Cp4.1LG01g00800 (gene) Cucurbita pepo (Zucchini)

NameCp4.1LG01g00800
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
Description9-cis-epoxycarotenoid dioxygenase
LocationCp4.1LG01 : 3360292 .. 3362052 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCTTCTTCTTCTTCTTCTTCAATGCCGAAGATTCTCTCTGTAAATAGGAATTATGGTGTAAGAAACAGAGTGTCTTGTTCGCTTCATACTCCGTCTATAATTCAAATTCCTAAGCGGTCTCAGACGGCATTTCGGTCGCCCATCAAGATCGCCGTGCCAAAACCTGAACCTGTTGCTGCTCCCTCCGTGTCTTCGCCCGCCAAGGAGTGGAATTTCTTGCAGCAAGCTGCGGCTATGGCGCTCGATGCGGTTGAGGATGCTCTGGTTTCGATGGAGAAGAAGCATTCCTTGCCTAAGACGGCTGACCCTTCTGTTCAAATTGCTGGGAATTTTGCTCCTGTGCCGGAGCAGTCTGTTCGGAAGGTGTTACCGGTCACCGGGAAAGTTCCCGACTGCATTCGGGGGGTTTATGTGCGAAACGGAGCCAACCCACTTCATGAACCGGTTTCCGGTCATCACTTGTTTGACGGCGATGGGATGGTCCATGCTGTGGAGTTCACTGAGGGTGGTAGAGTTAGTTACGCTTGCCGGTTTACGGAGACAGAACGGCTGGTTCAGGAGCGGGCTTATGGCCGGCCGGTTTTCCCGAAAGCTATTGGGGAACTCCATGGCCACTCTGGGATAGCTCGGTTGATGCTTTTCTATGCTAGAGGATTGTTTGGGCTTGTCGATCATAGCCATGGAATCGGCGTCGCCAACGCCGGTTTGGTTTACTTTAACGGCCGGCTTCTTGCCATGTCGGAGGATGATTTGCCGTACCAAATCCGGGTCACTCCGTCTGGGGATTTGAAAACCGTTGGCCGGTTTGATTTCGACGGGCAATTGAAATCCACAATGATAGCTCACCCAAAGCTCGACCCTGTTTCCGGCGAGATGTTCGCTTTGAGCTACGACGTAATTCAGAAACCATATTTGAAATACTTCAAATTCTCGGCGGAGGGGGAGAAATCACCGGACGTTGAAATTCCCCTGCCTCAGCCGACGATGATGCACGATTTCGCAATTACGGAAAAATTTGTTGTAATTCCAGACCAGCAGGTGGTTTTCAAGTTGCCGGAGATGATCCGCGGGGGATCTCCGGTGGTCTACGACAAAGAAAAAACCTCCCGATTTGGGATTTTGGACAAAAATGCCACAGACGCCAACGCCATTAAATGGATTGAAGCTCCCGATTGCTTCTGTTTCCATCTCTGGAACGCTTGGGAAGAACCAGAGACTAACGAAATCGTCGTAATTGGATCGTGCATGACGCCACCGGACTCCATTTTCAACGAATGTGAAGAGAATTTGAAATCCGTCTTATCGGAAATTCGCCTGAATCTCTCCACCGGAAAATCAACTCGTCGGCCGATCATCGCTGAAACAGAGCAAGTGAACTTAGAAGCCGGAATGGTGAACCGAAATCAATTAGGGCGAAAAACCCAGTTCGCTTATCTTGCTCTAGCTGAACCATGGCCAAAGGTTTCTGGATTCGCGAAGGTCGATCTCTCCACCGGAGAAATCAGAAAGTATCTCTACGGCGAAGACAGGTACGGCGGCGAGCCTCTGTTTCTTCCTAGAGAAGGGGCAACGGCGGAGGACGATGGGCACATCTTAGCCTTCGTTCACGACGAGAAGGAATGGAAATCAGAGTTGCAGATCGTAAACGCCATGACTTTAGAGCTTGAAGCTACTGTAAAGCTTCCTTCTCGAGTTCCTTACGGCTTCCATGGAACTTTCATAAGCTCCAAGGATCTGCAGAAGCAGAAACGGTAA

mRNA sequence

ATGGCTTCTTCTTCTTCTTCTTCTTCAATGCCGAAGATTCTCTCTGTAAATAGGAATTATGGTGTAAGAAACAGAGTGTCTTGTTCGCTTCATACTCCGTCTATAATTCAAATTCCTAAGCGGTCTCAGACGGCATTTCGGTCGCCCATCAAGATCGCCGTGCCAAAACCTGAACCTGTTGCTGCTCCCTCCGTGTCTTCGCCCGCCAAGGAGTGGAATTTCTTGCAGCAAGCTGCGGCTATGGCGCTCGATGCGGTTGAGGATGCTCTGGTTTCGATGGAGAAGAAGCATTCCTTGCCTAAGACGGCTGACCCTTCTGTTCAAATTGCTGGGAATTTTGCTCCTGTGCCGGAGCAGTCTGTTCGGAAGGTGTTACCGGTCACCGGGAAAGTTCCCGACTGCATTCGGGGGGTTTATGTGCGAAACGGAGCCAACCCACTTCATGAACCGGTTTCCGGTCATCACTTGTTTGACGGCGATGGGATGGTCCATGCTGTGGAGTTCACTGAGGGTGGTAGAGTTAGTTACGCTTGCCGGTTTACGGAGACAGAACGGCTGGTTCAGGAGCGGGCTTATGGCCGGCCGGTTTTCCCGAAAGCTATTGGGGAACTCCATGGCCACTCTGGGATAGCTCGGTTGATGCTTTTCTATGCTAGAGGATTGTTTGGGCTTGTCGATCATAGCCATGGAATCGGCGTCGCCAACGCCGGTTTGGTTTACTTTAACGGCCGGCTTCTTGCCATGTCGGAGGATGATTTGCCGTACCAAATCCGGGTCACTCCGTCTGGGGATTTGAAAACCGTTGGCCGGTTTGATTTCGACGGGCAATTGAAATCCACAATGATAGCTCACCCAAAGCTCGACCCTGTTTCCGGCGAGATGTTCGCTTTGAGCTACGACGTAATTCAGAAACCATATTTGAAATACTTCAAATTCTCGGCGGAGGGGGAGAAATCACCGGACGTTGAAATTCCCCTGCCTCAGCCGACGATGATGCACGATTTCGCAATTACGGAAAAATTTGTTGTAATTCCAGACCAGCAGGTGGTTTTCAAGTTGCCGGAGATGATCCGCGGGGGATCTCCGGTGGTCTACGACAAAGAAAAAACCTCCCGATTTGGGATTTTGGACAAAAATGCCACAGACGCCAACGCCATTAAATGGATTGAAGCTCCCGATTGCTTCTGTTTCCATCTCTGGAACGCTTGGGAAGAACCAGAGACTAACGAAATCGTCGTAATTGGATCGTGCATGACGCCACCGGACTCCATTTTCAACGAATGTGAAGAGAATTTGAAATCCGTCTTATCGGAAATTCGCCTGAATCTCTCCACCGGAAAATCAACTCGTCGGCCGATCATCGCTGAAACAGAGCAAGTGAACTTAGAAGCCGGAATGGTGAACCGAAATCAATTAGGGCGAAAAACCCAGTTCGCTTATCTTGCTCTAGCTGAACCATGGCCAAAGGTTTCTGGATTCGCGAAGGTCGATCTCTCCACCGGAGAAATCAGAAAGTATCTCTACGGCGAAGACAGGTACGGCGGCGAGCCTCTGTTTCTTCCTAGAGAAGGGGCAACGGCGGAGGACGATGGGCACATCTTAGCCTTCGTTCACGACGAGAAGGAATGGAAATCAGAGTTGCAGATCGTAAACGCCATGACTTTAGAGCTTGAAGCTACTGTAAAGCTTCCTTCTCGAGTTCCTTACGGCTTCCATGGAACTTTCATAAGCTCCAAGGATCTGCAGAAGCAGAAACGGTAA

Coding sequence (CDS)

ATGGCTTCTTCTTCTTCTTCTTCTTCAATGCCGAAGATTCTCTCTGTAAATAGGAATTATGGTGTAAGAAACAGAGTGTCTTGTTCGCTTCATACTCCGTCTATAATTCAAATTCCTAAGCGGTCTCAGACGGCATTTCGGTCGCCCATCAAGATCGCCGTGCCAAAACCTGAACCTGTTGCTGCTCCCTCCGTGTCTTCGCCCGCCAAGGAGTGGAATTTCTTGCAGCAAGCTGCGGCTATGGCGCTCGATGCGGTTGAGGATGCTCTGGTTTCGATGGAGAAGAAGCATTCCTTGCCTAAGACGGCTGACCCTTCTGTTCAAATTGCTGGGAATTTTGCTCCTGTGCCGGAGCAGTCTGTTCGGAAGGTGTTACCGGTCACCGGGAAAGTTCCCGACTGCATTCGGGGGGTTTATGTGCGAAACGGAGCCAACCCACTTCATGAACCGGTTTCCGGTCATCACTTGTTTGACGGCGATGGGATGGTCCATGCTGTGGAGTTCACTGAGGGTGGTAGAGTTAGTTACGCTTGCCGGTTTACGGAGACAGAACGGCTGGTTCAGGAGCGGGCTTATGGCCGGCCGGTTTTCCCGAAAGCTATTGGGGAACTCCATGGCCACTCTGGGATAGCTCGGTTGATGCTTTTCTATGCTAGAGGATTGTTTGGGCTTGTCGATCATAGCCATGGAATCGGCGTCGCCAACGCCGGTTTGGTTTACTTTAACGGCCGGCTTCTTGCCATGTCGGAGGATGATTTGCCGTACCAAATCCGGGTCACTCCGTCTGGGGATTTGAAAACCGTTGGCCGGTTTGATTTCGACGGGCAATTGAAATCCACAATGATAGCTCACCCAAAGCTCGACCCTGTTTCCGGCGAGATGTTCGCTTTGAGCTACGACGTAATTCAGAAACCATATTTGAAATACTTCAAATTCTCGGCGGAGGGGGAGAAATCACCGGACGTTGAAATTCCCCTGCCTCAGCCGACGATGATGCACGATTTCGCAATTACGGAAAAATTTGTTGTAATTCCAGACCAGCAGGTGGTTTTCAAGTTGCCGGAGATGATCCGCGGGGGATCTCCGGTGGTCTACGACAAAGAAAAAACCTCCCGATTTGGGATTTTGGACAAAAATGCCACAGACGCCAACGCCATTAAATGGATTGAAGCTCCCGATTGCTTCTGTTTCCATCTCTGGAACGCTTGGGAAGAACCAGAGACTAACGAAATCGTCGTAATTGGATCGTGCATGACGCCACCGGACTCCATTTTCAACGAATGTGAAGAGAATTTGAAATCCGTCTTATCGGAAATTCGCCTGAATCTCTCCACCGGAAAATCAACTCGTCGGCCGATCATCGCTGAAACAGAGCAAGTGAACTTAGAAGCCGGAATGGTGAACCGAAATCAATTAGGGCGAAAAACCCAGTTCGCTTATCTTGCTCTAGCTGAACCATGGCCAAAGGTTTCTGGATTCGCGAAGGTCGATCTCTCCACCGGAGAAATCAGAAAGTATCTCTACGGCGAAGACAGGTACGGCGGCGAGCCTCTGTTTCTTCCTAGAGAAGGGGCAACGGCGGAGGACGATGGGCACATCTTAGCCTTCGTTCACGACGAGAAGGAATGGAAATCAGAGTTGCAGATCGTAAACGCCATGACTTTAGAGCTTGAAGCTACTGTAAAGCTTCCTTCTCGAGTTCCTTACGGCTTCCATGGAACTTTCATAAGCTCCAAGGATCTGCAGAAGCAGAAACGGTAA

Protein sequence

MASSSSSSSMPKILSVNRNYGVRNRVSCSLHTPSIIQIPKRSQTAFRSPIKIAVPKPEPVAAPSVSSPAKEWNFLQQAAAMALDAVEDALVSMEKKHSLPKTADPSVQIAGNFAPVPEQSVRKVLPVTGKVPDCIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFTEGGRVSYACRFTETERLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHSHGIGVANAGLVYFNGRLLAMSEDDLPYQIRVTPSGDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEMFALSYDVIQKPYLKYFKFSAEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVFKLPEMIRGGSPVVYDKEKTSRFGILDKNATDANAIKWIEAPDCFCFHLWNAWEEPETNEIVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIIAETEQVNLEAGMVNRNQLGRKTQFAYLALAEPWPKVSGFAKVDLSTGEIRKYLYGEDRYGGEPLFLPREGATAEDDGHILAFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSKDLQKQKR
BLAST of Cp4.1LG01g00800 vs. Swiss-Prot
Match: NCED3_ARATH (9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic OS=Arabidopsis thaliana GN=NCED3 PE=2 SV=1)

