Cp4.1LG09g04300 (gene) Cucurbita pepo (Zucchini)

Overview
NameCp4.1LG09g04300
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
DescriptionSc-RNase
LocationCp4.1LG09 : 2784909 .. 2786946 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TATTGCGGGTCCACCGGGTCCACGGGTTATATTCTCTCAACCCGACACTACCACGCTAGAAACCCTAAAACCCTAAAGTGTTTCTTCTTTTTCTTTAGCCGTCGCCTCCTCCGCCTCATTTTCCCCATTCCCTTCCATTTCCCTCTGTACGTACCACTTCATTCTCTTCGCCGGAAAAGCTATGGCGCCGTCTGGTAATAATCACTCCTGTGCATCAAAGTGTTCCTTGGCTTGTAAGTCCTCTCTTCGCAGAATAATGGTCGTGTTAATCCTCTGCGTGTTTGCTCGTTTTTCATCGTTTGCTAGATCGAAGGTTACTGTCTTCCATCCTTTTCTTTCTTCATTTCGCGGTTCTGGTCCTTGGTGAGGTCTGAGGGTTTGCTGAAGCGTAGAACTTCAGGTATGGTCGTGGATCCGTTGGAGGTGCGGGTTCTCTCTCTCTCGATTTTTCTGCTCTTTTCTTCCTCTGTGCAGGTGTTCGAGACATTTGCTGAAGGCCGGAGGATGGAGGGAACGTTTGGGGACGATCCGCCGAGGTTGTGGACGTGAGATCCCTCGCCGGCTACATCGGTAATTTTTTATTTTTATTTTTTAATAATTCTGTTTCCTTACCAAAAATAAAAAAATAAAAAAGGTTAAAATTAGGAGATTCTCACTTTTTTTTTTTTTTTTTTAAATTAAAAAATCTGATTCTGTTTAGTTTTATTTATTTTATTCATTTATTCTCACTGGAAATGGAAATTTGGGTGCTCAGTAGATGTTTACTCTTTAGACATTTCCACACTCACCGAGTAATGGAAATTGGGCGAGTAGGGTTTGTTTGCTTCAGACATTTCCACATTCTACAAGTATGAGCTTCTACATTTAGCCAAGGAAGAGCTTCCATATTTGTTGTTTACTGAGTTTACTGAGTGAGGTGCATATCCATCCACACTATAAGTATGGATGGGTTGCTGCGATGTTCATCACCACATCTCCCTCTCTTCCTTTCTCTACTTCTTCTGCTATGGCCTTTATTCCCTCCAATCTTCTCCCCATTTGCATCTCACTCCTTTTGTTTTTCAATTCTGTGTATTCCTTCGACTACTTTCAAATGGTTTTACAATGGCCGCCCGCTACTTGTAAGACTAAAGATGTAGTCTGTTATACTGCACCTCCTAAGATGTTCACCATACATGGTCTCTGGCCTAGTTATTTCTCGGGGGATATGGTTGTTTGTACTTCTAAGGCAAAATTGGACCCAACAAAACAGGTATCTTCTGTGTCATTACAACTCATTAGTGTTTATGATTTTATTTAATTTAATTAGTGCATCCTAAGTGGATTTTTTATTTTTCGTTTACAGCTTGCTCCACTCAAAACACAGCTAGAAATGTATTGGCCAGACGTGATAAGCGGCGACCATGAAACATTTTGGAAACACGAATGGGATAAGCACGGTAAATGTTCATTATTTACCCAATATCAATATTTTGAAGTATCTCTCCGTATTCGATTGAACTCTACCTTCAATATATTAGCCATCTTGAATGCCGCCCATCTTAACCCGGATGTCAACATATCTCTGAATTATCTTGTTGTTGAAAATGCTATTAAGATTGCAATAGGAAAAAGGCCTGCCCTCCACTGCAACAAGGATACATCTAAACAGAAACAATTATTCGAAATTATTTTATGTTTTGATACGGATGCAGTGACTTTGATCGATTGTAGCTCAGAACTTAGAACAAGTTGCATAACTCTTTCCACGATTCACGATGAATTGTAGTTTTTTTTTTTTTTTCCTATCTGAAAAAAATACTGGAAAGTTATGTTTGTTTAAATATATGTTATGTTTATCTTCGTAGAAAATATGTTATGTTATGTTTAAATATATATGTTAGTCTCTTATTTTATTTTAAATATTATCCATATAAATGCATAATAAAATTTGGAATAAATTGATGTTTGGTTTTTTTGAATTAATCGATGAGAATGAATGTTTTTAACTTTCTTAATAAAAAATATATTTAATTTAAATTATTAGATGGGGTTTTAT

