Cp4.1LG08g08500 (gene) Cucurbita pepo (Zucchini)

NameCp4.1LG08g08500
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
DescriptionTHO complex subunit 3
LocationCp4.1LG08 : 6711543 .. 6715352 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTGAATTCTTGAATTAATCCCCCAAATAAAAAGTGGATGTTCGCATGGAACAAAAATCCCTATAATCGCCGGATTGGAACTCGTTGAATTCCAGGCGGACATGGAGGAATCGACGCCGGCTTTTAAGAATCTTTACAGCAGAGAGTATCAAGGTCACAAGAAGAAGGTTTTTATCAAATCGCTTCCTTCGTGCTGTTGTTCTCTGTGGCGATTTCATTTTTCGTTTTGATCTTAAATTCATCTTTTCTTGTAACTGCGAAAGGTACATTCTGTGGCATGGAATTGCACTGGTATGAAGCTTGCTTCCGGTTCTGTCGATCAAACTGCTCGAGTTTGGCATATTGAGCCTCATGGACATGTATGCCTTCTATCACACTTGTTTTTTTTTTTTGTTCTAGGGTCTTCCCAATTCATTGTTTCTGTTGTGGACTCTTGAATCTTGTTCTCAAATTGATTGAATAGGGTTTTTTTTTTTCCTTCCTTTTTTTGTTTTGGGTACTGTAAGTAGTTGATTCGAGTTGATTCGAGCTTAAGTGTTATATTGAATTCTTCTATTCTGTTCTCCTTATGGGTTTTCTTTTCCTACGCCTGATATGATAACATATACACATTTATCTGTTTCCCAGGTAATGTTTTGGAGTTTCTACTACCTAGCACGAATGCTAACATTTCCTATCTCTTGCTCAACTTTTCTAGGCTGATAAAGTTTAGCTGCATTTCCTATAGTTGATTATGTGAATGAAGTTATGGAAAGAACATTAATATTTGTATATATTTGTGTGTATATAGATATATGCACATAAACTGGGTATGTGAGCTCCATGCTCATGGCCTACCTTCGAGGTTTTGCCCACTACAAGGCCAATTTCGAGGGATAACAAGATATTTAGTTATTAAGGAAATAGAATAAGCTAATGATCTTCATGGAACGGGATATGTTCTATATGTTGTTCGACCGTATCCTTGAGTTCTATAAAAAGGAGAGAGAATGATCCTTCTTGGCTCCGAATTGTATTAGTAGACTAGTTAAGTTTGGGAAAGCTTCGATAGTAATTGTATTAGTAGACTAATTAGTGTTTCATAAGCTCTTTCATTTTCCTTATTTTTAAGTTCTGGAAAAAGCGGAGTGCAATTTGCTTAGTTCTCTCGTCGTGTATACTCGTGAGAGTGTGTTGCGTTTGCTACCTCCCAAGACCTGTTTTGAATGAATTCTATGATTTTGCTATGGTGCTGTTCCACCACAGGGTTTAGTGTTGTCTTGTTTGTATCTATTTACTTTCAACCCTGAACAAATCATTTCAATCATGTCAGATGATGTTTGCTTCAGGGTAAGGTTAAGGATGTCGAGTTGAAAGGGCATACTGATAGTGTAGATCAGCTATGTTGGGATCCTAAACATTCTGATCTTATAGCAACCGCCTCTGGTGACAAGACTGTTCGACTATGGGATGCTCGTAGTAAGTGTTTGAAACTGAGATTTGTGAAGTAACTGATGTAATTGATGTTTAGAATATGGCTCATAATTTCTGATTCTGTTCCATAGATGGGAAATGCTCACAGCAAGCTGAGCTGAGTGGGGAGAATATAAACATCACCTACAAGCCTGATGGGACGCACATAGCTGTTGGGAATAGGGTATGTTCTAGAGTGAAATTTGTGCATCATAAAACCAATTTAGATTTGTTATTGCTTTAATGTACACTGCAAGGTGTTTATATCCCTGTGCAACTACTTGACTAATTTAGATTTGTCATTTTGCAGGATGATGAACTCACTATACTGGATGTTAGGAAGTTTAAGCCAGTTCACAAGCGCAAGTTCAATTACGAGGTAACGTTTATGTTACAATCAACAACAATGACTCGAGCATTGGTCATTTATGACAACTTAAAAAGAGGATTGTGTATTAAGAAATTGAACAAATCACAACAGGACATAATAGCATGCATATGACTGCTTTGACATGTTGATGATCGTCTTCTTACCTTTAAAATTGTAAGTCCAATTGTATTAGAGGCTGTTTTTGCTCTGTTCTTGTAATAGTGTGATGTAATATGGACCATTTGTCGGGTTAATTAGTAACTAGTACAGGTGGGAATTTATAGTAGTATATGCCAAGTCTTATGTGAAGGGAGAAGACGTGCCTAGCTTGAGTTTGATATCCTAGTGCGTATAGGGAGCTTTCTAAATAACCCTTAAGCTTTCTCAATACTCTTGAAATGTAGTAGATTCTAATTATATCAACCCTTTCGTATCTACCGCCTTTCTTTCCAGTTTCAGTTCGTTTTTTTTTTCCTTCTCTTTTGCTATTGGCAGACATGTTTGTTTTACTTTGATTATCTATATTGACATAATTTTCTATTATCTTTTCCAGGTGAACGAAATTGCTTGGAACATGACTGGGGAAATGTTTTTCCTGACAACTGGAAATGGTGATGATCTGCAGCTATGACCCTTTCTTTTCATGCCACGACTGAGGAGATTTTGTTAATTTCACTCTATTGAATAAATTTTCATGTCTACTTCTTGTGCTCAGGTACCGTTGAAGTACTAGCATACCCGTCACTTCGACCTATTGAAACTCTTATGGCTCACACAGCTGGTTGTTACTGCATCGCAATTGACCCGGTTGGAGGGTGAGATTCAAAGTTGACCTTAAGCGTATCTTCCACGATAAAAGAACTGTGTACAACTTTCACATGCAATATGCAGTCATGATTTCTTCTATTAGAAAGATCGTCGGTTTCATTCTTTCAATTGCTTGTTTCGATCGTTTAGAAAATTGCTTCTGCAATTAGCCATGGGGAAAGATAAATTAATTCCTTGTTGCATCATAGGTATTTTGCAGTTGGAAGTGCTGATTCATTAGTTAGCCTATGGGATATCTCTCAGATGCTCTGCGTGCGAACATTTACAAAACTCGAGTAAGTTTTTTGTTTGGTTTATACAATGATTTTGTTTTCTTCTTGTCTCAATCCATTTCTATCCTGTCAATCGAAACACATCGGGAGCTTTGTGGCTTGGGTTACGACACTGCATTATGTAGCGACTTTTTTGAACACTGAAGAATGTATTTTCATGTCACTTCTTTTACTTCCTTAGATGGCCTGTCCGAACTATAAGTTTCAACCACACAGGAGAATACATTGCTTCTGCTAGTGAGGACTTGTTCATTGATATAGTAAGCATTCTAGCGCCTCTCCTTTCTTTAAATATCCGTTCTATGAAGGCGTAGAATCCCCCCTTCCTTGAGGATTAGTGGCAAAACTGGTTCATGAAACGGTGTTTTTCTTTTGCGTACAGTCGAGTGTTCAATCGGGACGAACGGTTCATCAGATTCCTTGTCGGGCTGCTATGAATAGTGTGGAGTGGAATCCAAAGCACAATTTGCTTGCATATGCTGGGGATGACAAGAATAAGTACCAGGCTGATGAAGGCAAGTTATCTATGCGTCGATATACCCCACTGATCTTTTTAATTCAATGCATTCCTCCTAGGTTTTAGTTTCAAGACTTCACAAGTGTTATCTTTATTTTGTTATTGCAGGTATTTTTAGGATCTTTGGGTTTGAAAGTGCATGAGAAATGGATCGACTAAGATTCTCCATAGAATTCTACCATGGATCAACTTATCTTTATTTCCTTCTTTTCTGGTTAGTATTAGTATTGAATTCCATTTGTATGCTATGTTACTCAGGATTTTCCTTCTTTTCGCTTGGAAGGTTCAAAGTACTTTTGTTTTTGTGATCTGAAAAACAATCATTTGAGCTAGCGATGAAAGGTTCAAAGTACTTTTGTGATCTGAAAATG