HSP 1 Score: 877.1 bits (2265), Expect = 1.1e-253
Identity = 439/590 (74.41%), Postives = 492/590 (83.39%), Query Frame = 1

Query: 1   MASSSSS-----SSMPKILSVNRNYGVRNRVSCSLHTPSIIQIPKRSQTAFRSPIKIAVP 60
           ++SS SS     SS+P    V R   V    S +LHTP  +  PK+S     SP  +  P
Sbjct: 24  LSSSQSSDLSYCSSLPMASRVTRKLNV----SSALHTPPALHFPKQSSN---SPAIVVKP 83

Query: 61  KPEPVAAPSVSSPAKEWNFLQQAAAMALDAVEDALVSMEKKHSLPKTADPSVQIAGNFAP 120
           K +        S  K+ N  Q+AAA ALDA E  LVS EK H LPKTADPSVQIAGNFAP
Sbjct: 84  KAK-------ESNTKQMNLFQRAAAAALDAAEGFLVSHEKLHPLPKTADPSVQIAGNFAP 143

Query: 121 VPEQSVRKVLPVTGKVPDCIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFTEGGRVS 180
           V EQ VR+ LPV GK+PD I+GVYVRNGANPLHEPV+GHH FDGDGMVHAV+F E G  S
Sbjct: 144 VNEQPVRRNLPVVGKLPDSIKGVYVRNGANPLHEPVTGHHFFDGDGMVHAVKF-EHGSAS 203

Query: 181 YACRFTETERLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHSHGIGVAN 240
           YACRFT+T R VQER  GRPVFPKAIGELHGH+GIARLMLFYAR   G+VD +HG GVAN
Sbjct: 204 YACRFTQTNRFVQERQLGRPVFPKAIGELHGHTGIARLMLFYARAAAGIVDPAHGTGVAN 263

Query: 241 AGLVYFNGRLLAMSEDDLPYQIRVTPSGDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEMF 300
           AGLVYFNGRLLAMSEDDLPYQ+++TP+GDLKTVGRFDFDGQL+STMIAHPK+DP SGE+F
Sbjct: 264 AGLVYFNGRLLAMSEDDLPYQVQITPNGDLKTVGRFDFDGQLESTMIAHPKVDPESGELF 323

Query: 301 ALSYDVIQKPYLKYFKFSAEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVFKLPE 360
           ALSYDV+ KPYLKYF+FS +G KSPDVEI L QPTMMHDFAITE FVV+PDQQVVFKLPE
Sbjct: 324 ALSYDVVSKPYLKYFRFSPDGTKSPDVEIQLDQPTMMHDFAITENFVVVPDQQVVFKLPE 383

Query: 361 MIRGGSPVVYDKEKTSRFGILDKNATDANAIKWIEAPDCFCFHLWNAWEEPETNEIVVIG 420
           MIRGGSPVVYDK K +RFGILDK A D++ IKWI+APDCFCFHLWNAWEEPET+E+VVIG
Sbjct: 384 MIRGGSPVVYDKNKVARFGILDKYAEDSSNIKWIDAPDCFCFHLWNAWEEPETDEVVVIG 443

Query: 421 SCMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIIA-ETEQVNLEAGMVNRNQLGR 480
           SCMTPPDSIFNE +ENLKSVLSEIRLNL TG+STRRPII+ E +QVNLEAGMVNRN LGR
Sbjct: 444 SCMTPPDSIFNESDENLKSVLSEIRLNLKTGESTRRPIISNEDQQVNLEAGMVNRNMLGR 503

Query: 481 KTQFAYLALAEPWPKVSGFAKVDLSTGEIRKYLYGEDRYGGEPLFLPREGATAEDDGHIL 540
           KT+FAYLALAEPWPKVSGFAKVDL+TGE++K+LYG++RYGGEPLFLP EG   ED+G+IL
Sbjct: 504 KTKFAYLALAEPWPKVSGFAKVDLTTGEVKKHLYGDNRYGGEPLFLPGEGG-EEDEGYIL 563

Query: 541 AFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSKDLQKQ 585
            FVHDEK WKSELQIVNA++LE+EATVKLPSRVPYGFHGTFI + DL KQ
Sbjct: 564 CFVHDEKTWKSELQIVNAVSLEVEATVKLPSRVPYGFHGTFIGADDLAKQ 597

BLAST of Cp4.1LG01g00800 vs. Swiss-Prot
Match: NCED1_PHAVU (9-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic OS=Phaseolus vulgaris GN=NCED1 PE=2 SV=1)

HSP 1 Score: 875.9 bits (2262), Expect = 2.4e-253
Identity = 433/592 (73.14%), Postives = 489/592 (82.60%), Query Frame = 1

Query: 3   SSSSSSSMPKILSVNRNYGVRNRVSCSL---HTPSIIQIPKRSQTAFRSPIK------IA 62
           +S+SSS    +    R+    N ++CSL   H P   Q    S T   +PIK        
Sbjct: 26  ASTSSSPTTLLPFKKRSSSNTNTITCSLQTLHYPKQYQPTSTSTTTTPTPIKPTTTTTTT 85

Query: 63  VPKPEPVAAPSVSSP-AKEWNFLQQAAAMALDAVEDALVSMEKKHSLPKTADPSVQIAGN 122
            P  E         P  ++WNFLQ+AAA  LD VE ALVS E KH LPKTADP VQIAGN
Sbjct: 86  TPHRETKPLSDTKQPFPQKWNFLQKAAATGLDMVETALVSHESKHPLPKTADPKVQIAGN 145

Query: 123 FAPVPEQSVRKVLPVTGKVPDCIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFTEGG 182
           FAPVPE +  + LPV GK+P CI GVYVRNGANPL+EPV+GHH FDGDGMVHAV+FT G 
Sbjct: 146 FAPVPEHAADQALPVVGKIPKCIDGVYVRNGANPLYEPVAGHHFFDGDGMVHAVKFTNGA 205

Query: 183 RVSYACRFTETERLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHSHGIG 242
             SYACRFTET+RL QE++ GRPVFPKAIGELHGHSGIARL+LFYAR LF LVD SHG+G
Sbjct: 206 -ASYACRFTETQRLAQEKSLGRPVFPKAIGELHGHSGIARLLLFYARSLFQLVDGSHGMG 265

Query: 243 VANAGLVYFNGRLLAMSEDDLPYQIRVTPSGDLKTVGRFDFDGQLKSTMIAHPKLDPVSG 302
           VANAGLVYFN  LLAMSEDDLPY +R+T +GDL TVGR+DF+GQL STMIAHPKLDPV+G
Sbjct: 266 VANAGLVYFNNHLLAMSEDDLPYHVRITSNGDLTTVGRYDFNGQLNSTMIAHPKLDPVNG 325

Query: 303 EMFALSYDVIQKPYLKYFKFSAEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVFK 362
           ++ ALSYDV+QKPYLKYF+FSA+G KSPDVEIPL +PTMMHDFAITE FVV+PDQQVVFK
Sbjct: 326 DLHALSYDVVQKPYLKYFRFSADGVKSPDVEIPLKEPTMMHDFAITENFVVVPDQQVVFK 385

Query: 363 LPEMIRGGSPVVYDKEKTSRFGILDKNATDANAIKWIEAPDCFCFHLWNAWEEPETNEIV 422
           L EMI GGSPVVYDK KTSRFGILDKNA DANA++WI+AP+CFCFHLWNAWEEPET+EIV
Sbjct: 386 LTEMITGGSPVVYDKNKTSRFGILDKNAKDANAMRWIDAPECFCFHLWNAWEEPETDEIV 445

Query: 423 VIGSCMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIIAETEQVNLEAGMVNRNQL 482
           VIGSCMTP DSIFNEC+E+LKSVLSEIRLNL TGKSTRRPII++ EQVNLEAGMVNRN+L
Sbjct: 446 VIGSCMTPADSIFNECDESLKSVLSEIRLNLRTGKSTRRPIISDAEQVNLEAGMVNRNKL 505

Query: 483 GRKTQFAYLALAEPWPKVSGFAKVDLSTGEIRKYLYGEDRYGGEPLFLPREGATAEDDGH 542
           GRKTQFAYLALAEPWPKVSGFAKVDL +GE++KY+YGE+++GGEPLFLP      E DG+
Sbjct: 506 GRKTQFAYLALAEPWPKVSGFAKVDLFSGEVQKYMYGEEKFGGEPLFLP--NGEEEGDGY 565

Query: 543 ILAFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSKDLQKQ 585
           ILAFVHDEKEWKSELQIVNA  L+LEA++KLPSRVPYGFHGTFI SKDL+KQ
Sbjct: 566 ILAFVHDEKEWKSELQIVNAQNLKLEASIKLPSRVPYGFHGTFIHSKDLRKQ 614

BLAST of Cp4.1LG01g00800 vs. Swiss-Prot
Match: NCED9_ARATH (9-cis-epoxycarotenoid dioxygenase NCED9, chloroplastic OS=Arabidopsis thaliana GN=NCED9 PE=2 SV=1)

HSP 1 Score: 833.9 bits (2153), Expect = 1.0e-240
Identity = 417/590 (70.68%), Postives = 492/590 (83.39%), Query Frame = 1

Query: 3   SSSSSSSMPKILSVNRNYGVRNR-------VSCSLHTPSIIQIPKRSQTAFRSPIKIAVP 62
           S+SSSSS PK+ S++ +  +RN+       VS S++  S +    +S T F+ P    + 
Sbjct: 70  STSSSSSRPKLQSLSFSSTLRNKKLVVPCYVSSSVNKKSSVSSSLQSPT-FKPPSWKKLC 129

Query: 63  KPEPVAAPSVSSPAKEWNFLQQAAAMALDAVEDALVSMEKK-HSLPKTADPSVQIAGNFA 122
                  P  ++   + N +Q+ AAM LDAVE+A++S E++ H  PKTADP+VQIAGNF 
Sbjct: 130 NDVTNLIPKTTNQNPKLNPVQRTAAMVLDAVENAMISHERRRHPHPKTADPAVQIAGNFF 189

Query: 123 PVPEQSVRKVLPVTGKVPDCIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFTEGGRV 182
           PVPE+ V   LPVTG VP+CI+GVYVRNGANPLH+PVSGHHLFDGDGMVHAV F + G V
Sbjct: 190 PVPEKPVVHNLPVTGTVPECIQGVYVRNGANPLHKPVSGHHLFDGDGMVHAVRF-DNGSV 249

Query: 183 SYACRFTETERLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHSHGIGVA 242
           SYACRFTET RLVQER  GRPVFPKAIGELHGH GIA+LMLF  RGLFGLVD + G+GVA
Sbjct: 250 SYACRFTETNRLVQERECGRPVFPKAIGELHGHLGIAKLMLFNTRGLFGLVDPTGGLGVA 309

Query: 243 NAGLVYFNGRLLAMSEDDLPYQIRVTPSGDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEM 302
           NAGLVYFNG LLAMSEDDLPY ++VT +GDL+T GR+DFDGQLKSTMIAHPK+DP + E+
Sbjct: 310 NAGLVYFNGHLLAMSEDDLPYHVKVTQTGDLETSGRYDFDGQLKSTMIAHPKIDPETREL 369

Query: 303 FALSYDVIQKPYLKYFKFSAEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVFKLP 362
           FALSYDV+ KPYLKYF+F+++GEKSPDVEIPL QPTM+HDFAITE FVVIPDQQVVF+LP
Sbjct: 370 FALSYDVVSKPYLKYFRFTSDGEKSPDVEIPLDQPTMIHDFAITENFVVIPDQQVVFRLP 429

Query: 363 EMIRGGSPVVYDKEKTSRFGILDKNATDANAIKWIEAPDCFCFHLWNAWEEPETNEIVVI 422
           EMIRGGSPVVYD++K SRFGIL+KNA DA++I+WIE PDCFCFHLWN+WEEPET+E+VVI
Sbjct: 430 EMIRGGSPVVYDEKKKSRFGILNKNAKDASSIQWIEVPDCFCFHLWNSWEEPETDEVVVI 489

Query: 423 GSCMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIIAETEQVNLEAGMVNRNQLGR 482
           GSCMTPPDSIFNE +E L+SVLSEIRLNL TG+STRRP+I  +EQVNLEAGMVNRN LGR
Sbjct: 490 GSCMTPPDSIFNEHDETLQSVLSEIRLNLKTGESTRRPVI--SEQVNLEAGMVNRNLLGR 549