mRNA sequence

TATTGCGGGTCCACCGGGTCCACGGGTTATATTCTCTCAACCCGACACTACCACGCTAGAAACCCTAAAACCCTAAAGTGTTTCTTCTTTTTCTTTAGCCGTCGCCTCCTCCGCCTCATTTTCCCCATTCCCTTCCATTTCCCTCTGTACGTACCACTTCATTCTCTTCGCCGGAAAAGCTATGGCGCCGTCTGATCGAAGGTTACTGTCTTCCATCCTTTTCTTTCTTCATTTCGCGGTTCTGGTCCTTGGTGAGGTCTGAGGGTTTGCTGAAGCGTAGAACTTCAGGTGTTCGAGACATTTGCTGAAGGCCGGAGGATGGAGGGAACGTTTGGGGACGATCCGCCGAGGTTGTGGACGTGAGATCCCTCGCCGGCTACATCGGTAATTTTTTATTTTTATTTTTTAATAATTCTGTTTCCTTACCAAAAATAAAAAAATAAAAAAGGTTAAAATTAGGAGATTCTCACTTTTTTTTTTTTTTTTTTAAATTAAAAAATCTGATTCTGTTTAGTTTTATTTATTTTATTCATTTATTCTCACTGGAAATGGAAATTTGGGTGCTCAGTAGATGTTTACTCTTTAGACATTTCCACACTCACCGAGTAATGGAAATTGGGCGAGTAGGGTTTGTTTGCTTCAGACATTTCCACATTCTACAAGTATGAGCTTCTACATTTAGCCAAGGAAGAGCTTCCATATTTGTTGTTTACTGAGTTTACTGAGTGAGGTGCATATCCATCCACACTATAAGTATGGATGGGTTGCTGCGATGTTCATCACCACATCTCCCTCTCTTCCTTTCTCTACTTCTTCTGCTATGGCCTTTATTCCCTCCAATCTTCTCCCCATTTGCATCTCACTCCTTTTGTTTTTCAATTCTGTGTATTCCTTCGACTACTTTCAAATGGTTTTACAATGGCCGCCCGCTACTTGTAAGACTAAAGATGTAGTCTGTTATACTGCACCTCCTAAGATGTTCACCATACATGGTCTCTGGCCTAGTTATTTCTCGGGGGATATGGTTGTTTGTACTTCTAAGGCAAAATTGGACCCAACAAAACAGCTTGCTCCACTCAAAACACAGCTAGAAATGTATTGGCCAGACGTGATAAGCGGCGACCATGAAACATTTTGGAAACACGAATGGGATAAGCACGCCATCTTGAATGCCGCCCATCTTAACCCGGATGTCAACATATCTCTGAATTATCTTGTTGTTGAAAATGCTATTAAGATTGCAATAGGAAAAAGGCCTGCCCTCCACTGCAACAAGGATACATCTAAACAGAAACAATTATTCGAAATTATTTTATGTTTTGATACGGATGCAGTGACTTTGATCGATTGTAGCTCAGAACTTAGAACAAGTTGCATAACTCTTTCCACGATTCACGATGAATTGTAGTTTTTTTTTTTTTTTCCTATCTGAAAAAAATACTGGAAAGTTATGTTTGTTTAAATATATGTTATGTTTATCTTCGTAGAAAATATGTTATGTTATGTTTAAATATATATGTTAGTCTCTTATTTTATTTTAAATATTATCCATATAAATGCATAATAAAATTTGGAATAAATTGATGTTTGGTTTTTTTGAATTAATCGATGAGAATGAATGTTTTTAACTTTCTTAATAAAAAATATATTTAATTTAAATTATTAGATGGGGTTTTAT

Coding sequence (CDS)

ATGTTCATCACCACATCTCCCTCTCTTCCTTTCTCTACTTCTTCTGCTATGGCCTTTATTCCCTCCAATCTTCTCCCCATTTGCATCTCACTCCTTTTGTTTTTCAATTCTGTGTATTCCTTCGACTACTTTCAAATGGTTTTACAATGGCCGCCCGCTACTTGTAAGACTAAAGATGTAGTCTGTTATACTGCACCTCCTAAGATGTTCACCATACATGGTCTCTGGCCTAGTTATTTCTCGGGGGATATGGTTGTTTGTACTTCTAAGGCAAAATTGGACCCAACAAAACAGCTTGCTCCACTCAAAACACAGCTAGAAATGTATTGGCCAGACGTGATAAGCGGCGACCATGAAACATTTTGGAAACACGAATGGGATAAGCACGCCATCTTGAATGCCGCCCATCTTAACCCGGATGTCAACATATCTCTGAATTATCTTGTTGTTGAAAATGCTATTAAGATTGCAATAGGAAAAAGGCCTGCCCTCCACTGCAACAAGGATACATCTAAACAGAAACAATTATTCGAAATTATTTTATGTTTTGATACGGATGCAGTGACTTTGATCGATTGTAGCTCAGAACTTAGAACAAGTTGCATAACTCTTTCCACGATTCACGATGAATTGTAG

Protein sequence

MFITTSPSLPFSTSSAMAFIPSNLLPICISLLLFFNSVYSFDYFQMVLQWPPATCKTKDVVCYTAPPKMFTIHGLWPSYFSGDMVVCTSKAKLDPTKQLAPLKTQLEMYWPDVISGDHETFWKHEWDKHAILNAAHLNPDVNISLNYLVVENAIKIAIGKRPALHCNKDTSKQKQLFEIILCFDTDAVTLIDCSSELRTSCITLSTIHDEL
Homology
[Close]
BLAST of Cp4.1LG09g04300 vs. Swiss-Prot
Match: RNLE_SOLLC (Extracellular ribonuclease LE OS=Solanum lycopersicum PE=1 SV=2)

HSP 1 Score: 108.6 bits (270), Expect = 8.4e-23
Identity = 70/220 (31.82%), Postives = 101/220 (45.91%), Query Frame = 1 

Query: 12  STSSAMAFIPSNLLPICISLLLFFNSVYSFDYFQMVLQWPPATCKTKDVVCYTA---PPK 71
           S S+   F+   ++  C+S+L   N+   FD+F  V QWP + C TK   CY     P  
Sbjct: 3   SNSAFSLFLILLIITQCLSVL---NAAKDFDFFYFVQQWPGSYCDTKQSCCYPTTGKPAA 62

