Carg09818 (gene) Silver-seed gourd

NameCarg09818
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionHydroxymethylglutaryl-CoA lyase
LocationCucurbita_argyrosperma_scaffold_023 : 1115549 .. 1120450 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTGGATTTATCCACCTTTGATCTTGATCTTCATTCTTGTTTATTGCTTTAGGACGATGCAAGCTGCAAAGGTTTCTGCTAAATTGACTCGACGGGGTTTGTCTTGGCGCCATGGATATAATCCAACCATGAGAATTTCCAGCCGGTTTCTTAGTTCATCTTCGGCTTCGCATGTAGGCGTCAGCTCCACTAACGGGCTTGGCATACAAGGAGAAGTTGATAATTGCTGGTATGGATGAAATGATTGTTCTTCACAAGTTCGTAATCCAGTATGTATGATCTGATTATAGGGAGAATTAATCATACCTTTTGATGCTTTCGTTTTTTTTTATTAAATCGCTTATCGTTGCAATATCTTTTCGCTGAAAATGTGCGATAGAATTCTTTAAATCCTTATGGGGTGAGTCGTGGAATATGGATCGTGAAATTTGGGCGTTTTTCTTTTACGATTTTACGATGGGAAATCTACTTCGACGAATTTGGGGGAATCCGTTCAAGAGTGGAGCTAATAAGCTTTGGGTAAATGCAATCCAAACTATTATGTGGGTCTAGTTTGGAAGAAACAACATATTGTTCGGAGGGTACATGATTGGAGAGAATAATTTGATTAGTTTCAATCTTTGCTTAGCAATTAGGCCTTTGCAATTAATACAGATTAGGGCAGATTCTTGTCAGCTCTGGTGGGGAGTATGCTCTCTTGTATGATCACGGGTTGGTGTATTTATTTGTATAATGGGGCTTTGAAGTTCTAGTAACACGAAGAGGATTTAGACCACTTGTTTTGGGATTGTGATTTTATTACCTCTCTGTGGGATCAGTTTTTGCAAGACTTCTGGGGTTGCAAGCTCGTAACAGTGGGTGTTGTTCGATGTTTGAGGTGCTCTTGAACTCTCCTTTCCATGAAAAAGGAAGGGTTCTTCGGCTGCGGGGCATTTGGCTTGAGAGGAGCTCGAGTTTTTTGTGGGGTGGAGAGGTTTGGGGAGGATCTTTGGGATTTGATTAGATGTAATTCCTCTTTATGGGCAATATTTTTCTAATGCTGTTTACACAAATTAAGTTTGTACTTTGTAGTATTTATTGAAGATTTTCTTTAAGTCTCATGCTTAAGTTTGAATATAACACTGCATTTATGTCTCCACAGTCAACAAGATGGAGGAGATATAGTTCTTTCAAGAGAAAATAAGAGGAGATTGTCCCAGGTGAAGTTCTAAAATGGCAGTTCCTAGTGAAATATACTATAATTGAAAAACTGTAGCTCCGATAACTATCGATCTGTCTACGAAAAAAAATCAACAAAGGTGTTTCAAATTTTGAAATGACTTTTTTCCTTTGTACTGCCATTGAGTTCTCTTTCCAGTCAAGATCTGTCTCATATGATTTGAAACAAGTTTTATTTTGGGTACTGTGTTCTTAATCTTAACTATTTGCAATGTTATTTTCAATTATAATGGTTATTTAAAATATTCAGCGGTTTCTGTCTCTTATGAAAAAAAGGAGAATAATAAATATATTGAATTTGTCTGATGAACGAATTTTTTCTGCCTTTGCAGTATTTACGCCATATTCCGCAGTTTGTGAAGATTGTTGAAGTTGGTCCAAGAGATGGACTACAAAATGAAAAGCACATTGTACCAACTGCTGTTAAGGTTGAATTAATAAAGATGCTAGTCTCTTCTGGGTTGCGTGTTGTTGAAGCCACGAGTTTTGTTTCTCCTAAATGGGTACCTCAGGTAACTTGGCTTGATAGTCAATGAACATGTTGGTTGGTATATTTTTGTGTTCCTAAAACAATTACTTCAACTTTTCATGCACTCTGCCTTCATGTTTGAGTTTCTTGTAGATATACTAATTAACCTCGTTTCCATATTTTCCTCAGTTAGCAGATGCAAACGATGTTATGGAGGGAATTCGAAATGTTGAAGGTGCTCGATTTCCTGTGTTGACCCCAAATATTAAGGTATGAACTTCAGTACAGAAATATAAAGGTAGGACTAATCTGGAATGCATTTTGTGATTATTGATGAGATTTTTTGACTGCTTTGTATAGGGTTTTGAAGCGGCTGTTGCAGCTGGAGCAAAGGAAGTAGCTATCTTTGCTTCAGCATCCGAATCCTTCTCAAAGTCAAATATCAATTGCAGCATAGAAGAAAGTCTCCGTCGTTACCGTGAAGTTGCTGTTGCTGCAAGAAAGCACTCACTTCCAGTTCGCGGGTAGGTACTCTGAACCAGAAAACTGAGTTATTATATCATATCTGATATCTGGTTTGATTTCATATAAACATGTTAGATATGATTTGATACTCCCCTGGTTTGATATGATTTACTTTTTATATCTTGAAAATGTCTCCTATAAACATGTTAGATATGTATCATGTGTTGTGGGCTGTCCCATTGAAGGAATGGTGCCCCCATCAAATGTAGCATACGTCGCTAAGGAGCTTTACGGCATGGGATGCTCTGAAATTTCCCTTGGCGATACAATTGGTGTTGGTACTCCAGGTAACCTCTTGGAATAGCTTTAGTTTAAAATCACAGTGGATATGAACAGAATCTATCTTCGGAAGGCATTTGGACAAACCGAAGAAAGATTATATCGCACGTTAAATTAGGTATCTGTATGGTCTATGCCTCTCATTCTAAATTGGTTAACTTATATCATTCATTGCTAGCATGTTGTAAGTACTCCATTATAGGATTTCCTTTGTATATCTTTTGTAATATTAGTTCTGTATTATGTGTATATTTCATATTTATACCGGTGTCTTCTTTATTTATTAATGGTTTTTCTTCTTATATAAGAGGACCTTGTATCTCCATAGTTTCTTAATTAGAAGAGTTCCCATGGTGAACTCAATTTTTCCTAAACAATTTTTTAACGATGATTTTTTTAAATATTTGTTTTAAGAATCCTTTTTTGACAGTAATTTTTCTAACTCTTCCAGGAGTCATTGGCAACATTCATGTTCTTGTTAACTTTAGTTTTCTCACCTCTGCAGGAACCGTGATTCCAATGCTTGAATCTGTAGTTGATGTTGTTCCAATTGAAAATCTTGCTGTTCATTTCCATGACACTTATGGGCAAGCTCTGTCCAATATCCTACTCTCACTCCAGGTAAACTCTGTTTTTTCCATCTTGGTTTTAATTGGTTGGAACCTACTAGTTTCAAAAAAATATATGCCAATGAGTTGGTATTTATTCGCGAGTATCAAAGTATAATTTAGGGAAATCAGAGACCTTATCTCTCCTGCTCTACCTTGCACTTAGCGAGAATACTTGTTGTAATAGTCTAAGTCCATCGCTAGCAGATATTGTTCTTTTTGGGCTTTCCCTTTCGGGCTTCTCCTTAAGATTTTTAAAACGCGTCTACTAAGAAGAGATTTTCACACCCTTATAATGAATGTTTCGTTCTCCTCCCCAACCGACATGGGATCTCACTCTTATACCCAAACTCTTTCTGATGCCCTCTTTACTCCTAGGCAACCTACTCACATAACTAACTTGCAGTGGCCCACTGTCCTATTATTCACGAAATTCAATTTTATGTTTCATAGATCTTTTGTTGAAAAGTTCCGGGACAAGATTTTCAATTTAACCAATAATATACTAGTAACTGATGTGTCAAATAATGAAGAGGAGTAGGTACTAACAACTAAAAATTTTCTCCTTTCACTTACTTCCTCTCTTGGCTTCTAAGACTATGTTGAATTTGATTAAGAGAAAAATATGTATGAAGTACTTAATTACAACATATATATATATATATATATATATATATATATATATATATATATATATATATACGCGCACGTACATATATATCTAGTTTGTCAACTTAGAGAGCCCAAAAAGACAAAATGAAGACCGTGTTGTCCTAGAATTTTCAAACTTAGAACAACTAGATTTTCTTAAAGAAGCATTATTCTAAGTTGAGGGCTACCGTGCTAAGATTTCATTTGATTTTGGTTTTTGAAAACCAAGCTTATGATTTTTTTCACTCTATTTGATCAACTGTTGTAATGAAAACGAATCTGTTACATGCAATGATGTTGAAAACGATACAAAAGTTGAAGATGAAAATAAACAGCTTATCTTATTAACAGATGGGGATTGGCACGGTGGATTGTTCGGTTTCTGGTCTAGGGGGCTGCCCATATGCTAAAGGTGCTTCTGGAAACGTTGCAACTGAAGATGTTGTGTATATGCTGAATGGAATTGGAGTGAAGACCAACGTAGACCTTGAAAAGCTCGTGTTGGCTGGAGATTTCATCTCCAAGCACCTGGGGCGTCCTTCCGCTTCAAAAGTAGCAATTGCATTGCAGAAAGTTGCCTCCAAGCCCAATAAATGCACAGCTACTGCATCCAAATTATGAGGTACTTTACACTATATTCTTGCGTCTTCTCTGATATTGTTCCTCAAGACCCCTAGTAGCTTTCTTCTAAGTTTTAGGTGCTAAAGTATTTTATCATGTATGTTGCTCTCATATAACCCGAAACGAAAGCATTTATGAGTGAATAACCGAGCAACCTACAGAAAAAGGCCTCGAGTCGAGTAAAATAAGACCAAACACGTGAGCGGAATACGAGACGAGGCCAAACCTCAAAGGGGTCTCTCAAACCCTCTGAATAATATTGACACAACTTATCCTTCTGTTTAGGTGAAATGAACATTTCCTGTTCATCCTTATCAAGGGCAATGTCTGTGAAGAAAACTCTTGCTTTCAATACTTAGTAGCATCGTGAATGCACAGAATGAAGCGTCGAAGAGATGCACGGTCTCGATGGCAGATGGGCATATGAAGAAATAATGGAGCACGGTCTCGTTAGTGTATTGATGAAAACCATTTATCTACACCGATCACCACCCTCTTTCATTAAACTTTCTCATGTTTCAATCTACAGC