Query: 483 KTQFAYLALAEPWPKVSGFAKVDLSTGEIRKYLYGEDRYGGEPLFLPREGATAEDDGHIL 542
           KT++AYLAL EPWPKVSGFAKVDLSTGEIRKY+YGE +YGGEPLFLP  G   ED G+I+
Sbjct: 550 KTRYAYLALTEPWPKVSGFAKVDLSTGEIRKYIYGEGKYGGEPLFLP-SGDGEEDGGYIM 609

Query: 543 AFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSKDLQKQ 585
            FVHDE++ KSELQ++NA+ ++LEATV LPSRVPYGFHGTFIS +DL KQ
Sbjct: 610 VFVHDEEKVKSELQLINAVNMKLEATVTLPSRVPYGFHGTFISKEDLSKQ 654

BLAST of Cp4.1LG01g00800 vs. Swiss-Prot
Match: NCED5_ARATH (Probable 9-cis-epoxycarotenoid dioxygenase NCED5, chloroplastic OS=Arabidopsis thaliana GN=NCED5 PE=1 SV=1)

HSP 1 Score: 807.4 bits (2084), Expect = 1.1e-232
Identity = 388/531 (73.07%), Postives = 445/531 (83.80%), Query Frame = 1

Query: 55  PKPEPVAAPSVSSPAKEWNFLQQAAAMALDAVEDALVSMEKKHSLPKTADPSVQIAGNFA 114
           P P P+     +S    WN LQ+AA+ ALD  E AL+  E+   LPKT DP  QI+GN+A
Sbjct: 62  PSPNPIIPEKDTS---RWNPLQRAASAALDFAETALLRRERSKPLPKTVDPRHQISGNYA 121

Query: 115 PVPEQSVRKVLPVTGKVPDCIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFTEGGRV 174
           PVPEQSV+  L V GK+PDCI GVY+RNGANPL EPVSGHHLFDGDGMVHAV+ T G   
Sbjct: 122 PVPEQSVKSSLSVDGKIPDCIDGVYLRNGANPLFEPVSGHHLFDGDGMVHAVKITNGD-A 181

Query: 175 SYACRFTETERLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHSHGIGVA 234
           SY+CRFTETERLVQE+  G P+FPKAIGELHGHSGIARLMLFYARGLFGL++H +G GVA
Sbjct: 182 SYSCRFTETERLVQEKQLGSPIFPKAIGELHGHSGIARLMLFYARGLFGLLNHKNGTGVA 241

Query: 235 NAGLVYFNGRLLAMSEDDLPYQIRVTPSGDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEM 294
           NAGLVYF+ RLLAMSEDDLPYQ+RVT +GDL+T+GRFDFDGQL S MIAHPK+DPV+ E+
Sbjct: 242 NAGLVYFHDRLLAMSEDDLPYQVRVTDNGDLETIGRFDFDGQLSSAMIAHPKIDPVTKEL 301

Query: 295 FALSYDVIQKPYLKYFKFSAEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVFKLP 354
           FALSYDV++KPYLKYFKFS EGEKSPDVEIPL  PTMMHDFAITE FVVIPDQQVVFKL 
Sbjct: 302 FALSYDVVKKPYLKYFKFSPEGEKSPDVEIPLASPTMMHDFAITENFVVIPDQQVVFKLS 361

Query: 355 EMIRGGSPVVYDKEKTSRFGILDKNATDANAIKWIEAPDCFCFHLWNAWEEPETNEIVVI 414
           +M  G SPV YD EK SRFGIL +NA DA+ + W+E+P+ FCFHLWNAWE PET+E+VVI
Sbjct: 362 DMFLGKSPVKYDGEKISRFGILPRNAKDASEMVWVESPETFCFHLWNAWESPETDEVVVI 421

Query: 415 GSCMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIIAETEQVNLEAGMVNRNQLGR 474
           GSCMTP DSIFNEC+E L SVLSEIRLNL TGKSTRR II  + Q+NLEAGMVNRN LGR
Sbjct: 422 GSCMTPADSIFNECDEQLNSVLSEIRLNLKTGKSTRRTIIPGSVQMNLEAGMVNRNLLGR 481

Query: 475 KTQFAYLALAEPWPKVSGFAKVDLSTGEIRKYLYGEDRYGGEPLFLPRE-GATAEDDGHI 534
           KT++AYLA+AEPWPKVSGFAKVDLSTGE++ + YG  +YGGEP FLPR   +  EDDG+I
Sbjct: 482 KTRYAYLAIAEPWPKVSGFAKVDLSTGEVKNHFYGGKKYGGEPFFLPRGLESDGEDDGYI 541

Query: 535 LAFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSKDLQKQ 585
           ++FVHDE+ W+SEL IVNA+TLELEATVKLPSRVPYGFHGTF++S D+  Q
Sbjct: 542 MSFVHDEESWESELHIVNAVTLELEATVKLPSRVPYGFHGTFVNSADMLNQ 588

BLAST of Cp4.1LG01g00800 vs. Swiss-Prot
Match: NCED_ONCHC (9-cis-epoxycarotenoid dioxygenase, chloroplastic OS=Oncidium hybrid cultivar GN=NCED PE=2 SV=1)

HSP 1 Score: 802.0 bits (2070), Expect = 4.4e-231
Identity = 394/569 (69.24%), Postives = 457/569 (80.32%), Query Frame = 1

Query: 22  VRNRVSCSLHTPSIIQIPKRSQTAFRSPIKIAVPK---PEPVAAPSVSSPAKEWNFLQQA 81
           +   + CS  TP+   +   S + F  P+    PK   PE  A PS  S    WN  Q+A
Sbjct: 48  IAGSIQCST-TPNSFGLDTTSPSPFY-PLPPTCPKEIHPEQSAKPSRPS----WNLFQRA 107

Query: 82  AAMALDAVEDALVS--MEKKHSLPKTADPSVQIAGNFAPVPEQSVRKVLPVTGKVPDCIR 141
           AA  L+AVED L+   +E  H LPKTADP+VQIAGNFAPV EQ     LPV G++P  I 
Sbjct: 108 AAAVLEAVEDNLIQNLLESGHPLPKTADPAVQIAGNFAPVGEQKPHHDLPVDGRIPPLIN 167

Query: 142 GVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFTEGGRVSYACRFTETERLVQERAYGRPV 201
           GVY+RNGANPL EPV+GHH FDGDGMVHAV     GR SYACRFTETERL QERA GR +
Sbjct: 168 GVYLRNGANPLFEPVAGHHFFDGDGMVHAVHL-RNGRASYACRFTETERLKQERAVGRAI 227

Query: 202 FPKAIGELHGHSGIARLMLFYARGLFGLVDHSHGIGVANAGLVYFNGRLLAMSEDDLPYQ 261
           FPKAIGELHGHSGIARL+LFYARGL GL+DHS G GVANAGL+YFN RLLAMSEDDLPY 
Sbjct: 228 FPKAIGELHGHSGIARLLLFYARGLLGLIDHSRGTGVANAGLIYFNNRLLAMSEDDLPYH 287

Query: 262 IRVTPSGDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEMFALSYDVIQKPYLKYFKFSAEG 321
           +R+ P+GDL+T GR+DFDGQL +TMIAHPKLDP + E FALSYDVI+KPYLKYF+FS  G
Sbjct: 288 VRIKPNGDLETAGRYDFDGQLTTTMIAHPKLDPETREFFALSYDVIKKPYLKYFRFSPCG 347

Query: 322 EKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVFKLPEMIRGGSPVVYDKEKTSRFGIL 381
           EKSPDVEIPLPQPTMMHDFAIT+ FV+IPDQQVVFKL EMI GGSPVVYDKEK +RFG+L
Sbjct: 348 EKSPDVEIPLPQPTMMHDFAITKNFVIIPDQQVVFKLQEMICGGSPVVYDKEKIARFGVL 407

Query: 382 DKNATDANAIKWIEAPDCFCFHLWNAWEEPETNEIVVIGSCMTPPDSIFNECEENLKSVL 441
            K A DA+ ++WI+ PDCFCFHLWN+WEEPET E+VVIGSCMTPPDSIFNE EENL+SVL
Sbjct: 408 PKYAIDASEMQWIDVPDCFCFHLWNSWEEPETEEVVVIGSCMTPPDSIFNESEENLQSVL 467

Query: 442 SEIRLNLSTGKSTRRPIIAE-TEQVNLEAGMVNRNQLGRKTQFAYLALAEPWPKVSGFAK 501
           +EIRLNL TGKSTRRPI+     Q+NLEAGMVNRN+LGR+T+FAYLA+AEPWPKVSGFAK
Sbjct: 468 TEIRLNLRTGKSTRRPILRPGNSQINLEAGMVNRNRLGRRTRFAYLAIAEPWPKVSGFAK 527

Query: 502 VDLSTGEIRKYLYGEDRYGGEPLFLPREGATAEDDGHILAFVHDEKEWKSELQIVNAMTL 561
           VDL++GEI+++ YG+  YGGEP F+PREG   ED G++LAFVHDE+E  SEL I+NA  +
Sbjct: 528 VDLASGEIQRFEYGDGGYGGEPYFVPREGCDREDGGYVLAFVHDEREGSSELLIMNAADM 587

Query: 562 ELEATVKLPSRVPYGFHGTFISSKDLQKQ 585
            LEA V+LPSRVPYGF+GTF+S+ +L  Q
Sbjct: 588 RLEAAVRLPSRVPYGFYGTFVSATELHSQ 609

BLAST of Cp4.1LG01g00800 vs. TrEMBL
Match: K4KBB8_MOMCH (9-cis-epoxycarotenoid dioxygenase OS=Momordica charantia GN=NCED PE=2 SV=1)

HSP 1 Score: 1041.2 bits (2691), Expect = 4.8e-301
Identity = 505/581 (86.92%), Postives = 543/581 (93.46%), Query Frame = 1

Query: 4   SSSSSSMPKILSVNRNYGVRNRVSCSLHTPSIIQIPKRSQTAFRSPIKIAVPKPEPVAAP 63
           S  S SMP++LSVN NY V+NRVSCSLHTPSI+QIPK SQT    P+K  V KP    +P
Sbjct: 12  SGFSPSMPELLSVNSNY-VKNRVSCSLHTPSIVQIPKHSQTFRPPPLKTPVEKP---VSP 71

Query: 64  SVSSPAKEWNFLQQAAAMALDAVEDALVSMEKKHSLPKTADPSVQIAGNFAPVPEQSVRK 123
           S S+P ++WNFLQ+AA+MA+DAVE+AL+S E+K  LPKTADP VQIAGNFAPVPEQ VRK
Sbjct: 72  STSAP-EDWNFLQRAASMAIDAVENALLSAERKRPLPKTADPEVQIAGNFAPVPEQPVRK 131

Query: 124 VLPVTGKVPDCIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFTEGGRVSYACRFTET 183
            LPVTG++P+CIRGVYVRNGANPLHEP +GHHLFDGDGMVHAVEF++GG VSYACRFTET
Sbjct: 132 ALPVTGRIPECIRGVYVRNGANPLHEPAAGHHLFDGDGMVHAVEFSDGGGVSYACRFTET 191

Query: 184 ERLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHSHGIGVANAGLVYFNG 243
           +RLVQERA+GRPVFPKAIGELHGHSGIARL+LFYARGLFGLVDHSHGIGVANAGLVYFNG
Sbjct: 192 QRLVQERAHGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHGIGVANAGLVYFNG 251

Query: 244 RLLAMSEDDLPYQIRVTPSGDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEMFALSYDVIQ 303
           RLLAMSEDDLPYQIRVTP+GDLKT+GRFDFDGQLKSTMIAHPKLDPVSGE+FA+SYDVIQ
Sbjct: 252 RLLAMSEDDLPYQIRVTPAGDLKTIGRFDFDGQLKSTMIAHPKLDPVSGELFAISYDVIQ 311

Query: 304 KPYLKYFKFSAEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVFKLPEMIRGGSPV 363
           KP+LKYFKFS EGEKSPDVE+PLPQPTMMHDFAITEKFVVIPDQQVVFKLPEMIRGGSPV
Sbjct: 312 KPHLKYFKFSPEGEKSPDVEVPLPQPTMMHDFAITEKFVVIPDQQVVFKLPEMIRGGSPV 371

Query: 364 VYDKEKTSRFGILDKNATDANAIKWIEAPDCFCFHLWNAWEEPETNEIVVIGSCMTPPDS 423
           +YDK K SRFGIL KNATDA  IKWIEAPDCFCFHLWNAWEEPETNE+VVIGSCMTPPDS
Sbjct: 372 IYDKNKISRFGILPKNATDAGDIKWIEAPDCFCFHLWNAWEEPETNEVVVIGSCMTPPDS 431

Query: 424 IFNECEENLKSVLSEIRLNLSTGKSTRRPIIAETEQVNLEAGMVNRNQLGRKTQFAYLAL 483
           IFNECEENLKSVLSEIRLNL+TGKSTRRPII ETEQVNLEAGMVNRN+LGRKTQFAYLAL
Sbjct: 432 IFNECEENLKSVLSEIRLNLTTGKSTRRPIIPETEQVNLEAGMVNRNRLGRKTQFAYLAL 491

