Cp4.1LG05g02620 (gene) Cucurbita pepo (Zucchini)

Overview
NameCp4.1LG05g02620
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
DescriptionPlant invertase/pectin methylesterase inhibitor superfamily protein
LocationCp4.1LG05 : 1497887 .. 1498471 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAAATCTCAAGATCTCACTCCCACTTCTCGTCCTTGTAGTCCTCCACAATGCCGCACCCACACACTCAGCCACCGCCACCAGCTTCATTGAGTCCTCCTGCAGAGTCACTCGCTACCCGGCGCTCTGCGTCCACTCCCTCTCAGCCTACGCCACTTCCATCCGCCAGAGCGGCCGTCAGCTCACCCAAACCGCCCTCTCCGTCAGCCTCAACAAGTCCCGTCTCGCTGCAGCCTTCGTGTCCAACCTCGCTAAAGGCCCTGCAGTCAGAGGACTAGAGTACCAGGCCCTGAAAGACTGCATTGAGAACATGGGCGATACCGTGGACCGCCTCAGACAGTCGGTGAAAGAGCTCAGGGACTTGCGTCGAACAGCTGGTGGGGACTTGTTGTGGCACTTGAGTAATGTCCAGACTTGGGTCAGCGCCGCCCTGACTGATGAAACCACTTGCCTTGATGGGTTCGCCAGCCGCCGCTTGGATGGCCAAGTTAAGGCAGCGATTCGCCGGAAGATTACACTTGTTGCTCAAGTCACTAGTAATGCCCTCGCTCTTGTTAATCACTTCGCCGACAAAAAGCACTAA

mRNA sequence

ATGGCAAATCTCAAGATCTCACTCCCACTTCTCGTCCTTGTAGTCCTCCACAATGCCGCACCCACACACTCAGCCACCGCCACCAGCTTCATTGAGTCCTCCTGCAGAGTCACTCGCTACCCGGCGCTCTGCGTCCACTCCCTCTCAGCCTACGCCACTTCCATCCGCCAGAGCGGCCGTCAGCTCACCCAAACCGCCCTCTCCGTCAGCCTCAACAAGTCCCGTCTCGCTGCAGCCTTCGTGTCCAACCTCGCTAAAGGCCCTGCAGTCAGAGGACTAGAGTACCAGGCCCTGAAAGACTGCATTGAGAACATGGGCGATACCGTGGACCGCCTCAGACAGTCGGTGAAAGAGCTCAGGGACTTGCGTCGAACAGCTGGTGGGGACTTGTTGTGGCACTTGAGTAATGTCCAGACTTGGGTCAGCGCCGCCCTGACTGATGAAACCACTTGCCTTGATGGGTTCGCCAGCCGCCGCTTGGATGGCCAAGTTAAGGCAGCGATTCGCCGGAAGATTACACTTGTTGCTCAAGTCACTAGTAATGCCCTCGCTCTTGTTAATCACTTCGCCGACAAAAAGCACTAA

Coding sequence (CDS)

ATGGCAAATCTCAAGATCTCACTCCCACTTCTCGTCCTTGTAGTCCTCCACAATGCCGCACCCACACACTCAGCCACCGCCACCAGCTTCATTGAGTCCTCCTGCAGAGTCACTCGCTACCCGGCGCTCTGCGTCCACTCCCTCTCAGCCTACGCCACTTCCATCCGCCAGAGCGGCCGTCAGCTCACCCAAACCGCCCTCTCCGTCAGCCTCAACAAGTCCCGTCTCGCTGCAGCCTTCGTGTCCAACCTCGCTAAAGGCCCTGCAGTCAGAGGACTAGAGTACCAGGCCCTGAAAGACTGCATTGAGAACATGGGCGATACCGTGGACCGCCTCAGACAGTCGGTGAAAGAGCTCAGGGACTTGCGTCGAACAGCTGGTGGGGACTTGTTGTGGCACTTGAGTAATGTCCAGACTTGGGTCAGCGCCGCCCTGACTGATGAAACCACTTGCCTTGATGGGTTCGCCAGCCGCCGCTTGGATGGCCAAGTTAAGGCAGCGATTCGCCGGAAGATTACACTTGTTGCTCAAGTCACTAGTAATGCCCTCGCTCTTGTTAATCACTTCGCCGACAAAAAGCACTAA

Protein sequence

MANLKISLPLLVLVVLHNAAPTHSATATSFIESSCRVTRYPALCVHSLSAYATSIRQSGRQLTQTALSVSLNKSRLAAAFVSNLAKGPAVRGLEYQALKDCIENMGDTVDRLRQSVKELRDLRRTAGGDLLWHLSNVQTWVSAALTDETTCLDGFASRRLDGQVKAAIRRKITLVAQVTSNALALVNHFADKKH
Homology
[Close]
BLAST of Cp4.1LG05g02620 vs. Swiss-Prot
Match: 21KD_DAUCA (21 kDa protein OS=Daucus carota PE=2 SV=1)

HSP 1 Score: 171.4 bits (433), Expect = 9.7e-42
Identity = 88/186 (47.31%), Postives = 131/186 (70.43%), Query Frame = 1 

Query: 10  LLVLVVLHNAAPTHSATATSFIESSCRVTRYPALCVHSLSAYATSIRQSGRQLTQTALSV 69
           LLV++   +AAP     A  FI++SC +T YPA+C  SLSAYA +I+ + ++L  TAL V
Sbjct: 13  LLVILAAASAAP-----ANQFIKTSCTLTTYPAVCEQSLSAYAKTIQNNPQELASTALQV 72

Query: 70  SLNKSRLAAAFVSNLAKGPAVRGLEYQALKDCIENMGDTVDRLRQSVKELRDLRRTAGGD 129
           SL +++ A  F+  L K   ++  +Y A+ DC+E + D++DR+ +S  E+++L    G D
Sbjct: 73  SLTRTQQAQTFMKRLNKFKGLKARQYAAIHDCLEEVEDSLDRVSRSCDEMKNLSHAKGND 132

Query: 130 LLWHLSNVQTWVSAALTDETTCLDGFASRRLDGQVKAAIRRKITLVAQVTSNALALVNHF 189
             + +SNV+TWVSAALTDETTC+DGFA + +DG++K ++R ++  VA+VTSNALALVN+F
Sbjct: 133 FTFRMSNVETWVSAALTDETTCMDGFAGKGMDGKIKESVRAQVVAVARVTSNALALVNNF 192

Query: 190 ADK-KH 195
           A K KH
Sbjct: 193 AAKHKH 193

[Close]
BLAST of Cp4.1LG05g02620 vs. Swiss-Prot
Match: PME2_ARATH (Pectinesterase 2 OS=Arabidopsis thaliana GN=PME2 PE=2 SV=2)

HSP 1 Score: 76.6 bits (187), Expect = 3.2e-13
Identity = 49/171 (28.65%), Postives = 93/171 (54.39%), Query Frame = 1 

Query: 22  THSATATSFIESSCRVTRYPALCVHSLSAYATSIRQSGRQLTQTALSVSLNKSRLAAAFV 81
           T S+T+ + ++S C  T YP LC  +++A       S +++ + +L+++    +     V
Sbjct: 53  TLSSTSHAILKSVCSSTLYPELCFSAVAATGGKELTSQKEVIEASLNLTTKAVKHNYFAV 112

Query: 82  SNL-AKGPAVRGLEYQALKDCIENMGDTVDRLRQSVKELRDLRRTAGGDLLWHLSNVQTW 141
             L AK   +   E  AL DC+E + +T+D L  +V++L    +     L  H  +++T 
Sbjct: 113 KKLIAKRKGLTPREVTALHDCLETIDETLDELHVAVEDLHQYPKQKS--LRKHADDLKTL 172

Query: 142 VSAALTDETTCLDGFASRRLDGQVKAAIRRKITLVAQVTSNALALVNHFAD 192
           +S+A+T++ TCLDGF+    D +V+ A+ +    V  + SNALA++ +  +
Sbjct: 173 ISSAITNQGTCLDGFSYDDADRKVRKALLKGQVHVEHMCSNALAMIKNMTE 221

[Close]
BLAST of Cp4.1LG05g02620 vs. Swiss-Prot
Match: PME1_CITSI (Pectinesterase 1 OS=Citrus sinensis GN=PECS-1.1 PE=2 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 3.2e-13
Identity = 47/165 (28.48%), Postives = 90/165 (54.55%), Query Frame = 1 

Query: 29  SFIESSCRVTRYPALCVHSLSAYATSIRQSGRQLTQTALSVSLNKSRLAAAF--VSNLAK 88
           + ++SSC  TRYP LC  +++A   + ++   Q     +S+++  + +   +  +  L K
Sbjct: 67  AILKSSCSSTRYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLLK 126

Query: 89  GPAVRGLEYQALKDCIENMGDTVDRLRQSVKELRDLRRTAGGDLLWHLSNVQTWVSAALT 148
              +   E  AL DC+E + +T+D L ++V++L +        L  H  +++T +SAA+T
Sbjct: 127 RTNLTKREKVALHDCLETIDETLDELHKAVEDLEEYPNKKS--LSQHADDLKTLMSAAMT 186

Query: 149 DETTCLDGFASRRLDGQVKAAIRRKITLVAQVTSNALALVNHFAD 192
           ++ TCLDGF+    +  V+ A+      V ++ SNALA++ +  D
Sbjct: 187 NQGTCLDGFSHDDANKHVRDALSDGQVHVEKMCSNALAMIKNMTD 229

[Close]
BLAST of Cp4.1LG05g02620 vs. Swiss-Prot
Match: PME3_CITSI (Pectinesterase 3 OS=Citrus sinensis PE=1 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 3.2e-13
Identity = 47/165 (28.48%), Postives = 90/165 (54.55%), Query Frame = 1 

Query: 29  SFIESSCRVTRYPALCVHSLSAYATSIRQSGRQLTQTALSVSLNKSRLAAAF--VSNLAK 88
           + ++SSC  TRYP LC  +++A   + ++   Q     +S+++  + +   +  +  L K
Sbjct: 67  AILKSSCSSTRYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLLK 126

Query: 89  GPAVRGLEYQALKDCIENMGDTVDRLRQSVKELRDLRRTAGGDLLWHLSNVQTWVSAALT 148
              +   E  AL DC+E + +T+D L ++V++L +        L  H  +++T +SAA+T
Sbjct: 127 RTNLTKREKVALHDCLETIDETLDELHKAVEDLEEYPNKKS--LSQHADDLKTLMSAAMT 186

Query: 149 DETTCLDGFASRRLDGQVKAAIRRKITLVAQVTSNALALVNHFAD 192
           ++ TCLDGF+    +  V+ A+      V ++ SNALA++ +  D
Sbjct: 187 NQGTCLDGFSHDDANKHVRDALSDGQVHVEKMCSNALAMIKNMTD 229

[Close]
BLAST of Cp4.1LG05g02620 vs. Swiss-Prot
Match: PME3_ARATH (Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana GN=PME3 PE=2 SV=2)

HSP 1 Score: 76.3 bits (186), Expect = 4.2e-13
Identity = 53/179 (29.61%), Postives = 96/179 (53.63%), Query Frame = 1 

Query: 18  NAAPTHSATATSFIESSCRVTRYPALCVHSLSAYATSIRQSGRQLTQTALSVSLNKSRLA 77
           N   T S ++ + + SSC  TRYP LC+ ++         S + + + ++++++      
Sbjct: 46  NEKRTLSPSSHAVLRSSCSSTRYPELCISAVVTAGGVELTSQKDVIEASVNLTITAVEHN 105

Query: 78  AAFVSNLAKGPAVRGL---EYQALKDCIENMGDTVDRLRQSVKELR--DLRRTAGGDLLW 137
              V  L K    +GL   E  AL DC+E + +T+D L ++V++L     ++T    L  
Sbjct: 106 YFTVKKLIKKR--KGLTPREKTALHDCLETIDETLDELHETVEDLHLYPTKKT----LRE 165

Query: 138 HLSNVQTWVSAALTDETTCLDGFASRRLDGQVKAAIRRKITLVAQVTSNALALVNHFAD 192
           H  +++T +S+A+T++ TCLDGF+    D QV+ A+ +    V  + SNALA++ +  D
Sbjct: 166 HAGDLKTLISSAITNQETCLDGFSHDDADKQVRKALLKGQIHVEHMCSNALAMIKNMTD 218

[Close]
BLAST of Cp4.1LG05g02620 vs. TrEMBL
Match: A0A0A0KR60_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G160140 PE=4 SV=1)

HSP 1 Score: 295.4 bits (755), Expect = 5.0e-77
Identity = 156/195 (80.00%), Postives = 173/195 (88.72%), Query Frame = 1 

Query: 1   MANLKISLPLLV-LVVLHNAAPTHSATATSFIESSCRVTRYPALCVHSLSAYATSIRQSG 60
           MANLKISLPLL+ LV LHNAA T SA ATSFIESSC+VTRYPALCV SLS YA  IRQSG
Sbjct: 1   MANLKISLPLLITLVALHNAATTGSA-ATSFIESSCKVTRYPALCVQSLSTYANVIRQSG 60

Query: 61  RQLTQTALSVSLNKSRLAAAFVSNLAKGPAVRGLEYQALKDCIENMGDTVDRLRQSVKEL 120
           RQL +TALSVSL+K+RLA+AFV+ L KG  ++GLEYQA+KDCIENMGDTVDRL QSVKEL
Sbjct: 61  RQLARTALSVSLSKARLASAFVAKLGKGGGMKGLEYQAVKDCIENMGDTVDRLSQSVKEL 120

Query: 121 RDLRRTAGGDLLWHLSNVQTWVSAALTDETTCLDGFASRRLDGQVKAAIRRKITLVAQVT 180
            DLR+TAG D LWH++NVQTWVSAALTDETTCLDGFA RRLDGQ+KA IRR+ITLVAQ+T
Sbjct: 121 GDLRQTAGRDFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDGQIKAEIRRRITLVAQIT 180

Query: 181 SNALALVNHFADKKH 195
           SNALALVN FAD+ H
Sbjct: 181 SNALALVNRFADENH 194

[Close]
BLAST of Cp4.1LG05g02620 vs. TrEMBL
Match: F6HHR9_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_00s0323g00050 PE=4 SV=1)

HSP 1 Score: 212.2 bits (539), Expect = 5.5e-52
Identity = 116/197 (58.88%), Postives = 143/197 (72.59%), Query Frame = 1 

Query: 1   MANLKISLPLLVLVVLH-----NAAPTHSATATSFIESSCRVTRYPALCVHSLSAYATSI 60
           MA L +SL LLV  +LH     ++A   ++ ATSFI++SCRVTRYP LC   LS YA++I
Sbjct: 1   MAKLGLSL-LLVCSILHMVGTVDSAAARNSAATSFIKASCRVTRYPVLCFQCLSGYASTI 60

Query: 61  RQSGRQLTQTALSVSLNKSRLAAAFVSNLAKGPAVRGLEYQALKDCIENMGDTVDRLRQS 120
           RQS RQL  TALSVSL+++R A  F S L K   ++  E++A+KDCIENM DTVDRL  S
Sbjct: 61  RQSDRQLALTALSVSLSRARSATVFASKLTKVRGLKPREHEAVKDCIENMADTVDRLSHS 120

Query: 121 VKELRDLRRTAGGDLLWHLSNVQTWVSAALTDETTCLDGFASRRLDGQVKAAIRRKITLV 180
           V+EL    +    D +WH+SNVQTWVSAALTDE TCLDGFA R ++G VK A+RRK+  V
Sbjct: 121 VQELGRTGKAVSQDFMWHMSNVQTWVSAALTDENTCLDGFAGRVMEGNVKTAVRRKVIDV 180

Query: 181 AQVTSNALALVNHFADK 193
           AQVTSNALAL+N FA K
Sbjct: 181 AQVTSNALALINRFAAK 196

[Close]
BLAST of Cp4.1LG05g02620 vs. TrEMBL
Match: M5WCF4_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa015829mg PE=4 SV=1)

HSP 1 Score: 208.4 bits (529), Expect = 8.0e-51
Identity = 114/198 (57.58%), Postives = 142/198 (71.72%), Query Frame = 1 

Query: 1   MANLKISLPLLVLV-VLHNAAPTHSATA-----TSFIESSCRVTRYPALCVHSLSAYATS 60
           M  L + L  LVL  VLH      S  A     T+FI++SCR T YPALCV SL+ YA++
Sbjct: 1   MTRLGLGLSFLVLFHVLHMFGAADSTIARHSSPTAFIKASCRATDYPALCVQSLAGYASA 60

Query: 61  IRQSGRQLTQTALSVSLNKSRLAAAFVSNLAKGPAVRGLEYQALKDCIENMGDTVDRLRQ 120
           I QS RQL QTAL+VSL + R A ++V+ L +   ++  EY+A+KDCIENMGD+VDRLRQ
Sbjct: 61  IGQSERQLAQTALTVSLARVRSAQSYVAKLTRVRGIKPREYRAVKDCIENMGDSVDRLRQ 120