mRNA sequence

ATGTGGATTTATCCACCTTTGATCTTGATCTTCATTCTTGTTTATTGCTTTAGGACGATGCAAGCTGCAAAGGTTTCTGCTAAATTGACTCGACGGGGTTTGTCTTGGCGCCATGGATATAATCCAACCATGAGAATTTCCAGCCGGTTTCTTAGTTCATCTTCGGCTTCGCATGTAGGCGTCAGCTCCACTAACGGGCTTGGCATACAAGGAGAAGTTGATAATTGCTGTCAACAAGATGGAGGAGATATAGTTCTTTCAAGAGAAAATAAGAGGAGATTGTCCCAGTATTTACGCCATATTCCGCAGTTTGTGAAGATTGTTGAAGTTGGTCCAAGAGATGGACTACAAAATGAAAAGCACATTGTACCAACTGCTGTTAAGGTTGAATTAATAAAGATGCTAGTCTCTTCTGGGTTGCGTGTTGTTGAAGCCACGAGTTTTGTTTCTCCTAAATGGGTACCTCAGTTAGCAGATGCAAACGATGTTATGGAGGGAATTCGAAATGTTGAAGGTGCTCGATTTCCTGTGTTGACCCCAAATATTAAGGGTTTTGAAGCGGCTGTTGCAGCTGGAGCAAAGGAAGTAGCTATCTTTGCTTCAGCATCCGAATCCTTCTCAAAGTCAAATATCAATTGCAGCATAGAAGAAAGTCTCCGTCGTTACCGTGAAGTTGCTGTTGCTGCAAGAAAGCACTCACTTCCAGTTCGCGGATATGTATCATGTGTTGTGGGCTGTCCCATTGAAGGAATGGTGCCCCCATCAAATGTAGCATACGTCGCTAAGGAGCTTTACGGCATGGGATGCTCTGAAATTTCCCTTGGCGATACAATTGGTGTTGGTACTCCAGGAACCGTGATTCCAATGCTTGAATCTGTAGTTGATGTTGTTCCAATTGAAAATCTTGCTGTTCATTTCCATGACACTTATGGGCAAGCTCTGTCCAATATCCTACTCTCACTCCAGATGGGGATTGGCACGGTGGATTGTTCGGTTTCTGGTCTAGGGGGCTGCCCATATGCTAAAGGTGCTTCTGGAAACGTTGCAACTGAAGATGTTGTGTATATGCTGAATGGAATTGGAGTGAAGACCAACGTAGACCTTGAAAAGCTCGTGTTGGCTGGAGATTTCATCTCCAAGCACCTGGGGCGTCCTTCCGCTTCAAAAGTAGCAATTGCATTGCAGAAAGTTGCCTCCAAGCCCAATAAATGCACAGCTACTGCATCCAAATTATGAGGTACTTTACACTATATTCTTGCGTCTTCTCTGATATTGTTCCTCAAGACCCCTAGTAGCTTTCTTCTAAGTTTTAGGTGCTAAAGTATTTTATCATGTATGTTGCTCTCATATAACCCGAAACGAAAGCATTTATGAGTGAATAACCGAGCAACCTACAGAAAAAGGCCTCGAGTCGAGTAAAATAAGACCAAACACGTGAGCGGAATACGAGACGAGGCCAAACCTCAAAGGGGTCTCTCAAACCCTCTGAATAATATTGACACAACTTATCCTTCTGTTTAGGTGAAATGAACATTTCCTGTTCATCCTTATCAAGGGCAATGTCTGTGAAGAAAACTCTTGCTTTCAATACTTAGTAGCATCGTGAATGCACAGAATGAAGCGTCGAAGAGATGCACGGTCTCGATGGCAGATGGGCATATGAAGAAATAATGGAGCACGGTCTCGTTAGTGTATTGATGAAAACCATTTATCTACACCGATCACCACCCTCTTTCATTAAACTTTCTCATGTTTCAATCTACAGC

Coding sequence (CDS)

ATGTGGATTTATCCACCTTTGATCTTGATCTTCATTCTTGTTTATTGCTTTAGGACGATGCAAGCTGCAAAGGTTTCTGCTAAATTGACTCGACGGGGTTTGTCTTGGCGCCATGGATATAATCCAACCATGAGAATTTCCAGCCGGTTTCTTAGTTCATCTTCGGCTTCGCATGTAGGCGTCAGCTCCACTAACGGGCTTGGCATACAAGGAGAAGTTGATAATTGCTGTCAACAAGATGGAGGAGATATAGTTCTTTCAAGAGAAAATAAGAGGAGATTGTCCCAGTATTTACGCCATATTCCGCAGTTTGTGAAGATTGTTGAAGTTGGTCCAAGAGATGGACTACAAAATGAAAAGCACATTGTACCAACTGCTGTTAAGGTTGAATTAATAAAGATGCTAGTCTCTTCTGGGTTGCGTGTTGTTGAAGCCACGAGTTTTGTTTCTCCTAAATGGGTACCTCAGTTAGCAGATGCAAACGATGTTATGGAGGGAATTCGAAATGTTGAAGGTGCTCGATTTCCTGTGTTGACCCCAAATATTAAGGGTTTTGAAGCGGCTGTTGCAGCTGGAGCAAAGGAAGTAGCTATCTTTGCTTCAGCATCCGAATCCTTCTCAAAGTCAAATATCAATTGCAGCATAGAAGAAAGTCTCCGTCGTTACCGTGAAGTTGCTGTTGCTGCAAGAAAGCACTCACTTCCAGTTCGCGGATATGTATCATGTGTTGTGGGCTGTCCCATTGAAGGAATGGTGCCCCCATCAAATGTAGCATACGTCGCTAAGGAGCTTTACGGCATGGGATGCTCTGAAATTTCCCTTGGCGATACAATTGGTGTTGGTACTCCAGGAACCGTGATTCCAATGCTTGAATCTGTAGTTGATGTTGTTCCAATTGAAAATCTTGCTGTTCATTTCCATGACACTTATGGGCAAGCTCTGTCCAATATCCTACTCTCACTCCAGATGGGGATTGGCACGGTGGATTGTTCGGTTTCTGGTCTAGGGGGCTGCCCATATGCTAAAGGTGCTTCTGGAAACGTTGCAACTGAAGATGTTGTGTATATGCTGAATGGAATTGGAGTGAAGACCAACGTAGACCTTGAAAAGCTCGTGTTGGCTGGAGATTTCATCTCCAAGCACCTGGGGCGTCCTTCCGCTTCAAAAGTAGCAATTGCATTGCAGAAAGTTGCCTCCAAGCCCAATAAATGCACAGCTACTGCATCCAAATTATGA

Protein sequence

MWIYPPLILIFILVYCFRTMQAAKVSAKLTRRGLSWRHGYNPTMRISSRFLSSSSASHVGVSSTNGLGIQGEVDNCCQQDGGDIVLSRENKRRLSQYLRHIPQFVKIVEVGPRDGLQNEKHIVPTAVKVELIKMLVSSGLRVVEATSFVSPKWVPQLADANDVMEGIRNVEGARFPVLTPNIKGFEAAVAAGAKEVAIFASASESFSKSNINCSIEESLRRYREVAVAARKHSLPVRGYVSCVVGCPIEGMVPPSNVAYVAKELYGMGCSEISLGDTIGVGTPGTVIPMLESVVDVVPIENLAVHFHDTYGQALSNILLSLQMGIGTVDCSVSGLGGCPYAKGASGNVATEDVVYMLNGIGVKTNVDLEKLVLAGDFISKHLGRPSASKVAIALQKVASKPNKCTATASKL
BLAST of Carg09818 vs. NCBI nr
Match: XP_022934490.1 (hydroxymethylglutaryl-CoA lyase, mitochondrial-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 689.1 bits (1777), Expect = 9.1e-195
Identity = 390/392 (99.49%), Postives = 392/392 (100.00%), Query Frame = 0

Query: 20  MQAAKVSAKLTRRGLSWRHGYNPTMRISSRFLSSSSASHVGVSSTNGLGIQGEVDNCCQQ 79
           MQAAKVSAKLTRRGLSWRHGYNPTMRISSRFLSSSSASHVGVSSTNGLGIQGEVDNCCQQ
Sbjct: 1   MQAAKVSAKLTRRGLSWRHGYNPTMRISSRFLSSSSASHVGVSSTNGLGIQGEVDNCCQQ 60

Query: 80  DGGDIVLSRENKRRLSQYLRHIPQFVKIVEVGPRDGLQNEKHIVPTAVKVELIKMLVSSG 139
           DGGD+VLSRENKRRLSQYLRHIPQFVKIVEVGPRDGLQNEKHIVPTAVKVELIKMLVSSG
Sbjct: 61  DGGDVVLSRENKRRLSQYLRHIPQFVKIVEVGPRDGLQNEKHIVPTAVKVELIKMLVSSG 120

Query: 140 LRVVEATSFVSPKWVPQLADANDVMEGIRNVEGARFPVLTPNIKGFEAAVAAGAKEVAIF 199
           LRVVEATSFVSPKWVPQLADANDVMEGIRNVEGARFPVLTPNIKGFEAAVAAGAKEVAIF
Sbjct: 121 LRVVEATSFVSPKWVPQLADANDVMEGIRNVEGARFPVLTPNIKGFEAAVAAGAKEVAIF 180

Query: 200 ASASESFSKSNINCSIEESLRRYREVAVAARKHSLPVRGYVSCVVGCPIEGMVPPSNVAY 259
           ASASESFSKSNINCSIEESLRRYREVAVAARKHSLPVRGYVSCVVGCPIEGMVPPSNVAY
Sbjct: 181 ASASESFSKSNINCSIEESLRRYREVAVAARKHSLPVRGYVSCVVGCPIEGMVPPSNVAY 240

Query: 260 VAKELYGMGCSEISLGDTIGVGTPGTVIPMLESVVDVXXXXXXXXXXXXXXXXXXXXXXX 319
           VAKELYGMGCSEISLGDTIGVGTPGTVIPMLESVVDVXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 241 VAKELYGMGCSEISLGDTIGVGTPGTVIPMLESVVDVXXXXXXXXXXXXXXXXXXXXXXX 300

Query: 320 XXXXXXXXVDCSVSGLGGCPYAKGASGNVATEDVVYMLNGIGVKTNVDLEKLVLAGDFIS 379
           XXXXXXXXVDCSVSGLGGCPYAKGASGNVATEDVVYMLNGIGVKTNVDLEKL+LAGDFIS
Sbjct: 301 XXXXXXXXVDCSVSGLGGCPYAKGASGNVATEDVVYMLNGIGVKTNVDLEKLMLAGDFIS 360

Query: 380 KHLGRPSASKVAIALQKVASKPNKCTATASKL 412
           KHLGRPSASKVAIALQKVASKPNKCTATASKL
Sbjct: 361 KHLGRPSASKVAIALQKVASKPNKCTATASKL 392

BLAST of Carg09818 vs. NCBI nr
Match: XP_023526450.1 (hydroxymethylglutaryl-CoA lyase, mitochondrial-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023526451.1 hydroxymethylglutaryl-CoA lyase, mitochondrial-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 683.7 bits (1763), Expect = 3.8e-193
Identity = 356/392 (90.82%), Postives = 359/392 (91.58%), Query Frame = 0

Query: 20  MQAAKVSAKLTRRGLSWRHGYNPTMRISSRFLSSSSASHVGVSSTNGLGIQGEVDNCCQQ 79
           MQAAKVSAKLTRRGLSWRHGYNPTMRISSRFLSSSSASHVGV+STNGLGIQGEVDNCCQQ
Sbjct: 1   MQAAKVSAKLTRRGLSWRHGYNPTMRISSRFLSSSSASHVGVTSTNGLGIQGEVDNCCQQ 60

Query: 80  DGGDIVLSRENKRRLSQYLRHIPQFVKIVEVGPRDGLQNEKHIVPTAVKVELIKMLVSSG 139
           DGGD VLSRENKRRLSQYLRHIPQFVKIVEVGPRDGLQNEKHIVPTAVKVELIKMLVSSG
Sbjct: 61  DGGDAVLSRENKRRLSQYLRHIPQFVKIVEVGPRDGLQNEKHIVPTAVKVELIKMLVSSG 120

Query: 140 LRVVEATSFVSPKWVPQLADANDVMEGIRNVEGARFPVLTPNIKGFEAAVAAGAKEVAIF 199
           LR+VEATSFVSPKWVPQLADANDVMEGIRNVEGARFPVLTPNIKGFEAAVAAGAKEVAIF
Sbjct: 121 LRIVEATSFVSPKWVPQLADANDVMEGIRNVEGARFPVLTPNIKGFEAAVAAGAKEVAIF 180

Query: 200 ASASESFSKSNINCSIEESLRRYREVAVAARKHSLPVRGYVSCVVGCPIEGMVPPSNVAY 259
           ASASESFSKSNINCSIEESLRRYREVAVAARKHSLPVRGYVSCVVGCPIEGMVPPSNVAY
Sbjct: 181 ASASESFSKSNINCSIEESLRRYREVAVAARKHSLPVRGYVSCVVGCPIEGMVPPSNVAY 240

Query: 260 VAKELYGMGCSEISLGDTIGVGTPGTVIPMLESVVDVXXXXXXXXXXXXXXXXXXXXXXX 319
           VAKELY MGCSEISLGDTIGVGTPGTVIPMLESVVDV                       
Sbjct: 241 VAKELYSMGCSEISLGDTIGVGTPGTVIPMLESVVDVVPIENLAVHFHDTYGQALSNILL 300

Query: 320 XXXXXXXXVDCSVSGLGGCPYAKGASGNVATEDVVYMLNGIGVKTNVDLEKLVLAGDFIS 379
                   VDCSVSGLGGCPYAKGASGNVATEDVVYMLNGIGVKTNVDLEKL+LAGDFIS
Sbjct: 301 SLQMGIGTVDCSVSGLGGCPYAKGASGNVATEDVVYMLNGIGVKTNVDLEKLMLAGDFIS 360

Query: 380 KHLGRPSASKVAIALQKVASKPNKCTATASKL 412
           KHLGRPSASKVAIALQKVASKPNKCTATASKL
Sbjct: 361 KHLGRPSASKVAIALQKVASKPNKCTATASKL 392

BLAST of Carg09818 vs. NCBI nr
Match: XP_022983793.1 (hydroxymethylglutaryl-CoA lyase, mitochondrial-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 679.1 bits (1751), Expect = 9.4e-192
Identity = 355/392 (90.56%), Postives = 357/392 (91.07%), Query Frame = 0

Query: 20  MQAAKVSAKLTRRGLSWRHGYNPTMRISSRFLSSSSASHVGVSSTNGLGIQGEVDNCCQQ 79
           MQAAKVSAKLTRRGLSWRHGYNPTMRISSRFLSSSSASHVGVSSTNGLGIQGEV NCCQQ
Sbjct: 2   MQAAKVSAKLTRRGLSWRHGYNPTMRISSRFLSSSSASHVGVSSTNGLGIQGEVHNCCQQ 61

Query: 80  DGGDIVLSRENKRRLSQYLRHIPQFVKIVEVGPRDGLQNEKHIVPTAVKVELIKMLVSSG 139
           DGGD VLSRENKRRLSQYLRHIPQFVKIVEVGPRDGLQNEKHIVPTAVKVELIKMLVSSG
Sbjct: 62  DGGDAVLSRENKRRLSQYLRHIPQFVKIVEVGPRDGLQNEKHIVPTAVKVELIKMLVSSG 121

