CSPI04G07560 (gene) Wild cucumber (PI 183967)

BLAST of CSPI04G07560 vs. Swiss-Prot
Match: NCED3_ARATH (9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic OS=Arabidopsis thaliana GN=NCED3 PE=2 SV=1)

HSP 1 Score: 871.7 bits (2251), Expect = 4.6e-252
Identity = 438/593 (73.86%), Postives = 496/593 (83.64%), Query Frame = 1

Query: 1   MASSSSSFLQSPFSSSMPDKKLSNYTTNSLLSVSSSLHTPSIIQIPKHSHTTFPSPLQTS 60
           ++SS SS L   + SS+P   +++  T  L +VSS+LHTP  +  PK S     SP    
Sbjct: 24  LSSSQSSDLS--YCSSLP---MASRVTRKL-NVSSALHTPPALHFPKQSSN---SPAIVV 83

Query: 61  IPKPPPLTTTPPVSGSQDWNFLQRAAAMALDAVENALISAERKHSLPKTADPAVQIAGNF 120
            PK          S ++  N  QRAAA ALDA E  L+S E+ H LPKTADP+VQIAGNF
Sbjct: 84  KPKAKE-------SNTKQMNLFQRAAAAALDAAEGFLVSHEKLHPLPKTADPSVQIAGNF 143

Query: 121 APVPEQPVRKGLPVIGKVPDHIRGVYVRNGANPLHEPLSGHHLFDGDGMVHAVEFSEAGG 180
           APV EQPVR+ LPV+GK+PD I+GVYVRNGANPLHEP++GHH FDGDGMVHAV+F E G 
Sbjct: 144 APVNEQPVRRNLPVVGKLPDSIKGVYVRNGANPLHEPVTGHHFFDGDGMVHAVKF-EHGS 203

Query: 181 VSYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHNHGIGV 240
            SYACRFT+T R VQER  GRPVFPKAIGELHGH+GIARLMLFYAR   G+VD  HG GV
Sbjct: 204 ASYACRFTQTNRFVQERQLGRPVFPKAIGELHGHTGIARLMLFYARAAAGIVDPAHGTGV 263

Query: 241 ANAGLVYFNGRLLAMSEDDLPYQIRVTPAGDLKTVGRFNFDGQLESTMIAHPKLDPVSGE 300
           ANAGLVYFNGRLLAMSEDDLPYQ+++TP GDLKTVGRF+FDGQLESTMIAHPK+DP SGE
Sbjct: 264 ANAGLVYFNGRLLAMSEDDLPYQVQITPNGDLKTVGRFDFDGQLESTMIAHPKVDPESGE 323

Query: 301 MFALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKYVVIPDQQVVFKL 360
           +FALSYDV+ KPYLKYF+FSP+G KSPDVEI L QPTMMHDFAITE +VV+PDQQVVFKL
Sbjct: 324 LFALSYDVVSKPYLKYFRFSPDGTKSPDVEIQLDQPTMMHDFAITENFVVVPDQQVVFKL 383

Query: 361 PEMIRGGSPVVYDKEKTSRFGILDKNATDANAIKWIEAPDCFCFHLWNAWEEPETNEVVV 420
           PEMIRGGSPVVYDK K +RFGILDK A D++ IKWI+APDCFCFHLWNAWEEPET+EVVV
Sbjct: 384 PEMIRGGSPVVYDKNKVARFGILDKYAEDSSNIKWIDAPDCFCFHLWNAWEEPETDEVVV 443

Query: 421 IGSCMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIIT-ETEQVNLEAGMVNRNLL 480
           IGSCMTPPDSIFNE +ENLKSVLSEIRLNL TG+STRRPII+ E +QVNLEAGMVNRN+L
Sbjct: 444 IGSCMTPPDSIFNESDENLKSVLSEIRLNLKTGESTRRPIISNEDQQVNLEAGMVNRNML 503

Query: 481 GRKTQFSYLALAEPWPKVSGFAKVDVLSGEVKKYLYGEQSYGGEPLFLPREGAEAEDDGH 540
           GRKT+F+YLALAEPWPKVSGFAKVD+ +GEVKK+LYG+  YGGEPLFLP EG E ED+G+
Sbjct: 504 GRKTKFAYLALAEPWPKVSGFAKVDLTTGEVKKHLYGDNRYGGEPLFLPGEGGE-EDEGY 563

Query: 541 ILAFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISCKDLQKQI 593
           IL FVHDEK WKSELQIVNA++LE+EATVKLPSRVPYGFHGTFI   DL KQ+
Sbjct: 564 ILCFVHDEKTWKSELQIVNAVSLEVEATVKLPSRVPYGFHGTFIGADDLAKQV 598

BLAST of CSPI04G07560 vs. Swiss-Prot
Match: NCED1_PHAVU (9-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic OS=Phaseolus vulgaris GN=NCED1 PE=2 SV=1)

HSP 1 Score: 867.1 bits (2239), Expect = 1.1e-250
Identity = 431/598 (72.07%), Postives = 496/598 (82.94%), Query Frame = 1

Query: 1   MASSSSSFLQSPFSSSMPDKKLSNYTTNSLLSVSSSLHTPSIIQIPKHSHTTFPSPLQ-- 60
           +AS+SSS      ++ +P KK S+  TN++     +LH P   Q    S TT P+P++  
Sbjct: 25  LASTSSSP-----TTLLPFKKRSSSNTNTITCSLQTLHYPKQYQPTSTSTTTTPTPIKPT 84

Query: 61  TSIPKPPPLTTTPPVSGS-----QDWNFLQRAAAMALDAVENALISAERKHSLPKTADPA 120
           T+     P   T P+S +     Q WNFLQ+AAA  LD VE AL+S E KH LPKTADP 
Sbjct: 85  TTTTTTTPHRETKPLSDTKQPFPQKWNFLQKAAATGLDMVETALVSHESKHPLPKTADPK 144

Query: 121 VQIAGNFAPVPEQPVRKGLPVIGKVPDHIRGVYVRNGANPLHEPLSGHHLFDGDGMVHAV 180
           VQIAGNFAPVPE    + LPV+GK+P  I GVYVRNGANPL+EP++GHH FDGDGMVHAV
Sbjct: 145 VQIAGNFAPVPEHAADQALPVVGKIPKCIDGVYVRNGANPLYEPVAGHHFFDGDGMVHAV 204

Query: 181 EFSEAGGVSYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVD 240
           +F+  G  SYACRFTETQRL QE++ GRPVFPKAIGELHGHSGIARL+LFYAR LF LVD
Sbjct: 205 KFTN-GAASYACRFTETQRLAQEKSLGRPVFPKAIGELHGHSGIARLLLFYARSLFQLVD 264

Query: 241 HNHGIGVANAGLVYFNGRLLAMSEDDLPYQIRVTPAGDLKTVGRFNFDGQLESTMIAHPK 300
            +HG+GVANAGLVYFN  LLAMSEDDLPY +R+T  GDL TVGR++F+GQL STMIAHPK
Sbjct: 265 GSHGMGVANAGLVYFNNHLLAMSEDDLPYHVRITSNGDLTTVGRYDFNGQLNSTMIAHPK 324

Query: 301 LDPVSGEMFALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKYVVIPD 360
           LDPV+G++ ALSYDV+QKPYLKYF+FS +G KSPDVEIPL +PTMMHDFAITE +VV+PD
Sbjct: 325 LDPVNGDLHALSYDVVQKPYLKYFRFSADGVKSPDVEIPLKEPTMMHDFAITENFVVVPD 384

Query: 361 QQVVFKLPEMIRGGSPVVYDKEKTSRFGILDKNATDANAIKWIEAPDCFCFHLWNAWEEP 420
           QQVVFKL EMI GGSPVVYDK KTSRFGILDKNA DANA++WI+AP+CFCFHLWNAWEEP
Sbjct: 385 QQVVFKLTEMITGGSPVVYDKNKTSRFGILDKNAKDANAMRWIDAPECFCFHLWNAWEEP 444

Query: 421 ETNEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIITETEQVNLEAGM 480
           ET+E+VVIGSCMTP DSIFNEC+E+LKSVLSEIRLNL TGKSTRRPII++ EQVNLEAGM
Sbjct: 445 ETDEIVVIGSCMTPADSIFNECDESLKSVLSEIRLNLRTGKSTRRPIISDAEQVNLEAGM 504

Query: 481 VNRNLLGRKTQFSYLALAEPWPKVSGFAKVDVLSGEVKKYLYGEQSYGGEPLFLPREGAE 540
           VNRN LGRKTQF+YLALAEPWPKVSGFAKVD+ SGEV+KY+YGE+ +GGEPLFLP    E
Sbjct: 505 VNRNKLGRKTQFAYLALAEPWPKVSGFAKVDLFSGEVQKYMYGEEKFGGEPLFLP--NGE 564

Query: 541 AEDDGHILAFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISCKDLQKQ 592
            E DG+ILAFVHDEKEWKSELQIVNA  L+LEA++KLPSRVPYGFHGTFI  KDL+KQ
Sbjct: 565 EEGDGYILAFVHDEKEWKSELQIVNAQNLKLEASIKLPSRVPYGFHGTFIHSKDLRKQ 614

BLAST of CSPI04G07560 vs. Swiss-Prot
Match: NCED9_ARATH (9-cis-epoxycarotenoid dioxygenase NCED9, chloroplastic OS=Arabidopsis thaliana GN=NCED9 PE=2 SV=1)

HSP 1 Score: 813.9 bits (2101), Expect = 1.1e-234
Identity = 415/603 (68.82%), Postives = 489/603 (81.09%), Query Frame = 1

Query: 1   MASSSSSFLQSPFSSSMPDK--KLSNYTTNSLLSVSSSLHTPSIIQIPKHSHTTFPSPLQ 60
           + S+S+ FL   FS+S      KL + + +S L  +  L  P  +    +  ++  S LQ
Sbjct: 57  LPSTSTQFLDRTFSTSSSSSRPKLQSLSFSSTLR-NKKLVVPCYVSSSVNKKSSVSSSLQ 116

Query: 61  TSIPKPPP-------LTTTPPVSGSQD--WNFLQRAAAMALDAVENALISAERK-HSLPK 120
           +   KPP        +T   P + +Q+   N +QR AAM LDAVENA+IS ER+ H  PK
Sbjct: 117 SPTFKPPSWKKLCNDVTNLIPKTTNQNPKLNPVQRTAAMVLDAVENAMISHERRRHPHPK 176

Query: 121 TADPAVQIAGNFAPVPEQPVRKGLPVIGKVPDHIRGVYVRNGANPLHEPLSGHHLFDGDG 180
           TADPAVQIAGNF PVPE+PV   LPV G VP+ I+GVYVRNGANPLH+P+SGHHLFDGDG
Sbjct: 177 TADPAVQIAGNFFPVPEKPVVHNLPVTGTVPECIQGVYVRNGANPLHKPVSGHHLFDGDG 236

Query: 181 MVHAVEFSEAGGVSYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGL 240
           MVHAV F   G VSYACRFTET RLVQER  GRPVFPKAIGELHGH GIA+LMLF  RGL
Sbjct: 237 MVHAVRFDN-GSVSYACRFTETNRLVQERECGRPVFPKAIGELHGHLGIAKLMLFNTRGL 296

Query: 241 FGLVDHNHGIGVANAGLVYFNGRLLAMSEDDLPYQIRVTPAGDLKTVGRFNFDGQLESTM 300
           FGLVD   G+GVANAGLVYFNG LLAMSEDDLPY ++VT  GDL+T GR++FDGQL+STM
Sbjct: 297 FGLVDPTGGLGVANAGLVYFNGHLLAMSEDDLPYHVKVTQTGDLETSGRYDFDGQLKSTM 356

Query: 301 IAHPKLDPVSGEMFALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKY 360
           IAHPK+DP + E+FALSYDV+ KPYLKYF+F+ +GEKSPDVEIPL QPTM+HDFAITE +
Sbjct: 357 IAHPKIDPETRELFALSYDVVSKPYLKYFRFTSDGEKSPDVEIPLDQPTMIHDFAITENF 416

Query: 361 VVIPDQQVVFKLPEMIRGGSPVVYDKEKTSRFGILDKNATDANAIKWIEAPDCFCFHLWN 420
           VVIPDQQVVF+LPEMIRGGSPVVYD++K SRFGIL+KNA DA++I+WIE PDCFCFHLWN
Sbjct: 417 VVIPDQQVVFRLPEMIRGGSPVVYDEKKKSRFGILNKNAKDASSIQWIEVPDCFCFHLWN 476

Query: 421 AWEEPETNEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIITETEQVN 480
           +WEEPET+EVVVIGSCMTPPDSIFNE +E L+SVLSEIRLNL TG+STRRP+I  +EQVN
Sbjct: 477 SWEEPETDEVVVIGSCMTPPDSIFNEHDETLQSVLSEIRLNLKTGESTRRPVI--SEQVN 536

Query: 481 LEAGMVNRNLLGRKTQFSYLALAEPWPKVSGFAKVDVLSGEVKKYLYGEQSYGGEPLFLP 540
           LEAGMVNRNLLGRKT+++YLAL EPWPKVSGFAKVD+ +GE++KY+YGE  YGGEPLFLP
Sbjct: 537 LEAGMVNRNLLGRKTRYAYLALTEPWPKVSGFAKVDLSTGEIRKYIYGEGKYGGEPLFLP 596

Query: 541 REGAEAEDDGHILAFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISCKDL 592
             G   ED G+I+ FVHDE++ KSELQ++NA+ ++LEATV LPSRVPYGFHGTFIS +DL
Sbjct: 597 -SGDGEEDGGYIMVFVHDEEKVKSELQLINAVNMKLEATVTLPSRVPYGFHGTFISKEDL 654

BLAST of CSPI04G07560 vs. Swiss-Prot
Match: NCED5_ARATH (Probable 9-cis-epoxycarotenoid dioxygenase NCED5, chloroplastic OS=Arabidopsis thaliana GN=NCED5 PE=1 SV=1)

HSP 1 Score: 796.2 bits (2055), Expect = 2.5e-229
Identity = 395/589 (67.06%), Postives = 462/589 (78.44%), Query Frame = 1

Query: 7   SFLQSPFSSSMPDKKLSNYTTNSL---LSVSSSLHTPSIIQIPKHSHTTFPSPLQTSIPK 66
           SF  S   +  P   +S   T      LS +S   TP+++  P +             P 
Sbjct: 17  SFAPSDLDAPSPSSSVSFTNTKPRRRKLSANSVSDTPNLLNFPNY-------------PS 76

Query: 67  PPPLTTTPPVSGSQDWNFLQRAAAMALDAVENALISAERKHSLPKTADPAVQIAGNFAPV 126
           P P+    P   +  WN LQRAA+ ALD  E AL+  ER   LPKT DP  QI+GN+APV
Sbjct: 77  PNPII---PEKDTSRWNPLQRAASAALDFAETALLRRERSKPLPKTVDPRHQISGNYAPV 136

Query: 127 PEQPVRKGLPVIGKVPDHIRGVYVRNGANPLHEPLSGHHLFDGDGMVHAVEFSEAGGVSY 186
           PEQ V+  L V GK+PD I GVY+RNGANPL EP+SGHHLFDGDGMVHAV+ +  G  SY
Sbjct: 137 PEQSVKSSLSVDGKIPDCIDGVYLRNGANPLFEPVSGHHLFDGDGMVHAVKITN-GDASY 196

Query: 187 ACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHNHGIGVANA 246
           +CRFTET+RLVQE+  G P+FPKAIGELHGHSGIARLMLFYARGLFGL++H +G GVANA
Sbjct: 197 SCRFTETERLVQEKQLGSPIFPKAIGELHGHSGIARLMLFYARGLFGLLNHKNGTGVANA 256

Query: 247 GLVYFNGRLLAMSEDDLPYQIRVTPAGDLKTVGRFNFDGQLESTMIAHPKLDPVSGEMFA 306
           GLVYF+ RLLAMSEDDLPYQ+RVT  GDL+T+GRF+FDGQL S MIAHPK+DPV+ E+FA
Sbjct: 257 GLVYFHDRLLAMSEDDLPYQVRVTDNGDLETIGRFDFDGQLSSAMIAHPKIDPVTKELFA 316

Query: 307 LSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKYVVIPDQQVVFKLPEM 366
           LSYDV++KPYLKYFKFSPEGEKSPDVEIPL  PTMMHDFAITE +VVIPDQQVVFKL +M
Sbjct: 317 LSYDVVKKPYLKYFKFSPEGEKSPDVEIPLASPTMMHDFAITENFVVIPDQQVVFKLSDM 376

Query: 367 IRGGSPVVYDKEKTSRFGILDKNATDANAIKWIEAPDCFCFHLWNAWEEPETNEVVVIGS 426
             G SPV YD EK SRFGIL +NA DA+ + W+E+P+ FCFHLWNAWE PET+EVVVIGS
Sbjct: 377 FLGKSPVKYDGEKISRFGILPRNAKDASEMVWVESPETFCFHLWNAWESPETDEVVVIGS 436

Query: 427 CMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIITETEQVNLEAGMVNRNLLGRKT 486
           CMTP DSIFNEC+E L SVLSEIRLNL TGKSTRR II  + Q+NLEAGMVNRNLLGRKT
Sbjct: 437 CMTPADSIFNECDEQLNSVLSEIRLNLKTGKSTRRTIIPGSVQMNLEAGMVNRNLLGRKT 496

Query: 487 QFSYLALAEPWPKVSGFAKVDVLSGEVKKYLYGEQSYGGEPLFLPRE-GAEAEDDGHILA 546
           +++YLA+AEPWPKVSGFAKVD+ +GEVK + YG + YGGEP FLPR   ++ EDDG+I++
Sbjct: 497 RYAYLAIAEPWPKVSGFAKVDLSTGEVKNHFYGGKKYGGEPFFLPRGLESDGEDDGYIMS 556

Query: 547 FVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISCKDLQKQ 592
           FVHDE+ W+SEL IVNA+TLELEATVKLPSRVPYGFHGTF++  D+  Q
Sbjct: 557 FVHDEESWESELHIVNAVTLELEATVKLPSRVPYGFHGTFVNSADMLNQ 588

BLAST of CSPI04G07560 vs. Swiss-Prot
Match: NCED_ONCHC (9-cis-epoxycarotenoid dioxygenase, chloroplastic OS=Oncidium hybrid cultivar GN=NCED PE=2 SV=1)

HSP 1 Score: 786.9 bits (2031), Expect = 1.5e-226
Identity = 386/549 (70.31%), Postives = 443/549 (80.69%), Query Frame = 1

Query: 51  TTFPSPLQTSIPKPP--PLTTTPPVSGSQD---WNFLQRAAAMALDAVENALIS--AERK 110
           TT PSP     P PP  P    P  S       WN  QRAAA  L+AVE+ LI    E  
Sbjct: 65  TTSPSPFY---PLPPTCPKEIHPEQSAKPSRPSWNLFQRAAAAVLEAVEDNLIQNLLESG 124

Query: 111 HSLPKTADPAVQIAGNFAPVPEQPVRKGLPVIGKVPDHIRGVYVRNGANPLHEPLSGHHL 170
           H LPKTADPAVQIAGNFAPV EQ     LPV G++P  I GVY+RNGANPL EP++GHH 
Sbjct: 125 HPLPKTADPAVQIAGNFAPVGEQKPHHDLPVDGRIPPLINGVYLRNGANPLFEPVAGHHF 184

Query: 171 FDGDGMVHAVEFSEAGGVSYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLF 230
           FDGDGMVHAV     G  SYACRFTET+RL QERA GR +FPKAIGELHGHSGIARL+LF
Sbjct: 185 FDGDGMVHAVHLRN-GRASYACRFTETERLKQERAVGRAIFPKAIGELHGHSGIARLLLF 244

Query: 231 YARGLFGLVDHNHGIGVANAGLVYFNGRLLAMSEDDLPYQIRVTPAGDLKTVGRFNFDGQ 290
           YARGL GL+DH+ G GVANAGL+YFN RLLAMSEDDLPY +R+ P GDL+T GR++FDGQ
Sbjct: 245 YARGLLGLIDHSRGTGVANAGLIYFNNRLLAMSEDDLPYHVRIKPNGDLETAGRYDFDGQ 304

Query: 291 LESTMIAHPKLDPVSGEMFALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFA 350
           L +TMIAHPKLDP + E FALSYDVI+KPYLKYF+FSP GEKSPDVEIPLPQPTMMHDFA
Sbjct: 305 LTTTMIAHPKLDPETREFFALSYDVIKKPYLKYFRFSPCGEKSPDVEIPLPQPTMMHDFA 364

Query: 351 ITEKYVVIPDQQVVFKLPEMIRGGSPVVYDKEKTSRFGILDKNATDANAIKWIEAPDCFC 410
           IT+ +V+IPDQQVVFKL EMI GGSPVVYDKEK +RFG+L K A DA+ ++WI+ PDCFC
Sbjct: 365 ITKNFVIIPDQQVVFKLQEMICGGSPVVYDKEKIARFGVLPKYAIDASEMQWIDVPDCFC 424

Query: 411 FHLWNAWEEPETNEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIITE 470
           FHLWN+WEEPET EVVVIGSCMTPPDSIFNE EENL+SVL+EIRLNL TGKSTRRPI+  
Sbjct: 425 FHLWNSWEEPETEEVVVIGSCMTPPDSIFNESEENLQSVLTEIRLNLRTGKSTRRPILRP 484

Query: 471 -TEQVNLEAGMVNRNLLGRKTQFSYLALAEPWPKVSGFAKVDVLSGEVKKYLYGEQSYGG 530
              Q+NLEAGMVNRN LGR+T+F+YLA+AEPWPKVSGFAKVD+ SGE++++ YG+  YGG
Sbjct: 485 GNSQINLEAGMVNRNRLGRRTRFAYLAIAEPWPKVSGFAKVDLASGEIQRFEYGDGGYGG 544

Query: 531 EPLFLPREGAEAEDDGHILAFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTF 590
           EP F+PREG + ED G++LAFVHDE+E  SEL I+NA  + LEA V+LPSRVPYGF+GTF
Sbjct: 545 EPYFVPREGCDREDGGYVLAFVHDEREGSSELLIMNAADMRLEAAVRLPSRVPYGFYGTF 604

Query: 591 ISCKDLQKQ 592
           +S  +L  Q
Sbjct: 605 VSATELHSQ 609

BLAST of CSPI04G07560 vs. TrEMBL
Match: K4KBB8_MOMCH (9-cis-epoxycarotenoid dioxygenase OS=Momordica charantia GN=NCED PE=2 SV=1)

HSP 1 Score: 1023.5 bits (2645), Expect = 1.1e-295
Identity = 504/594 (84.85%), Postives = 543/594 (91.41%), Query Frame = 1

Query: 1   MASSSSS-FLQSPFSSSMPD--KKLSNYTTNSLLSVSSSLHTPSIIQIPKHSHTTFPSPL 60
           MAS+ SS +L+S FS SMP+     SNY  N    VS SLHTPSI+QIPKHS T  P PL
Sbjct: 1   MASAPSSPWLKSGFSPSMPELLSVNSNYVKNR---VSCSLHTPSIVQIPKHSQTFRPPPL 60

Query: 61  QTSIPKPPPLTTTPPVSGSQDWNFLQRAAAMALDAVENALISAERKHSLPKTADPAVQIA 120
           +T + KP     +P  S  +DWNFLQRAA+MA+DAVENAL+SAERK  LPKTADP VQIA
Sbjct: 61  KTPVEKP----VSPSTSAPEDWNFLQRAASMAIDAVENALLSAERKRPLPKTADPEVQIA 120

Query: 121 GNFAPVPEQPVRKGLPVIGKVPDHIRGVYVRNGANPLHEPLSGHHLFDGDGMVHAVEFSE 180
           GNFAPVPEQPVRK LPV G++P+ IRGVYVRNGANPLHEP +GHHLFDGDGMVHAVEFS+
Sbjct: 121 GNFAPVPEQPVRKALPVTGRIPECIRGVYVRNGANPLHEPAAGHHLFDGDGMVHAVEFSD 180

Query: 181 AGGVSYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHNHG 240
            GGVSYACRFTETQRLVQERA+GRPVFPKAIGELHGHSGIARL+LFYARGLFGLVDH+HG
Sbjct: 181 GGGVSYACRFTETQRLVQERAHGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHG 240

Query: 241 IGVANAGLVYFNGRLLAMSEDDLPYQIRVTPAGDLKTVGRFNFDGQLESTMIAHPKLDPV 300
           IGVANAGLVYFNGRLLAMSEDDLPYQIRVTPAGDLKT+GRF+FDGQL+STMIAHPKLDPV
Sbjct: 241 IGVANAGLVYFNGRLLAMSEDDLPYQIRVTPAGDLKTIGRFDFDGQLKSTMIAHPKLDPV 300

Query: 301 SGEMFALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKYVVIPDQQVV 360
           SGE+FA+SYDVIQKP+LKYFKFSPEGEKSPDVE+PLPQPTMMHDFAITEK+VVIPDQQVV
Sbjct: 301 SGELFAISYDVIQKPHLKYFKFSPEGEKSPDVEVPLPQPTMMHDFAITEKFVVIPDQQVV 360

Query: 361 FKLPEMIRGGSPVVYDKEKTSRFGILDKNATDANAIKWIEAPDCFCFHLWNAWEEPETNE 420
           FKLPEMIRGGSPV+YDK K SRFGIL KNATDA  IKWIEAPDCFCFHLWNAWEEPETNE
Sbjct: 361 FKLPEMIRGGSPVIYDKNKISRFGILPKNATDAGDIKWIEAPDCFCFHLWNAWEEPETNE 420

Query: 421 VVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIITETEQVNLEAGMVNRN 480
           VVVIGSCMTPPDSIFNECEENLKSVLSEIRLNL+TGKSTRRPII ETEQVNLEAGMVNRN
Sbjct: 421 VVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLTTGKSTRRPIIPETEQVNLEAGMVNRN 480

Query: 481 LLGRKTQFSYLALAEPWPKVSGFAKVDVLSGEVKKYLYGEQSYGGEPLFLPREGAEAEDD 540
            LGRKTQF+YLALAEPWPKVSGFAKVD+ +GE++KY+YGEQ YGGEPLFLPREGAEAEDD
Sbjct: 481 RLGRKTQFAYLALAEPWPKVSGFAKVDLSTGEIRKYIYGEQRYGGEPLFLPREGAEAEDD 540

Query: 541 GHILAFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISCKDLQKQ 592
           GHILAFVHDEKEWKSELQIVNA+TL+LEA+VKLPSRVPYGFHGTFI  K LQKQ
Sbjct: 541 GHILAFVHDEKEWKSELQIVNAITLKLEASVKLPSRVPYGFHGTFIGSKGLQKQ 587

BLAST of CSPI04G07560 vs. TrEMBL
Match: A0A061F8R1_THECC (Nine-cis-epoxycarotenoid dioxygenase 3 OS=Theobroma cacao GN=TCM_032343 PE=4 SV=1)

HSP 1 Score: 913.3 bits (2359), Expect = 1.5e-262
Identity = 452/593 (76.22%), Postives = 507/593 (85.50%), Query Frame = 1

Query: 2   ASSSSSFLQSPFSSSMPDKKLSNYTTNSLLSVSSSLHTPSIIQIPKHSHTTFPSPLQTSI 61
           ++SSS+      SSS+P K+ +        ++S SL TPSI+  PK S T  PSP   S 
Sbjct: 26  STSSSAPDLGSISSSIPFKRPTIKP-----NISCSLQTPSILHFPKQSPTYPPSPTPASS 85

Query: 62  P--KPPPLTTTPPVSGSQDWNFLQRAAAMALDAVENALISAERKHSLPKTADPAVQIAGN 121
           P  K P  T++     SQ WN  QRAAAMALD VENAL+S ER H LPKTADP VQI+GN
Sbjct: 86  PTTKHPKNTSSAEKPQSQQWNPFQRAAAMALDVVENALVSHERNHPLPKTADPRVQISGN 145

Query: 122 FAPVPEQPVRKGLPVIGKVPDHIRGVYVRNGANPLHEPLSGHHLFDGDGMVHAVEFSEAG 181
           FAPVPEQP++  LPV G +P+ I+GVYVRNGANPLHEP++GHH FDGDGMVHAV+F   G
Sbjct: 146 FAPVPEQPIKHRLPVTGTIPECIQGVYVRNGANPLHEPVAGHHFFDGDGMVHAVQFKN-G 205

Query: 182 GVSYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHNHGIG 241
             SY CRFTET RLVQERA+GRPVFPKAIGELHGHSGIARL+LFYARGLFGLVD +HG G
Sbjct: 206 SASYGCRFTETSRLVQERAFGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDPSHGTG 265

Query: 242 VANAGLVYFNGRLLAMSEDDLPYQIRVTPAGDLKTVGRFNFDGQLESTMIAHPKLDPVSG 301
           VANAGLVYFNG LLAMSEDDLPY +R+TP+GDLKTVGR++FDGQL+STMIAHPK+DPV+G
Sbjct: 266 VANAGLVYFNGHLLAMSEDDLPYHVRITPSGDLKTVGRYDFDGQLKSTMIAHPKVDPVTG 325

Query: 302 EMFALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKYVVIPDQQVVFK 361
           E FALSYDVIQKPYLKYF FS +G+KSPDVEIP+  PTMMHDFAITE +VVIPDQQVVFK
Sbjct: 326 EFFALSYDVIQKPYLKYFHFSADGKKSPDVEIPVESPTMMHDFAITENFVVIPDQQVVFK 385

Query: 362 LPEMIRGGSPVVYDKEKTSRFGILDKNATDANAIKWIEAPDCFCFHLWNAWEEPETNEVV 421
           LPEMI GGSPVVYDK K SRFGILDKNATD + I W+EAPDCFCFHLWNAWEEP+T+EVV
Sbjct: 386 LPEMIHGGSPVVYDKNKMSRFGILDKNATDDSGISWVEAPDCFCFHLWNAWEEPQTDEVV 445

Query: 422 VIGSCMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIITETEQVNLEAGMVNRNLL 481
           VIGSCMTPPDSIFNEC+E+LKSVLSEIRLNL TGKSTRRPII+E+EQVNLEAGMVNRNLL
Sbjct: 446 VIGSCMTPPDSIFNECDESLKSVLSEIRLNLKTGKSTRRPIISESEQVNLEAGMVNRNLL 505

Query: 482 GRKTQFSYLALAEPWPKVSGFAKVDVLSGEVKKYLYGEQSYGGEPLFLPRE-GAEAEDDG 541
           GRKT+F+YLALAEPWPKVSGFAKVD+ +GEVKKY+YG+Q YGGEPLF PR   +E EDDG
Sbjct: 506 GRKTRFAYLALAEPWPKVSGFAKVDLSTGEVKKYIYGDQRYGGEPLFFPRNPNSENEDDG 565

Query: 542 HILAFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISCKDLQKQ 592
           +ILAFVHDEK W+SELQIVNAM L+LEATVKLPSRVPYGFHGTFIS KDL+KQ
Sbjct: 566 YILAFVHDEKTWQSELQIVNAMNLQLEATVKLPSRVPYGFHGTFISSKDLEKQ 612

BLAST of CSPI04G07560 vs. TrEMBL
Match: A5BR72_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_018449 PE=4 SV=1)

HSP 1 Score: 910.2 bits (2351), Expect = 1.3e-261
Identity = 445/589 (75.55%), Postives = 513/589 (87.10%), Query Frame = 1

Query: 5   SSSFLQSPFSSSMPDKKLSNYTTNSLLSVSSSLHTPSIIQIPKH-SHTTFPSPLQTSIPK 64
           SSS   S  SS++     S +      +++ SLH+PS++  PKH S +T  + ++T++PK
Sbjct: 25  SSSSSSSSSSSALDLGSFSFHRPTIRPNINCSLHSPSVLHFPKHTSSSTGVATVKTTLPK 84

Query: 65  PPPLTTTPPVSGSQDWNFLQRAAAMALDAVENALISAERKHSLPKTADPAVQIAGNFAPV 124
                ++   S SQ WNFLQ AAAMALDAVE AL++ E +H LPKTADP VQIAGNFAPV
Sbjct: 85  EKDDFSS---SVSQQWNFLQSAAAMALDAVETALVARETQHPLPKTADPRVQIAGNFAPV 144

Query: 125 PEQPVRKGLPVIGKVPDHIRGVYVRNGANPLHEPLSGHHLFDGDGMVHAVEFSEAGGVSY 184
           PEQPV+  LPV G++PD I+GVY RNGANPLH+P++GHHLFDGDGMVHAV+F + G  SY
Sbjct: 145 PEQPVQHSLPVDGRIPDCIQGVYTRNGANPLHKPVAGHHLFDGDGMVHAVQFKD-GDASY 204

Query: 185 ACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHNHGIGVANA 244
           ACRFTETQRL+QER +GRPVFPKAIGELHGHSGIARL+LFYARGLFGLVDH+HG GVANA
Sbjct: 205 ACRFTETQRLIQERDFGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHGTGVANA 264

Query: 245 GLVYFNGRLLAMSEDDLPYQIRVTPAGDLKTVGRFNFDGQLESTMIAHPKLDPVSGEMFA 304
           GLVYFN RLLAMSEDDLPYQIRVTP+GDL+TVGR++F+GQL STMIAHPK+DPVSGEMFA
Sbjct: 265 GLVYFNRRLLAMSEDDLPYQIRVTPSGDLETVGRYDFEGQLRSTMIAHPKVDPVSGEMFA 324

Query: 305 LSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKYVVIPDQQVVFKLPEM 364
           LSYDV+QKPYLKYF+FSP+G+KSPDVEIPL +PTMMHDFAITE++VV+P+QQVVFKL EM
Sbjct: 325 LSYDVVQKPYLKYFRFSPKGDKSPDVEIPLAEPTMMHDFAITERFVVVPNQQVVFKLQEM 384

Query: 365 IRGGSPVVYDKEKTSRFGILDKNATDANAIKWIEAPDCFCFHLWNAWEEPETNEVVVIGS 424
           I GGSPVVYDK K SRFG+LDKNATDA+ + WIEAPDCFCFHLWNAWEEPET+EVVVIGS
Sbjct: 385 ISGGSPVVYDKNKMSRFGVLDKNATDASGMXWIEAPDCFCFHLWNAWEEPETDEVVVIGS 444

Query: 425 CMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIITETEQVNLEAGMVNRNLLGRKT 484
           CMTPPDSIFNEC+E LKSVLSEIRLNL TGKSTRRPI+ E+EQVNLEAGMVNRN LGRKT
Sbjct: 445 CMTPPDSIFNECDEGLKSVLSEIRLNLKTGKSTRRPILPESEQVNLEAGMVNRNRLGRKT 504

Query: 485 QFSYLALAEPWPKVSGFAKVDVLSGEVKKYLYGEQSYGGEPLFLPRE-GAEAEDDGHILA 544
           QF+YLA+AEPWPKVSGFAKVD+ +G+V KY+YGEQ YGGEPLFLPR+  +  EDDG+ILA
Sbjct: 505 QFAYLAVAEPWPKVSGFAKVDLSTGQVSKYIYGEQRYGGEPLFLPRDPNSGREDDGYILA 564

Query: 545 FVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISCKDLQKQ 592
           FVHDEK WKSELQIVNA  L+LEA+VKLPSRVPYGFHGTFIS K+L+KQ
Sbjct: 565 FVHDEKTWKSELQIVNATNLQLEASVKLPSRVPYGFHGTFISSKELEKQ 609

BLAST of CSPI04G07560 vs. TrEMBL
Match: I7FMA3_VITVI (9-cis epoxy carotenoid dioxygenase 3 OS=Vitis vinifera GN=NCED3 PE=2 SV=1)

HSP 1 Score: 910.2 bits (2351), Expect = 1.3e-261
Identity = 445/589 (75.55%), Postives = 513/589 (87.10%), Query Frame = 1

Query: 5   SSSFLQSPFSSSMPDKKLSNYTTNSLLSVSSSLHTPSIIQIPKH-SHTTFPSPLQTSIPK 64
           SSS   S  SS++     S +      +++ SLH+PS++  PKH S +T  + ++T++PK
Sbjct: 25  SSSSSSSSSSSALDLGSFSFHRPTIRPNINCSLHSPSVLHFPKHTSSSTGVATVKTTLPK 84

Query: 65  PPPLTTTPPVSGSQDWNFLQRAAAMALDAVENALISAERKHSLPKTADPAVQIAGNFAPV 124
                ++   S SQ WNFLQ AAAMALDAVE AL++ E +H LPKTADP VQIAGNFAPV
Sbjct: 85  EKDDFSS---SVSQQWNFLQSAAAMALDAVETALVARETQHPLPKTADPRVQIAGNFAPV 144

Query: 125 PEQPVRKGLPVIGKVPDHIRGVYVRNGANPLHEPLSGHHLFDGDGMVHAVEFSEAGGVSY 184
           PEQPV+  LPV G++PD I+GVY RNGANPLH+P++GHHLFDGDGMVHAV+F + G  SY
Sbjct: 145 PEQPVQHSLPVDGRIPDCIQGVYTRNGANPLHKPVAGHHLFDGDGMVHAVQFKD-GDASY 204

Query: 185 ACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHNHGIGVANA 244
           ACRFTETQRL+QER +GRPVFPKAIGELHGHSGIARL+LFYARGLFGLVDH+HG GVANA
Sbjct: 205 ACRFTETQRLIQERDFGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHGTGVANA 264

Query: 245 GLVYFNGRLLAMSEDDLPYQIRVTPAGDLKTVGRFNFDGQLESTMIAHPKLDPVSGEMFA 304
           GLVYFN RLLAMSEDDLPYQIRVTP+GDL+TVGR++F+GQL STMIAHPK+DPVSGEMFA
Sbjct: 265 GLVYFNRRLLAMSEDDLPYQIRVTPSGDLETVGRYDFEGQLRSTMIAHPKVDPVSGEMFA 324

Query: 305 LSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKYVVIPDQQVVFKLPEM 364
           LSYDV+QKPYLKYF+FSP+G+KSPDVEIPL +PTMMHDFAITE++VV+PDQQVVFKL EM
Sbjct: 325 LSYDVVQKPYLKYFRFSPKGDKSPDVEIPLAEPTMMHDFAITERFVVVPDQQVVFKLQEM 384

Query: 365 IRGGSPVVYDKEKTSRFGILDKNATDANAIKWIEAPDCFCFHLWNAWEEPETNEVVVIGS 424
           I  GSPVVYDK K SRFG+LDKNATDA+ ++WIEAPDCFCFHLWNAWEEPET+EVVVIGS
Sbjct: 385 ISRGSPVVYDKNKMSRFGVLDKNATDASGMRWIEAPDCFCFHLWNAWEEPETDEVVVIGS 444

Query: 425 CMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIITETEQVNLEAGMVNRNLLGRKT 484
           CMTPPDSIFNEC+E LKSVLSEIRLNL TGKSTRRPI+ E+EQVNLEAGMVNRN LGRKT
Sbjct: 445 CMTPPDSIFNECDEGLKSVLSEIRLNLKTGKSTRRPILPESEQVNLEAGMVNRNRLGRKT 504

Query: 485 QFSYLALAEPWPKVSGFAKVDVLSGEVKKYLYGEQSYGGEPLFLPRE-GAEAEDDGHILA 544
           QF+YLA+AEPWPKVSGFAKVD+ +G+V KY+YGEQ YGGEPLFLPR+  +  EDDG+ILA
Sbjct: 505 QFAYLAVAEPWPKVSGFAKVDLSTGQVSKYIYGEQRYGGEPLFLPRDPNSGREDDGYILA 564

Query: 545 FVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISCKDLQKQ 592
           FVHDEK WKSELQIVNA  L+LEA+VKLPSRVPYGFHGTFIS K+L+KQ
Sbjct: 565 FVHDEKTWKSELQIVNATNLQLEASVKLPSRVPYGFHGTFISSKELEKQ 609

BLAST of CSPI04G07560 vs. TrEMBL
Match: Q5SGD1_VITVI (9-cis-epoxycarotenoid dioxygenase 1 OS=Vitis vinifera GN=NCED1 PE=2 SV=1)

HSP 1 Score: 909.8 bits (2350), Expect = 1.7e-261
Identity = 445/589 (75.55%), Postives = 513/589 (87.10%), Query Frame = 1

Query: 5   SSSFLQSPFSSSMPDKKLSNYTTNSLLSVSSSLHTPSIIQIPKH-SHTTFPSPLQTSIPK 64
           SSS   S  SS++     S +      +++ SLH+PS++  PKH S +T  + ++T++PK
Sbjct: 25  SSSSSSSSSSSALDLGSFSFHRPTIRPNINCSLHSPSVLHFPKHTSSSTGVATVKTTLPK 84

Query: 65  PPPLTTTPPVSGSQDWNFLQRAAAMALDAVENALISAERKHSLPKTADPAVQIAGNFAPV 124
                ++   S SQ WNFLQ AAAMALDAVE AL++ E +H LPKTADP VQIAGNFAPV
Sbjct: 85  EKDDFSS---SVSQQWNFLQSAAAMALDAVETALVARETQHPLPKTADPRVQIAGNFAPV 144

Query: 125 PEQPVRKGLPVIGKVPDHIRGVYVRNGANPLHEPLSGHHLFDGDGMVHAVEFSEAGGVSY 184
           PEQPV+  LPV G++PD I+GVY RNGANPLH+P++GHHLFDGDGMVHAV+F + G  SY
Sbjct: 145 PEQPVQHSLPVDGRIPDCIQGVYTRNGANPLHKPVAGHHLFDGDGMVHAVQFKD-GDASY 204

Query: 185 ACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHNHGIGVANA 244
           ACRFTETQRL+QER +GRPVFPKAIGELHGHSGIARL+LFYARGLFGLVDH+HG GVANA
Sbjct: 205 ACRFTETQRLIQERDFGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHGTGVANA 264

Query: 245 GLVYFNGRLLAMSEDDLPYQIRVTPAGDLKTVGRFNFDGQLESTMIAHPKLDPVSGEMFA 304
           GLVYFN RLLAMSEDDLPYQIRVTP+GDL+TVGR++F+GQL STMIAHPK+DPVSGEMFA
Sbjct: 265 GLVYFNRRLLAMSEDDLPYQIRVTPSGDLETVGRYDFEGQLRSTMIAHPKVDPVSGEMFA 324

Query: 305 LSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKYVVIPDQQVVFKLPEM 364
           LSYDV+QKPYLKYF+FSP+G+KSPDVEIPL +PTMMHDFAITE++VV+P+QQVVFKL EM
Sbjct: 325 LSYDVVQKPYLKYFRFSPKGDKSPDVEIPLAEPTMMHDFAITERFVVVPNQQVVFKLQEM 384

Query: 365 IRGGSPVVYDKEKTSRFGILDKNATDANAIKWIEAPDCFCFHLWNAWEEPETNEVVVIGS 424
           I GGSPVVYDK K SRFG+LDKNATDA+ + WIEAPDCFCFHLWNAWEEPET+EVVVIGS
Sbjct: 385 ISGGSPVVYDKNKMSRFGVLDKNATDASGMLWIEAPDCFCFHLWNAWEEPETDEVVVIGS 444

Query: 425 CMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIITETEQVNLEAGMVNRNLLGRKT 484
           CMTPPDSIFNEC+E LKSVLSEIRLNL TGKSTRRPI+ E+EQVNLEAGMVNRN LGRKT
Sbjct: 445 CMTPPDSIFNECDEGLKSVLSEIRLNLKTGKSTRRPILPESEQVNLEAGMVNRNRLGRKT 504

Query: 485 QFSYLALAEPWPKVSGFAKVDVLSGEVKKYLYGEQSYGGEPLFLPRE-GAEAEDDGHILA 544
           QF+YLA+AEPWPKVSGFAKVD+ +G+V KY+YGEQ YGGEPLFLPR+  +  EDDG+ILA
Sbjct: 505 QFAYLAVAEPWPKVSGFAKVDLSTGQVSKYIYGEQRYGGEPLFLPRDPNSGREDDGYILA 564

Query: 545 FVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISCKDLQKQ 592
           FVHDEK WKSELQIVNA  L+LEA+VKLPSRVPYGFHGTFIS K+L+KQ
Sbjct: 565 FVHDEKTWKSELQIVNATNLQLEASVKLPSRVPYGFHGTFISSKELEKQ 609

BLAST of CSPI04G07560 vs. TAIR10
Match: AT3G14440.1 (AT3G14440.1 nine-cis-epoxycarotenoid dioxygenase 3)

HSP 1 Score: 871.7 bits (2251), Expect = 2.6e-253
Identity = 438/593 (73.86%), Postives = 496/593 (83.64%), Query Frame = 1

Query: 1   MASSSSSFLQSPFSSSMPDKKLSNYTTNSLLSVSSSLHTPSIIQIPKHSHTTFPSPLQTS 60
           ++SS SS L   + SS+P   +++  T  L +VSS+LHTP  +  PK S     SP    
Sbjct: 24  LSSSQSSDLS--YCSSLP---MASRVTRKL-NVSSALHTPPALHFPKQSSN---SPAIVV 83

Query: 61  IPKPPPLTTTPPVSGSQDWNFLQRAAAMALDAVENALISAERKHSLPKTADPAVQIAGNF 120
            PK          S ++  N  QRAAA ALDA E  L+S E+ H LPKTADP+VQIAGNF
Sbjct: 84  KPKAKE-------SNTKQMNLFQRAAAAALDAAEGFLVSHEKLHPLPKTADPSVQIAGNF 143

Query: 121 APVPEQPVRKGLPVIGKVPDHIRGVYVRNGANPLHEPLSGHHLFDGDGMVHAVEFSEAGG 180
           APV EQPVR+ LPV+GK+PD I+GVYVRNGANPLHEP++GHH FDGDGMVHAV+F E G 
Sbjct: 144 APVNEQPVRRNLPVVGKLPDSIKGVYVRNGANPLHEPVTGHHFFDGDGMVHAVKF-EHGS 203

Query: 181 VSYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHNHGIGV 240
            SYACRFT+T R VQER  GRPVFPKAIGELHGH+GIARLMLFYAR   G+VD  HG GV
Sbjct: 204 ASYACRFTQTNRFVQERQLGRPVFPKAIGELHGHTGIARLMLFYARAAAGIVDPAHGTGV 263

Query: 241 ANAGLVYFNGRLLAMSEDDLPYQIRVTPAGDLKTVGRFNFDGQLESTMIAHPKLDPVSGE 300
           ANAGLVYFNGRLLAMSEDDLPYQ+++TP GDLKTVGRF+FDGQLESTMIAHPK+DP SGE
Sbjct: 264 ANAGLVYFNGRLLAMSEDDLPYQVQITPNGDLKTVGRFDFDGQLESTMIAHPKVDPESGE 323

Query: 301 MFALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKYVVIPDQQVVFKL 360
           +FALSYDV+ KPYLKYF+FSP+G KSPDVEI L QPTMMHDFAITE +VV+PDQQVVFKL
Sbjct: 324 LFALSYDVVSKPYLKYFRFSPDGTKSPDVEIQLDQPTMMHDFAITENFVVVPDQQVVFKL 383

Query: 361 PEMIRGGSPVVYDKEKTSRFGILDKNATDANAIKWIEAPDCFCFHLWNAWEEPETNEVVV 420
           PEMIRGGSPVVYDK K +RFGILDK A D++ IKWI+APDCFCFHLWNAWEEPET+EVVV
Sbjct: 384 PEMIRGGSPVVYDKNKVARFGILDKYAEDSSNIKWIDAPDCFCFHLWNAWEEPETDEVVV 443

Query: 421 IGSCMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIIT-ETEQVNLEAGMVNRNLL 480
           IGSCMTPPDSIFNE +ENLKSVLSEIRLNL TG+STRRPII+ E +QVNLEAGMVNRN+L
Sbjct: 444 IGSCMTPPDSIFNESDENLKSVLSEIRLNLKTGESTRRPIISNEDQQVNLEAGMVNRNML 503

Query: 481 GRKTQFSYLALAEPWPKVSGFAKVDVLSGEVKKYLYGEQSYGGEPLFLPREGAEAEDDGH 540
           GRKT+F+YLALAEPWPKVSGFAKVD+ +GEVKK+LYG+  YGGEPLFLP EG E ED+G+
Sbjct: 504 GRKTKFAYLALAEPWPKVSGFAKVDLTTGEVKKHLYGDNRYGGEPLFLPGEGGE-EDEGY 563

Query: 541 ILAFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISCKDLQKQI 593
           IL FVHDEK WKSELQIVNA++LE+EATVKLPSRVPYGFHGTFI   DL KQ+
Sbjct: 564 ILCFVHDEKTWKSELQIVNAVSLEVEATVKLPSRVPYGFHGTFIGADDLAKQV 598

BLAST of CSPI04G07560 vs. TAIR10
Match: AT1G78390.1 (AT1G78390.1 nine-cis-epoxycarotenoid dioxygenase 9)

HSP 1 Score: 813.9 bits (2101), Expect = 6.4e-236
Identity = 415/603 (68.82%), Postives = 489/603 (81.09%), Query Frame = 1

Query: 1   MASSSSSFLQSPFSSSMPDK--KLSNYTTNSLLSVSSSLHTPSIIQIPKHSHTTFPSPLQ 60
           + S+S+ FL   FS+S      KL + + +S L  +  L  P  +    +  ++  S LQ
Sbjct: 57  LPSTSTQFLDRTFSTSSSSSRPKLQSLSFSSTLR-NKKLVVPCYVSSSVNKKSSVSSSLQ 116

Query: 61  TSIPKPPP-------LTTTPPVSGSQD--WNFLQRAAAMALDAVENALISAERK-HSLPK 120
           +   KPP        +T   P + +Q+   N +QR AAM LDAVENA+IS ER+ H  PK
Sbjct: 117 SPTFKPPSWKKLCNDVTNLIPKTTNQNPKLNPVQRTAAMVLDAVENAMISHERRRHPHPK 176

Query: 121 TADPAVQIAGNFAPVPEQPVRKGLPVIGKVPDHIRGVYVRNGANPLHEPLSGHHLFDGDG 180
           TADPAVQIAGNF PVPE+PV   LPV G VP+ I+GVYVRNGANPLH+P+SGHHLFDGDG
Sbjct: 177 TADPAVQIAGNFFPVPEKPVVHNLPVTGTVPECIQGVYVRNGANPLHKPVSGHHLFDGDG 236

Query: 181 MVHAVEFSEAGGVSYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGL 240
           MVHAV F   G VSYACRFTET RLVQER  GRPVFPKAIGELHGH GIA+LMLF  RGL
Sbjct: 237 MVHAVRFDN-GSVSYACRFTETNRLVQERECGRPVFPKAIGELHGHLGIAKLMLFNTRGL 296

Query: 241 FGLVDHNHGIGVANAGLVYFNGRLLAMSEDDLPYQIRVTPAGDLKTVGRFNFDGQLESTM 300
           FGLVD   G+GVANAGLVYFNG LLAMSEDDLPY ++VT  GDL+T GR++FDGQL+STM
Sbjct: 297 FGLVDPTGGLGVANAGLVYFNGHLLAMSEDDLPYHVKVTQTGDLETSGRYDFDGQLKSTM 356

Query: 301 IAHPKLDPVSGEMFALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKY 360
           IAHPK+DP + E+FALSYDV+ KPYLKYF+F+ +GEKSPDVEIPL QPTM+HDFAITE +
Sbjct: 357 IAHPKIDPETRELFALSYDVVSKPYLKYFRFTSDGEKSPDVEIPLDQPTMIHDFAITENF 416

Query: 361 VVIPDQQVVFKLPEMIRGGSPVVYDKEKTSRFGILDKNATDANAIKWIEAPDCFCFHLWN 420
           VVIPDQQVVF+LPEMIRGGSPVVYD++K SRFGIL+KNA DA++I+WIE PDCFCFHLWN
Sbjct: 417 VVIPDQQVVFRLPEMIRGGSPVVYDEKKKSRFGILNKNAKDASSIQWIEVPDCFCFHLWN 476

Query: 421 AWEEPETNEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIITETEQVN 480
           +WEEPET+EVVVIGSCMTPPDSIFNE +E L+SVLSEIRLNL TG+STRRP+I  +EQVN
Sbjct: 477 SWEEPETDEVVVIGSCMTPPDSIFNEHDETLQSVLSEIRLNLKTGESTRRPVI--SEQVN 536

Query: 481 LEAGMVNRNLLGRKTQFSYLALAEPWPKVSGFAKVDVLSGEVKKYLYGEQSYGGEPLFLP 540
           LEAGMVNRNLLGRKT+++YLAL EPWPKVSGFAKVD+ +GE++KY+YGE  YGGEPLFLP
Sbjct: 537 LEAGMVNRNLLGRKTRYAYLALTEPWPKVSGFAKVDLSTGEIRKYIYGEGKYGGEPLFLP 596

Query: 541 REGAEAEDDGHILAFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISCKDL 592
             G   ED G+I+ FVHDE++ KSELQ++NA+ ++LEATV LPSRVPYGFHGTFIS +DL
Sbjct: 597 -SGDGEEDGGYIMVFVHDEEKVKSELQLINAVNMKLEATVTLPSRVPYGFHGTFISKEDL 654

BLAST of CSPI04G07560 vs. TAIR10
Match: AT1G30100.1 (AT1G30100.1 nine-cis-epoxycarotenoid dioxygenase 5)

HSP 1 Score: 796.2 bits (2055), Expect = 1.4e-230
Identity = 395/589 (67.06%), Postives = 462/589 (78.44%), Query Frame = 1

Query: 7   SFLQSPFSSSMPDKKLSNYTTNSL---LSVSSSLHTPSIIQIPKHSHTTFPSPLQTSIPK 66
           SF  S   +  P   +S   T      LS +S   TP+++  P +             P 
Sbjct: 17  SFAPSDLDAPSPSSSVSFTNTKPRRRKLSANSVSDTPNLLNFPNY-------------PS 76

Query: 67  PPPLTTTPPVSGSQDWNFLQRAAAMALDAVENALISAERKHSLPKTADPAVQIAGNFAPV 126
           P P+    P   +  WN LQRAA+ ALD  E AL+  ER   LPKT DP  QI+GN+APV
Sbjct: 77  PNPII---PEKDTSRWNPLQRAASAALDFAETALLRRERSKPLPKTVDPRHQISGNYAPV 136

Query: 127 PEQPVRKGLPVIGKVPDHIRGVYVRNGANPLHEPLSGHHLFDGDGMVHAVEFSEAGGVSY 186
           PEQ V+  L V GK+PD I GVY+RNGANPL EP+SGHHLFDGDGMVHAV+ +  G  SY
Sbjct: 137 PEQSVKSSLSVDGKIPDCIDGVYLRNGANPLFEPVSGHHLFDGDGMVHAVKITN-GDASY 196

Query: 187 ACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHNHGIGVANA 246
           +CRFTET+RLVQE+  G P+FPKAIGELHGHSGIARLMLFYARGLFGL++H +G GVANA
Sbjct: 197 SCRFTETERLVQEKQLGSPIFPKAIGELHGHSGIARLMLFYARGLFGLLNHKNGTGVANA 256

Query: 247 GLVYFNGRLLAMSEDDLPYQIRVTPAGDLKTVGRFNFDGQLESTMIAHPKLDPVSGEMFA 306
           GLVYF+ RLLAMSEDDLPYQ+RVT  GDL+T+GRF+FDGQL S MIAHPK+DPV+ E+FA
Sbjct: 257 GLVYFHDRLLAMSEDDLPYQVRVTDNGDLETIGRFDFDGQLSSAMIAHPKIDPVTKELFA 316

Query: 307 LSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKYVVIPDQQVVFKLPEM 366
           LSYDV++KPYLKYFKFSPEGEKSPDVEIPL  PTMMHDFAITE +VVIPDQQVVFKL +M
Sbjct: 317 LSYDVVKKPYLKYFKFSPEGEKSPDVEIPLASPTMMHDFAITENFVVIPDQQVVFKLSDM 376

Query: 367 IRGGSPVVYDKEKTSRFGILDKNATDANAIKWIEAPDCFCFHLWNAWEEPETNEVVVIGS 426
             G SPV YD EK SRFGIL +NA DA+ + W+E+P+ FCFHLWNAWE PET+EVVVIGS
Sbjct: 377 FLGKSPVKYDGEKISRFGILPRNAKDASEMVWVESPETFCFHLWNAWESPETDEVVVIGS 436

Query: 427 CMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIITETEQVNLEAGMVNRNLLGRKT 486
           CMTP DSIFNEC+E L SVLSEIRLNL TGKSTRR II  + Q+NLEAGMVNRNLLGRKT
Sbjct: 437 CMTPADSIFNECDEQLNSVLSEIRLNLKTGKSTRRTIIPGSVQMNLEAGMVNRNLLGRKT 496

Query: 487 QFSYLALAEPWPKVSGFAKVDVLSGEVKKYLYGEQSYGGEPLFLPRE-GAEAEDDGHILA 546
           +++YLA+AEPWPKVSGFAKVD+ +GEVK + YG + YGGEP FLPR   ++ EDDG+I++
Sbjct: 497 RYAYLAIAEPWPKVSGFAKVDLSTGEVKNHFYGGKKYGGEPFFLPRGLESDGEDDGYIMS 556

Query: 547 FVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISCKDLQKQ 592
           FVHDE+ W+SEL IVNA+TLELEATVKLPSRVPYGFHGTF++  D+  Q
Sbjct: 557 FVHDEESWESELHIVNAVTLELEATVKLPSRVPYGFHGTFVNSADMLNQ 588

BLAST of CSPI04G07560 vs. TAIR10
Match: AT4G18350.1 (AT4G18350.1 nine-cis-epoxycarotenoid dioxygenase 2)

HSP 1 Score: 756.1 bits (1951), Expect = 1.6e-218
Identity = 362/531 (68.17%), Postives = 435/531 (81.92%), Query Frame = 1

Query: 65  PPLTTTPPVSGSQDWNFLQRAAAMALDAVENALISAERKHSLPKTADPAVQIAGNFAPVP 124
           P  T TPP       N  Q+AAA+A+DA E ALIS E+   LPKTADP VQIAGN++PVP
Sbjct: 55  PRTTATPPQHNPLRLNIFQKAAAIAIDAAERALISHEQDSPLPKTADPRVQIAGNYSPVP 114

Query: 125 EQPVRKGLPVIGKVPDHIRGVYVRNGANPLHEPLSGHHLFDGDGMVHAVEFSEAGGVSYA 184
           E  VR+ L V G +PD I GVY+RNGANP+ EP +GHHLFDGDGMVHAV+ +  G  SYA
Sbjct: 115 ESSVRRNLTVEGTIPDCIDGVYIRNGANPMFEPTAGHHLFDGDGMVHAVKITN-GSASYA 174

Query: 185 CRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHNHGIGVANAG 244
           CRFT+T+RLVQE+  GRPVFPKAIGELHGHSGIARLMLFYARGL GL+++ +G+GVANAG
Sbjct: 175 CRFTKTERLVQEKRLGRPVFPKAIGELHGHSGIARLMLFYARGLCGLINNQNGVGVANAG 234

Query: 245 LVYFNGRLLAMSEDDLPYQIRVTPAGDLKTVGRFNFDGQLESTMIAHPKLDPVSGEMFAL 304
           LVYFN RLLAMSEDDLPYQ+++T  GDL+TVGR++FDGQL+S MIAHPKLDPV+ E+ AL
Sbjct: 235 LVYFNNRLLAMSEDDLPYQLKITQTGDLQTVGRYDFDGQLKSAMIAHPKLDPVTKELHAL 294

Query: 305 SYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKYVVIPDQQVVFKLPEMI 364
           SYDV++KPYLKYF+FSP+G KSP++EIPL  PTM+HDFAITE +VVIPDQQVVFKL EMI
Sbjct: 295 SYDVVKKPYLKYFRFSPDGVKSPELEIPLETPTMIHDFAITENFVVIPDQQVVFKLGEMI 354

Query: 365 RGGSPVVYDKEKTSRFGILDKNATDANAIKWIEAPDCFCFHLWNAWEEPETNEVVVIGSC 424
            G SPVV+D EK SR GI+ K+AT+A+ I W+ +P+ FCFHLWNAWE PET E+VVIGSC
Sbjct: 355 SGKSPVVFDGEKVSRLGIMPKDATEASQIIWVNSPETFCFHLWNAWESPETEEIVVIGSC 414

Query: 425 MTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIITETEQVNLEAGMVNRNLLGRKTQ 484
           M+P DSIFNE +E+L+SVLSEIR+NL T K+TRR ++   E VNLE GMVNRN LGRKT+
Sbjct: 415 MSPADSIFNERDESLRSVLSEIRINLRTRKTTRRSLLVN-EDVNLEIGMVNRNRLGRKTR 474

Query: 485 FSYLALAEPWPKVSGFAKVDVLSGEVKKYLYGEQSYGGEPLFLP---REGAEAEDDGHIL 544
           F++LA+A PWPKVSGFAKVD+ +GE+KKY+YG + YGGEP FLP     G E EDDG+I 
Sbjct: 475 FAFLAIAYPWPKVSGFAKVDLCTGEMKKYIYGGEKYGGEPFFLPGNSGNGEENEDDGYIF 534

Query: 545 AFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISCKDLQKQI 593
             VHDE+   SELQI+NA+ L+LEAT+KLPSRVPYGFHGTF+   +L  Q+
Sbjct: 535 CHVHDEETKTSELQIINAVNLKLEATIKLPSRVPYGFHGTFVDSNELVDQL 583

BLAST of CSPI04G07560 vs. TAIR10
Match: AT3G24220.1 (AT3G24220.1 nine-cis-epoxycarotenoid dioxygenase 6)

HSP 1 Score: 638.6 bits (1646), Expect = 3.7e-183
Identity = 321/587 (54.68%), Postives = 423/587 (72.06%), Query Frame = 1

Query: 11  SPFSSSMPDKKLSNYTTNSLLSVSSSLHTPSIIQIPKHSHTTFPSPLQTSIPKPPPLTTT 70
           SP S  +P  K +N +   L++           +IP     T P  L + +P P  L  T
Sbjct: 15  SPRSHLLPQPKNANISRRILINP---------FKIP-----TLPD-LTSPVPSPVKLKPT 74

Query: 71  PPVSGSQDWNFLQRAAAMALDAVENAL-ISAERKHSLPKTADPAVQIAGNFAPVPEQPVR 130
            P     + N LQ+ AA  LD +E+++ I  E+   LPK  DPAVQ++GNFAPV E PV+
Sbjct: 75  YP-----NLNLLQKLAATMLDKIESSIVIPMEQNRPLPKPTDPAVQLSGNFAPVNECPVQ 134

Query: 131 KGLPVIGKVPDHIRGVYVRNGANPLHEPLSGHHLFDGDGMVHAVEFSEAGGVSYACRFTE 190
            GL V+G++P  ++GVY+RNGANP+  PL+GHHLFDGDGM+HAV       VSY+CR+T+
Sbjct: 135 NGLEVVGQIPSCLKGVYIRNGANPMFPPLAGHHLFDGDGMIHAVSIGFDNQVSYSCRYTK 194

Query: 191 TQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHNHGIGVANAGLVYFN 250
           T RLVQE A GR VFPK IGELHGHSG+ARL LF AR   GLVD   G+GVANAG+V+FN
Sbjct: 195 TNRLVQETALGRSVFPKPIGELHGHSGLARLALFTARAGIGLVDGTRGMGVANAGVVFFN 254

Query: 251 GRLLAMSEDDLPYQIRVTPAGDLKTVGRFNFDGQLESTMIAHPKLDPVSGEMFALSYDVI 310
           GRLLAMSEDDLPYQ+++   GDL+T+GRF FD Q++S++IAHPK+D  +G++  LSY+V+
Sbjct: 255 GRLLAMSEDDLPYQVKIDGQGDLETIGRFGFDDQIDSSVIAHPKVDATTGDLHTLSYNVL 314

Query: 311 QKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKYVVIPDQQVVFKLPEMIRGGSP 370
           +KP+L+Y KF+  G+K+ DVEI LP+PTM+HDFAITE +VVIPDQQ+VFKL EMIRGGSP
Sbjct: 315 KKPHLRYLKFNTCGKKTRDVEITLPEPTMIHDFAITENFVVIPDQQMVFKLSEMIRGGSP 374

Query: 371 VVYDKEKTSRFGILDKNATDANAIKWIEAPDCFCFHLWNAWEEPETNE----VVVIGSCM 430
           V+Y KEK +RFG+L K     + I W++ PDCFCFHLWNAWEE  T E    +VVIGSCM
Sbjct: 375 VIYVKEKMARFGVLSKQDLTGSDINWVDVPDCFCFHLWNAWEE-RTEEGDPVIVVIGSCM 434

Query: 431 TPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIITETEQVNLEAGMVNRNLLGRKTQF 490
           +PPD+IF+E  E  +  LSEIRLN+ T +S R+ I+T    VNLEAG +NR+ +GRK+QF
Sbjct: 435 SPPDTIFSESGEPTRVELSEIRLNMRTKESNRKVIVT---GVNLEAGHINRSYVGRKSQF 494

Query: 491 SYLALAEPWPKVSGFAKVDVLSGEVKKYLYGEQSYGGEPLFLPREGAEAEDDGHILAFVH 550
            Y+A+A+PWPK SG AKVD+ +G V ++ YG   +GGEP F+P EG   ED G+++ FV 
Sbjct: 495 VYIAIADPWPKCSGIAKVDIQNGTVSEFNYGPSRFGGEPCFVP-EGEGEEDKGYVMGFVR 554

Query: 551 DEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISCKDLQKQI 593
           DE++ +SE  +V+A  ++  A V+LP RVPYGFHGTF+S   L++Q+
Sbjct: 555 DEEKDESEFVVVDATDMKQVAAVRLPERVPYGFHGTFVSENQLKEQV 576

BLAST of CSPI04G07560 vs. NCBI nr
Match: gi|449460068|ref|XP_004147768.1| (PREDICTED: 9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like [Cucumis sativus])

HSP 1 Score: 1196.4 bits (3094), Expect = 0.0e+00
Identity = 589/592 (99.49%), Postives = 590/592 (99.66%), Query Frame = 1

Query: 2   ASSSSSFLQSPFSSSMPDKKLSNYTTNSLLSVSSSLHTPSIIQIPKHSHTTFPSPLQTSI 61
           +SSSSSFLQSPFSSSMPDKKLSNYTTN LLSVSSSLHTPSIIQIPKHSHTTFPSPLQTSI
Sbjct: 3   SSSSSSFLQSPFSSSMPDKKLSNYTTNRLLSVSSSLHTPSIIQIPKHSHTTFPSPLQTSI 62

Query: 62  PKPPPLTTTPPVSGSQDWNFLQRAAAMALDAVENALISAERKHSLPKTADPAVQIAGNFA 121
           PKPPPLTTTPPVSGSQDWNFLQRAAAMALDAVENALISAERKHSLPKTADPAVQIAGNFA
Sbjct: 63  PKPPPLTTTPPVSGSQDWNFLQRAAAMALDAVENALISAERKHSLPKTADPAVQIAGNFA 122

Query: 122 PVPEQPVRKGLPVIGKVPDHIRGVYVRNGANPLHEPLSGHHLFDGDGMVHAVEFSEAGGV 181
           PVPEQPVRKGLPVIGKVPDHIRGVYVRNGANPLHEPLSGHHLFDGDGMVHAVEFSEAGGV
Sbjct: 123 PVPEQPVRKGLPVIGKVPDHIRGVYVRNGANPLHEPLSGHHLFDGDGMVHAVEFSEAGGV 182

Query: 182 SYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHNHGIGVA 241
           SYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHNHGIGVA
Sbjct: 183 SYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHNHGIGVA 242

Query: 242 NAGLVYFNGRLLAMSEDDLPYQIRVTPAGDLKTVGRFNFDGQLESTMIAHPKLDPVSGEM 301
           NAGLVYFNGRLLAMSEDDLPYQIRVTPAGDLKTVGRFNFDGQLESTMIAHPKLDPVSGEM
Sbjct: 243 NAGLVYFNGRLLAMSEDDLPYQIRVTPAGDLKTVGRFNFDGQLESTMIAHPKLDPVSGEM 302

Query: 302 FALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKYVVIPDQQVVFKLP 361
           FALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKYVVIPDQQVVFKLP
Sbjct: 303 FALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKYVVIPDQQVVFKLP 362

Query: 362 EMIRGGSPVVYDKEKTSRFGILDKNATDANAIKWIEAPDCFCFHLWNAWEEPETNEVVVI 421
           EMIRGGSPVVYDKEKTSRFGILDKNATDANAIKWIEAPDCFCFHLWNAWEEPETNEVVVI
Sbjct: 363 EMIRGGSPVVYDKEKTSRFGILDKNATDANAIKWIEAPDCFCFHLWNAWEEPETNEVVVI 422

Query: 422 GSCMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIITETEQVNLEAGMVNRNLLGR 481
           GSCMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIITETEQVNLEAGMVNRNLLGR
Sbjct: 423 GSCMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIITETEQVNLEAGMVNRNLLGR 482

Query: 482 KTQFSYLALAEPWPKVSGFAKVDVLSGEVKKYLYGEQSYGGEPLFLPREGAEAEDDGHIL 541
           KTQFSYLALAEPWPKVSGFAKVDVLSGEVKKYLYGEQ YGGEPLFLPREGAEAEDDGHIL
Sbjct: 483 KTQFSYLALAEPWPKVSGFAKVDVLSGEVKKYLYGEQRYGGEPLFLPREGAEAEDDGHIL 542

Query: 542 AFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISCKDLQKQIR 594
           AFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISCKDLQKQIR
Sbjct: 543 AFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISCKDLQKQIR 594

BLAST of CSPI04G07560 vs. NCBI nr
Match: gi|659131081|ref|XP_008465499.1| (PREDICTED: 9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like [Cucumis melo])

HSP 1 Score: 1153.7 bits (2983), Expect = 0.0e+00
Identity = 564/590 (95.59%), Postives = 581/590 (98.47%), Query Frame = 1

Query: 4   SSSSFLQSPFSSSMPDKKLSNYTTNSLLSVSSSLHTPSIIQIPKHSHTTFPSPLQTSIPK 63
           +SSSFL+SPFSSSMPDK LSNYT+  LLSVSSSLHTPSIIQIPKHSHTTFPSPL+TSIPK
Sbjct: 2   ASSSFLKSPFSSSMPDKTLSNYTSRRLLSVSSSLHTPSIIQIPKHSHTTFPSPLKTSIPK 61

Query: 64  PPPLTTTPPVSGSQDWNFLQRAAAMALDAVENALISAERKHSLPKTADPAVQIAGNFAPV 123
           PPPL+TTPP+SGS+DWNFLQRAAAMALDAVENALISAERKHSLPKTADPAVQIAGNFAPV
Sbjct: 62  PPPLSTTPPLSGSEDWNFLQRAAAMALDAVENALISAERKHSLPKTADPAVQIAGNFAPV 121

Query: 124 PEQPVRKGLPVIGKVPDHIRGVYVRNGANPLHEPLSGHHLFDGDGMVHAVEFSEAGGVSY 183
           PEQPVRKGLPVIGKVP+ IRGVYVRNGANPLHEP+SGHHLFDGDGMVHAVEFSEAGGVSY
Sbjct: 122 PEQPVRKGLPVIGKVPECIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFSEAGGVSY 181

Query: 184 ACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHNHGIGVANA 243
           ACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHN GIGVANA
Sbjct: 182 ACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHNQGIGVANA 241

Query: 244 GLVYFNGRLLAMSEDDLPYQIRVTPAGDLKTVGRFNFDGQLESTMIAHPKLDPVSGEMFA 303
           GLVYFNGRLLAMSEDDLPYQIRVTPAGDLKTVGRF+FDGQL+STMIAHPKLDPVSGEMFA
Sbjct: 242 GLVYFNGRLLAMSEDDLPYQIRVTPAGDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEMFA 301

Query: 304 LSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKYVVIPDQQVVFKLPEM 363
           LSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKYVVIPDQQVVFKLPEM
Sbjct: 302 LSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKYVVIPDQQVVFKLPEM 361

Query: 364 IRGGSPVVYDKEKTSRFGILDKNATDANAIKWIEAPDCFCFHLWNAWEEPETNEVVVIGS 423
           IRGGSPVVYDKEKTSRFGILDKNA DANAIKWIEAPDCFCFHLWNAWEEPETNE+VVIGS
Sbjct: 362 IRGGSPVVYDKEKTSRFGILDKNAADANAIKWIEAPDCFCFHLWNAWEEPETNEIVVIGS 421

Query: 424 CMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIITETEQVNLEAGMVNRNLLGRKT 483
           CMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPII+ETEQVNLEAGMVNRNLLGRKT
Sbjct: 422 CMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIISETEQVNLEAGMVNRNLLGRKT 481

Query: 484 QFSYLALAEPWPKVSGFAKVDVLSGEVKKYLYGEQSYGGEPLFLPREGAEAEDDGHILAF 543
           QFSYLALAEPWPKVSGFAKVD+ +GE+KKYLYGEQ YGGEPLFLPREGAEAEDDGHILAF
Sbjct: 482 QFSYLALAEPWPKVSGFAKVDLFTGEIKKYLYGEQRYGGEPLFLPREGAEAEDDGHILAF 541

Query: 544 VHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISCKDLQKQIR 594
           VHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFIS +DLQKQIR
Sbjct: 542 VHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSEDLQKQIR 591

BLAST of CSPI04G07560 vs. NCBI nr
Match: gi|408794955|gb|AFU91491.1| (9-cis-epoxycarotenoid dioxygenase [Momordica charantia])

HSP 1 Score: 1023.5 bits (2645), Expect = 1.5e-295
Identity = 504/594 (84.85%), Postives = 543/594 (91.41%), Query Frame = 1

Query: 1   MASSSSS-FLQSPFSSSMPD--KKLSNYTTNSLLSVSSSLHTPSIIQIPKHSHTTFPSPL 60
           MAS+ SS +L+S FS SMP+     SNY  N    VS SLHTPSI+QIPKHS T  P PL
Sbjct: 1   MASAPSSPWLKSGFSPSMPELLSVNSNYVKNR---VSCSLHTPSIVQIPKHSQTFRPPPL 60

Query: 61  QTSIPKPPPLTTTPPVSGSQDWNFLQRAAAMALDAVENALISAERKHSLPKTADPAVQIA 120
           +T + KP     +P  S  +DWNFLQRAA+MA+DAVENAL+SAERK  LPKTADP VQIA
Sbjct: 61  KTPVEKP----VSPSTSAPEDWNFLQRAASMAIDAVENALLSAERKRPLPKTADPEVQIA 120

Query: 121 GNFAPVPEQPVRKGLPVIGKVPDHIRGVYVRNGANPLHEPLSGHHLFDGDGMVHAVEFSE 180
           GNFAPVPEQPVRK LPV G++P+ IRGVYVRNGANPLHEP +GHHLFDGDGMVHAVEFS+
Sbjct: 121 GNFAPVPEQPVRKALPVTGRIPECIRGVYVRNGANPLHEPAAGHHLFDGDGMVHAVEFSD 180

Query: 181 AGGVSYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHNHG 240
            GGVSYACRFTETQRLVQERA+GRPVFPKAIGELHGHSGIARL+LFYARGLFGLVDH+HG
Sbjct: 181 GGGVSYACRFTETQRLVQERAHGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHG 240

Query: 241 IGVANAGLVYFNGRLLAMSEDDLPYQIRVTPAGDLKTVGRFNFDGQLESTMIAHPKLDPV 300
           IGVANAGLVYFNGRLLAMSEDDLPYQIRVTPAGDLKT+GRF+FDGQL+STMIAHPKLDPV
Sbjct: 241 IGVANAGLVYFNGRLLAMSEDDLPYQIRVTPAGDLKTIGRFDFDGQLKSTMIAHPKLDPV 300

Query: 301 SGEMFALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKYVVIPDQQVV 360
           SGE+FA+SYDVIQKP+LKYFKFSPEGEKSPDVE+PLPQPTMMHDFAITEK+VVIPDQQVV
Sbjct: 301 SGELFAISYDVIQKPHLKYFKFSPEGEKSPDVEVPLPQPTMMHDFAITEKFVVIPDQQVV 360

Query: 361 FKLPEMIRGGSPVVYDKEKTSRFGILDKNATDANAIKWIEAPDCFCFHLWNAWEEPETNE 420
           FKLPEMIRGGSPV+YDK K SRFGIL KNATDA  IKWIEAPDCFCFHLWNAWEEPETNE
Sbjct: 361 FKLPEMIRGGSPVIYDKNKISRFGILPKNATDAGDIKWIEAPDCFCFHLWNAWEEPETNE 420

Query: 421 VVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIITETEQVNLEAGMVNRN 480
           VVVIGSCMTPPDSIFNECEENLKSVLSEIRLNL+TGKSTRRPII ETEQVNLEAGMVNRN
Sbjct: 421 VVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLTTGKSTRRPIIPETEQVNLEAGMVNRN 480

Query: 481 LLGRKTQFSYLALAEPWPKVSGFAKVDVLSGEVKKYLYGEQSYGGEPLFLPREGAEAEDD 540
            LGRKTQF+YLALAEPWPKVSGFAKVD+ +GE++KY+YGEQ YGGEPLFLPREGAEAEDD
Sbjct: 481 RLGRKTQFAYLALAEPWPKVSGFAKVDLSTGEIRKYIYGEQRYGGEPLFLPREGAEAEDD 540

Query: 541 GHILAFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISCKDLQKQ 592
           GHILAFVHDEKEWKSELQIVNA+TL+LEA+VKLPSRVPYGFHGTFI  K LQKQ
Sbjct: 541 GHILAFVHDEKEWKSELQIVNAITLKLEASVKLPSRVPYGFHGTFIGSKGLQKQ 587

BLAST of CSPI04G07560 vs. NCBI nr
Match: gi|1009109982|ref|XP_015893126.1| (PREDICTED: 9-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic-like [Ziziphus jujuba])

HSP 1 Score: 915.2 bits (2364), Expect = 5.8e-263
Identity = 449/588 (76.36%), Postives = 510/588 (86.73%), Query Frame = 1

Query: 6   SSFLQSPFSSSMPDKKLSNYTTNSLLSVSSSLHTPSIIQIPKHSHTTF-PSPLQTSIPKP 65
           +S +    SSS P  +L N       +++ SL TPS+I+IPK S +T+ PS   T   K 
Sbjct: 2   ASTISCSSSSSSPFLELGNGKKRRP-NINCSLQTPSVIRIPKQSPSTYQPSNTTTIKEKS 61

Query: 66  PPLTTTPPVSGSQDWNFLQRAAAMALDAVENALISAERKHSLPKTADPAVQIAGNFAPVP 125
            P + +      Q WNFLQ+AAAMAL AVE+AL+  ERKH LPKTADP+VQIAGNFAPV 
Sbjct: 62  TPNSVSDKPVPFQQWNFLQKAAAMALQAVESALVEHERKHPLPKTADPSVQIAGNFAPVQ 121

Query: 126 EQPVRKGLPVIGKVPDHIRGVYVRNGANPLHEPLSGHHLFDGDGMVHAVEFSEAGGVSYA 185
           EQPV+  LPV GK+P  I GVYVRNGANP +EP++GHH FDGDGMVHAV+F   G  SY+
Sbjct: 122 EQPVQHSLPVTGKIPKCIEGVYVRNGANPHYEPVAGHHFFDGDGMVHAVQFKN-GTASYS 181

Query: 186 CRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHNHGIGVANAG 245
           CRFTET RLVQERA+GRPVFPKAIGELHGHSGIARL+LFYARG+FGLVD NHG+GVANAG
Sbjct: 182 CRFTETHRLVQERAFGRPVFPKAIGELHGHSGIARLLLFYARGIFGLVDSNHGMGVANAG 241

Query: 246 LVYFNGRLLAMSEDDLPYQIRVTPAGDLKTVGRFNFDGQLESTMIAHPKLDPVSGEMFAL 305
           LVYFNGRLLAMSEDDLPY +R+TP+GDLKTVGR++F+GQL+STMIAHPKLDP +GE+FAL
Sbjct: 242 LVYFNGRLLAMSEDDLPYHVRITPSGDLKTVGRYDFNGQLKSTMIAHPKLDPETGELFAL 301

Query: 306 SYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKYVVIPDQQVVFKLPEMI 365
           SYDV+Q+PYLKYF FSP G+KS DVEIPL QPTMMHDFAITE +VVIPDQQVVFKLPEMI
Sbjct: 302 SYDVVQRPYLKYFHFSPSGKKSTDVEIPLSQPTMMHDFAITENFVVIPDQQVVFKLPEMI 361

Query: 366 RGGSPVVYDKEKTSRFGILDKNATDANAIKWIEAPDCFCFHLWNAWEEPETNEVVVIGSC 425
           RGGSPV+YD+EK +RFGILDKNATDA+ IKWIEAPDCFCFHLWNAWEEPE +EVVVIGSC
Sbjct: 362 RGGSPVIYDREKMARFGILDKNATDASGIKWIEAPDCFCFHLWNAWEEPENDEVVVIGSC 421

Query: 426 MTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIITETEQVNLEAGMVNRNLLGRKTQ 485
           MTPPDSIFNECEENLKSVLSEIRLNL TGKSTRRPII+E EQVNLEAGMVNRN LGRKT+
Sbjct: 422 MTPPDSIFNECEENLKSVLSEIRLNLKTGKSTRRPIISEQEQVNLEAGMVNRNRLGRKTR 481

Query: 486 FSYLALAEPWPKVSGFAKVDVLSGEVKKYLYGEQSYGGEPLFLPRE-GAEAEDDGHILAF 545
           F+YLALAEPWPKVSGFAKVD+ +GEV K++YGEQ +GGEPLFLP++  +E EDDG+ILAF
Sbjct: 482 FAYLALAEPWPKVSGFAKVDLFTGEVNKFMYGEQRFGGEPLFLPKDPNSENEDDGYILAF 541

Query: 546 VHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISCKDLQKQ 592
           VHDEKEWKSELQIVNAMT++LEAT+KLPSRVPYGFHGTFIS KDL+KQ
Sbjct: 542 VHDEKEWKSELQIVNAMTMKLEATIKLPSRVPYGFHGTFISSKDLEKQ 587

BLAST of CSPI04G07560 vs. NCBI nr
Match: gi|590611709|ref|XP_007022180.1| (Nine-cis-epoxycarotenoid dioxygenase 3 [Theobroma cacao])

HSP 1 Score: 913.3 bits (2359), Expect = 2.2e-262
Identity = 452/593 (76.22%), Postives = 507/593 (85.50%), Query Frame = 1

Query: 2   ASSSSSFLQSPFSSSMPDKKLSNYTTNSLLSVSSSLHTPSIIQIPKHSHTTFPSPLQTSI 61
           ++SSS+      SSS+P K+ +        ++S SL TPSI+  PK S T  PSP   S 
Sbjct: 26  STSSSAPDLGSISSSIPFKRPTIKP-----NISCSLQTPSILHFPKQSPTYPPSPTPASS 85

Query: 62  P--KPPPLTTTPPVSGSQDWNFLQRAAAMALDAVENALISAERKHSLPKTADPAVQIAGN 121
           P  K P  T++     SQ WN  QRAAAMALD VENAL+S ER H LPKTADP VQI+GN
Sbjct: 86  PTTKHPKNTSSAEKPQSQQWNPFQRAAAMALDVVENALVSHERNHPLPKTADPRVQISGN 145

Query: 122 FAPVPEQPVRKGLPVIGKVPDHIRGVYVRNGANPLHEPLSGHHLFDGDGMVHAVEFSEAG 181
           FAPVPEQP++  LPV G +P+ I+GVYVRNGANPLHEP++GHH FDGDGMVHAV+F   G
Sbjct: 146 FAPVPEQPIKHRLPVTGTIPECIQGVYVRNGANPLHEPVAGHHFFDGDGMVHAVQFKN-G 205

Query: 182 GVSYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHNHGIG 241
             SY CRFTET RLVQERA+GRPVFPKAIGELHGHSGIARL+LFYARGLFGLVD +HG G
Sbjct: 206 SASYGCRFTETSRLVQERAFGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDPSHGTG 265

Query: 242 VANAGLVYFNGRLLAMSEDDLPYQIRVTPAGDLKTVGRFNFDGQLESTMIAHPKLDPVSG 301
           VANAGLVYFNG LLAMSEDDLPY +R+TP+GDLKTVGR++FDGQL+STMIAHPK+DPV+G
Sbjct: 266 VANAGLVYFNGHLLAMSEDDLPYHVRITPSGDLKTVGRYDFDGQLKSTMIAHPKVDPVTG 325

Query: 302 EMFALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKYVVIPDQQVVFK 361
           E FALSYDVIQKPYLKYF FS +G+KSPDVEIP+  PTMMHDFAITE +VVIPDQQVVFK
Sbjct: 326 EFFALSYDVIQKPYLKYFHFSADGKKSPDVEIPVESPTMMHDFAITENFVVIPDQQVVFK 385

Query: 362 LPEMIRGGSPVVYDKEKTSRFGILDKNATDANAIKWIEAPDCFCFHLWNAWEEPETNEVV 421
           LPEMI GGSPVVYDK K SRFGILDKNATD + I W+EAPDCFCFHLWNAWEEP+T+EVV
Sbjct: 386 LPEMIHGGSPVVYDKNKMSRFGILDKNATDDSGISWVEAPDCFCFHLWNAWEEPQTDEVV 445

Query: 422 VIGSCMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIITETEQVNLEAGMVNRNLL 481
           VIGSCMTPPDSIFNEC+E+LKSVLSEIRLNL TGKSTRRPII+E+EQVNLEAGMVNRNLL
Sbjct: 446 VIGSCMTPPDSIFNECDESLKSVLSEIRLNLKTGKSTRRPIISESEQVNLEAGMVNRNLL 505

Query: 482 GRKTQFSYLALAEPWPKVSGFAKVDVLSGEVKKYLYGEQSYGGEPLFLPRE-GAEAEDDG 541
           GRKT+F+YLALAEPWPKVSGFAKVD+ +GEVKKY+YG+Q YGGEPLF PR   +E EDDG
Sbjct: 506 GRKTRFAYLALAEPWPKVSGFAKVDLSTGEVKKYIYGDQRYGGEPLFFPRNPNSENEDDG 565

Query: 542 HILAFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISCKDLQKQ 592
           +ILAFVHDEK W+SELQIVNAM L+LEATVKLPSRVPYGFHGTFIS KDL+KQ
Sbjct: 566 YILAFVHDEKTWQSELQIVNAMNLQLEATVKLPSRVPYGFHGTFISSKDLEKQ 612