Query: 72  MFTIHGLWPSYFSGDMVVCTSKAKLDPTK-----QLAPLKTQLEMYWPDVI--SGDHETF 131
            F IHGLWP+   G     T  +  DP       Q++ L + ++  WP +   SG   TF
Sbjct: 63  DFGIHGLWPNNNDG-----TYPSNCDPNSPYDQSQISDLISSMQQNWPTLACPSGSGSTF 122

Query: 132 WKHEWDKH----------------------------AILNAAHLNPDVNISLNYLVVENA 191
           W HEW+KH                            +IL  A ++PD   S + + + NA
Sbjct: 123 WSHEWEKHGTCAESVLTNQHAYFKKALDLKNQIDLLSILQGADIHPDGE-SYDLVNIRNA 182

Query: 192 IKIAIGKRPALHCNKDTSKQKQLFEIILCFDTDAVTLIDC 194
           IK AIG  P + CN D S   QL+++ +C D    +LI+C
Sbjct: 183 IKSAIGYTPWIQCNVDQSGNSQLYQVYICVDGSGSSLIEC 213

[Close]
BLAST of Cp4.1LG09g04300 vs. Swiss-Prot
Match: RNS1_ARATH (Ribonuclease 1 OS=Arabidopsis thaliana GN=RNS1 PE=1 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 1.6e-21
Identity = 68/210 (32.38%), Postives = 96/210 (45.71%), Query Frame = 1 

Query: 24  LLPIC-ISLLLFFNSVYS-------FDYFQMVLQWPPATCKTKDVVCYT---APPKMFTI 83
           L  +C ISLL+   SV+S       FD+F  V QWP + C T+   CY     P   F I
Sbjct: 5   LASLCLISLLVILPSVFSASSSSEDFDFFYFVQQWPGSYCDTQKKCCYPNSGKPAADFGI 64

Query: 84  HGLWPSYFSGDMVVCTSKAKLDPTKQLAPLKTQLEMYWPDVI--SGDHETFWKHEWDKHA 143
           HGLWP+Y  G        +K   +  ++ L T ++  WP +   SG  E FW+HEW+KH 
Sbjct: 65  HGLWPNYKDGTYPSNCDASKPFDSSTISDLLTSMKKSWPTLACPSGSGEAFWEHEWEKHG 124

Query: 144 I---------------------------LNAAHLNPDVNISLNYLVVENAIKIAIGKRPA 194
                                       L  A +NPD   S +   + ++IK +IG  P 
Sbjct: 125 TCSESVIDQHEYFQTALNLKQKTNLLGALTKAGINPDGK-SYSLESIRDSIKESIGFTPW 184

[Close]
BLAST of Cp4.1LG09g04300 vs. Swiss-Prot
Match: RNS3_ARATH (Ribonuclease 3 OS=Arabidopsis thaliana GN=RNS3 PE=2 SV=1)

HSP 1 Score: 103.6 bits (257), Expect = 2.7e-21
Identity = 62/186 (33.33%), Postives = 84/186 (45.16%), Query Frame = 1 

Query: 41  FDYFQMVLQWPPATCKTKDVVCYTA---PPKMFTIHGLWPSYFSGDMVV-CTSKAKLDPT 100
           FD+F  VLQWP A C ++   CY     P   F IHGLWP+Y +G     C   ++ D  
Sbjct: 22  FDFFYFVLQWPGAYCDSRHSCCYPQTGKPAADFGIHGLWPNYKTGGWPQNCNPDSRFDDL 81

Query: 101 KQLAPLKTQLEMYWPDVI--SGDHETFWKHEWDKHAI----------------------- 160
           + ++ L + L+  WP +   S D   FW HEW+KH                         
Sbjct: 82  R-VSDLMSDLQREWPTLSCPSNDGMKFWTHEWEKHGTCAESELDQHDYFEAGLKLKQKAN 141

Query: 161 ----LNAAHLNPDVNISLNYLVVENAIKIAIGKRPALHCNKDTSKQKQLFEIILCFDTDA 194
               L  A + PD         +EN IK  +G  P + CNKD+S   QL++I LC DT A
Sbjct: 142 LLHALTNAGIKPDDKF-YEMKDIENTIKQVVGFAPGIECNKDSSHNSQLYQIYLCVDTSA 201

[Close]
BLAST of Cp4.1LG09g04300 vs. Swiss-Prot
Match: RNS2_ANTHI (Ribonuclease S-2 OS=Antirrhinum hispanicum GN=S2 PE=2 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 7.4e-11
Identity = 63/205 (30.73%), Postives = 82/205 (40.00%), Query Frame = 1 

Query: 24  LLPICISLLLFFNSVYS--FDYFQMVLQWPPATCKTKDVVC-YTAPPKMFTIHGLWPSYF 83
           LL  CI  L  F S  +  FDYF++VLQWP + C  K   C  T  P  FTIHGLWP   
Sbjct: 14  LLVGCIVHLSNFCSTTTAQFDYFKLVLQWPNSYCSLKTTHCPRTRLPSQFTIHGLWPDNK 73

Query: 84  SGDMVVC--TSKAKLDPTKQLAPLKTQLEMYWPDVISGDH----ETFWKHEWDKHA---- 143
           S  +  C  TS   L  T +   L   L ++WPD+         + FW  +W KH     
Sbjct: 74  SWPLSNCRDTSADVLKITDK--GLIQDLAVHWPDLTRRQRKVPGQKFWVTQWKKHGACAL 133

