Cp4.1LG03g08300 (gene) Cucurbita pepo (Zucchini) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cp4.1LG03g08300
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (Zucchini))
Description	Hydroxymethylglutaryl-CoA lyase
Location	Cp4.1LG03 : 2830205 .. 2835203 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TGATCTTGATCTTCATTCTTGTTTATTGCTTTAGGACGATGCAAGCTGCAAAGGTTTCTGCTAAATTGACTCGACGGGGTTTGTCTTGGCGCCATGGATATAATCCAACCATGAGAATTTCCAGCCGGTTTCTTAGTTCATCTTCGGCTTCGCATGTAGGCGTCACCTCTACTAACGGTCTCGGCATACAAGGAGAAGTTGATAATTGCTGGTATGGATGAAATGATTGTTCTTCACAAGTTCGTAATCCAGTATGTATGATCTGATTATAGGGAGAATTAATTATACCTTTCGATGCTTTCGTTTTTTTTTTTTATTAAATCGCTTATCGTTGCAATATCTTTTCGCTGAAAATGTGCGATAGAATTCTTTAAATCCTTATGGGGTGAGTCGTGGAAGATGGAATCGTGAAATTTGGGCATTCTTTTACGATTTTACGATGGGAAATCTACTTCGACGAATTTGGGGGCATCCGTTCAAGAGTAGAGCTAATAAGCTTTGGGTAAATGCAATCCAAACTATTAAGTGGGTCTAGTTTGGAAGAAACAACATATTGTTCGGAGGGTACATGATTGGAGAGAATAATTTGATTAGTTTCAATCTTTGCTTAGCAATTAATACAGATTAGGGCAGATTCTTGTCATTTCTGGTGGGGAGTATGCTCTCTTGTATGATCACGGGTTGGTGTATTTATTTGTATAATGGGGCTTTGAAGTTCTAGTAACACGAAGAGGATTTAGACCACGTTTTGGGATTGTGATGTTATTACCTCTCTGTGGGATCAGTTTTTTCAAGACTTCTGGGGTTGCAAGCTCGTAACAGTGGCTGTTGTTTGATGTTTGAGGTGCTCTTGAACTCTCCTTTCCATGAAAAAGGAAGGGTTCTTCGGCTGCGGGGCATTTGGCTTGAGAGGAACTCAAGAGTTTTTTGTGGGGTGGAGAGGTTTGGGGAGGATCTTTGGGATTTGATTAGATGTAATTCCTCTTTATGGGCAATAGTTTTCTAATGCTGTTTACACAAATTAAGTGTGTACTTCGTAGTATTTATTCAAGATTTTCTTTAAGTCTCATGCTTAAGTTTGAATGTAACACTGCATTTATGTCTCCACAGTCAACAAGATGGAGGAGATGCAGTTCTTTCAAGAGAAAATAAGAGGAGATTGTCCCAGGTGAAGTTCTAAAATGGCAGTTCTTAGTGAAATATACTATAATTGAAAAACTGTAGCTCCAATAACTATCGATCTGTCTACGAAAAAATCAACAAAGTGTTTCAAATTTTGAAATGACTTTTTTCCTTTGTACTGCCATTGAGTTCTCTTTCCAGTCAAGATCTCTGTCTCATGATTTGAAACAAGTTTTATTTTGGGTACTGTGTTCTTAATCTTAACTATTTGCAATGTTGTTTTCAATTATAATGGTTATTTAAAATATTCAGTGGTTTCTGTTTCTTATGAAAAAAAGGAGAATAATAAATATATTGAATTTGTTTGATGAACGAATTTATTCTGCCCTTTGCAGTATTTACGCCATATTCCGCAGTTTGTGAAGATTGTTGAAGTCGGTCCAAGAGATGGACTACAAAATGAAAAGCACATTGTACCAACTGCTGTTAAGGTTGAATTAATAAAGATGCTAGTCTCTTCTGGGTTGCGTATTGTTGAAGCCACGAGTTTTGTTTCTCCTAAATGGGTACCTCAGGTAACTTGGCTTGATAGTCAATGAACATGTTGGTTGGTATATTTTTGTGTTCCTAAAACAATTACTTCAACTTTTCATGCACTCTGCCTTCATGTTTGAGTTTCTTGTAGATATACTAATTAATCTCGTTTCCTCTTATTTTCCTCAGTTAGCAGATGCAAACGATGTTATGGAGGGAATTCGAAATGTTGAAGGTGCTCGATTTCCTGTGTTGACCCCAAATATTAAGGTATGAACTTCAGTACAGAAATATAAAGGTAGGACTAATCTGGAATGCATTTTGTGATTATTGATGAGATTTTTTGACTGCTTTGTATAGGGTTTTGAAGCGGCTGTTGCAGCTGGAGCAAAGGAAGTAGCTATCTTTGCTTCAGCATCCGAATCCTTCTCAAAGTCAAATATCAATTGCAGCATAGAAGAAAGTCTCCGTCGTTACCGTGAAGTTGCTGTTGCTGCAAGAAAGCACTCACTTCCAGTTCGCGGGTAGGTACTTTGAACCAGAAAACTGAGTTACTATAACATATCTGATATCTGGTTTGATTTCGTATAAACATGTTAGATATGATTTGATCCTCCCCTGGTTTGATATGATTTACTTTTTATATTTTGAAAATGTCTCCTATAAACATGTTAGATATGTATCATGTGTTGTGGGCTGTCCCATTGAAGGAATGGTGCCCCCATCAAATGTAGCATACGTTGCTAAGGAGCTTTACAGTATGGGATGCTCTGAAATATCCCTTGGCGATACAATTGGTGTTGGTACTCCAGGTAACCTCTTGGAATAGCTTTAGTTTAAAATCACAGAGGATATGAACAGAATCTATCTTCTGATGGCATTTGGACAAACCGAAGAAAGATTATATCGCACGTTAAATTAGGTATCTGTATGGTCTATGCCTCTCATTCTAAATTGGTTAACTTATATCTTTCATTGCTAGCATGATGTAAGTACTTCATTATAGGATTTCCTTTGTATATCCTTTGTAATATTAGTTATGTATTATGTGTATGTTTCATATTTATACCGGTGTCTTCTTTATTCATTAATGGATTTTCTTCTTATATAAGAGGACCTTGTATCTCCATAGTTTCTTAATTAGAAGAGTTCCCATGGTGAACTCAATTTTTCCTAAACAATTATTTAACAATGATTCTTTTAAATATTTGTTTTAAGAATCCTTTTTTGACAGTAATTTTTCTAACTCTTCCAGGAGTCATTGGCAACATTCATGTTCTTGTTAACTTTAGTTTTCTCACCTCTGCAGGAACTGTGATTCCAATGCTTGAATCTGTAGTTGATGTTGTTCCAATTGAAAATCTTGCTGTTCATTTCCATGACACTTATGGGCAAGCTCTGTCCAATATCCTGCTCTCACTCCAGGTAATCTCTGTTTTTTCCATCTTGGTTTTAATTGGTTGGAACCTACTAGTTTAAAAAAAAATATATATGCCAATGAGTTGGTATTTATTGGCGAGTATCAAAGTATAATTTAGGGAAATTAGAGACCTTATCTCTCCTGCTCTACCTTGCAGTTAGCAAGAATACTTGTTGTAACACCCCAAGCCCACCACTAGCTGATATTGTTCTCTTTGAGCTTTTCCTTTCGGGCTTCCCCTCAAGGTTTTTAAAACGTGTCTAATAGGGAGAGGTTTCCACACCCTTATAATGGATGCTTCGTTCTCCTCCCCAACCAACGTGGGATCTCACTCTTATGCCCCAATCTCTTTCTGACGCCCTCTTTACTCCTAGGCAACCTGCTCACATAACTAACTTGCAGTGGCCACTGTCCTATTGTTTGCGAAATTCAATTTTATGTTTAATAGATCTTTTATTGATAAAAGTTCCAGGAAAAGATTTTCAATTTAACCAATAATATACTAGTAACTGATGTGTCAAATAATGAAGAGGAGTAGGTACTAACATAGTACTAAATTTTTCTCCTTTCACTTGCTTCCTCTTGCATGTAGACTACCAAGGGCCTGCCATTATCTCTTGGCTTCTAAGACTATCTTGAATTTGATTAAGAGAAAAATATGTATGAAGTACCTAATTACAACATATAAATATACACGCGCGCGCGCGCACACACACACACACACACATATATATATATATATATATATATATATATATATTAAAGAAGCATTATTCTAAGTTGAGGGCTACCATGCTAAGATTTCATTTGATGACCATTTGATTTTGGTTTCGAAAACTAAGCTTATGAGTTTTTTCACTCTATTTGATCAACTGTTATAATGAAAACGAATCTGTTACATGCAATGGATATAATGATGTTGAAAACGATACAAAAGATGAAGATGAAGAAAAACAGCTTATCTTATTAACAGATGGGGATTGGCACGGTGGATTGTTCGGTTTCTGGCCTAGGGGGTTGCCCATATGCTAAAGGTGCTTCTGGAAACGTTGCAACTGAAGATGTTGTGTATATGCTGAATGGAATTGGAGTGAAGACCAACGTAGATCTTGAAAAGCTCATGTTGGCTGGAGATTTCATCTCCAAGCACCTGGGGCGTCCTTCCGCTTCAAAAGTAGCAATTGCATTGCAGAAAGTTGCCTCCAAGCCCAATAAATGCACAGCTACTGCATCCAAATTATGAGGTACTTTACACTATATTCTTGCGTCTTCTCTGATATTGTTCCTCAAGACCCCTAGTAGCTTTCTTCTAAGTTTTAGGTGCAAAAGTATTTTATCATGTATGTTGCTCTCATATAACCCGAAACGAAAGCATTTATGAGTGAATAACTGAGCAGCCTTACCTACAGAAAAAGGCCGCAAGTCGAGTAAAATAAGACCAAACACGTGAGCGGAATACGAGACGAGGCCAAACCTCAAAGGGGTCTCTCAAACCCTCTGAATAATGAGCATGTTAGCCAATCCAATCATATTGACACAACTTATCATTCTGTTTAGGTGAAATCAACATTTCCTCTTCATCTTATCAAGGGCAATGTCTGTGAAGAAAACTCTTGCTTTCAATACTTAGTAGCATTGTGAATGCAGAGAATGAAGCGTCGAAGAGATGCACGGTCTCGATGGCAGATGGGCATATGAAGAAATAATGGAGCACGGTCTCGTTAGTGTATTGATGAAAACCATTTATCTACACCGATCACCACCTTCTTTCATTAACTTTCTCATGTTTCAATCTACCGCTTAACATTTCTCTATTTTTTTTTTAACATTCTCTTTAATCCTCTCTCCTCTTCTTCAATTCTCCAATGTTTAAAGTTACCTGTTCAATCGAAATATATAAATTATAGCAAGAAGAAAAAGAAT