Query: 140 LRVVEATSFVSPKWVPQLADANDVMEGIRNVEGARFPVLTPNIKGFEAAVAAGAKEVAIF 199
           LR+VEATSFVSPKWVPQLADANDVMEGIRNVEGARFPVLTPNIKGFEAAVAAGAKEVAIF
Sbjct: 122 LRIVEATSFVSPKWVPQLADANDVMEGIRNVEGARFPVLTPNIKGFEAAVAAGAKEVAIF 181

Query: 200 ASASESFSKSNINCSIEESLRRYREVAVAARKHSLPVRGYVSCVVGCPIEGMVPPSNVAY 259
           ASASESFSKSNINCSIEESLRRYREVAVAARK SLPVRGYVSCVVGCPIEGMVPPSNVAY
Sbjct: 182 ASASESFSKSNINCSIEESLRRYREVAVAARKQSLPVRGYVSCVVGCPIEGMVPPSNVAY 241

Query: 260 VAKELYGMGCSEISLGDTIGVGTPGTVIPMLESVVDVXXXXXXXXXXXXXXXXXXXXXXX 319
           VAKELY MGCSEISLGDTIGVGTPGTVIPMLESVVDV                       
Sbjct: 242 VAKELYSMGCSEISLGDTIGVGTPGTVIPMLESVVDVVPIENLAVHFHDTYGQALSNILL 301

Query: 320 XXXXXXXXVDCSVSGLGGCPYAKGASGNVATEDVVYMLNGIGVKTNVDLEKLVLAGDFIS 379
                   VDCSVSGLGGCPYAKGASGNVATEDVVYMLNGIGVKTNVDLEKL+LAGDFIS
Sbjct: 302 SLQMGIGTVDCSVSGLGGCPYAKGASGNVATEDVVYMLNGIGVKTNVDLEKLMLAGDFIS 361

Query: 380 KHLGRPSASKVAIALQKVASKPNKCTATASKL 412
           KHLGRPSASKVAIALQKVASKPNKCTATASKL
Sbjct: 362 KHLGRPSASKVAIALQKVASKPNKCTATASKL 393

BLAST of Carg09818 vs. NCBI nr
Match: XP_022934491.1 (hydroxymethylglutaryl-CoA lyase, mitochondrial-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 575.5 bits (1482), Expect = 1.5e-160
Identity = 332/334 (99.40%), Postives = 334/334 (100.00%), Query Frame = 0

Query: 78  QQDGGDIVLSRENKRRLSQYLRHIPQFVKIVEVGPRDGLQNEKHIVPTAVKVELIKMLVS 137
           QQDGGD+VLSRENKRRLSQYLRHIPQFVKIVEVGPRDGLQNEKHIVPTAVKVELIKMLVS
Sbjct: 14  QQDGGDVVLSRENKRRLSQYLRHIPQFVKIVEVGPRDGLQNEKHIVPTAVKVELIKMLVS 73

Query: 138 SGLRVVEATSFVSPKWVPQLADANDVMEGIRNVEGARFPVLTPNIKGFEAAVAAGAKEVA 197
           SGLRVVEATSFVSPKWVPQLADANDVMEGIRNVEGARFPVLTPNIKGFEAAVAAGAKEVA
Sbjct: 74  SGLRVVEATSFVSPKWVPQLADANDVMEGIRNVEGARFPVLTPNIKGFEAAVAAGAKEVA 133

Query: 198 IFASASESFSKSNINCSIEESLRRYREVAVAARKHSLPVRGYVSCVVGCPIEGMVPPSNV 257
           IFASASESFSKSNINCSIEESLRRYREVAVAARKHSLPVRGYVSCVVGCPIEGMVPPSNV
Sbjct: 134 IFASASESFSKSNINCSIEESLRRYREVAVAARKHSLPVRGYVSCVVGCPIEGMVPPSNV 193

Query: 258 AYVAKELYGMGCSEISLGDTIGVGTPGTVIPMLESVVDVXXXXXXXXXXXXXXXXXXXXX 317
           AYVAKELYGMGCSEISLGDTIGVGTPGTVIPMLESVVDVXXXXXXXXXXXXXXXXXXXXX
Sbjct: 194 AYVAKELYGMGCSEISLGDTIGVGTPGTVIPMLESVVDVXXXXXXXXXXXXXXXXXXXXX 253

Query: 318 XXXXXXXXXXVDCSVSGLGGCPYAKGASGNVATEDVVYMLNGIGVKTNVDLEKLVLAGDF 377
           XXXXXXXXXXVDCSVSGLGGCPYAKGASGNVATEDVVYMLNGIGVKTNVDLEKL+LAGDF
Sbjct: 254 XXXXXXXXXXVDCSVSGLGGCPYAKGASGNVATEDVVYMLNGIGVKTNVDLEKLMLAGDF 313

Query: 378 ISKHLGRPSASKVAIALQKVASKPNKCTATASKL 412
           ISKHLGRPSASKVAIALQKVASKPNKCTATASKL
Sbjct: 314 ISKHLGRPSASKVAIALQKVASKPNKCTATASKL 347

BLAST of Carg09818 vs. NCBI nr
Match: XP_023526452.1 (hydroxymethylglutaryl-CoA lyase, mitochondrial-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 571.2 bits (1471), Expect = 2.8e-159
Identity = 299/334 (89.52%), Postives = 301/334 (90.12%), Query Frame = 0

Query: 78  QQDGGDIVLSRENKRRLSQYLRHIPQFVKIVEVGPRDGLQNEKHIVPTAVKVELIKMLVS 137
           QQDGGD VLSRENKRRLSQYLRHIPQFVKIVEVGPRDGLQNEKHIVPTAVKVELIKMLVS
Sbjct: 14  QQDGGDAVLSRENKRRLSQYLRHIPQFVKIVEVGPRDGLQNEKHIVPTAVKVELIKMLVS 73

Query: 138 SGLRVVEATSFVSPKWVPQLADANDVMEGIRNVEGARFPVLTPNIKGFEAAVAAGAKEVA 197
           SGLR+VEATSFVSPKWVPQLADANDVMEGIRNVEGARFPVLTPNIKGFEAAVAAGAKEVA
Sbjct: 74  SGLRIVEATSFVSPKWVPQLADANDVMEGIRNVEGARFPVLTPNIKGFEAAVAAGAKEVA 133

Query: 198 IFASASESFSKSNINCSIEESLRRYREVAVAARKHSLPVRGYVSCVVGCPIEGMVPPSNV 257
           IFASASESFSKSNINCSIEESLRRYREVAVAARKHSLPVRGYVSCVVGCPIEGMVPPSNV
Sbjct: 134 IFASASESFSKSNINCSIEESLRRYREVAVAARKHSLPVRGYVSCVVGCPIEGMVPPSNV 193

Query: 258 AYVAKELYGMGCSEISLGDTIGVGTPGTVIPMLESVVDVXXXXXXXXXXXXXXXXXXXXX 317
           AYVAKELY MGCSEISLGDTIGVGTPGTVIPMLESVVDV                     
Sbjct: 194 AYVAKELYSMGCSEISLGDTIGVGTPGTVIPMLESVVDVVPIENLAVHFHDTYGQALSNI 253

Query: 318 XXXXXXXXXXVDCSVSGLGGCPYAKGASGNVATEDVVYMLNGIGVKTNVDLEKLVLAGDF 377
                     VDCSVSGLGGCPYAKGASGNVATEDVVYMLNGIGVKTNVDLEKL+LAGDF
Sbjct: 254 LLSLQMGIGTVDCSVSGLGGCPYAKGASGNVATEDVVYMLNGIGVKTNVDLEKLMLAGDF 313

Query: 378 ISKHLGRPSASKVAIALQKVASKPNKCTATASKL 412
           ISKHLGRPSASKVAIALQKVASKPNKCTATASKL
Sbjct: 314 ISKHLGRPSASKVAIALQKVASKPNKCTATASKL 347

BLAST of Carg09818 vs. TAIR10
Match: AT2G26800.2 (Aldolase superfamily protein)

HSP 1 Score: 416.0 bits (1068), Expect = 2.7e-116
Identity = 217/311 (69.77%), Postives = 247/311 (79.42%), Query Frame = 0

Query: 101 IPQFVKIVEVGPRDGLQNEKHIVPTAVKVELIKMLVSSGLRVVEATSFVSPKWVPQLADA 160
           IP+FVKIVEVGPRDGLQNEK+IVPT+VKVELI+ LVSSGL VVEATSFVSPKWVPQLADA
Sbjct: 164 IPKFVKIVEVGPRDGLQNEKNIVPTSVKVELIQRLVSSGLPVVEATSFVSPKWVPQLADA 223

Query: 161 NDVMEGIRNVEGARFPVLTPNIKGFEAAVAAGAKEVAIFASASESFSKSNINCSIEESLR 220
            DVM+ +  ++GAR PVLTPN+KGF+AAV+AGAKEVAIFASASESFS SNINC+IEESL 
Sbjct: 224 KDVMDAVNTLDGARLPVLTPNLKGFQAAVSAGAKEVAIFASASESFSLSNINCTIEESLL 283

Query: 221 RYREVAVAARKHSLPVRGYVSCVVGCPIEGMVPPSNVAYVAKELYGMGCSEISLGDTIGV 280
           RYR VA AA++HS+PVRGYVSCVVGCP+EG V PS VAYV KELY MGC EISLGDTIG+
Sbjct: 284 RYRVVATAAKEHSVPVRGYVSCVVGCPVEGPVLPSKVAYVVKELYDMGCFEISLGDTIGI 343

Query: 281 GTPGTVIPMLESVVDVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVDCSVSGLGGCPY 340
           GTPG+V+PMLE+V+ V                               VD S++GLGGCPY
Sbjct: 344 GTPGSVVPMLEAVMAVVPADKLAVHFHDTYGQALANILVSLQMGISIVDSSIAGLGGCPY 403

Query: 341 AKGASGNVATEDVVYMLNGIGVKTNVDLEKLVLAGDFISKHLGRPSASKVAIALQKVASK 400
           AKGASGNVATEDVVYMLNG+GV TNVDL KL+ AGDFISKHLGRP+ SK A+AL +    
Sbjct: 404 AKGASGNVATEDVVYMLNGLGVHTNVDLGKLIAAGDFISKHLGRPNGSKAAVALNR---- 463

Query: 401 PNKCTATASKL 412
             + TA ASK+
Sbjct: 464 --RITADASKI 468

BLAST of Carg09818 vs. Swiss-Prot
Match: sp|O81027|HMGCL_ARATH (Hydroxymethylglutaryl-CoA lyase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=HMGCL PE=1 SV=2)

HSP 1 Score: 416.0 bits (1068), Expect = 4.9e-115
Identity = 217/311 (69.77%), Postives = 247/311 (79.42%), Query Frame = 0

Query: 101 IPQFVKIVEVGPRDGLQNEKHIVPTAVKVELIKMLVSSGLRVVEATSFVSPKWVPQLADA 160
           IP+FVKIVEVGPRDGLQNEK+IVPT+VKVELI+ LVSSGL VVEATSFVSPKWVPQLADA
Sbjct: 164 IPKFVKIVEVGPRDGLQNEKNIVPTSVKVELIQRLVSSGLPVVEATSFVSPKWVPQLADA 223

Query: 161 NDVMEGIRNVEGARFPVLTPNIKGFEAAVAAGAKEVAIFASASESFSKSNINCSIEESLR 220
            DVM+ +  ++GAR PVLTPN+KGF+AAV+AGAKEVAIFASASESFS SNINC+IEESL 
Sbjct: 224 KDVMDAVNTLDGARLPVLTPNLKGFQAAVSAGAKEVAIFASASESFSLSNINCTIEESLL 283

Query: 221 RYREVAVAARKHSLPVRGYVSCVVGCPIEGMVPPSNVAYVAKELYGMGCSEISLGDTIGV 280
           RYR VA AA++HS+PVRGYVSCVVGCP+EG V PS VAYV KELY MGC EISLGDTIG+
Sbjct: 284 RYRVVATAAKEHSVPVRGYVSCVVGCPVEGPVLPSKVAYVVKELYDMGCFEISLGDTIGI 343

Query: 281 GTPGTVIPMLESVVDVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVDCSVSGLGGCPY 340
           GTPG+V+PMLE+V+ V                               VD S++GLGGCPY
Sbjct: 344 GTPGSVVPMLEAVMAVVPADKLAVHFHDTYGQALANILVSLQMGISIVDSSIAGLGGCPY 403

Query: 341 AKGASGNVATEDVVYMLNGIGVKTNVDLEKLVLAGDFISKHLGRPSASKVAIALQKVASK 400
           AKGASGNVATEDVVYMLNG+GV TNVDL KL+ AGDFISKHLGRP+ SK A+AL +    
Sbjct: 404 AKGASGNVATEDVVYMLNGLGVHTNVDLGKLIAAGDFISKHLGRPNGSKAAVALNR---- 463

Query: 401 PNKCTATASKL 412
             + TA ASK+
Sbjct: 464 --RITADASKI 468

BLAST of Carg09818 vs. Swiss-Prot
Match: sp|Q8JZS7|HMGC2_MOUSE (3-hydroxymethyl-3-methylglutaryl-CoA lyase, cytoplasmic OS=Mus musculus OX=10090 GN=Hmgcll1 PE=2 SV=1)

HSP 1 Score: 340.1 bits (871), Expect = 3.4e-92
Identity = 176/312 (56.41%), Postives = 218/312 (69.87%), Query Frame = 0

Query: 82  GDIVLSRENKRRLSQYLRHIPQFVKIVEVGPRDGLQNEKHIVPTAVKVELIKMLVSSGLR 141
           GD V    +  + +  L  +P++VKIVEVGPRDGLQNEK IVPT +K+ELI  L  +GL 
Sbjct: 25  GDSVAGALDAAQEASQLPGLPEYVKIVEVGPRDGLQNEKVIVPTDIKIELINQLSQTGLS 84

Query: 142 VVEATSFVSPKWVPQLADANDVMEGIRNVEGARFPVLTPNIKGFEAAVAAGAKEVAIFAS 201
           V+E TSFVS +WVPQ+AD  +VM GIR   G R+PVLTPN++GF+ AVAAGA E+A+F +
Sbjct: 85  VIEVTSFVSSRWVPQMADHAEVMRGIRQYPGVRYPVLTPNLQGFQHAVAAGATEIAVFGA 144

Query: 202 ASESFSKSNINCSIEESLRRYREVAVAARKHSLPVRGYVSCVVGCPIEGMVPPSNVAYVA 261
           ASESFSK NINCSIEES+ R++EV  +AR   +PVRGYVSC +GCP EG + P  V  V+
Sbjct: 145 ASESFSKKNINCSIEESMGRFQEVISSARHMDIPVRGYVSCALGCPYEGSITPQKVTEVS 204

Query: 262 KELYGMGCSEISLGDTIGVGTPGTVIPMLESVVDVXXXXXXXXXXXXXXXXXXXXXXXXX 321
           K LYGMGC EISLGDTIGVGTPG++  MLESV+                           
Sbjct: 205 KRLYGMGCYEISLGDTIGVGTPGSMKMMLESVMKEIPPGALAVHCHDTYGQALANILTAL 264

Query: 322 XXXXXXVDCSVSGLGGCPYAKGASGNVATEDVVYMLNGIGVKTNVDLEKLVLAGDFISKH 381
                 VD +VSGLGGCPYAKGASGNVATED++YMLNG+G+ T VDL K++ AG+FI K 
Sbjct: 265 QMGINVVDSAVSGLGGCPYAKGASGNVATEDLIYMLNGMGLNTGVDLYKVMEAGEFICKA 324

Query: 382 LGRPSASKVAIA 394
           + + + SKVA A
Sbjct: 325 VNKTTNSKVAQA 336

BLAST of Carg09818 vs. Swiss-Prot
Match: sp|P97519|HMGCL_RAT (Hydroxymethylglutaryl-CoA lyase, mitochondrial OS=Rattus norvegicus OX=10116 GN=Hmgcl PE=2 SV=1)

HSP 1 Score: 335.5 bits (859), Expect = 8.3e-91
Identity = 176/297 (59.26%), Postives = 215/297 (72.39%), Query Frame = 0

Query: 101 IPQFVKIVEVGPRDGLQNEKHIVPTAVKVELIKMLVSSGLRVVEATSFVSPKWVPQLADA 160
           +P+ VKIVEVGPRDGLQNEK IVPT VK++LI ML  +GL V+EATSFVSPKWVPQ+AD 
Sbjct: 29  LPKRVKIVEVGPRDGLQNEKSIVPTPVKIKLIDMLSEAGLPVIEATSFVSPKWVPQMADH 88

Query: 161 NDVMEGIRNVEGARFPVLTPNIKGFEAAVAAGAKEVAIFASASESFSKSNINCSIEESLR 220
           +DV++GI+   G  +PVLTPN+KGFE AVAAGAKEV+IF +ASE F++ N+NCSIEES +
Sbjct: 89  SDVLKGIQKFPGINYPVLTPNMKGFEEAVAAGAKEVSIFGAASELFTRKNVNCSIEESFQ 148

Query: 221 RYREVAVAARKHSLPVRGYVSCVVGCPIEGMVPPSNVAYVAKELYGMGCSEISLGDTIGV 280
           R+  V  AAR  S+ VRGYVSC +GCP EG V P+ VA VAK+LY MGC EISLGDTIGV
Sbjct: 149 RFDGVMQAARAASISVRGYVSCALGCPYEGKVSPAKVAEVAKKLYSMGCYEISLGDTIGV 208

Query: 281 GTPGTVIPMLESVVDVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVDCSVSGLGGCPY 340
           GTPG +  ML +V+                                 VD SV+GLGGCPY
Sbjct: 209 GTPGLMKDMLTAVLHEVPVAALAVHCHDTYGQALANTLVALQMGVSVVDSSVAGLGGCPY 268

Query: 341 AKGASGNVATEDVVYMLNGIGVKTNVDLEKLVLAGDFISKHLGRPSASKVAIALQKV 398
           AKGASGN+ATED+VYML G+G+ T V+L+KL+ AGDFI + L R ++SKVA A  K+
Sbjct: 269 AKGASGNLATEDLVYMLTGLGIHTGVNLQKLLEAGDFICQALNRKTSSKVAQATCKL 325

BLAST of Carg09818 vs. Swiss-Prot
Match: sp|P38060|HMGCL_MOUSE (Hydroxymethylglutaryl-CoA lyase, mitochondrial OS=Mus musculus OX=10090 GN=Hmgcl PE=1 SV=2)

HSP 1 Score: 332.8 bits (852), Expect = 5.4e-90
Identity = 174/297 (58.59%), Postives = 213/297 (71.72%), Query Frame = 0

Query: 101 IPQFVKIVEVGPRDGLQNEKHIVPTAVKVELIKMLVSSGLRVVEATSFVSPKWVPQLADA 160
           +P+ VKIVEVGPRDGLQNEK IVPT VK+ LI ML  +GL V+EATSFVSPKWVPQ+AD 
Sbjct: 29  LPKQVKIVEVGPRDGLQNEKSIVPTPVKIRLIDMLSEAGLPVIEATSFVSPKWVPQMADH 88

Query: 161 NDVMEGIRNVEGARFPVLTPNIKGFEAAVAAGAKEVAIFASASESFSKSNINCSIEESLR 220
           +DV++GI+   G  +PVLTPN+KGFE AVAAGAKEV++F + SE F++ N NCSIEES +
Sbjct: 89  SDVLKGIQKFPGINYPVLTPNMKGFEEAVAAGAKEVSVFGAVSELFTRKNANCSIEESFQ 148

Query: 221 RYREVAVAARKHSLPVRGYVSCVVGCPIEGMVPPSNVAYVAKELYGMGCSEISLGDTIGV 280
           R+  V  AA+  S+ VRGYVSC +GCP EG V P+ VA VAK+LY MGC EISLGDTIGV
Sbjct: 149 RFAGVMQAAQAASISVRGYVSCALGCPYEGKVSPAKVAEVAKKLYSMGCYEISLGDTIGV 208

Query: 281 GTPGTVIPMLESVVDVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVDCSVSGLGGCPY 340
           GTPG +  ML +V+                                 VD SV+GLGGCPY
Sbjct: 209 GTPGLMKDMLTAVMHEVPVTALAVHCHDTYGQALANTLVALQMGVSVVDSSVAGLGGCPY 268

Query: 341 AKGASGNVATEDVVYMLNGIGVKTNVDLEKLVLAGDFISKHLGRPSASKVAIALQKV 398
           AKGASGN+ATED+VYMLNG+G+ T V+L+KL+ AGDFI + L R ++SKVA A  K+
Sbjct: 269 AKGASGNLATEDLVYMLNGLGIHTGVNLQKLLEAGDFICQALNRKTSSKVAQATCKL 325

BLAST of Carg09818 vs. Swiss-Prot
Match: sp|P35915|HMGCL_CHICK (Hydroxymethylglutaryl-CoA lyase, mitochondrial OS=Gallus gallus OX=9031 GN=HMGCL PE=1 SV=1)

HSP 1 Score: 332.4 bits (851), Expect = 7.1e-90
Identity = 172/292 (58.90%), Postives = 210/292 (71.92%), Query Frame = 0

Query: 102 PQFVKIVEVGPRDGLQNEKHIVPTAVKVELIKMLVSSGLRVVEATSFVSPKWVPQLADAN 161
           PQ VK+VEVGPRDGLQNEK +VPT VK+ LI ML  +GL V+EATSFVSP+WVPQ+AD  
Sbjct: 3   PQRVKVVEVGPRDGLQNEKSVVPTPVKIRLIDMLSETGLPVIEATSFVSPRWVPQMADHA 62

Query: 162 DVMEGIRNVEGARFPVLTPNIKGFEAAVAAGAKEVAIFASASESFSKSNINCSIEESLRR 221
           +VM+GI  + G  +PVLTPN+KGF+AAVAAGAKEV+IF +ASE F+K NINCSIEESL R
Sbjct: 63  EVMQGINKLPGVSYPVLTPNLKGFQAAVAAGAKEVSIFGAASELFTKKNINCSIEESLER 122

Query: 222 YREVAVAARKHSLPVRGYVSCVVGCPIEGMVPPSNVAYVAKELYGMGCSEISLGDTIGVG 281
           + EV  AAR  S+PVRGYVSCV+GCP EG +  + VA V+K++Y MGC EISLGD IG+G
Sbjct: 123 FSEVMNAARAASIPVRGYVSCVLGCPYEGNISAAKVAEVSKKMYSMGCYEISLGDRIGIG 182

Query: 282 TPGTVIPMLESVVDVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVDCSVSGLGGCPYA 341
           TPG++  ML +V+                                 VD SV+GLGGCPYA
Sbjct: 183 TPGSMKEMLAAVMKEVPVGALAVHCHDTYGQALANILVALQMGVSVVDASVAGLGGCPYA 242

Query: 342 KGASGNVATEDVVYMLNGIGVKTNVDLEKLVLAGDFISKHLGRPSASKVAIA 394
           +GASGNVATED+VYMLNG+G+ T VDL+KL+  G FI   L R + SKV+ A
Sbjct: 243 QGASGNVATEDLVYMLNGLGIHTGVDLQKLMDTGTFICNALNRRTNSKVSQA 294

BLAST of Carg09818 vs. TrEMBL
Match: tr|A0A0A0LHM6|A0A0A0LHM6_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G823050 PE=4 SV=1)

HSP 1 Score: 570.9 bits (1470), Expect = 2.4e-159
Identity = 308/392 (78.57%), Postives = 320/392 (81.63%), Query Frame = 0

Query: 20  MQAAKVSAKLTRRGLSWRHGYNPTMRISSRFLSSSSASHVGVSSTNGLGIQGEVDNCCQQ 79
           MQAAK+SAK TR+  SWRH YN +M   + FL S SA+ VGVSS    GIQ E  NC QQ
Sbjct: 1   MQAAKLSAKFTRQSFSWRHAYNLSMETPTPFLVSPSATDVGVSSIRTPGIQREFYNCSQQ 60

Query: 80  DGGDIVLSRENKRRLSQYLRHIPQFVKIVEVGPRDGLQNEKHIVPTAVKVELIKMLVSSG 139
           DG D VLSRENK  LSQYL  IP FVKIVEVGPRDGLQNEKHIVPTAVKV+LIKMLVSSG
Sbjct: 61  DGRDAVLSRENKGGLSQYLCRIPPFVKIVEVGPRDGLQNEKHIVPTAVKVDLIKMLVSSG 120

Query: 140 LRVVEATSFVSPKWVPQLADANDVMEGIRNVEGARFPVLTPNIKGFEAAVAAGAKEVAIF 199
           L VVEATSFVSPKWVPQLADA +VMEGIRNVEGARFPVLTPN+KGFEAAVAAGAKEVAIF
Sbjct: 121 LPVVEATSFVSPKWVPQLADAKEVMEGIRNVEGARFPVLTPNLKGFEAAVAAGAKEVAIF 180

Query: 200 ASASESFSKSNINCSIEESLRRYREVAVAARKHSLPVRGYVSCVVGCPIEGMVPPSNVAY 259
           ASASESFSKSNINCSIEESL RYR+VA AARKHSLPVRGYVSCVVGCPIEGMVP SNVAY
Sbjct: 181 ASASESFSKSNINCSIEESLHRYRQVADAARKHSLPVRGYVSCVVGCPIEGMVPASNVAY 240

Query: 260 VAKELYGMGCSEISLGDTIGVGTPGTVIPMLESVVDVXXXXXXXXXXXXXXXXXXXXXXX 319
           VAKELY MGCSEISLGDTIGVGTPGTVIPMLESV +V                       
Sbjct: 241 VAKELYSMGCSEISLGDTIGVGTPGTVIPMLESVANVVPTENLAVHFHDTYGQALSNILV 300

Query: 320 XXXXXXXXVDCSVSGLGGCPYAKGASGNVATEDVVYMLNGIGVKTNVDLEKLVLAGDFIS 379
                   VD SVSGLGGCPYAKGASGNVATEDVVYMLNGIG KTNVDL KL+LAGDFI 
Sbjct: 301 SLQMGIGTVDSSVSGLGGCPYAKGASGNVATEDVVYMLNGIGAKTNVDLGKLMLAGDFIC 360

Query: 380 KHLGRPSASKVAIALQKVASKPNKCTATASKL 412
           K LGRPSASKVAIAL+KVAS  NKCTATASKL
Sbjct: 361 KQLGRPSASKVAIALRKVASNTNKCTATASKL 392

BLAST of Carg09818 vs. TrEMBL
Match: tr|A0A1S3B7B2|A0A1S3B7B2_CUCME (hydroxymethylglutaryl-CoA lyase, mitochondrial-like OS=Cucumis melo OX=3656 GN=LOC103486948 PE=4 SV=1)

HSP 1 Score: 564.7 bits (1454), Expect = 1.7e-157
Identity = 306/392 (78.06%), Postives = 319/392 (81.38%), Query Frame = 0

Query: 20  MQAAKVSAKLTRRGLSWRHGYNPTMRISSRFLSSSSASHVGVSSTNGLGIQGEVDNCCQQ 79
           MQAAK+SAK TR+  SWRH YN    +S + L S SA+ VGV+S    GIQ E  NC QQ
Sbjct: 1   MQAAKLSAKFTRQAFSWRHAYN----LSMKTLVSPSAADVGVNSITTPGIQREFYNCSQQ 60

Query: 80  DGGDIVLSRENKRRLSQYLRHIPQFVKIVEVGPRDGLQNEKHIVPTAVKVELIKMLVSSG 139
           DG D VLSRENK  LSQ L  IP FVKIVEVGPRDGLQNEKHIVPTAVKV+LIKMLV SG
Sbjct: 61  DGRDAVLSRENKGGLSQNLCRIPPFVKIVEVGPRDGLQNEKHIVPTAVKVDLIKMLVFSG 120

Query: 140 LRVVEATSFVSPKWVPQLADANDVMEGIRNVEGARFPVLTPNIKGFEAAVAAGAKEVAIF 199
           L VVEATSFVSPKWVPQLADA DVMEGIRNVEGARFPVLTPN+KGFEAAVAAGAKEVAIF
Sbjct: 121 LPVVEATSFVSPKWVPQLADAKDVMEGIRNVEGARFPVLTPNLKGFEAAVAAGAKEVAIF 180

Query: 200 ASASESFSKSNINCSIEESLRRYREVAVAARKHSLPVRGYVSCVVGCPIEGMVPPSNVAY 259
           ASASESFSKSNINCSIEESL RYR+VA AARKHSLPVRGYVSCVVGCPIEGMVP SNVAY
Sbjct: 181 ASASESFSKSNINCSIEESLHRYRQVADAARKHSLPVRGYVSCVVGCPIEGMVPASNVAY 240

Query: 260 VAKELYGMGCSEISLGDTIGVGTPGTVIPMLESVVDVXXXXXXXXXXXXXXXXXXXXXXX 319
           VAKELY MGCSEISLGDTIGVGTPGTVIPMLESV++V                       
Sbjct: 241 VAKELYSMGCSEISLGDTIGVGTPGTVIPMLESVINVVPIENLAVHFHDTYGQALSNILV 300

Query: 320 XXXXXXXXVDCSVSGLGGCPYAKGASGNVATEDVVYMLNGIGVKTNVDLEKLVLAGDFIS 379
                   VD SVSGLGGCPYAKGASGNVATEDVVYMLNGIG KTNVDLEKL+LAGDFI 
Sbjct: 301 SLQMGIGTVDSSVSGLGGCPYAKGASGNVATEDVVYMLNGIGAKTNVDLEKLMLAGDFIC 360

Query: 380 KHLGRPSASKVAIALQKVASKPNKCTATASKL 412
           KHLGRPSASKVAIAL+KV S  NKCTATASKL
Sbjct: 361 KHLGRPSASKVAIALRKVTSNANKCTATASKL 388

BLAST of Carg09818 vs. TrEMBL
Match: tr|W9SJS1|W9SJS1_9ROSA (Hydroxymethylglutaryl-CoA lyase OS=Morus notabilis OX=981085 GN=L484_014678 PE=4 SV=1)

HSP 1 Score: 447.2 bits (1149), Expect = 4.0e-122
Identity = 241/365 (66.03%), Postives = 276/365 (75.62%), Query Frame = 0

Query: 57  SHVGVSSTNGLGIQGE----------VDNCCQQDGGDIVLSRENKRRLSQYLRHIPQFVK 116
           S V ++   GLG  G             NC ++        +++ +  ++ + +IP++VK
Sbjct: 2   SQVDITRNGGLGSLGRNYRILGNGDGAGNCFRRHYSSQFDGKQSGKSTNKIVGNIPEYVK 61

Query: 117 IVEVGPRDGLQNEKHIVPTAVKVELIKMLVSSGLRVVEATSFVSPKWVPQLADANDVMEG 176
           IVEVGPRDGLQNEKHI+PTA+KVELIK LVSSGLRV+EA+SFVSPKWVPQLADA DVME 
Sbjct: 62  IVEVGPRDGLQNEKHIIPTAIKVELIKKLVSSGLRVIEASSFVSPKWVPQLADAKDVMEA 121

Query: 177 IRNVEGARFPVLTPNIKGFEAAVAAGAKEVAIFASASESFSKSNINCSIEESLRRYREVA 236
           IR+VEGARFPVLTPN+KGFEAAVAAGAKEVAIFASASESFSKSNINCSIE+SL RYR+VA
Sbjct: 122 IRDVEGARFPVLTPNLKGFEAAVAAGAKEVAIFASASESFSKSNINCSIEDSLIRYRDVA 181

Query: 237 VAARKHSLPVRGYVSCVVGCPIEGMVPPSNVAYVAKELYGMGCSEISLGDTIGVGTPGTV 296
           +AA+K ++PVRGY+SCVVGCP+EG VPPS VA+VAKELY MGCSEISLGDTIGVGTPGTV
Sbjct: 182 LAAKKLTIPVRGYISCVVGCPVEGKVPPSQVAHVAKELYAMGCSEISLGDTIGVGTPGTV 241

Query: 297 IPMLESVVDVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVDCSVSGLGGCPYAKGASG 356
           IPMLE+V D                                VD SVSGLGGCPYAKGASG
Sbjct: 242 IPMLEAVTDAVPIKKLAVHFHDTYGQALSNMLVSLQVGITTVDSSVSGLGGCPYAKGASG 301

Query: 357 NVATEDVVYMLNGIGVKTNVDLEKLVLAGDFISKHLGRPSASKVAIALQKVASKPNKCTA 412
           NVA+EDVVYMLNG+GVKTNVDL KL+LAG+FIS HLGR S SKVAIAL+K     N C  
Sbjct: 302 NVASEDVVYMLNGLGVKTNVDLGKLILAGEFISNHLGRSSGSKVAIALRK-----NTC-- 358

BLAST of Carg09818 vs. TrEMBL
Match: tr|A0A251N621|A0A251N621_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_7G031100 PE=4 SV=1)

HSP 1 Score: 444.5 bits (1142), Expect = 2.6e-121
Identity = 235/321 (73.21%), Postives = 258/321 (80.37%), Query Frame = 0

Query: 91  KRRLSQYLRHIPQFVKIVEVGPRDGLQNEKHIVPTAVKVELIKMLVSSGLRVVEATSFVS 150
           K  +S  L++IP+FVKIVEVGPRDGLQNEKHIVPTAVKV+LI+MLV+SGL VVEATSFVS
Sbjct: 57  KEPISNLLKNIPEFVKIVEVGPRDGLQNEKHIVPTAVKVKLIEMLVASGLDVVEATSFVS 116

Query: 151 PKWVPQLADANDVMEGIRNVEGARFPVLTPNIKGFEAAVAAGAKEVAIFASASESFSKSN 210
           PKWVPQLADA DVM+ IRNVEG RFPVLTPN+KGFEAAVAAGAKEVAIFASASESFSKSN
Sbjct: 117 PKWVPQLADAKDVMKAIRNVEGVRFPVLTPNLKGFEAAVAAGAKEVAIFASASESFSKSN 176

Query: 211 INCSIEESLRRYREVAVAARKHSLPVRGYVSCVVGCPIEGMVPPSNVAYVAKELYGMGCS 270
           INCSIE+SL RY +VA AA K S+PVRGY+SCVVGCP+EG V PS VAYVAK+LY MGCS
Sbjct: 177 INCSIEDSLIRYHDVATAATKLSIPVRGYISCVVGCPVEGTVLPSQVAYVAKKLYNMGCS 236

Query: 271 EISLGDTIGVGTPGTVIPMLESVVDVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVDC 330
           +ISLGDTIGVGTPGT+IPMLE+V DV                               VD 
Sbjct: 237 DISLGDTIGVGTPGTIIPMLEAVTDVVPTDKLAVHYHDTYGQALSNILASLQMGISTVDS 296

Query: 331 SVSGLGGCPYAKGASGNVATEDVVYMLNGIGVKTNVDLEKLVLAGDFISKHLGRPSASKV 390
           SVSGLGGCPYAKGASGNVATEDVVYMLNG+GVKTNVDL+KL+LAGDFI KHLGR S SK 
Sbjct: 297 SVSGLGGCPYAKGASGNVATEDVVYMLNGLGVKTNVDLKKLMLAGDFICKHLGRSSGSKT 356

Query: 391 AIALQKVASKPNKCTATASKL 412
           A+A+ KV       TA ASKL
Sbjct: 357 AVAMSKV-------TAHASKL 370

BLAST of Carg09818 vs. TrEMBL
Match: tr|A0A2P4IAJ8|A0A2P4IAJ8_QUESU (Isoform 2 of hydroxymethylglutaryl-coa lyase, mitochondrial OS=Quercus suber OX=58331 GN=CFP56_47127 PE=4 SV=1)

HSP 1 Score: 444.1 bits (1141), Expect = 3.4e-121
Identity = 230/309 (74.43%), Postives = 254/309 (82.20%), Query Frame = 0

Query: 95  SQYLRHIPQFVKIVEVGPRDGLQNEKHIVPTAVKVELIKMLVSSGLRVVEATSFVSPKWV 154
           +++L+ IP FVKIVEVGPRDGLQNE +IVPTAVK+ELIK LVSSGL VVEATSFVSPKWV
Sbjct: 105 NKFLKSIPGFVKIVEVGPRDGLQNESNIVPTAVKIELIKRLVSSGLPVVEATSFVSPKWV 164

Query: 155 PQLADANDVMEGIRNVEGARFPVLTPNIKGFEAAVAAGAKEVAIFASASESFSKSNINCS 214
           PQLADA DVME IRNVE ARFPVLTPN+KGFEAAVAAGAKEVAIFASASE+FSKSNINCS
Sbjct: 165 PQLADAKDVMEAIRNVEAARFPVLTPNLKGFEAAVAAGAKEVAIFASASEAFSKSNINCS 224

Query: 215 IEESLRRYREVAVAARKHSLPVRGYVSCVVGCPIEGMVPPSNVAYVAKELYGMGCSEISL 274
           I++SL RY +V  AARK S+PVRGY+SCVVGCP+EG+VPP+ VAYVAKELY MGCSEISL
Sbjct: 225 IKDSLTRYSDVTFAARKVSIPVRGYISCVVGCPVEGVVPPAKVAYVAKELYDMGCSEISL 284

Query: 275 GDTIGVGTPGTVIPMLESVVDVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVDCSVSG 334
           GDTIGVGTPGTVIPMLE+V+DV                               VD SVSG
Sbjct: 285 GDTIGVGTPGTVIPMLEAVMDVVPVDRLAVHFHDTYGQALSNILVSLQMGISTVDSSVSG 344

Query: 335 LGGCPYAKGASGNVATEDVVYMLNGIGVKTNVDLEKLVLAGDFISKHLGRPSASKVAIAL 394
           LGGCPYAKGASGNVATEDVVYML+G+GVKTNV+L KL+LAGDFISKHLGRPS SK A+AL
Sbjct: 345 LGGCPYAKGASGNVATEDVVYMLHGLGVKTNVNLGKLMLAGDFISKHLGRPSGSKTAVAL 404

Query: 395 QKVASKPNK 404
            K  S  +K
Sbjct: 405 SKATSNASK 413

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022934490.19.1e-19599.49hydroxymethylglutaryl-CoA lyase, mitochondrial-like isoform X1 [Cucurbita moscha... [more]
XP_023526450.13.8e-19390.82hydroxymethylglutaryl-CoA lyase, mitochondrial-like isoform X1 [Cucurbita pepo s... [more]
XP_022983793.19.4e-19290.56hydroxymethylglutaryl-CoA lyase, mitochondrial-like isoform X1 [Cucurbita maxima... [more]
XP_022934491.11.5e-16099.40hydroxymethylglutaryl-CoA lyase, mitochondrial-like isoform X2 [Cucurbita moscha... [more]
XP_023526452.12.8e-15989.52hydroxymethylglutaryl-CoA lyase, mitochondrial-like isoform X2 [Cucurbita pepo s... [more]
Match NameE-valueIdentityDescription
AT2G26800.22.7e-11669.77Aldolase superfamily protein[more]
Match NameE-valueIdentityDescription
sp|O81027|HMGCL_ARATH4.9e-11569.77Hydroxymethylglutaryl-CoA lyase, mitochondrial OS=Arabidopsis thaliana OX=3702 G... [more]
sp|Q8JZS7|HMGC2_MOUSE3.4e-9256.413-hydroxymethyl-3-methylglutaryl-CoA lyase, cytoplasmic OS=Mus musculus OX=10090... [more]
sp|P97519|HMGCL_RAT8.3e-9159.26Hydroxymethylglutaryl-CoA lyase, mitochondrial OS=Rattus norvegicus OX=10116 GN=... [more]
sp|P38060|HMGCL_MOUSE5.4e-9058.59Hydroxymethylglutaryl-CoA lyase, mitochondrial OS=Mus musculus OX=10090 GN=Hmgcl... [more]
sp|P35915|HMGCL_CHICK7.1e-9058.90Hydroxymethylglutaryl-CoA lyase, mitochondrial OS=Gallus gallus OX=9031 GN=HMGCL... [more]
Match NameE-valueIdentityDescription
tr|A0A0A0LHM6|A0A0A0LHM6_CUCSA2.4e-15978.57Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G823050 PE=4 SV=1[more]
tr|A0A1S3B7B2|A0A1S3B7B2_CUCME1.7e-15778.06hydroxymethylglutaryl-CoA lyase, mitochondrial-like OS=Cucumis melo OX=3656 GN=L... [more]
tr|W9SJS1|W9SJS1_9ROSA4.0e-12266.03Hydroxymethylglutaryl-CoA lyase OS=Morus notabilis OX=981085 GN=L484_014678 PE=4... [more]
tr|A0A251N621|A0A251N621_PRUPE2.6e-12173.21Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_7G031100 PE=4 SV=1[more]
tr|A0A2P4IAJ8|A0A2P4IAJ8_QUESU3.4e-12174.43Isoform 2 of hydroxymethylglutaryl-coa lyase, mitochondrial OS=Quercus suber OX=... [more]
The following terms have been associated with this gene:
Vocabulary: Cellular Component
TermDefinition
GO:0005739mitochondrion
Vocabulary: Molecular Function
TermDefinition
GO:0004419hydroxymethylglutaryl-CoA lyase activity
GO:0003824catalytic activity
Vocabulary: Biological Process
TermDefinition
GO:0046951ketone body biosynthetic process
Vocabulary: INTERPRO
TermDefinition
IPR030020HL_mitochondrial
IPR000891PYR_CT
IPR013785Aldolase_TIM
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006550 isoleucine catabolic process
biological_process GO:0046951 ketone body biosynthetic process
biological_process GO:0006552 leucine catabolic process
biological_process GO:0006574 valine catabolic process
cellular_component GO:0005739 mitochondrion
molecular_function GO:0004419 hydroxymethylglutaryl-CoA lyase activity
molecular_function GO:0003824 catalytic activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg09818-RACarg09818-RAmRNA


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013785Aldolase-type TIM barrelGENE3DG3DSA:3.20.20.70coord: 98..398
e-value: 4.0E-131
score: 438.8
IPR000891Pyruvate carboxyltransferasePFAMPF00682HMGL-likecoord: 105..377
e-value: 1.3E-55
score: 188.8
IPR000891Pyruvate carboxyltransferasePROSITEPS50991PYR_CTcoord: 105..372
score: 34.961
NoneNo IPR availablePANTHERPTHR42738FAMILY NOT NAMEDcoord: 89..400
NoneNo IPR availableCDDcd07938DRE_TIM_HMGLcoord: 107..380
e-value: 3.5207E-173
score: 487.285
NoneNo IPR availableSUPERFAMILYSSF51569Aldolasecoord: 100..394
IPR030020Hydroxymethylglutaryl-CoA lyase, mitochondrialPANTHERPTHR42738:SF6SUBFAMILY NOT NAMEDcoord: 89..400