Query: 484 AEPWPKVSGFAKVDLSTGEIRKYLYGEDRYGGEPLFLPREGATAEDDGHILAFVHDEKEW 543
           AEPWPKVSGFAKVDLSTGEIRKY+YGE RYGGEPLFLPREGA AEDDGHILAFVHDEKEW
Sbjct: 492 AEPWPKVSGFAKVDLSTGEIRKYIYGEQRYGGEPLFLPREGAEAEDDGHILAFVHDEKEW 551

Query: 544 KSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSKDLQKQ 585
           KSELQIVNA+TL+LEA+VKLPSRVPYGFHGTFI SK LQKQ
Sbjct: 552 KSELQIVNAITLKLEASVKLPSRVPYGFHGTFIGSKGLQKQ 587

BLAST of Cp4.1LG01g00800 vs. TrEMBL
Match: F6HR90_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_19s0093g00550 PE=4 SV=1)

HSP 1 Score: 932.2 bits (2408), Expect = 3.2e-268
Identity = 452/586 (77.13%), Postives = 517/586 (88.23%), Query Frame = 1

Query: 2   ASSSSSSSMPKILSVN-RNYGVRNRVSCSLHTPSIIQIPKRSQTAFR-SPIKIAVPKPEP 61
           +SSSSSSS   + S +     +R  ++CSLH+PS++  PK + ++   + +K  +PK + 
Sbjct: 27  SSSSSSSSARDLGSFSFHRPTIRPNINCSLHSPSVLHFPKHTSSSTGVATVKTTLPKEKD 86

Query: 62  VAAPSVSSPAKEWNFLQQAAAMALDAVEDALVSMEKKHSLPKTADPSVQIAGNFAPVPEQ 121
             + SVS    +WNFLQ AAAMALDAVE ALV+ E +H LPKTADP VQIAGNFAPVPEQ
Sbjct: 87  GFSSSVSP---QWNFLQSAAAMALDAVETALVARETQHPLPKTADPRVQIAGNFAPVPEQ 146

Query: 122 SVRKVLPVTGKVPDCIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFTEGGRVSYACR 181
            V   LPV G++PDCI+GVY RNGANPLH+PV+GHHLFDGDGMVHAV+F +G   SYACR
Sbjct: 147 PVHHSLPVDGRIPDCIQGVYTRNGANPLHKPVAGHHLFDGDGMVHAVQFKDGD-ASYACR 206

Query: 182 FTETERLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHSHGIGVANAGLV 241
           FTET+RL+QER +GRPVFPKAIGELHGHSGIARL+LFYARGLFGLVDHSHG GVANAGLV
Sbjct: 207 FTETQRLIQERDFGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHGTGVANAGLV 266

Query: 242 YFNGRLLAMSEDDLPYQIRVTPSGDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEMFALSY 301
           YFN RLLAMSEDDLPYQIRVTPSGDL+TVGR+DF+GQL+STMIAHPK+DPVSGEMFALSY
Sbjct: 267 YFNRRLLAMSEDDLPYQIRVTPSGDLETVGRYDFEGQLRSTMIAHPKVDPVSGEMFALSY 326

Query: 302 DVIQKPYLKYFKFSAEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVFKLPEMIRG 361
           DV+QKPYLKYF+FS +G+KSPDVEIPL +PTMMHDFAITE+FVV+PDQQVVFKL EMI G
Sbjct: 327 DVVQKPYLKYFRFSPKGDKSPDVEIPLAEPTMMHDFAITERFVVVPDQQVVFKLQEMISG 386

Query: 362 GSPVVYDKEKTSRFGILDKNATDANAIKWIEAPDCFCFHLWNAWEEPETNEIVVIGSCMT 421
           GSPVVY+K K SRFG+LDKNATDA+ ++WIEAPDCFCFHLWNAWEEPET+E+VVIGSCMT
Sbjct: 387 GSPVVYEKNKMSRFGVLDKNATDASGMRWIEAPDCFCFHLWNAWEEPETDEVVVIGSCMT 446

Query: 422 PPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIIAETEQVNLEAGMVNRNQLGRKTQFA 481
           PPDSIFNEC+E LKSVLSEIRLNL TGKSTRRPI+ E+EQVNLEAGMVNRN+LGRKTQFA
Sbjct: 447 PPDSIFNECDEGLKSVLSEIRLNLKTGKSTRRPILPESEQVNLEAGMVNRNRLGRKTQFA 506

Query: 482 YLALAEPWPKVSGFAKVDLSTGEIRKYLYGEDRYGGEPLFLPRE-GATAEDDGHILAFVH 541
           YLA+AEPWPKVSGFAKVDLSTG++ KY+YGE RYGGEPLFLPR+  +  EDDG+ILAFVH
Sbjct: 507 YLAVAEPWPKVSGFAKVDLSTGQVSKYIYGEQRYGGEPLFLPRDPNSGREDDGYILAFVH 566

Query: 542 DEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSKDLQKQ 585
           DEK WKSELQIVNA  L+LEA+VKLPSRVPYGFHGTFISSK+L+KQ
Sbjct: 567 DEKTWKSELQIVNATNLQLEASVKLPSRVPYGFHGTFISSKELEKQ 608

BLAST of Cp4.1LG01g00800 vs. TrEMBL
Match: A5BR72_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_018449 PE=4 SV=1)

HSP 1 Score: 932.2 bits (2408), Expect = 3.2e-268
Identity = 452/589 (76.74%), Postives = 517/589 (87.78%), Query Frame = 1

Query: 2   ASSSSSSSMPKILSVN----RNYGVRNRVSCSLHTPSIIQIPKRSQTAFR-SPIKIAVPK 61
           +SSSSSSS    L +         +R  ++CSLH+PS++  PK + ++   + +K  +PK
Sbjct: 25  SSSSSSSSSSSALDLGSFSFHRPTIRPNINCSLHSPSVLHFPKHTSSSTGVATVKTTLPK 84

Query: 62  PEPVAAPSVSSPAKEWNFLQQAAAMALDAVEDALVSMEKKHSLPKTADPSVQIAGNFAPV 121
            +   + SVS   ++WNFLQ AAAMALDAVE ALV+ E +H LPKTADP VQIAGNFAPV
Sbjct: 85  EKDDFSSSVS---QQWNFLQSAAAMALDAVETALVARETQHPLPKTADPRVQIAGNFAPV 144

Query: 122 PEQSVRKVLPVTGKVPDCIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFTEGGRVSY 181
           PEQ V+  LPV G++PDCI+GVY RNGANPLH+PV+GHHLFDGDGMVHAV+F +G   SY
Sbjct: 145 PEQPVQHSLPVDGRIPDCIQGVYTRNGANPLHKPVAGHHLFDGDGMVHAVQFKDGD-ASY 204

Query: 182 ACRFTETERLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHSHGIGVANA 241
           ACRFTET+RL+QER +GRPVFPKAIGELHGHSGIARL+LFYARGLFGLVDHSHG GVANA
Sbjct: 205 ACRFTETQRLIQERDFGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHGTGVANA 264

Query: 242 GLVYFNGRLLAMSEDDLPYQIRVTPSGDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEMFA 301
           GLVYFN RLLAMSEDDLPYQIRVTPSGDL+TVGR+DF+GQL+STMIAHPK+DPVSGEMFA
Sbjct: 265 GLVYFNRRLLAMSEDDLPYQIRVTPSGDLETVGRYDFEGQLRSTMIAHPKVDPVSGEMFA 324

Query: 302 LSYDVIQKPYLKYFKFSAEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVFKLPEM 361
           LSYDV+QKPYLKYF+FS +G+KSPDVEIPL +PTMMHDFAITE+FVV+P+QQVVFKL EM
Sbjct: 325 LSYDVVQKPYLKYFRFSPKGDKSPDVEIPLAEPTMMHDFAITERFVVVPNQQVVFKLQEM 384

Query: 362 IRGGSPVVYDKEKTSRFGILDKNATDANAIKWIEAPDCFCFHLWNAWEEPETNEIVVIGS 421
           I GGSPVVYDK K SRFG+LDKNATDA+ + WIEAPDCFCFHLWNAWEEPET+E+VVIGS
Sbjct: 385 ISGGSPVVYDKNKMSRFGVLDKNATDASGMXWIEAPDCFCFHLWNAWEEPETDEVVVIGS 444

Query: 422 CMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIIAETEQVNLEAGMVNRNQLGRKT 481
           CMTPPDSIFNEC+E LKSVLSEIRLNL TGKSTRRPI+ E+EQVNLEAGMVNRN+LGRKT
Sbjct: 445 CMTPPDSIFNECDEGLKSVLSEIRLNLKTGKSTRRPILPESEQVNLEAGMVNRNRLGRKT 504

Query: 482 QFAYLALAEPWPKVSGFAKVDLSTGEIRKYLYGEDRYGGEPLFLPRE-GATAEDDGHILA 541
           QFAYLA+AEPWPKVSGFAKVDLSTG++ KY+YGE RYGGEPLFLPR+  +  EDDG+ILA
Sbjct: 505 QFAYLAVAEPWPKVSGFAKVDLSTGQVSKYIYGEQRYGGEPLFLPRDPNSGREDDGYILA 564

Query: 542 FVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSKDLQKQ 585
           FVHDEK WKSELQIVNA  L+LEA+VKLPSRVPYGFHGTFISSK+L+KQ
Sbjct: 565 FVHDEKTWKSELQIVNATNLQLEASVKLPSRVPYGFHGTFISSKELEKQ 609

BLAST of Cp4.1LG01g00800 vs. TrEMBL
Match: I7FMA3_VITVI (9-cis epoxy carotenoid dioxygenase 3 OS=Vitis vinifera GN=NCED3 PE=2 SV=1)

HSP 1 Score: 932.2 bits (2408), Expect = 3.2e-268
Identity = 452/589 (76.74%), Postives = 517/589 (87.78%), Query Frame = 1

Query: 2   ASSSSSSSMPKILSVN----RNYGVRNRVSCSLHTPSIIQIPKRSQTAFR-SPIKIAVPK 61
           +SSSSSSS    L +         +R  ++CSLH+PS++  PK + ++   + +K  +PK
Sbjct: 25  SSSSSSSSSSSALDLGSFSFHRPTIRPNINCSLHSPSVLHFPKHTSSSTGVATVKTTLPK 84

Query: 62  PEPVAAPSVSSPAKEWNFLQQAAAMALDAVEDALVSMEKKHSLPKTADPSVQIAGNFAPV 121
            +   + SVS   ++WNFLQ AAAMALDAVE ALV+ E +H LPKTADP VQIAGNFAPV
Sbjct: 85  EKDDFSSSVS---QQWNFLQSAAAMALDAVETALVARETQHPLPKTADPRVQIAGNFAPV 144

Query: 122 PEQSVRKVLPVTGKVPDCIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFTEGGRVSY 181
           PEQ V+  LPV G++PDCI+GVY RNGANPLH+PV+GHHLFDGDGMVHAV+F +G   SY
Sbjct: 145 PEQPVQHSLPVDGRIPDCIQGVYTRNGANPLHKPVAGHHLFDGDGMVHAVQFKDGD-ASY 204

Query: 182 ACRFTETERLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHSHGIGVANA 241
           ACRFTET+RL+QER +GRPVFPKAIGELHGHSGIARL+LFYARGLFGLVDHSHG GVANA
Sbjct: 205 ACRFTETQRLIQERDFGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHGTGVANA 264

Query: 242 GLVYFNGRLLAMSEDDLPYQIRVTPSGDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEMFA 301
           GLVYFN RLLAMSEDDLPYQIRVTPSGDL+TVGR+DF+GQL+STMIAHPK+DPVSGEMFA
Sbjct: 265 GLVYFNRRLLAMSEDDLPYQIRVTPSGDLETVGRYDFEGQLRSTMIAHPKVDPVSGEMFA 324

Query: 302 LSYDVIQKPYLKYFKFSAEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVFKLPEM 361
           LSYDV+QKPYLKYF+FS +G+KSPDVEIPL +PTMMHDFAITE+FVV+PDQQVVFKL EM
Sbjct: 325 LSYDVVQKPYLKYFRFSPKGDKSPDVEIPLAEPTMMHDFAITERFVVVPDQQVVFKLQEM 384

Query: 362 IRGGSPVVYDKEKTSRFGILDKNATDANAIKWIEAPDCFCFHLWNAWEEPETNEIVVIGS 421
           I  GSPVVYDK K SRFG+LDKNATDA+ ++WIEAPDCFCFHLWNAWEEPET+E+VVIGS
Sbjct: 385 ISRGSPVVYDKNKMSRFGVLDKNATDASGMRWIEAPDCFCFHLWNAWEEPETDEVVVIGS 444