mRNA sequence

TGATCTTGATCTTCATTCTTGTTTATTGCTTTAGGACGATGCAAGCTGCAAAGGTTTCTGCTAAATTGACTCGACGGGGTTTGTCTTGGCGCCATGGATATAATCCAACCATGAGAATTTCCAGCCGGTTTCTTAGTTCATCTTCGGCTTCGCATGTAGGCGTCACCTCTACTAACGGTCTCGGCATACAAGGAGAAGTTGATAATTGCTGATTAGGGCAGATTCTTGTCATTTCTGGTGGGGAGTATGCTCTCTTGTATGATCACGGTCAACAAGATGGAGGAGATGCAGTTCTTTCAAGAGAAAATAAGAGGAGATTGTCCCAGTATTTACGCCATATTCCGCAGTTTGTGAAGATTGTTGAAGTCGGTCCAAGAGATGGACTACAAAATGAAAAGCACATTGTACCAACTGCTGTTAAGGTTGAATTAATAAAGATGCTAGTCTCTTCTGGGTTGCGTATTGTTGAAGCCACGAGTTTTGTTTCTCCTAAATGGGTACCTCAGTTAGCAGATGCAAACGATGTTATGGAGGGAATTCGAAATGTTGAAGGTGCTCGATTTCCTGTGTTGACCCCAAATATTAAGGGTTTTGAAGCGGCTGTTGCAGCTGGAGCAAAGGAAGTAGCTATCTTTGCTTCAGCATCCGAATCCTTCTCAAAGTCAAATATCAATTGCAGCATAGAAGAAAGTCTCCGTCGTTACCGTGAAGTTGCTGTTGCTGCAAGAAAGCACTCACTTCCAGTTCGCGGATATGATTTGATCCTCCCCTGGTTTGATATGATTTACTTTTTATATTTTGAAAATGTCTCCTATAAACATGTTAGATATGTATCATGTGTTGTGGGCTGTCCCATTGAAGGAATGGTGCCCCCATCAAATGTAGCATACGTTGCTAAGGAGCTTTACAGTATGGGATGCTCTGAAATATCCCTTGGCGATACAATTGGTGTTGGTACTCCAGGAGTCATTGGCAACATTCATGTTCTTGTTAACTTTAGTTTTCTCACCTCTGCAGGAACTGTGATTCCAATGCTTGAATCTGTAGTTGATGTTGTTCCAATTGAAAATCTTGCTGTTCATTTCCATGACACTTATGGGCAAGCTCTGTCCAATATCCTGCTCTCACTCCAGATGGGGATTGGCACGGTGGATTGTTCGGTTTCTGGCCTAGGGGGTTGCCCATATGCTAAAGGTGCTTCTGGAAACGTTGCAACTGAAGATGTTGTGTATATGCTGAATGGAATTGGAGTGAAGACCAACGTAGATCTTGAAAAGCTCATGTTGGCTGGAGATTTCATCTCCAAGCACCTGGGGCGTCCTTCCGCTTCAAAAGTAGCAATTGCATTGCAGAAAGTTGCCTCCAAGCCCAATAAATGCACAGCTACTGCATCCAAATTATGAGGTGAAATCAACATTTCCTCTTCATCTTATCAAGGGCAATGTCTGTGAAGAAAACTCTTGCTTTCAATACTTAGTAGCATTGTGAATGCAGAGAATGAAGCGTCGAAGAGATGCACGGTCTCGATGGCAGATGGGCATATGAAGAAATAATGGAGCACGGTCTCGTTAGTGTATTGATGAAAACCATTTATCTACACCGATCACCACCTTCTTTCATTAACTTTCTCATGTTTCAATCTACCGCTTAACATTTCTCTATTTTTTTTTTAACATTCTCTTTAATCCTCTCTCCTCTTCTTCAATTCTCCAATGTTTAAAGTTACCTGTTCAATCGAAATATATAAATTATAGCAAGAAGAAAAAGAAT

Coding sequence (CDS)

ATGCTAGTCTCTTCTGGGTTGCGTATTGTTGAAGCCACGAGTTTTGTTTCTCCTAAATGGGTACCTCAGTTAGCAGATGCAAACGATGTTATGGAGGGAATTCGAAATGTTGAAGGTGCTCGATTTCCTGTGTTGACCCCAAATATTAAGGGTTTTGAAGCGGCTGTTGCAGCTGGAGCAAAGGAAGTAGCTATCTTTGCTTCAGCATCCGAATCCTTCTCAAAGTCAAATATCAATTGCAGCATAGAAGAAAGTCTCCGTCGTTACCGTGAAGTTGCTGTTGCTGCAAGAAAGCACTCACTTCCAGTTCGCGGATATGATTTGATCCTCCCCTGGTTTGATATGATTTACTTTTTATATTTTGAAAATGTCTCCTATAAACATGTTAGATATGTATCATGTGTTGTGGGCTGTCCCATTGAAGGAATGGTGCCCCCATCAAATGTAGCATACGTTGCTAAGGAGCTTTACAGTATGGGATGCTCTGAAATATCCCTTGGCGATACAATTGGTGTTGGTACTCCAGGAGTCATTGGCAACATTCATGTTCTTGTTAACTTTAGTTTTCTCACCTCTGCAGGAACTGTGATTCCAATGCTTGAATCTGTAGTTGATGTTGTTCCAATTGAAAATCTTGCTGTTCATTTCCATGACACTTATGGGCAAGCTCTGTCCAATATCCTGCTCTCACTCCAGATGGGGATTGGCACGGTGGATTGTTCGGTTTCTGGCCTAGGGGGTTGCCCATATGCTAAAGGTGCTTCTGGAAACGTTGCAACTGAAGATGTTGTGTATATGCTGAATGGAATTGGAGTGAAGACCAACGTAGATCTTGAAAAGCTCATGTTGGCTGGAGATTTCATCTCCAAGCACCTGGGGCGTCCTTCCGCTTCAAAAGTAGCAATTGCATTGCAGAAAGTTGCCTCCAAGCCCAATAAATGCACAGCTACTGCATCCAAATTATGA

Protein sequence

MLVSSGLRIVEATSFVSPKWVPQLADANDVMEGIRNVEGARFPVLTPNIKGFEAAVAAGAKEVAIFASASESFSKSNINCSIEESLRRYREVAVAARKHSLPVRGYDLILPWFDMIYFLYFENVSYKHVRYVSCVVGCPIEGMVPPSNVAYVAKELYSMGCSEISLGDTIGVGTPGVIGNIHVLVNFSFLTSAGTVIPMLESVVDVVPIENLAVHFHDTYGQALSNILLSLQMGIGTVDCSVSGLGGCPYAKGASGNVATEDVVYMLNGIGVKTNVDLEKLMLAGDFISKHLGRPSASKVAIALQKVASKPNKCTATASKL

Homology

[Close]

BLAST of Cp4.1LG03g08300 vs. Swiss-Prot
Match: HMGCL_ARATH (Hydroxymethylglutaryl-CoA lyase, mitochondrial OS=Arabidopsis thaliana GN=HMGCL PE=1 SV=2)

HSP 1 Score: 395.2 bits (1014), Expect = 6.8e-109
Identity = 211/320 (65.94%), Postives = 241/320 (75.31%), Query Frame = 1


Query: 2   LVSSGLRIVEATSFVSPKWVPQLADANDVMEGIRNVEGARFPVLTPNIKGFEAAVAAGAK 61
           LVSSGL +VEATSFVSPKWVPQLADA DVM+ +  ++GAR PVLTPN+KGF+AAV+AGAK
Sbjct: 198 LVSSGLPVVEATSFVSPKWVPQLADAKDVMDAVNTLDGARLPVLTPNLKGFQAAVSAGAK 257

Query: 62  EVAIFASASESFSKSNINCSIEESLRRYREVAVAARKHSLPVRGYDLILPWFDMIYFLYF 121
           EVAIFASASESFS SNINC+IEESL RYR VA AA++HS+PVRGY               
Sbjct: 258 EVAIFASASESFSLSNINCTIEESLLRYRVVATAAKEHSVPVRGY--------------- 317

Query: 122 ENVSYKHVRYVSCVVGCPIEGMVPPSNVAYVAKELYSMGCSEISLGDTIGVGTPGVIGNI 181
                     VSCVVGCP+EG V PS VAYV KELY MGC EISLGDTIG+GTP      
Sbjct: 318 ----------VSCVVGCPVEGPVLPSKVAYVVKELYDMGCFEISLGDTIGIGTP------ 377

Query: 182 HVLVNFSFLTSAGTVIPMLESVVDVVPIENLAVHFHDTYGQALSNILLSLQMGIGTVDCS 241
                       G+V+PMLE+V+ VVP + LAVHFHDTYGQAL+NIL+SLQMGI  VD S
Sbjct: 378 ------------GSVVPMLEAVMAVVPADKLAVHFHDTYGQALANILVSLQMGISIVDSS 437

Query: 242 VSGLGGCPYAKGASGNVATEDVVYMLNGIGVKTNVDLEKLMLAGDFISKHLGRPSASKVA 301
           ++GLGGCPYAKGASGNVATEDVVYMLNG+GV TNVDL KL+ AGDFISKHLGRP+ SK A
Sbjct: 438 IAGLGGCPYAKGASGNVATEDVVYMLNGLGVHTNVDLGKLIAAGDFISKHLGRPNGSKAA 468

Query: 302 IALQKVASKPNKCTATASKL 322
           +AL +      + TA ASK+
Sbjct: 498 VALNR------RITADASKI 468

[Close]

BLAST of Cp4.1LG03g08300 vs. Swiss-Prot
Match: HMGCL_RAT (Hydroxymethylglutaryl-CoA lyase, mitochondrial OS=Rattus norvegicus GN=Hmgcl PE=2 SV=1)

HSP 1 Score: 320.1 bits (819), Expect = 2.8e-86
Identity = 171/307 (55.70%), Postives = 214/307 (69.71%), Query Frame = 1


Query: 1   MLVSSGLRIVEATSFVSPKWVPQLADANDVMEGIRNVEGARFPVLTPNIKGFEAAVAAGA 60
           ML  +GL ++EATSFVSPKWVPQ+AD +DV++GI+   G  +PVLTPN+KGFE AVAAGA
Sbjct: 62  MLSEAGLPVIEATSFVSPKWVPQMADHSDVLKGIQKFPGINYPVLTPNMKGFEEAVAAGA 121

Query: 61  KEVAIFASASESFSKSNINCSIEESLRRYREVAVAARKHSLPVRGYDLILPWFDMIYFLY 120
           KEV+IF +ASE F++ N+NCSIEES +R+  V  AAR  S+ VRGY              
Sbjct: 122 KEVSIFGAASELFTRKNVNCSIEESFQRFDGVMQAARAASISVRGY-------------- 181

Query: 121 FENVSYKHVRYVSCVVGCPIEGMVPPSNVAYVAKELYSMGCSEISLGDTIGVGTPGVIGN 180
                      VSC +GCP EG V P+ VA VAK+LYSMGC EISLGDTIGVGTPG++ +
Sbjct: 182 -----------VSCALGCPYEGKVSPAKVAEVAKKLYSMGCYEISLGDTIGVGTPGLMKD 241

Query: 181 IHVLVNFSFLTSAGTVIPMLESVVDVVPIENLAVHFHDTYGQALSNILLSLQMGIGTVDC 240
                             ML +V+  VP+  LAVH HDTYGQAL+N L++LQMG+  VD 
Sbjct: 242 ------------------MLTAVLHEVPVAALAVHCHDTYGQALANTLVALQMGVSVVDS 301

Query: 241 SVSGLGGCPYAKGASGNVATEDVVYMLNGIGVKTNVDLEKLMLAGDFISKHLGRPSASKV 300
           SV+GLGGCPYAKGASGN+ATED+VYML G+G+ T V+L+KL+ AGDFI + L R ++SKV
Sbjct: 302 SVAGLGGCPYAKGASGNLATEDLVYMLTGLGIHTGVNLQKLLEAGDFICQALNRKTSSKV 325

Query: 301 AIALQKV 308
           A A  K+
Sbjct: 362 AQATCKL 325

[Close]

BLAST of Cp4.1LG03g08300 vs. Swiss-Prot
Match: HMGCL_CHICK (Hydroxymethylglutaryl-CoA lyase, mitochondrial OS=Gallus gallus GN=HMGCL PE=1 SV=1)

HSP 1 Score: 318.5 bits (815), Expect = 8.1e-86
Identity = 170/303 (56.11%), Postives = 210/303 (69.31%), Query Frame = 1


Query: 1   MLVSSGLRIVEATSFVSPKWVPQLADANDVMEGIRNVEGARFPVLTPNIKGFEAAVAAGA 60
           ML  +GL ++EATSFVSP+WVPQ+AD  +VM+GI  + G  +PVLTPN+KGF+AAVAAGA
Sbjct: 35  MLSETGLPVIEATSFVSPRWVPQMADHAEVMQGINKLPGVSYPVLTPNLKGFQAAVAAGA 94

Query: 61  KEVAIFASASESFSKSNINCSIEESLRRYREVAVAARKHSLPVRGYDLILPWFDMIYFLY 120
           KEV+IF +ASE F+K NINCSIEESL R+ EV  AAR  S+PVRG               
Sbjct: 95  KEVSIFGAASELFTKKNINCSIEESLERFSEVMNAARAASIPVRG--------------- 154

Query: 121 FENVSYKHVRYVSCVVGCPIEGMVPPSNVAYVAKELYSMGCSEISLGDTIGVGTPGVIGN 180
                     YVSCV+GCP EG +  + VA V+K++YSMGC EISLGD IG+GTP     
Sbjct: 155 ----------YVSCVLGCPYEGNISAAKVAEVSKKMYSMGCYEISLGDRIGIGTP----- 214

Query: 181 IHVLVNFSFLTSAGTVIPMLESVVDVVPIENLAVHFHDTYGQALSNILLSLQMGIGTVDC 240
                        G++  ML +V+  VP+  LAVH HDTYGQAL+NIL++LQMG+  VD 
Sbjct: 215 -------------GSMKEMLAAVMKEVPVGALAVHCHDTYGQALANILVALQMGVSVVDA 274

