Cp4.1LG04g12860 (gene) Cucurbita pepo (Zucchini)

BLAST of Cp4.1LG04g12860 vs. Swiss-Prot
Match: GLR22_ARATH (Glutamate receptor 2.2 OS=Arabidopsis thaliana GN=GLR2.2 PE=2 SV=1)

HSP 1 Score: 618.2 bits (1593), Expect = 1.3e-175
Identity = 342/780 (43.85%), Postives = 491/780 (62.95%), Query Frame = 1

Query: 8   AVVDLVKNQKVHAIIGPESSSEATFMIKLGEKVRVPIISFSATSLSISPSHSPFFVRTAQ 67
           A +DL+KN++V AI+GP +S +A F+I++G+K RVP++S+SATS S++   SP+F R   
Sbjct: 87  AAIDLIKNKQVKAILGPWTSMQAHFLIEIGQKSRVPVVSYSATSPSLTSLRSPYFFRATY 146

Query: 68  NDSSQVKAITAIVQGFGWHELVLIYEDTEYGKGLIPFLTDELQQSNIRVPYKYAIATSMD 127
            DSSQV AI AI++ FGW E+V +Y D  +G+G++P LTD LQ  N+R+PY+  I  +  
Sbjct: 147 EDSSQVHAIKAIIKLFGWREVVPVYIDNTFGEGIMPRLTDSLQDINVRIPYRSVIPLNAT 206

Query: 128 AYQISEQLNMMKNRQTRVFLVHVTSPFGSVLFPLVDKAGMMSEGYAWILTNSLSNCLDAM 187
              IS +L  M N  TRVF+VH++S   S +F    + G+M  GY WILTN + + L ++
Sbjct: 207 DQDISVELLKMMNMPTRVFIVHMSSSLASTVFIKAKELGLMKPGYVWILTNGVMDGLRSI 266

Query: 188 DPLVIKSMEGVLGIRPYFPASEALESFKRRWKWSTP--ELNIYGLWAYDTIWALATAAER 247
           +   I++MEGVLGI+ Y P S+ LE+F+ RWK   P  ELN+YGLWAYD   ALA A E 
Sbjct: 267 NETGIEAMEGVLGIKTYIPKSKDLETFRSRWKRRFPQMELNVYGLWAYDATTALAMAIED 326

Query: 248 IGEGNNLRFLRGQGSDVE-GK--TDIANLGVSEVGPKLLKEMLNIKFQGLSGNFHLVNGH 307
            G  NN+ F     S+V+ GK  +++  LG+S+ GPKLL+ +  ++F+GL+G+FH V+G 
Sbjct: 327 AGI-NNMTF-----SNVDTGKNVSELDGLGLSQFGPKLLQTVSTVQFKGLAGDFHFVSGQ 386

Query: 308 LQPSAFEIFNLIGRGERLIGCWSPEKGICRNI-SDTKPTEKYSTSVSKLKKIIWPGDSIT 367
           LQPS FEI N+IG GER IG W+   G+ + +  + +     ST    LK IIWPG++++
Sbjct: 387 LQPSVFEIVNMIGTGERSIGFWTEGNGLVKKLDQEPRSIGTLSTWPDHLKHIIWPGEAVS 446

Query: 368 APKGWAVPANGEKIRIGVPKKQGFNEFLDVTRNPLTGELNFSGFCIDVFRAVADALPFPF 427
            PKGW +P NG+K+RIGVPK+ GF + + VTR+P+T      GFCID F AV  A+P+  
Sbjct: 447 VPKGWEIPTNGKKLRIGVPKRIGFTDLVKVTRDPITNSTVVKGFCIDFFEAVIQAMPYDV 506

Query: 428 PYEFELSRDEAGDSSVIYDDLLHQLNENEKKFDVVVGDITIVASRANYVDFSLPFTDSGV 487
            YEF       G+ +  ++DL+HQ+   +  FD VVGD TI+A+R+++VDF+LPF  SGV
Sbjct: 507 SYEFFPFEKPNGEPAGNHNDLVHQVYLGQ--FDAVVGDTTILANRSSFVDFTLPFMKSGV 566

Query: 488 TMLVPVKRNLHRSMWVFLKPLSLGLWLTTIAVSIATGAVLLILEHNGRSESLHPLNL-LC 547
            ++VP+K  + R  + FLKPLS+ LWLTT+      G  +  LEH   S+   P N    
Sbjct: 567 GLIVPLKDEVKRDKFSFLKPLSIELWLTTLVFFFLVGISVWTLEHRVNSDFRGPANYQAS 626

Query: 548 LILWFPVSSMVLPERQIVTNTRSRFVLVVWLFLAFVLMQSYTASLSSILMSDQLQPKYFS 607
            I WF  S+MV   R+ V +  +R ++V W F+  VL QSYTASL+S+L S QL P   S
Sbjct: 627 TIFWFAFSTMVFAPRERVLSFGARSLVVTWYFVLLVLTQSYTASLASLLTSQQLNPTITS 686

Query: 608 VSELISKGYYVGYQKGSFLKSMLIEQLKFNESKLKSYANVEEYRKALSKGSQNGGVAAIF 667
           +S L+ +G  VGYQ+ SF+   L  +  F +S L  +   EE  + L KG +NGGVAA F
Sbjct: 687 MSSLLHRGETVGYQRTSFILGKL-NETGFPQSSLVPFDTAEECDELLKKGPKNGGVAAAF 746

Query: 668 DEIPYLQVFLTKYGSDFTMAGPKYRTDGFGFAFPLNSRLVPYVSRAILNVTESEKMVAIQ 727
              PY+++FL +Y + + M    +  DGFGF FP+ S LV  VSRAIL V ES K V ++
Sbjct: 747 LGTPYVRLFLGQYCNTYKMVEEPFNVDGFGFVFPIGSPLVADVSRAILKVAESPKAVELE 806

Query: 728 TKYFGAGNQNEDSSISSPNS-PCLEA-----SSFGGLFIITGISLLLALIGSKT--FIWQ 773
             +F    Q+    +++P+S P + A      SF  LF++  +  +LAL G  T  F+W+
Sbjct: 807 HAWFKKKEQSCPDPVTNPDSNPTVTAIQLGVGSFWFLFLVVFVVCVLAL-GKFTFCFLWK 856

BLAST of Cp4.1LG04g12860 vs. Swiss-Prot
Match: GLR21_ARATH (Glutamate receptor 2.1 OS=Arabidopsis thaliana GN=GLR2.1 PE=2 SV=2)

HSP 1 Score: 581.3 bits (1497), Expect = 1.8e-164
Identity = 318/776 (40.98%), Postives = 480/776 (61.86%), Query Frame = 1

Query: 8   AVVDLVKNQKVHAIIGPESSSEATFMIKLGEKVRVPIISFSATSLSISPSHSPFFVRTAQ 67
           A +DL+ N++V AI+GP +S +A FMI++G+K +VPI+++SATS S++   S +F R   
Sbjct: 87  AALDLITNKEVKAILGPWTSMQAQFMIEMGQKSQVPIVTYSATSPSLASIRSQYFFRATY 146

Query: 68  NDSSQVKAITAIVQGFGWHELVLIYEDTEYGKGLIPFLTDELQQSNIRVPYKYAIATSMD 127
           +DSSQV AI  I++ FGW E+  +Y D  +G+G++P LTD LQ+ N+R+PY+  I+ +  
Sbjct: 147 DDSSQVHAIKEIIKLFGWREVAPVYVDDTFGEGIMPRLTDVLQEINVRIPYRTVISPNAT 206

Query: 128 AYQISEQLNMMKNRQTRVFLVHVTSPFGSVLFPLVDKAGMMSEGYAWILTNSLSNCLDAM 187
             +IS +L  M    TRVF+VH+     S  F    + G+M +GY WILTN++++ L  M
Sbjct: 207 DDEISVELLRMMTLPTRVFVVHLVELLASRFFAKATEIGLMKQGYVWILTNTITDVLSIM 266

Query: 188 DPLVIKSMEGVLGIRPYFPASEALESFKRRW--KWSTPELNIYGLWAYDTIWALATAAER 247
           +   I++M+GVLG++ Y P S+ LE+F+ RW  ++   +LN+YGLWAYD   ALA A E 
Sbjct: 267 NETEIETMQGVLGVKTYVPRSKELENFRSRWTKRFPISDLNVYGLWAYDATTALALAIEE 326

Query: 248 IGEGNNLRFLRGQGSDVEGKTDIANLGVSEVGPKLLKEMLNIKFQGLSGNFHLVNGHLQP 307
            G  +NL F++         +++  LGVS+ GPKLL+ +  ++FQGL+G+F  +NG LQP
Sbjct: 327 AGT-SNLTFVKMDAK--RNVSELQGLGVSQYGPKLLQTLSRVRFQGLAGDFQFINGELQP 386

Query: 308 SAFEIFNLIGRGERLIGCWSPEKGICRNISDTKPTEK--YSTSVSKLKKIIWPGDSITAP 367
           S FEI N+ G+G R IG W  E G+ +N+ D KP  K  +S+   +L+ IIWPGD+ + P
Sbjct: 387 SVFEIVNVNGQGGRTIGFWMKEYGLFKNV-DQKPASKTTFSSWQDRLRPIIWPGDTTSVP 446

Query: 368 KGWAVPANGEKIRIGVPKKQGFNEFLDVTRNPLTGELNFSGFCIDVFRAVADALPFPFPY 427
           KGW +P NG++++IGVP    F +F+  TR+P+T    FSGF ID F AV  A+P+   Y
Sbjct: 447 KGWEIPTNGKRLQIGVPVNNTFQQFVKATRDPITNSTIFSGFSIDYFEAVIQAIPYDISY 506

Query: 428 EFELSRDEAGDSSVIYDDLLHQLNENEKKFDVVVGDITIVASRANYVDFSLPFTDSGVTM 487
           +F   +D        YD L++Q+     K+D VV D TI ++R+ YVDFSLP+T SGV +
Sbjct: 507 DFIPFQDGG------YDALVYQVYLG--KYDAVVADTTISSNRSMYVDFSLPYTPSGVGL 566

Query: 488 LVPVKRNLHRSMWVFLKPLSLGLWLTTIAVSIATGAVLLILEHNGRSESLHPLNL-LCLI 547
           +VPVK ++ RS  +FL PL+L LWL ++      G V+ +LEH    +   P    L  I
Sbjct: 567 VVPVKDSVRRSSTIFLMPLTLALWLISLLSFFIIGLVVWVLEHRVNPDFDGPGQYQLSTI 626

Query: 548 LWFPVSSMVLPERQIVTNTRSRFVLVVWLFLAFVLMQSYTASLSSILMSDQLQPKYFSVS 607
            WF  S MV   R+ V +  +R V+++W FL  VL QSYTASL+S+L +  L P   +++
Sbjct: 627 FWFSFSIMVFAPRERVLSFWARVVVIIWYFLVLVLTQSYTASLASLLTTQHLHPTVTNIN 686

Query: 608 ELISKGYYVGYQKGSFLKSMLIEQLKFNESKLKSYANVEEYRKALSKGSQNGGVAAIFDE 667
            L++KG  VGYQ  SF+   L +   F+E+ L SY + E     LSKG   GGV+A+  E
Sbjct: 687 SLLAKGESVGYQ-SSFILGRLRDS-GFSEASLVSYGSPEHCDALLSKGQAEGGVSAVLME 746

Query: 668 IPYLQVFLTKYGSDFTMAGPKYRTDGFGFAFPLNSRLVPYVSRAILNVTESEKMVAIQTK 727
           +PY+++FL +Y + + M    ++ DG GF FP+ S LV  +SRAIL V ES K   ++  
Sbjct: 747 VPYVRIFLGQYCNKYKMVQTPFKVDGLGFVFPIGSPLVADISRAILKVEESNKANQLENA 806

Query: 728 YFGAGNQNEDSSISSPN-SPC-----LEASSFGGLFIITGISLLLALIGSKTFIWQ 773
           +F   +++    +++P+ +P      L   SF  LF++  I   +AL+    F++Q
Sbjct: 807 WFKPIDESCPDPLTNPDPNPSVSFRQLGFDSFWVLFLVAAIVCTMALL---KFVYQ 845

BLAST of Cp4.1LG04g12860 vs. Swiss-Prot
Match: GLR23_ARATH (Glutamate receptor 2.3 OS=Arabidopsis thaliana GN=GLR2.3 PE=2 SV=1)

HSP 1 Score: 575.1 bits (1481), Expect = 1.3e-162
Identity = 319/776 (41.11%), Postives = 470/776 (60.57%), Query Frame = 1

Query: 7   VAVVDLVKNQKVHAIIGPESSSEATFMIKLGEKVRVPIISFSATSLSISPSHSPFFVRTA 66
           +A +DL+KN++V AI+GP +S +A F+I++G+K RVPI+S+SATS  ++   SP+F+R  
Sbjct: 85  IAALDLIKNKQVKAILGPWTSMQAHFLIEIGQKSRVPIVSYSATSPILTSLRSPYFLRAT 144

Query: 67  QNDSSQVKAITAIVQGFGWHELVLIYEDTEYGKGLIPFLTDELQQSNIRVPYKYAIATSM 126
             DS QV+ I AI++ FGW E+V +Y D  +G+G++P LTD LQ  N+R+PY+  IA + 
Sbjct: 145 YEDSFQVQPIKAIIKLFGWREVVPVYIDNTFGEGIMPRLTDALQDINVRIPYRSVIAINA 204

Query: 127 DAYQISEQLNMMKNRQTRVFLVHVTSPFGSVLFPLVDKAGMMSEGYAWILTNSLSNCLDA 186
             ++IS +L  M N  TRVFLVH+     S  F    + G+M  GY WILTN + + L  
Sbjct: 205 TDHEISVELLKMMNMPTRVFLVHMYYDLASRFFIKAKELGLMEPGYVWILTNGVIDDLSL 264

Query: 187 MDPLVIKSMEGVLGIRPYFPASEALESFKRRWKWSTP--ELNIYGLWAYDTIWALATAAE 246
           ++   +++MEGVLGI+ Y P S  LE F+ RW+   P  EL++YGLWAYD   ALA A E
Sbjct: 265 INETAVEAMEGVLGIKTYIPKSPDLEKFRSRWRSLFPRVELSVYGLWAYDATTALAVAIE 324

Query: 247 RIGEGNNLRFLR--GQGSDVEGKTDIANLGVSEVGPKLLKEMLNIKFQGLSGNFHLVNGH 306
             G  NN+ F +    G +V   +++  LG+S+ GPKLL+ +L ++F+GL+G F    G 
Sbjct: 325 EAGT-NNMTFSKVVDTGRNV---SELEALGLSQFGPKLLQTLLTVQFRGLAGEFRFFRGQ 384

Query: 307 LQPSAFEIFNLIGRGERLIGCWSPEKGICRNI-SDTKPTEKYSTSVSKLKKIIWPGDSIT 366
           LQPS FEI N+I  GE+ IG W    G+ + +          ST    LK I+WPG++ +
Sbjct: 385 LQPSVFEIVNIINTGEKSIGFWKEGNGLVKKLDQQASSISALSTWKDHLKHIVWPGEADS 444

Query: 367 APKGWAVPANGEKIRIGVPKKQGFNEFLDVTRNPLTGELNFSGFCIDVFRAVADALPFPF 426
            PKGW +P  G+K+RIGVPK+ G+ + + VTR+P+T     +GFCID F AV   LP+  
Sbjct: 445 VPKGWQIPTKGKKLRIGVPKRTGYTDLVKVTRDPITNSTVVTGFCIDFFEAVIRELPYDV 504

Query: 427 PYEFELSRDEAGDSSVIYDDLLHQLNENEKKFDVVVGDITIVASRANYVDFSLPFTDSGV 486
            YEF       G ++  Y+DL++Q+     ++D VVGD TI+ +R++YVDF+ PF  SGV
Sbjct: 505 SYEFIPFEKPDGKTAGNYNDLVYQVYLG--RYDAVVGDTTILVNRSSYVDFTFPFIKSGV 564

Query: 487 TMLVPVKRNLHRSMWVFLKPLSLGLWLTTIAVSIATGAVLLILEHNGRSE-SLHPLNLLC 546
            ++V +   + R   +F+KPLS  LWLT+       G  + +LE+    + S  P     
Sbjct: 565 GLIVEMTDPVKRDYILFMKPLSWKLWLTSFISFFLVGCTVWVLEYKRNPDFSGPPRFQAS 624

Query: 547 LILWFPVSSMVLPERQIVTNTRSRFVLVVWLFLAFVLMQSYTASLSSILMSDQLQPKYFS 606
            I WF  S+MV   R+ V +  +R +++ W FL  VL QSYTASL+S+L S +L P   S
Sbjct: 625 TICWFAFSTMVFAPRERVFSFWARALVIAWYFLVLVLTQSYTASLASLLTSQKLNPTITS 684

Query: 607 VSELISKGYYVGYQKGSFLKSMLIEQLKFNESKLKSYANVEEYRKALSKGSQNGGVAAIF 666
           +S L+ KG  VGYQ+ SF+   L E+  F +S L  +   EE  + LSKG + GGV+  F
Sbjct: 685 MSSLLEKGETVGYQRTSFILGKLKER-GFPQSSLVPFDTAEECDELLSKGPKKGGVSGAF 744

Query: 667 DEIPYLQVFLTKYGSDFTMAGPKYRTDGFGFAFPLNSRLVPYVSRAILNVTESEKMVAIQ 726
            EIPYL++FL ++ + + M    +  DGFGF FP+ S LV  VSRAIL V ES K + ++
Sbjct: 745 LEIPYLRLFLGQFCNTYKMVEEPFNVDGFGFVFPIGSPLVADVSRAILKVAESPKAMELE 804

Query: 727 TKYFGAGNQNEDSSISSPN------SPCLEASSFGGLFIITGISLLLAL-IGSKTF 770
             +F    Q+    I++P+      S  L+  SF  LF+  G+ L+  + +G+ T+
Sbjct: 805 RAWFKKKEQSCPDPITNPDPNPSFTSRQLDIDSF--LFLFVGVLLVCVMALGNFTY 851

BLAST of Cp4.1LG04g12860 vs. Swiss-Prot
Match: GLR24_ARATH (Glutamate receptor 2.4 OS=Arabidopsis thaliana GN=GLR2.4 PE=2 SV=2)

HSP 1 Score: 575.1 bits (1481), Expect = 1.3e-162
Identity = 325/771 (42.15%), Postives = 474/771 (61.48%), Query Frame = 1

Query: 8   AVVDLVKNQKVHAIIGPESSSEATFMIKLGEKVRVPIISFSATSLSISPSHSPFFVRTAQ 67
           A +DL+KN++V AI+GP ++ +A+F+I++G+K +VPIISFSATS  +    SP+F R+  
Sbjct: 87  AALDLIKNKEVKAILGPRTTMQASFVIEVGQKSQVPIISFSATSPFLDSGRSPYFFRSTY 146

Query: 68  NDSSQVKAITAIVQGFGWHELVLIYEDTEYGKGLIPFLTDELQQSNIRVPYKYAIATSMD 127
           +DSSQV+AI+ I++ FGW E+V +YE+  +G+G++P LTD LQ  NIR+PY+  I+ +  
Sbjct: 147 DDSSQVQAISEIIKVFGWREVVPVYENNAFGEGIMPGLTDALQAINIRIPYRTVISPNAT 206

Query: 128 AYQISEQLNMMKNRQTRVFLVHVTSPFGSVLFPLVDKAGMMSEGYAWILTNSLSNCLDAM 187
             +IS  L  +  + TRVF+VH+     S +F    + G+M +GYAWILTN + + L  M
Sbjct: 207 DDEISVDLLKLMTKPTRVFVVHMNRFLASRVFSKARETGLMKQGYAWILTNGVIDHLVLM 266

Query: 188 DPLVIKSMEGVLGIRPYFPASEALESFKRRWKWSTP--ELNIYGLWAYDTIWALATAAER 247
           +   I++M+GV+GIR +FP SE L++F+ R   + P  ELNIYGL AYD   ALA A E 
Sbjct: 267 NGTDIEAMQGVIGIRTHFPISEELQTFRSRLAKAFPVSELNIYGLRAYDATTALAMAVEE 326

Query: 248 IGEGNNLRFLRGQGSDVEGKTDIANLGVSEVGPKLLKEMLNIKFQGLSGNFHLVNGHLQP 307
            G   NL F +  G ++   +D+  L VSE GPKL++ +  I+F+GLSG++H V+G L  
Sbjct: 327 AGT-TNLTFSKMDGRNI---SDLEALSVSEYGPKLIRSLSQIQFKGLSGDYHFVDGQLHA 386

Query: 308 SAFEIFNLIGRGERLIGCWSPEKGICRNISDTKPTEK-YSTSVSKLKKIIWPGDSITAPK 367
           S FEI N+I  G  L+G W+ +KG+ +++S +  T + +S+  + L  I+WPG ++T PK
Sbjct: 387 SVFEIVNVIDGGGILVGFWTQDKGLVKDLSPSSGTTRTFSSWKNHLNPILWPGITLTVPK 446

Query: 368 GWAVPANGEKIRIGVPKKQGFNEFLDVTRNPLTGELNFSGFCIDVFRAVADALPFPFPYE 427
           GW +P NG++++IGVP    F +F+ VT +PLT E   +GFCID F AV  A+P+   + 
Sbjct: 447 GWEIPTNGKELQIGVPVGT-FPQFVKVTTDPLTHETIVTGFCIDFFEAVIQAMPYDVSHR 506

Query: 428 FELSRDEAGDSSVIYDDLLHQLNENEKKFDVVVGDITIVASRANYVDFSLPFTDSGVTML 487
           F    D+ G ++V               FD VVGD TI+A+R++YVDF+LP+T SGV M+
Sbjct: 507 FIPFGDDDGKTNV---------------FDAVVGDTTILANRSSYVDFTLPYTTSGVGMV 566

Query: 488 VPVKRNLHRSMWVFLKPLSLGLWLTTIAVSIATGAVLLILEHNGRSESLHPLNL-LCLIL 547
           VP+K N+ RS  +F KPL+ GLW  T+      G V+ ILEH   SE   P    +  + 
Sbjct: 567 VPLKDNVARSSLIFFKPLTPGLWGMTLGSFFVVGFVVWILEHRVNSEFTGPPQYQISTMF 626

Query: 548 WFPVSSMVLPERQIVTNTRSRFVLVVWLFLAFVLMQSYTASLSSILMSDQLQPKYFSVSE 607
           WF  S MV   R+ V +  +R V++ W F+  VL QSYTASLSS+L + QL P   S+  
Sbjct: 627 WFAFSIMVFAPRERVMSFTARVVVITWYFIVLVLTQSYTASLSSLLTTQQLNPTETSIKN 686

Query: 608 LISKGYYVGYQKGSFLKSMLIEQLKFNESKLKSYANVEEYRKALSKGSQNGGVAAIFDEI 667
           +++KG  V YQ+ SF+   L E   F ES+L  + + E+  + L+KG   GGV+A F E+
Sbjct: 687 VLAKGGPVAYQRDSFVLGKLRES-GFPESRLVPFTSPEKCEELLNKGPSKGGVSAAFMEV 746

Query: 668 PYLQVFLTKYGSDFTMAGPKYRTDGFGFAFPLNSRLVPYVSRAILNVTESEKMVAIQTKY 727
           PY++VFL +Y   + M    +  DGFGF FP+ S LV  VSRAIL V ES K   ++T +
Sbjct: 747 PYVRVFLGQYCKKYKMVEVPFDVDGFGFVFPIGSPLVADVSRAILKVAESNKATQLETAW 806

Query: 728 FGAGNQNEDSSISSP-NSP---------CLEASSFGGLFIITGISLLLALI 765
           F    +N D +   P N+P          L   SF  LF+       LAL+
Sbjct: 807 F----KNIDKTCPDPMNNPDPNPTVSFRKLSLDSFLLLFVAAATVCTLALL 832

BLAST of Cp4.1LG04g12860 vs. Swiss-Prot
Match: GLR28_ARATH (Glutamate receptor 2.8 OS=Arabidopsis thaliana GN=GLR2.8 PE=2 SV=2)

HSP 1 Score: 573.2 bits (1476), Expect = 4.8e-162
Identity = 330/841 (39.24%), Postives = 488/841 (58.03%), Query Frame = 1

Query: 3   IRLMVAVVDLVKNQKVHAIIGPESSSEATFMIKLGEKVRVPIISFSATSLSISPSHSPFF 62
           ++   A +DL++N++V AIIGP  S +A FMIKL  K +VP ISFSATS  ++   S +F
Sbjct: 83  VQASAAALDLIQNEQVSAIIGPIDSMQAKFMIKLANKTQVPTISFSATSPLLTSIKSDYF 142

Query: 63  VRTAQNDSSQVKAITAIVQGFGWHELVLIYEDTEYGKGLIPFLTDELQQSNIRVPYKYAI 122
           VR   +DS QVKAI AI + FGW  +V IY D E G+G++P+L D LQ   +    +  I
Sbjct: 143 VRGTIDDSYQVKAIAAIFESFGWRSVVAIYVDNELGEGIMPYLFDALQDVQVD---RSVI 202

Query: 123 ATSMDAYQISEQLNMMKNRQTRVFLVHVTSPFGSVLFPLVDKAGMMSEGYAWILTNSLSN 182
            +  +  QI ++L  +  RQTRVF+VH+ S   S +F    + GMM EGY W++TN +++
Sbjct: 203 PSEANDDQILKELYKLMTRQTRVFVVHMASRLASRIFEKATEIGMMEEGYVWLMTNGMTH 262

Query: 183 CLDAM-DPLVIKSMEGVLGIRPYFPASEALESFKRRWK--------WSTPELNIYGLWAY 242
            +  +     + +++GVLG+R + P S+ LE F+ RWK        W   +L+I+GLWAY
Sbjct: 263 MMRHIHHGRSLNTIDGVLGVRSHVPKSKGLEDFRLRWKRNFKKENPWLRDDLSIFGLWAY 322

Query: 243 DTIWALATAAERIGEGNNLRFLRGQGSDVEGKTDIANLGVSEVGPKLLKEMLNIKFQGLS 302
           D+  ALA A E+    ++  +    GS     TD+  L VS  GP LL+ +  I+F GL+
Sbjct: 323 DSTTALAMAVEKTNI-SSFPYNNASGSS-NNMTDLGTLHVSRYGPSLLEALSEIRFNGLA 382

Query: 303 GNFHLVNGHLQPSAFEIFNLIGRGERLIGCWSPEKGICRNISDTKPTEKYSTSVSKLKKI 362
           G F+L++  L+   FEI N +G  ER++G W+P  G+  N++  K T   S +  +   +
Sbjct: 383 GRFNLIDRQLESPKFEIINFVGNEERIVGFWTPSNGLV-NVNSNKTT---SFTGERFGPL 442

Query: 363 IWPGDSITAPKGWAVPANGEKIRIGVPKKQGFNEFLDVTRNPLTGELNFSGFCIDVFRAV 422
           IWPG S   PKGW +P NG+KI++GVP K+GF  F++V  +P+T      G+ ID+F A 
Sbjct: 443 IWPGKSTIVPKGWEIPTNGKKIKVGVPVKKGFFNFVEVITDPITNITTPKGYAIDIFEAA 502

Query: 423 ADALPF---PFPYEFELSRDEAGDSSVIYDDLLHQLNENEKKFDVVVGDITIVASRANYV 482
              LP+   P  Y FE   D+       YDDL+++++      D VVGD+TI A R+ Y 
Sbjct: 503 LKKLPYSVIPQYYRFESPDDD-------YDDLVYKVDNG--TLDAVVGDVTITAYRSLYA 562

Query: 483 DFSLPFTDSGVTMLVPVKRNLHRSMWVFLKPLSLGLWLTTIAVSIATGAVLLILEHNGRS 542
           DF+LP+T+SGV+M+VPV+ N +++ WVFLKP  L LW+TT    +  G V+ + EH   +
Sbjct: 563 DFTLPYTESGVSMMVPVRDNENKNTWVFLKPWGLDLWVTTACFFVLIGFVVWLFEHRVNT 622

Query: 543 ESL-HPLNLLCLILWFPVSSMVLPERQIVTNTRSRFVLVVWLFLAFVLMQSYTASLSSIL 602
           +    P + +    WF  S+MV   R+ V +  +RFV+VVW F+  VL QSYTA+L+S L
Sbjct: 623 DFRGPPHHQIGTSFWFSFSTMVFAHREKVVSNLARFVVVVWCFVVLVLTQSYTANLTSFL 682

Query: 603 MSDQLQPKYFSVSELISKGYYVGYQKGSFLKSMLIEQLKFNESKLKSYANVEEYRKALSK 662
              + QP   +V +LI  G YVGYQ G+F+K  LI++  FN SKLK + + EE    LS 
Sbjct: 683 TVQRFQPAAINVKDLIKNGDYVGYQHGAFVKDFLIKE-GFNVSKLKPFGSSEECHALLS- 742

Query: 663 GSQNGGVAAIFDEIPYLQVFLTKYGSDFTMAGPKYRTDGFGFAFPLNSRLVPYVSRAILN 722
              NG ++A FDE+ YL+  L++Y S + +  P ++T GFGFAFP NS L   VS+AILN
Sbjct: 743 ---NGSISAAFDEVAYLRAILSQYCSKYAIVEPTFKTAGFGFAFPRNSPLTGDVSKAILN 802

Query: 723 VTESEKMVAIQTKYFGAGNQNEDSSISSPNSPCLEASSFGGLFIITGISLLLALI----- 782
           VT+ ++M  I+ K+F   N   D   ++ +S  L   SF GLF+I GI+  LAL+     
Sbjct: 803 VTQGDEMQHIENKWFMKQNDCPDPK-TALSSNRLSLRSFWGLFLIAGIASFLALLIFVFL 862

Query: 783 -----------GSKTFIWQKPASVAKTYYRKYVSFQQHLHSDVKDKVMDDRLKLPNTNTL 815
                       S+  IW+K  S+ + +  K +       S V          +P+ +TL
Sbjct: 863 FLYENRHTLCDDSEDSIWRKLTSLFRNFDEKDIKSHTFKSSAVHHVSSPMTQYIPSPSTL 899

BLAST of Cp4.1LG04g12860 vs. TrEMBL
Match: A0A0A0LX34_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G418780 PE=3 SV=1)

HSP 1 Score: 959.5 bits (2479), Expect = 2.6e-276
Identity = 468/548 (85.40%), Postives = 506/548 (92.34%), Query Frame = 1

Query: 8   AVVDLVKNQKVHAIIGPESSSEATFMIKLGEKVRVPIISFSATSLSISPSHSPFFVRTAQ 67
           AVVDLVKN+KVHAIIGPESS EATFMIKLGEK  VPI+SFSATSLSISPSHSPFFVRTAQ
Sbjct: 7   AVVDLVKNEKVHAIIGPESSGEATFMIKLGEKAHVPIVSFSATSLSISPSHSPFFVRTAQ 66

Query: 68  NDSSQVKAITAIVQGFGWHELVLIYEDTEYGKGLIPFLTDELQQSNIRVPYKYAIATSMD 127
           NDSSQV+AIT IVQGFGWH+LVLIYEDTEYG+GLIPFLTD LQ+SNIRV +KYAI TSMD
Sbjct: 67  NDSSQVQAITTIVQGFGWHDLVLIYEDTEYGRGLIPFLTDALQESNIRVSFKYAIPTSMD 126

Query: 128 AYQISEQLNMMKNRQTRVFLVHVTSPFGSVLFPLVDKAGMMSEGYAWILTNSLSNCLDAM 187
            Y+IS+ L+ MK RQTRVFLVHVTSPFGS LFPLV+KAGMM+EGYAW+LTN+LSNCLDAM
Sbjct: 127 PYEISQHLHKMKKRQTRVFLVHVTSPFGSALFPLVEKAGMMTEGYAWLLTNTLSNCLDAM 186

Query: 188 DPLVIKSMEGVLGIRPYFPASEALESFKRRWKWSTPELNIYGLWAYDTIWALATAAERIG 247
           DP VIKSMEGVLGIRP+FPASEALE+FKRRWKWS PELNIYGLWAYDTIWALA AAERIG
Sbjct: 187 DPSVIKSMEGVLGIRPHFPASEALENFKRRWKWSAPELNIYGLWAYDTIWALAMAAERIG 246

Query: 248 EGNNLRFLRGQGSDVEGKTDIANLGVSEVGPKLLKEMLNIKFQGLSGNFHLVNGHLQPSA 307
           + +NL FL+G+GSDVEGKTDIANL VSEVGP LLKEMLNIKF+GLSG+FHLVNGHLQPSA
Sbjct: 247 DVSNLGFLKGRGSDVEGKTDIANLRVSEVGPMLLKEMLNIKFKGLSGDFHLVNGHLQPSA 306

Query: 308 FEIFNLIGRGERLIGCWSPEKGICRNISDTKPTEKYSTSVSKLKKIIWPGDSITAPKGWA 367
           FEIFN+IGR ERLIG W+PE+GIC+NI++ KP EKYSTSVSKLKKIIWPGDSITAP+GWA
Sbjct: 307 FEIFNMIGRAERLIGYWNPEEGICQNIANKKPNEKYSTSVSKLKKIIWPGDSITAPRGWA 366

Query: 368 VPANGEKIRIGVPKKQGFNEFLDVTRNPLTGELNFSGFCIDVFRAVADALPFPFPYEFEL 427
           VPA+GEK RIGVPKKQGFNEFLDVTRNP TGELNF+GFCIDVFRAVADALPFP PYEFEL
Sbjct: 367 VPADGEKFRIGVPKKQGFNEFLDVTRNPQTGELNFTGFCIDVFRAVADALPFPLPYEFEL 426

Query: 428 SRDEAGDSSVIYDDLLHQLNENEK-KFDVVVGDITIVASRANYVDFSLPFTDSGVTMLVP 487
            +D+AGD+SVIYDDLLHQL E EK KFD VVGDITIVASRAN VDFSLP+TDSGVTMLVP
Sbjct: 427 FKDDAGDNSVIYDDLLHQLAEREKNKFDAVVGDITIVASRANLVDFSLPYTDSGVTMLVP 486

Query: 488 VKRNLHRSMWVFLKPLSLGLWLTTIAVSIATGAVLLILEHNGRSESLHPLNLLCLILWFP 547
           +K N+HRSMWVFLKPLSL LWLTTIA SIATG VLLILE N R ESL PL LLCLILWFP
Sbjct: 487 IKHNMHRSMWVFLKPLSLDLWLTTIAASIATGIVLLILEQNARRESLQPLELLCLILWFP 546

Query: 548 VSSMVLPE 555
            SS+VLPE
Sbjct: 547 FSSLVLPE 554

BLAST of Cp4.1LG04g12860 vs. TrEMBL
Match: A0A061E4Z0_THECC (Glutamate receptor OS=Theobroma cacao GN=TCM_009437 PE=3 SV=1)

HSP 1 Score: 782.7 bits (2020), Expect = 4.4e-223
Identity = 410/818 (50.12%), Postives = 566/818 (69.19%), Query Frame = 1

Query: 8   AVVDLVKNQKVHAIIGPESSSEATFMIKLGEKVRVPIISFSATSLSISPSHSPFFVRTAQ 67
           +VVDL+KN++ HAIIGP+ S+EA F I+LGE   VP++SFSATS S+SP+ + +F+RTAQ
Sbjct: 80  SVVDLLKNEQAHAIIGPQWSAEAKFAIELGEMAHVPMVSFSATSPSLSPTQNTYFIRTAQ 139

Query: 68  NDSSQVKAITAIVQGFGWHELVLIYEDTEYGKGLIPFLTDELQQSNIRVPYKYAIATSMD 127
           ND+SQ++AI A+++ FGW ++VLI+EDTEYG  ++P+L D  ++++I + YK  I++S  
Sbjct: 140 NDASQIRAIVAVLKQFGWPQVVLIHEDTEYGTEIVPYLFDSWRENDIGLAYKSKISSSAT 199

Query: 128 AYQISEQLNMMKNRQTRVFLVHVTSPFGSVLFPLVDKAGMMSEGYAWILTNSLSNCLDAM 187
            ++IS +LN ++  QT+VFLVH+TS  GS LF LVDKAGMMS GY+W++T+ +SN L+ M
Sbjct: 200 DFEISRELNKLRALQTKVFLVHMTSSLGSRLFSLVDKAGMMSIGYSWLITDGISNSLEDM 259

Query: 188 DPLVIKSMEGVLGIRPYFPASEALESFKRRWK---------WSTPELNIYGLWAYDTIWA 247
           DP VI SMEGVLG++P+ P +  +++FK+RW+          S  ELNI+G WAYDT+WA
Sbjct: 260 DPAVIHSMEGVLGVKPHVPETHVVKTFKKRWQRNLHLLKPNSSVGELNIFGFWAYDTVWA 319

Query: 248 LATAAERIGEGNNLRFLRGQGSDVEGKTDIANLGVSEVGPKLLKEMLNIKFQGLSGNFHL 307
           LATAAERI   N      G    V    D+ANL +S++GP+ L E+LN KF+GL G+FHL
Sbjct: 320 LATAAERIVPVNPTFLKVGTNGSV---MDLANLSISKIGPRFLSEILNTKFKGLGGDFHL 379

Query: 308 VNGHLQPSAFEIFNLIGRGERLIGCWSPEKGICRNISDTKPTEKYSTSVSKLKKIIWPGD 367
           V+G LQPS FEIFN+IGRG+R++G W+P++GI R +S T+       + S LK IIWPGD
Sbjct: 380 VDGQLQPSVFEIFNVIGRGQRIVGFWTPKEGISRALSSTE-----RVASSGLKNIIWPGD 439

Query: 368 SITAPKGWAVPANGEKIRIGVPKKQGFNEFLDVTRNPLTGELNFSGFCIDVFRAVADALP 427
           S   P GWA+P     +RIGVP K GF +F+ + +N   G +  +GF I+VF A    L 
Sbjct: 440 STEVPTGWAIP----MLRIGVPVKTGFTQFVKIDKNGADG-IRCTGFSIEVFEAALKKLA 499

Query: 428 FPFPYEFELSRDEAGDSSVIYDDLLHQLNENEKKFDVVVGDITIVASRANYVDFSLPFTD 487
           F   Y +    ++ G S+  Y+DLLHQ+    K  D VVGD TI+A R +YVDF+LP+T+
Sbjct: 500 FNVSYVYVPFMNDEGKSNGSYNDLLHQI--EHKIVDAVVGDTTIIAERTSYVDFTLPYTE 559

Query: 488 SGVTMLVPVKRNLHRSMWVFLKPLSLGLWLTTIAVSIATGAVLLILEHNGRSESLH---P 547
           S + M+VP+K    +S+W FL+PL   LW T I   I  G V+ I+E +  + +     P
Sbjct: 560 SRIVMVVPIKH--EKSLWSFLQPLGWDLWFTIIGSCIFFGLVIRIMERHHTANTRFGGPP 619

Query: 548 LNLLCLILWFPVSSMVLPERQIVTNTRSRFVLVVWLFLAFVLMQSYTASLSSILMSDQLQ 607
              L +I WFP SS+V P+R+++ N  S FVLV+WLFLA++LMQSYTASLSSIL  DQL 
Sbjct: 620 SRQLGMIFWFPFSSLVFPQRELLLNDYSIFVLVMWLFLAYILMQSYTASLSSILTVDQLH 679

Query: 608 PKYFSVSELISKGYYVGYQKGSFLKSMLIEQLKFNESKLKSYANVEEYRKALSKGSQNGG 667
           P +FSV  L +KGY VGYQ GSF+   L  +LKF+ESKLK+Y  +++Y KALS GS++ G
Sbjct: 680 PTFFSVQNLKTKGYNVGYQNGSFVLDFLKNRLKFDESKLKAYDTIQDYDKALSVGSEHDG 739

Query: 668 VAAIFDEIPYLQVFLTKYGSDFTMAGPKYRTDGFGFAFPLNSRLVPYVSRAILNVTESEK 727
           VAAIFDEIP++++FL KYGS++ + GP YRTDGFGFAFP  S LVPY+SRAIL V E   
Sbjct: 740 VAAIFDEIPFIRLFLDKYGSNYMITGPTYRTDGFGFAFPRGSPLVPYISRAILKVREDTI 799

Query: 728 MVAIQTKYFGAGNQNEDSSIS-SPNSPCLEASSFGGLFIITGISLLLALIGSKTFIWQKP 787
           M  I+ KYF     +  S+   S +S  L   SFGGLFIITGI+ LLAL+ S+ + W+KP
Sbjct: 800 MDNIEKKYFKHQVTSLYSAAPISADSRSLSLHSFGGLFIITGIATLLALVISEGYFWEKP 859

Query: 788 ASVAKTYYRKYVSFQQHLHSDVKDKVMDDRLKLPNTNT 813
            S+AK Y ++Y+S +    S+++ K++     + N +T
Sbjct: 860 VSMAKKYGQRYLSSRA---SNIETKLVAHSTTVMNAST 877

BLAST of Cp4.1LG04g12860 vs. TrEMBL
Match: B9IM39_POPTR (Glutamate receptor OS=Populus trichocarpa GN=POPTR_0018s10550g PE=3 SV=2)

HSP 1 Score: 740.3 bits (1910), Expect = 2.5e-210
Identity = 397/768 (51.69%), Postives = 525/768 (68.36%), Query Frame = 1

Query: 8   AVVDLVKNQKVHAIIGPESSSEATFMIKLGEKVRVPIISFSATSLSISPSHSPFFVRTAQ 67
           +V+DL+KN +VHAIIGP+ SS+A F+I+LG K  VPI+SFSATS ++S + S +FVRTAQ
Sbjct: 88  SVLDLMKNDQVHAIIGPQKSSQAKFVIELGGKAEVPIVSFSATSPTLSATQSKYFVRTAQ 147

Query: 68  NDSSQVKAITAIVQGFGWHELVLIYEDTEYGKGLIPFLTDELQQSNIRVPYKYAIATSMD 127
           +DSSQVKAI++IVQ +GW E+V IYEDTEYG GL+PFL D  Q+ + RVPY   I    +
Sbjct: 148 DDSSQVKAISSIVQAYGWREIVPIYEDTEYGNGLVPFLLDAFQEIDTRVPYGSRIPLYFN 207

Query: 128 AYQISEQLNMMKNRQTRVFLVHVTSPFGSVLFPLVDKAGMMSEGYAWILTNSLSNCLDAM 187
             QI  +L+ +K  +  +FLVH+++  GS LF L   AGMMSEGYAW++T  LS  LD +
Sbjct: 208 DTQIMSELHKLKEMKKSIFLVHMSASLGSRLFLLAKDAGMMSEGYAWLVTAGLSALLDPL 267

Query: 188 DPLVIKSMEGVLGIRPYFPASEALESFKRRWK----WSTP-----ELNIYGLWAYDTIWA 247
              V+ SM+GVLGI+P+ P+S+ LESFK RW+     S P     ELN++GLWAYDT+WA
Sbjct: 268 GSEVMDSMQGVLGIKPHIPSSKKLESFKSRWRKKFTISKPQSKINELNLFGLWAYDTVWA 327

Query: 248 LATAAERIGEGNNLRFLRGQGSDVEGKTDIANLGVSEVGPKLLKEMLNIKFQGLSGNFHL 307
           +A A E+ G  ++ R+++   S  E   DIA LG SE GP+LL  +L+ +FQGLSG+FHL
Sbjct: 328 IAMAVEKAGIVHS-RYVKPNTS--ESTVDIAALGKSETGPRLLSSILSTRFQGLSGDFHL 387

Query: 308 VNGHLQPSAFEIFNLIGRGERLIGCWSPEKGICRNISDTKPTEKYSTSVSKLKKIIWPGD 367
             G   PSAFEI NLIG+ ER+IG W+PE+G+ RN+  T     YSTS +KLK+ IWPGD
Sbjct: 388 AGGERVPSAFEILNLIGKAERVIGYWTPERGLSRNLY-TNGKIAYSTSKNKLKEPIWPGD 447

Query: 368 SITAPKGWAVPANGEKIRIGVPKKQGFNEFLDVTRNPLTGELNFSGFCIDVFRAVADALP 427
           +   PK         ++RIGVP K GFNEF+ V  NP   +   SGF  DVF +V +ALP
Sbjct: 448 TTQQPK---------RLRIGVPLKTGFNEFIKVEWNPEDDKPIVSGFTRDVFVSVVEALP 507

Query: 428 FPFPYEFELSRDEAGDSSVIYDDLLHQLNENEKKFDVVVGDITIVASRANYVDFSLPFTD 487
           FP PYEF    ++   S+  Y+DLL Q+    K FD  VGDITI+A+R+ YVDF+LPF++
Sbjct: 508 FPLPYEFIPFVNKNKQSAGTYNDLLDQI--KLKNFDAAVGDITIIANRSTYVDFTLPFSE 567

Query: 488 SGVTMLVPVKRNLHRSMWVFLKPLSLGLWLTTIAVSIATGAVLLILEH-NGRSESLHPLN 547
           SG+TM+V  KR+   +MW+FLKPLSL LWLTT    I TG V+ +LEH   +     P  
Sbjct: 568 SGITMVVLTKRDERENMWIFLKPLSLELWLTTGIAFILTGLVVWVLEHRENKVFRGKPAQ 627

Query: 548 LLCLILWFPVSSMVLPERQIVTNTRSRFVLVVWLFLAFVLMQSYTASLSSILMSDQLQPK 607
            L   LWF  S++    R+ V N  +RFVL++W+F+  ++ QSYTASL+SIL   +LQP 
Sbjct: 628 QLGTTLWFSFSTLFFAHREKVVNNWTRFVLIIWIFVVLIISQSYTASLASILTVKRLQPT 687

Query: 608 YFSVSELISKGYYVGYQKGSFLKSMLIEQLKFNESKLKSYANVEEYRKALSKGSQNGGVA 667
           +  V E+   GY+VG+QK SF+K  L++QL FN++ L+ Y+  EEY  ALS+G+ NGGVA
Sbjct: 688 FVDVKEIRKNGYFVGHQKDSFVKDFLVKQLNFNDTMLREYSTPEEYHDALSRGTHNGGVA 747

Query: 668 AIFDEIPYLQVFLTKYGSDFTMAGPKYRTDGFGFAFPLNSRLVPYVSRAILNVT-ESEKM 727
           AIF EIPY+++FL KY S F M GP Y+TDGFGFAFPL S LVPY+SRAILNVT + +KM
Sbjct: 748 AIFAEIPYIKLFLAKYCSKFQMVGPTYKTDGFGFAFPLGSPLVPYISRAILNVTQDKDKM 807

Query: 728 VAIQTKYFGAGNQNEDSSISSPNSPCLEASSFGGLFIITGISLLLALI 765
             I+ + FG      D +   P S  L   SFGGLFIITG++ + AL+
Sbjct: 808 DEIERRNFGGETTCSDQAAMVP-SGGLGLPSFGGLFIITGVASMSALL 839

BLAST of Cp4.1LG04g12860 vs. TrEMBL
Match: V4WIX7_9ROSI (Uncharacterized protein (Fragment) OS=Citrus clementina GN=CICLE_v10010672mg PE=3 SV=1)

HSP 1 Score: 724.5 bits (1869), Expect = 1.4e-205
Identity = 389/792 (49.12%), Postives = 528/792 (66.67%), Query Frame = 1

Query: 8   AVVDLVKNQKVHAIIGPESSSEATFMIKLGEKVRVPIISFSATSLSISPSHSPFFVRTAQ 67
           A VDL+K  KVHAIIGP+  + A F+++LGEK +VPIISF  TS ++SP+  PFF+R  Q
Sbjct: 94  AAVDLLKKFKVHAIIGPQIQAAAPFLVELGEKAQVPIISFFETSPALSPTEYPFFIRVTQ 153

Query: 68  NDSSQVKAITAIVQGFGWHELVLIYEDTEYGKGLIPFLTDELQQSNIRVPYKYAIATSMD 127
           NDS QVKAI+A++Q F WHE+VL+YEDT+YG G I FL DELQ+++IR+ +   I TS +
Sbjct: 154 NDSLQVKAISAVLQNFSWHEVVLMYEDTDYGAGFISFLVDELQENDIRISHMSKIPTSAE 213

Query: 128 AYQISEQLNMMKNRQTRVFLVHVTSPFGSVLFPLVDKAGMMSEGYAWILTNSLSNCLDAM 187
            +QIS++L+ +   QTRVF+VH+ +   S LF LVDK GMMSEGY WI+T   SN L+ +
Sbjct: 214 DFQISKELSKLSTMQTRVFIVHMNTALASRLFALVDKKGMMSEGYTWIVTARSSNSLNVL 273

Query: 188 DPLVIKSMEGVLGIRPYFPASEALESFKRRWK---------WSTPELNIYGLWAYDTIWA 247
           D  VI SMEGVLG+R + P S+ L  F RRWK          S  E+NI GLWAYDTI+A
Sbjct: 274 DSEVIDSMEGVLGVRSHLPKSKELGLFDRRWKSKLHSMKPNSSVTEINISGLWAYDTIFA 333

Query: 248 LATAAERIGEGNNLRFLRGQGSDVEGKTDIANLGVSEVGPKLLKEMLNIKFQGLSGNFHL 307
           LA A E+I    N   +    +  E  TD  +LG+S  GP L  ++LN +F+GLSG FHL
Sbjct: 334 LAKAVEKILSPTNPSIV-NPNNPSESTTDFGSLGISRTGPILNNQILNTQFKGLSGEFHL 393

Query: 308 VNGHLQPSAFEIFNLIGRGERLIGCWSPEKGICRNISDTKPTEKYSTSVSKLKKIIWPGD 367
           VNG L+   FEI N+IG G R++G W+ EKG+ + +         STS + LK+IIWPGD
Sbjct: 394 VNGQLESPVFEIVNVIGTG-RVVGYWTSEKGLTQTLD--------STSKNDLKRIIWPGD 453

Query: 368 SITAPKGWAVPANGEKIRIGVPKKQGFNEFLDVTRNPLTGELNFSGFCIDVFRAVADAL- 427
           S  AP GWA+P+    + +G P K GF EFL V ++    +  +SGFCID+F A  + + 
Sbjct: 454 STIAPTGWAIPS----LVVGTPVKLGFPEFLRVRKDDYLNKNIYSGFCIDIFNAALEIVE 513

Query: 428 --------PFPFPYEFELSRDEAGDSSVIYDDLLHQLNENEKKFDVVVGDITIVASRANY 487
                   P  FPYE     D  G+ +  YDDLL Q+  N +KFD VVGDI+IVASR +Y
Sbjct: 514 EKLGMKIHPQFFPYE-----DANGEMAGTYDDLLRQI--NVQKFDAVVGDISIVASRTDY 573

Query: 488 VDFSLPFTDSGVTMLVPVKRNLHRSMWVFLKPLSLGLWLTTIAVSIATGAVLLILEHNGR 547
           V+F+LP+++SGVTMLVPVKR+   +MW+FLKP +  LWLT     I    ++  +E    
Sbjct: 574 VEFTLPYSESGVTMLVPVKRDNRHNMWIFLKPWTWDLWLTVFVACIFIALIIRTMERQTE 633

Query: 548 SESL--HPLNLLCLILWFPVSSMVLPERQIVTNTRSRFVLVVWLFLAFVLMQSYTASLSS 607
           +      P   L +I  FP  +MV+P+R++V    S+FVLV+WL+LAF+LMQSYTASLSS
Sbjct: 634 NSEFAGSPRRQLGMIFMFPFYAMVIPQRELVVRDCSKFVLVIWLWLAFILMQSYTASLSS 693

Query: 608 ILMSDQLQPKYFSVSELISKGYYVGYQKGSFLKSMLIEQLKFNESKLKSYANVEEYRKAL 667
           IL  DQL+P +  + +L ++ ++VG+Q GSF+   L++QL F+ ++ +   N+ EY++AL
Sbjct: 694 ILTVDQLEPTFADLKKLRTESHFVGFQNGSFVGDFLVKQLNFSSNQTRPLINIGEYKEAL 753

Query: 668 SKGSQNGGVAAIFDEIPYLQVFLTKYGSDFTMAGPKYRTDGFGFAFPLNSRLVPYVSRAI 727
           SKGS+NGGV+AIF+EIPY++VFL KY S +T AGP YRTDG GFAF  +S L+ + S+AI
Sbjct: 754 SKGSRNGGVSAIFEEIPYIKVFLKKYSSKYTTAGPIYRTDGLGFAFARDSPLISHFSQAI 813

Query: 728 LNVTESE-KMVAIQTKYFGAGNQNEDSSIS-SPNSPCLEASSFGGLFIITGISLLLALIG 778
           L V E++ +M  ++ KYFG        + S S  S  L A +FGGLFII GI+ LLAL+ 
Sbjct: 814 LLVRENQTRMDRMEKKYFGENVMTPTLAPSISSESSSLRAYNFGGLFIIVGIATLLALLI 864

BLAST of Cp4.1LG04g12860 vs. TrEMBL
Match: A5AD54_VITVI (Glutamate receptor OS=Vitis vinifera GN=VITISV_032974 PE=3 SV=1)

HSP 1 Score: 715.3 bits (1845), Expect = 8.7e-203
Identity = 371/768 (48.31%), Postives = 515/768 (67.06%), Query Frame = 1

Query: 8   AVVDLVKNQKVHAIIGPESSSEATFMIKLGEKVRVPIISFSATSLSISPSHSPFFVRTAQ 67
           A +DL++N++V AIIGP SS +A F+I LG+K +VPIISFSA+S S+S   S +F+R   
Sbjct: 89  AALDLIQNEEVQAIIGPGSSMQANFLIVLGQKAQVPIISFSASSPSLSSLRSQYFIRATL 148

Query: 68  NDSSQVKAITAIVQGFGWHELVLIYEDTEYGKGLIPFLTDELQQSNIRVPYKYAIATSMD 127
           NDS+QV AI AI Q F W E VLIY D EYG G+IP++TD LQ  ++RV Y+  I+ S  
Sbjct: 149 NDSAQVPAIIAIFQAFEWREAVLIYVDNEYGDGIIPYMTDALQGIDVRVTYRSVISPSAT 208

Query: 128 AYQISEQLNMMKNRQTRVFLVHVTSPFGSVLFPLVDKAGMMSEGYAWILTNSLSNCLDAM 187
             QI E+L  +   QTRVF+VH+ +P GS  F   D+ GMM EGY WILT+ L++ L  +
Sbjct: 209 DDQIGEELYKLMTMQTRVFIVHMVTPLGSRFFTKADEIGMMEEGYVWILTDGLTDLLSTL 268

Query: 188 DPLVIKSMEGVLGIRPYFPASEALESFKRRWKW---------STPELNIYGLWAYDTIWA 247
           DPLVI SM+GVLGI+P+ P ++ LE+F+ RWK           T ELNI+GLWAYD   A
Sbjct: 269 DPLVIDSMQGVLGIKPHVPRTKELENFRVRWKRKFQQDHPKDETSELNIFGLWAYDAASA 328

Query: 248 LATAAERIGEGNNLRFLRGQGSDVEGKTDIANLGVSEVGPKLLKEMLNIKFQGLSGNFHL 307
           LA A E++G   NL F +   S     TD+  +GVS++GPKLL+ +L+ KF+GLSG+F +
Sbjct: 329 LAMAVEKVG-ATNLSFQKTNIS--SNSTDLDTIGVSQIGPKLLQSLLSTKFKGLSGDFQI 388

Query: 308 VNGHLQPSAFEIFNLIGRGERLIGCWSPEKGICRNISDTKP-TEKYSTSVSKLKKIIWPG 367
            +G L P+AF+I N+IG+GER IG W+P+ GI R +  T   +  YSTS   L  I+WPG
Sbjct: 389 FDGQLHPTAFQIVNVIGKGERGIGFWTPKNGIIRRLKFTNANSNTYSTSKDNLGAIVWPG 448

Query: 368 DSITAPKGWAVPANGEKIRIGVPKKQGFNEFLDVTRNPLTGELNFSGFCIDVFRAVADAL 427
           +    PKGW +P N +K++IGVP K GF+EF+ VT +P T     +G+CIDVF AV  +L
Sbjct: 449 EPTYFPKGWVLPVNEKKLKIGVPVKDGFSEFVKVTWDPNTNATKVAGYCIDVFDAVMSSL 508

Query: 428 PFPFPYEFELSRDEAGDSSVIYDDLLHQLNENEKKFDVVVGDITIVASRANYVDFSLPFT 487
           P+  PYE+       G  +  Y+DLL+Q+    KK+D VVGD TIVA+R+NYVDF+LP+T
Sbjct: 509 PYAVPYEYIPFGTPDGKPAGNYNDLLYQV--FLKKYDAVVGDTTIVANRSNYVDFTLPYT 568

Query: 488 DSGVTMLVPVKRNLHRSMWVFLKPLSLGLWLTTIAVSIATGAVLLILEHNGRSESL-HPL 547
           +SGV+M+VP+K N  +S W+FLKPL+ GLW+T+    +  G V+ +LEH    +    P 
Sbjct: 569 ESGVSMIVPIKDNKSKSAWIFLKPLTWGLWVTSACFFVFIGFVIWVLEHRINEDFRGPPS 628

Query: 548 NLLCLILWFPVSSMVLPERQIVTNTRSRFVLVVWLFLAFVLMQSYTASLSSILMSDQLQP 607
           +    I WF  S+MV  +++ + +  +RFV+++W F+  +L QSYTASL+S+L   QLQP
Sbjct: 629 HQAGTIFWFSFSTMVFAQKERIVSNLARFVMIIWFFVVLILTQSYTASLTSMLTVQQLQP 688

Query: 608 KYFSVSELISKGYYVGYQKGSFLKSMLIEQLKFNESKLKSYANVEEYRKALSKGSQNGGV 667
               + EL +KG YVGYQ+GSF+   L +++ F+ESK + Y + E   + LSKGS NGG+
Sbjct: 689 TVTDIKELRAKGEYVGYQQGSFVLGFL-KRMNFDESKFRIYNSSENLAELLSKGSANGGI 748

Query: 668 AAIFDEIPYLQVFLTKYGSDFTMAGPKYRTDGFGFAFPLNSRLVPYVSRAILNVTESEKM 727
           AA FDEIPY+++F+ ++ S +TM  P Y+ DGFGFAFP  S LV  VSRA+LNVTE ++M
Sbjct: 749 AAAFDEIPYMKLFIAQHCSKYTMVQPTYKYDGFGFAFPRGSPLVQDVSRAVLNVTEGDEM 808

Query: 728 VAIQTKYFGAGNQNEDSSISSPNSPCLEASSFGGLFIITGISLLLALI 765
           V I+ ++FG      D + SS +S  +   SF GLF+I G++  LALI
Sbjct: 809 VKIEKEWFGKKTSCSDDNGSSISSNNISLDSFWGLFLIAGVTSSLALI 850

BLAST of Cp4.1LG04g12860 vs. TAIR10
Match: AT2G24720.1 (AT2G24720.1 glutamate receptor 2.2)

HSP 1 Score: 618.2 bits (1593), Expect = 7.3e-177
Identity = 342/780 (43.85%), Postives = 491/780 (62.95%), Query Frame = 1

Query: 8   AVVDLVKNQKVHAIIGPESSSEATFMIKLGEKVRVPIISFSATSLSISPSHSPFFVRTAQ 67
           A +DL+KN++V AI+GP +S +A F+I++G+K RVP++S+SATS S++   SP+F R   
Sbjct: 87  AAIDLIKNKQVKAILGPWTSMQAHFLIEIGQKSRVPVVSYSATSPSLTSLRSPYFFRATY 146

Query: 68  NDSSQVKAITAIVQGFGWHELVLIYEDTEYGKGLIPFLTDELQQSNIRVPYKYAIATSMD 127
            DSSQV AI AI++ FGW E+V +Y D  +G+G++P LTD LQ  N+R+PY+  I  +  
Sbjct: 147 EDSSQVHAIKAIIKLFGWREVVPVYIDNTFGEGIMPRLTDSLQDINVRIPYRSVIPLNAT 206

Query: 128 AYQISEQLNMMKNRQTRVFLVHVTSPFGSVLFPLVDKAGMMSEGYAWILTNSLSNCLDAM 187
              IS +L  M N  TRVF+VH++S   S +F    + G+M  GY WILTN + + L ++
Sbjct: 207 DQDISVELLKMMNMPTRVFIVHMSSSLASTVFIKAKELGLMKPGYVWILTNGVMDGLRSI 266

Query: 188 DPLVIKSMEGVLGIRPYFPASEALESFKRRWKWSTP--ELNIYGLWAYDTIWALATAAER 247
           +   I++MEGVLGI+ Y P S+ LE+F+ RWK   P  ELN+YGLWAYD   ALA A E 
Sbjct: 267 NETGIEAMEGVLGIKTYIPKSKDLETFRSRWKRRFPQMELNVYGLWAYDATTALAMAIED 326

Query: 248 IGEGNNLRFLRGQGSDVE-GK--TDIANLGVSEVGPKLLKEMLNIKFQGLSGNFHLVNGH 307
            G  NN+ F     S+V+ GK  +++  LG+S+ GPKLL+ +  ++F+GL+G+FH V+G 
Sbjct: 327 AGI-NNMTF-----SNVDTGKNVSELDGLGLSQFGPKLLQTVSTVQFKGLAGDFHFVSGQ 386

Query: 308 LQPSAFEIFNLIGRGERLIGCWSPEKGICRNI-SDTKPTEKYSTSVSKLKKIIWPGDSIT 367
           LQPS FEI N+IG GER IG W+   G+ + +  + +     ST    LK IIWPG++++
Sbjct: 387 LQPSVFEIVNMIGTGERSIGFWTEGNGLVKKLDQEPRSIGTLSTWPDHLKHIIWPGEAVS 446

Query: 368 APKGWAVPANGEKIRIGVPKKQGFNEFLDVTRNPLTGELNFSGFCIDVFRAVADALPFPF 427
            PKGW +P NG+K+RIGVPK+ GF + + VTR+P+T      GFCID F AV  A+P+  
Sbjct: 447 VPKGWEIPTNGKKLRIGVPKRIGFTDLVKVTRDPITNSTVVKGFCIDFFEAVIQAMPYDV 506

Query: 428 PYEFELSRDEAGDSSVIYDDLLHQLNENEKKFDVVVGDITIVASRANYVDFSLPFTDSGV 487
            YEF       G+ +  ++DL+HQ+   +  FD VVGD TI+A+R+++VDF+LPF  SGV
Sbjct: 507 SYEFFPFEKPNGEPAGNHNDLVHQVYLGQ--FDAVVGDTTILANRSSFVDFTLPFMKSGV 566

Query: 488 TMLVPVKRNLHRSMWVFLKPLSLGLWLTTIAVSIATGAVLLILEHNGRSESLHPLNL-LC 547
            ++VP+K  + R  + FLKPLS+ LWLTT+      G  +  LEH   S+   P N    
Sbjct: 567 GLIVPLKDEVKRDKFSFLKPLSIELWLTTLVFFFLVGISVWTLEHRVNSDFRGPANYQAS 626

Query: 548 LILWFPVSSMVLPERQIVTNTRSRFVLVVWLFLAFVLMQSYTASLSSILMSDQLQPKYFS 607
            I WF  S+MV   R+ V +  +R ++V W F+  VL QSYTASL+S+L S QL P   S
Sbjct: 627 TIFWFAFSTMVFAPRERVLSFGARSLVVTWYFVLLVLTQSYTASLASLLTSQQLNPTITS 686

Query: 608 VSELISKGYYVGYQKGSFLKSMLIEQLKFNESKLKSYANVEEYRKALSKGSQNGGVAAIF 667
           +S L+ +G  VGYQ+ SF+   L  +  F +S L  +   EE  + L KG +NGGVAA F
Sbjct: 687 MSSLLHRGETVGYQRTSFILGKL-NETGFPQSSLVPFDTAEECDELLKKGPKNGGVAAAF 746

Query: 668 DEIPYLQVFLTKYGSDFTMAGPKYRTDGFGFAFPLNSRLVPYVSRAILNVTESEKMVAIQ 727
              PY+++FL +Y + + M    +  DGFGF FP+ S LV  VSRAIL V ES K V ++
Sbjct: 747 LGTPYVRLFLGQYCNTYKMVEEPFNVDGFGFVFPIGSPLVADVSRAILKVAESPKAVELE 806

Query: 728 TKYFGAGNQNEDSSISSPNS-PCLEA-----SSFGGLFIITGISLLLALIGSKT--FIWQ 773
             +F    Q+    +++P+S P + A      SF  LF++  +  +LAL G  T  F+W+
Sbjct: 807 HAWFKKKEQSCPDPVTNPDSNPTVTAIQLGVGSFWFLFLVVFVVCVLAL-GKFTFCFLWK 856

BLAST of Cp4.1LG04g12860 vs. TAIR10
Match: AT5G27100.1 (AT5G27100.1 glutamate receptor 2.1)

HSP 1 Score: 581.3 bits (1497), Expect = 9.9e-166
Identity = 318/776 (40.98%), Postives = 480/776 (61.86%), Query Frame = 1

Query: 8   AVVDLVKNQKVHAIIGPESSSEATFMIKLGEKVRVPIISFSATSLSISPSHSPFFVRTAQ 67
           A +DL+ N++V AI+GP +S +A FMI++G+K +VPI+++SATS S++   S +F R   
Sbjct: 87  AALDLITNKEVKAILGPWTSMQAQFMIEMGQKSQVPIVTYSATSPSLASIRSQYFFRATY 146

Query: 68  NDSSQVKAITAIVQGFGWHELVLIYEDTEYGKGLIPFLTDELQQSNIRVPYKYAIATSMD 127
           +DSSQV AI  I++ FGW E+  +Y D  +G+G++P LTD LQ+ N+R+PY+  I+ +  
Sbjct: 147 DDSSQVHAIKEIIKLFGWREVAPVYVDDTFGEGIMPRLTDVLQEINVRIPYRTVISPNAT 206

Query: 128 AYQISEQLNMMKNRQTRVFLVHVTSPFGSVLFPLVDKAGMMSEGYAWILTNSLSNCLDAM 187
             +IS +L  M    TRVF+VH+     S  F    + G+M +GY WILTN++++ L  M
Sbjct: 207 DDEISVELLRMMTLPTRVFVVHLVELLASRFFAKATEIGLMKQGYVWILTNTITDVLSIM 266

Query: 188 DPLVIKSMEGVLGIRPYFPASEALESFKRRW--KWSTPELNIYGLWAYDTIWALATAAER 247
           +   I++M+GVLG++ Y P S+ LE+F+ RW  ++   +LN+YGLWAYD   ALA A E 
Sbjct: 267 NETEIETMQGVLGVKTYVPRSKELENFRSRWTKRFPISDLNVYGLWAYDATTALALAIEE 326

Query: 248 IGEGNNLRFLRGQGSDVEGKTDIANLGVSEVGPKLLKEMLNIKFQGLSGNFHLVNGHLQP 307
            G  +NL F++         +++  LGVS+ GPKLL+ +  ++FQGL+G+F  +NG LQP
Sbjct: 327 AGT-SNLTFVKMDAK--RNVSELQGLGVSQYGPKLLQTLSRVRFQGLAGDFQFINGELQP 386

Query: 308 SAFEIFNLIGRGERLIGCWSPEKGICRNISDTKPTEK--YSTSVSKLKKIIWPGDSITAP 367
           S FEI N+ G+G R IG W  E G+ +N+ D KP  K  +S+   +L+ IIWPGD+ + P
Sbjct: 387 SVFEIVNVNGQGGRTIGFWMKEYGLFKNV-DQKPASKTTFSSWQDRLRPIIWPGDTTSVP 446

Query: 368 KGWAVPANGEKIRIGVPKKQGFNEFLDVTRNPLTGELNFSGFCIDVFRAVADALPFPFPY 427
           KGW +P NG++++IGVP    F +F+  TR+P+T    FSGF ID F AV  A+P+   Y
Sbjct: 447 KGWEIPTNGKRLQIGVPVNNTFQQFVKATRDPITNSTIFSGFSIDYFEAVIQAIPYDISY 506

Query: 428 EFELSRDEAGDSSVIYDDLLHQLNENEKKFDVVVGDITIVASRANYVDFSLPFTDSGVTM 487
           +F   +D        YD L++Q+     K+D VV D TI ++R+ YVDFSLP+T SGV +
Sbjct: 507 DFIPFQDGG------YDALVYQVYLG--KYDAVVADTTISSNRSMYVDFSLPYTPSGVGL 566

Query: 488 LVPVKRNLHRSMWVFLKPLSLGLWLTTIAVSIATGAVLLILEHNGRSESLHPLNL-LCLI 547
           +VPVK ++ RS  +FL PL+L LWL ++      G V+ +LEH    +   P    L  I
Sbjct: 567 VVPVKDSVRRSSTIFLMPLTLALWLISLLSFFIIGLVVWVLEHRVNPDFDGPGQYQLSTI 626

Query: 548 LWFPVSSMVLPERQIVTNTRSRFVLVVWLFLAFVLMQSYTASLSSILMSDQLQPKYFSVS 607
            WF  S MV   R+ V +  +R V+++W FL  VL QSYTASL+S+L +  L P   +++
Sbjct: 627 FWFSFSIMVFAPRERVLSFWARVVVIIWYFLVLVLTQSYTASLASLLTTQHLHPTVTNIN 686

Query: 608 ELISKGYYVGYQKGSFLKSMLIEQLKFNESKLKSYANVEEYRKALSKGSQNGGVAAIFDE 667
            L++KG  VGYQ  SF+   L +   F+E+ L SY + E     LSKG   GGV+A+  E
Sbjct: 687 SLLAKGESVGYQ-SSFILGRLRDS-GFSEASLVSYGSPEHCDALLSKGQAEGGVSAVLME 746

Query: 668 IPYLQVFLTKYGSDFTMAGPKYRTDGFGFAFPLNSRLVPYVSRAILNVTESEKMVAIQTK 727
           +PY+++FL +Y + + M    ++ DG GF FP+ S LV  +SRAIL V ES K   ++  
Sbjct: 747 VPYVRIFLGQYCNKYKMVQTPFKVDGLGFVFPIGSPLVADISRAILKVEESNKANQLENA 806

Query: 728 YFGAGNQNEDSSISSPN-SPC-----LEASSFGGLFIITGISLLLALIGSKTFIWQ 773
           +F   +++    +++P+ +P      L   SF  LF++  I   +AL+    F++Q
Sbjct: 807 WFKPIDESCPDPLTNPDPNPSVSFRQLGFDSFWVLFLVAAIVCTMALL---KFVYQ 845

BLAST of Cp4.1LG04g12860 vs. TAIR10
Match: AT2G24710.1 (AT2G24710.1 glutamate receptor 2.3)

HSP 1 Score: 575.1 bits (1481), Expect = 7.1e-164
Identity = 319/776 (41.11%), Postives = 470/776 (60.57%), Query Frame = 1

Query: 7   VAVVDLVKNQKVHAIIGPESSSEATFMIKLGEKVRVPIISFSATSLSISPSHSPFFVRTA 66
           +A +DL+KN++V AI+GP +S +A F+I++G+K RVPI+S+SATS  ++   SP+F+R  
Sbjct: 85  IAALDLIKNKQVKAILGPWTSMQAHFLIEIGQKSRVPIVSYSATSPILTSLRSPYFLRAT 144

Query: 67  QNDSSQVKAITAIVQGFGWHELVLIYEDTEYGKGLIPFLTDELQQSNIRVPYKYAIATSM 126
             DS QV+ I AI++ FGW E+V +Y D  +G+G++P LTD LQ  N+R+PY+  IA + 
Sbjct: 145 YEDSFQVQPIKAIIKLFGWREVVPVYIDNTFGEGIMPRLTDALQDINVRIPYRSVIAINA 204

Query: 127 DAYQISEQLNMMKNRQTRVFLVHVTSPFGSVLFPLVDKAGMMSEGYAWILTNSLSNCLDA 186
             ++IS +L  M N  TRVFLVH+     S  F    + G+M  GY WILTN + + L  
Sbjct: 205 TDHEISVELLKMMNMPTRVFLVHMYYDLASRFFIKAKELGLMEPGYVWILTNGVIDDLSL 264

Query: 187 MDPLVIKSMEGVLGIRPYFPASEALESFKRRWKWSTP--ELNIYGLWAYDTIWALATAAE 246
           ++   +++MEGVLGI+ Y P S  LE F+ RW+   P  EL++YGLWAYD   ALA A E
Sbjct: 265 INETAVEAMEGVLGIKTYIPKSPDLEKFRSRWRSLFPRVELSVYGLWAYDATTALAVAIE 324

Query: 247 RIGEGNNLRFLR--GQGSDVEGKTDIANLGVSEVGPKLLKEMLNIKFQGLSGNFHLVNGH 306
             G  NN+ F +    G +V   +++  LG+S+ GPKLL+ +L ++F+GL+G F    G 
Sbjct: 325 EAGT-NNMTFSKVVDTGRNV---SELEALGLSQFGPKLLQTLLTVQFRGLAGEFRFFRGQ 384

Query: 307 LQPSAFEIFNLIGRGERLIGCWSPEKGICRNI-SDTKPTEKYSTSVSKLKKIIWPGDSIT 366
           LQPS FEI N+I  GE+ IG W    G+ + +          ST    LK I+WPG++ +
Sbjct: 385 LQPSVFEIVNIINTGEKSIGFWKEGNGLVKKLDQQASSISALSTWKDHLKHIVWPGEADS 444

Query: 367 APKGWAVPANGEKIRIGVPKKQGFNEFLDVTRNPLTGELNFSGFCIDVFRAVADALPFPF 426
            PKGW +P  G+K+RIGVPK+ G+ + + VTR+P+T     +GFCID F AV   LP+  
Sbjct: 445 VPKGWQIPTKGKKLRIGVPKRTGYTDLVKVTRDPITNSTVVTGFCIDFFEAVIRELPYDV 504

Query: 427 PYEFELSRDEAGDSSVIYDDLLHQLNENEKKFDVVVGDITIVASRANYVDFSLPFTDSGV 486
            YEF       G ++  Y+DL++Q+     ++D VVGD TI+ +R++YVDF+ PF  SGV
Sbjct: 505 SYEFIPFEKPDGKTAGNYNDLVYQVYLG--RYDAVVGDTTILVNRSSYVDFTFPFIKSGV 564

Query: 487 TMLVPVKRNLHRSMWVFLKPLSLGLWLTTIAVSIATGAVLLILEHNGRSE-SLHPLNLLC 546
            ++V +   + R   +F+KPLS  LWLT+       G  + +LE+    + S  P     
Sbjct: 565 GLIVEMTDPVKRDYILFMKPLSWKLWLTSFISFFLVGCTVWVLEYKRNPDFSGPPRFQAS 624

Query: 547 LILWFPVSSMVLPERQIVTNTRSRFVLVVWLFLAFVLMQSYTASLSSILMSDQLQPKYFS 606
            I WF  S+MV   R+ V +  +R +++ W FL  VL QSYTASL+S+L S +L P   S
Sbjct: 625 TICWFAFSTMVFAPRERVFSFWARALVIAWYFLVLVLTQSYTASLASLLTSQKLNPTITS 684

Query: 607 VSELISKGYYVGYQKGSFLKSMLIEQLKFNESKLKSYANVEEYRKALSKGSQNGGVAAIF 666
           +S L+ KG  VGYQ+ SF+   L E+  F +S L  +   EE  + LSKG + GGV+  F
Sbjct: 685 MSSLLEKGETVGYQRTSFILGKLKER-GFPQSSLVPFDTAEECDELLSKGPKKGGVSGAF 744

Query: 667 DEIPYLQVFLTKYGSDFTMAGPKYRTDGFGFAFPLNSRLVPYVSRAILNVTESEKMVAIQ 726
            EIPYL++FL ++ + + M    +  DGFGF FP+ S LV  VSRAIL V ES K + ++
Sbjct: 745 LEIPYLRLFLGQFCNTYKMVEEPFNVDGFGFVFPIGSPLVADVSRAILKVAESPKAMELE 804

Query: 727 TKYFGAGNQNEDSSISSPN------SPCLEASSFGGLFIITGISLLLAL-IGSKTF 770
             +F    Q+    I++P+      S  L+  SF  LF+  G+ L+  + +G+ T+
Sbjct: 805 RAWFKKKEQSCPDPITNPDPNPSFTSRQLDIDSF--LFLFVGVLLVCVMALGNFTY 851

BLAST of Cp4.1LG04g12860 vs. TAIR10
Match: AT2G29110.1 (AT2G29110.1 glutamate receptor 2.8)

HSP 1 Score: 573.2 bits (1476), Expect = 2.7e-163
Identity = 330/841 (39.24%), Postives = 488/841 (58.03%), Query Frame = 1

Query: 3   IRLMVAVVDLVKNQKVHAIIGPESSSEATFMIKLGEKVRVPIISFSATSLSISPSHSPFF 62
           ++   A +DL++N++V AIIGP  S +A FMIKL  K +VP ISFSATS  ++   S +F
Sbjct: 83  VQASAAALDLIQNEQVSAIIGPIDSMQAKFMIKLANKTQVPTISFSATSPLLTSIKSDYF 142

Query: 63  VRTAQNDSSQVKAITAIVQGFGWHELVLIYEDTEYGKGLIPFLTDELQQSNIRVPYKYAI 122
           VR   +DS QVKAI AI + FGW  +V IY D E G+G++P+L D LQ   +    +  I
Sbjct: 143 VRGTIDDSYQVKAIAAIFESFGWRSVVAIYVDNELGEGIMPYLFDALQDVQVD---RSVI 202

Query: 123 ATSMDAYQISEQLNMMKNRQTRVFLVHVTSPFGSVLFPLVDKAGMMSEGYAWILTNSLSN 182
            +  +  QI ++L  +  RQTRVF+VH+ S   S +F    + GMM EGY W++TN +++
Sbjct: 203 PSEANDDQILKELYKLMTRQTRVFVVHMASRLASRIFEKATEIGMMEEGYVWLMTNGMTH 262

Query: 183 CLDAM-DPLVIKSMEGVLGIRPYFPASEALESFKRRWK--------WSTPELNIYGLWAY 242
            +  +     + +++GVLG+R + P S+ LE F+ RWK        W   +L+I+GLWAY
Sbjct: 263 MMRHIHHGRSLNTIDGVLGVRSHVPKSKGLEDFRLRWKRNFKKENPWLRDDLSIFGLWAY 322

Query: 243 DTIWALATAAERIGEGNNLRFLRGQGSDVEGKTDIANLGVSEVGPKLLKEMLNIKFQGLS 302
           D+  ALA A E+    ++  +    GS     TD+  L VS  GP LL+ +  I+F GL+
Sbjct: 323 DSTTALAMAVEKTNI-SSFPYNNASGSS-NNMTDLGTLHVSRYGPSLLEALSEIRFNGLA 382

Query: 303 GNFHLVNGHLQPSAFEIFNLIGRGERLIGCWSPEKGICRNISDTKPTEKYSTSVSKLKKI 362
           G F+L++  L+   FEI N +G  ER++G W+P  G+  N++  K T   S +  +   +
Sbjct: 383 GRFNLIDRQLESPKFEIINFVGNEERIVGFWTPSNGLV-NVNSNKTT---SFTGERFGPL 442

Query: 363 IWPGDSITAPKGWAVPANGEKIRIGVPKKQGFNEFLDVTRNPLTGELNFSGFCIDVFRAV 422
           IWPG S   PKGW +P NG+KI++GVP K+GF  F++V  +P+T      G+ ID+F A 
Sbjct: 443 IWPGKSTIVPKGWEIPTNGKKIKVGVPVKKGFFNFVEVITDPITNITTPKGYAIDIFEAA 502

Query: 423 ADALPF---PFPYEFELSRDEAGDSSVIYDDLLHQLNENEKKFDVVVGDITIVASRANYV 482
              LP+   P  Y FE   D+       YDDL+++++      D VVGD+TI A R+ Y 
Sbjct: 503 LKKLPYSVIPQYYRFESPDDD-------YDDLVYKVDNG--TLDAVVGDVTITAYRSLYA 562

Query: 483 DFSLPFTDSGVTMLVPVKRNLHRSMWVFLKPLSLGLWLTTIAVSIATGAVLLILEHNGRS 542
           DF+LP+T+SGV+M+VPV+ N +++ WVFLKP  L LW+TT    +  G V+ + EH   +
Sbjct: 563 DFTLPYTESGVSMMVPVRDNENKNTWVFLKPWGLDLWVTTACFFVLIGFVVWLFEHRVNT 622

Query: 543 ESL-HPLNLLCLILWFPVSSMVLPERQIVTNTRSRFVLVVWLFLAFVLMQSYTASLSSIL 602
           +    P + +    WF  S+MV   R+ V +  +RFV+VVW F+  VL QSYTA+L+S L
Sbjct: 623 DFRGPPHHQIGTSFWFSFSTMVFAHREKVVSNLARFVVVVWCFVVLVLTQSYTANLTSFL 682

Query: 603 MSDQLQPKYFSVSELISKGYYVGYQKGSFLKSMLIEQLKFNESKLKSYANVEEYRKALSK 662
              + QP   +V +LI  G YVGYQ G+F+K  LI++  FN SKLK + + EE    LS 
Sbjct: 683 TVQRFQPAAINVKDLIKNGDYVGYQHGAFVKDFLIKE-GFNVSKLKPFGSSEECHALLS- 742

Query: 663 GSQNGGVAAIFDEIPYLQVFLTKYGSDFTMAGPKYRTDGFGFAFPLNSRLVPYVSRAILN 722
              NG ++A FDE+ YL+  L++Y S + +  P ++T GFGFAFP NS L   VS+AILN
Sbjct: 743 ---NGSISAAFDEVAYLRAILSQYCSKYAIVEPTFKTAGFGFAFPRNSPLTGDVSKAILN 802

Query: 723 VTESEKMVAIQTKYFGAGNQNEDSSISSPNSPCLEASSFGGLFIITGISLLLALI----- 782
           VT+ ++M  I+ K+F   N   D   ++ +S  L   SF GLF+I GI+  LAL+     
Sbjct: 803 VTQGDEMQHIENKWFMKQNDCPDPK-TALSSNRLSLRSFWGLFLIAGIASFLALLIFVFL 862

Query: 783 -----------GSKTFIWQKPASVAKTYYRKYVSFQQHLHSDVKDKVMDDRLKLPNTNTL 815
                       S+  IW+K  S+ + +  K +       S V          +P+ +TL
Sbjct: 863 FLYENRHTLCDDSEDSIWRKLTSLFRNFDEKDIKSHTFKSSAVHHVSSPMTQYIPSPSTL 899

BLAST of Cp4.1LG04g12860 vs. TAIR10
Match: AT2G29120.1 (AT2G29120.1 glutamate receptor 2.7)

HSP 1 Score: 572.4 bits (1474), Expect = 4.6e-163
Identity = 324/771 (42.02%), Postives = 474/771 (61.48%), Query Frame = 1

Query: 8   AVVDLVKNQKVHAIIGPESSSEATFMIKLGEKVRVPIISFSATSLSISPSHSPFFVRTAQ 67
           A +DL+KN++V AIIGP +S +A FMI+L +K +VP I+FSAT   ++  +SP+FVR   
Sbjct: 94  AALDLIKNEQVSAIIGPRTSMQAEFMIRLADKSQVPTITFSATCPLLTSINSPYFVRATL 153

Query: 68  NDSSQVKAITAIVQGFGWHELVLIYEDTEYGKGLIPFLTDELQQSNIRVPYKYAIATSMD 127
           +DSSQVKAI AIV+ FGW  +V IY D E+G+G++P LTD LQ     V  +  I    +
Sbjct: 154 DDSSQVKAIAAIVKSFGWRNVVAIYVDNEFGEGILPLLTDALQDVQAFVVNRCLIPQEAN 213

Query: 128 AYQISEQLNMMKNRQTRVFLVHVTSPFGSVLFPLVDKAGMMSEGYAWILTNSLSNCLDAM 187
             QI ++L  +   QTRVF+VH+    G   F    + GMM EGY W+LT+ + N L + 
Sbjct: 214 DDQILKELYKLMTMQTRVFVVHMPPTLGFRFFQKAREIGMMEEGYVWLLTDGVMNLLKSN 273

Query: 188 D-PLVIKSMEGVLGIRPYFPASEALESFKRRW------KWSTPELNIYGLWAYDTIWALA 247
           +    +++M+GVLG+R + P S+ L++F+ RW      K +  E+NI+ L AYD+I ALA
Sbjct: 274 ERGSSLENMQGVLGVRSHIPKSKKLKNFRLRWEKMFPKKGNDEEMNIFALRAYDSITALA 333

Query: 248 TAAERIGEGNNLRFLRGQGSDVEG--KTDIANLGVSEVGPKLLKEMLNIKFQGLSGNFHL 307
            A E+     N++ LR       G  KT++  LGVS  GP LLK + N++F GL+G F L
Sbjct: 334 MAVEK----TNIKSLRYDHPIASGNNKTNLGTLGVSRYGPSLLKALSNVRFNGLAGEFEL 393

Query: 308 VNGHLQPSAFEIFNLIGRGERLIGCWSPEKGICRNISDTKPTEKYSTSV--SKLKKIIWP 367
           +NG L+ S F++ N+IG  ER+IG W P  GI           K +TSV   +L  +IWP
Sbjct: 394 INGQLESSVFDVINIIGSEERIIGLWRPSNGI------VNAKSKNTTSVLGERLGPVIWP 453

Query: 368 GDSITAPKGWAVPANGEKIRIGVPKKQGFNEFLDVTRNPLTGELNFSGFCIDVFRAVADA 427
           G S   PKGW +P NG+ +R+G+P K+GF EF+D   +P++  +  +G+CI++F AV   
Sbjct: 454 GKSKDVPKGWQIPTNGKMLRVGIPVKKGFLEFVDAKIDPISNAMTPTGYCIEIFEAVLKK 513

Query: 428 LPFPF--PYEFELSRDEAGDSSVIYDDLLHQLNENEKKFDVVVGDITIVASRANYVDFSL 487
           LP+     Y   LS DE       YD++++Q+      +D VVGD+TIVA+R+ YVDF+L
Sbjct: 514 LPYSVIPKYIAFLSPDEN------YDEMVYQVYTG--AYDAVVGDVTIVANRSLYVDFTL 573

Query: 488 PFTDSGVTMLVPVKRNLHRSMWVFLKPLSLGLWLTTIAVSIATGAVLLILEHNGRSESL- 547
           P+T+SGV+M+VP+K N  ++ WVFL+P SL LW+TT    +  G ++ ILEH   ++   
Sbjct: 574 PYTESGVSMMVPLKDN--KNTWVFLRPWSLDLWVTTACFFVFIGFIVWILEHRVNTDFRG 633

Query: 548 HPLNLLCLILWFPVSSMVLPERQIVTNTRSRFVLVVWLFLAFVLMQSYTASLSSILMSDQ 607
            P + +    WF  S+M    R+ V +  +RFV++VW F+  VL+QSYTA+L+S      
Sbjct: 634 PPHHQIGTSFWFAFSTMNFAHREKVVSNLARFVVLVWCFVVLVLIQSYTANLTSFFTVKL 693

Query: 608 LQPKYFSVSELISKGYYVGYQKGSFLKSMLIEQLKFNESKLKSYANVEEYRKALSKGSQN 667
           LQP   +  +LI     +GYQ+G+F++ +L  Q  F+ES+LK + +  E  +  S    N
Sbjct: 694 LQPTVTNWKDLIKFNKNIGYQRGTFVRELLKSQ-GFDESQLKPFGSAVECDELFS----N 753

Query: 668 GGVAAIFDEIPYLQVFLTKYGSDFTMAGPKYRTDGFGFAFPLNSRLVPYVSRAILNVTES 727
           G + A FDE+ Y++V L++  S +TM  P ++T GFGF FP  S L   VSRAILNVT+ 
Sbjct: 754 GTITASFDEVAYIKVILSQNSSKYTMVEPSFKTAGFGFVFPKKSPLTDDVSRAILNVTQG 813

Query: 728 EKMVAIQTKYFGAGNQNEDSSISSPNSPCLEASSFGGLFIITGISLLLALI 765
           E+M  I+ K+F   N   D + +S +S  L  SSF GLF+I GI+  LAL+
Sbjct: 814 EEMQHIENKWFKKPNNCPDLN-TSLSSNHLSLSSFWGLFLIAGIASFLALL 838

BLAST of Cp4.1LG04g12860 vs. NCBI nr
Match: gi|659107394|ref|XP_008453652.1| (PREDICTED: glutamate receptor 2.2-like [Cucumis melo])

HSP 1 Score: 1419.4 bits (3673), Expect = 0.0e+00
Identity = 709/831 (85.32%), Postives = 758/831 (91.22%), Query Frame = 1

Query: 8   AVVDLVKNQKVHAIIGPESSSEATFMIKLGEKVRVPIISFSATSLSISPSHSPFFVRTAQ 67
           A VDLVKN+KVHAIIGPESS EATFMIKLG+K RVPIISFSATSLSISPSHSPFFVRTAQ
Sbjct: 107 AAVDLVKNEKVHAIIGPESSGEATFMIKLGDKARVPIISFSATSLSISPSHSPFFVRTAQ 166

Query: 68  NDSSQVKAITAIVQGFGWHELVLIYEDTEYGKGLIPFLTDELQQSNIRVPYKYAIATSMD 127
           NDSSQVKAIT +VQGFGWH+LVLIYEDTEYG+GLIPFLTD LQ+SNIRV +KYAI  SMD
Sbjct: 167 NDSSQVKAITTLVQGFGWHDLVLIYEDTEYGRGLIPFLTDALQESNIRVSFKYAIPASMD 226

Query: 128 AYQISEQLNMMKNRQTRVFLVHVTSPFGSVLFPLVDKAGMMSEGYAWILTNSLSNCLDAM 187
            Y+IS+ L+ MK RQTRVFLVHVTSPFGS LFPLV+KAGMM+EGYAW+LTN+LSNCLDAM
Sbjct: 227 PYEISKHLHNMKKRQTRVFLVHVTSPFGSALFPLVEKAGMMTEGYAWLLTNTLSNCLDAM 286

Query: 188 DPLVIKSMEGVLGIRPYFPASEALESFKRRWKWSTPELNIYGLWAYDTIWALATAAERIG 247
           DPLVIKSMEGVLGIRP+FPASE LE+FKRRWKWS PELNIYGLWAYDTIWALA AAERIG
Sbjct: 287 DPLVIKSMEGVLGIRPHFPASEDLENFKRRWKWSAPELNIYGLWAYDTIWALAMAAERIG 346

Query: 248 EGNNLRFLRGQGSDVEGKTDIANLGVSEVGPKLLKEMLNIKFQGLSGNFHLVNGHLQPSA 307
           + +NL FL+G+ SDVEGKTDIANLGVSEVGP LLKEMLNIKF+GLSG+FHLVNGHLQPSA
Sbjct: 347 DVSNLGFLKGRVSDVEGKTDIANLGVSEVGPMLLKEMLNIKFKGLSGDFHLVNGHLQPSA 406

Query: 308 FEIFNLIGRGERLIGCWSPEKGICRNISDTKPTEKYSTSVSKLKKIIWPGDSITAPKGWA 367
           FEIFN+IG  ERLIGCW+PE+GIC+NI++ KP EKYSTSVSKLKKIIWPGDSITAPKGWA
Sbjct: 407 FEIFNVIGTAERLIGCWNPEEGICQNIANKKPNEKYSTSVSKLKKIIWPGDSITAPKGWA 466

Query: 368 VPANGEKIRIGVPKKQGFNEFLDVTRNPLTGELNFSGFCIDVFRAVADALPFPFPYEFEL 427
           VPA+GEK RIGVPKKQGFNEFLDVTRNP  GELNF+GFCIDVFRAVADALPFP PYEFEL
Sbjct: 467 VPADGEKFRIGVPKKQGFNEFLDVTRNPQNGELNFTGFCIDVFRAVADALPFPLPYEFEL 526

Query: 428 SRDEAGDSSVIYDDLLHQLNENEK-KFDVVVGDITIVASRANYVDFSLPFTDSGVTMLVP 487
             D+AGD+SVIYDDLLHQL E EK KFD VVGDITIVASRAN VDFSLP+TDSGVTMLVP
Sbjct: 527 FEDDAGDNSVIYDDLLHQLAEREKNKFDAVVGDITIVASRANLVDFSLPYTDSGVTMLVP 586

Query: 488 VKRNLHRSMWVFLKPLSLGLWLTTIAVSIATGAVLLILEHNGRSESLHPLNLLCLILWFP 547
           +K N+HRSMWVFLKPLSL LWLTTIA SIATG VLLILE N R ESL PL LLCLILWFP
Sbjct: 587 IKHNMHRSMWVFLKPLSLDLWLTTIAASIATGIVLLILEQNARRESLQPLELLCLILWFP 646

Query: 548 VSSMVLPERQIVTNTRSRFVLVVWLFLAFVLMQSYTASLSSILMSDQLQPKYFSVSELIS 607
            SS+VLPERQIVTNTRSRFVLVVWLFLAFVLMQSYTASLSSILMSDQLQPKYFSV+ELIS
Sbjct: 647 FSSLVLPERQIVTNTRSRFVLVVWLFLAFVLMQSYTASLSSILMSDQLQPKYFSVNELIS 706

Query: 608 KGYYVGYQKGSFLKSMLIEQLKFNESKLKSYANVEEYRKALSKGSQNGGVAAIFDEIPYL 667
           KGYYVGYQ+GSF KSMLIEQLKFNESKLKSYANVEE+ KALSKGSQNGGVAAIFDEIPYL
Sbjct: 707 KGYYVGYQEGSFTKSMLIEQLKFNESKLKSYANVEEFHKALSKGSQNGGVAAIFDEIPYL 766

Query: 668 QVFLTKYGSDFTMAGPKYRTDGFGFAFPLNSRLVPYVSRAILNVTESEKMVAIQTKYFGA 727
           +VFLTKYGSDF MAGPKYRTDGFGFAFPLNSRLVPYVSRAILNVTE EKMV I+TKYFGA
Sbjct: 767 KVFLTKYGSDFIMAGPKYRTDGFGFAFPLNSRLVPYVSRAILNVTEGEKMVTIETKYFGA 826

Query: 728 GNQNEDSSISSPNSPCLEASSFGGLFIITGISLLLALIGSKTFIWQKPASVAKTYYRKYV 787
           GNQN+DSS SS + PCLE SSFGGLFIITGI+ LLALI S+TFIWQKPASVAKTYYRKYV
Sbjct: 827 GNQNQDSSNSSSDGPCLEVSSFGGLFIITGIAFLLALIDSQTFIWQKPASVAKTYYRKYV 886

Query: 788 SFQQHLHSDVKDKVMDDRLKLPNTNTLETVSAGADHGCHDGCSSPAKHVTE 838
           SF++  HSDVKDK MDD  K         VSA ADHGCHDG + P+KHVTE
Sbjct: 887 SFKEDSHSDVKDKEMDDMSK------SSEVSADADHGCHDGSAGPSKHVTE 931

BLAST of Cp4.1LG04g12860 vs. NCBI nr
Match: gi|449457227|ref|XP_004146350.1| (PREDICTED: glutamate receptor 2.8-like [Cucumis sativus])

HSP 1 Score: 1408.7 bits (3645), Expect = 0.0e+00
Identity = 705/831 (84.84%), Postives = 758/831 (91.22%), Query Frame = 1

Query: 8   AVVDLVKNQKVHAIIGPESSSEATFMIKLGEKVRVPIISFSATSLSISPSHSPFFVRTAQ 67
           AVVDLVKN+KVHAIIGPESS EATFMIKLGEK  VPI+SFSATSLSISPSHSPFFVRTAQ
Sbjct: 33  AVVDLVKNEKVHAIIGPESSGEATFMIKLGEKAHVPIVSFSATSLSISPSHSPFFVRTAQ 92

Query: 68  NDSSQVKAITAIVQGFGWHELVLIYEDTEYGKGLIPFLTDELQQSNIRVPYKYAIATSMD 127
           NDSSQV+AIT IVQGFGWH+LVLIYEDTEYG+GLIPFLTD LQ+SNIRV +KYAI TSMD
Sbjct: 93  NDSSQVQAITTIVQGFGWHDLVLIYEDTEYGRGLIPFLTDALQESNIRVSFKYAIPTSMD 152

Query: 128 AYQISEQLNMMKNRQTRVFLVHVTSPFGSVLFPLVDKAGMMSEGYAWILTNSLSNCLDAM 187
            Y+IS+ L+ MK RQTRVFLVHVTSPFGS LFPLV+KAGMM+EGYAW+LTN+LSNCLDAM
Sbjct: 153 PYEISQHLHKMKKRQTRVFLVHVTSPFGSALFPLVEKAGMMTEGYAWLLTNTLSNCLDAM 212

Query: 188 DPLVIKSMEGVLGIRPYFPASEALESFKRRWKWSTPELNIYGLWAYDTIWALATAAERIG 247
           DP VIKSMEGVLGIRP+FPASEALE+FKRRWKWS PELNIYGLWAYDTIWALA AAERIG
Sbjct: 213 DPSVIKSMEGVLGIRPHFPASEALENFKRRWKWSAPELNIYGLWAYDTIWALAMAAERIG 272

Query: 248 EGNNLRFLRGQGSDVEGKTDIANLGVSEVGPKLLKEMLNIKFQGLSGNFHLVNGHLQPSA 307
           + +NL FL+G+GSDVEGKTDIANL VSEVGP LLKEMLNIKF+GLSG+FHLVNGHLQPSA
Sbjct: 273 DVSNLGFLKGRGSDVEGKTDIANLRVSEVGPMLLKEMLNIKFKGLSGDFHLVNGHLQPSA 332

Query: 308 FEIFNLIGRGERLIGCWSPEKGICRNISDTKPTEKYSTSVSKLKKIIWPGDSITAPKGWA 367
           FEIFN+IGR ERLIG W+PE+GIC+NI++ KP EKYSTSVSKLKKIIWPGDSITAP+GWA
Sbjct: 333 FEIFNMIGRAERLIGYWNPEEGICQNIANKKPNEKYSTSVSKLKKIIWPGDSITAPRGWA 392

Query: 368 VPANGEKIRIGVPKKQGFNEFLDVTRNPLTGELNFSGFCIDVFRAVADALPFPFPYEFEL 427
           VPA+GEK RIGVPKKQGFNEFLDVTRNP TGELNF+GFCIDVFRAVADALPFP PYEFEL
Sbjct: 393 VPADGEKFRIGVPKKQGFNEFLDVTRNPQTGELNFTGFCIDVFRAVADALPFPLPYEFEL 452

Query: 428 SRDEAGDSSVIYDDLLHQLNENEK-KFDVVVGDITIVASRANYVDFSLPFTDSGVTMLVP 487
            +D+AGD+SVIYDDLLHQL E EK KFD VVGDITIVASRAN VDFSLP+TDSGVTMLVP
Sbjct: 453 FKDDAGDNSVIYDDLLHQLAEREKNKFDAVVGDITIVASRANLVDFSLPYTDSGVTMLVP 512

Query: 488 VKRNLHRSMWVFLKPLSLGLWLTTIAVSIATGAVLLILEHNGRSESLHPLNLLCLILWFP 547
           +K N+HRSMWVFLKPLSL LWLTTIA SIATG VLLILE N R ESL PL LLCLILWFP
Sbjct: 513 IKHNMHRSMWVFLKPLSLDLWLTTIAASIATGIVLLILEQNARRESLQPLELLCLILWFP 572

Query: 548 VSSMVLPERQIVTNTRSRFVLVVWLFLAFVLMQSYTASLSSILMSDQLQPKYFSVSELIS 607
            SS+VLPERQIVTNTRSRFVLVVWLFLAFVLMQSYTASLSSILMSDQLQPKYFSV+ELIS
Sbjct: 573 FSSLVLPERQIVTNTRSRFVLVVWLFLAFVLMQSYTASLSSILMSDQLQPKYFSVNELIS 632

Query: 608 KGYYVGYQKGSFLKSMLIEQLKFNESKLKSYANVEEYRKALSKGSQNGGVAAIFDEIPYL 667
           KGYYVGYQ+GSF KSMLIEQLKFNESKLKSYANVEE+ KALSKGSQNGGVAAIFDEIPYL
Sbjct: 633 KGYYVGYQEGSFTKSMLIEQLKFNESKLKSYANVEEFHKALSKGSQNGGVAAIFDEIPYL 692

Query: 668 QVFLTKYGSDFTMAGPKYRTDGFGFAFPLNSRLVPYVSRAILNVTESEKMVAIQTKYFGA 727
           +VFLTKYGSDF  AGP YRTDGFGFAFPLNSRLVPYVSRAILNVTE EKMVAI+TKYFGA
Sbjct: 693 KVFLTKYGSDFIRAGPIYRTDGFGFAFPLNSRLVPYVSRAILNVTEGEKMVAIETKYFGA 752

Query: 728 GNQNEDSSISSPNSPCLEASSFGGLFIITGISLLLALIGSKTFIWQKPASVAKTYYRKYV 787
           GNQN+DSS SS + PCLE SSFGGLFIITGI+ LLALI S+TFIW+KPASVAKTYYRKYV
Sbjct: 753 GNQNQDSSNSSSDGPCLEVSSFGGLFIITGIAFLLALIDSQTFIWRKPASVAKTYYRKYV 812

Query: 788 SFQQHLHSDVKDKVMDDRLKLPNTNTLETVSAGADHGCHDGCSSPAKHVTE 838
           SF++  HSDVKD+ MDD  K         VSA  DHGC DG + P+KHVTE
Sbjct: 813 SFKEDSHSDVKDEEMDDISK------SSEVSADVDHGCLDGSAGPSKHVTE 857

BLAST of Cp4.1LG04g12860 vs. NCBI nr
Match: gi|700210316|gb|KGN65412.1| (hypothetical protein Csa_1G418780 [Cucumis sativus])

HSP 1 Score: 959.5 bits (2479), Expect = 3.8e-276
Identity = 468/548 (85.40%), Postives = 506/548 (92.34%), Query Frame = 1

Query: 8   AVVDLVKNQKVHAIIGPESSSEATFMIKLGEKVRVPIISFSATSLSISPSHSPFFVRTAQ 67
           AVVDLVKN+KVHAIIGPESS EATFMIKLGEK  VPI+SFSATSLSISPSHSPFFVRTAQ
Sbjct: 7   AVVDLVKNEKVHAIIGPESSGEATFMIKLGEKAHVPIVSFSATSLSISPSHSPFFVRTAQ 66

Query: 68  NDSSQVKAITAIVQGFGWHELVLIYEDTEYGKGLIPFLTDELQQSNIRVPYKYAIATSMD 127
           NDSSQV+AIT IVQGFGWH+LVLIYEDTEYG+GLIPFLTD LQ+SNIRV +KYAI TSMD
Sbjct: 67  NDSSQVQAITTIVQGFGWHDLVLIYEDTEYGRGLIPFLTDALQESNIRVSFKYAIPTSMD 126

Query: 128 AYQISEQLNMMKNRQTRVFLVHVTSPFGSVLFPLVDKAGMMSEGYAWILTNSLSNCLDAM 187
            Y+IS+ L+ MK RQTRVFLVHVTSPFGS LFPLV+KAGMM+EGYAW+LTN+LSNCLDAM
Sbjct: 127 PYEISQHLHKMKKRQTRVFLVHVTSPFGSALFPLVEKAGMMTEGYAWLLTNTLSNCLDAM 186

Query: 188 DPLVIKSMEGVLGIRPYFPASEALESFKRRWKWSTPELNIYGLWAYDTIWALATAAERIG 247
           DP VIKSMEGVLGIRP+FPASEALE+FKRRWKWS PELNIYGLWAYDTIWALA AAERIG
Sbjct: 187 DPSVIKSMEGVLGIRPHFPASEALENFKRRWKWSAPELNIYGLWAYDTIWALAMAAERIG 246

Query: 248 EGNNLRFLRGQGSDVEGKTDIANLGVSEVGPKLLKEMLNIKFQGLSGNFHLVNGHLQPSA 307
           + +NL FL+G+GSDVEGKTDIANL VSEVGP LLKEMLNIKF+GLSG+FHLVNGHLQPSA
Sbjct: 247 DVSNLGFLKGRGSDVEGKTDIANLRVSEVGPMLLKEMLNIKFKGLSGDFHLVNGHLQPSA 306

Query: 308 FEIFNLIGRGERLIGCWSPEKGICRNISDTKPTEKYSTSVSKLKKIIWPGDSITAPKGWA 367
           FEIFN+IGR ERLIG W+PE+GIC+NI++ KP EKYSTSVSKLKKIIWPGDSITAP+GWA
Sbjct: 307 FEIFNMIGRAERLIGYWNPEEGICQNIANKKPNEKYSTSVSKLKKIIWPGDSITAPRGWA 366

Query: 368 VPANGEKIRIGVPKKQGFNEFLDVTRNPLTGELNFSGFCIDVFRAVADALPFPFPYEFEL 427
           VPA+GEK RIGVPKKQGFNEFLDVTRNP TGELNF+GFCIDVFRAVADALPFP PYEFEL
Sbjct: 367 VPADGEKFRIGVPKKQGFNEFLDVTRNPQTGELNFTGFCIDVFRAVADALPFPLPYEFEL 426

Query: 428 SRDEAGDSSVIYDDLLHQLNENEK-KFDVVVGDITIVASRANYVDFSLPFTDSGVTMLVP 487
            +D+AGD+SVIYDDLLHQL E EK KFD VVGDITIVASRAN VDFSLP+TDSGVTMLVP
Sbjct: 427 FKDDAGDNSVIYDDLLHQLAEREKNKFDAVVGDITIVASRANLVDFSLPYTDSGVTMLVP 486

Query: 488 VKRNLHRSMWVFLKPLSLGLWLTTIAVSIATGAVLLILEHNGRSESLHPLNLLCLILWFP 547
           +K N+HRSMWVFLKPLSL LWLTTIA SIATG VLLILE N R ESL PL LLCLILWFP
Sbjct: 487 IKHNMHRSMWVFLKPLSLDLWLTTIAASIATGIVLLILEQNARRESLQPLELLCLILWFP 546

Query: 548 VSSMVLPE 555
            SS+VLPE
Sbjct: 547 FSSLVLPE 554

BLAST of Cp4.1LG04g12860 vs. NCBI nr
Match: gi|590693027|ref|XP_007044219.1| (Glutamate receptor 2.8, putative [Theobroma cacao])

HSP 1 Score: 782.7 bits (2020), Expect = 6.4e-223
Identity = 410/818 (50.12%), Postives = 566/818 (69.19%), Query Frame = 1

Query: 8   AVVDLVKNQKVHAIIGPESSSEATFMIKLGEKVRVPIISFSATSLSISPSHSPFFVRTAQ 67
           +VVDL+KN++ HAIIGP+ S+EA F I+LGE   VP++SFSATS S+SP+ + +F+RTAQ
Sbjct: 80  SVVDLLKNEQAHAIIGPQWSAEAKFAIELGEMAHVPMVSFSATSPSLSPTQNTYFIRTAQ 139

Query: 68  NDSSQVKAITAIVQGFGWHELVLIYEDTEYGKGLIPFLTDELQQSNIRVPYKYAIATSMD 127
           ND+SQ++AI A+++ FGW ++VLI+EDTEYG  ++P+L D  ++++I + YK  I++S  
Sbjct: 140 NDASQIRAIVAVLKQFGWPQVVLIHEDTEYGTEIVPYLFDSWRENDIGLAYKSKISSSAT 199

Query: 128 AYQISEQLNMMKNRQTRVFLVHVTSPFGSVLFPLVDKAGMMSEGYAWILTNSLSNCLDAM 187
            ++IS +LN ++  QT+VFLVH+TS  GS LF LVDKAGMMS GY+W++T+ +SN L+ M
Sbjct: 200 DFEISRELNKLRALQTKVFLVHMTSSLGSRLFSLVDKAGMMSIGYSWLITDGISNSLEDM 259

Query: 188 DPLVIKSMEGVLGIRPYFPASEALESFKRRWK---------WSTPELNIYGLWAYDTIWA 247
           DP VI SMEGVLG++P+ P +  +++FK+RW+          S  ELNI+G WAYDT+WA
Sbjct: 260 DPAVIHSMEGVLGVKPHVPETHVVKTFKKRWQRNLHLLKPNSSVGELNIFGFWAYDTVWA 319

Query: 248 LATAAERIGEGNNLRFLRGQGSDVEGKTDIANLGVSEVGPKLLKEMLNIKFQGLSGNFHL 307
           LATAAERI   N      G    V    D+ANL +S++GP+ L E+LN KF+GL G+FHL
Sbjct: 320 LATAAERIVPVNPTFLKVGTNGSV---MDLANLSISKIGPRFLSEILNTKFKGLGGDFHL 379

Query: 308 VNGHLQPSAFEIFNLIGRGERLIGCWSPEKGICRNISDTKPTEKYSTSVSKLKKIIWPGD 367
           V+G LQPS FEIFN+IGRG+R++G W+P++GI R +S T+       + S LK IIWPGD
Sbjct: 380 VDGQLQPSVFEIFNVIGRGQRIVGFWTPKEGISRALSSTE-----RVASSGLKNIIWPGD 439

Query: 368 SITAPKGWAVPANGEKIRIGVPKKQGFNEFLDVTRNPLTGELNFSGFCIDVFRAVADALP 427
           S   P GWA+P     +RIGVP K GF +F+ + +N   G +  +GF I+VF A    L 
Sbjct: 440 STEVPTGWAIP----MLRIGVPVKTGFTQFVKIDKNGADG-IRCTGFSIEVFEAALKKLA 499

Query: 428 FPFPYEFELSRDEAGDSSVIYDDLLHQLNENEKKFDVVVGDITIVASRANYVDFSLPFTD 487
           F   Y +    ++ G S+  Y+DLLHQ+    K  D VVGD TI+A R +YVDF+LP+T+
Sbjct: 500 FNVSYVYVPFMNDEGKSNGSYNDLLHQI--EHKIVDAVVGDTTIIAERTSYVDFTLPYTE 559

Query: 488 SGVTMLVPVKRNLHRSMWVFLKPLSLGLWLTTIAVSIATGAVLLILEHNGRSESLH---P 547
           S + M+VP+K    +S+W FL+PL   LW T I   I  G V+ I+E +  + +     P
Sbjct: 560 SRIVMVVPIKH--EKSLWSFLQPLGWDLWFTIIGSCIFFGLVIRIMERHHTANTRFGGPP 619

Query: 548 LNLLCLILWFPVSSMVLPERQIVTNTRSRFVLVVWLFLAFVLMQSYTASLSSILMSDQLQ 607
              L +I WFP SS+V P+R+++ N  S FVLV+WLFLA++LMQSYTASLSSIL  DQL 
Sbjct: 620 SRQLGMIFWFPFSSLVFPQRELLLNDYSIFVLVMWLFLAYILMQSYTASLSSILTVDQLH 679

Query: 608 PKYFSVSELISKGYYVGYQKGSFLKSMLIEQLKFNESKLKSYANVEEYRKALSKGSQNGG 667
           P +FSV  L +KGY VGYQ GSF+   L  +LKF+ESKLK+Y  +++Y KALS GS++ G
Sbjct: 680 PTFFSVQNLKTKGYNVGYQNGSFVLDFLKNRLKFDESKLKAYDTIQDYDKALSVGSEHDG 739

Query: 668 VAAIFDEIPYLQVFLTKYGSDFTMAGPKYRTDGFGFAFPLNSRLVPYVSRAILNVTESEK 727
           VAAIFDEIP++++FL KYGS++ + GP YRTDGFGFAFP  S LVPY+SRAIL V E   
Sbjct: 740 VAAIFDEIPFIRLFLDKYGSNYMITGPTYRTDGFGFAFPRGSPLVPYISRAILKVREDTI 799

Query: 728 MVAIQTKYFGAGNQNEDSSIS-SPNSPCLEASSFGGLFIITGISLLLALIGSKTFIWQKP 787
           M  I+ KYF     +  S+   S +S  L   SFGGLFIITGI+ LLAL+ S+ + W+KP
Sbjct: 800 MDNIEKKYFKHQVTSLYSAAPISADSRSLSLHSFGGLFIITGIATLLALVISEGYFWEKP 859

Query: 788 ASVAKTYYRKYVSFQQHLHSDVKDKVMDDRLKLPNTNT 813
            S+AK Y ++Y+S +    S+++ K++     + N +T
Sbjct: 860 VSMAKKYGQRYLSSRA---SNIETKLVAHSTTVMNAST 877

BLAST of Cp4.1LG04g12860 vs. NCBI nr
Match: gi|566215177|ref|XP_002324493.2| (hypothetical protein POPTR_0018s10550g [Populus trichocarpa])

HSP 1 Score: 740.3 bits (1910), Expect = 3.6e-210
Identity = 397/768 (51.69%), Postives = 525/768 (68.36%), Query Frame = 1

Query: 8   AVVDLVKNQKVHAIIGPESSSEATFMIKLGEKVRVPIISFSATSLSISPSHSPFFVRTAQ 67
           +V+DL+KN +VHAIIGP+ SS+A F+I+LG K  VPI+SFSATS ++S + S +FVRTAQ
Sbjct: 88  SVLDLMKNDQVHAIIGPQKSSQAKFVIELGGKAEVPIVSFSATSPTLSATQSKYFVRTAQ 147

Query: 68  NDSSQVKAITAIVQGFGWHELVLIYEDTEYGKGLIPFLTDELQQSNIRVPYKYAIATSMD 127
           +DSSQVKAI++IVQ +GW E+V IYEDTEYG GL+PFL D  Q+ + RVPY   I    +
Sbjct: 148 DDSSQVKAISSIVQAYGWREIVPIYEDTEYGNGLVPFLLDAFQEIDTRVPYGSRIPLYFN 207

Query: 128 AYQISEQLNMMKNRQTRVFLVHVTSPFGSVLFPLVDKAGMMSEGYAWILTNSLSNCLDAM 187
             QI  +L+ +K  +  +FLVH+++  GS LF L   AGMMSEGYAW++T  LS  LD +
Sbjct: 208 DTQIMSELHKLKEMKKSIFLVHMSASLGSRLFLLAKDAGMMSEGYAWLVTAGLSALLDPL 267

Query: 188 DPLVIKSMEGVLGIRPYFPASEALESFKRRWK----WSTP-----ELNIYGLWAYDTIWA 247
              V+ SM+GVLGI+P+ P+S+ LESFK RW+     S P     ELN++GLWAYDT+WA
Sbjct: 268 GSEVMDSMQGVLGIKPHIPSSKKLESFKSRWRKKFTISKPQSKINELNLFGLWAYDTVWA 327

Query: 248 LATAAERIGEGNNLRFLRGQGSDVEGKTDIANLGVSEVGPKLLKEMLNIKFQGLSGNFHL 307
           +A A E+ G  ++ R+++   S  E   DIA LG SE GP+LL  +L+ +FQGLSG+FHL
Sbjct: 328 IAMAVEKAGIVHS-RYVKPNTS--ESTVDIAALGKSETGPRLLSSILSTRFQGLSGDFHL 387

Query: 308 VNGHLQPSAFEIFNLIGRGERLIGCWSPEKGICRNISDTKPTEKYSTSVSKLKKIIWPGD 367
             G   PSAFEI NLIG+ ER+IG W+PE+G+ RN+  T     YSTS +KLK+ IWPGD
Sbjct: 388 AGGERVPSAFEILNLIGKAERVIGYWTPERGLSRNLY-TNGKIAYSTSKNKLKEPIWPGD 447

Query: 368 SITAPKGWAVPANGEKIRIGVPKKQGFNEFLDVTRNPLTGELNFSGFCIDVFRAVADALP 427
           +   PK         ++RIGVP K GFNEF+ V  NP   +   SGF  DVF +V +ALP
Sbjct: 448 TTQQPK---------RLRIGVPLKTGFNEFIKVEWNPEDDKPIVSGFTRDVFVSVVEALP 507

Query: 428 FPFPYEFELSRDEAGDSSVIYDDLLHQLNENEKKFDVVVGDITIVASRANYVDFSLPFTD 487
           FP PYEF    ++   S+  Y+DLL Q+    K FD  VGDITI+A+R+ YVDF+LPF++
Sbjct: 508 FPLPYEFIPFVNKNKQSAGTYNDLLDQI--KLKNFDAAVGDITIIANRSTYVDFTLPFSE 567

Query: 488 SGVTMLVPVKRNLHRSMWVFLKPLSLGLWLTTIAVSIATGAVLLILEH-NGRSESLHPLN 547
           SG+TM+V  KR+   +MW+FLKPLSL LWLTT    I TG V+ +LEH   +     P  
Sbjct: 568 SGITMVVLTKRDERENMWIFLKPLSLELWLTTGIAFILTGLVVWVLEHRENKVFRGKPAQ 627

Query: 548 LLCLILWFPVSSMVLPERQIVTNTRSRFVLVVWLFLAFVLMQSYTASLSSILMSDQLQPK 607
            L   LWF  S++    R+ V N  +RFVL++W+F+  ++ QSYTASL+SIL   +LQP 
Sbjct: 628 QLGTTLWFSFSTLFFAHREKVVNNWTRFVLIIWIFVVLIISQSYTASLASILTVKRLQPT 687

Query: 608 YFSVSELISKGYYVGYQKGSFLKSMLIEQLKFNESKLKSYANVEEYRKALSKGSQNGGVA 667
           +  V E+   GY+VG+QK SF+K  L++QL FN++ L+ Y+  EEY  ALS+G+ NGGVA
Sbjct: 688 FVDVKEIRKNGYFVGHQKDSFVKDFLVKQLNFNDTMLREYSTPEEYHDALSRGTHNGGVA 747

Query: 668 AIFDEIPYLQVFLTKYGSDFTMAGPKYRTDGFGFAFPLNSRLVPYVSRAILNVT-ESEKM 727
           AIF EIPY+++FL KY S F M GP Y+TDGFGFAFPL S LVPY+SRAILNVT + +KM
Sbjct: 748 AIFAEIPYIKLFLAKYCSKFQMVGPTYKTDGFGFAFPLGSPLVPYISRAILNVTQDKDKM 807

Query: 728 VAIQTKYFGAGNQNEDSSISSPNSPCLEASSFGGLFIITGISLLLALI 765
             I+ + FG      D +   P S  L   SFGGLFIITG++ + AL+
Sbjct: 808 DEIERRNFGGETTCSDQAAMVP-SGGLGLPSFGGLFIITGVASMSALL 839