Query: 422 CMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIIAETEQVNLEAGMVNRNQLGRKT 481
           CMTPPDSIFNEC+E LKSVLSEIRLNL TGKSTRRPI+ E+EQVNLEAGMVNRN+LGRKT
Sbjct: 445 CMTPPDSIFNECDEGLKSVLSEIRLNLKTGKSTRRPILPESEQVNLEAGMVNRNRLGRKT 504

Query: 482 QFAYLALAEPWPKVSGFAKVDLSTGEIRKYLYGEDRYGGEPLFLPRE-GATAEDDGHILA 541
           QFAYLA+AEPWPKVSGFAKVDLSTG++ KY+YGE RYGGEPLFLPR+  +  EDDG+ILA
Sbjct: 505 QFAYLAVAEPWPKVSGFAKVDLSTGQVSKYIYGEQRYGGEPLFLPRDPNSGREDDGYILA 564

Query: 542 FVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSKDLQKQ 585
           FVHDEK WKSELQIVNA  L+LEA+VKLPSRVPYGFHGTFISSK+L+KQ
Sbjct: 565 FVHDEKTWKSELQIVNATNLQLEASVKLPSRVPYGFHGTFISSKELEKQ 609

BLAST of Cp4.1LG01g00800 vs. TrEMBL
Match: Q5SGD1_VITVI (9-cis-epoxycarotenoid dioxygenase 1 OS=Vitis vinifera GN=NCED1 PE=2 SV=1)

HSP 1 Score: 931.8 bits (2407), Expect = 4.1e-268
Identity = 452/589 (76.74%), Postives = 517/589 (87.78%), Query Frame = 1

Query: 2   ASSSSSSSMPKILSVN----RNYGVRNRVSCSLHTPSIIQIPKRSQTAFR-SPIKIAVPK 61
           +SSSSSSS    L +         +R  ++CSLH+PS++  PK + ++   + +K  +PK
Sbjct: 25  SSSSSSSSSSSALDLGSFSFHRPTIRPNINCSLHSPSVLHFPKHTSSSTGVATVKTTLPK 84

Query: 62  PEPVAAPSVSSPAKEWNFLQQAAAMALDAVEDALVSMEKKHSLPKTADPSVQIAGNFAPV 121
            +   + SVS   ++WNFLQ AAAMALDAVE ALV+ E +H LPKTADP VQIAGNFAPV
Sbjct: 85  EKDDFSSSVS---QQWNFLQSAAAMALDAVETALVARETQHPLPKTADPRVQIAGNFAPV 144

Query: 122 PEQSVRKVLPVTGKVPDCIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFTEGGRVSY 181
           PEQ V+  LPV G++PDCI+GVY RNGANPLH+PV+GHHLFDGDGMVHAV+F +G   SY
Sbjct: 145 PEQPVQHSLPVDGRIPDCIQGVYTRNGANPLHKPVAGHHLFDGDGMVHAVQFKDGD-ASY 204

Query: 182 ACRFTETERLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHSHGIGVANA 241
           ACRFTET+RL+QER +GRPVFPKAIGELHGHSGIARL+LFYARGLFGLVDHSHG GVANA
Sbjct: 205 ACRFTETQRLIQERDFGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHGTGVANA 264

Query: 242 GLVYFNGRLLAMSEDDLPYQIRVTPSGDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEMFA 301
           GLVYFN RLLAMSEDDLPYQIRVTPSGDL+TVGR+DF+GQL+STMIAHPK+DPVSGEMFA
Sbjct: 265 GLVYFNRRLLAMSEDDLPYQIRVTPSGDLETVGRYDFEGQLRSTMIAHPKVDPVSGEMFA 324

Query: 302 LSYDVIQKPYLKYFKFSAEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVFKLPEM 361
           LSYDV+QKPYLKYF+FS +G+KSPDVEIPL +PTMMHDFAITE+FVV+P+QQVVFKL EM
Sbjct: 325 LSYDVVQKPYLKYFRFSPKGDKSPDVEIPLAEPTMMHDFAITERFVVVPNQQVVFKLQEM 384

Query: 362 IRGGSPVVYDKEKTSRFGILDKNATDANAIKWIEAPDCFCFHLWNAWEEPETNEIVVIGS 421
           I GGSPVVYDK K SRFG+LDKNATDA+ + WIEAPDCFCFHLWNAWEEPET+E+VVIGS
Sbjct: 385 ISGGSPVVYDKNKMSRFGVLDKNATDASGMLWIEAPDCFCFHLWNAWEEPETDEVVVIGS 444

Query: 422 CMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIIAETEQVNLEAGMVNRNQLGRKT 481
           CMTPPDSIFNEC+E LKSVLSEIRLNL TGKSTRRPI+ E+EQVNLEAGMVNRN+LGRKT
Sbjct: 445 CMTPPDSIFNECDEGLKSVLSEIRLNLKTGKSTRRPILPESEQVNLEAGMVNRNRLGRKT 504

Query: 482 QFAYLALAEPWPKVSGFAKVDLSTGEIRKYLYGEDRYGGEPLFLPRE-GATAEDDGHILA 541
           QFAYLA+AEPWPKVSGFAKVDLSTG++ KY+YGE RYGGEPLFLPR+  +  EDDG+ILA
Sbjct: 505 QFAYLAVAEPWPKVSGFAKVDLSTGQVSKYIYGEQRYGGEPLFLPRDPNSGREDDGYILA 564

Query: 542 FVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSKDLQKQ 585
           FVHDEK WKSELQIVNA  L+LEA+VKLPSRVPYGFHGTFISSK+L+KQ
Sbjct: 565 FVHDEKTWKSELQIVNATNLQLEASVKLPSRVPYGFHGTFISSKELEKQ 609

BLAST of Cp4.1LG01g00800 vs. TAIR10
Match: AT3G14440.1 (AT3G14440.1 nine-cis-epoxycarotenoid dioxygenase 3)

HSP 1 Score: 877.1 bits (2265), Expect = 6.1e-255
Identity = 439/590 (74.41%), Postives = 492/590 (83.39%), Query Frame = 1

Query: 1   MASSSSS-----SSMPKILSVNRNYGVRNRVSCSLHTPSIIQIPKRSQTAFRSPIKIAVP 60
           ++SS SS     SS+P    V R   V    S +LHTP  +  PK+S     SP  +  P
Sbjct: 24  LSSSQSSDLSYCSSLPMASRVTRKLNV----SSALHTPPALHFPKQSSN---SPAIVVKP 83

Query: 61  KPEPVAAPSVSSPAKEWNFLQQAAAMALDAVEDALVSMEKKHSLPKTADPSVQIAGNFAP 120
           K +        S  K+ N  Q+AAA ALDA E  LVS EK H LPKTADPSVQIAGNFAP
Sbjct: 84  KAK-------ESNTKQMNLFQRAAAAALDAAEGFLVSHEKLHPLPKTADPSVQIAGNFAP 143

Query: 121 VPEQSVRKVLPVTGKVPDCIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFTEGGRVS 180
           V EQ VR+ LPV GK+PD I+GVYVRNGANPLHEPV+GHH FDGDGMVHAV+F E G  S
Sbjct: 144 VNEQPVRRNLPVVGKLPDSIKGVYVRNGANPLHEPVTGHHFFDGDGMVHAVKF-EHGSAS 203

Query: 181 YACRFTETERLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHSHGIGVAN 240
           YACRFT+T R VQER  GRPVFPKAIGELHGH+GIARLMLFYAR   G+VD +HG GVAN
Sbjct: 204 YACRFTQTNRFVQERQLGRPVFPKAIGELHGHTGIARLMLFYARAAAGIVDPAHGTGVAN 263

Query: 241 AGLVYFNGRLLAMSEDDLPYQIRVTPSGDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEMF 300
           AGLVYFNGRLLAMSEDDLPYQ+++TP+GDLKTVGRFDFDGQL+STMIAHPK+DP SGE+F
Sbjct: 264 AGLVYFNGRLLAMSEDDLPYQVQITPNGDLKTVGRFDFDGQLESTMIAHPKVDPESGELF 323

Query: 301 ALSYDVIQKPYLKYFKFSAEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVFKLPE 360
           ALSYDV+ KPYLKYF+FS +G KSPDVEI L QPTMMHDFAITE FVV+PDQQVVFKLPE
Sbjct: 324 ALSYDVVSKPYLKYFRFSPDGTKSPDVEIQLDQPTMMHDFAITENFVVVPDQQVVFKLPE 383

Query: 361 MIRGGSPVVYDKEKTSRFGILDKNATDANAIKWIEAPDCFCFHLWNAWEEPETNEIVVIG 420
           MIRGGSPVVYDK K +RFGILDK A D++ IKWI+APDCFCFHLWNAWEEPET+E+VVIG
Sbjct: 384 MIRGGSPVVYDKNKVARFGILDKYAEDSSNIKWIDAPDCFCFHLWNAWEEPETDEVVVIG 443

Query: 421 SCMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIIA-ETEQVNLEAGMVNRNQLGR 480
           SCMTPPDSIFNE +ENLKSVLSEIRLNL TG+STRRPII+ E +QVNLEAGMVNRN LGR
Sbjct: 444 SCMTPPDSIFNESDENLKSVLSEIRLNLKTGESTRRPIISNEDQQVNLEAGMVNRNMLGR 503

Query: 481 KTQFAYLALAEPWPKVSGFAKVDLSTGEIRKYLYGEDRYGGEPLFLPREGATAEDDGHIL 540
           KT+FAYLALAEPWPKVSGFAKVDL+TGE++K+LYG++RYGGEPLFLP EG   ED+G+IL
Sbjct: 504 KTKFAYLALAEPWPKVSGFAKVDLTTGEVKKHLYGDNRYGGEPLFLPGEGG-EEDEGYIL 563

Query: 541 AFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSKDLQKQ 585
            FVHDEK WKSELQIVNA++LE+EATVKLPSRVPYGFHGTFI + DL KQ
Sbjct: 564 CFVHDEKTWKSELQIVNAVSLEVEATVKLPSRVPYGFHGTFIGADDLAKQ 597

BLAST of Cp4.1LG01g00800 vs. TAIR10
Match: AT1G78390.1 (AT1G78390.1 nine-cis-epoxycarotenoid dioxygenase 9)

HSP 1 Score: 833.9 bits (2153), Expect = 5.9e-242
Identity = 417/590 (70.68%), Postives = 492/590 (83.39%), Query Frame = 1

Query: 3   SSSSSSSMPKILSVNRNYGVRNR-------VSCSLHTPSIIQIPKRSQTAFRSPIKIAVP 62
           S+SSSSS PK+ S++ +  +RN+       VS S++  S +    +S T F+ P    + 
Sbjct: 70  STSSSSSRPKLQSLSFSSTLRNKKLVVPCYVSSSVNKKSSVSSSLQSPT-FKPPSWKKLC 129

Query: 63  KPEPVAAPSVSSPAKEWNFLQQAAAMALDAVEDALVSMEKK-HSLPKTADPSVQIAGNFA 122
                  P  ++   + N +Q+ AAM LDAVE+A++S E++ H  PKTADP+VQIAGNF 
Sbjct: 130 NDVTNLIPKTTNQNPKLNPVQRTAAMVLDAVENAMISHERRRHPHPKTADPAVQIAGNFF 189

Query: 123 PVPEQSVRKVLPVTGKVPDCIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFTEGGRV 182
           PVPE+ V   LPVTG VP+CI+GVYVRNGANPLH+PVSGHHLFDGDGMVHAV F + G V
Sbjct: 190 PVPEKPVVHNLPVTGTVPECIQGVYVRNGANPLHKPVSGHHLFDGDGMVHAVRF-DNGSV 249

Query: 183 SYACRFTETERLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHSHGIGVA 242
           SYACRFTET RLVQER  GRPVFPKAIGELHGH GIA+LMLF  RGLFGLVD + G+GVA
Sbjct: 250 SYACRFTETNRLVQERECGRPVFPKAIGELHGHLGIAKLMLFNTRGLFGLVDPTGGLGVA 309

Query: 243 NAGLVYFNGRLLAMSEDDLPYQIRVTPSGDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEM 302
           NAGLVYFNG LLAMSEDDLPY ++VT +GDL+T GR+DFDGQLKSTMIAHPK+DP + E+
Sbjct: 310 NAGLVYFNGHLLAMSEDDLPYHVKVTQTGDLETSGRYDFDGQLKSTMIAHPKIDPETREL 369

Query: 303 FALSYDVIQKPYLKYFKFSAEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVFKLP 362
           FALSYDV+ KPYLKYF+F+++GEKSPDVEIPL QPTM+HDFAITE FVVIPDQQVVF+LP
Sbjct: 370 FALSYDVVSKPYLKYFRFTSDGEKSPDVEIPLDQPTMIHDFAITENFVVIPDQQVVFRLP 429