Query: 241 SVSGLGGCPYAKGASGNVATEDVVYMLNGIGVKTNVDLEKLMLAGDFISKHLGRPSASKV 300
           SV+GLGGCPYA+GASGNVATED+VYMLNG+G+ T VDL+KLM  G FI   L R + SKV
Sbjct: 275 SVAGLGGCPYAQGASGNVATEDLVYMLNGLGIHTGVDLQKLMDTGTFICNALNRRTNSKV 294

Query: 301 AIA 304
           + A
Sbjct: 335 SQA 294

[Close]

BLAST of Cp4.1LG03g08300 vs. Swiss-Prot
Match: HMGCL_MOUSE (Hydroxymethylglutaryl-CoA lyase, mitochondrial OS=Mus musculus GN=Hmgcl PE=1 SV=2)

HSP 1 Score: 317.8 bits (813), Expect = 1.4e-85
Identity = 169/307 (55.05%), Postives = 213/307 (69.38%), Query Frame = 1


Query: 1   MLVSSGLRIVEATSFVSPKWVPQLADANDVMEGIRNVEGARFPVLTPNIKGFEAAVAAGA 60
           ML  +GL ++EATSFVSPKWVPQ+AD +DV++GI+   G  +PVLTPN+KGFE AVAAGA
Sbjct: 62  MLSEAGLPVIEATSFVSPKWVPQMADHSDVLKGIQKFPGINYPVLTPNMKGFEEAVAAGA 121

Query: 61  KEVAIFASASESFSKSNINCSIEESLRRYREVAVAARKHSLPVRGYDLILPWFDMIYFLY 120
           KEV++F + SE F++ N NCSIEES +R+  V  AA+  S+ VRGY              
Sbjct: 122 KEVSVFGAVSELFTRKNANCSIEESFQRFAGVMQAAQAASISVRGY-------------- 181

Query: 121 FENVSYKHVRYVSCVVGCPIEGMVPPSNVAYVAKELYSMGCSEISLGDTIGVGTPGVIGN 180
                      VSC +GCP EG V P+ VA VAK+LYSMGC EISLGDTIGVGTPG++ +
Sbjct: 182 -----------VSCALGCPYEGKVSPAKVAEVAKKLYSMGCYEISLGDTIGVGTPGLMKD 241

Query: 181 IHVLVNFSFLTSAGTVIPMLESVVDVVPIENLAVHFHDTYGQALSNILLSLQMGIGTVDC 240
                             ML +V+  VP+  LAVH HDTYGQAL+N L++LQMG+  VD 
Sbjct: 242 ------------------MLTAVMHEVPVTALAVHCHDTYGQALANTLVALQMGVSVVDS 301

Query: 241 SVSGLGGCPYAKGASGNVATEDVVYMLNGIGVKTNVDLEKLMLAGDFISKHLGRPSASKV 300
           SV+GLGGCPYAKGASGN+ATED+VYMLNG+G+ T V+L+KL+ AGDFI + L R ++SKV
Sbjct: 302 SVAGLGGCPYAKGASGNLATEDLVYMLNGLGIHTGVNLQKLLEAGDFICQALNRKTSSKV 325

Query: 301 AIALQKV 308
           A A  K+
Sbjct: 362 AQATCKL 325

[Close]

BLAST of Cp4.1LG03g08300 vs. Swiss-Prot
Match: HMGCL_PONAB (Hydroxymethylglutaryl-CoA lyase, mitochondrial OS=Pongo abelii GN=HMGCL PE=2 SV=1)

HSP 1 Score: 313.9 bits (803), Expect = 2.0e-84
Identity = 165/307 (53.75%), Postives = 210/307 (68.40%), Query Frame = 1


Query: 1   MLVSSGLRIVEATSFVSPKWVPQLADANDVMEGIRNVEGARFPVLTPNIKGFEAAVAAGA 60
           ML  +GL ++E TSFVSPKWVPQ+ D  +V++GI+   G  +PVLTPN+KGFEAAVAAGA
Sbjct: 62  MLSEAGLSVIETTSFVSPKWVPQMGDHTEVLKGIQKFPGINYPVLTPNLKGFEAAVAAGA 121

Query: 61  KEVAIFASASESFSKSNINCSIEESLRRYREVAVAARKHSLPVRGYDLILPWFDMIYFLY 120
           KEVAIF +ASE F+K NINCSIEES +R+  +  AA+  ++ VRGY              
Sbjct: 122 KEVAIFGAASELFTKKNINCSIEESFQRFDAILKAAQSANISVRGY-------------- 181

Query: 121 FENVSYKHVRYVSCVVGCPIEGMVPPSNVAYVAKELYSMGCSEISLGDTIGVGTPGVIGN 180
                      VSC +GCP EG + P+ VA V K+LYSMGC EISLGDTIGVGTPG++  
Sbjct: 182 -----------VSCALGCPYEGKISPAKVAEVTKKLYSMGCYEISLGDTIGVGTPGIMKG 241

Query: 181 IHVLVNFSFLTSAGTVIPMLESVVDVVPIENLAVHFHDTYGQALSNILLSLQMGIGTVDC 240
                             ML +V+  VP+  LAVH HDTYGQAL+N L++LQMG+  VD 
Sbjct: 242 ------------------MLSAVMQEVPLAALAVHCHDTYGQALANTLMALQMGVSVVDS 301

Query: 241 SVSGLGGCPYAKGASGNVATEDVVYMLNGIGVKTNVDLEKLMLAGDFISKHLGRPSASKV 300
           SV+GLGGCPYA+GASGN+ATED+VYML G+G+ T V+L+KL+ AG+FI + L R ++SKV
Sbjct: 302 SVAGLGGCPYAQGASGNLATEDLVYMLEGLGIHTGVNLQKLLEAGNFICQALNRKTSSKV 325

Query: 301 AIALQKV 308
           A A  K+
Sbjct: 362 AQATCKL 325

[Close]

BLAST of Cp4.1LG03g08300 vs. TrEMBL
Match: A0A0A0LHM6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G823050 PE=4 SV=1)

HSP 1 Score: 472.6 bits (1215), Expect = 3.7e-130
Identity = 257/321 (80.06%), Postives = 264/321 (82.24%), Query Frame = 1


Query: 1   MLVSSGLRIVEATSFVSPKWVPQLADANDVMEGIRNVEGARFPVLTPNIKGFEAAVAAGA 60
           MLVSSGL +VEATSFVSPKWVPQLADA +VMEGIRNVEGARFPVLTPN+KGFEAAVAAGA
Sbjct: 115 MLVSSGLPVVEATSFVSPKWVPQLADAKEVMEGIRNVEGARFPVLTPNLKGFEAAVAAGA 174

Query: 61  KEVAIFASASESFSKSNINCSIEESLRRYREVAVAARKHSLPVRGYDLILPWFDMIYFLY 120
           KEVAIFASASESFSKSNINCSIEESL RYR+VA AARKHSLPVRGY              
Sbjct: 175 KEVAIFASASESFSKSNINCSIEESLHRYRQVADAARKHSLPVRGY-------------- 234

Query: 121 FENVSYKHVRYVSCVVGCPIEGMVPPSNVAYVAKELYSMGCSEISLGDTIGVGTPGVIGN 180
                      VSCVVGCPIEGMVP SNVAYVAKELYSMGCSEISLGDTIGVGTP     
Sbjct: 235 -----------VSCVVGCPIEGMVPASNVAYVAKELYSMGCSEISLGDTIGVGTP----- 294

Query: 181 IHVLVNFSFLTSAGTVIPMLESVVDVVPIENLAVHFHDTYGQALSNILLSLQMGIGTVDC 240
                        GTVIPMLESV +VVP ENLAVHFHDTYGQALSNIL+SLQMGIGTVD 
Sbjct: 295 -------------GTVIPMLESVANVVPTENLAVHFHDTYGQALSNILVSLQMGIGTVDS 354

Query: 241 SVSGLGGCPYAKGASGNVATEDVVYMLNGIGVKTNVDLEKLMLAGDFISKHLGRPSASKV 300
           SVSGLGGCPYAKGASGNVATEDVVYMLNGIG KTNVDL KLMLAGDFI K LGRPSASKV
Sbjct: 355 SVSGLGGCPYAKGASGNVATEDVVYMLNGIGAKTNVDLGKLMLAGDFICKQLGRPSASKV 392

Query: 301 AIALQKVASKPNKCTATASKL 322
           AIAL+KVAS  NKCTATASKL
Sbjct: 415 AIALRKVASNTNKCTATASKL 392

[Close]

BLAST of Cp4.1LG03g08300 vs. TrEMBL
Match: V4UVP8_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10011898mg PE=4 SV=1)

HSP 1 Score: 424.5 bits (1090), Expect = 1.2e-115
Identity = 228/321 (71.03%), Postives = 251/321 (78.19%), Query Frame = 1


Query: 1   MLVSSGLRIVEATSFVSPKWVPQLADANDVMEGIRNVEGARFPVLTPNIKGFEAAVAAGA 60
           +LVSSGL +VEATSFVSPKWVPQLADA DVM  I+NVEGARFPVLTPN+KGFEAAVAAGA
Sbjct: 127 LLVSSGLPVVEATSFVSPKWVPQLADAKDVMAAIQNVEGARFPVLTPNLKGFEAAVAAGA 186

Query: 61  KEVAIFASASESFSKSNINCSIEESLRRYREVAVAARKHSLPVRGYDLILPWFDMIYFLY 120
           KEVAIFASASESFSKSNINC+IE+SL RYR+VA+AAR+ S+PVRGY              
Sbjct: 187 KEVAIFASASESFSKSNINCTIEDSLIRYRDVALAARELSIPVRGY-------------- 246

Query: 121 FENVSYKHVRYVSCVVGCPIEGMVPPSNVAYVAKELYSMGCSEISLGDTIGVGTPGVIGN 180
                      +SCVVGCP+EGMVPPS VAYV+K+LY MGCSEISLGDTIGVGTP     
Sbjct: 247 -----------LSCVVGCPVEGMVPPSKVAYVSKQLYDMGCSEISLGDTIGVGTP----- 306

Query: 181 IHVLVNFSFLTSAGTVIPMLESVVDVVPIENLAVHFHDTYGQALSNILLSLQMGIGTVDC 240
                        GTVIPMLE+V+D VP++ LAVHFHDTYGQALSNIL SLQMGI TVD 
Sbjct: 307 -------------GTVIPMLEAVLDAVPVDKLAVHFHDTYGQALSNILASLQMGISTVDS 366

Query: 241 SVSGLGGCPYAKGASGNVATEDVVYMLNGIGVKTNVDLEKLMLAGDFISKHLGRPSASKV 300
           SVSGLGGCPYAKGASGNVATEDVVYMLNG+GV+TNVD+ KLM+AGDFI KHLGR S SK 
Sbjct: 367 SVSGLGGCPYAKGASGNVATEDVVYMLNGLGVRTNVDIRKLMIAGDFICKHLGRTSGSKT 398

Query: 301 AIALQKVASKPNKCTATASKL 322
           AIAL K +      TA ASKL
Sbjct: 427 AIALSKTS------TANASKL 398

[Close]

BLAST of Cp4.1LG03g08300 vs. TrEMBL
Match: A0A061EVW1_THECC (Aldolase superfamily protein OS=Theobroma cacao GN=TCM_023924 PE=4 SV=1)

HSP 1 Score: 423.3 bits (1087), Expect = 2.6e-115
Identity = 222/304 (73.03%), Postives = 245/304 (80.59%), Query Frame = 1


Query: 1   MLVSSGLRIVEATSFVSPKWVPQLADANDVMEGIRNVEGARFPVLTPNIKGFEAAVAAGA 60
           MLVSSGL +VEATSFVSPKWVPQLADA DVM+ I+N++G RFPVLTPN+KGFEAAVAAGA
Sbjct: 124 MLVSSGLTVVEATSFVSPKWVPQLADAKDVMDAIQNIQGGRFPVLTPNLKGFEAAVAAGA 183

Query: 61  KEVAIFASASESFSKSNINCSIEESLRRYREVAVAARKHSLPVRGYDLILPWFDMIYFLY 120
           KEVAIFASASESFSKSNINCSI++SL RYR+VA AAR+ SLPVRGY              
Sbjct: 184 KEVAIFASASESFSKSNINCSIKDSLARYRDVASAARELSLPVRGY-------------- 243

Query: 121 FENVSYKHVRYVSCVVGCPIEGMVPPSNVAYVAKELYSMGCSEISLGDTIGVGTPGVIGN 180
                      +SCVVGCP+EG VPPS VAYVAK+L+ MGCSEISLGDTIGVGTP     
Sbjct: 244 -----------ISCVVGCPVEGTVPPSKVAYVAKQLFDMGCSEISLGDTIGVGTP----- 303

Query: 181 IHVLVNFSFLTSAGTVIPMLESVVDVVPIENLAVHFHDTYGQALSNILLSLQMGIGTVDC 240
                        GTVIPMLE+V+ VVPI+ LAVHFHDTYGQALSNIL+SLQMGI TVD 
Sbjct: 304 -------------GTVIPMLENVIHVVPIDKLAVHFHDTYGQALSNILISLQMGISTVDS 363

Query: 241 SVSGLGGCPYAKGASGNVATEDVVYMLNGIGVKTNVDLEKLMLAGDFISKHLGRPSASKV 300
           SVSGLGGCPYAKGASGNVATEDVVYMLNG+GV+T+VDLEKLMLAGDFI KHLGRPS+SK 
Sbjct: 364 SVSGLGGCPYAKGASGNVATEDVVYMLNGLGVETHVDLEKLMLAGDFICKHLGRPSSSKT 384

Query: 301 AIAL 305
           A+AL
Sbjct: 424 AVAL 384

[Close]

BLAST of Cp4.1LG03g08300 vs. TrEMBL
Match: F6HQN4_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_08s0040g01170 PE=4 SV=1)

HSP 1 Score: 421.8 bits (1083), Expect = 7.6e-115
Identity = 227/313 (72.52%), Postives = 242/313 (77.32%), Query Frame = 1


Query: 1   MLVSSGLRIVEATSFVSPKWVPQLADANDVMEGIRNVEGARFPVLTPNIKGFEAAVAAGA 60
           MLVSSGL IVEATSFVSPKWVPQLADA DVME IR VEG RFPVLTPN+KGFEAAVAAGA
Sbjct: 126 MLVSSGLPIVEATSFVSPKWVPQLADAKDVMEAIRTVEGGRFPVLTPNLKGFEAAVAAGA 185

Query: 61  KEVAIFASASESFSKSNINCSIEESLRRYREVAVAARKHSLPVRGYDLILPWFDMIYFLY 120
           KEVAIFASASESFSKSNINCSIE+SL RYR+VA+AARK S+PVRGY              
Sbjct: 186 KEVAIFASASESFSKSNINCSIEDSLTRYRDVALAARKLSIPVRGY-------------- 245

Query: 121 FENVSYKHVRYVSCVVGCPIEGMVPPSNVAYVAKELYSMGCSEISLGDTIGVGTPGVIGN 180
                      +SCVVGCP+EG+V PS V YV KELY MGC EISLGDTIGVGTPG    
Sbjct: 246 -----------ISCVVGCPLEGVVSPSKVTYVGKELYDMGCYEISLGDTIGVGTPG---- 305

Query: 181 IHVLVNFSFLTSAGTVIPMLESVVDVVPIENLAVHFHDTYGQALSNILLSLQMGIGTVDC 240
                         TVIPMLE V+DVVPI+ LAVHFHDTYGQALSNIL SLQMGI TVD 
Sbjct: 306 --------------TVIPMLEDVIDVVPIDKLAVHFHDTYGQALSNILASLQMGITTVDS 365

Query: 241 SVSGLGGCPYAKGASGNVATEDVVYMLNGIGVKTNVDLEKLMLAGDFISKHLGRPSASKV 300
           SVSGLGGCPYAKGASGNVATEDVVYMLNG+GVKTNVDL KLMLAGDFI +HLGR S SK 
Sbjct: 366 SVSGLGGCPYAKGASGNVATEDVVYMLNGLGVKTNVDLGKLMLAGDFICQHLGRQSGSKT 395

Query: 301 AIALQKVASKPNK 314
           AIAL ++    +K
Sbjct: 426 AIALSRIRGHASK 395

[Close]

BLAST of Cp4.1LG03g08300 vs. TrEMBL
Match: W9SJS1_9ROSA (Hydroxymethylglutaryl-CoA lyase OS=Morus notabilis GN=L484_014678 PE=4 SV=1)

HSP 1 Score: 419.5 bits (1077), Expect = 3.8e-114
Identity = 228/320 (71.25%), Postives = 253/320 (79.06%), Query Frame = 1


Query: 2   LVSSGLRIVEATSFVSPKWVPQLADANDVMEGIRNVEGARFPVLTPNIKGFEAAVAAGAK 61
           LVSSGLR++EA+SFVSPKWVPQLADA DVME IR+VEGARFPVLTPN+KGFEAAVAAGAK
Sbjct: 90  LVSSGLRVIEASSFVSPKWVPQLADAKDVMEAIRDVEGARFPVLTPNLKGFEAAVAAGAK 149

Query: 62  EVAIFASASESFSKSNINCSIEESLRRYREVAVAARKHSLPVRGYDLILPWFDMIYFLYF 121
           EVAIFASASESFSKSNINCSIE+SL RYR+VA+AA+K ++PVRGY               
Sbjct: 150 EVAIFASASESFSKSNINCSIEDSLIRYRDVALAAKKLTIPVRGY--------------- 209

Query: 122 ENVSYKHVRYVSCVVGCPIEGMVPPSNVAYVAKELYSMGCSEISLGDTIGVGTPGVIGNI 181
                     +SCVVGCP+EG VPPS VA+VAKELY+MGCSEISLGDTIGVGTP      
Sbjct: 210 ----------ISCVVGCPVEGKVPPSQVAHVAKELYAMGCSEISLGDTIGVGTP------ 269

Query: 182 HVLVNFSFLTSAGTVIPMLESVVDVVPIENLAVHFHDTYGQALSNILLSLQMGIGTVDCS 241
                       GTVIPMLE+V D VPI+ LAVHFHDTYGQALSN+L+SLQ+GI TVD S
Sbjct: 270 ------------GTVIPMLEAVTDAVPIKKLAVHFHDTYGQALSNMLVSLQVGITTVDSS 329

Query: 242 VSGLGGCPYAKGASGNVATEDVVYMLNGIGVKTNVDLEKLMLAGDFISKHLGRPSASKVA 301
           VSGLGGCPYAKGASGNVA+EDVVYMLNG+GVKTNVDL KL+LAG+FIS HLGR S SKVA
Sbjct: 330 VSGLGGCPYAKGASGNVASEDVVYMLNGLGVKTNVDLGKLILAGEFISNHLGRSSGSKVA 358

Query: 302 IALQKVASKPNKCTATASKL 322
           IAL+K     N C   ASKL
Sbjct: 390 IALRK-----NTC---ASKL 358

[Close]

BLAST of Cp4.1LG03g08300 vs. TAIR10
Match: AT2G26800.2 (AT2G26800.2 Aldolase superfamily protein)

HSP 1 Score: 395.2 bits (1014), Expect = 3.8e-110
Identity = 211/320 (65.94%), Postives = 241/320 (75.31%), Query Frame = 1


Query: 2   LVSSGLRIVEATSFVSPKWVPQLADANDVMEGIRNVEGARFPVLTPNIKGFEAAVAAGAK 61
           LVSSGL +VEATSFVSPKWVPQLADA DVM+ +  ++GAR PVLTPN+KGF+AAV+AGAK
Sbjct: 198 LVSSGLPVVEATSFVSPKWVPQLADAKDVMDAVNTLDGARLPVLTPNLKGFQAAVSAGAK 257

Query: 62  EVAIFASASESFSKSNINCSIEESLRRYREVAVAARKHSLPVRGYDLILPWFDMIYFLYF 121
           EVAIFASASESFS SNINC+IEESL RYR VA AA++HS+PVRGY               
Sbjct: 258 EVAIFASASESFSLSNINCTIEESLLRYRVVATAAKEHSVPVRGY--------------- 317

Query: 122 ENVSYKHVRYVSCVVGCPIEGMVPPSNVAYVAKELYSMGCSEISLGDTIGVGTPGVIGNI 181
                     VSCVVGCP+EG V PS VAYV KELY MGC EISLGDTIG+GTP      
Sbjct: 318 ----------VSCVVGCPVEGPVLPSKVAYVVKELYDMGCFEISLGDTIGIGTP------ 377

Query: 182 HVLVNFSFLTSAGTVIPMLESVVDVVPIENLAVHFHDTYGQALSNILLSLQMGIGTVDCS 241
                       G+V+PMLE+V+ VVP + LAVHFHDTYGQAL+NIL+SLQMGI  VD S
Sbjct: 378 ------------GSVVPMLEAVMAVVPADKLAVHFHDTYGQALANILVSLQMGISIVDSS 437

Query: 242 VSGLGGCPYAKGASGNVATEDVVYMLNGIGVKTNVDLEKLMLAGDFISKHLGRPSASKVA 301
           ++GLGGCPYAKGASGNVATEDVVYMLNG+GV TNVDL KL+ AGDFISKHLGRP+ SK A
Sbjct: 438 IAGLGGCPYAKGASGNVATEDVVYMLNGLGVHTNVDLGKLIAAGDFISKHLGRPNGSKAA 468

Query: 302 IALQKVASKPNKCTATASKL 322
           +AL +      + TA ASK+
Sbjct: 498 VALNR------RITADASKI 468

[Close]

BLAST of Cp4.1LG03g08300 vs. NCBI nr
Match: gi|659085281|ref|XP_008443338.1| (PREDICTED: hydroxymethylglutaryl-CoA lyase, mitochondrial-like [Cucumis melo])

HSP 1 Score: 479.2 bits (1232), Expect = 5.7e-132
Identity = 259/321 (80.69%), Postives = 266/321 (82.87%), Query Frame = 1


Query: 1   MLVSSGLRIVEATSFVSPKWVPQLADANDVMEGIRNVEGARFPVLTPNIKGFEAAVAAGA 60
           MLV SGL +VEATSFVSPKWVPQLADA DVMEGIRNVEGARFPVLTPN+KGFEAAVAAGA
Sbjct: 111 MLVFSGLPVVEATSFVSPKWVPQLADAKDVMEGIRNVEGARFPVLTPNLKGFEAAVAAGA 170

Query: 61  KEVAIFASASESFSKSNINCSIEESLRRYREVAVAARKHSLPVRGYDLILPWFDMIYFLY 120
           KEVAIFASASESFSKSNINCSIEESL RYR+VA AARKHSLPVRGY              
Sbjct: 171 KEVAIFASASESFSKSNINCSIEESLHRYRQVADAARKHSLPVRGY-------------- 230

Query: 121 FENVSYKHVRYVSCVVGCPIEGMVPPSNVAYVAKELYSMGCSEISLGDTIGVGTPGVIGN 180
                      VSCVVGCPIEGMVP SNVAYVAKELYSMGCSEISLGDTIGVGTP     
Sbjct: 231 -----------VSCVVGCPIEGMVPASNVAYVAKELYSMGCSEISLGDTIGVGTP----- 290

Query: 181 IHVLVNFSFLTSAGTVIPMLESVVDVVPIENLAVHFHDTYGQALSNILLSLQMGIGTVDC 240
                        GTVIPMLESV++VVPIENLAVHFHDTYGQALSNIL+SLQMGIGTVD 
Sbjct: 291 -------------GTVIPMLESVINVVPIENLAVHFHDTYGQALSNILVSLQMGIGTVDS 350

Query: 241 SVSGLGGCPYAKGASGNVATEDVVYMLNGIGVKTNVDLEKLMLAGDFISKHLGRPSASKV 300
           SVSGLGGCPYAKGASGNVATEDVVYMLNGIG KTNVDLEKLMLAGDFI KHLGRPSASKV
Sbjct: 351 SVSGLGGCPYAKGASGNVATEDVVYMLNGIGAKTNVDLEKLMLAGDFICKHLGRPSASKV 388

Query: 301 AIALQKVASKPNKCTATASKL 322
           AIAL+KV S  NKCTATASKL
Sbjct: 411 AIALRKVTSNANKCTATASKL 388

[Close]

BLAST of Cp4.1LG03g08300 vs. NCBI nr
Match: gi|778685384|ref|XP_011652210.1| (PREDICTED: hydroxymethylglutaryl-CoA lyase, mitochondrial [Cucumis sativus])

HSP 1 Score: 472.6 bits (1215), Expect = 5.4e-130
Identity = 257/321 (80.06%), Postives = 264/321 (82.24%), Query Frame = 1


Query: 1   MLVSSGLRIVEATSFVSPKWVPQLADANDVMEGIRNVEGARFPVLTPNIKGFEAAVAAGA 60
           MLVSSGL +VEATSFVSPKWVPQLADA +VMEGIRNVEGARFPVLTPN+KGFEAAVAAGA
Sbjct: 115 MLVSSGLPVVEATSFVSPKWVPQLADAKEVMEGIRNVEGARFPVLTPNLKGFEAAVAAGA 174

Query: 61  KEVAIFASASESFSKSNINCSIEESLRRYREVAVAARKHSLPVRGYDLILPWFDMIYFLY 120
           KEVAIFASASESFSKSNINCSIEESL RYR+VA AARKHSLPVRGY              
Sbjct: 175 KEVAIFASASESFSKSNINCSIEESLHRYRQVADAARKHSLPVRGY-------------- 234

Query: 121 FENVSYKHVRYVSCVVGCPIEGMVPPSNVAYVAKELYSMGCSEISLGDTIGVGTPGVIGN 180
                      VSCVVGCPIEGMVP SNVAYVAKELYSMGCSEISLGDTIGVGTP     
Sbjct: 235 -----------VSCVVGCPIEGMVPASNVAYVAKELYSMGCSEISLGDTIGVGTP----- 294

Query: 181 IHVLVNFSFLTSAGTVIPMLESVVDVVPIENLAVHFHDTYGQALSNILLSLQMGIGTVDC 240
                        GTVIPMLESV +VVP ENLAVHFHDTYGQALSNIL+SLQMGIGTVD 
Sbjct: 295 -------------GTVIPMLESVANVVPTENLAVHFHDTYGQALSNILVSLQMGIGTVDS 354

Query: 241 SVSGLGGCPYAKGASGNVATEDVVYMLNGIGVKTNVDLEKLMLAGDFISKHLGRPSASKV 300
           SVSGLGGCPYAKGASGNVATEDVVYMLNGIG KTNVDL KLMLAGDFI K LGRPSASKV
Sbjct: 355 SVSGLGGCPYAKGASGNVATEDVVYMLNGIGAKTNVDLGKLMLAGDFICKQLGRPSASKV 392

Query: 301 AIALQKVASKPNKCTATASKL 322
           AIAL+KVAS  NKCTATASKL
Sbjct: 415 AIALRKVASNTNKCTATASKL 392

[Close]

BLAST of Cp4.1LG03g08300 vs. NCBI nr
Match: gi|470104426|ref|XP_004288605.1| (PREDICTED: hydroxymethylglutaryl-CoA lyase, mitochondrial-like [Fragaria vesca subsp. vesca])

HSP 1 Score: 426.4 bits (1095), Expect = 4.4e-116
Identity = 227/313 (72.52%), Postives = 245/313 (78.27%), Query Frame = 1


Query: 1   MLVSSGLRIVEATSFVSPKWVPQLADANDVMEGIRNVEGARFPVLTPNIKGFEAAVAAGA 60
           MLVSSGL +VEATSFVSPKWVPQLADA DVME IRN+ GARFPVLTPN+KGFEAAV AGA
Sbjct: 69  MLVSSGLTVVEATSFVSPKWVPQLADAKDVMEAIRNMGGARFPVLTPNLKGFEAAVDAGA 128

Query: 61  KEVAIFASASESFSKSNINCSIEESLRRYREVAVAARKHSLPVRGYDLILPWFDMIYFLY 120
           KEVAIFASASESFSKSNINCSIE+SLRRYR+VA AARK S+PVRGY              
Sbjct: 129 KEVAIFASASESFSKSNINCSIEDSLRRYRDVASAARKLSIPVRGY-------------- 188

Query: 121 FENVSYKHVRYVSCVVGCPIEGMVPPSNVAYVAKELYSMGCSEISLGDTIGVGTPGVIGN 180
                      +SCVVGCP EG VPPS VAYVAKELY MGC +ISLGDTIGVGTPG    
Sbjct: 189 -----------ISCVVGCPTEGAVPPSQVAYVAKELYDMGCFDISLGDTIGVGTPG---- 248

Query: 181 IHVLVNFSFLTSAGTVIPMLESVVDVVPIENLAVHFHDTYGQALSNILLSLQMGIGTVDC 240
                         TV+PML+ V+DVVP ENLAVHFHDTYGQALSNIL+SLQMGI TVD 
Sbjct: 249 --------------TVVPMLKEVIDVVPAENLAVHFHDTYGQALSNILVSLQMGISTVDS 308

Query: 241 SVSGLGGCPYAKGASGNVATEDVVYMLNGIGVKTNVDLEKLMLAGDFISKHLGRPSASKV 300
           SVSGLGGCPYAKGASGNVATEDVVYMLNG+GVKTN+DL+ LMLAGDFI KHLGR S SK 
Sbjct: 309 SVSGLGGCPYAKGASGNVATEDVVYMLNGLGVKTNIDLKTLMLAGDFICKHLGRSSGSKT 338

Query: 301 AIALQKVASKPNK 314
           A+AL KV +  +K
Sbjct: 369 AVALSKVPAHASK 338

[Close]

BLAST of Cp4.1LG03g08300 vs. NCBI nr
Match: gi|568857003|ref|XP_006482060.1| (PREDICTED: hydroxymethylglutaryl-CoA lyase, mitochondrial-like [Citrus sinensis])

HSP 1 Score: 424.9 bits (1091), Expect = 1.3e-115
Identity = 228/321 (71.03%), Postives = 251/321 (78.19%), Query Frame = 1


Query: 1   MLVSSGLRIVEATSFVSPKWVPQLADANDVMEGIRNVEGARFPVLTPNIKGFEAAVAAGA 60
           +LVSSGL +VEATSFVSPKWVPQLADA DVM  I+NVEGARFPVLTPN+KGFEAAVAAGA
Sbjct: 127 LLVSSGLAVVEATSFVSPKWVPQLADAKDVMAAIQNVEGARFPVLTPNLKGFEAAVAAGA 186

Query: 61  KEVAIFASASESFSKSNINCSIEESLRRYREVAVAARKHSLPVRGYDLILPWFDMIYFLY 120
           KEVAIFASASESFSKSNINC+IE+SL RYR+VA+AAR+ S+PVRGY              
Sbjct: 187 KEVAIFASASESFSKSNINCTIEDSLIRYRDVALAARELSIPVRGY-------------- 246

Query: 121 FENVSYKHVRYVSCVVGCPIEGMVPPSNVAYVAKELYSMGCSEISLGDTIGVGTPGVIGN 180
                      +SCVVGCP+EGMVPPS VAYV+K+LY MGCSEISLGDTIGVGTP     
Sbjct: 247 -----------LSCVVGCPVEGMVPPSKVAYVSKQLYDMGCSEISLGDTIGVGTP----- 306

Query: 181 IHVLVNFSFLTSAGTVIPMLESVVDVVPIENLAVHFHDTYGQALSNILLSLQMGIGTVDC 240
                        GTVIPMLE+V+D VP++ LAVHFHDTYGQALSNIL SLQMGI TVD 
Sbjct: 307 -------------GTVIPMLEAVLDAVPVDKLAVHFHDTYGQALSNILASLQMGISTVDS 366

Query: 241 SVSGLGGCPYAKGASGNVATEDVVYMLNGIGVKTNVDLEKLMLAGDFISKHLGRPSASKV 300
           SVSGLGGCPYAKGASGNVATEDVVYMLNG+GV+TNVD+ KLM+AGDFI KHLGR S SK 
Sbjct: 367 SVSGLGGCPYAKGASGNVATEDVVYMLNGLGVRTNVDIRKLMIAGDFICKHLGRTSGSKT 398

Query: 301 AIALQKVASKPNKCTATASKL 322
           AIAL K +      TA ASKL
Sbjct: 427 AIALSKTS------TANASKL 398

[Close]

BLAST of Cp4.1LG03g08300 vs. NCBI nr
Match: gi|567875879|ref|XP_006430529.1| (hypothetical protein CICLE_v10011898mg [Citrus clementina])

HSP 1 Score: 424.5 bits (1090), Expect = 1.7e-115
Identity = 228/321 (71.03%), Postives = 251/321 (78.19%), Query Frame = 1


Query: 1   MLVSSGLRIVEATSFVSPKWVPQLADANDVMEGIRNVEGARFPVLTPNIKGFEAAVAAGA 60
           +LVSSGL +VEATSFVSPKWVPQLADA DVM  I+NVEGARFPVLTPN+KGFEAAVAAGA
Sbjct: 127 LLVSSGLPVVEATSFVSPKWVPQLADAKDVMAAIQNVEGARFPVLTPNLKGFEAAVAAGA 186