Query: 121 SVKELRDLRRTAGGDLLWHLSNVQTWVSAALTDETTCLDGFASRRLDGQVKAAIRRKITL 180
           SV+EL    R  G D +WH+SNV+TWVSAALTDE+TCLDGF+ R +DG +K AI R++  
Sbjct: 121 SVRELGQTGRAVGQDFMWHMSNVETWVSAALTDESTCLDGFSGRFMDGNIKTAITRRVNN 180

Query: 181 VAQVTSNALALVNHFADK 193
           VAQVTSNALALVN FA K
Sbjct: 181 VAQVTSNALALVNRFAAK 198

[Close]
BLAST of Cp4.1LG05g02620 vs. TrEMBL
Match: U5GJ63_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0003s11320g PE=4 SV=1)

HSP 1 Score: 207.6 bits (527), Expect = 1.4e-50
Identity = 112/192 (58.33%), Postives = 137/192 (71.35%), Query Frame = 1 

Query: 1   MANLKISLPLLVLVVLHNAAPTHSATATSFIESSCRVTRYPALCVHSLSAYATSIRQSGR 60
           MA L   L LL   +        +++ T FI+SSC+ TRYP LCV  LS YA+ I+++ R
Sbjct: 1   MAKLVSFLTLLSFALYMVGTAGSASSPTDFIKSSCKATRYPELCVGCLSGYASVIQRNER 60

Query: 61  QLTQTALSVSLNKSRLAAAFVSNLAKGPAVRGLEYQALKDCIENMGDTVDRLRQSVKELR 120
           +L  TALSVSL ++R AAAFV+ + K   ++  EYQA KDCIENMGD+VDRL QSV+ELR
Sbjct: 61  RLALTALSVSLARARSAAAFVTKMTKVRGIKPREYQAAKDCIENMGDSVDRLSQSVRELR 120

Query: 121 DLRRTAGGDLLWHLSNVQTWVSAALTDETTCLDGFASRRLDGQVKAAIRRKITLVAQVTS 180
              R  G D LWH+SNVQTWVSAALTDE TCLDGFA   +DG VK AI+R+I  VAQVTS
Sbjct: 121 HTGRAVGRDFLWHMSNVQTWVSAALTDENTCLDGFAGHLMDGNVKVAIKRRINNVAQVTS 180

Query: 181 NALALVNHFADK 193
           NALALV+ FA +
Sbjct: 181 NALALVDRFASR 192

[Close]
BLAST of Cp4.1LG05g02620 vs. TrEMBL
Match: A0A067JNQ8_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_20772 PE=4 SV=1)

HSP 1 Score: 206.5 bits (524), Expect = 3.0e-50
Identity = 107/192 (55.73%), Postives = 137/192 (71.35%), Query Frame = 1 

Query: 1   MANLKISLPLLVLVVLHNAAPTHSATATSFIESSCRVTRYPALCVHSLSAYATSIRQSGR 60
           MA   +SL LL   +         ++ T FI+SSC+ TRYP LCV  LS YA++I+Q+ +
Sbjct: 1   MARFVLSLILLSFALYMAGMIVSGSSPTDFIKSSCKATRYPDLCVECLSGYASAIQQNEK 60

Query: 61  QLTQTALSVSLNKSRLAAAFVSNLAKGPAVRGLEYQALKDCIENMGDTVDRLRQSVKELR 120
            L + ALSVSL +++ A  +V+ L K   ++  EYQA+KDCIENMGD+VDRL QS+KEL 
Sbjct: 61  HLAEVALSVSLARAKSATDYVTTLTKVRGIKAREYQAVKDCIENMGDSVDRLSQSIKELG 120

Query: 121 DLRRTAGGDLLWHLSNVQTWVSAALTDETTCLDGFASRRLDGQVKAAIRRKITLVAQVTS 180
            +    G D +WH+SNVQTWVSAALTDE TCLDGFA RR+DG VKAAI+ +IT V +VTS
Sbjct: 121 HMGGAVGQDFVWHMSNVQTWVSAALTDENTCLDGFAGRRMDGSVKAAIKHRITNVVKVTS 180

Query: 181 NALALVNHFADK 193
           NALALVN FA +
Sbjct: 181 NALALVNRFASR 192

[Close]
BLAST of Cp4.1LG05g02620 vs. TAIR10
Match: AT1G62770.1 (AT1G62770.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 184.1 bits (466), Expect = 8.1e-47
Identity = 104/188 (55.32%), Postives = 135/188 (71.81%), Query Frame = 1 

Query: 10  LLVLVVLHNAAPT-HSATATSFIESSCRVTRYPALCVHSLSAYATSIRQSGRQ-LTQTAL 69
           LL + +L +++ T + + +  FI SSC+ T+YP+LCVH+LSAYAT IR +  Q L QTAL
Sbjct: 12  LLSITILQSSSATPNRSESDQFIVSSCQTTQYPSLCVHTLSAYATKIRHNNDQDLAQTAL 71

Query: 70  SVSLNKSRLAAAFVSNLAKG-PAVRGLEYQALKDCIENMGDTVDRLRQSVKEL-RDLRRT 129
            +SL +++    FV+ L K  P  +  EY A+KDCIE +G++VDRL QSVKEL R     
Sbjct: 72  IISLARAKSVTIFVAKLTKETPKFKRREYLAIKDCIEVLGNSVDRLAQSVKELARAGHAV 131

Query: 130 AGGDLLWHLSNVQTWVSAALTDETTCLDGFASRRLDGQVKAAIRRKITLVAQVTSNALAL 189
           A  D +W +SNVQTWVSAALTDETTCLDGF+ R + G+VK  IR K+  VAQVTSNALAL
Sbjct: 132 ASEDFMWKMSNVQTWVSAALTDETTCLDGFSERAMGGKVKRLIRYKVVHVAQVTSNALAL 191

Query: 190 VNHFADKK 194
           VN FA+K+
Sbjct: 192 VNQFAEKR 199

[Close]
BLAST of Cp4.1LG05g02620 vs. TAIR10
Match: AT4G12390.1 (AT4G12390.1 pectin methylesterase inhibitor 1)

HSP 1 Score: 173.3 bits (438), Expect = 1.4e-43
Identity = 99/192 (51.56%), Postives = 132/192 (68.75%), Query Frame = 1 

Query: 4   LKISLPLLVLVVLHNAAPTHSATATS---FIESSCRVTRYPALCVHSLSAYATSIRQSGR 63
           L + LPLL    +  A P+ S   +S   FI SSCRVTRY  LCV  L+A+A  IR++  
Sbjct: 12  LLLFLPLLCQSTI--AKPSSSPNPSSSINFIVSSCRVTRYQTLCVKCLAAFADKIRRNEN 71

Query: 64  QLTQTALSVSLNKSRLAAAFVSNLAKGPAVRGLEYQALKDCIENMGDTVDRLRQSVKELR 123
           QL QTAL+V+L + +    +V  L K   ++  EY A+KDC+EN+GD ++ L QS++EL+
Sbjct: 72  QLAQTALAVTLVRVQSTTIYVGKLTKARRIKRREYLAVKDCVENLGDGLEMLAQSMRELK 131

Query: 124 DLRRTAGG--DLLWHLSNVQTWVSAALTDETTCLDGFASRRLDGQVKAAIRRKITLVAQV 183
            + R+     + LW LSNV+TWVSAALTDETTCLDGF  + +DG VK+AIRR++  VA+V
Sbjct: 132 QVGRSGRDRDEFLWRLSNVETWVSAALTDETTCLDGFDGKVMDGVVKSAIRRRVVHVARV 191

Query: 184 TSNALALVNHFA 191
           TSNALALVN FA
Sbjct: 192 TSNALALVNRFA 201

[Close]
BLAST of Cp4.1LG05g02620 vs. TAIR10
Match: AT5G62350.1 (AT5G62350.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 163.3 bits (412), Expect = 1.5e-40
Identity = 83/168 (49.40%), Postives = 117/168 (69.64%), Query Frame = 1 

Query: 24  SATATSFIESSCRVTRYPALCVHSLSAYATSIRQSGRQLTQTALSVSLNKSRLAAAFVSN 83
           S  A +FI+SSC+ T YPALCVHSLS YA  I+ S ++L +TA++V+L++++    FVS 
Sbjct: 32  SKKAINFIQSSCKTTTYPALCVHSLSVYANDIQTSPKRLAETAIAVTLSRAQSTKLFVSR 91

Query: 84  LAKGPAVRGLEYQALKDCIENMGDTVDRLRQSVKELRDLRRTAGGD-LLWHLSNVQTWVS 143
           L +   ++  E +A+KDC+E M DTVDRL +SV+EL+        D   +H+SN QTW S
Sbjct: 92  LTRMKGLKKREVEAIKDCVEEMNDTVDRLTKSVQELKLCGSAKDQDQFAYHMSNAQTWTS 151

Query: 144 AALTDETTCLDGFASRRLDGQVKAAIRRKITLVAQVTSNALALVNHFA 191
           AALTDE TC DGF+ R +DG++K ++R +I  V   TSNAL+L+N FA
Sbjct: 152 AALTDENTCSDGFSGRVMDGRIKNSVRARIMNVGHETSNALSLINAFA 199

[Close]
BLAST of Cp4.1LG05g02620 vs. TAIR10
Match: AT4G25260.1 (AT4G25260.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 161.4 bits (407), Expect = 5.6e-40
Identity = 88/197 (44.67%), Postives = 126/197 (63.96%), Query Frame = 1 

Query: 3   NLKISLPLLVL------VVLHNAAPTHSATATSFIESSCRVTRYPALCVHSLSAYATSIR 62
           N ++SL L VL      +V+       S+  T FI++SC  T YP  C  SLS+YA+ I+
Sbjct: 4   NFELSLILFVLYLSTAAIVMARNLEEESSGDTEFIKASCETTSYPDRCFQSLSSYASEIK 63

Query: 63  QSGRQLTQTALSVSLNKSRLAAAFVSNLAKGPAVRGLEYQALKDCIENMGDTVDRLRQSV 122
           +  R+L +TAL+VS+ +++ A  +VS +     +   +++A+ DC+E MGDTVDRL  S+
Sbjct: 64  KQPRKLAETALAVSIARAKSAKTYVSEMTDYKGITKRQHEAVADCLEEMGDTVDRLSNSL 123

Query: 123 KELRDLRRTAGGDLLWH-LSNVQTWVSAALTDETTCLDGFASRRLDGQVKAAIRRKITLV 182
           KEL+ L     G+  W  LSNV+TW SAALTDET C+DGF  + + G++K+ IR  I  V
Sbjct: 124 KELKHLEEGDSGEDFWFCLSNVRTWTSAALTDETACMDGFGGKAMAGELKSLIRTHIVSV 183

Query: 183 AQVTSNALALVNHFADK 193
           A+ TSNALAL+N FA K
Sbjct: 184 AEETSNALALINDFASK 200

[Close]
BLAST of Cp4.1LG05g02620 vs. TAIR10
Match: AT3G47380.1 (AT3G47380.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 154.8 bits (390), Expect = 5.3e-38
Identity = 80/170 (47.06%), Postives = 115/170 (67.65%), Query Frame = 1 

Query: 24  SATATSFIESSCRVTRYPALCVHSLSAYATSIRQSGRQLTQTALSVSLNKSRLAAAFVSN 83
           S  A +FI++SC+ T YP +CV+SL+ YA SI+ S R+L +TAL+V++ +++    FV  
Sbjct: 32  SKRAINFIQASCKATTYPTVCVNSLTGYANSIQTSPRRLAETALNVTVTQAQSTKVFVWR 91

Query: 84  LAKGPAVRGLEYQALKDCIENMGDTVDRLRQSVKELRDLRRTAGGDLLW-HLSNVQTWVS 143
           L +  +++  E QA+KDCIE + D VDRL  S+ E++      G D  W H+SN QTW S
Sbjct: 92  LGRFTSLKKREIQAVKDCIEEIHDAVDRLTMSIHEVKMCGSAKGRDQFWFHMSNAQTWTS 151

Query: 144 AALTDETTCLDGFASRRLDGQVKAAIRRKITLVAQVTSNALALVNHFADK 193
           AALT+  TC DGFA R +DG+VK ++R +I  + + TSNALAL+N FA K
Sbjct: 152 AALTNANTCSDGFAGRVMDGRVKNSVRARILNLGRGTSNALALINAFAKK 201

[Close]
BLAST of Cp4.1LG05g02620 vs. NCBI nr
Match: gi|449459700|ref|XP_004147584.1| (PREDICTED: 21 kDa protein [Cucumis sativus])

HSP 1 Score: 295.4 bits (755), Expect = 7.1e-77
Identity = 156/195 (80.00%), Postives = 173/195 (88.72%), Query Frame = 1 

Query: 1   MANLKISLPLLV-LVVLHNAAPTHSATATSFIESSCRVTRYPALCVHSLSAYATSIRQSG 60
           MANLKISLPLL+ LV LHNAA T SA ATSFIESSC+VTRYPALCV SLS YA  IRQSG
Sbjct: 1   MANLKISLPLLITLVALHNAATTGSA-ATSFIESSCKVTRYPALCVQSLSTYANVIRQSG 60

Query: 61  RQLTQTALSVSLNKSRLAAAFVSNLAKGPAVRGLEYQALKDCIENMGDTVDRLRQSVKEL 120
           RQL +TALSVSL+K+RLA+AFV+ L KG  ++GLEYQA+KDCIENMGDTVDRL QSVKEL
Sbjct: 61  RQLARTALSVSLSKARLASAFVAKLGKGGGMKGLEYQAVKDCIENMGDTVDRLSQSVKEL 120

Query: 121 RDLRRTAGGDLLWHLSNVQTWVSAALTDETTCLDGFASRRLDGQVKAAIRRKITLVAQVT 180
            DLR+TAG D LWH++NVQTWVSAALTDETTCLDGFA RRLDGQ+KA IRR+ITLVAQ+T
Sbjct: 121 GDLRQTAGRDFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDGQIKAEIRRRITLVAQIT 180

Query: 181 SNALALVNHFADKKH 195
           SNALALVN FAD+ H
Sbjct: 181 SNALALVNRFADENH 194

[Close]
BLAST of Cp4.1LG05g02620 vs. NCBI nr
Match: gi|659073572|ref|XP_008437134.1| (PREDICTED: 21 kDa protein-like [Cucumis melo])

HSP 1 Score: 290.8 bits (743), Expect = 1.7e-75
Identity = 153/195 (78.46%), Postives = 171/195 (87.69%), Query Frame = 1 

Query: 1   MANLKISLPLLV-LVVLHNAAPTHSATATSFIESSCRVTRYPALCVHSLSAYATSIRQSG 60
           MANLKISLPLL+ L+ LHNAA T S + TSFIESSC+VTRYPALCV SLS YA +IRQSG
Sbjct: 1   MANLKISLPLLITLIALHNAATTGSPS-TSFIESSCKVTRYPALCVQSLSTYANTIRQSG 60

Query: 61  RQLTQTALSVSLNKSRLAAAFVSNLAKGPAVRGLEYQALKDCIENMGDTVDRLRQSVKEL 120
           RQL +TALSVSL+K+ LAAAFV+ L KG  ++GLEYQA+KDCIENMGD+VDRL QSVKEL
Sbjct: 61  RQLARTALSVSLSKALLAAAFVAKLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSVKEL 120

Query: 121 RDLRRTAGGDLLWHLSNVQTWVSAALTDETTCLDGFASRRLDGQVKAAIRRKITLVAQVT 180
            DLRRTAG D LWH++NVQTWVSAALTDETTCLDGFA RRLDGQ KA IRR+ITLVAQ+T
Sbjct: 121 GDLRRTAGRDFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDGQTKAGIRRRITLVAQIT 180

Query: 181 SNALALVNHFADKKH 195
           SNALALVN FAD+ H
Sbjct: 181 SNALALVNRFADENH 194

[Close]
BLAST of Cp4.1LG05g02620 vs. NCBI nr
Match: gi|731438694|ref|XP_010646817.1| (PREDICTED: 21 kDa protein [Vitis vinifera])

HSP 1 Score: 212.2 bits (539), Expect = 7.9e-52
Identity = 116/197 (58.88%), Postives = 143/197 (72.59%), Query Frame = 1 

Query: 1   MANLKISLPLLVLVVLH-----NAAPTHSATATSFIESSCRVTRYPALCVHSLSAYATSI 60
           MA L +SL LLV  +LH     ++A   ++ ATSFI++SCRVTRYP LC   LS YA++I
Sbjct: 1   MAKLGLSL-LLVCSILHMVGTVDSAAARNSAATSFIKASCRVTRYPVLCFQCLSGYASTI 60