Query: 144 --------ILNAAHLNPDVNI--------------SLNYLVVENAIKIAIGKRPALHCNK 194
                    + A  L    N+               ++   V  AI    G    L C  
Sbjct: 134 PMYSFNDYFVKALELKKRNNVLDMLSRKSLTPGDQRVDVSDVNGAITKVTGGIAILKC-- 193

[Close]
BLAST of Cp4.1LG09g04300 vs. Swiss-Prot
Match: RNT2_HUMAN (Ribonuclease T2 OS=Homo sapiens GN=RNASET2 PE=1 SV=2)

HSP 1 Score: 64.3 bits (155), Expect = 1.8e-09
Identity = 35/88 (39.77%), Postives = 42/88 (47.73%), Query Frame = 1 

Query: 46  MVLQWPPATCKTKDVVCYTAPPKMFTIHGLWPSYFSGDMVVCTSK--AKLDPTKQLAPLK 105
           MV  WP   C+     C   PP  +TIHGLWP    G    C       L+  K L P  
Sbjct: 39  MVQHWPETVCEKIQNDCRD-PPDYWTIHGLWPDKSEG----CNRSWPFNLEEIKDLLP-- 98

Query: 106 TQLEMYWPDVISG--DHETFWKHEWDKH 130
            ++  YWPDVI    +   FWKHEW+KH
Sbjct: 99  -EMRAYWPDVIHSFPNRSRFWKHEWEKH 118

[Close]
BLAST of Cp4.1LG09g04300 vs. TrEMBL
Match: A0A0A0LAB0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G736680 PE=3 SV=1)

HSP 1 Score: 137.1 bits (344), Expect = 2.5e-29
Identity = 79/203 (38.92%), Postives = 102/203 (50.25%), Query Frame = 1 

Query: 29  ISLLLFFNSVYSFDYFQMVLQWPPATCKTKDVVCYTAPPKMFTIHGLWPSYFSGDMVVCT 88
           +S LLF N +  FD+FQ+V QW P  C      CY  P  MFTIHGLWPS FS   ++C 
Sbjct: 11  LSSLLFVNGI-PFDFFQLVQQWGPNICSPAGSKCYAQPQPMFTIHGLWPSNFSTVPILCP 70

Query: 89  SKAKLDPTKQLAPLKTQLEMYWPDVISGDHETFWKHEWDKHA----------------IL 148
              KL P  ++  L+  L  YWPDVISG +  FW+HEW KHA                + 
Sbjct: 71  RTKKLFPQGKVTSLEQHLNKYWPDVISGKNSNFWEHEWRKHADCIDPPFTIFWYFDISLR 130

Query: 149 NAAHLNPDVNISLN----------YLVVENA---IKIAIGKRPALHCN-KDTSKQKQLFE 202
           +  +   D+   LN           L+ +N    IK A G  P + CN    +K+ QL E
Sbjct: 131 HRMNKTYDLLTILNDAGLSHQTHKDLISQNVLDPIKNATGMEPGIRCNINGITKKLQLKE 190

[Close]
BLAST of Cp4.1LG09g04300 vs. TrEMBL
Match: A0A0A0LDS5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G743990 PE=3 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 1.3e-25
Identity = 68/187 (36.36%), Postives = 93/187 (49.73%), Query Frame = 1 

Query: 41  FDYFQMVLQWPPATCKTKDVVCYTAPPKMFTIHGLWPSYFSGDMVVCTSKAKLDPTKQLA 100
           + +FQMV QW PA C +  V C+  P  MFTIHGLWPS F+  M+   S    D + Q+ 
Sbjct: 21  YQFFQMVQQWGPAKCSSGRVKCHVTPKPMFTIHGLWPSNFTDLMLHYCSLQSFDAS-QIK 80

Query: 101 PLKTQLEMYWPDVISGDHETFWKHEWDK-----------------------------HAI 160
            L+++L  YWPDV+ G    FWKHEW+K                              AI
Sbjct: 81  TLQSELSKYWPDVVKGKDVDFWKHEWEKHGTCSNPPFNIFQYFELALNIRKYKKYDLMAI 140

Query: 161 LNAAHLNPDVNISLNYLVVENAIKIAIGKRPALHCNKDTSK--QKQLFEIILCFDTDAVT 197
           LN A L+P  +    Y  + + I+ A+  +P L CN    +    QL+E+ILCFD   V 
Sbjct: 141 LNNAGLHPSTSKLHQYDDIADLIQAAVEAKPLLKCNDKNGQGQNNQLWEVILCFDHGGVN 200

[Close]
BLAST of Cp4.1LG09g04300 vs. TrEMBL
Match: A0A0A0LAN6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G745530 PE=3 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 6.2e-25
Identity = 74/210 (35.24%), Postives = 102/210 (48.57%), Query Frame = 1 

Query: 22  SNLLPICISLLLFFNSVYSFDYFQMVLQWPPATCKTKDVV--CYTAPPKMFTIHGLWPSY 81
           S L+   +SL++F N    F++FQ V QW P  C T   V  C+T P   FTIHGLWPS 
Sbjct: 4   SILIIYFLSLVVFVNGGVPFEFFQFVEQWGPNVCNTGARVSPCHTPPQAKFTIHGLWPSN 63