Query: 61  KEVAIFASASESFSKSNINCSIEESLRRYREVAVAARKHSLPVRGYDLILPWFDMIYFLY 120
           KEVAIFASASESFSKSNINC+IE+SL RYR+VA+AAR+ S+PVRGY              
Sbjct: 187 KEVAIFASASESFSKSNINCTIEDSLIRYRDVALAARELSIPVRGY-------------- 246

Query: 121 FENVSYKHVRYVSCVVGCPIEGMVPPSNVAYVAKELYSMGCSEISLGDTIGVGTPGVIGN 180
                      +SCVVGCP+EGMVPPS VAYV+K+LY MGCSEISLGDTIGVGTP     
Sbjct: 247 -----------LSCVVGCPVEGMVPPSKVAYVSKQLYDMGCSEISLGDTIGVGTP----- 306

Query: 181 IHVLVNFSFLTSAGTVIPMLESVVDVVPIENLAVHFHDTYGQALSNILLSLQMGIGTVDC 240
                        GTVIPMLE+V+D VP++ LAVHFHDTYGQALSNIL SLQMGI TVD 
Sbjct: 307 -------------GTVIPMLEAVLDAVPVDKLAVHFHDTYGQALSNILASLQMGISTVDS 366

Query: 241 SVSGLGGCPYAKGASGNVATEDVVYMLNGIGVKTNVDLEKLMLAGDFISKHLGRPSASKV 300
           SVSGLGGCPYAKGASGNVATEDVVYMLNG+GV+TNVD+ KLM+AGDFI KHLGR S SK 
Sbjct: 367 SVSGLGGCPYAKGASGNVATEDVVYMLNGLGVRTNVDIRKLMIAGDFICKHLGRTSGSKT 398

Query: 301 AIALQKVASKPNKCTATASKL 322
           AIAL K +      TA ASKL
Sbjct: 427 AIALSKTS------TANASKL 398

The following BLAST results are available for this feature:

BLAST of Cp4.1LG03g08300 vs. Swiss-Prot


Match Name	E-value	Identity	Description
HMGCL_ARATH	6.8e-109	65.94	Hydroxymethylglutaryl-CoA lyase, mitochondrial OS=Arabidopsis thaliana GN=HMGCL ...	[more]
HMGCL_RAT	2.8e-86	55.70	Hydroxymethylglutaryl-CoA lyase, mitochondrial OS=Rattus norvegicus GN=Hmgcl PE=...	[more]
HMGCL_CHICK	8.1e-86	56.11	Hydroxymethylglutaryl-CoA lyase, mitochondrial OS=Gallus gallus GN=HMGCL PE=1 SV...	[more]
HMGCL_MOUSE	1.4e-85	55.05	Hydroxymethylglutaryl-CoA lyase, mitochondrial OS=Mus musculus GN=Hmgcl PE=1 SV=...	[more]
HMGCL_PONAB	2.0e-84	53.75	Hydroxymethylglutaryl-CoA lyase, mitochondrial OS=Pongo abelii GN=HMGCL PE=2 SV=...	[more]

BLAST of Cp4.1LG03g08300 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LHM6_CUCSA	3.7e-130	80.06	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G823050 PE=4 SV=1	[more]
V4UVP8_9ROSI	1.2e-115	71.03	Uncharacterized protein OS=Citrus clementina GN=CICLE_v10011898mg PE=4 SV=1	[more]
A0A061EVW1_THECC	2.6e-115	73.03	Aldolase superfamily protein OS=Theobroma cacao GN=TCM_023924 PE=4 SV=1	[more]
F6HQN4_VITVI	7.6e-115	72.52	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_08s0040g01170 PE=4 SV=...	[more]
W9SJS1_9ROSA	3.8e-114	71.25	Hydroxymethylglutaryl-CoA lyase OS=Morus notabilis GN=L484_014678 PE=4 SV=1	[more]

BLAST of Cp4.1LG03g08300 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G26800.2	3.8e-110	65.94	Aldolase superfamily protein	[more]

BLAST of Cp4.1LG03g08300 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659085281\|ref\|XP_008443338.1\|	5.7e-132	80.69	PREDICTED: hydroxymethylglutaryl-CoA lyase, mitochondrial-like [Cucumis melo]	[more]
gi\|778685384\|ref\|XP_011652210.1\|	5.4e-130	80.06	PREDICTED: hydroxymethylglutaryl-CoA lyase, mitochondrial [Cucumis sativus]	[more]
gi\|470104426\|ref\|XP_004288605.1\|	4.4e-116	72.52	PREDICTED: hydroxymethylglutaryl-CoA lyase, mitochondrial-like [Fragaria vesca s...	[more]
gi\|568857003\|ref\|XP_006482060.1\|	1.3e-115	71.03	PREDICTED: hydroxymethylglutaryl-CoA lyase, mitochondrial-like [Citrus sinensis]	[more]
gi\|567875879\|ref\|XP_006430529.1\|	1.7e-115	71.03	hypothetical protein CICLE_v10011898mg [Citrus clementina]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003824	catalytic activity

Vocabulary: **INTERPRO**
Term	Definition
IPR013785	Aldolase_TIM
IPR000891	PYR_CT

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008152	metabolic process
biological_process	GO:0046950	cellular ketone body metabolic process
biological_process	GO:0006550	isoleucine catabolic process
biological_process	GO:0006552	leucine catabolic process
biological_process	GO:0006574	valine catabolic process
biological_process	GO:0046951	ketone body biosynthetic process
cellular_component	GO:0005575	cellular_component
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0016829	lyase activity
molecular_function	GO:0004419	hydroxymethylglutaryl-CoA lyase activity
molecular_function	GO:0003824	catalytic activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG03g08300.1	Cp4.1LG03g08300.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000891	Pyruvate carboxyltransferase	PFAM	PF00682	HMGL-like	coord: 1..288 score: 1.1
IPR000891	Pyruvate carboxyltransferase	PROFILE	PS50991	PYR_CT	coord: 1..282 score: 21
IPR013785	Aldolase-type TIM barrel	GENE3D	G3DSA:3.20.20.70		coord: 2..299 score: 1.6
None	No IPR available	PANTHER	PTHR10277	HOMOCITRATE SYNTHASE-RELATED	coord: 195..313 score: 1.2E-191coord: 1..107 score: 1.2E-191coord: 131..176 score: 1.2E
None	No IPR available	PANTHER	PTHR10277:SF38	SUBFAMILY NOT NAMED	coord: 1..107 score: 1.2E-191coord: 195..313 score: 1.2E-191coord: 131..176 score: 1.2E
None	No IPR available	unknown	SSF51569	Aldolase	coord: 2..304 score: 4.25

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cp4.1LG03g08300	Cucsa.338300	Cucumber (Gy14) v1	cgycpeB0888
Cp4.1LG03g08300	CmaCh14G005560	Cucurbita maxima (Rimu)	cmacpeB283
Cp4.1LG03g08300	CmoCh14G005600	Cucurbita moschata (Rifu)	cmocpeB245
Cp4.1LG03g08300	Cla002934	Watermelon (97103) v1	cpewmB597
Cp4.1LG03g08300	Csa3G823050	Cucumber (Chinese Long) v2	cpecuB597
Cp4.1LG03g08300	MELO3C009815	Melon (DHL92) v3.5.1	cpemeB572
Cp4.1LG03g08300	ClCG10G015600	Watermelon (Charleston Gray)	cpewcgB546
Cp4.1LG03g08300	CSPI03G38410	Wild cucumber (PI 183967)	cpecpiB599
Cp4.1LG03g08300	Lsi03G015400	Bottle gourd (USVL1VR-Ls)	cpelsiB503
Cp4.1LG03g08300	MELO3C009815.2	Melon (DHL92) v3.6.1	cpemedB679
Cp4.1LG03g08300	CsaV3_3G040810	Cucumber (Chinese Long) v3	cpecucB0747
Cp4.1LG03g08300	Bhi11G002434	Wax gourd	cpewgoB0756
Cp4.1LG03g08300	Carg09818	Silver-seed gourd	carcpeB0613

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cp4.1LG03g08300	Cucumber (Gy14) v2	cgybcpeB395
Cp4.1LG03g08300	Silver-seed gourd	carcpeB0444
Cp4.1LG03g08300	Wax gourd	cpewgoB0743
Cp4.1LG03g08300	Cucurbita maxima (Rimu)	cmacpeB682
Cp4.1LG03g08300	Cucurbita maxima (Rimu)	cmacpeB841
Cp4.1LG03g08300	Cucurbita moschata (Rifu)	cmocpeB789