mRNA sequence

ATTGAATTCTTGAATTAATCCCCCAAATAAAAAGTGGATGTTCGCATGGAACAAAAATCCCTATAATCGCCGGATTGGAACTCGTTGAATTCCAGGCGGACATGGAGGAATCGACGCCGGCTTTTAAGAATCTTTACAGCAGAGAGTATCAAGGTCACAAGAAGAAGGTACATTCTGTGGCATGGAATTGCACTGGTATGAAGCTTGCTTCCGGTTCTGTCGATCAAACTGCTCGAGTTTGGCATATTGAGCCTCATGGACATGGTAAGGTTAAGGATGTCGAGTTGAAAGGGCATACTGATAGTGTAGATCAGCTATGTTGGGATCCTAAACATTCTGATCTTATAGCAACCGCCTCTGGTGACAAGACTGTTCGACTATGGGATGCTCGTAATGGGAAATGCTCACAGCAAGCTGAGCTGAGTGGGGAGAATATAAACATCACCTACAAGCCTGATGGGACGCACATAGCTGTTGGGAATAGGGATGATGAACTCACTATACTGGATGTTAGGAAGTTTAAGCCAGTTCACAAGCGCAAGTTCAATTACGAGGTGAACGAAATTGCTTGGAACATGACTGGGGAAATGTTTTTCCTGACAACTGGAAATGGTACCGTTGAAGTACTAGCATACCCGTCACTTCGACCTATTGAAACTCTTATGGCTCACACAGCTGGTTGTTACTGCATCGCAATTGACCCGGTTGGAGGGTATTTTGCAGTTGGAAGTGCTGATTCATTAGTTAGCCTATGGGATATCTCTCAGATGCTCTGCGTGCGAACATTTACAAAACTCGAATGGCCTGTCCGAACTATAAGTTTCAACCACACAGGAGAATACATTGCTTCTGCTAGTGAGGACTTGTTCATTGATATATCGAGTGTTCAATCGGGACGAACGGTTCATCAGATTCCTTGTCGGGCTGCTATGAATAGTGTGGAGTGGAATCCAAAGCACAATTTGCTTGCATATGCTGGGGATGACAAGAATAAGTACCAGGCTGATGAAGGCAAGTTATCTATGCGTATTTTTAGGATCTTTGGGTTTGAAAGTGCATGAGAAATGGATCGACTAAGATTCTCCATAGAATTCTACCATGGATCAACTTATCTTTATTTCCTTCTTTTCTGGTTAGTATTAGTATTGAATTCCATTTGTATGCTATGTTACTCAGGATTTTCCTTCTTTTCGCTTGGAAGGTTCAAAGTACTTTTGTGATCTGAAAATG

Coding sequence (CDS)

ATGGAGGAATCGACGCCGGCTTTTAAGAATCTTTACAGCAGAGAGTATCAAGGTCACAAGAAGAAGGTACATTCTGTGGCATGGAATTGCACTGGTATGAAGCTTGCTTCCGGTTCTGTCGATCAAACTGCTCGAGTTTGGCATATTGAGCCTCATGGACATGGTAAGGTTAAGGATGTCGAGTTGAAAGGGCATACTGATAGTGTAGATCAGCTATGTTGGGATCCTAAACATTCTGATCTTATAGCAACCGCCTCTGGTGACAAGACTGTTCGACTATGGGATGCTCGTAATGGGAAATGCTCACAGCAAGCTGAGCTGAGTGGGGAGAATATAAACATCACCTACAAGCCTGATGGGACGCACATAGCTGTTGGGAATAGGGATGATGAACTCACTATACTGGATGTTAGGAAGTTTAAGCCAGTTCACAAGCGCAAGTTCAATTACGAGGTGAACGAAATTGCTTGGAACATGACTGGGGAAATGTTTTTCCTGACAACTGGAAATGGTACCGTTGAAGTACTAGCATACCCGTCACTTCGACCTATTGAAACTCTTATGGCTCACACAGCTGGTTGTTACTGCATCGCAATTGACCCGGTTGGAGGGTATTTTGCAGTTGGAAGTGCTGATTCATTAGTTAGCCTATGGGATATCTCTCAGATGCTCTGCGTGCGAACATTTACAAAACTCGAATGGCCTGTCCGAACTATAAGTTTCAACCACACAGGAGAATACATTGCTTCTGCTAGTGAGGACTTGTTCATTGATATATCGAGTGTTCAATCGGGACGAACGGTTCATCAGATTCCTTGTCGGGCTGCTATGAATAGTGTGGAGTGGAATCCAAAGCACAATTTGCTTGCATATGCTGGGGATGACAAGAATAAGTACCAGGCTGATGAAGGCAAGTTATCTATGCGTATTTTTAGGATCTTTGGGTTTGAAAGTGCATGA

Protein sequence

MEESTPAFKNLYSREYQGHKKKVHSVAWNCTGMKLASGSVDQTARVWHIEPHGHGKVKDVELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDGTHIAVGNRDDELTILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPSLRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTISFNHTGEYIASASEDLFIDISSVQSGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQADEGKLSMRIFRIFGFESA
BLAST of Cp4.1LG08g08500 vs. Swiss-Prot
Match: THOC3_ARATH (THO complex subunit 3 OS=Arabidopsis thaliana GN=THO3 PE=1 SV=1)

HSP 1 Score: 575.9 bits (1483), Expect = 2.8e-163
Identity = 270/320 (84.38%), Postives = 297/320 (92.81%), Query Frame = 1

Query: 1   MEESTPAFKNLYSREYQGHKKKVHSVAWNCTGMKLASGSVDQTARVWHIEPHGHGKVKDV 60
           MEE+T  FK+L+SREYQGHKKKVHSVAWN  G KLASGSVDQTAR+W+IEPHGH K KD+
Sbjct: 1   MEETTIPFKSLHSREYQGHKKKVHSVAWNSNGTKLASGSVDQTARIWNIEPHGHSKAKDL 60

Query: 61  ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDG 120
           ELKGHTDSVDQLCWDPKHSDL+ATASGDK+VRLWDAR+GKC+QQ ELSGENINITYKPDG
Sbjct: 61  ELKGHTDSVDQLCWDPKHSDLVATASGDKSVRLWDARSGKCTQQVELSGENINITYKPDG 120

Query: 121 THIAVGNRDDELTILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS 180
           TH+AVGNRDDELTILDVRKFKP+H+RKFNYEVNEIAWNM G+ FFLTTG GTVEVL+YPS
Sbjct: 121 THVAVGNRDDELTILDVRKFKPLHRRKFNYEVNEIAWNMPGDFFFLTTGLGTVEVLSYPS 180

Query: 181 LRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS 240
           L+P++TL AHTAGCYCIAIDP G YFAVGSADSLVSLWDIS MLC+RTFTKLEWPVRTIS
Sbjct: 181 LKPLDTLTAHTAGCYCIAIDPKGRYFAVGSADSLVSLWDISDMLCLRTFTKLEWPVRTIS 240

Query: 241 FNHTGEYIASASEDLFIDISSVQSGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKN-KY 300
           FN++GEYIASASEDLFIDI++VQ+GRTVHQIPCRAAMNSVEWNPK+NLLAYAGDDKN KY
Sbjct: 241 FNYSGEYIASASEDLFIDIANVQTGRTVHQIPCRAAMNSVEWNPKYNLLAYAGDDKNPKY 300

Query: 301 QADEGKLSMRIFRIFGFESA 320
             DEG     +FRIFGFES+
Sbjct: 301 NTDEG-----VFRIFGFESS 315

BLAST of Cp4.1LG08g08500 vs. Swiss-Prot
Match: THOC3_MOUSE (THO complex subunit 3 OS=Mus musculus GN=Thoc3 PE=2 SV=1)