Query: 82  FSGDMVVCTSKAKLDPTKQLAPLKTQLEMYWPDVISGDHETFWKHEWDKH---------- 141
            S   ++C SK       ++  L+ QL + WPD+  G+++ FW HEWD H          
Sbjct: 64  HSNAHLLCQSKTIYS---KVISLEGQLNISWPDLKYGNNDKFWGHEWDTHGKCSDPPFSL 123

Query: 142 -----------------AILNAAHLNPDVNISLNYLVVENAIKIAIGKRPALHCNKDTSK 201
                             IL AA LNP  + +L    +   I+    K P + CNK+   
Sbjct: 124 FQYFQISLNLLRKFDLLTILKAAGLNPQTSQNLAIQNIMAPIQRTTKKYPGIRCNKNVKT 183

[Close]
BLAST of Cp4.1LG09g04300 vs. TrEMBL
Match: G3C8T8_9ROSA (S-locus associated ribonuclease OS=Prunus speciosa GN=S-RNase PE=3 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 1.2e-23
Identity = 76/219 (34.70%), Postives = 109/219 (49.77%), Query Frame = 1 

Query: 17  MAFIPSNLLPICISLLLFF---NSVYSFDYFQMVLQWPPATCKTKDVVCYTAPPKMFTIH 76
           MA + S+   + ++L+LF+    S  S+ YFQ V QWPP TC      CY  PP +FTIH
Sbjct: 1   MAMLKSSPAFLVLALVLFWCFSTSTGSYQYFQFVQQWPPTTCAISKKPCYQNPPSIFTIH 60

Query: 77  GLWPSYFSGDMVVCTSKAKLDPTK---QLAP-LKTQLEMYWPDVISGDHETFWKHEWDKH 136
           GLWPS +S +  V    A   PT+    L+P L+T+L++ WPDV SG++  FW+ EW+KH
Sbjct: 61  GLWPSNYSKNAWV----ANCSPTRFNNSLSPRLETKLKISWPDVESGNYTDFWEREWNKH 120

Query: 137 AILNAAHLNPD---------------VNI-----------SLNYLVVENAIKIAIGKRPA 196
              +   L+ +                NI             +Y  + + IK AI K PA
Sbjct: 121 GTCSEQTLDQEQYFERSHDIWNAYNITNILKRAKILPTGGKWDYSDIVSPIKTAIRKMPA 180

[Close]
BLAST of Cp4.1LG09g04300 vs. TrEMBL
Match: A0A067L9R7_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_15612 PE=3 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 4.5e-23
Identity = 71/193 (36.79%), Postives = 87/193 (45.08%), Query Frame = 1 

Query: 41  FDYFQMVLQWPPATCKTKDVVCYTA---PPKMFTIHGLWPSYFSGDMVVCTSKAKLDPTK 100
           FD+F  V QWP + C T+   CY     P   F IHGLWP+Y  G      +   +    
Sbjct: 26  FDFFYFVQQWPGSYCDTRHSCCYPKTGKPAADFGIHGLWPNYKDGGYPSNCNPDSVFDKS 85

Query: 101 QLAPLKTQLEMYWPDVI--SGDHETFWKHEWDKHA------------------------- 160
           Q++ L ++LE  WP +   SGD   FW HEW KH                          
Sbjct: 86  QISDLVSRLERNWPTLSCPSGDGNKFWSHEWVKHGTCAESELDQHDYFEAALKLKEKVNL 145

Query: 161 --ILNAAHLNPDVNISLNYLVVENAIKIAIGKRPALHCNKDTSKQKQLFEIILCFDTDAV 202
             IL  A + PD         +E AIK AIG  P L CNKD SK  QLF+I LC DT A 
Sbjct: 146 LKILTDAGIKPDDEF-YELETIEEAIKDAIGYTPGLECNKDGSKNTQLFQIYLCVDTSAS 205

[Close]
BLAST of Cp4.1LG09g04300 vs. TAIR10
Match: AT2G02990.1 (AT2G02990.1 ribonuclease 1)

HSP 1 Score: 104.4 bits (259), Expect = 8.9e-23
Identity = 68/210 (32.38%), Postives = 96/210 (45.71%), Query Frame = 1 

Query: 24  LLPIC-ISLLLFFNSVYS-------FDYFQMVLQWPPATCKTKDVVCYT---APPKMFTI 83
           L  +C ISLL+   SV+S       FD+F  V QWP + C T+   CY     P   F I
Sbjct: 5   LASLCLISLLVILPSVFSASSSSEDFDFFYFVQQWPGSYCDTQKKCCYPNSGKPAADFGI 64

Query: 84  HGLWPSYFSGDMVVCTSKAKLDPTKQLAPLKTQLEMYWPDVI--SGDHETFWKHEWDKHA 143
           HGLWP+Y  G        +K   +  ++ L T ++  WP +   SG  E FW+HEW+KH 
Sbjct: 65  HGLWPNYKDGTYPSNCDASKPFDSSTISDLLTSMKKSWPTLACPSGSGEAFWEHEWEKHG 124

Query: 144 I---------------------------LNAAHLNPDVNISLNYLVVENAIKIAIGKRPA 194
                                       L  A +NPD   S +   + ++IK +IG  P 
Sbjct: 125 TCSESVIDQHEYFQTALNLKQKTNLLGALTKAGINPDGK-SYSLESIRDSIKESIGFTPW 184

[Close]
BLAST of Cp4.1LG09g04300 vs. TAIR10
Match: AT1G26820.1 (AT1G26820.1 ribonuclease 3)