Query: 61  RQSGRQLTQTALSVSLNKSRLAAAFVSNLAKGPAVRGLEYQALKDCIENMGDTVDRLRQS 120
           RQS RQL  TALSVSL+++R A  F S L K   ++  E++A+KDCIENM DTVDRL  S
Sbjct: 61  RQSDRQLALTALSVSLSRARSATVFASKLTKVRGLKPREHEAVKDCIENMADTVDRLSHS 120

Query: 121 VKELRDLRRTAGGDLLWHLSNVQTWVSAALTDETTCLDGFASRRLDGQVKAAIRRKITLV 180
           V+EL    +    D +WH+SNVQTWVSAALTDE TCLDGFA R ++G VK A+RRK+  V
Sbjct: 121 VQELGRTGKAVSQDFMWHMSNVQTWVSAALTDENTCLDGFAGRVMEGNVKTAVRRKVIDV 180

Query: 181 AQVTSNALALVNHFADK 193
           AQVTSNALAL+N FA K
Sbjct: 181 AQVTSNALALINRFAAK 196

[Close]
BLAST of Cp4.1LG05g02620 vs. NCBI nr
Match: gi|470125365|ref|XP_004298673.1| (PREDICTED: 21 kDa protein [Fragaria vesca subsp. vesca])

HSP 1 Score: 210.3 bits (534), Expect = 3.0e-51
Identity = 108/190 (56.84%), Postives = 142/190 (74.74%), Query Frame = 1 

Query: 3   NLKISLPLLVLVVLHNAAPTHSATATSFIESSCRVTRYPALCVHSLSAYATSIRQSGRQL 62
           +L +   ++ LV   ++ P   +++T FI++SCR T+YPALCV  L+ YA SIRQS RQL
Sbjct: 8   SLLVLFHVVYLVGKADSTPPRHSSSTKFIKASCRATQYPALCVQCLAGYARSIRQSERQL 67

Query: 63  TQTALSVSLNKSRLAAAFVSNLAKGPAVRGLEYQALKDCIENMGDTVDRLRQSVKELRDL 122
            QTAL+VSL++ R A +FV+ L K   ++  EY+A+KDCIENMGD VD++ QS++EL   
Sbjct: 68  AQTALAVSLSRVRKAESFVTKLTKVRGIKPREYRAVKDCIENMGDGVDQIGQSMRELGHT 127

Query: 123 RRTAGGDLLWHLSNVQTWVSAALTDETTCLDGFASRRLDGQVKAAIRRKITLVAQVTSNA 182
            R  G + +WH+SNVQTWVSAALTDETTCLDGF+ R +DG +K AIRR++  VAQVTSNA
Sbjct: 128 GRAFGQEFMWHMSNVQTWVSAALTDETTCLDGFSGRLMDGNIKVAIRRRVNDVAQVTSNA 187

Query: 183 LALVNHFADK 193
           LALVN FA K
Sbjct: 188 LALVNRFATK 197

[Close]
BLAST of Cp4.1LG05g02620 vs. NCBI nr
Match: gi|720026234|ref|XP_010264193.1| (PREDICTED: 21 kDa protein-like [Nelumbo nucifera])

HSP 1 Score: 209.1 bits (531), Expect = 6.7e-51
Identity = 108/189 (57.14%), Postives = 142/189 (75.13%), Query Frame = 1 

Query: 5   KISLPLLVLVVLHNAAPT---HSATATSFIESSCRVTRYPALCVHSLSAYATSIRQSGRQ 64
           ++SL LL++ ++  AA T    S++ T FI++SC VTRYPA+CV SLS YA +I+QS RQ
Sbjct: 3   QVSLSLLLVSIIVYAAGTGRVESSSPTDFIKASCGVTRYPAVCVESLSVYANAIQQSPRQ 62

Query: 65  LTQTALSVSLNKSRLAAAFVSNLAKGPAVRGLEYQALKDCIENMGDTVDRLRQSVKELRD 124
           L Q AL VSL ++R A AFVS + +   ++G +YQA+KDC++N+GD+VDR+ +S+ EL  
Sbjct: 63  LAQAALQVSLGRARSATAFVSKMTRLRGLKGRQYQAVKDCVDNLGDSVDRISRSIHELGQ 122

Query: 125 LRRTAGGDLLWHLSNVQTWVSAALTDETTCLDGFASRRLDGQVKAAIRRKITLVAQVTSN 184
           + R A  D +WH+SNVQTWVSAALTDE TC+DGFA   +DG VK AIR KI  VAQVTSN
Sbjct: 123 INRGASQDFMWHMSNVQTWVSAALTDENTCMDGFAGHGMDGHVKTAIRGKILNVAQVTSN 182

Query: 185 ALALVNHFA 191
           ALAL+N FA
Sbjct: 183 ALALLNRFA 191

The following BLAST results are available for this feature:
BLAST of Cp4.1LG05g02620 vs. Swiss-Prot
Match NameE-valueIdentityDescription
21KD_DAUCA9.7e-4247.3121 kDa protein OS=Daucus carota PE=2 SV=1[more]
PME2_ARATH3.2e-1328.65Pectinesterase 2 OS=Arabidopsis thaliana GN=PME2 PE=2 SV=2[more]
PME1_CITSI3.2e-1328.48Pectinesterase 1 OS=Citrus sinensis GN=PECS-1.1 PE=2 SV=1[more]
PME3_CITSI3.2e-1328.48Pectinesterase 3 OS=Citrus sinensis PE=1 SV=1[more]
PME3_ARATH4.2e-1329.61Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana GN=PME3 PE=2 S... [more]
BLAST of Cp4.1LG05g02620 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KR60_CUCSA5.0e-7780.00Uncharacterized protein OS=Cucumis sativus GN=Csa_5G160140 PE=4 SV=1[more]
F6HHR9_VITVI5.5e-5258.88Putative uncharacterized protein OS=Vitis vinifera GN=VIT_00s0323g00050 PE=4 SV=... [more]
M5WCF4_PRUPE8.0e-5157.58Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa015829mg PE=4 SV=1[more]
U5GJ63_POPTR1.4e-5058.33Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0003s11320g PE=4 SV=1[more]
A0A067JNQ8_JATCU3.0e-5055.73Uncharacterized protein OS=Jatropha curcas GN=JCGZ_20772 PE=4 SV=1[more]
BLAST of Cp4.1LG05g02620 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G62770.18.1e-4755.32 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT4G12390.11.4e-4351.56 pectin methylesterase inhibitor 1[more]
AT5G62350.11.5e-4049.40 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT4G25260.15.6e-4044.67 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT3G47380.15.3e-3847.06 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
BLAST of Cp4.1LG05g02620 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|449459700|ref|XP_004147584.1|7.1e-7780.00PREDICTED: 21 kDa protein [Cucumis sativus][more]
gi|659073572|ref|XP_008437134.1|1.7e-7578.46PREDICTED: 21 kDa protein-like [Cucumis melo][more]
gi|731438694|ref|XP_010646817.1|7.9e-5258.88PREDICTED: 21 kDa protein [Vitis vinifera][more]
gi|470125365|ref|XP_004298673.1|3.0e-5156.84PREDICTED: 21 kDa protein [Fragaria vesca subsp. vesca][more]
gi|720026234|ref|XP_010264193.1|6.7e-5157.14PREDICTED: 21 kDa protein-like [Nelumbo nucifera][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0004857enzyme inhibitor activity
Vocabulary: INTERPRO
TermDefinition
IPR006501Pectinesterase_inhib_dom
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0005982 starch metabolic process
biological_process GO:0005985 sucrose metabolic process
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0004857 enzyme inhibitor activity
molecular_function GO:0030599 pectinesterase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG05g02620.1Cp4.1LG05g02620.1mRNA


InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainGENE3DG3DSA:1.20.140.40coord: 27..189
score: 7.1
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 29..185
score: 1.3
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 26..185
score: 1.5
IPR006501Pectinesterase inhibitor domainTIGRFAMsTIGR01614TIGR01614coord: 6..188
score: 6.4
IPR006501Pectinesterase inhibitor domainunknownSSF101148Plant invertase/pectin methylesterase inhibitorcoord: 29..189
score: 1.28
NoneNo IPR availableunknownCoilCoilcoord: 102..122
scor
NoneNo IPR availablePANTHERPTHR31080FAMILY NOT NAMEDcoord: 1..192
score: 8.6
NoneNo IPR availablePANTHERPTHR31080:SF2F23N19.14-RELATEDcoord: 1..192
score: 8.6

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Cp4.1LG05g02620Cucsa.251550Cucumber (Gy14) v1cgycpeB0686
Cp4.1LG05g02620Cucsa.302150Cucumber (Gy14) v1cgycpeB0792
Cp4.1LG05g02620CmaCh02G015240Cucurbita maxima (Rimu)cmacpeB647
Cp4.1LG05g02620CmoCh02G015680Cucurbita moschata (Rifu)cmocpeB596
Cp4.1LG05g02620Cla006265Watermelon (97103) v1cpewmB703
Cp4.1LG05g02620Cla011550Watermelon (97103) v1cpewmB719
Cp4.1LG05g02620Csa5G160140Cucumber (Chinese Long) v2cpecuB739
Cp4.1LG05g02620Csa3G176260Cucumber (Chinese Long) v2cpecuB719
Cp4.1LG05g02620MELO3C006821Melon (DHL92) v3.5.1cpemeB697
Cp4.1LG05g02620MELO3C005567Melon (DHL92) v3.5.1cpemeB647
Cp4.1LG05g02620ClCG01G003740Watermelon (Charleston Gray)cpewcgB639
Cp4.1LG05g02620ClCG05G007410Watermelon (Charleston Gray)cpewcgB676
Cp4.1LG05g02620CSPI05G06470Wild cucumber (PI 183967)cpecpiB742
Cp4.1LG05g02620CSPI03G16030Wild cucumber (PI 183967)cpecpiB721
Cp4.1LG05g02620Lsi09G003880Bottle gourd (USVL1VR-Ls)cpelsiB586
Cp4.1LG05g02620Lsi05G013170Bottle gourd (USVL1VR-Ls)cpelsiB624
Cp4.1LG05g02620MELO3C006821.2Melon (DHL92) v3.6.1cpemedB831
Cp4.1LG05g02620MELO3C005567.2Melon (DHL92) v3.6.1cpemedB774
Cp4.1LG05g02620CsaV3_5G003880Cucumber (Chinese Long) v3cpecucB0918
Cp4.1LG05g02620CsaV3_3G016210Cucumber (Chinese Long) v3cpecucB0896
Cp4.1LG05g02620CsGy3G016090Cucumber (Gy14) v2cgybcpeB427
Cp4.1LG05g02620CsGy5G003770Cucumber (Gy14) v2cgybcpeB694
Cp4.1LG05g02620Carg02463Silver-seed gourdcarcpeB1282
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Cp4.1LG05g02620Cucurbita pepo (Zucchini)cpecpeB226
Cp4.1LG05g02620Cucurbita pepo (Zucchini)cpecpeB508
Cp4.1LG05g02620Cucurbita maxima (Rimu)cmacpeB329
Cp4.1LG05g02620Cucurbita moschata (Rifu)cmocpeB292
Cp4.1LG05g02620Cucurbita moschata (Rifu)cmocpeB792