Query: 363 EMIRGGSPVVYDKEKTSRFGILDKNATDANAIKWIEAPDCFCFHLWNAWEEPETNEIVVI 422
           EMIRGGSPVVYD++K SRFGIL+KNA DA++I+WIE PDCFCFHLWN+WEEPET+E+VVI
Sbjct: 430 EMIRGGSPVVYDEKKKSRFGILNKNAKDASSIQWIEVPDCFCFHLWNSWEEPETDEVVVI 489

Query: 423 GSCMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIIAETEQVNLEAGMVNRNQLGR 482
           GSCMTPPDSIFNE +E L+SVLSEIRLNL TG+STRRP+I  +EQVNLEAGMVNRN LGR
Sbjct: 490 GSCMTPPDSIFNEHDETLQSVLSEIRLNLKTGESTRRPVI--SEQVNLEAGMVNRNLLGR 549

Query: 483 KTQFAYLALAEPWPKVSGFAKVDLSTGEIRKYLYGEDRYGGEPLFLPREGATAEDDGHIL 542
           KT++AYLAL EPWPKVSGFAKVDLSTGEIRKY+YGE +YGGEPLFLP  G   ED G+I+
Sbjct: 550 KTRYAYLALTEPWPKVSGFAKVDLSTGEIRKYIYGEGKYGGEPLFLP-SGDGEEDGGYIM 609

Query: 543 AFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSKDLQKQ 585
            FVHDE++ KSELQ++NA+ ++LEATV LPSRVPYGFHGTFIS +DL KQ
Sbjct: 610 VFVHDEEKVKSELQLINAVNMKLEATVTLPSRVPYGFHGTFISKEDLSKQ 654

BLAST of Cp4.1LG01g00800 vs. TAIR10
Match: AT1G30100.1 (AT1G30100.1 nine-cis-epoxycarotenoid dioxygenase 5)

HSP 1 Score: 807.4 bits (2084), Expect = 5.9e-234
Identity = 388/531 (73.07%), Postives = 445/531 (83.80%), Query Frame = 1

Query: 55  PKPEPVAAPSVSSPAKEWNFLQQAAAMALDAVEDALVSMEKKHSLPKTADPSVQIAGNFA 114
           P P P+     +S    WN LQ+AA+ ALD  E AL+  E+   LPKT DP  QI+GN+A
Sbjct: 62  PSPNPIIPEKDTS---RWNPLQRAASAALDFAETALLRRERSKPLPKTVDPRHQISGNYA 121

Query: 115 PVPEQSVRKVLPVTGKVPDCIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFTEGGRV 174
           PVPEQSV+  L V GK+PDCI GVY+RNGANPL EPVSGHHLFDGDGMVHAV+ T G   
Sbjct: 122 PVPEQSVKSSLSVDGKIPDCIDGVYLRNGANPLFEPVSGHHLFDGDGMVHAVKITNGD-A 181

Query: 175 SYACRFTETERLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHSHGIGVA 234
           SY+CRFTETERLVQE+  G P+FPKAIGELHGHSGIARLMLFYARGLFGL++H +G GVA
Sbjct: 182 SYSCRFTETERLVQEKQLGSPIFPKAIGELHGHSGIARLMLFYARGLFGLLNHKNGTGVA 241

Query: 235 NAGLVYFNGRLLAMSEDDLPYQIRVTPSGDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEM 294
           NAGLVYF+ RLLAMSEDDLPYQ+RVT +GDL+T+GRFDFDGQL S MIAHPK+DPV+ E+
Sbjct: 242 NAGLVYFHDRLLAMSEDDLPYQVRVTDNGDLETIGRFDFDGQLSSAMIAHPKIDPVTKEL 301

Query: 295 FALSYDVIQKPYLKYFKFSAEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVFKLP 354
           FALSYDV++KPYLKYFKFS EGEKSPDVEIPL  PTMMHDFAITE FVVIPDQQVVFKL 
Sbjct: 302 FALSYDVVKKPYLKYFKFSPEGEKSPDVEIPLASPTMMHDFAITENFVVIPDQQVVFKLS 361

Query: 355 EMIRGGSPVVYDKEKTSRFGILDKNATDANAIKWIEAPDCFCFHLWNAWEEPETNEIVVI 414
           +M  G SPV YD EK SRFGIL +NA DA+ + W+E+P+ FCFHLWNAWE PET+E+VVI
Sbjct: 362 DMFLGKSPVKYDGEKISRFGILPRNAKDASEMVWVESPETFCFHLWNAWESPETDEVVVI 421

Query: 415 GSCMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIIAETEQVNLEAGMVNRNQLGR 474
           GSCMTP DSIFNEC+E L SVLSEIRLNL TGKSTRR II  + Q+NLEAGMVNRN LGR
Sbjct: 422 GSCMTPADSIFNECDEQLNSVLSEIRLNLKTGKSTRRTIIPGSVQMNLEAGMVNRNLLGR 481

Query: 475 KTQFAYLALAEPWPKVSGFAKVDLSTGEIRKYLYGEDRYGGEPLFLPRE-GATAEDDGHI 534
           KT++AYLA+AEPWPKVSGFAKVDLSTGE++ + YG  +YGGEP FLPR   +  EDDG+I
Sbjct: 482 KTRYAYLAIAEPWPKVSGFAKVDLSTGEVKNHFYGGKKYGGEPFFLPRGLESDGEDDGYI 541

Query: 535 LAFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSKDLQKQ 585
           ++FVHDE+ W+SEL IVNA+TLELEATVKLPSRVPYGFHGTF++S D+  Q
Sbjct: 542 MSFVHDEESWESELHIVNAVTLELEATVKLPSRVPYGFHGTFVNSADMLNQ 588

BLAST of Cp4.1LG01g00800 vs. TAIR10
Match: AT4G18350.1 (AT4G18350.1 nine-cis-epoxycarotenoid dioxygenase 2)

HSP 1 Score: 765.4 bits (1975), Expect = 2.6e-221
Identity = 369/531 (69.49%), Postives = 439/531 (82.67%), Query Frame = 1

Query: 57  PEPVAAPSVSSPAKEWNFLQQAAAMALDAVEDALVSMEKKHSLPKTADPSVQIAGNFAPV 116
           P   A P   +P +  N  Q+AAA+A+DA E AL+S E+   LPKTADP VQIAGN++PV
Sbjct: 55  PRTTATPPQHNPLR-LNIFQKAAAIAIDAAERALISHEQDSPLPKTADPRVQIAGNYSPV 114

Query: 117 PEQSVRKVLPVTGKVPDCIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFTEGGRVSY 176
           PE SVR+ L V G +PDCI GVY+RNGANP+ EP +GHHLFDGDGMVHAV+ T G   SY
Sbjct: 115 PESSVRRNLTVEGTIPDCIDGVYIRNGANPMFEPTAGHHLFDGDGMVHAVKITNGS-ASY 174

Query: 177 ACRFTETERLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHSHGIGVANA 236
           ACRFT+TERLVQE+  GRPVFPKAIGELHGHSGIARLMLFYARGL GL+++ +G+GVANA
Sbjct: 175 ACRFTKTERLVQEKRLGRPVFPKAIGELHGHSGIARLMLFYARGLCGLINNQNGVGVANA 234

Query: 237 GLVYFNGRLLAMSEDDLPYQIRVTPSGDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEMFA 296
           GLVYFN RLLAMSEDDLPYQ+++T +GDL+TVGR+DFDGQLKS MIAHPKLDPV+ E+ A
Sbjct: 235 GLVYFNNRLLAMSEDDLPYQLKITQTGDLQTVGRYDFDGQLKSAMIAHPKLDPVTKELHA 294

Query: 297 LSYDVIQKPYLKYFKFSAEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVFKLPEM 356
           LSYDV++KPYLKYF+FS +G KSP++EIPL  PTM+HDFAITE FVVIPDQQVVFKL EM
Sbjct: 295 LSYDVVKKPYLKYFRFSPDGVKSPELEIPLETPTMIHDFAITENFVVIPDQQVVFKLGEM 354

Query: 357 IRGGSPVVYDKEKTSRFGILDKNATDANAIKWIEAPDCFCFHLWNAWEEPETNEIVVIGS 416
           I G SPVV+D EK SR GI+ K+AT+A+ I W+ +P+ FCFHLWNAWE PET EIVVIGS
Sbjct: 355 ISGKSPVVFDGEKVSRLGIMPKDATEASQIIWVNSPETFCFHLWNAWESPETEEIVVIGS 414

Query: 417 CMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIIAETEQVNLEAGMVNRNQLGRKT 476
           CM+P DSIFNE +E+L+SVLSEIR+NL T K+TRR ++   E VNLE GMVNRN+LGRKT
Sbjct: 415 CMSPADSIFNERDESLRSVLSEIRINLRTRKTTRRSLLV-NEDVNLEIGMVNRNRLGRKT 474

Query: 477 QFAYLALAEPWPKVSGFAKVDLSTGEIRKYLYGEDRYGGEPLFLP---REGATAEDDGHI 536
           +FA+LA+A PWPKVSGFAKVDL TGE++KY+YG ++YGGEP FLP     G   EDDG+I
Sbjct: 475 RFAFLAIAYPWPKVSGFAKVDLCTGEMKKYIYGGEKYGGEPFFLPGNSGNGEENEDDGYI 534

Query: 537 LAFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSKDLQKQ 585
              VHDE+   SELQI+NA+ L+LEAT+KLPSRVPYGFHGTF+ S +L  Q
Sbjct: 535 FCHVHDEETKTSELQIINAVNLKLEATIKLPSRVPYGFHGTFVDSNELVDQ 582

BLAST of Cp4.1LG01g00800 vs. TAIR10
Match: AT3G24220.1 (AT3G24220.1 nine-cis-epoxycarotenoid dioxygenase 6)

HSP 1 Score: 639.8 bits (1649), Expect = 1.6e-183
Identity = 307/532 (57.71%), Postives = 399/532 (75.00%), Query Frame = 1

Query: 59  PVAAPSVSSPA-KEWNFLQQAAAMALDAVEDALV-SMEKKHSLPKTADPSVQIAGNFAPV 118
           PV +P    P     N LQ+ AA  LD +E ++V  ME+   LPK  DP+VQ++GNFAPV
Sbjct: 49  PVPSPVKLKPTYPNLNLLQKLAATMLDKIESSIVIPMEQNRPLPKPTDPAVQLSGNFAPV 108

Query: 119 PEQSVRKVLPVTGKVPDCIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFTEGGRVSY 178
            E  V+  L V G++P C++GVY+RNGANP+  P++GHHLFDGDGM+HAV      +VSY
Sbjct: 109 NECPVQNGLEVVGQIPSCLKGVYIRNGANPMFPPLAGHHLFDGDGMIHAVSIGFDNQVSY 168

Query: 179 ACRFTETERLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHSHGIGVANA 238
           +CR+T+T RLVQE A GR VFPK IGELHGHSG+ARL LF AR   GLVD + G+GVANA
Sbjct: 169 SCRYTKTNRLVQETALGRSVFPKPIGELHGHSGLARLALFTARAGIGLVDGTRGMGVANA 228

Query: 239 GLVYFNGRLLAMSEDDLPYQIRVTPSGDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEMFA 298
           G+V+FNGRLLAMSEDDLPYQ+++   GDL+T+GRF FD Q+ S++IAHPK+D  +G++  
Sbjct: 229 GVVFFNGRLLAMSEDDLPYQVKIDGQGDLETIGRFGFDDQIDSSVIAHPKVDATTGDLHT 288

Query: 299 LSYDVIQKPYLKYFKFSAEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVFKLPEM 358
           LSY+V++KP+L+Y KF+  G+K+ DVEI LP+PTM+HDFAITE FVVIPDQQ+VFKL EM
Sbjct: 289 LSYNVLKKPHLRYLKFNTCGKKTRDVEITLPEPTMIHDFAITENFVVIPDQQMVFKLSEM 348

Query: 359 IRGGSPVVYDKEKTSRFGILDKNATDANAIKWIEAPDCFCFHLWNAWEEPETNE----IV 418
           IRGGSPV+Y KEK +RFG+L K     + I W++ PDCFCFHLWNAWEE  T E    IV
Sbjct: 349 IRGGSPVIYVKEKMARFGVLSKQDLTGSDINWVDVPDCFCFHLWNAWEE-RTEEGDPVIV 408

Query: 419 VIGSCMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIIAETEQVNLEAGMVNRNQL 478
           VIGSCM+PPD+IF+E  E  +  LSEIRLN+ T +S R+ I+     VNLEAG +NR+ +
Sbjct: 409 VIGSCMSPPDTIFSESGEPTRVELSEIRLNMRTKESNRKVIVTG---VNLEAGHINRSYV 468