HSP 1 Score: 103.6 bits (257), Expect = 1.5e-22
Identity = 62/186 (33.33%), Postives = 84/186 (45.16%), Query Frame = 1 

Query: 41  FDYFQMVLQWPPATCKTKDVVCYTA---PPKMFTIHGLWPSYFSGDMVV-CTSKAKLDPT 100
           FD+F  VLQWP A C ++   CY     P   F IHGLWP+Y +G     C   ++ D  
Sbjct: 22  FDFFYFVLQWPGAYCDSRHSCCYPQTGKPAADFGIHGLWPNYKTGGWPQNCNPDSRFDDL 81

Query: 101 KQLAPLKTQLEMYWPDVI--SGDHETFWKHEWDKHAI----------------------- 160
           + ++ L + L+  WP +   S D   FW HEW+KH                         
Sbjct: 82  R-VSDLMSDLQREWPTLSCPSNDGMKFWTHEWEKHGTCAESELDQHDYFEAGLKLKQKAN 141

Query: 161 ----LNAAHLNPDVNISLNYLVVENAIKIAIGKRPALHCNKDTSKQKQLFEIILCFDTDA 194
               L  A + PD         +EN IK  +G  P + CNKD+S   QL++I LC DT A
Sbjct: 142 LLHALTNAGIKPDDKF-YEMKDIENTIKQVVGFAPGIECNKDSSHNSQLYQIYLCVDTSA 201

[Close]
BLAST of Cp4.1LG09g04300 vs. TAIR10
Match: AT1G14210.1 (AT1G14210.1 Ribonuclease T2 family protein)

HSP 1 Score: 86.7 bits (213), Expect = 1.9e-17
Identity = 62/218 (28.44%), Postives = 91/218 (41.74%), Query Frame = 1 

Query: 27  ICISLLLFFNSVYS-------FDYFQMVLQWPPATCKTKDVVCYTAPPKM------FTIH 86
           I +SLL+  + V S       F++F  V  WP A C ++   C   PP        F IH
Sbjct: 5   IIVSLLILQSLVVSSSQTEPDFNFFYWVNYWPGAICDSQKGCC---PPTKGNTASDFIIH 64

Query: 87  GLWPSYFSGDMVVCTSKAKLDPTKQLAPLKTQLEMYWPD----VISGDHETFWKHEWDKH 146
           GLWP + +G       +  L    +++ L  Q+E  W +        +    W+HEW+KH
Sbjct: 65  GLWPQFNNGTWPAFCDQTNLFDISKISDLVCQMEKKWTEWGVWACPSNETNLWEHEWNKH 124

Query: 147 AILNAA----HLNPDVNIS-------LNYLV---------------VENAIKIAIGKRPA 202
                +    H     N+        LN L+               ++NAIK AIG  P 
Sbjct: 125 GTCVQSIFDQHSYFRTNLKFKHKVHLLNILIQKGIKPNDGFYSLDEIKNAIKCAIGFAPG 184

[Close]
BLAST of Cp4.1LG09g04300 vs. NCBI nr
Match: gi|778683692|ref|XP_011651927.1| (PREDICTED: ribonuclease MC-like [Cucumis sativus])

HSP 1 Score: 137.1 bits (344), Expect = 3.5e-29
Identity = 79/203 (38.92%), Postives = 102/203 (50.25%), Query Frame = 1 

Query: 29  ISLLLFFNSVYSFDYFQMVLQWPPATCKTKDVVCYTAPPKMFTIHGLWPSYFSGDMVVCT 88
           +S LLF N +  FD+FQ+V QW P  C      CY  P  MFTIHGLWPS FS   ++C 
Sbjct: 11  LSSLLFVNGI-PFDFFQLVQQWGPNICSPAGSKCYAQPQPMFTIHGLWPSNFSTVPILCP 70

Query: 89  SKAKLDPTKQLAPLKTQLEMYWPDVISGDHETFWKHEWDKHA----------------IL 148
              KL P  ++  L+  L  YWPDVISG +  FW+HEW KHA                + 
Sbjct: 71  RTKKLFPQGKVTSLEQHLNKYWPDVISGKNSNFWEHEWRKHADCIDPPFTIFWYFDISLR 130

Query: 149 NAAHLNPDVNISLN----------YLVVENA---IKIAIGKRPALHCN-KDTSKQKQLFE 202
           +  +   D+   LN           L+ +N    IK A G  P + CN    +K+ QL E
Sbjct: 131 HRMNKTYDLLTILNDAGLSHQTHKDLISQNVLDPIKNATGMEPGIRCNINGITKKLQLKE 190

[Close]
BLAST of Cp4.1LG09g04300 vs. NCBI nr
Match: gi|659085730|ref|XP_008443571.1| (PREDICTED: ribonuclease MC-like [Cucumis melo])

HSP 1 Score: 127.9 bits (320), Expect = 2.1e-26
Identity = 77/205 (37.56%), Postives = 103/205 (50.24%), Query Frame = 1 

Query: 27  ICISLLLFFNSVYSFDYFQMVLQWPPATCKTKDVVCYTAPPKMFTIHGLWPSYFSGDMVV 86
           +C+  LL F + Y FD+FQ+V QW P TC      C+  P   FTIHGLWPS FS   + 
Sbjct: 8   LCLLSLLLFVNGYPFDFFQLVEQWGPNTCND-GTQCHAQPQPEFTIHGLWPSNFSNARLF 67

Query: 87  CTSKAKLDPTKQLAPLKTQLEMYWPDVISGDHETFWKHEWDKHA---------------- 146
           C ++ KL    Q++ L+  L+ YWPDVI+G++  FW +EW KH                 
Sbjct: 68  CATR-KLFDNGQISLLEGDLDRYWPDVINGNNRGFWSNEWKKHGACIDPPFNITQYFQLS 127

Query: 147 -----------ILNAA-HLNPDV-NISLNYLVVENAIKIAIGKRPALHCNKD-TSKQKQL 202
                      ILN   H N  + N  L    + N I  A  K   + CNK+  +K+ QL
Sbjct: 128 LDHHRTYDLLKILNGVWHRNRQMPNNGLAPKDILNPIFNATNKTAGIRCNKNGITKKLQL 187

[Close]
BLAST of Cp4.1LG09g04300 vs. NCBI nr
Match: gi|700203881|gb|KGN59014.1| (hypothetical protein Csa_3G743990 [Cucumis sativus])

HSP 1 Score: 124.8 bits (312), Expect = 1.8e-25
Identity = 68/187 (36.36%), Postives = 93/187 (49.73%), Query Frame = 1 

Query: 41  FDYFQMVLQWPPATCKTKDVVCYTAPPKMFTIHGLWPSYFSGDMVVCTSKAKLDPTKQLA 100
           + +FQMV QW PA C +  V C+  P  MFTIHGLWPS F+  M+   S    D + Q+ 
Sbjct: 21  YQFFQMVQQWGPAKCSSGRVKCHVTPKPMFTIHGLWPSNFTDLMLHYCSLQSFDAS-QIK 80

Query: 101 PLKTQLEMYWPDVISGDHETFWKHEWDK-----------------------------HAI 160
            L+++L  YWPDV+ G    FWKHEW+K                              AI
Sbjct: 81  TLQSELSKYWPDVVKGKDVDFWKHEWEKHGTCSNPPFNIFQYFELALNIRKYKKYDLMAI 140

Query: 161 LNAAHLNPDVNISLNYLVVENAIKIAIGKRPALHCNKDTSK--QKQLFEIILCFDTDAVT 197
           LN A L+P  +    Y  + + I+ A+  +P L CN    +    QL+E+ILCFD   V 
Sbjct: 141 LNNAGLHPSTSKLHQYDDIADLIQAAVEAKPLLKCNDKNGQGQNNQLWEVILCFDHGGVN 200

[Close]
BLAST of Cp4.1LG09g04300 vs. NCBI nr
Match: gi|700203887|gb|KGN59020.1| (hypothetical protein Csa_3G745530 [Cucumis sativus])

HSP 1 Score: 122.5 bits (306), Expect = 9.0e-25
Identity = 74/210 (35.24%), Postives = 102/210 (48.57%), Query Frame = 1 

Query: 22  SNLLPICISLLLFFNSVYSFDYFQMVLQWPPATCKTKDVV--CYTAPPKMFTIHGLWPSY 81
           S L+   +SL++F N    F++FQ V QW P  C T   V  C+T P   FTIHGLWPS 
Sbjct: 4   SILIIYFLSLVVFVNGGVPFEFFQFVEQWGPNVCNTGARVSPCHTPPQAKFTIHGLWPSN 63

Query: 82  FSGDMVVCTSKAKLDPTKQLAPLKTQLEMYWPDVISGDHETFWKHEWDKH---------- 141
            S   ++C SK       ++  L+ QL + WPD+  G+++ FW HEWD H          
Sbjct: 64  HSNAHLLCQSKTIYS---KVISLEGQLNISWPDLKYGNNDKFWGHEWDTHGKCSDPPFSL 123

Query: 142 -----------------AILNAAHLNPDVNISLNYLVVENAIKIAIGKRPALHCNKDTSK 201
                             IL AA LNP  + +L    +   I+    K P + CNK+   
Sbjct: 124 FQYFQISLNLLRKFDLLTILKAAGLNPQTSQNLAIQNIMAPIQRTTKKYPGIRCNKNVKT 183

[Close]
BLAST of Cp4.1LG09g04300 vs. NCBI nr
Match: gi|326535671|gb|ADZ76512.1| (S-locus associated ribonuclease [Prunus speciosa])

HSP 1 Score: 118.2 bits (295), Expect = 1.7e-23
Identity = 76/219 (34.70%), Postives = 109/219 (49.77%), Query Frame = 1 

Query: 17  MAFIPSNLLPICISLLLFF---NSVYSFDYFQMVLQWPPATCKTKDVVCYTAPPKMFTIH 76
           MA + S+   + ++L+LF+    S  S+ YFQ V QWPP TC      CY  PP +FTIH
Sbjct: 1   MAMLKSSPAFLVLALVLFWCFSTSTGSYQYFQFVQQWPPTTCAISKKPCYQNPPSIFTIH 60

Query: 77  GLWPSYFSGDMVVCTSKAKLDPTK---QLAP-LKTQLEMYWPDVISGDHETFWKHEWDKH 136
           GLWPS +S +  V    A   PT+    L+P L+T+L++ WPDV SG++  FW+ EW+KH
Sbjct: 61  GLWPSNYSKNAWV----ANCSPTRFNNSLSPRLETKLKISWPDVESGNYTDFWEREWNKH 120

Query: 137 AILNAAHLNPD---------------VNI-----------SLNYLVVENAIKIAIGKRPA 196
              +   L+ +                NI             +Y  + + IK AI K PA
Sbjct: 121 GTCSEQTLDQEQYFERSHDIWNAYNITNILKRAKILPTGGKWDYSDIVSPIKTAIRKMPA 180

The following BLAST results are available for this feature:
BLAST of Cp4.1LG09g04300 vs. Swiss-Prot
Match NameE-valueIdentityDescription
RNLE_SOLLC8.4e-2331.82Extracellular ribonuclease LE OS=Solanum lycopersicum PE=1 SV=2[more]
RNS1_ARATH1.6e-2132.38Ribonuclease 1 OS=Arabidopsis thaliana GN=RNS1 PE=1 SV=1[more]
RNS3_ARATH2.7e-2133.33Ribonuclease 3 OS=Arabidopsis thaliana GN=RNS3 PE=2 SV=1[more]
RNS2_ANTHI7.4e-1130.73Ribonuclease S-2 OS=Antirrhinum hispanicum GN=S2 PE=2 SV=1[more]
RNT2_HUMAN1.8e-0939.77Ribonuclease T2 OS=Homo sapiens GN=RNASET2 PE=1 SV=2[more]
BLAST of Cp4.1LG09g04300 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LAB0_CUCSA2.5e-2938.92Uncharacterized protein OS=Cucumis sativus GN=Csa_3G736680 PE=3 SV=1[more]
A0A0A0LDS5_CUCSA1.3e-2536.36Uncharacterized protein OS=Cucumis sativus GN=Csa_3G743990 PE=3 SV=1[more]
A0A0A0LAN6_CUCSA6.2e-2535.24Uncharacterized protein OS=Cucumis sativus GN=Csa_3G745530 PE=3 SV=1[more]
G3C8T8_9ROSA1.2e-2334.70S-locus associated ribonuclease OS=Prunus speciosa GN=S-RNase PE=3 SV=1[more]
A0A067L9R7_JATCU4.5e-2336.79Uncharacterized protein OS=Jatropha curcas GN=JCGZ_15612 PE=3 SV=1[more]
BLAST of Cp4.1LG09g04300 vs. TAIR10
Match NameE-valueIdentityDescription
AT2G02990.18.9e-2332.38 ribonuclease 1[more]
AT1G26820.11.5e-2233.33 ribonuclease 3[more]
AT1G14210.11.9e-1728.44 Ribonuclease T2 family protein[more]
BLAST of Cp4.1LG09g04300 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|778683692|ref|XP_011651927.1|3.5e-2938.92PREDICTED: ribonuclease MC-like [Cucumis sativus][more]
gi|659085730|ref|XP_008443571.1|2.1e-2637.56PREDICTED: ribonuclease MC-like [Cucumis melo][more]
gi|700203881|gb|KGN59014.1|1.8e-2536.36hypothetical protein Csa_3G743990 [Cucumis sativus][more]
gi|700203887|gb|KGN59020.1|9.0e-2535.24hypothetical protein Csa_3G745530 [Cucumis sativus][more]
gi|326535671|gb|ADZ76512.1|1.7e-2334.70S-locus associated ribonuclease [Prunus speciosa][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0033897ribonuclease T2 activity
GO:0003723RNA binding
Vocabulary: INTERPRO
TermDefinition
IPR018188RNase_T2_His_AS_1
IPR001568RNase_T2-like
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0033897 ribonuclease T2 activity
molecular_function GO:0003723 RNA binding
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG09g04300.1Cp4.1LG09g04300.1mRNA


InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001568Ribonuclease T2-likeGENE3DG3DSA:3.90.730.10coord: 41..130
score: 4.5E-28coord: 131..202
score: 6.3
IPR001568Ribonuclease T2-likePANTHERPTHR11240RIBONUCLEASE T2coord: 1..193
score: 6.4
IPR001568Ribonuclease T2-likePFAMPF00445Ribonuclease_T2coord: 131..193
score: 2.1E-7coord: 43..130
score: 5.3
IPR001568Ribonuclease T2-likeunknownSSF55895Ribonuclease Rh-likecoord: 37..201
score: 2.22
IPR018188Ribonuclease T2, His active site 1PROSITEPS00530RNASE_T2_1coord: 70..77
scor

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Cp4.1LG09g04300CmaCh18G008740Cucurbita maxima (Rimu)cmacpeB415
Cp4.1LG09g04300CmoCh18G008790Cucurbita moschata (Rifu)cmocpeB376
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Cp4.1LG09g04300Cucumber (Gy14) v2cgybcpeB581
Cp4.1LG09g04300Melon (DHL92) v3.6.1cpemedB028
Cp4.1LG09g04300Silver-seed gourdcarcpeB0677
Cp4.1LG09g04300Silver-seed gourdcarcpeB1169
Cp4.1LG09g04300Cucumber (Chinese Long) v3cpecucB0043
Cp4.1LG09g04300Wax gourdcpewgoB0055
Cp4.1LG09g04300Cucurbita pepo (Zucchini)cpecpeB040
Cp4.1LG09g04300Cucumber (Gy14) v1cgycpeB0804
Cp4.1LG09g04300Cucurbita maxima (Rimu)cmacpeB700
Cp4.1LG09g04300Cucurbita moschata (Rifu)cmocpeB651
Cp4.1LG09g04300Wild cucumber (PI 183967)cpecpiB036
Cp4.1LG09g04300Cucumber (Chinese Long) v2cpecuB041
Cp4.1LG09g04300Bottle gourd (USVL1VR-Ls)cpelsiB032
Cp4.1LG09g04300Melon (DHL92) v3.5.1cpemeB024