTFMALSNGAYKSCLHRAVVNRKRERRSLVFFVC 307
           GGLEV  +  W  V P P A+VINIGDTFMALSNG YKSCLHRAVVN++RERRSL FF+C
Sbjct: 270 GGLEVLVDGEWRPVSPVPGAMVINIGDTFMALSNGRYKSCLHRAVVNQRRERRSLAFFLC 329

Query: 308 PKDDKVVRPPQDLVGRERPRQYPDFTWSDLLEFTQKHYRADVATLQSFVHWL 360
           P++D+VVRPP        P+ YPDFTW+DL+ FTQ+HYRAD  TL +F  WL
Sbjct: 330 PREDRVVRPPPSAA---TPQHYPDFTWADLMRFTQRHYRADTRTLDAFTRWL 372

BLAST of Cla008413 vs. Swiss-Prot
Match: GAOX2_ARATH (Gibberellin 20 oxidase 2 OS=Arabidopsis thaliana GN=GA20OX2 PE=2 SV=1)

HSP 1 Score: 393.7 bits (1010), Expect = 2.3e-108
Identity = 191/365 (52.33%), Postives = 245/365 (67.12%), Query Frame = 1

Query: 1   MESTGTTAAVILPTMLDNKKNLPREFVWPRGELAAESAGEREELKEPLIDLGGFRRGEAE 60
           +E   T+  +  P++L+ +  +P +F+WP  E   + + +  EL  P IDL        +
Sbjct: 21  LEKDQTSPLIFNPSLLNLQSQIPNQFIWPDEE---KPSIDIPELNVPFIDLSS-----QD 80

Query: 61  ATAEAAAMVRVACRKHGLFQVTNHGVTAELIKAAYEEGEAIFKMPLVKKISVGKKPGRVS 120
           +T EA  ++  AC KHG F V NHGV+  LI  A+   E+ F MPL  K    +KPG   
Sbjct: 81  STLEAPRVIAEACTKHGFFLVVNHGVSESLIADAHRLMESFFDMPLAGKQKAQRKPGESC 140

Query: 121 GYSGAHADRFSSKLPWKETFSFEYSNDDSQRLHVLHYFKSLFGHGFENTGWVYQRYCEEM 180
           GY+ +   RFS+KLPWKET SF++SND+S    V  YF    G  FE  G VYQ YCE M
Sbjct: 141 GYASSFTGRFSTKLPWKETLSFQFSNDNSGSRTVQDYFSDTLGQEFEQFGKVYQDYCEAM 200

Query: 181 TRTALMIMELLAISLGVERFHYRKFFEDGRSIMRCNYYPPCENAGLTLGTGPHCDPTSLT 240
           +  +L IMELL +SLGV R ++R FFE+  SIMR N+YPPC+   LTLGTGPHCDP+SLT
Sbjct: 201 SSLSLKIMELLGLSLGVNRDYFRGFFEENDSIMRLNHYPPCQTPDLTLGTGPHCDPSSLT 260

Query: 241 ILHQDQVGGLEVFANNRWLSVKPRPDALVINIGDTFMALSNGAYKSCLHRAVVNRKRERR 300
           ILHQD V GL+VF +N+W S++P P A V+NIGDTFMALSNG +KSCLHRAVVNR+  R+
Sbjct: 261 ILHQDHVNGLQVFVDNQWQSIRPNPKAFVVNIGDTFMALSNGIFKSCLHRAVVNRESARK 320

Query: 301 SLVFFVCPKDDKVVRPPQDLVGRERPRQYPDFTWSDLLEFTQKHYRADVATLQSFVHWLQ 360
           S+ FF+CPK DKVV+PP D++ + + R+YPDFTWS  LEFTQKHYRADV TL SF +W+ 
Sbjct: 321 SMAFFLCPKKDKVVKPPSDILEKMKTRKYPDFTWSMFLEFTQKHYRADVNTLDSFSNWVI 377

Query: 361 AKPHP 366
              +P
Sbjct: 381 TNNNP 377

BLAST of Cla008413 vs. Swiss-Prot
Match: GAOX1_ARATH (Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana GN=GA20OX1 PE=2 SV=2)

HSP 1 Score: 391.3 bits (1004), Expect = 1.1e-107
Identity = 192/353 (54.39%), Postives = 241/353 (68.27%), Query Frame = 1

Query: 7   TAAVILPTMLDNKKNLPREFVWPRGELAAESAGEREELKEPLIDLGGFRRGEAEATAEAA 66
           T  +  P+ML+ + N+P +F+WP  E  + +     EL  PLIDL      +  +T +A+
Sbjct: 25  TPLIFNPSMLNLQANIPNQFIWPDDEKPSINV---LELDVPLIDLQNLL-SDPSSTLDAS 84

Query: 67  AMVRVACRKHGLFQVTNHGVTAELIKAAYEEGEAIFKMPLVKKISVGKKPGRVSGYSGAH 126
            ++  AC+KHG F V NHG++ ELI  A+E     F MPL +K  V +K G   GY+ + 
Sbjct: 85  RLISEACKKHGFFLVVNHGISEELISDAHEYTSRFFDMPLSEKQRVLRKSGESVGYASSF 144

Query: 127 ADRFSSKLPWKETFSFEYSNDDSQRLHVLHYFKSLFGHGFENTGWVYQRYCEEMTRTALM 186
             RFS+KLPWKET SF + +D S+   V  YF    GHGF+  G VYQ YCE M+  +L 
Sbjct: 145 TGRFSTKLPWKETLSFRFCDDMSRSKSVQDYFCDALGHGFQPFGKVYQEYCEAMSSLSLK 204

Query: 187 IMELLAISLGVERFHYRKFFEDGRSIMRCNYYPPCENAGLTLGTGPHCDPTSLTILHQDQ 246
           IMELL +SLGV+R ++R+FFE+  SIMR NYYPPC    LTLGTGPHCDPTSLTILHQD 
Sbjct: 205 IMELLGLSLGVKRDYFREFFEENDSIMRLNYYPPCIKPDLTLGTGPHCDPTSLTILHQDH 264

Query: 247 VGGLEVFANNRWLSVKPRPDALVINIGDTFMALSNGAYKSCLHRAVVNRKRERRSLVFFV 306
           V GL+VF  N+W S++P P A V+NIGDTFMALSN  YKSCLHRAVVN + ER+SL FF+
Sbjct: 265 VNGLQVFVENQWRSIRPNPKAFVVNIGDTFMALSNDRYKSCLHRAVVNSESERKSLAFFL 324

Query: 307 CPKDDKVVRPPQDLVGRERPRQYPDFTWSDLLEFTQKHYRADVATLQSFVHWL 360
           CPK D+VV PP++L+     R+YPDFTWS  LEFTQKHYRAD+ TLQ+F  WL
Sbjct: 325 CPKKDRVVTPPRELLDSITSRRYPDFTWSMFLEFTQKHYRADMNTLQAFSDWL 373

BLAST of Cla008413 vs. Swiss-Prot
Match: GAO1B_WHEAT (Gibberellin 20 oxidase 1-B OS=Triticum aestivum GN=GA20ox1B PE=2 SV=1)

HSP 1 Score: 389.4 bits (999), Expect = 4.3e-107
Identity = 192/345 (55.65%), Postives = 228/345 (66.09%), Query Frame = 1

Query: 15  MLDNKKNLPREFVWPRGELAAESAGEREELKEPLIDLGGFRRGEAEATAEAAAMVRVACR 74
           +L  + ++P +F+WP GE     A E  EL  PLID+GG   G+  ATAE   +V  AC 
Sbjct: 10  VLSGRADIPSQFIWPEGESPTPDAAE--ELHVPLIDIGGMLSGDPRATAEVTRLVGEACE 69

Query: 75  KHGLFQVTNHGVTAELIKAAYEEGEAIFKMPLVKKISVGKKPGRVSGYSGAHADRFSSKL 134
           +HG FQV NHG+ AEL+  A+   +A F MPL +K    ++PG   GY+ +   RF+SKL
Sbjct: 70  RHGFFQVVNHGIDAELLADAHRCVDAFFTMPLPEKQRALRRPGESCGYASSFTGRFASKL 129

Query: 135 PWKETFSFEYSNDDSQRLHVLHYFKSLFGHGFENTGWVYQRYCEEMTRTALMIMELLAIS 194
           PWKET SF     D     V+ Y  +  G      G VY RYC EM+R +L IME+L  S
Sbjct: 130 PWKETLSFRSCPSDPAL--VVDYIVATLGEDHRRLGEVYARYCSEMSRLSLEIMEVLGES 189

Query: 195 LGVERFHYRKFFEDGRSIMRCNYYPPCENAGLTLGTGPHCDPTSLTILHQDQVGGLEVFA 254
           LGV R HYR+FFE   SIMR NYYPPC+    TLGTGPHCDPTSLTILHQD VGGL+V  
Sbjct: 190 LGVGRAHYRRFFEGNDSIMRLNYYPPCQRPMETLGTGPHCDPTSLTILHQDNVGGLQVHT 249

Query: 255 NNRWLSVKPRPDALVINIGDTFMALSNGAYKSCLHRAVVNRKRERRSLVFFVCPKDDKVV 314
             RW S++PR DA V+NIGDTFMALSNG YKSCLHRAVVN K  R+SL FF+CP+ DKVV
Sbjct: 250 EGRWRSIRPRADAFVVNIGDTFMALSNGRYKSCLHRAVVNSKVPRKSLAFFLCPEMDKVV 309

Query: 315 RPPQDLVGRERPRQYPDFTWSDLLEFTQKHYRADVATLQSFVHWL 360
            PP  LV    PR YPDFTW  LL+FTQKHYRAD+ TL+ F  W+
Sbjct: 310 APPGTLVDAANPRAYPDFTWRSLLDFTQKHYRADMKTLEVFSSWI 350

BLAST of Cla008413 vs. TrEMBL
Match: S0BDZ8_CUCSA (GA 20-oxidase OS=Cucumis sativus GN=GA20ox2 PE=2 SV=1)

HSP 1 Score: 696.4 bits (1796), Expect = 1.8e-197
Identity = 340/373 (91.15%), Postives = 348/373 (93.30%), Query Frame = 1

Query: 1   MEST---GTTAAVILPTMLDNKKNLPREFVWPRGELAAESAGEREELKEPLIDLGGFRRG 60
           MEST   G  A +ILPTM+D KK LPREFVWPRGELA E   ER ELKEPLIDLGGFRRG
Sbjct: 1   MESTTTVGRVAGIILPTMVDKKKKLPREFVWPRGELAGE---ERGELKEPLIDLGGFRRG 60

Query: 61  EAEATAEAAAMVRVACRKHGLFQVTNHGVTAELIKAAYEEGEAIFKMPLVKKISVGKKPG 120
           E EATAEAAAMVR+AC KHG+FQVTNHGV  ELIKAAYEEGE IFKMPLVKKISVGKKPG
Sbjct: 61  EEEATAEAAAMVRMACMKHGVFQVTNHGVEEELIKAAYEEGEGIFKMPLVKKISVGKKPG 120

Query: 121 RVSGYSGAHADRFSSKLPWKETFSFEYSNDDSQRLHVLHYFKSLFGHGFENTGWVYQRYC 180
           RVSGYSGAHADRFSSKLPWKETFSFEYSNDDSQ LHVLH+FKSLFG  FENTGWVYQRYC
Sbjct: 121 RVSGYSGAHADRFSSKLPWKETFSFEYSNDDSQPLHVLHHFKSLFGCDFENTGWVYQRYC 180

Query: 181 EEMTRTALMIMELLAISLGVERFHYRKFFEDGRSIMRCNYYPPCENAGLTLGTGPHCDPT 240
           EEMTRTALMIMELLAISLGVER+HYRKFFEDG+SIMRCNYYPPCENA LTLGTGPHCDPT
Sbjct: 181 EEMTRTALMIMELLAISLGVERYHYRKFFEDGKSIMRCNYYPPCENASLTLGTGPHCDPT 240

Query: 241 SLTILHQDQVGGLEVFANNRWLSVKPRPDALVINIGDTFMALSNGAYKSCLHRAVVNRKR 300
           SLTILHQDQVGGLEVFANN WLSVKPRPDALVINIGDTFMALSNGAYKSCLHRAVVNRKR
Sbjct: 241 SLTILHQDQVGGLEVFANNAWLSVKPRPDALVINIGDTFMALSNGAYKSCLHRAVVNRKR 300

Query: 301 ERRSLVFFVCPKDDKVVRPPQDLVGRERPRQYPDFTWSDLLEFTQKHYRADVATLQSFVH 360
           ERRSLVFFVCPKDDKVVRPPQDLVGRE PRQYPDFTWS+LLEFTQKHYRADVATLQSFVH
Sbjct: 301 ERRSLVFFVCPKDDKVVRPPQDLVGREGPRQYPDFTWSELLEFTQKHYRADVATLQSFVH 360

Query: 361 WLQAKPHPPPIPF 371
           WLQAKPHPP IPF
Sbjct: 361 WLQAKPHPPKIPF 370

BLAST of Cla008413 vs. TrEMBL
Match: B9H4B0_POPTR (Gibberellin 20-oxidase family protein OS=Populus trichocarpa GN=POPTR_0005s06660g PE=3 SV=1)

HSP 1 Score: 524.6 bits (1350), Expect = 9.6e-146
Identity = 252/359 (70.19%), Postives = 290/359 (80.78%), Query Frame = 1

Query: 6   TTAAVILPTMLDNKKNLPREFVWPRGELAAESAGEREELKEPLIDLGGFRRGEAEATAEA 65
           T A V+  + L  + N+P EF+WP G+L       ++ELKEPLIDL GF +G+  ATA+A
Sbjct: 25  TDAPVLYSSFLQKQANMPTEFIWPIGDLVH----NQDELKEPLIDLDGFLKGDERATADA 84

Query: 66  AAMVRVACRKHGLFQVTNHGVTAELIKAAYEEGEAIFKMPLVKKISVGKKPGRVSGYSGA 125
           A +VR AC  HG FQV NHGV   LI AA+EE + IFK+PL KK+S  +KPG VSGYSGA
Sbjct: 85  AELVRTACLNHGFFQVINHGVDIGLIHAAHEEIDKIFKLPLDKKLSTRRKPGDVSGYSGA 144

Query: 126 HADRFSSKLPWKETFSFEYSNDDSQRLHVLHYFKSLFGHGFENTGWVYQRYCEEMTRTAL 185
           HA R+SSKLPWKETFSF Y  DD     V+ YFKS+ G  FE+TGWVYQRYCE M + +L
Sbjct: 145 HAHRYSSKLPWKETFSFGYHGDDDSEPLVVDYFKSVLGENFEHTGWVYQRYCEAMKKVSL 204

Query: 186 MIMELLAISLGVERFHYRKFFEDGRSIMRCNYYPPCENAGLTLGTGPHCDPTSLTILHQD 245
           +I ELL ISLGV+R HYRKFFEDG SIMRCN YPPC N+ LTLGTGPHCDPTSLTILHQD
Sbjct: 205 VIFELLGISLGVDRLHYRKFFEDGSSIMRCNNYPPCNNSSLTLGTGPHCDPTSLTILHQD 264

Query: 246 QVGGLEVFANNRWLSVKPRPDALVINIGDTFMALSNGAYKSCLHRAVVNRKRERRSLVFF 305
           QVGGLEVFANN+W +++PRPDALV+NIGDTF ALSNG Y+SCLHRAVVNR+RER+SLVFF
Sbjct: 265 QVGGLEVFANNKWQAIRPRPDALVVNIGDTFTALSNGRYQSCLHRAVVNRERERKSLVFF 324

Query: 306 VCPKDDKVVRPPQDLVGRERPRQYPDFTWSDLLEFTQKHYRADVATLQSFVHW-LQAKP 364
           V PK++KVVRPPQDLV RE PR+YPDFTWSDLLEFTQKHYRADVATLQSF+ W L AKP
Sbjct: 325 VSPKEEKVVRPPQDLVCREGPRKYPDFTWSDLLEFTQKHYRADVATLQSFIQWLLSAKP 379

BLAST of Cla008413 vs. TrEMBL
Match: B9HEX6_POPTR (Gibberellin 20-oxidase family protein OS=Populus trichocarpa GN=POPTR_0007s04360g PE=3 SV=1)

HSP 1 Score: 518.5 bits (1334), Expect = 6.9e-144
Identity = 250/356 (70.22%), Postives = 286/356 (80.34%), Query Frame = 1

Query: 6   TTAAVILPTMLDNKKNLPREFVWPRGELAAESAGEREELKEPLIDLGGFRRGEAEATAEA 65
           T A V   + L  + +LP EF+WP G+L        +ELKEP+IDL GF +G+  ATA+A
Sbjct: 21  TGALVFDSSFLQKQASLPTEFMWPHGDLVHNE----DELKEPMIDLEGFLKGDEVATAQA 80

Query: 66  AAMVRVACRKHGLFQVTNHGVTAELIKAAYEEGEAIFKMPLVKKISVGKKPGRVSGYSGA 125
           A +VR AC  HG FQVTNHGV   LI +A+EE   IFK+PL KK+SV +KPG VSGYSGA
Sbjct: 81  AELVRTACLNHGFFQVTNHGVDISLIHSAHEEIGKIFKLPLDKKLSVRRKPGDVSGYSGA 140

Query: 126 HADRFSSKLPWKETFSFEYSNDDSQRLHVLHYFKSLFGHGFENTGWVYQRYCEEMTRTAL 185
           HA R+SSKLPWKETFSF Y  DD     V+ YFKS+ G  FE+TGWVYQ YCE M + +L
Sbjct: 141 HAHRYSSKLPWKETFSFGYHGDDDSVPLVVDYFKSVLGKDFEHTGWVYQSYCEAMKKVSL 200

Query: 186 MIMELLAISLGVERFHYRKFFEDGRSIMRCNYYPPCENAGLTLGTGPHCDPTSLTILHQD 245
           +I ELLAISLGV+R HYRKFFEDG SIMRCNYYPPC N+ LTLGTGPHCDPTSLTILHQD
Sbjct: 201 VIFELLAISLGVDRLHYRKFFEDGSSIMRCNYYPPCNNSTLTLGTGPHCDPTSLTILHQD 260

Query: 246 QVGGLEVFANNRWLSVKPRPDALVINIGDTFMALSNGAYKSCLHRAVVNRKRERRSLVFF 305
           QVGGL+VF+NN+WL+++PRPDALV+NIGDTFMALSNG YKSCLHRAVVNR  ERRSLVFF
Sbjct: 261 QVGGLQVFSNNKWLAIRPRPDALVVNIGDTFMALSNGRYKSCLHRAVVNRDSERRSLVFF 320

Query: 306 VCPKDDKVVRPPQDLVGRERPRQYPDFTWSDLLEFTQKHYRADVATLQSFVHWLQA 362
           V PK++KVVRPPQDLV RE  R YPDFTWSDLLEFTQKHYRADVATLQSF+ WL +
Sbjct: 321 VSPKEEKVVRPPQDLVSREGQRIYPDFTWSDLLEFTQKHYRADVATLQSFIQWLSS 372

BLAST of Cla008413 vs. TrEMBL
Match: A0A061DRN7_THECC (Gibberellin 20-oxidase, putative OS=Theobroma cacao GN=TCM_001532 PE=3 SV=1)

HSP 1 Score: 511.1 bits (1315), Expect = 1.1e-141
Identity = 246/347 (70.89%), Postives = 281/347 (80.98%), Query Frame = 1

Query: 13  PTMLDNKKNLPREFVWPRGELAAESAGEREELKEPLIDLGGFRRGEAEATAEAAAMVRVA 72
           P+ L  + N+P EFVWP  +L       +EEL EPLIDL GF +G+ EAT+ A  +VR A
Sbjct: 68  PSKLQKQANIPAEFVWPHVDLVHT----QEELNEPLIDLEGFIKGDDEATSHAVDLVRSA 127

Query: 73  CRKHGLFQVTNHGVTAELIKAAYEEGEAIFKMPLVKKISVGKKPGRVSGYSGAHADRFSS 132
           C  HG FQVTNHGV A LI+AAYEE + +FKMPL KK+S  +KPG  SG+S AHADRFSS
Sbjct: 128 CLNHGFFQVTNHGVDATLIQAAYEEIDPVFKMPLNKKLSFQRKPGDFSGFSAAHADRFSS 187

Query: 133 KLPWKETFSFEYSNDDSQRLHVLHYFKSLFGHGFENTGWVYQRYCEEMTRTALMIMELLA 192
           KLPWKETFSF Y  ++S  + V+ YF+S  G  FE+TGW+YQ YCE+M   +L+I ELLA
Sbjct: 188 KLPWKETFSFGYHGNNSDPV-VVDYFRSALGEDFEHTGWIYQNYCEKMRELSLVIFELLA 247

Query: 193 ISLGVERFHYRKFFEDGRSIMRCNYYPPCENAGLTLGTGPHCDPTSLTILHQDQVGGLEV 252
           +SLGV+R HY KFFEDG SIMRCNYYPPC N+GLTLGTGPHCDPTSLTILHQDQVGGLEV
Sbjct: 248 VSLGVDRMHYHKFFEDGNSIMRCNYYPPCNNSGLTLGTGPHCDPTSLTILHQDQVGGLEV 307

Query: 253 FANNRWLSVKPRPDALVINIGDTFMALSNGAYKSCLHRAVVNRKRERRSLVFFVCPKDDK 312
           FANN+W +V+PRPDALVINIGDTFMAL NG YKSCLHRAVVN +R RRSLV+FVCPK+DK
Sbjct: 308 FANNKWQTVRPRPDALVINIGDTFMALCNGRYKSCLHRAVVNSERVRRSLVYFVCPKEDK 367

Query: 313 VVRPPQDLVGRERPRQYPDFTWSDLLEFTQKHYRADVATLQSFVHWL 360
           VVRPPQDLV R+ PR YPDFTWSDLLEFTQKHYRADV TLQSF  WL
Sbjct: 368 VVRPPQDLVCRDVPRLYPDFTWSDLLEFTQKHYRADVDTLQSFFPWL 409

BLAST of Cla008413 vs. TrEMBL
Match: A0A0B0MMS7_GOSAR (Uncharacterized protein OS=Gossypium arboreum GN=F383_17958 PE=3 SV=1)

HSP 1 Score: 511.1 bits (1315), Expect = 1.1e-141
Identity = 246/361 (68.14%), Postives = 288/361 (79.78%), Query Frame = 1

Query: 1   MESTGTTAAVIL-PTMLDNKKNLPREFVWPRGELAAESAGEREELKEPLIDLGGFRRGEA 60
           +E    T  V+  P+ L N+ +LP EF+WP G+L       +EEL EPLIDLGGF +G+ 
Sbjct: 20  IEPRDETGGVLFDPSKLQNQSSLPSEFIWPCGDLVHT----QEELNEPLIDLGGFIKGDE 79

Query: 61  EATAEAAAMVRVACRKHGLFQVTNHGVTAELIKAAYEEGEAIFKMPLVKKISVGKKPGRV 120
           EATA A  +V+ AC KHG FQVTNHGV + LI+AAY+E +A+FK+PL KK+S  +KPG  
Sbjct: 80  EATAHAVGLVKTACSKHGFFQVTNHGVDSNLIQAAYQEIDAVFKLPLNKKLSFQRKPGGF 139

Query: 121 SGYSGAHADRFSSKLPWKETFSFEYSNDDSQRLHVLHYFKSLFGHGFENTGWVYQRYCEE 180
           SGYS AHADRFS+KLPWKETFSF Y   +S    V+HYF S  G  FE TGW+YQ+YCE+
Sbjct: 140 SGYSAAHADRFSAKLPWKETFSFGYQGLNSDP-SVVHYFSSALGEDFEQTGWIYQKYCEK 199

Query: 181 MTRTALMIMELLAISLGVERFHYRKFFEDGRSIMRCNYYPPCENAGLTLGTGPHCDPTSL 240
           M+  +L+I ELLAISLG++R HYRKFFEDG SIMRCNYYPPC N+GLTLGTGPH DPTSL
Sbjct: 200 MSELSLVIFELLAISLGIDRLHYRKFFEDGNSIMRCNYYPPCNNSGLTLGTGPHSDPTSL 259

Query: 241 TILHQDQVGGLEVFANNRWLSVKPRPDALVINIGDTFMALSNGAYKSCLHRAVVNRKRER 300
           TILHQDQVGGLEVF NN+W +V+PR DA VINIGDTFMAL NG YKSCLHRAVVN++RER
Sbjct: 260 TILHQDQVGGLEVFNNNKWYAVRPRQDAFVINIGDTFMALCNGRYKSCLHRAVVNKERER 319

Query: 301 RSLVFFVCPKDDKVVRPPQDLVGRE-RPRQYPDFTWSDLLEFTQKHYRADVATLQSFVHW 360
           RSLV+FVCPK+DK+VRPPQDL+ R   PR YPDFTWSDLL+FTQ HYRADVATLQSF  W
Sbjct: 320 RSLVYFVCPKEDKIVRPPQDLMCRSGGPRVYPDFTWSDLLDFTQNHYRADVATLQSFFPW 375

BLAST of Cla008413 vs. NCBI nr
Match: gi|778717666|ref|XP_004143402.2| (PREDICTED: gibberellin 20 oxidase 2-like [Cucumis sativus])

HSP 1 Score: 696.4 bits (1796), Expect = 2.6e-197
Identity = 340/373 (91.15%), Postives = 348/373 (93.30%), Query Frame = 1

Query: 1   MEST---GTTAAVILPTMLDNKKNLPREFVWPRGELAAESAGEREELKEPLIDLGGFRRG 60
           MEST   G  A +ILPTM+D KK LPREFVWPRGELA E   ER ELKEPLIDLGGFRRG
Sbjct: 1   MESTTTVGRVAGIILPTMVDKKKKLPREFVWPRGELAGE---ERGELKEPLIDLGGFRRG 60

Query: 61  EAEATAEAAAMVRVACRKHGLFQVTNHGVTAELIKAAYEEGEAIFKMPLVKKISVGKKPG 120
           E EATAEAAAMVR+AC KHG+FQVTNHGV  ELIKAAYEEGE IFKMPLVKKISVGKKPG
Sbjct: 61  EEEATAEAAAMVRMACMKHGVFQVTNHGVEEELIKAAYEEGEGIFKMPLVKKISVGKKPG 120

Query: 121 RVSGYSGAHADRFSSKLPWKETFSFEYSNDDSQRLHVLHYFKSLFGHGFENTGWVYQRYC 180
           RVSGYSGAHADRFSSKLPWKETFSFEYSNDDSQ LHVLH+FKSLFG  FENTGWVYQRYC
Sbjct: 121 RVSGYSGAHADRFSSKLPWKETFSFEYSNDDSQPLHVLHHFKSLFGCDFENTGWVYQRYC 180

Query: 181 EEMTRTALMIMELLAISLGVERFHYRKFFEDGRSIMRCNYYPPCENAGLTLGTGPHCDPT 240
           EEMTRTALMIMELLAISLGVER+HYRKFFEDG+SIMRCNYYPPCENA LTLGTGPHCDPT
Sbjct: 181 EEMTRTALMIMELLAISLGVERYHYRKFFEDGKSIMRCNYYPPCENASLTLGTGPHCDPT 240

Query: 241 SLTILHQDQVGGLEVFANNRWLSVKPRPDALVINIGDTFMALSNGAYKSCLHRAVVNRKR 300
           SLTILHQDQVGGLEVFANN WLSVKPRPDALVINIGDTFMALSNGAYKSCLHRAVVNRKR
Sbjct: 241 SLTILHQDQVGGLEVFANNAWLSVKPRPDALVINIGDTFMALSNGAYKSCLHRAVVNRKR 300

Query: 301 ERRSLVFFVCPKDDKVVRPPQDLVGRERPRQYPDFTWSDLLEFTQKHYRADVATLQSFVH 360
           ERRSLVFFVCPKDDKVVRPPQDLVGRE PRQYPDFTWS+LLEFTQKHYRADVATLQSFVH
Sbjct: 301 ERRSLVFFVCPKDDKVVRPPQDLVGREGPRQYPDFTWSELLEFTQKHYRADVATLQSFVH 360

Query: 361 WLQAKPHPPPIPF 371
           WLQAKPHPP IPF
Sbjct: 361 WLQAKPHPPKIPF 370

BLAST of Cla008413 vs. NCBI nr
Match: gi|659125355|ref|XP_008462644.1| (PREDICTED: gibberellin 20 oxidase 2-like [Cucumis melo])

HSP 1 Score: 695.3 bits (1793), Expect = 5.9e-197
Identity = 342/374 (91.44%), Postives = 348/374 (93.05%), Query Frame = 1

Query: 1   MEST---GTTAAVILPTMLDNKKNLPREFVWPRGELAAESAGEREELKEPLIDLGGFRRG 60
           MEST   G  A +ILPTM+D KK LPREFVWPRGELA E   EREELKEPLIDLGGFRRG
Sbjct: 1   MESTTTVGRVAGIILPTMVDKKKKLPREFVWPRGELAGE---EREELKEPLIDLGGFRRG 60

Query: 61  EAEATAEAAAMVRVACRKHGLFQVTNHGVTAELIKAAYEEGEAIFKMPLVKKISVGKKPG 120
           E EATAEAAAMVRVAC KHG+FQVTNHGV  ELIKAAYEEGE IFKMPLVKKISVGKKPG
Sbjct: 61  EEEATAEAAAMVRVACMKHGVFQVTNHGVEEELIKAAYEEGEGIFKMPLVKKISVGKKPG 120

Query: 121 RVSGYSGAHADRFSSKLPWKETFSFEYSNDDS-QRLHVLHYFKSLFGHGFENTGWVYQRY 180
           RVSGYSGAHADRFSSKLPWKETFSFEYSNDDS Q LHVLHYFKSLFG  FENTGWVYQRY
Sbjct: 121 RVSGYSGAHADRFSSKLPWKETFSFEYSNDDSDQPLHVLHYFKSLFGCDFENTGWVYQRY 180

Query: 181 CEEMTRTALMIMELLAISLGVERFHYRKFFEDGRSIMRCNYYPPCENAGLTLGTGPHCDP 240
           CEEMTR ALMIMELLAISLGVER+HYRKFFEDG+SIMRCNYYPPCENA LTLGTGPHCDP
Sbjct: 181 CEEMTRIALMIMELLAISLGVERYHYRKFFEDGKSIMRCNYYPPCENASLTLGTGPHCDP 240

Query: 241 TSLTILHQDQVGGLEVFANNRWLSVKPRPDALVINIGDTFMALSNGAYKSCLHRAVVNRK 300
           TSLTILHQDQVGGLEVFANN WLSVKPRPDALVINIGDTFMALSNGAYKSCLHRAVVNRK
Sbjct: 241 TSLTILHQDQVGGLEVFANNTWLSVKPRPDALVINIGDTFMALSNGAYKSCLHRAVVNRK 300

Query: 301 RERRSLVFFVCPKDDKVVRPPQDLVGRERPRQYPDFTWSDLLEFTQKHYRADVATLQSFV 360
           RERRSLVFFVCPKDDKVVRPPQDLVGRE PRQYPDFTWS+LLEFTQKHYRADVATLQSFV
Sbjct: 301 RERRSLVFFVCPKDDKVVRPPQDLVGREGPRQYPDFTWSELLEFTQKHYRADVATLQSFV 360

Query: 361 HWLQAKPHPPPIPF 371
           HWLQAKPHPP IPF
Sbjct: 361 HWLQAKPHPPKIPF 371

BLAST of Cla008413 vs. NCBI nr
Match: gi|224083474|ref|XP_002307041.1| (gibberellin 20-oxidase family protein [Populus trichocarpa])

HSP 1 Score: 524.6 bits (1350), Expect = 1.4e-145
Identity = 252/359 (70.19%), Postives = 290/359 (80.78%), Query Frame = 1

Query: 6   TTAAVILPTMLDNKKNLPREFVWPRGELAAESAGEREELKEPLIDLGGFRRGEAEATAEA 65
           T A V+  + L  + N+P EF+WP G+L       ++ELKEPLIDL GF +G+  ATA+A
Sbjct: 25  TDAPVLYSSFLQKQANMPTEFIWPIGDLVH----NQDELKEPLIDLDGFLKGDERATADA 84

Query: 66  AAMVRVACRKHGLFQVTNHGVTAELIKAAYEEGEAIFKMPLVKKISVGKKPGRVSGYSGA 125
           A +VR AC  HG FQV NHGV   LI AA+EE + IFK+PL KK+S  +KPG VSGYSGA
Sbjct: 85  AELVRTACLNHGFFQVINHGVDIGLIHAAHEEIDKIFKLPLDKKLSTRRKPGDVSGYSGA 144

Query: 126 HADRFSSKLPWKETFSFEYSNDDSQRLHVLHYFKSLFGHGFENTGWVYQRYCEEMTRTAL 185
           HA R+SSKLPWKETFSF Y  DD     V+ YFKS+ G  FE+TGWVYQRYCE M + +L
Sbjct: 145 HAHRYSSKLPWKETFSFGYHGDDDSEPLVVDYFKSVLGENFEHTGWVYQRYCEAMKKVSL 204

Query: 186 MIMELLAISLGVERFHYRKFFEDGRSIMRCNYYPPCENAGLTLGTGPHCDPTSLTILHQD 245
           +I ELL ISLGV+R HYRKFFEDG SIMRCN YPPC N+ LTLGTGPHCDPTSLTILHQD
Sbjct: 205 VIFELLGISLGVDRLHYRKFFEDGSSIMRCNNYPPCNNSSLTLGTGPHCDPTSLTILHQD 264

Query: 246 QVGGLEVFANNRWLSVKPRPDALVINIGDTFMALSNGAYKSCLHRAVVNRKRERRSLVFF 305
           QVGGLEVFANN+W +++PRPDALV+NIGDTF ALSNG Y+SCLHRAVVNR+RER+SLVFF
Sbjct: 265 QVGGLEVFANNKWQAIRPRPDALVVNIGDTFTALSNGRYQSCLHRAVVNRERERKSLVFF 324

Query: 306 VCPKDDKVVRPPQDLVGRERPRQYPDFTWSDLLEFTQKHYRADVATLQSFVHW-LQAKP 364
           V PK++KVVRPPQDLV RE PR+YPDFTWSDLLEFTQKHYRADVATLQSF+ W L AKP
Sbjct: 325 VSPKEEKVVRPPQDLVCREGPRKYPDFTWSDLLEFTQKHYRADVATLQSFIQWLLSAKP 379

BLAST of Cla008413 vs. NCBI nr
Match: gi|743861585|ref|XP_011031154.1| (PREDICTED: gibberellin 20 oxidase 1-B-like [Populus euphratica])

HSP 1 Score: 523.1 bits (1346), Expect = 4.0e-145
Identity = 251/359 (69.92%), Postives = 289/359 (80.50%), Query Frame = 1

Query: 6   TTAAVILPTMLDNKKNLPREFVWPRGELAAESAGEREELKEPLIDLGGFRRGEAEATAEA 65
           T A V+  + L  + N+P EF+WP G+L       ++ELKEPLIDL GF +G+  ATA+A
Sbjct: 25  TNAPVLYSSFLQKQANMPTEFIWPIGDLVH----NQDELKEPLIDLDGFLKGDERATADA 84

Query: 66  AAMVRVACRKHGLFQVTNHGVTAELIKAAYEEGEAIFKMPLVKKISVGKKPGRVSGYSGA 125
           A +VR AC  HG FQV NHGV   LI AA+EE + IFK+PL KK+S  +KPG VSGYSGA
Sbjct: 85  AELVRTACLNHGFFQVINHGVDIGLIHAAHEEIDKIFKLPLDKKLSTRRKPGDVSGYSGA 144

Query: 126 HADRFSSKLPWKETFSFEYSNDDSQRLHVLHYFKSLFGHGFENTGWVYQRYCEEMTRTAL 185
           HA R+SSKLPWKETFSF Y  DD     V+ YFKS+ G  FE+TGWVYQRYCE M + +L
Sbjct: 145 HAHRYSSKLPWKETFSFGYHGDDGSEPLVVDYFKSVLGENFEHTGWVYQRYCEAMKKVSL 204

Query: 186 MIMELLAISLGVERFHYRKFFEDGRSIMRCNYYPPCENAGLTLGTGPHCDPTSLTILHQD 245
           +I ELL ISLGV+R HYRKFFEDG SIMRCN YPPC N+ LTLGTGPHCDPTSLTILHQD
Sbjct: 205 VIFELLGISLGVDRLHYRKFFEDGSSIMRCNNYPPCNNSSLTLGTGPHCDPTSLTILHQD 264

Query: 246 QVGGLEVFANNRWLSVKPRPDALVINIGDTFMALSNGAYKSCLHRAVVNRKRERRSLVFF 305
           QVGGLEVFANN+W +++PRPDALV+NIGDTF ALSNG Y+SCLHRAVVNR+RER+SLVFF
Sbjct: 265 QVGGLEVFANNKWQAIRPRPDALVVNIGDTFTALSNGRYQSCLHRAVVNRERERKSLVFF 324

Query: 306 VCPKDDKVVRPPQDLVGRERPRQYPDFTWSDLLEFTQKHYRADVATLQSFVHW-LQAKP 364
           V PK++KVVRPP DLV RE PR+YPDFTWSDLLEFTQKHYRADVATLQSF+ W L AKP
Sbjct: 325 VSPKEEKVVRPPHDLVCREGPRKYPDFTWSDLLEFTQKHYRADVATLQSFIQWLLSAKP 379

BLAST of Cla008413 vs. NCBI nr
Match: gi|224096043|ref|XP_002310523.1| (gibberellin 20-oxidase family protein [Populus trichocarpa])

HSP 1 Score: 518.5 bits (1334), Expect = 9.8e-144
Identity = 250/356 (70.22%), Postives = 286/356 (80.34%), Query Frame = 1

Query: 6   TTAAVILPTMLDNKKNLPREFVWPRGELAAESAGEREELKEPLIDLGGFRRGEAEATAEA 65
           T A V   + L  + +LP EF+WP G+L        +ELKEP+IDL GF +G+  ATA+A
Sbjct: 21  TGALVFDSSFLQKQASLPTEFMWPHGDLVHNE----DELKEPMIDLEGFLKGDEVATAQA 80

Query: 66  AAMVRVACRKHGLFQVTNHGVTAELIKAAYEEGEAIFKMPLVKKISVGKKPGRVSGYSGA 125
           A +VR AC  HG FQVTNHGV   LI +A+EE   IFK+PL KK+SV +KPG VSGYSGA
Sbjct: 81  AELVRTACLNHGFFQVTNHGVDISLIHSAHEEIGKIFKLPLDKKLSVRRKPGDVSGYSGA 140

Query: 126 HADRFSSKLPWKETFSFEYSNDDSQRLHVLHYFKSLFGHGFENTGWVYQRYCEEMTRTAL 185
           HA R+SSKLPWKETFSF Y  DD     V+ YFKS+ G  FE+TGWVYQ YCE M + +L
Sbjct: 141 HAHRYSSKLPWKETFSFGYHGDDDSVPLVVDYFKSVLGKDFEHTGWVYQSYCEAMKKVSL 200

Query: 186 MIMELLAISLGVERFHYRKFFEDGRSIMRCNYYPPCENAGLTLGTGPHCDPTSLTILHQD 245
           +I ELLAISLGV+R HYRKFFEDG SIMRCNYYPPC N+ LTLGTGPHCDPTSLTILHQD
Sbjct: 201 VIFELLAISLGVDRLHYRKFFEDGSSIMRCNYYPPCNNSTLTLGTGPHCDPTSLTILHQD 260

Query: 246 QVGGLEVFANNRWLSVKPRPDALVINIGDTFMALSNGAYKSCLHRAVVNRKRERRSLVFF 305
           QVGGL+VF+NN+WL+++PRPDALV+NIGDTFMALSNG YKSCLHRAVVNR  ERRSLVFF
Sbjct: 261 QVGGLQVFSNNKWLAIRPRPDALVVNIGDTFMALSNGRYKSCLHRAVVNRDSERRSLVFF 320

Query: 306 VCPKDDKVVRPPQDLVGRERPRQYPDFTWSDLLEFTQKHYRADVATLQSFVHWLQA 362
           V PK++KVVRPPQDLV RE  R YPDFTWSDLLEFTQKHYRADVATLQSF+ WL +
Sbjct: 321 VSPKEEKVVRPPQDLVSREGQRIYPDFTWSDLLEFTQKHYRADVATLQSFIQWLSS 372

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
GAOX2_ORYSJ9.3e-11056.53Gibberellin 20 oxidase 2 OS=Oryza sativa subsp. japonica GN=20ox2 PE=1 SV=1[more]
GAOX2_ORYSI9.3e-11056.53Gibberellin 20 oxidase 2 OS=Oryza sativa subsp. indica GN=20ox2 PE=1 SV=1[more]
GAOX2_ARATH2.3e-10852.33Gibberellin 20 oxidase 2 OS=Arabidopsis thaliana GN=GA20OX2 PE=2 SV=1[more]
GAOX1_ARATH1.1e-10754.39Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana GN=GA20OX1 PE=2 SV=2[more]
GAO1B_WHEAT4.3e-10755.65Gibberellin 20 oxidase 1-B OS=Triticum aestivum GN=GA20ox1B PE=2 SV=1[more]
Match NameE-valueIdentityDescription
S0BDZ8_CUCSA1.8e-19791.15GA 20-oxidase OS=Cucumis sativus GN=GA20ox2 PE=2 SV=1[more]
B9H4B0_POPTR9.6e-14670.19Gibberellin 20-oxidase family protein OS=Populus trichocarpa GN=POPTR_0005s06660... [more]
B9HEX6_POPTR6.9e-14470.22Gibberellin 20-oxidase family protein OS=Populus trichocarpa GN=POPTR_0007s04360... [more]
A0A061DRN7_THECC1.1e-14170.89Gibberellin 20-oxidase, putative OS=Theobroma cacao GN=TCM_001532 PE=3 SV=1[more]
A0A0B0MMS7_GOSAR1.1e-14168.14Uncharacterized protein OS=Gossypium arboreum GN=F383_17958 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
gi|778717666|ref|XP_004143402.2|2.6e-19791.15PREDICTED: gibberellin 20 oxidase 2-like [Cucumis sativus][more]
gi|659125355|ref|XP_008462644.1|5.9e-19791.44PREDICTED: gibberellin 20 oxidase 2-like [Cucumis melo][more]
gi|224083474|ref|XP_002307041.1|1.4e-14570.19gibberellin 20-oxidase family protein [Populus trichocarpa][more]
gi|743861585|ref|XP_011031154.1|4.0e-14569.92PREDICTED: gibberellin 20 oxidase 1-B-like [Populus euphratica][more]
gi|224096043|ref|XP_002310523.1|9.8e-14470.22gibberellin 20-oxidase family protein [Populus trichocarpa][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR002283Isopenicillin N synthase
IPR005123Oxoglu/Fe-dep_dioxygenase
IPR026992DIOX_N
IPR027443IPNS-like
Vocabulary: Molecular Function
TermDefinition
GO:0005506iron ion binding
GO:0016491oxidoreductase activity
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016102 diterpenoid biosynthetic process
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0008150 biological_process
biological_process GO:0009908 flower development
biological_process GO:0009686 gibberellin biosynthetic process
biological_process GO:0009826 unidimensional cell growth
cellular_component GO:0005575 cellular_component
molecular_function GO:0016707 gibberellin 3-beta-dioxygenase activity
molecular_function GO:0005506 iron ion binding
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0047927 gibberellin-44 dioxygenase activity
molecular_function GO:0051213 dioxygenase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0003674 molecular_function
molecular_function GO:0045544 gibberellin 20-oxidase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cla008413Cla008413.1mRNA


Analysis Name: InterPro Annotations of watermelon (97103)
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002283Isopenicillin N synthasePRINTSPR00682IPNSYNTHASEcoord: 268..294
score: 2.3E-7coord: 233..254
score: 2.3E-7coord: 69..86
score: 2.
IPR005123Oxoglutarate/iron-dependent dioxygenasePFAMPF031712OG-FeII_Oxycoord: 211..308
score: 4.0
IPR005123Oxoglutarate/iron-dependent dioxygenasePROFILEPS51471FE2OG_OXYcoord: 209..308
score: 14
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 47..149
score: 3.6
IPR027443Isopenicillin N synthase-likeGENE3DG3DSA:2.60.120.330coord: 18..353
score: 9.7
NoneNo IPR availablePANTHERPTHR10209OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEINcoord: 1..351
score: 6.1E
NoneNo IPR availablePANTHERPTHR10209:SF129SUBFAMILY NOT NAMEDcoord: 1..351
score: 6.1E
NoneNo IPR availableunknownSSF51197Clavaminate synthase-likecoord: 15..347
score: 2.54