Query: 479 GRKTQFAYLALAEPWPKVSGFAKVDLSTGEIRKYLYGEDRYGGEPLFLPREGATAEDDGH 538
           GRK+QF Y+A+A+PWPK SG AKVD+  G + ++ YG  R+GGEP F+P EG   ED G+
Sbjct: 469 GRKSQFVYIAIADPWPKCSGIAKVDIQNGTVSEFNYGPSRFGGEPCFVP-EGEGEEDKGY 528

Query: 539 ILAFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSKDLQKQ 585
           ++ FV DE++ +SE  +V+A  ++  A V+LP RVPYGFHGTF+S   L++Q
Sbjct: 529 VMGFVRDEEKDESEFVVVDATDMKQVAAVRLPERVPYGFHGTFVSENQLKEQ 575

BLAST of Cp4.1LG01g00800 vs. NCBI nr
Match: gi|659131081|ref|XP_008465499.1| (PREDICTED: 9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like [Cucumis melo])

HSP 1 Score: 1054.7 bits (2726), Expect = 6.0e-305
Identity = 527/594 (88.72%), Postives = 550/594 (92.59%), Query Frame = 1

Query: 1   MASSSS-----SSSMP-KILSVNRNYGVRN--RVSCSLHTPSIIQIPKRSQTAFRSPIKI 60
           MASSS      SSSMP K LS   NY  R    VS SLHTPSIIQIPK S T F SP+K 
Sbjct: 1   MASSSFLKSPFSSSMPDKTLS---NYTSRRLLSVSSSLHTPSIIQIPKHSHTTFPSPLKT 60

Query: 61  AVPKPEPVAAPSVSSPAKEWNFLQQAAAMALDAVEDALVSMEKKHSLPKTADPSVQIAGN 120
           ++PKP P++     S +++WNFLQ+AAAMALDAVE+AL+S E+KHSLPKTADP+VQIAGN
Sbjct: 61  SIPKPPPLSTTPPLSGSEDWNFLQRAAAMALDAVENALISAERKHSLPKTADPAVQIAGN 120

Query: 121 FAPVPEQSVRKVLPVTGKVPDCIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFTEGG 180
           FAPVPEQ VRK LPV GKVP+CIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEF+E G
Sbjct: 121 FAPVPEQPVRKGLPVIGKVPECIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFSEAG 180

Query: 181 RVSYACRFTETERLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHSHGIG 240
            VSYACRFTET+RLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDH+ GIG
Sbjct: 181 GVSYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHNQGIG 240

Query: 241 VANAGLVYFNGRLLAMSEDDLPYQIRVTPSGDLKTVGRFDFDGQLKSTMIAHPKLDPVSG 300
           VANAGLVYFNGRLLAMSEDDLPYQIRVTP+GDLKTVGRFDFDGQLKSTMIAHPKLDPVSG
Sbjct: 241 VANAGLVYFNGRLLAMSEDDLPYQIRVTPAGDLKTVGRFDFDGQLKSTMIAHPKLDPVSG 300

Query: 301 EMFALSYDVIQKPYLKYFKFSAEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVFK 360
           EMFALSYDVIQKPYLKYFKFS EGEKSPDVEIPLPQPTMMHDFAITEK+VVIPDQQVVFK
Sbjct: 301 EMFALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKYVVIPDQQVVFK 360

Query: 361 LPEMIRGGSPVVYDKEKTSRFGILDKNATDANAIKWIEAPDCFCFHLWNAWEEPETNEIV 420
           LPEMIRGGSPVVYDKEKTSRFGILDKNA DANAIKWIEAPDCFCFHLWNAWEEPETNEIV
Sbjct: 361 LPEMIRGGSPVVYDKEKTSRFGILDKNAADANAIKWIEAPDCFCFHLWNAWEEPETNEIV 420

Query: 421 VIGSCMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIIAETEQVNLEAGMVNRNQL 480
           VIGSCMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPII+ETEQVNLEAGMVNRN L
Sbjct: 421 VIGSCMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIISETEQVNLEAGMVNRNLL 480

Query: 481 GRKTQFAYLALAEPWPKVSGFAKVDLSTGEIRKYLYGEDRYGGEPLFLPREGATAEDDGH 540
           GRKTQF+YLALAEPWPKVSGFAKVDL TGEI+KYLYGE RYGGEPLFLPREGA AEDDGH
Sbjct: 481 GRKTQFSYLALAEPWPKVSGFAKVDLFTGEIKKYLYGEQRYGGEPLFLPREGAEAEDDGH 540

Query: 541 ILAFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSKDLQKQKR 587
           ILAFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISS+DLQKQ R
Sbjct: 541 ILAFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSEDLQKQIR 591

BLAST of Cp4.1LG01g00800 vs. NCBI nr
Match: gi|449460068|ref|XP_004147768.1| (PREDICTED: 9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like [Cucumis sativus])

HSP 1 Score: 1045.8 bits (2703), Expect = 2.8e-302
Identity = 517/594 (87.04%), Postives = 544/594 (91.58%), Query Frame = 1

Query: 1   MASSSSSS--------SMPKILSVNRNYGVRNRVSCSLHTPSIIQIPKRSQTAFRSPIKI 60
           MASSSSSS        SMP     N        VS SLHTPSIIQIPK S T F SP++ 
Sbjct: 1   MASSSSSSFLQSPFSSSMPDKKLSNYTTNRLLSVSSSLHTPSIIQIPKHSHTTFPSPLQT 60

Query: 61  AVPKPEPVAAPSVSSPAKEWNFLQQAAAMALDAVEDALVSMEKKHSLPKTADPSVQIAGN 120
           ++PKP P+      S +++WNFLQ+AAAMALDAVE+AL+S E+KHSLPKTADP+VQIAGN
Sbjct: 61  SIPKPPPLTTTPPVSGSQDWNFLQRAAAMALDAVENALISAERKHSLPKTADPAVQIAGN 120

Query: 121 FAPVPEQSVRKVLPVTGKVPDCIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFTEGG 180
           FAPVPEQ VRK LPV GKVPD IRGVYVRNGANPLHEP+SGHHLFDGDGMVHAVEF+E G
Sbjct: 121 FAPVPEQPVRKGLPVIGKVPDHIRGVYVRNGANPLHEPLSGHHLFDGDGMVHAVEFSEAG 180

Query: 181 RVSYACRFTETERLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHSHGIG 240
            VSYACRFTET+RLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDH+HGIG
Sbjct: 181 GVSYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHNHGIG 240

Query: 241 VANAGLVYFNGRLLAMSEDDLPYQIRVTPSGDLKTVGRFDFDGQLKSTMIAHPKLDPVSG 300
           VANAGLVYFNGRLLAMSEDDLPYQIRVTP+GDLKTVGRF+FDGQL+STMIAHPKLDPVSG
Sbjct: 241 VANAGLVYFNGRLLAMSEDDLPYQIRVTPAGDLKTVGRFNFDGQLESTMIAHPKLDPVSG 300

Query: 301 EMFALSYDVIQKPYLKYFKFSAEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVFK 360
           EMFALSYDVIQKPYLKYFKFS EGEKSPDVEIPLPQPTMMHDFAITEK+VVIPDQQVVFK
Sbjct: 301 EMFALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKYVVIPDQQVVFK 360

Query: 361 LPEMIRGGSPVVYDKEKTSRFGILDKNATDANAIKWIEAPDCFCFHLWNAWEEPETNEIV 420
           LPEMIRGGSPVVYDKEKTSRFGILDKNATDANAIKWIEAPDCFCFHLWNAWEEPETNE+V
Sbjct: 361 LPEMIRGGSPVVYDKEKTSRFGILDKNATDANAIKWIEAPDCFCFHLWNAWEEPETNEVV 420

Query: 421 VIGSCMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIIAETEQVNLEAGMVNRNQL 480
           VIGSCMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPII ETEQVNLEAGMVNRN L
Sbjct: 421 VIGSCMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIITETEQVNLEAGMVNRNLL 480

Query: 481 GRKTQFAYLALAEPWPKVSGFAKVDLSTGEIRKYLYGEDRYGGEPLFLPREGATAEDDGH 540
           GRKTQF+YLALAEPWPKVSGFAKVD+ +GE++KYLYGE RYGGEPLFLPREGA AEDDGH
Sbjct: 481 GRKTQFSYLALAEPWPKVSGFAKVDVLSGEVKKYLYGEQRYGGEPLFLPREGAEAEDDGH 540

Query: 541 ILAFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSKDLQKQKR 587
           ILAFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFIS KDLQKQ R
Sbjct: 541 ILAFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISCKDLQKQIR 594

BLAST of Cp4.1LG01g00800 vs. NCBI nr
Match: gi|408794955|gb|AFU91491.1| (9-cis-epoxycarotenoid dioxygenase [Momordica charantia])

HSP 1 Score: 1041.2 bits (2691), Expect = 6.9e-301
Identity = 505/581 (86.92%), Postives = 543/581 (93.46%), Query Frame = 1

Query: 4   SSSSSSMPKILSVNRNYGVRNRVSCSLHTPSIIQIPKRSQTAFRSPIKIAVPKPEPVAAP 63
           S  S SMP++LSVN NY V+NRVSCSLHTPSI+QIPK SQT    P+K  V KP    +P
Sbjct: 12  SGFSPSMPELLSVNSNY-VKNRVSCSLHTPSIVQIPKHSQTFRPPPLKTPVEKP---VSP 71

Query: 64  SVSSPAKEWNFLQQAAAMALDAVEDALVSMEKKHSLPKTADPSVQIAGNFAPVPEQSVRK 123
           S S+P ++WNFLQ+AA+MA+DAVE+AL+S E+K  LPKTADP VQIAGNFAPVPEQ VRK
Sbjct: 72  STSAP-EDWNFLQRAASMAIDAVENALLSAERKRPLPKTADPEVQIAGNFAPVPEQPVRK 131

Query: 124 VLPVTGKVPDCIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFTEGGRVSYACRFTET 183
            LPVTG++P+CIRGVYVRNGANPLHEP +GHHLFDGDGMVHAVEF++GG VSYACRFTET
Sbjct: 132 ALPVTGRIPECIRGVYVRNGANPLHEPAAGHHLFDGDGMVHAVEFSDGGGVSYACRFTET 191

Query: 184 ERLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHSHGIGVANAGLVYFNG 243
           +RLVQERA+GRPVFPKAIGELHGHSGIARL+LFYARGLFGLVDHSHGIGVANAGLVYFNG
Sbjct: 192 QRLVQERAHGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHGIGVANAGLVYFNG 251

Query: 244 RLLAMSEDDLPYQIRVTPSGDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEMFALSYDVIQ 303
           RLLAMSEDDLPYQIRVTP+GDLKT+GRFDFDGQLKSTMIAHPKLDPVSGE+FA+SYDVIQ
Sbjct: 252 RLLAMSEDDLPYQIRVTPAGDLKTIGRFDFDGQLKSTMIAHPKLDPVSGELFAISYDVIQ 311

Query: 304 KPYLKYFKFSAEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVFKLPEMIRGGSPV 363
           KP+LKYFKFS EGEKSPDVE+PLPQPTMMHDFAITEKFVVIPDQQVVFKLPEMIRGGSPV
Sbjct: 312 KPHLKYFKFSPEGEKSPDVEVPLPQPTMMHDFAITEKFVVIPDQQVVFKLPEMIRGGSPV 371

Query: 364 VYDKEKTSRFGILDKNATDANAIKWIEAPDCFCFHLWNAWEEPETNEIVVIGSCMTPPDS 423
           +YDK K SRFGIL KNATDA  IKWIEAPDCFCFHLWNAWEEPETNE+VVIGSCMTPPDS
Sbjct: 372 IYDKNKISRFGILPKNATDAGDIKWIEAPDCFCFHLWNAWEEPETNEVVVIGSCMTPPDS 431

Query: 424 IFNECEENLKSVLSEIRLNLSTGKSTRRPIIAETEQVNLEAGMVNRNQLGRKTQFAYLAL 483
           IFNECEENLKSVLSEIRLNL+TGKSTRRPII ETEQVNLEAGMVNRN+LGRKTQFAYLAL
Sbjct: 432 IFNECEENLKSVLSEIRLNLTTGKSTRRPIIPETEQVNLEAGMVNRNRLGRKTQFAYLAL 491

Query: 484 AEPWPKVSGFAKVDLSTGEIRKYLYGEDRYGGEPLFLPREGATAEDDGHILAFVHDEKEW 543
           AEPWPKVSGFAKVDLSTGEIRKY+YGE RYGGEPLFLPREGA AEDDGHILAFVHDEKEW
Sbjct: 492 AEPWPKVSGFAKVDLSTGEIRKYIYGEQRYGGEPLFLPREGAEAEDDGHILAFVHDEKEW 551