HSP 1 Score: 335.5 bits (859), Expect = 6.4e-91
Identity = 151/300 (50.33%), Postives = 211/300 (70.33%), Query Frame = 1

Query: 13  SREYQGHKKKVHSVAWNCTGMKLASGSVDQTARVWHIEPHGHGKVKDVELKGHTDSVDQL 72
           +RE+  H  KVHSVAW+C G +LASGS D+TA V+ +E      VK+   +GH DSVDQL
Sbjct: 48  TREFPAHSAKVHSVAWSCDGRRLASGSFDKTASVFLLEKDR--LVKENNYRGHGDSVDQL 107

Query: 73  CWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDGTHIAVGNRDDEL 132
           CW P + DL  TASGDKT+R+WD R  KC       GENINI + PDG  IAVGN+DD +
Sbjct: 108 CWHPSNPDLFVTASGDKTIRIWDVRTTKCIATVNTKGENINICWSPDGQTIAVGNKDDVV 167

Query: 133 TILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPSLRPIETLMAHTA 192
           T +D +  +   + +F +EVNEI+WN    MFFLT GNG + +L+YP L+P++++ AH +
Sbjct: 168 TFIDAKTHRSKAEEQFKFEVNEISWNNDNNMFFLTNGNGCINILSYPELKPVQSINAHPS 227

Query: 193 GCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTISFNHTGEYIASAS 252
            C CI  DP+G YFA GSAD+LVSLWD+ +++CVR F++L+WPVRT+SF+H G+ +ASAS
Sbjct: 228 NCICIKFDPMGKYFATGSADALVSLWDVDELVCVRCFSRLDWPVRTLSFSHDGKMLASAS 287

Query: 253 EDLFIDISSVQSGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKN-KYQADEGKLSMRIF 312
           ED FIDI+ V++G  + ++ C +   +V W+PK  LLA+A DDK+ KY +     ++++F
Sbjct: 288 EDHFIDIAEVETGDKLWEVQCESPTFTVAWHPKRPLLAFACDDKDGKYDSSREAGTVKLF 345

BLAST of Cp4.1LG08g08500 vs. Swiss-Prot
Match: THOC3_BOVIN (THO complex subunit 3 OS=Bos taurus GN=THOC3 PE=2 SV=1)

HSP 1 Score: 335.5 bits (859), Expect = 6.4e-91
Identity = 151/300 (50.33%), Postives = 211/300 (70.33%), Query Frame = 1

Query: 13  SREYQGHKKKVHSVAWNCTGMKLASGSVDQTARVWHIEPHGHGKVKDVELKGHTDSVDQL 72
           +RE+  H  KVHSVAW+C G +LASGS D+TA V+ +E      VK+   +GH DSVDQL
Sbjct: 48  TREFPAHSAKVHSVAWSCDGRRLASGSFDKTASVFLLEKDR--LVKENNYRGHGDSVDQL 107

Query: 73  CWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDGTHIAVGNRDDEL 132
           CW P + DL  TASGDKT+R+WD R  KC       GENINI + PDG  IAVGN+DD +
Sbjct: 108 CWHPSNPDLFVTASGDKTIRIWDVRTTKCIATVNTKGENINICWSPDGQTIAVGNKDDVV 167

Query: 133 TILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPSLRPIETLMAHTA 192
           T +D +  +   + +F +EVNEI+WN    MFFLT GNG + +L+YP L+P++++ AH +
Sbjct: 168 TFIDAKTHRSKAEEQFKFEVNEISWNNDNNMFFLTNGNGCINILSYPELKPVQSINAHPS 227

Query: 193 GCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTISFNHTGEYIASAS 252
            C CI  DP+G YFA GSAD+LVSLWD+ +++CVR F++L+WPVRT+SF+H G+ +ASAS
Sbjct: 228 NCICIKFDPMGKYFATGSADALVSLWDVDELVCVRCFSRLDWPVRTLSFSHDGKMLASAS 287

Query: 253 EDLFIDISSVQSGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKN-KYQADEGKLSMRIF 312
           ED FIDI+ V++G  + ++ C +   +V W+PK  LLA+A DDK+ KY +     ++++F
Sbjct: 288 EDHFIDIAEVETGDKLWEVQCESPTFTVAWHPKRPLLAFACDDKDGKYDSSREAGTVKLF 345

BLAST of Cp4.1LG08g08500 vs. Swiss-Prot
Match: THOC3_HUMAN (THO complex subunit 3 OS=Homo sapiens GN=THOC3 PE=1 SV=1)

HSP 1 Score: 335.1 bits (858), Expect = 8.3e-91
Identity = 151/300 (50.33%), Postives = 211/300 (70.33%), Query Frame = 1

Query: 13  SREYQGHKKKVHSVAWNCTGMKLASGSVDQTARVWHIEPHGHGKVKDVELKGHTDSVDQL 72
           +RE+  H  KVHSVAW+C G +LASGS D+TA V+ +E      VK+   +GH DSVDQL
Sbjct: 48  TREFLAHSAKVHSVAWSCDGRRLASGSFDKTASVFLLEKDR--LVKENNYRGHGDSVDQL 107

Query: 73  CWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDGTHIAVGNRDDEL 132
           CW P + DL  TASGDKT+R+WD R  KC       GENINI + PDG  IAVGN+DD +
Sbjct: 108 CWHPSNPDLFVTASGDKTIRIWDVRTTKCIATVNTKGENINICWSPDGQTIAVGNKDDVV 167

Query: 133 TILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPSLRPIETLMAHTA 192
           T +D +  +   + +F +EVNEI+WN    MFFLT GNG + +L+YP L+P++++ AH +
Sbjct: 168 TFIDAKTHRSKAEEQFKFEVNEISWNNDNNMFFLTNGNGCINILSYPELKPVQSINAHPS 227

Query: 193 GCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTISFNHTGEYIASAS 252
            C CI  DP+G YFA GSAD+LVSLWD+ +++CVR F++L+WPVRT+SF+H G+ +ASAS
Sbjct: 228 NCICIKFDPMGKYFATGSADALVSLWDVDELVCVRCFSRLDWPVRTLSFSHDGKMLASAS 287

Query: 253 EDLFIDISSVQSGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKN-KYQADEGKLSMRIF 312
           ED FIDI+ V++G  + ++ C +   +V W+PK  LLA+A DDK+ KY +     ++++F
Sbjct: 288 EDHFIDIAEVETGDKLWEVQCESPTFTVAWHPKRPLLAFACDDKDGKYDSSREAGTVKLF 345

BLAST of Cp4.1LG08g08500 vs. Swiss-Prot
Match: AAC3_DICDI (WD repeat-containing protein AAC3 OS=Dictyostelium discoideum GN=AAC3 PE=2 SV=2)

HSP 1 Score: 241.1 bits (614), Expect = 1.6e-62
Identity = 120/334 (35.93%), Postives = 192/334 (57.49%), Query Frame = 1

Query: 8   FKNLYSREYQGHKKKVHSVAWNCTGMKLASGSVDQTARVWHIEPHGHGK----------- 67
           F    ++++ G+KKK  SVAWN  G K+AS   D   RVW+ +P G+             
Sbjct: 153 FSECSTKDFIGNKKKSTSVAWNANGTKIASSGSDGIVRVWNFDPLGNSNNNNNSNNTSSN 212

Query: 68  -----VKD-VELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGE 127
                +K+ +ELKGH  S++++ W PK++DL+A+A  DK +++WD + GKC      + E
Sbjct: 213 SKNNNIKETIELKGHDGSIEKISWSPKNNDLLASAGTDKVIKIWDVKIGKCIGTVSTNSE 272

Query: 128 NINITYKPDGTHIAVGNRDDELTILDVRKFKPVHKRKFN-YEVNEIAWNMTGEMFFLTTG 187
           NI++ + PDG  I    RDD L ++D+   K +   KFN  E+N++ W+  G++  +   
Sbjct: 273 NIDVRWSPDGQFIVACTRDDHLALIDLPTIKTLKIYKFNGEELNQVGWDNNGDLILMANS 332

Query: 188 NGTVEVLAY-----PSLRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQML 247
            G +E   +       ++ ++TL  HTA  YC+  DP G Y A GSADS+VSLWDI  M+
Sbjct: 333 MGNIEAYKFLPKSTTHVKHLKTLYGHTASIYCMEFDPTGKYLAAGSADSIVSLWDIEDMM 392

Query: 248 CVRTFTKLEWPVRTISFNHTGEYIASASEDLFIDISSVQSGRTVHQIPCRAAMNSVEWNP 307
           CV+TF K  +P R++SF+  G++IA++S +  I+I  ++S + +H I C + ++S+ W+P
Sbjct: 393 CVKTFIKSTFPCRSVSFSFDGQFIAASSFESTIEIFHIESSQPIHTIEC-SGVSSLMWHP 452

Query: 308 KHNLLAYAGDDKNKYQADEGKLSMRIFRIFGFES 319
              LLAYA  + N+   D         R+FG+ S
Sbjct: 453 TLPLLAYA-PEINENNKDPS------IRVFGYHS 478

BLAST of Cp4.1LG08g08500 vs. TrEMBL
Match: A0A0A0L651_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G061010 PE=4 SV=1)

HSP 1 Score: 644.4 bits (1661), Expect = 7.1e-182
Identity = 308/318 (96.86%), Postives = 311/318 (97.80%), Query Frame = 1

Query: 1   MEESTPAFKNLYSREYQGHKKKVHSVAWNCTGMKLASGSVDQTARVWHIEPHGHGKVKDV 60
           MEES  AFKNL+SREYQGHKKKVHSVAWNCTGMKLASGSVDQTARVWHIEPHGHGKVKDV
Sbjct: 1   MEESAQAFKNLHSREYQGHKKKVHSVAWNCTGMKLASGSVDQTARVWHIEPHGHGKVKDV 60

Query: 61  ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDG 120
           ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDG
Sbjct: 61  ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDG 120

Query: 121 THIAVGNRDDELTILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS 180
           THIAVGNRDDELTILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS
Sbjct: 121 THIAVGNRDDELTILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS 180

Query: 181 LRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS 240
           LRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS
Sbjct: 181 LRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS 240

Query: 241 FNHTGEYIASASEDLFIDISSVQSGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQ 300
           FNHTGEYIASASEDLFIDIS+VQ+GRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQ
Sbjct: 241 FNHTGEYIASASEDLFIDISNVQTGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQ 300

Query: 301 ADEGKLSMRIFRIFGFES 319
           ADEG     IFRIFGFES
Sbjct: 301 ADEG-----IFRIFGFES 313

BLAST of Cp4.1LG08g08500 vs. TrEMBL
Match: A0A059BRQ3_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_F01999 PE=4 SV=1)

HSP 1 Score: 621.3 bits (1601), Expect = 6.5e-175
Identity = 293/319 (91.85%), Postives = 309/319 (96.87%), Query Frame = 1

Query: 1   MEESTPAFKNLYSREYQGHKKKVHSVAWNCTGMKLASGSVDQTARVWHIEPHGHGKVKDV 60
           MEE+ P FKNL SREYQGHKKKVHSVAWNCTG KLASGSVDQTARVWHIEPHGHGKVKD+
Sbjct: 63  MEEAIP-FKNLPSREYQGHKKKVHSVAWNCTGTKLASGSVDQTARVWHIEPHGHGKVKDI 122

Query: 61  ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDG 120
           ELKGHTDSVDQLCWDPKH+DLIATASGDKTVRLWDAR+GKCSQQAELSGENINITYKPDG
Sbjct: 123 ELKGHTDSVDQLCWDPKHADLIATASGDKTVRLWDARSGKCSQQAELSGENINITYKPDG 182

Query: 121 THIAVGNRDDELTILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS 180
           TH+AVGNRDDELTILDVRKFKP+HKRKFNYEVNEIAWNM+GEMFFLTTGNGTVEVLAYPS
Sbjct: 183 THVAVGNRDDELTILDVRKFKPIHKRKFNYEVNEIAWNMSGEMFFLTTGNGTVEVLAYPS 242

Query: 181 LRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS 240
           LRP++TLMAHTAGCYCIAIDPVG YFAVGSADSLVSLWDIS+MLCVRTFTKLEWPVRTIS
Sbjct: 243 LRPVDTLMAHTAGCYCIAIDPVGRYFAVGSADSLVSLWDISEMLCVRTFTKLEWPVRTIS 302

Query: 241 FNHTGEYIASASEDLFIDISSVQSGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQ 300
           FNHTG+Y+ASASEDLFIDIS+VQ+GRTVHQIPCRAAMNSVEWNPK+NLLAYAGDDKNKYQ
Sbjct: 303 FNHTGDYVASASEDLFIDISNVQTGRTVHQIPCRAAMNSVEWNPKYNLLAYAGDDKNKYQ 362

Query: 301 ADEGKLSMRIFRIFGFESA 320
           ADEG     +FRIFGFESA
Sbjct: 363 ADEG-----VFRIFGFESA 375

BLAST of Cp4.1LG08g08500 vs. TrEMBL
Match: A5ADM7_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_19s0015g01220 PE=4 SV=1)

HSP 1 Score: 613.2 bits (1580), Expect = 1.8e-172
Identity = 289/319 (90.60%), Postives = 307/319 (96.24%), Query Frame = 1

Query: 1   MEESTPAFKNLYSREYQGHKKKVHSVAWNCTGMKLASGSVDQTARVWHIEPHGHGKVKDV 60
           MEE+ P FKNL+SREYQGHKKKVHSVAWNCTG KLASGSVDQTAR+W IE HGHGKVKD+
Sbjct: 1   MEETIP-FKNLHSREYQGHKKKVHSVAWNCTGTKLASGSVDQTARIWLIEQHGHGKVKDI 60

Query: 61  ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDG 120
           ELKGHTDSVDQLCWDPKH+DLIATASGDKTVRLWDAR+GKC+QQAELSGENINITYKPDG
Sbjct: 61  ELKGHTDSVDQLCWDPKHADLIATASGDKTVRLWDARSGKCTQQAELSGENINITYKPDG 120

Query: 121 THIAVGNRDDELTILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS 180
           THIAVGNRDDELTILDVRKFKP+H+RKF+YEVNEIAWNMTGEMFFLTTGNGTVEVLAYP+
Sbjct: 121 THIAVGNRDDELTILDVRKFKPIHRRKFSYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPA 180

Query: 181 LRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS 240
           LRP++TLMAHTAGCYCIAIDP+G YFAVGSADSLVSLWDIS+MLCVRTFTKLEWPVRTIS
Sbjct: 181 LRPLDTLMAHTAGCYCIAIDPIGRYFAVGSADSLVSLWDISEMLCVRTFTKLEWPVRTIS 240

Query: 241 FNHTGEYIASASEDLFIDISSVQSGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQ 300
           FNHTGEYIASASEDLFIDIS+V +GRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQ
Sbjct: 241 FNHTGEYIASASEDLFIDISNVHTGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQ 300

Query: 301 ADEGKLSMRIFRIFGFESA 320
           ADEG     +FRIFGFESA
Sbjct: 301 ADEG-----VFRIFGFESA 313

BLAST of Cp4.1LG08g08500 vs. TrEMBL
Match: A0A151UA77_CAJCA (THO complex subunit 3 OS=Cajanus cajan GN=KK1_020463 PE=4 SV=1)

HSP 1 Score: 612.1 bits (1577), Expect = 3.9e-172
Identity = 286/319 (89.66%), Postives = 307/319 (96.24%), Query Frame = 1

Query: 1   MEESTPAFKNLYSREYQGHKKKVHSVAWNCTGMKLASGSVDQTARVWHIEPHGHGKVKDV 60
           MEE  P FKNL+SREY GHKKKVHSVAWNCTG KLASGSVDQTAR+WHIEPHGHGKVKD+
Sbjct: 1   MEEQIP-FKNLHSREYSGHKKKVHSVAWNCTGTKLASGSVDQTARIWHIEPHGHGKVKDI 60

Query: 61  ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDG 120
           ELKGHTDSVDQLCWDPKH+DLIATASGDKTVRLWDAR+GKCSQQAELSGENINITYKPDG
Sbjct: 61  ELKGHTDSVDQLCWDPKHADLIATASGDKTVRLWDARSGKCSQQAELSGENINITYKPDG 120

Query: 121 THIAVGNRDDELTILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS 180
           TH+AVGNRDDELTILDVRKFKP+H+RKFNYEVNEIAWNMTGEMFFLTTGNGTVEVL+YPS
Sbjct: 121 THVAVGNRDDELTILDVRKFKPIHRRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLSYPS 180

Query: 181 LRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS 240
           LRP++TLMAHTAGCYCIAIDP+G YFAVGSADSLVSLWDIS+MLCVRTFTKLEWPVRTI 
Sbjct: 181 LRPLDTLMAHTAGCYCIAIDPMGRYFAVGSADSLVSLWDISEMLCVRTFTKLEWPVRTIG 240

Query: 241 FNHTGEYIASASEDLFIDISSVQSGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQ 300
           FN+TG++IASASEDLFIDIS+VQ+GRTVHQIPCRAAMNSVEWNPK+NLLAYAGDDKNKYQ
Sbjct: 241 FNYTGDFIASASEDLFIDISNVQAGRTVHQIPCRAAMNSVEWNPKYNLLAYAGDDKNKYQ 300

Query: 301 ADEGKLSMRIFRIFGFESA 320
           ADEG     +FRIFGFE+A
Sbjct: 301 ADEG-----VFRIFGFENA 313

BLAST of Cp4.1LG08g08500 vs. TrEMBL
Match: W9QYM4_9ROSA (THO complex subunit 3 OS=Morus notabilis GN=L484_023771 PE=4 SV=1)

HSP 1 Score: 610.1 bits (1572), Expect = 1.5e-171
Identity = 288/319 (90.28%), Postives = 306/319 (95.92%), Query Frame = 1

Query: 1   MEESTPAFKNLYSREYQGHKKKVHSVAWNCTGMKLASGSVDQTARVWHIEPHGHGKVKDV 60
           MEE+ P FKNL+SREYQGHKKKVHSVAWNC G KLASGSVDQTARVWHIEPHGH KVKD+
Sbjct: 1   MEETIP-FKNLHSREYQGHKKKVHSVAWNCNGTKLASGSVDQTARVWHIEPHGHVKVKDI 60

Query: 61  ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDG 120
           ELKGHTDSVDQLCWDPKH+DLIATASGDKTVRLWDAR+GKCSQQA+LSGENINITYKPDG
Sbjct: 61  ELKGHTDSVDQLCWDPKHADLIATASGDKTVRLWDARSGKCSQQADLSGENINITYKPDG 120

Query: 121 THIAVGNRDDELTILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS 180
           T+IAVGNRDDELTILDVRKFK +HKRKFNYEVNEIAWNMTG+MFFLTTGNGTVEVLAYPS
Sbjct: 121 TYIAVGNRDDELTILDVRKFKAIHKRKFNYEVNEIAWNMTGDMFFLTTGNGTVEVLAYPS 180

Query: 181 LRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS 240
           LRP++TLMAHTAGCYCIAIDP+G YFAVGSADSLVSLWDIS+MLCVRTFTKLEWPVRTIS
Sbjct: 181 LRPLDTLMAHTAGCYCIAIDPIGRYFAVGSADSLVSLWDISEMLCVRTFTKLEWPVRTIS 240

Query: 241 FNHTGEYIASASEDLFIDISSVQSGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQ 300
           FNHTGEYIASASEDLFIDIS+VQ+GRTVHQIPCRAAMNSVEWNPK+NLL YAGDDKNKYQ
Sbjct: 241 FNHTGEYIASASEDLFIDISNVQTGRTVHQIPCRAAMNSVEWNPKYNLLVYAGDDKNKYQ 300

Query: 301 ADEGKLSMRIFRIFGFESA 320
           ADEG     +FRIFGFES+
Sbjct: 301 ADEG-----VFRIFGFESS 313

BLAST of Cp4.1LG08g08500 vs. TAIR10
Match: AT5G56130.1 (AT5G56130.1 Transducin/WD40 repeat-like superfamily protein)

HSP 1 Score: 575.9 bits (1483), Expect = 1.6e-164
Identity = 270/320 (84.38%), Postives = 297/320 (92.81%), Query Frame = 1

Query: 1   MEESTPAFKNLYSREYQGHKKKVHSVAWNCTGMKLASGSVDQTARVWHIEPHGHGKVKDV 60
           MEE+T  FK+L+SREYQGHKKKVHSVAWN  G KLASGSVDQTAR+W+IEPHGH K KD+
Sbjct: 1   MEETTIPFKSLHSREYQGHKKKVHSVAWNSNGTKLASGSVDQTARIWNIEPHGHSKAKDL 60

Query: 61  ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDG 120
           ELKGHTDSVDQLCWDPKHSDL+ATASGDK+VRLWDAR+GKC+QQ ELSGENINITYKPDG
Sbjct: 61  ELKGHTDSVDQLCWDPKHSDLVATASGDKSVRLWDARSGKCTQQVELSGENINITYKPDG 120

Query: 121 THIAVGNRDDELTILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS 180
           TH+AVGNRDDELTILDVRKFKP+H+RKFNYEVNEIAWNM G+ FFLTTG GTVEVL+YPS
Sbjct: 121 THVAVGNRDDELTILDVRKFKPLHRRKFNYEVNEIAWNMPGDFFFLTTGLGTVEVLSYPS 180

Query: 181 LRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS 240
           L+P++TL AHTAGCYCIAIDP G YFAVGSADSLVSLWDIS MLC+RTFTKLEWPVRTIS
Sbjct: 181 LKPLDTLTAHTAGCYCIAIDPKGRYFAVGSADSLVSLWDISDMLCLRTFTKLEWPVRTIS 240

Query: 241 FNHTGEYIASASEDLFIDISSVQSGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKN-KY 300
           FN++GEYIASASEDLFIDI++VQ+GRTVHQIPCRAAMNSVEWNPK+NLLAYAGDDKN KY
Sbjct: 241 FNYSGEYIASASEDLFIDIANVQTGRTVHQIPCRAAMNSVEWNPKYNLLAYAGDDKNPKY 300

Query: 301 QADEGKLSMRIFRIFGFESA 320
             DEG     +FRIFGFES+
Sbjct: 301 NTDEG-----VFRIFGFESS 315

BLAST of Cp4.1LG08g08500 vs. TAIR10
Match: AT5G67320.1 (AT5G67320.1 WD-40 repeat family protein)

HSP 1 Score: 108.2 bits (269), Expect = 9.3e-24
Identity = 81/283 (28.62%), Postives = 130/283 (45.94%), Query Frame = 1

Query: 21  KKVHSVAWNCTGMKLASGSVDQTARVWHIEPHGHGKVKDVELKGHTDSVDQLCWDPKHSD 80
           K V ++ WN  G  LA+GS D  AR+W +    +G++    L  H   +  L W+ K  D
Sbjct: 325 KDVTTLDWNGEGTLLATGSCDGQARIWTL----NGELIST-LSKHKGPIFSLKWNKK-GD 384

Query: 81  LIATASGDKTVRLWDARNGKCSQQAEL-SGENINITYKPDGTHIAVGNRDDELTILDVRK 140
            + T S D+T  +WD +  +  QQ E  SG  +++ ++ +    A  + D  + +  + +
Sbjct: 385 YLLTGSVDRTAVVWDVKAEEWKQQFEFHSGPTLDVDWR-NNVSFATSSTDSMIYLCKIGE 444

Query: 141 FKPVHKRKFNY-EVNEIAWNMTGEMFFLTTGNGTVEVLAYPSLRPIETLMAHTAGCYCIA 200
            +P      +  EVN + W+ TG +    + + T ++        +  L  HT   Y I 
Sbjct: 445 TRPAKTFTGHQGEVNCVKWDPTGSLLASCSDDSTAKIWNIKQSTFVHDLREHTKEIYTIR 504

Query: 201 IDPVGG---------YFAVGSADSLVSLWD--ISQMLCVRTFTKLEWPVRTISFNHTGEY 260
             P G            A  S DS V LWD  + +MLC  +F     PV +++F+  GEY
Sbjct: 505 WSPTGPGTNNPNKQLTLASASFDSTVKLWDAELGKMLC--SFNGHREPVYSLAFSPNGEY 564

Query: 261 IASASEDLFIDISSVQSGRTVHQIPCRAAMNSVEWNPKHNLLA 291
           IAS S D  I I S++ G+ V        +  V WN + N +A
Sbjct: 565 IASGSLDKSIHIWSIKEGKIVKTYTGNGGIFEVCWNKEGNKIA 598

BLAST of Cp4.1LG08g08500 vs. TAIR10
Match: AT2G43770.1 (AT2G43770.1 Transducin/WD40 repeat-like superfamily protein)

HSP 1 Score: 97.8 bits (242), Expect = 1.3e-20
Identity = 74/294 (25.17%), Postives = 130/294 (44.22%), Query Frame = 1

Query: 18  GHKKKVHSVAWNCTGMKLASGSVDQTARVWHIEPHGHGKVKD-VELKGHTDSVDQLCWDP 77
           GH   V+++ +N  G  +ASGS D+   +W +    HG  K+ + LKGH +++  L W  
Sbjct: 51  GHPSAVYTMKFNPAGTLIASGSHDREIFLWRV----HGDCKNFMVLKGHKNAILDLHWTS 110

Query: 78  KHSDLIATASGDKTVRLWDARNGK-CSQQAELSGENINITYKPDGTHIAVGNRDDELTIL 137
             S ++ +AS DKTVR WD   GK   + AE S    +      G  + +   DD    L
Sbjct: 111 DGSQIV-SASPDKTVRAWDVETGKQIKKMAEHSSFVNSCCPTRRGPPLIISGSDDGTAKL 170

Query: 138 -DVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPSLRPIETLMAHTAGC 197
            D+R+   +      Y++  ++++   +  F    +  V+V          TL  H    
Sbjct: 171 WDMRQRGAIQTFPDKYQITAVSFSDAADKIFTGGVDNDVKVWDLRKGEATMTLEGHQDTI 230

Query: 198 YCIAIDPVGGYFAVGSADSLVSLWDI----SQMLCVRTFT----KLEWPVRTISFNHTGE 257
             +++ P G Y      D+ + +WD+     Q  CV+ F       E  +   S++  G 
Sbjct: 231 TGMSLSPDGSYLLTNGMDNKLCVWDMRPYAPQNRCVKIFEGHQHNFEKNLLKCSWSPDGT 290

Query: 258 YIASASEDLFIDISSVQSGRTVHQIPCR-AAMNSVEWNPKHNLLAYAGDDKNKY 300
            + + S D  + I    S RT++++P    ++N   ++P   ++     DKN Y
Sbjct: 291 KVTAGSSDRMVHIWDTTSRRTIYKLPGHTGSVNECVFHPTEPIIGSCSSDKNIY 339

BLAST of Cp4.1LG08g08500 vs. TAIR10
Match: AT3G49660.1 (AT3G49660.1 Transducin/WD40 repeat-like superfamily protein)

HSP 1 Score: 84.0 bits (206), Expect = 1.9e-16
Identity = 76/314 (24.20%), Postives = 136/314 (43.31%), Query Frame = 1

Query: 1   MEESTPAFKN----LYSREYQGHKKKVHSVAWNCTGMKLASGSVDQTARVWHIEPHGHGK 60
           M E  PA  +    ++S+    H + V SV ++  G  LAS S D+T R + I       
Sbjct: 1   MAEEIPATASFTPYVHSQTLTSHNRAVSSVKFSSDGRLLASASADKTIRTYTINTINDPI 60

Query: 61  VKDV-ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGEN---I 120
            + V E  GH + +  + +    +  I +AS DKT++LWD   G  S    L G      
Sbjct: 61  AEPVQEFTGHENGISDVAFS-SDARFIVSASDDKTLKLWDVETG--SLIKTLIGHTNYAF 120

Query: 121 NITYKPDGTHIAVGNRDDELTILDVR-----KFKPVHKRKFNYEVNEIAWNMTGEMFFLT 180
            + + P    I  G+ D+ + I DV      K  P H    +  V  + +N  G +   +
Sbjct: 121 CVNFNPQSNMIVSGSFDETVRIWDVTTGKCLKVLPAH----SDPVTAVDFNRDGSLIVSS 180

Query: 181 TGNGTVEVLAYPSLRPIETLM-AHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCV 240
           + +G   +    +   ++TL+         +   P G +  VG+ D+ + LW+IS    +
Sbjct: 181 SYDGLCRIWDSGTGHCVKTLIDDENPPVSFVRFSPNGKFILVGTLDNTLRLWNISSAKFL 240

Query: 241 RTFT---KLEWPVRTISFNHTGEYIASASEDLFIDISSVQSGRTVHQIPCRA-AMNSVEW 297
           +T+T     ++ + +      G+ I S SED  + +  + S + + ++      + +V  
Sbjct: 241 KTYTGHVNAQYCISSAFSVTNGKRIVSGSEDNCVHMWELNSKKLLQKLEGHTETVMNVAC 300

BLAST of Cp4.1LG08g08500 vs. TAIR10
Match: AT4G02730.1 (AT4G02730.1 Transducin/WD40 repeat-like superfamily protein)

HSP 1 Score: 81.6 bits (200), Expect = 9.4e-16
Identity = 59/213 (27.70%), Postives = 104/213 (48.83%), Query Frame = 1

Query: 16  YQGHKKKVHSVAWNCTGMKLASGSVDQTARVWHIE-PHGHGKVKDVELKGHTDSVDQLCW 75
           Y+GH   +  +AW+       S S D T R+W    P+   KV    L+GHT+ V  + +
Sbjct: 81  YEGHSSGISDLAWSSDSHYTCSASDDCTLRIWDARSPYECLKV----LRGHTNFVFCVNF 140

Query: 76  DPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENI-NITYKPDGTHIAVGNRDDELT 135
           +P  S+LI + S D+T+R+W+ + GKC +  +     I ++ +  DG+ I   + D    
Sbjct: 141 NPP-SNLIVSGSFDETIRIWEVKTGKCVRMIKAHSMPISSVHFNRDGSLIVSASHDGSCK 200

Query: 136 ILDVRK---FKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPSLRPIETLMAH 195
           I D ++    K +   K +  V+   ++  G+   + T + T+++  Y + + ++    H
Sbjct: 201 IWDAKEGTCLKTLIDDK-SPAVSFAKFSPNGKFILVATLDSTLKLSNYATGKFLKVYTGH 260

Query: 196 TAGCYCI--AIDPVGG-YFAVGSADSLVSLWDI 221
           T   +CI  A     G Y   GS D+ V LWD+
Sbjct: 261 TNKVFCITSAFSVTNGKYIVSGSEDNCVYLWDL 287

BLAST of Cp4.1LG08g08500 vs. NCBI nr
Match: gi|449463705|ref|XP_004149572.1| (PREDICTED: THO complex subunit 3 [Cucumis sativus])

HSP 1 Score: 644.4 bits (1661), Expect = 1.0e-181
Identity = 308/318 (96.86%), Postives = 311/318 (97.80%), Query Frame = 1

Query: 1   MEESTPAFKNLYSREYQGHKKKVHSVAWNCTGMKLASGSVDQTARVWHIEPHGHGKVKDV 60
           MEES  AFKNL+SREYQGHKKKVHSVAWNCTGMKLASGSVDQTARVWHIEPHGHGKVKDV
Sbjct: 1   MEESAQAFKNLHSREYQGHKKKVHSVAWNCTGMKLASGSVDQTARVWHIEPHGHGKVKDV 60

Query: 61  ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDG 120
           ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDG
Sbjct: 61  ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDG 120

Query: 121 THIAVGNRDDELTILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS 180
           THIAVGNRDDELTILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS
Sbjct: 121 THIAVGNRDDELTILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS 180

Query: 181 LRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS 240
           LRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS
Sbjct: 181 LRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS 240

Query: 241 FNHTGEYIASASEDLFIDISSVQSGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQ 300
           FNHTGEYIASASEDLFIDIS+VQ+GRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQ
Sbjct: 241 FNHTGEYIASASEDLFIDISNVQTGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQ 300

Query: 301 ADEGKLSMRIFRIFGFES 319
           ADEG     IFRIFGFES
Sbjct: 301 ADEG-----IFRIFGFES 313

BLAST of Cp4.1LG08g08500 vs. NCBI nr
Match: gi|629102866|gb|KCW68335.1| (hypothetical protein EUGRSUZ_F01999 [Eucalyptus grandis])

HSP 1 Score: 621.3 bits (1601), Expect = 9.3e-175
Identity = 293/319 (91.85%), Postives = 309/319 (96.87%), Query Frame = 1

Query: 1   MEESTPAFKNLYSREYQGHKKKVHSVAWNCTGMKLASGSVDQTARVWHIEPHGHGKVKDV 60
           MEE+ P FKNL SREYQGHKKKVHSVAWNCTG KLASGSVDQTARVWHIEPHGHGKVKD+
Sbjct: 63  MEEAIP-FKNLPSREYQGHKKKVHSVAWNCTGTKLASGSVDQTARVWHIEPHGHGKVKDI 122

Query: 61  ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDG 120
           ELKGHTDSVDQLCWDPKH+DLIATASGDKTVRLWDAR+GKCSQQAELSGENINITYKPDG
Sbjct: 123 ELKGHTDSVDQLCWDPKHADLIATASGDKTVRLWDARSGKCSQQAELSGENINITYKPDG 182

Query: 121 THIAVGNRDDELTILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS 180
           TH+AVGNRDDELTILDVRKFKP+HKRKFNYEVNEIAWNM+GEMFFLTTGNGTVEVLAYPS
Sbjct: 183 THVAVGNRDDELTILDVRKFKPIHKRKFNYEVNEIAWNMSGEMFFLTTGNGTVEVLAYPS 242

Query: 181 LRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS 240
           LRP++TLMAHTAGCYCIAIDPVG YFAVGSADSLVSLWDIS+MLCVRTFTKLEWPVRTIS
Sbjct: 243 LRPVDTLMAHTAGCYCIAIDPVGRYFAVGSADSLVSLWDISEMLCVRTFTKLEWPVRTIS 302

Query: 241 FNHTGEYIASASEDLFIDISSVQSGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQ 300
           FNHTG+Y+ASASEDLFIDIS+VQ+GRTVHQIPCRAAMNSVEWNPK+NLLAYAGDDKNKYQ
Sbjct: 303 FNHTGDYVASASEDLFIDISNVQTGRTVHQIPCRAAMNSVEWNPKYNLLAYAGDDKNKYQ 362

Query: 301 ADEGKLSMRIFRIFGFESA 320
           ADEG     +FRIFGFESA
Sbjct: 363 ADEG-----VFRIFGFESA 375

BLAST of Cp4.1LG08g08500 vs. NCBI nr
Match: gi|702369740|ref|XP_010061402.1| (PREDICTED: THO complex subunit 3 [Eucalyptus grandis])

HSP 1 Score: 621.3 bits (1601), Expect = 9.3e-175
Identity = 293/319 (91.85%), Postives = 309/319 (96.87%), Query Frame = 1

Query: 1   MEESTPAFKNLYSREYQGHKKKVHSVAWNCTGMKLASGSVDQTARVWHIEPHGHGKVKDV 60
           MEE+ P FKNL SREYQGHKKKVHSVAWNCTG KLASGSVDQTARVWHIEPHGHGKVKD+
Sbjct: 1   MEEAIP-FKNLPSREYQGHKKKVHSVAWNCTGTKLASGSVDQTARVWHIEPHGHGKVKDI 60

Query: 61  ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDG 120
           ELKGHTDSVDQLCWDPKH+DLIATASGDKTVRLWDAR+GKCSQQAELSGENINITYKPDG
Sbjct: 61  ELKGHTDSVDQLCWDPKHADLIATASGDKTVRLWDARSGKCSQQAELSGENINITYKPDG 120

Query: 121 THIAVGNRDDELTILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS 180
           TH+AVGNRDDELTILDVRKFKP+HKRKFNYEVNEIAWNM+GEMFFLTTGNGTVEVLAYPS
Sbjct: 121 THVAVGNRDDELTILDVRKFKPIHKRKFNYEVNEIAWNMSGEMFFLTTGNGTVEVLAYPS 180

Query: 181 LRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS 240
           LRP++TLMAHTAGCYCIAIDPVG YFAVGSADSLVSLWDIS+MLCVRTFTKLEWPVRTIS
Sbjct: 181 LRPVDTLMAHTAGCYCIAIDPVGRYFAVGSADSLVSLWDISEMLCVRTFTKLEWPVRTIS 240

Query: 241 FNHTGEYIASASEDLFIDISSVQSGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQ 300
           FNHTG+Y+ASASEDLFIDIS+VQ+GRTVHQIPCRAAMNSVEWNPK+NLLAYAGDDKNKYQ
Sbjct: 241 FNHTGDYVASASEDLFIDISNVQTGRTVHQIPCRAAMNSVEWNPKYNLLAYAGDDKNKYQ 300

Query: 301 ADEGKLSMRIFRIFGFESA 320
           ADEG     +FRIFGFESA
Sbjct: 301 ADEG-----VFRIFGFESA 313

BLAST of Cp4.1LG08g08500 vs. NCBI nr
Match: gi|225462041|ref|XP_002274754.1| (PREDICTED: THO complex subunit 3 [Vitis vinifera])

HSP 1 Score: 613.2 bits (1580), Expect = 2.5e-172
Identity = 289/319 (90.60%), Postives = 307/319 (96.24%), Query Frame = 1

Query: 1   MEESTPAFKNLYSREYQGHKKKVHSVAWNCTGMKLASGSVDQTARVWHIEPHGHGKVKDV 60
           MEE+ P FKNL+SREYQGHKKKVHSVAWNCTG KLASGSVDQTAR+W IE HGHGKVKD+
Sbjct: 1   MEETIP-FKNLHSREYQGHKKKVHSVAWNCTGTKLASGSVDQTARIWLIEQHGHGKVKDI 60

Query: 61  ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDG 120
           ELKGHTDSVDQLCWDPKH+DLIATASGDKTVRLWDAR+GKC+QQAELSGENINITYKPDG
Sbjct: 61  ELKGHTDSVDQLCWDPKHADLIATASGDKTVRLWDARSGKCTQQAELSGENINITYKPDG 120

Query: 121 THIAVGNRDDELTILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS 180
           THIAVGNRDDELTILDVRKFKP+H+RKF+YEVNEIAWNMTGEMFFLTTGNGTVEVLAYP+
Sbjct: 121 THIAVGNRDDELTILDVRKFKPIHRRKFSYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPA 180

Query: 181 LRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS 240
           LRP++TLMAHTAGCYCIAIDP+G YFAVGSADSLVSLWDIS+MLCVRTFTKLEWPVRTIS
Sbjct: 181 LRPLDTLMAHTAGCYCIAIDPIGRYFAVGSADSLVSLWDISEMLCVRTFTKLEWPVRTIS 240

Query: 241 FNHTGEYIASASEDLFIDISSVQSGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQ 300
           FNHTGEYIASASEDLFIDIS+V +GRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQ
Sbjct: 241 FNHTGEYIASASEDLFIDISNVHTGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQ 300

Query: 301 ADEGKLSMRIFRIFGFESA 320
           ADEG     +FRIFGFESA
Sbjct: 301 ADEG-----VFRIFGFESA 313

BLAST of Cp4.1LG08g08500 vs. NCBI nr
Match: gi|1012365048|gb|KYP76230.1| (THO complex subunit 3 [Cajanus cajan])

HSP 1 Score: 612.1 bits (1577), Expect = 5.6e-172
Identity = 286/319 (89.66%), Postives = 307/319 (96.24%), Query Frame = 1

Query: 1   MEESTPAFKNLYSREYQGHKKKVHSVAWNCTGMKLASGSVDQTARVWHIEPHGHGKVKDV 60
           MEE  P FKNL+SREY GHKKKVHSVAWNCTG KLASGSVDQTAR+WHIEPHGHGKVKD+
Sbjct: 1   MEEQIP-FKNLHSREYSGHKKKVHSVAWNCTGTKLASGSVDQTARIWHIEPHGHGKVKDI 60

Query: 61  ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDG 120
           ELKGHTDSVDQLCWDPKH+DLIATASGDKTVRLWDAR+GKCSQQAELSGENINITYKPDG
Sbjct: 61  ELKGHTDSVDQLCWDPKHADLIATASGDKTVRLWDARSGKCSQQAELSGENINITYKPDG 120

Query: 121 THIAVGNRDDELTILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS 180
           TH+AVGNRDDELTILDVRKFKP+H+RKFNYEVNEIAWNMTGEMFFLTTGNGTVEVL+YPS
Sbjct: 121 THVAVGNRDDELTILDVRKFKPIHRRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLSYPS 180

Query: 181 LRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS 240
           LRP++TLMAHTAGCYCIAIDP+G YFAVGSADSLVSLWDIS+MLCVRTFTKLEWPVRTI 
Sbjct: 181 LRPLDTLMAHTAGCYCIAIDPMGRYFAVGSADSLVSLWDISEMLCVRTFTKLEWPVRTIG 240

Query: 241 FNHTGEYIASASEDLFIDISSVQSGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQ 300
           FN+TG++IASASEDLFIDIS+VQ+GRTVHQIPCRAAMNSVEWNPK+NLLAYAGDDKNKYQ
Sbjct: 241 FNYTGDFIASASEDLFIDISNVQAGRTVHQIPCRAAMNSVEWNPKYNLLAYAGDDKNKYQ 300

Query: 301 ADEGKLSMRIFRIFGFESA 320
           ADEG     +FRIFGFE+A
Sbjct: 301 ADEG-----VFRIFGFENA 313

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
THOC3_ARATH2.8e-16384.38THO complex subunit 3 OS=Arabidopsis thaliana GN=THO3 PE=1 SV=1[more]
THOC3_MOUSE6.4e-9150.33THO complex subunit 3 OS=Mus musculus GN=Thoc3 PE=2 SV=1[more]
THOC3_BOVIN6.4e-9150.33THO complex subunit 3 OS=Bos taurus GN=THOC3 PE=2 SV=1[more]
THOC3_HUMAN8.3e-9150.33THO complex subunit 3 OS=Homo sapiens GN=THOC3 PE=1 SV=1[more]
AAC3_DICDI1.6e-6235.93WD repeat-containing protein AAC3 OS=Dictyostelium discoideum GN=AAC3 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A0A0L651_CUCSA7.1e-18296.86Uncharacterized protein OS=Cucumis sativus GN=Csa_3G061010 PE=4 SV=1[more]
A0A059BRQ3_EUCGR6.5e-17591.85Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_F01999 PE=4 SV=1[more]
A5ADM7_VITVI1.8e-17290.60Putative uncharacterized protein OS=Vitis vinifera GN=VIT_19s0015g01220 PE=4 SV=... [more]
A0A151UA77_CAJCA3.9e-17289.66THO complex subunit 3 OS=Cajanus cajan GN=KK1_020463 PE=4 SV=1[more]
W9QYM4_9ROSA1.5e-17190.28THO complex subunit 3 OS=Morus notabilis GN=L484_023771 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G56130.11.6e-16484.38 Transducin/WD40 repeat-like superfamily protein[more]
AT5G67320.19.3e-2428.62 WD-40 repeat family protein[more]
AT2G43770.11.3e-2025.17 Transducin/WD40 repeat-like superfamily protein[more]
AT3G49660.11.9e-1624.20 Transducin/WD40 repeat-like superfamily protein[more]
AT4G02730.19.4e-1627.70 Transducin/WD40 repeat-like superfamily protein[more]
Match NameE-valueIdentityDescription
gi|449463705|ref|XP_004149572.1|1.0e-18196.86PREDICTED: THO complex subunit 3 [Cucumis sativus][more]
gi|629102866|gb|KCW68335.1|9.3e-17591.85hypothetical protein EUGRSUZ_F01999 [Eucalyptus grandis][more]
gi|702369740|ref|XP_010061402.1|9.3e-17591.85PREDICTED: THO complex subunit 3 [Eucalyptus grandis][more]
gi|225462041|ref|XP_002274754.1|2.5e-17290.60PREDICTED: THO complex subunit 3 [Vitis vinifera][more]
gi|1012365048|gb|KYP76230.1|5.6e-17289.66THO complex subunit 3 [Cajanus cajan][more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0005515protein binding
Vocabulary: INTERPRO
TermDefinition
IPR024977Apc4_WD40_dom
IPR020472G-protein_beta_WD-40_rep
IPR019775WD40_repeat_CS
IPR017986WD40_repeat_dom
IPR015943WD40/YVTN_repeat-like_dom_sf
IPR001680WD40_repeat
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010267 production of ta-siRNAs involved in RNA interference
biological_process GO:0008150 biological_process
biological_process GO:0006406 mRNA export from nucleus
cellular_component GO:0080008 Cul4-RING E3 ubiquitin ligase complex
cellular_component GO:0000347 THO complex
cellular_component GO:0005575 cellular_component
cellular_component GO:0000445 THO complex part of transcription export complex
molecular_function GO:0005515 protein binding
molecular_function GO:0003674 molecular_function

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG08g08500.1Cp4.1LG08g08500.1mRNA


Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001680WD40 repeatPFAMPF00400WD40coord: 184..219
score: 0.028coord: 61..95
score: 2.7E-4coord: 14..47
score: 1.
IPR001680WD40 repeatSMARTSM00320WD40_4coord: 9..48
score: 3.1E-7coord: 264..312
score: 54.0coord: 180..219
score: 6.7E-8coord: 55..95
score: 2.6E-8coord: 139..177
score: 150.0coord: 222..261
score:
IPR001680WD40 repeatPROFILEPS50082WD_REPEATS_2coord: 62..104
score: 15.555coord: 16..50
score: 13.215coord: 187..228
score: 12
IPR015943WD40/YVTN repeat-like-containing domainGENE3DG3DSA:2.130.10.10coord: 164..297
score: 6.4E-29coord: 14..163
score: 3.8
IPR017986WD40-repeat-containing domainPROFILEPS50294WD_REPEATS_REGIONcoord: 16..270
score: 28
IPR017986WD40-repeat-containing domainunknownSSF50978WD40 repeat-likecoord: 11..296
score: 9.77
IPR019775WD40 repeat, conserved sitePROSITEPS00678WD_REPEATS_1coord: 82..96
scor
IPR020472G-protein beta WD-40 repeatPRINTSPR00320GPROTEINBRPTcoord: 82..96
score: 2.5E-7coord: 35..49
score: 2.5E-7coord: 206..220
score: 2.
IPR024977Anaphase-promoting complex subunit 4, WD40 domainPFAMPF12894ANAPC4_WD40coord: 113..159
score: 1.9E-4coord: 232..283
score: 1.
NoneNo IPR availablePANTHERPTHR22839THO COMPLEX SUBUNIT 3 THO3coord: 1..318
score: 5.7E