Query: 544 KSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSKDLQKQ 585
           KSELQIVNA+TL+LEA+VKLPSRVPYGFHGTFI SK LQKQ
Sbjct: 552 KSELQIVNAITLKLEASVKLPSRVPYGFHGTFIGSKGLQKQ 587

BLAST of Cp4.1LG01g00800 vs. NCBI nr
Match: gi|1009109982|ref|XP_015893126.1| (PREDICTED: 9-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic-like [Ziziphus jujuba])

HSP 1 Score: 934.9 bits (2415), Expect = 7.0e-269
Identity = 454/584 (77.74%), Postives = 511/584 (87.50%), Query Frame = 1

Query: 3   SSSSSSSMPKILSVNRNYGVRNRVSCSLHTPSIIQIPKRSQTAFRSPIKIAVP-KPEPVA 62
           S SSSSS P  L +      R  ++CSL TPS+I+IPK+S + ++      +  K  P +
Sbjct: 6   SCSSSSSSP-FLELGNGKKRRPNINCSLQTPSVIRIPKQSPSTYQPSNTTTIKEKSTPNS 65

Query: 63  APSVSSPAKEWNFLQQAAAMALDAVEDALVSMEKKHSLPKTADPSVQIAGNFAPVPEQSV 122
                 P ++WNFLQ+AAAMAL AVE ALV  E+KH LPKTADPSVQIAGNFAPV EQ V
Sbjct: 66  VSDKPVPFQQWNFLQKAAAMALQAVESALVEHERKHPLPKTADPSVQIAGNFAPVQEQPV 125

Query: 123 RKVLPVTGKVPDCIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFTEGGRVSYACRFT 182
           +  LPVTGK+P CI GVYVRNGANP +EPV+GHH FDGDGMVHAV+F + G  SY+CRFT
Sbjct: 126 QHSLPVTGKIPKCIEGVYVRNGANPHYEPVAGHHFFDGDGMVHAVQF-KNGTASYSCRFT 185

Query: 183 ETERLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHSHGIGVANAGLVYF 242
           ET RLVQERA+GRPVFPKAIGELHGHSGIARL+LFYARG+FGLVD +HG+GVANAGLVYF
Sbjct: 186 ETHRLVQERAFGRPVFPKAIGELHGHSGIARLLLFYARGIFGLVDSNHGMGVANAGLVYF 245

Query: 243 NGRLLAMSEDDLPYQIRVTPSGDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEMFALSYDV 302
           NGRLLAMSEDDLPY +R+TPSGDLKTVGR+DF+GQLKSTMIAHPKLDP +GE+FALSYDV
Sbjct: 246 NGRLLAMSEDDLPYHVRITPSGDLKTVGRYDFNGQLKSTMIAHPKLDPETGELFALSYDV 305

Query: 303 IQKPYLKYFKFSAEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVFKLPEMIRGGS 362
           +Q+PYLKYF FS  G+KS DVEIPL QPTMMHDFAITE FVVIPDQQVVFKLPEMIRGGS
Sbjct: 306 VQRPYLKYFHFSPSGKKSTDVEIPLSQPTMMHDFAITENFVVIPDQQVVFKLPEMIRGGS 365

Query: 363 PVVYDKEKTSRFGILDKNATDANAIKWIEAPDCFCFHLWNAWEEPETNEIVVIGSCMTPP 422
           PV+YD+EK +RFGILDKNATDA+ IKWIEAPDCFCFHLWNAWEEPE +E+VVIGSCMTPP
Sbjct: 366 PVIYDREKMARFGILDKNATDASGIKWIEAPDCFCFHLWNAWEEPENDEVVVIGSCMTPP 425

Query: 423 DSIFNECEENLKSVLSEIRLNLSTGKSTRRPIIAETEQVNLEAGMVNRNQLGRKTQFAYL 482
           DSIFNECEENLKSVLSEIRLNL TGKSTRRPII+E EQVNLEAGMVNRN+LGRKT+FAYL
Sbjct: 426 DSIFNECEENLKSVLSEIRLNLKTGKSTRRPIISEQEQVNLEAGMVNRNRLGRKTRFAYL 485

Query: 483 ALAEPWPKVSGFAKVDLSTGEIRKYLYGEDRYGGEPLFLPRE-GATAEDDGHILAFVHDE 542
           ALAEPWPKVSGFAKVDL TGE+ K++YGE R+GGEPLFLP++  +  EDDG+ILAFVHDE
Sbjct: 486 ALAEPWPKVSGFAKVDLFTGEVNKFMYGEQRFGGEPLFLPKDPNSENEDDGYILAFVHDE 545

Query: 543 KEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSKDLQKQ 585
           KEWKSELQIVNAMT++LEAT+KLPSRVPYGFHGTFISSKDL+KQ
Sbjct: 546 KEWKSELQIVNAMTMKLEATIKLPSRVPYGFHGTFISSKDLEKQ 587

BLAST of Cp4.1LG01g00800 vs. NCBI nr
Match: gi|147856958|emb|CAN81383.1| (hypothetical protein VITISV_018449 [Vitis vinifera])

HSP 1 Score: 932.2 bits (2408), Expect = 4.5e-268
Identity = 452/589 (76.74%), Postives = 517/589 (87.78%), Query Frame = 1

Query: 2   ASSSSSSSMPKILSVN----RNYGVRNRVSCSLHTPSIIQIPKRSQTAFR-SPIKIAVPK 61
           +SSSSSSS    L +         +R  ++CSLH+PS++  PK + ++   + +K  +PK
Sbjct: 25  SSSSSSSSSSSALDLGSFSFHRPTIRPNINCSLHSPSVLHFPKHTSSSTGVATVKTTLPK 84

Query: 62  PEPVAAPSVSSPAKEWNFLQQAAAMALDAVEDALVSMEKKHSLPKTADPSVQIAGNFAPV 121
            +   + SVS   ++WNFLQ AAAMALDAVE ALV+ E +H LPKTADP VQIAGNFAPV
Sbjct: 85  EKDDFSSSVS---QQWNFLQSAAAMALDAVETALVARETQHPLPKTADPRVQIAGNFAPV 144

Query: 122 PEQSVRKVLPVTGKVPDCIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFTEGGRVSY 181
           PEQ V+  LPV G++PDCI+GVY RNGANPLH+PV+GHHLFDGDGMVHAV+F +G   SY
Sbjct: 145 PEQPVQHSLPVDGRIPDCIQGVYTRNGANPLHKPVAGHHLFDGDGMVHAVQFKDGD-ASY 204

Query: 182 ACRFTETERLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHSHGIGVANA 241
           ACRFTET+RL+QER +GRPVFPKAIGELHGHSGIARL+LFYARGLFGLVDHSHG GVANA
Sbjct: 205 ACRFTETQRLIQERDFGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHGTGVANA 264

Query: 242 GLVYFNGRLLAMSEDDLPYQIRVTPSGDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEMFA 301
           GLVYFN RLLAMSEDDLPYQIRVTPSGDL+TVGR+DF+GQL+STMIAHPK+DPVSGEMFA
Sbjct: 265 GLVYFNRRLLAMSEDDLPYQIRVTPSGDLETVGRYDFEGQLRSTMIAHPKVDPVSGEMFA 324

Query: 302 LSYDVIQKPYLKYFKFSAEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVFKLPEM 361
           LSYDV+QKPYLKYF+FS +G+KSPDVEIPL +PTMMHDFAITE+FVV+P+QQVVFKL EM
Sbjct: 325 LSYDVVQKPYLKYFRFSPKGDKSPDVEIPLAEPTMMHDFAITERFVVVPNQQVVFKLQEM 384

Query: 362 IRGGSPVVYDKEKTSRFGILDKNATDANAIKWIEAPDCFCFHLWNAWEEPETNEIVVIGS 421
           I GGSPVVYDK K SRFG+LDKNATDA+ + WIEAPDCFCFHLWNAWEEPET+E+VVIGS
Sbjct: 385 ISGGSPVVYDKNKMSRFGVLDKNATDASGMXWIEAPDCFCFHLWNAWEEPETDEVVVIGS 444

Query: 422 CMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIIAETEQVNLEAGMVNRNQLGRKT 481
           CMTPPDSIFNEC+E LKSVLSEIRLNL TGKSTRRPI+ E+EQVNLEAGMVNRN+LGRKT
Sbjct: 445 CMTPPDSIFNECDEGLKSVLSEIRLNLKTGKSTRRPILPESEQVNLEAGMVNRNRLGRKT 504

Query: 482 QFAYLALAEPWPKVSGFAKVDLSTGEIRKYLYGEDRYGGEPLFLPRE-GATAEDDGHILA 541
           QFAYLA+AEPWPKVSGFAKVDLSTG++ KY+YGE RYGGEPLFLPR+  +  EDDG+ILA
Sbjct: 505 QFAYLAVAEPWPKVSGFAKVDLSTGQVSKYIYGEQRYGGEPLFLPRDPNSGREDDGYILA 564

Query: 542 FVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSKDLQKQ 585
           FVHDEK WKSELQIVNA  L+LEA+VKLPSRVPYGFHGTFISSK+L+KQ
Sbjct: 565 FVHDEKTWKSELQIVNATNLQLEASVKLPSRVPYGFHGTFISSKELEKQ 609

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
NCED3_ARATH1.1e-25374.419-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic OS=Arabidopsis thaliana G... [more]
NCED1_PHAVU2.4e-25373.149-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic OS=Phaseolus vulgaris GN=... [more]
NCED9_ARATH1.0e-24070.689-cis-epoxycarotenoid dioxygenase NCED9, chloroplastic OS=Arabidopsis thaliana G... [more]
NCED5_ARATH1.1e-23273.07Probable 9-cis-epoxycarotenoid dioxygenase NCED5, chloroplastic OS=Arabidopsis t... [more]
NCED_ONCHC4.4e-23169.249-cis-epoxycarotenoid dioxygenase, chloroplastic OS=Oncidium hybrid cultivar GN=... [more]
Match NameE-valueIdentityDescription
K4KBB8_MOMCH4.8e-30186.929-cis-epoxycarotenoid dioxygenase OS=Momordica charantia GN=NCED PE=2 SV=1[more]
F6HR90_VITVI3.2e-26877.13Putative uncharacterized protein OS=Vitis vinifera GN=VIT_19s0093g00550 PE=4 SV=... [more]
A5BR72_VITVI3.2e-26876.74Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_018449 PE=4 SV=1[more]
I7FMA3_VITVI3.2e-26876.749-cis epoxy carotenoid dioxygenase 3 OS=Vitis vinifera GN=NCED3 PE=2 SV=1[more]
Q5SGD1_VITVI4.1e-26876.749-cis-epoxycarotenoid dioxygenase 1 OS=Vitis vinifera GN=NCED1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AT3G14440.16.1e-25574.41 nine-cis-epoxycarotenoid dioxygenase 3[more]
AT1G78390.15.9e-24270.68 nine-cis-epoxycarotenoid dioxygenase 9[more]
AT1G30100.15.9e-23473.07 nine-cis-epoxycarotenoid dioxygenase 5[more]
AT4G18350.12.6e-22169.49 nine-cis-epoxycarotenoid dioxygenase 2[more]
AT3G24220.11.6e-18357.71 nine-cis-epoxycarotenoid dioxygenase 6[more]
Match NameE-valueIdentityDescription
gi|659131081|ref|XP_008465499.1|6.0e-30588.72PREDICTED: 9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like [Cucumis ... [more]
gi|449460068|ref|XP_004147768.1|2.8e-30287.04PREDICTED: 9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like [Cucumis ... [more]
gi|408794955|gb|AFU91491.1|6.9e-30186.929-cis-epoxycarotenoid dioxygenase [Momordica charantia][more]
gi|1009109982|ref|XP_015893126.1|7.0e-26977.74PREDICTED: 9-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic-like [Ziziphus... [more]
gi|147856958|emb|CAN81383.1|4.5e-26876.74hypothetical protein VITISV_018449 [Vitis vinifera][more]
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
Vocabulary: Molecular Function
TermDefinition
GO:0016702oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen
Vocabulary: INTERPRO
TermDefinition
IPR004294Carotenoid_Oase
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0042572 retinol metabolic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003834 beta-carotene 15,15'-monooxygenase activity
molecular_function GO:0016702 oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen
molecular_function GO:0046872 metal ion binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG01g00800.1Cp4.1LG01g00800.1mRNA


Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004294Carotenoid oxygenasePANTHERPTHR10543BETA-CAROTENE DIOXYGENASEcoord: 62..584
score:
IPR004294Carotenoid oxygenasePFAMPF03055RPE65coord: 114..576
score: 3.2E
NoneNo IPR availablePANTHERPTHR10543:SF429-CIS-EPOXYCAROTENOID DIOXYGENASE NCED2, CHLOROPLASTIC-RELATEDcoord: 62..584
score: