Cp4.1LG00g04890 (gene) Cucurbita pepo (Zucchini)

BLAST of Cp4.1LG00g04890 vs. Swiss-Prot
Match: SYLC_ARATH (Leucine--tRNA ligase, cytoplasmic OS=Arabidopsis thaliana GN=At1g09620 PE=1 SV=1)

HSP 1 Score: 1095.1 bits (2831), Expect = 0.0e+00
Identity = 522/708 (73.73%), Postives = 612/708 (86.44%), Query Frame = 1

Query: 1    MLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRAKYGVKDEWVLPYDIVPIIDIPE 60
            MLTILT+KGTGIVTSVPSDAPDDYMA+ DL  KPAL+ KYGVK EW LP +I+PII+IPE
Sbjct: 381  MLTILTNKGTGIVTSVPSDAPDDYMALQDLIKKPALQDKYGVKTEW-LPTEIIPIINIPE 440

Query: 61   FGDRAAEKVCVDLKIKSQNEKDKLAEAKRLTYLKGFTDGTLIVGEFSGRKVQEAKPMIRS 120
            FGD+AAEKVC+DLKIKSQN+K+KLAEAKRLTYLKGFT+GT+++GEF GRKVQE KP+I++
Sbjct: 441  FGDKAAEKVCLDLKIKSQNDKEKLAEAKRLTYLKGFTEGTMLIGEFFGRKVQEIKPIIKT 500

Query: 121  KLIETGQAIPYSEPEKRVTSRSGDECIVALTDQWYITYGESEWKKLAEDCLANMNLFSDE 180
            KLIETG+AI YSEPEK V SRSGDEC+VALTDQWYITYGESEW+K+AE+CL+ MNL+SDE
Sbjct: 501  KLIETGEAIIYSEPEKPVMSRSGDECVVALTDQWYITYGESEWRKIAEECLSKMNLYSDE 560

Query: 181  TRHGFQHTLSWLNQWACSRSFGLGTRIPWDEQFLVESLSDSTIYMAYYTIAHFLHNGDLY 240
            TRHGF+HTLSWLNQWACSRSFGLGTRIPWDEQFLVESLSDS++YMAYYT+AH  H+GD+Y
Sbjct: 561  TRHGFEHTLSWLNQWACSRSFGLGTRIPWDEQFLVESLSDSSLYMAYYTVAHIFHDGDMY 620

Query: 241  ASDASAIKPEQMTDEVWDFIFCGAAEPKSTGISQSVLNKMKQEFEYWYPFDLRVSGKDLI 300
                S I+P+QM DEVW+++FC    PKS+ I  +VL++MKQEF+YWYP DLRVSGKDLI
Sbjct: 621  KGSKSLIRPQQMNDEVWEYLFCDGPYPKSSDIPSAVLSEMKQEFDYWYPLDLRVSGKDLI 680

Query: 301  QNHLTFCIYNHAAIMPRHHWPRGFRCNGHIMLNSEKMSKSTGNFRTLRQAIEEFSADATR 360
            QNHLTF IYNH A+M   +WPRG RCNGHIMLNSEKMSKSTGNFRTLRQ+IEEFSA  TR
Sbjct: 681  QNHLTFFIYNHTALMANRNWPRGIRCNGHIMLNSEKMSKSTGNFRTLRQSIEEFSATGTR 740

Query: 361  FSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDILQADSASFLRTGPPSTYADRVFE 420
            F LADAGDGVDDANF FETANAAILRLTKE+ WME++L  +S+  LRTGPPSTYAD+VFE
Sbjct: 741  FCLADAGDGVDDANFAFETANAAILRLTKELTWMEEVLDVESS--LRTGPPSTYADKVFE 800

Query: 421  NEINIAVRTTEKNYKDYMFREALKTGFYDLQAARDEYRFSCGAGGMNRDLVFRFMDVQTR 480
            N++NIA+R TE+ YKD +FREALK GFYDLQAARDEYR SCG GGM+ DL+ +FMDVQTR
Sbjct: 801  NDMNIALRLTERAYKDCLFREALKNGFYDLQAARDEYRLSCGTGGMHHDLLLKFMDVQTR 860

Query: 481  LITPICPHYAEYVWREMLKKEGFVINSGWPLADSPDLTLKIANKYLQDSIVLMRKLLQKQ 540
            LI PICPH+A+YVWR++L KEG V+ +GWP ++ PDL LK ANKYLQDSIVLMRKLLQKQ
Sbjct: 861  LIVPICPHFADYVWRKVLNKEGCVLTAGWPPSNEPDLVLKSANKYLQDSIVLMRKLLQKQ 920

Query: 541  LLGSKKGNKKGAPVTMVIEDKKLTGLVYVNEQFDGWKAECLRILRSKFDPTRRTFAPDSE 600
            L GSKKG KKGA VT V E  KL GLVYVNEQFDGW+A CLRIL+S+FD    +F PD+E
Sbjct: 921  LSGSKKGAKKGAQVTAVPEG-KLKGLVYVNEQFDGWRAHCLRILQSRFDQQTCSFPPDTE 980

Query: 601  IMEALQNSSVGQAADFKKTQKQCMPFLRFKKDEAAALGVQALDLKLPFGEMDVLRENLEL 660
            ++  L  + + +  + K  QK CMPFL+FKKDEA ++G QAL+L+LPFGE++VL+ N +L
Sbjct: 981  MLAELSATLLQEGKNLKAIQKVCMPFLKFKKDEAISIGTQALNLRLPFGEIEVLQSNKDL 1040

Query: 661  IKRQIGLEEVEILCASDPDAVAKAGALASLLTQNPPSPGNPTAIFLTS 709
            I+RQ+GLEEVEI  ASDPD V+ AG  ASLLTQNPPSPG+PTAIF+TS
Sbjct: 1041 IRRQLGLEEVEIYSASDPDDVSIAGPHASLLTQNPPSPGSPTAIFVTS 1084

BLAST of Cp4.1LG00g04890 vs. Swiss-Prot
Match: SYLC_PONAB (Leucine--tRNA ligase, cytoplasmic OS=Pongo abelii GN=LARS PE=2 SV=1)

HSP 1 Score: 664.1 bits (1712), Expect = 1.7e-189
Identity = 328/683 (48.02%), Postives = 461/683 (67.50%), Query Frame = 1

Query: 1    MLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRAKYGVKDEWVLPYDIVPIIDIPE 60
            MLTI  DKGTG+VTSVPSD+PDD  A+ DLK K ALRAKYG++D+ VLP++ VP+I+IP 
Sbjct: 377  MLTIKEDKGTGVVTSVPSDSPDDIAALRDLKKKQALRAKYGIRDDMVLPFEPVPVIEIPG 436

Query: 61   FGDRAAEKVCVDLKIKSQNEKDKLAEAKRLTYLKGFTDGTLIVGEFSGRKVQEAKPMIRS 120
            FG+ +A  +C +LKI+SQN+++KLAEAK   YLKGF +G ++V  F G+KVQ+ K  I+ 
Sbjct: 437  FGNLSAVTICDELKIQSQNDREKLAEAKEKIYLKGFYEGIMLVDGFKGQKVQDVKKTIQK 496

Query: 121  KLIETGQAIPYSEPEKRVTSRSGDECIVALTDQWYITYGESEWKKLAEDCLANMNLFSDE 180
            K+I+ G A+ Y EPEK+V SRS DEC+VAL DQWY+ YGE  WKK    CL N+  F +E
Sbjct: 497  KMIDAGDALIYMEPEKQVMSRSSDECVVALCDQWYLDYGEENWKKQTSQCLKNLETFCEE 556

Query: 181  TRHGFQHTLSWLNQWACSRSFGLGTRIPWDEQFLVESLSDSTIYMAYYTIAHFLHNGDLY 240
            TR  F+ TL WL + ACSR++GLGT +PWDEQ+L+ESLSDSTIYMA+YT+AH L  G+L+
Sbjct: 557  TRRNFEATLGWLQEHACSRTYGLGTHLPWDEQWLIESLSDSTIYMAFYTVAHLLQGGNLH 616

Query: 241  --ASDASAIKPEQMTDEVWDFIFCGAAEPKSTGISQSVLNKMKQEFEYWYPFDLRVSGKD 300
              A     I+P+QMT EVWD++F   A    T I++  L+++KQEFE+WYP DLRVSGKD
Sbjct: 617  GQAESPLGIRPQQMTKEVWDYVFFKEAPFPKTQIAKEKLDQLKQEFEFWYPVDLRVSGKD 676

Query: 301  LIQNHLTFCIYNHAAIMPRH--HWPRGFRCNGHIMLNSEKMSKSTGNFRTLRQAIEEFSA 360
            L+ NHL++ +YNH A+ P     WP   R NGH++LNSEKMSKSTGNF TL QAI++FSA
Sbjct: 677  LVPNHLSYYLYNHVAMWPEQSDKWPTAVRANGHLLLNSEKMSKSTGNFLTLTQAIDKFSA 736

Query: 361  DATRFSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDILQADSASFLRTGPPSTYAD 420
            D  R +LADAGD V+DANFV   A+A ILRL   + W+++++   +   LR+GP +T+ D
Sbjct: 737  DGMRLALADAGDTVEDANFVEAMADAGILRLYTWVEWVKEMVA--NWDSLRSGPANTFND 796

Query: 421  RVFENEINIAVRTTEKNYKDYMFREALKTGFYDLQAARDEYRFSCGAGGMNRDLVFRFMD 480
            RVF +E+N  +  T++NY+  MF+EALKTGF++ QAA+D+YR      GM+R+LVFRF++
Sbjct: 797  RVFASELNAGIIKTDQNYEKMMFKEALKTGFFEFQAAKDKYR-ELAVEGMHRELVFRFIE 856

Query: 481  VQTRLITPICPHYAEYVWREMLKKEGFVINSGWPLADSPDLTLKIANKYLQDSIVLMRKL 540
            VQT L+ P CPH  E++W  +L K   ++N+ WP+A   D  L  +++YL +    +R  
Sbjct: 857  VQTLLLAPFCPHLCEHIW-TLLGKPDSIMNASWPVAGPVDEVLIHSSQYLMEVTHDLRLR 916

Query: 541  LQKQLLGSKKGNKKGAPVTMVIEDKKLTGLVYVNEQFDGWKAECLRILRSKFDPTRRTFA 600
            L+  ++ +K       P+      K     +YV + +  W+   L +LR  F+   R   
Sbjct: 917  LKNYMMPAKGKKTDKQPL-----QKPSHCTIYVAKNYPPWQHTTLSVLRKHFEANNRKL- 976

Query: 601  PDSEIMEALQNSSVGQAADFKKTQKQCMPFLRFKKDEAAALGVQALDLKLPFGEMDVLRE 660
            PD++++     S +G   + KK  K+ MPF+   K+    +G + LDL+L F E  VL E
Sbjct: 977  PDNKVIA----SELGSMPELKKYMKKVMPFVAMIKENLEKMGPRILDLQLEFDEKAVLME 1036

Query: 661  NLELIKRQIGLEEVEILCASDPD 680
            N+  +   + LE +E+  AS+ +
Sbjct: 1037 NIVYLTNSLELEHIEVKFASEAE 1045

BLAST of Cp4.1LG00g04890 vs. Swiss-Prot
Match: SYLC_HUMAN (Leucine--tRNA ligase, cytoplasmic OS=Homo sapiens GN=LARS PE=1 SV=2)

HSP 1 Score: 660.6 bits (1703), Expect = 1.9e-188
Identity = 327/683 (47.88%), Postives = 460/683 (67.35%), Query Frame = 1

Query: 1    MLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRAKYGVKDEWVLPYDIVPIIDIPE 60
            MLTI  DKGTG+VTSVPSD+PDD  A+ DLK K ALRAKYG++D+ VLP++ VP+I+IP 
Sbjct: 377  MLTIKEDKGTGVVTSVPSDSPDDIAALRDLKKKQALRAKYGIRDDMVLPFEPVPVIEIPG 436

Query: 61   FGDRAAEKVCVDLKIKSQNEKDKLAEAKRLTYLKGFTDGTLIVGEFSGRKVQEAKPMIRS 120
            FG+ +A  +C +LKI+SQN+++KLAEAK   YLKGF +G ++V  F G+KVQ+ K  I+ 
Sbjct: 437  FGNLSAVTICDELKIQSQNDREKLAEAKEKIYLKGFYEGIMLVDGFKGQKVQDVKKTIQK 496

Query: 121  KLIETGQAIPYSEPEKRVTSRSGDECIVALTDQWYITYGESEWKKLAEDCLANMNLFSDE 180
            K+I+ G A+ Y EPEK+V SRS DEC+VAL DQWY+ YGE  WKK    CL N+  F +E
Sbjct: 497  KMIDAGDALIYMEPEKQVMSRSSDECVVALCDQWYLDYGEENWKKQTSQCLKNLETFCEE 556

Query: 181  TRHGFQHTLSWLNQWACSRSFGLGTRIPWDEQFLVESLSDSTIYMAYYTIAHFLHNGDLY 240
            TR  F+ TL WL + ACSR++GLGT +PWDEQ+L+ESLSDSTIYMA+YT+AH L  G+L+
Sbjct: 557  TRRNFEATLGWLQEHACSRTYGLGTHLPWDEQWLIESLSDSTIYMAFYTVAHLLQGGNLH 616

Query: 241  --ASDASAIKPEQMTDEVWDFIFCGAAEPKSTGISQSVLNKMKQEFEYWYPFDLRVSGKD 300
              A     I+P+QMT EVWD++F   A    T I++  L+++KQEFE+WYP DLRVSGKD
Sbjct: 617  GQAESPLGIRPQQMTKEVWDYVFFKEAPFPKTQIAKEKLDQLKQEFEFWYPVDLRVSGKD 676

Query: 301  LIQNHLTFCIYNHAAIMPRH--HWPRGFRCNGHIMLNSEKMSKSTGNFRTLRQAIEEFSA 360
            L+ NHL++ +YNH A+ P     WP   R NGH++LNSEKMSKSTGNF TL QAI++FSA
Sbjct: 677  LVPNHLSYYLYNHVAMWPEQSDKWPTAVRANGHLLLNSEKMSKSTGNFLTLTQAIDKFSA 736

Query: 361  DATRFSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDILQADSASFLRTGPPSTYAD 420
            D  R +LADAGD V+DANFV   A+A ILRL   + W+++++   +   LR+GP ST+ D
Sbjct: 737  DGMRLALADAGDTVEDANFVEAMADAGILRLYTWVEWVKEMVA--NWDSLRSGPASTFND 796

Query: 421  RVFENEINIAVRTTEKNYKDYMFREALKTGFYDLQAARDEYRFSCGAGGMNRDLVFRFMD 480
            RVF +E+N  +  T++NY+  MF+EALKTGF++ QAA+D+YR      GM+R+LVFRF++
Sbjct: 797  RVFASELNAGIIKTDQNYEKMMFKEALKTGFFEFQAAKDKYR-ELAVEGMHRELVFRFIE 856

Query: 481  VQTRLITPICPHYAEYVWREMLKKEGFVINSGWPLADSPDLTLKIANKYLQDSIVLMRKL 540
            VQT L+ P CPH  E++W  +L K   ++N+ WP+A   +  L  +++YL +    +R  
Sbjct: 857  VQTLLLAPFCPHLCEHIW-TLLGKPDSIMNASWPVAGPVNEVLIHSSQYLMEVTHDLRLR 916

Query: 541  LQKQLLGSKKGNKKGAPVTMVIEDKKLTGLVYVNEQFDGWKAECLRILRSKFDPTRRTFA 600
            L+  ++ +K       P+      K     +YV + +  W+   L +LR  F+       
Sbjct: 917  LKNYMMPAKGKKTDKQPL-----QKPSHCTIYVAKNYPPWQHTTLSVLRKHFEANNGKL- 976

Query: 601  PDSEIMEALQNSSVGQAADFKKTQKQCMPFLRFKKDEAAALGVQALDLKLPFGEMDVLRE 660
            PD++++     S +G   + KK  K+ MPF+   K+    +G + LDL+L F E  VL E
Sbjct: 977  PDNKVIA----SELGSMPELKKYMKKVMPFVAMIKENLEKMGPRILDLQLEFDEKAVLME 1036

Query: 661  NLELIKRQIGLEEVEILCASDPD 680
            N+  +   + LE +E+  AS+ +
Sbjct: 1037 NIVYLTNSLELEHIEVKFASEAE 1045

BLAST of Cp4.1LG00g04890 vs. Swiss-Prot
Match: SYLC_MOUSE (Leucine--tRNA ligase, cytoplasmic OS=Mus musculus GN=Lars PE=1 SV=2)

HSP 1 Score: 645.6 bits (1664), Expect = 6.4e-184
Identity = 328/711 (46.13%), Postives = 460/711 (64.70%), Query Frame = 1

Query: 1    MLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRAKYGVKDEWVLPYDIVPIIDIPE 60
            MLTI  DKGTG+VTSVPSD+PDD  A+ DLK K ALR K+G++D+ VLP++ VP+++IP 
Sbjct: 379  MLTIKEDKGTGVVTSVPSDSPDDLAALRDLKKKQALRTKFGIRDDMVLPFEPVPVLEIPG 438

Query: 61   FGDRAAEKVCVDLKIKSQNEKDKLAEAKRLTYLKGFTDGTLIVGEFSGRKVQEAKPMIRS 120
             G+  A  VC +LKI+SQN+++KLAEAK   YL+GF DG ++V  F G+K+Q  K  I+ 
Sbjct: 439  IGNLPAVTVCDELKIQSQNDREKLAEAKEKLYLRGFYDGVMLVDGFKGQKIQHVKKTIQK 498

Query: 121  KLIETGQAIPYSEPEKRVTSRSGDECIVALTDQWYITYGESEWKKLAEDCLANMNLFSDE 180
             +I+ G A+ Y EPEK+V SRS DEC+VAL DQWY+ YG+  WKK    CL NM  F +E
Sbjct: 499  NMIDAGDALIYMEPEKQVMSRSADECVVALCDQWYLDYGDENWKKQTFQCLKNMETFCEE 558

Query: 181  TRHGFQHTLSWLNQWACSRSFGLGTRIPWDEQFLVESLSDSTIYMAYYTIAHFLHNGDL- 240
            +R  F+ +L WL + ACSR++GLGTR+PWDEQ+L+ESLSDSTIYMA+YT+AH L  GDL 
Sbjct: 559  SRKNFEASLDWLQEHACSRTYGLGTRLPWDEQWLIESLSDSTIYMAFYTVAHLLQGGDLN 618

Query: 241  -YASDASAIKPEQMTDEVWDFIFCGAAEPKSTGISQSVLNKMKQEFEYWYPFDLRVSGKD 300
              A     I+P+QMT +VWD++F   A    T I +  L+++KQEFE+WYP DLR SGKD
Sbjct: 619  GQAESPLGIRPQQMTKDVWDYVFFKDAPFPKTQIPKEKLDQLKQEFEFWYPVDLRASGKD 678

Query: 301  LIQNHLTFCIYNHAAIMPRH--HWPRGFRCNGHIMLNSEKMSKSTGNFRTLRQAIEEFSA 360
            LI NHL++ IYNH A+ P     WP   R NGH++LNSEKMSKSTGNF TL QA+++FSA
Sbjct: 679  LIPNHLSYYIYNHVAMWPEQSDKWPVSVRANGHLLLNSEKMSKSTGNFLTLSQAVDKFSA 738

Query: 361  DATRFSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDILQADSASFLRTGPPSTYAD 420
            D  R +LADAGD V+DANFV   A+A ILRL   + W++++L   S S LR+GP  ++ D
Sbjct: 739  DGMRLALADAGDTVEDANFVEAMADAGILRLYTWVEWVKEMLA--SCSSLRSGPADSFND 798

Query: 421  RVFENEINIAVRTTEKNYKDYMFREALKTGFYDLQAARDEYRFSCGAGGMNRDLVFRFMD 480
            RVF +E+N  +  T++NY+  MF+EALKTGF++ QAA+D+YR      GM+R+LVFRF++
Sbjct: 799  RVFASEMNAGIIKTDQNYEKMMFKEALKTGFFEFQAAKDKYR-ELATEGMHRELVFRFIE 858

Query: 481  VQTRLITPICPHYAEYVWREMLKKEGFVINSGWPLADSPDLTLKIANKYLQDSIVLMRKL 540
            VQT L+TP CPH  E++W  +L K   ++++ WP+A   D +L  +++YL +    +R  
Sbjct: 859  VQTILLTPFCPHLCEHIW-TLLGKPDSIMHASWPVAGPVDESLIRSSQYLMEVAHDLRLR 918

Query: 541  LQKQLLGSKKGNKKGAPVTMVIEDKKLTGLVYVNEQFDGWKAECLRILRSKFDPTRRTFA 600
            L+  ++ +K       P       +     +YV + +  W+   L  LRS F+       
Sbjct: 919  LKNYMMPAKGKKTDKQPA-----QRPSHCTIYVAKNYPVWQHITLTTLRSHFEANNGKL- 978

Query: 601  PDSEIMEALQNSSVGQAADFKKTQKQCMPFLRFKKDEAAALGVQALDLKLPFGEMDVLRE 660
            PD++++     S +G   + KK  K+ MPF+   K+     G + LDL+L F E  VL E
Sbjct: 979  PDNKVIA----SELGSLPELKKYMKKVMPFVAMIKENMEKKGPRVLDLELEFDEQAVLME 1038

Query: 661  NLELIKRQIGLEEVEILCASDPDAVAKAGALASLLTQNPPSPGNPTAIFLT 708
            N+  +   + LE +E+  AS+ +   +              PG P  +F T
Sbjct: 1039 NIVYLTNSLELEHIEVKFASEAEDKVREEC----------CPGKPLNVFRT 1065

BLAST of Cp4.1LG00g04890 vs. Swiss-Prot
Match: SYLC_CAEEL (Leucine--tRNA ligase OS=Caenorhabditis elegans GN=lrs-1 PE=3 SV=2)

HSP 1 Score: 638.6 bits (1646), Expect = 7.8e-182
Identity = 320/679 (47.13%), Postives = 442/679 (65.10%), Query Frame = 1

Query: 1    MLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRAKYGVKDEWVLPYDIVPIIDIPE 60
            MLTI  DKGTG+VTSVPSD+PDD+ A+ DLK K  LR KYG+ DE VLP++ VPII I  
Sbjct: 378  MLTIKDDKGTGVVTSVPSDSPDDFAALSDLKKKKPLREKYGLTDEMVLPFEPVPIIKIEG 437

Query: 61   FGDRAAEKVCVDLKIKSQNEKDKLAEAKRLTYLKGFTDGTLIVGEFSGRKVQEAKPMIRS 120
             GD AA ++C  LKI+SQNEKDKL EAK+  YLKGF DG ++VG+++G+K  + K +I+ 
Sbjct: 438  LGDLAAVEMCSRLKIESQNEKDKLEEAKKEVYLKGFYDGVMLVGKYAGKKTADVKKVIQD 497

Query: 121  KLIETGQAIPYSEPEKRVTSRSGDECIVALTDQWYITYGESEWKKLAEDCLANMNLFSDE 180
             L   G A  Y EPEK+V SRSGDEC+VAL DQWY+ YGE+EWK  A+  L  +  F+DE
Sbjct: 498  DLTAEGLATKYVEPEKKVMSRSGDECVVALCDQWYLNYGEAEWKAAAKKVLEPLRTFNDE 557

Query: 181  TRHGFQHTLSWLNQWACSRSFGLGTRIPWDEQFLVESLSDSTIYMAYYTIAHFLHNGDLY 240
            TR G + T+ WL+++ACSRS+GLGT++PWD Q+L+ESLSDSTIY AYYT+AH L  G   
Sbjct: 558  TRRGLETTVDWLHEYACSRSYGLGTKLPWDTQYLIESLSDSTIYNAYYTVAHLLQQGAFD 617

Query: 241  AS--DASAIKPEQMTDEVWDFIFCGAA-EPKSTGISQSVLNKMKQEFEYWYPFDLRVSGK 300
             S    + IK +QMTD  W ++F G   + K+  + +  L  +++EF YWYP D+R SGK
Sbjct: 618  GSVVGPAGIKADQMTDASWSYVFLGEIYDSKTMPVEEEKLKSLRKEFMYWYPIDMRASGK 677

Query: 301  DLIQNHLTFCIYNHAAIMP--RHHWPRGFRCNGHIMLNSEKMSKSTGNFRTLRQAIEEFS 360
            DLI NHLT+ ++NHAAI P     WP+G R NGH++LN+EKMSKSTGNF TL +AIE+FS
Sbjct: 678  DLIGNHLTYLLFNHAAIWPTDTSKWPKGIRANGHLLLNNEKMSKSTGNFMTLEEAIEKFS 737

Query: 361  ADATRFSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDILQADSASFLRTGPPSTYA 420
            AD  R SLADAGDG++DANFV+  A+AAILRL   I W++++++   A  LR      +A
Sbjct: 738  ADGMRLSLADAGDGLEDANFVYAMADAAILRLFTMIEWIKEMIEQRDAGLLRK-DAKKFA 797

Query: 421  DRVFENEINIAVRTTEKNYKDYMFREALKTGFYDLQAARDEYRFSCGA--GGMNRDLVFR 480
            DRVF NE+N  +  TE+NY+   F++ALKTGF++ QA RD YR  C      M+  LVFR
Sbjct: 798  DRVFANEMNSLIAATEQNYEATNFKDALKTGFFEYQAIRDTYRELCAGIDEPMSESLVFR 857

Query: 481  FMDVQTRLITPICPHYAEYVWREMLKKEGFVINSGWPLADSPDLTLKIANKYLQDSIVLM 540
            F++ Q  +++PICPH AEY+W ++L K+G ++N+ WP  D  D  L I  +++ +S+   
Sbjct: 858  FIETQMLILSPICPHIAEYIW-QLLGKDGLIVNAPWPTVDPVDEKLAIGARFITESLAEF 917

Query: 541  RKLLQKQLLGSKKGNKKGAPVTMVIEDKKLTGLVYVNEQFDGWKAECLRILRSKFDPTRR 600
            R  L+  +   KK  K+       I +     ++YV +++  W+   L IL  +      
Sbjct: 918  RARLKTYMTPKKKALKE-------IPEVPTEAVIYVAKEYPPWQKTILDILEKQAKANNG 977

Query: 601  TFAPDSEIMEALQNSSVGQAADFKKTQKQCMPFLRFKKDEAAALGVQALDLKLPFGEMDV 660
               PD++ +  L    +G+    KK  K+ MPF++  K+     GV AL    P  +  +
Sbjct: 978  AL-PDNKAISQL----IGKEESLKKFAKKAMPFVQMIKERFEQKGVSALASSSPVDQTSI 1037

Query: 661  LRENLELIKRQIGLEEVEI 673
            L EN++ I   + L+ V I
Sbjct: 1038 LNENIDFIMNALDLDRVTI 1042

BLAST of Cp4.1LG00g04890 vs. TrEMBL
Match: A0A0A0LV91_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G084310 PE=3 SV=1)

HSP 1 Score: 1338.6 bits (3463), Expect = 0.0e+00
Identity = 646/708 (91.24%), Postives = 682/708 (96.33%), Query Frame = 1

Query: 1    MLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRAKYGVKDEWVLPYDIVPIIDIPE 60
            MLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRAKYGVKDEWVLPYDIVPIIDIPE
Sbjct: 383  MLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRAKYGVKDEWVLPYDIVPIIDIPE 442

Query: 61   FGDRAAEKVCVDLKIKSQNEKDKLAEAKRLTYLKGFTDGTLIVGEFSGRKVQEAKPMIRS 120
            FGDRAAEKVC+DLKIKSQNEKDKLAEAKRLTYL+GFTDGTLIVGEF+GRKVQEAKP+IRS
Sbjct: 443  FGDRAAEKVCLDLKIKSQNEKDKLAEAKRLTYLRGFTDGTLIVGEFAGRKVQEAKPLIRS 502

Query: 121  KLIETGQAIPYSEPEKRVTSRSGDECIVALTDQWYITYGESEWKKLAEDCLANMNLFSDE 180
            +LIETGQAIPYSEPEKRV SRSGDECIVALTDQWYI YGESEWKKL+E+CLA+M++FSDE
Sbjct: 503  QLIETGQAIPYSEPEKRVMSRSGDECIVALTDQWYIIYGESEWKKLSEECLASMDMFSDE 562

Query: 181  TRHGFQHTLSWLNQWACSRSFGLGTRIPWDEQFLVESLSDSTIYMAYYTIAHFLHNGDLY 240
            TRHGF+HTL WLNQWACSRSFGLGTRIPWD+QFLVESLSDSTIYMAYYTIAH L NGDLY
Sbjct: 563  TRHGFEHTLGWLNQWACSRSFGLGTRIPWDKQFLVESLSDSTIYMAYYTIAHLLQNGDLY 622

Query: 241  ASDASAIKPEQMTDEVWDFIFCGAAEPKSTGISQSVLNKMKQEFEYWYPFDLRVSGKDLI 300
             S  SA+KPEQMTDEVWDF+FCGAAEPKSTGISQS+LNKMKQEFEYWYPFDLRVSGKDLI
Sbjct: 623  GSGDSAVKPEQMTDEVWDFVFCGAAEPKSTGISQSILNKMKQEFEYWYPFDLRVSGKDLI 682

Query: 301  QNHLTFCIYNHAAIMPRHHWPRGFRCNGHIMLNSEKMSKSTGNFRTLRQAIEEFSADATR 360
            QNHLTF IYNH AIMP+ HWPR FRCNGHIMLNSEKMSKSTGNFRTLR+AIEEFSADATR
Sbjct: 683  QNHLTFTIYNHTAIMPKRHWPRAFRCNGHIMLNSEKMSKSTGNFRTLREAIEEFSADATR 742

Query: 361  FSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDILQADSASFLRTGPPSTYADRVFE 420
            FSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDILQADS+SFLRTGPPSTYADRVFE
Sbjct: 743  FSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDILQADSSSFLRTGPPSTYADRVFE 802

Query: 421  NEINIAVRTTEKNYKDYMFREALKTGFYDLQAARDEYRFSCGAGGMNRDLVFRFMDVQTR 480
            NEINIAV+ TE+NYKDYMFREALKTGFYDLQAARDEYRFSCGAGGMNRDLVFRFMDVQTR
Sbjct: 803  NEINIAVKMTEQNYKDYMFREALKTGFYDLQAARDEYRFSCGAGGMNRDLVFRFMDVQTR 862

Query: 481  LITPICPHYAEYVWREMLKKEGFVINSGWPLADSPDLTLKIANKYLQDSIVLMRKLLQKQ 540
            LITPICPHYAE+VWR MLKK GFV+N+GWP ADSPDLTLK ANKYLQDSIVLMRKLLQKQ
Sbjct: 863  LITPICPHYAEHVWRNMLKKNGFVVNAGWPSADSPDLTLKSANKYLQDSIVLMRKLLQKQ 922

Query: 541  LLGSKKGNKKGAPVTMVIEDKKLTGLVYVNEQFDGWKAECLRILRSKFDPTRRTFAPDSE 600
            LLGSKKGNKKGAPVT V+EDKKLTGL+YVNEQFDGWKAECLRIL+SKFD T+RTFAPDSE
Sbjct: 923  LLGSKKGNKKGAPVTTVVEDKKLTGLIYVNEQFDGWKAECLRILQSKFDSTKRTFAPDSE 982

Query: 601  IMEALQNSSVGQAADFKKTQKQCMPFLRFKKDEAAALGVQALDLKLPFGEMDVLRENLEL 660
            IMEALQ SSVGQAADF++TQK CMPFLRFKKDEA +LGVQAL+L+LPFGEMDVL ENLEL
Sbjct: 983  IMEALQKSSVGQAADFRQTQKLCMPFLRFKKDEAVSLGVQALNLRLPFGEMDVLNENLEL 1042

Query: 661  IKRQIGLEEVEILCASDPDAVAKAGALASLLTQNPPSPGNPTAIFLTS 709
            I+RQIGLEEV+IL  SDP+A+AKAGALASLL QNPPSPGNPTAIFLTS
Sbjct: 1043 IRRQIGLEEVQILRVSDPNALAKAGALASLLKQNPPSPGNPTAIFLTS 1090

BLAST of Cp4.1LG00g04890 vs. TrEMBL
Match: A0A067LEE1_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_00261 PE=3 SV=1)

HSP 1 Score: 1257.3 bits (3252), Expect = 0.0e+00
Identity = 600/708 (84.75%), Postives = 663/708 (93.64%), Query Frame = 1

Query: 1    MLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRAKYGVKDEWVLPYDIVPIIDIPE 60
            MLTILTDKGTGIVTSVPSDAPDDYMA+HDLK+KPA RAKYGVKDEWVLP++IVPII+IP+
Sbjct: 381  MLTILTDKGTGIVTSVPSDAPDDYMALHDLKAKPAFRAKYGVKDEWVLPFEIVPIINIPD 440

Query: 61   FGDRAAEKVCVDLKIKSQNEKDKLAEAKRLTYLKGFTDGTLIVGEFSGRKVQEAKPMIRS 120
            FGD+AAEKVC+DLKIKSQNEKDKLAEAKRLTYL+GFTDGT++VGEF+GRKVQEAKP+IR+
Sbjct: 441  FGDKAAEKVCLDLKIKSQNEKDKLAEAKRLTYLRGFTDGTMLVGEFAGRKVQEAKPLIRA 500

Query: 121  KLIETGQAIPYSEPEKRVTSRSGDECIVALTDQWYITYGESEWKKLAEDCLANMNLFSDE 180
            KLIETG+AI YSEPEKRV SRSGDEC+VALTDQWYITYGE EWKKLAE+C +NMNL+SDE
Sbjct: 501  KLIETGEAIMYSEPEKRVVSRSGDECVVALTDQWYITYGEEEWKKLAEECFSNMNLYSDE 560

Query: 181  TRHGFQHTLSWLNQWACSRSFGLGTRIPWDEQFLVESLSDSTIYMAYYTIAHFLHNGDLY 240
            TRHGF+HTLSWLNQWACSRSFGLGTRIPWD++FLVESLSDSTIYMAYYT+AH LHN D+Y
Sbjct: 561  TRHGFEHTLSWLNQWACSRSFGLGTRIPWDKEFLVESLSDSTIYMAYYTVAHLLHNDDMY 620

Query: 241  ASD-ASAIKPEQMTDEVWDFIFCGAAEPKSTGISQSVLNKMKQEFEYWYPFDLRVSGKDL 300
             ++   ++KPEQMTDEVWDFI CG   PKS+ I  S+L+KMKQEFEYWYPFDLRVSGKDL
Sbjct: 621  GTNKPHSVKPEQMTDEVWDFIICGGPHPKSSDIPSSILDKMKQEFEYWYPFDLRVSGKDL 680

Query: 301  IQNHLTFCIYNHAAIMPRHHWPRGFRCNGHIMLNSEKMSKSTGNFRTLRQAIEEFSADAT 360
            IQNHLTFCIYNH AIM +HHWPRGFRCNGH+MLNSEKMSKSTGNFRT+RQAIEEFSADAT
Sbjct: 681  IQNHLTFCIYNHTAIMAKHHWPRGFRCNGHMMLNSEKMSKSTGNFRTVRQAIEEFSADAT 740

Query: 361  RFSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDILQADSASFLRTGPPSTYADRVF 420
            RFSLADAGDGVDDANFVFETANAAILRLTKEIAWME++L A+S+  LR GPPSTYADRVF
Sbjct: 741  RFSLADAGDGVDDANFVFETANAAILRLTKEIAWMEEVLAAESS--LRIGPPSTYADRVF 800

Query: 421  ENEINIAVRTTEKNYKDYMFREALKTGFYDLQAARDEYRFSCGAGGMNRDLVFRFMDVQT 480
            ENEINIAVR TE+NY+ YMFREALK+GFYDLQAARDEYRFSCG+GGMNRDLV+RFMDVQT
Sbjct: 801  ENEINIAVRMTEQNYRGYMFREALKSGFYDLQAARDEYRFSCGSGGMNRDLVWRFMDVQT 860

Query: 481  RLITPICPHYAEYVWREMLKKEGFVINSGWPLADSPDLTLKIANKYLQDSIVLMRKLLQK 540
            RLITPICPHYAEYVWRE+LKK+GFV+ +GWP+A SPDLTLK ANKYLQDSIVLMRKLLQK
Sbjct: 861  RLITPICPHYAEYVWRELLKKDGFVVKAGWPMAGSPDLTLKAANKYLQDSIVLMRKLLQK 920

Query: 541  QLLGSKKGNKKGAPVTMVIEDKKLTGLVYVNEQFDGWKAECLRILRSKFDPTRRTFAPDS 600
            QLLGSKKGNKKGAPV  + ED KLTGL+YVNEQFDGWKAECLRIL+SKFD   RT APD 
Sbjct: 921  QLLGSKKGNKKGAPVATLTED-KLTGLIYVNEQFDGWKAECLRILQSKFDHKNRTVAPDG 980

Query: 601  EIMEALQNSSVGQAADFKKTQKQCMPFLRFKKDEAAALGVQALDLKLPFGEMDVLRENLE 660
            EIM+AL+NSSVGQA +FK+TQK CMPFLRFKKDEA A+G QALDLKLPFGE++VL+ENL+
Sbjct: 981  EIMDALKNSSVGQATNFKQTQKLCMPFLRFKKDEAIAIGSQALDLKLPFGEIEVLKENLD 1040

Query: 661  LIKRQIGLEEVEILCASDPDAVAKAGALASLLTQNPPSPGNPTAIFLT 708
            LIKRQIGLEEVEIL A+DPDA+AKAG+L SLL QNPPSPGNPTAIFLT
Sbjct: 1041 LIKRQIGLEEVEILSAADPDALAKAGSLVSLLNQNPPSPGNPTAIFLT 1085

BLAST of Cp4.1LG00g04890 vs. TrEMBL
Match: A0A0D2SRK5_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_007G263900 PE=3 SV=1)

HSP 1 Score: 1222.6 bits (3162), Expect = 0.0e+00
Identity = 583/706 (82.58%), Postives = 645/706 (91.36%), Query Frame = 1

Query: 1    MLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRAKYGVKDEWVLPYDIVPIIDIPE 60
            MLTILTDKGTGIVTSVPSDAPDDYMA+ DLK+KPA RAK+GVKDEWVLP++IVPIIDIPE
Sbjct: 383  MLTILTDKGTGIVTSVPSDAPDDYMALQDLKAKPAFRAKFGVKDEWVLPFEIVPIIDIPE 442

Query: 61   FGDRAAEKVCVDLKIKSQNEKDKLAEAKRLTYLKGFTDGTLIVGEFSGRKVQEAKPMIRS 120
            +GDRAAEKVC+DLKIKSQNEKDKLAEAKRL YL+GFT+GT+IVGE++GR+VQEAKP++R+
Sbjct: 443  YGDRAAEKVCLDLKIKSQNEKDKLAEAKRLVYLRGFTEGTMIVGEYAGRRVQEAKPLLRT 502

Query: 121  KLIETGQAIPYSEPEKRVTSRSGDECIVALTDQWYITYGESEWKKLAEDCLANMNLFSDE 180
            KLIETGQAI YSEPEK+V SRSGDEC+VALTDQWYITYGE EWKKL+E+CL+NMNL+SDE
Sbjct: 503  KLIETGQAIIYSEPEKKVMSRSGDECVVALTDQWYITYGEPEWKKLSEECLSNMNLYSDE 562

Query: 181  TRHGFQHTLSWLNQWACSRSFGLGTRIPWDEQFLVESLSDSTIYMAYYTIAHFLHNGDLY 240
            TRHGF+HTL WLNQWACSRSFGLGTRIPWDE+FLVESLSDSTIYMAYYT+AH L NGD+Y
Sbjct: 563  TRHGFEHTLGWLNQWACSRSFGLGTRIPWDEEFLVESLSDSTIYMAYYTVAHLLQNGDMY 622

Query: 241  ASDASAIKPEQMTDEVWDFIFCGAAEPKSTGISQSVLNKMKQEFEYWYPFDLRVSGKDLI 300
               A  ++P QMTDEVW+F+FCG   PKS+ I  + LNKMKQEFEYWYPFDLRVSGKDLI
Sbjct: 623  GKSADLVQPAQMTDEVWEFLFCGGPYPKSSNIPAATLNKMKQEFEYWYPFDLRVSGKDLI 682

Query: 301  QNHLTFCIYNHAAIMPRHHWPRGFRCNGHIMLNSEKMSKSTGNFRTLRQAIEEFSADATR 360
            QNHLTFCIYNH AIM + HWPRGFRCNGHIMLNSEKMSKSTGNFRTLRQAIEEFSADATR
Sbjct: 683  QNHLTFCIYNHTAIMSKDHWPRGFRCNGHIMLNSEKMSKSTGNFRTLRQAIEEFSADATR 742

Query: 361  FSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDILQADSASFLRTGPPSTYADRVFE 420
            FSLADAGDGVDDANFVFETAN AILRLTKEIAWME+IL A+S+  LR GPPSTYADRVFE
Sbjct: 743  FSLADAGDGVDDANFVFETANTAILRLTKEIAWMEEILAAESS--LRIGPPSTYADRVFE 802

Query: 421  NEINIAVRTTEKNYKDYMFREALKTGFYDLQAARDEYRFSCGAGGMNRDLVFRFMDVQTR 480
            NEINIAV+ TEKNY+DYMFREALKTGFYDLQ ARDEYRFSCG+GGMNRDLV+RFMDVQTR
Sbjct: 803  NEINIAVKMTEKNYRDYMFREALKTGFYDLQTARDEYRFSCGSGGMNRDLVWRFMDVQTR 862

Query: 481  LITPICPHYAEYVWREMLKKEGFVINSGWPLADSPDLTLKIANKYLQDSIVLMRKLLQKQ 540
            LITP+CPHYAE+VWRE+LKK+GFVI +GWP A SPDL LK ANKYLQDSIVLMRKLL KQ
Sbjct: 863  LITPVCPHYAEFVWRELLKKDGFVIKAGWPSAVSPDLKLKSANKYLQDSIVLMRKLLNKQ 922

Query: 541  LLGSKKGNKKGAPVTMVIEDKKLTGLVYVNEQFDGWKAECLRILRSKFDPTRRTFAPDSE 600
            +LGSKK NKKGAP T + ED +L GL+YVNE+F+GW+AECL IL+SKFD   RTFA D E
Sbjct: 923  ILGSKKSNKKGAPATSLSED-RLKGLIYVNEKFEGWQAECLEILQSKFDSNARTFASDGE 982

Query: 601  IMEALQNSSVGQAADFKKTQKQCMPFLRFKKDEAAALGVQALDLKLPFGEMDVLRENLEL 660
            IM+ALQ S+VGQAADFKK QKQCMPFLRFKKDEA  +GVQALDLKLPFGE+DVL+ENL+L
Sbjct: 983  IMKALQESTVGQAADFKKIQKQCMPFLRFKKDEAIKIGVQALDLKLPFGEIDVLKENLDL 1042

Query: 661  IKRQIGLEEVEILCASDPDAVAKAGALASLLTQNPPSPGNPTAIFL 707
            IKRQ+GLEEVE+L  +D DA AKAG  ASLL QNPPSPGNPTAIFL
Sbjct: 1043 IKRQLGLEEVEVLSFTDSDASAKAGTHASLLNQNPPSPGNPTAIFL 1085

BLAST of Cp4.1LG00g04890 vs. TrEMBL
Match: M5Y947_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa000578mg PE=3 SV=1)

HSP 1 Score: 1218.8 bits (3152), Expect = 0.0e+00
Identity = 578/708 (81.64%), Postives = 647/708 (91.38%), Query Frame = 1

Query: 1    MLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRAKYGVKDEWVLPYDIVPIIDIPE 60
            MLT+LTDKGTGIVTSVP+D+PDDYMA+HDLK+KPALR KYGVKDEWV+P++I+PII+IPE
Sbjct: 382  MLTVLTDKGTGIVTSVPADSPDDYMALHDLKAKPALREKYGVKDEWVMPFEIIPIINIPE 441

Query: 61   FGDRAAEKVCVDLKIKSQNEKDKLAEAKRLTYLKGFTDGTLIVGEFSGRKVQEAKPMIRS 120
            FG++AAEKVC DLKIKSQNEKDKLAEAKRLTYLKGFT+GTLIVGEF+GRKVQ+ KP+IRS
Sbjct: 442  FGNKAAEKVCADLKIKSQNEKDKLAEAKRLTYLKGFTEGTLIVGEFNGRKVQDVKPLIRS 501

Query: 121  KLIETGQAIPYSEPEKRVTSRSGDECIVALTDQWYITYGESEWKKLAEDCLANMNLFSDE 180
            KLIE  +AI YSEPEKRV SRSGDEC+VALTDQWYITYGE EWKKLAE+CL++MNL+SDE
Sbjct: 502  KLIEANEAIVYSEPEKRVVSRSGDECVVALTDQWYITYGEPEWKKLAEECLSSMNLYSDE 561

Query: 181  TRHGFQHTLSWLNQWACSRSFGLGTRIPWDEQFLVESLSDSTIYMAYYTIAHFLHNGDLY 240
            TRHGF+HTL WLNQWACSRSFGLGTRIPWDE+FLVESLSDSTIYMAYYTIAHFLHNGD+Y
Sbjct: 562  TRHGFEHTLGWLNQWACSRSFGLGTRIPWDEEFLVESLSDSTIYMAYYTIAHFLHNGDMY 621

Query: 241  ASDASAIKPEQMTDEVWDFIFCGAAEPKSTGISQSVLNKMKQEFEYWYPFDLRVSGKDLI 300
             S  SAIKP QMTDEVW++IFC    P+S+ IS  +LNKMKQEFEYWYPFD+RVSGKDLI
Sbjct: 622  GSSKSAIKPGQMTDEVWEYIFCDGPYPESSDISSLILNKMKQEFEYWYPFDIRVSGKDLI 681

Query: 301  QNHLTFCIYNHAAIMPRHHWPRGFRCNGHIMLNSEKMSKSTGNFRTLRQAIEEFSADATR 360
            QNHLTFCIYNH AIMP+ HWPRGFRCNGHIMLNSEKMSKSTGNFRT+RQAIEEFSADATR
Sbjct: 682  QNHLTFCIYNHTAIMPKKHWPRGFRCNGHIMLNSEKMSKSTGNFRTIRQAIEEFSADATR 741

Query: 361  FSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDILQADSASFLRTGPPSTYADRVFE 420
            FSLADAGDGVDDANFVFETANAAILRLTKEIAWME++L  DS+  LR GPP+TYADRVF 
Sbjct: 742  FSLADAGDGVDDANFVFETANAAILRLTKEIAWMEEVLATDSS--LRIGPPTTYADRVFL 801

Query: 421  NEINIAVRTTEKNYKDYMFREALKTGFYDLQAARDEYRFSCGAGGMNRDLVFRFMDVQTR 480
            NEINIAV  TE+NY+DYMFR ALKTGFYDLQAARDEYRFSCG+GGMNR+LV RFMDVQTR
Sbjct: 802  NEINIAVNRTEQNYRDYMFRGALKTGFYDLQAARDEYRFSCGSGGMNRELVLRFMDVQTR 861

Query: 481  LITPICPHYAEYVWREMLKKEGFVINSGWPLADSPDLTLKIANKYLQDSIVLMRKLLQKQ 540
            LITPICPHYAEYVWRE+LKKEGFV+N+GWP+AD+PDLTL+ +NKYLQDSIVLMRKL +KQ
Sbjct: 862  LITPICPHYAEYVWRELLKKEGFVVNAGWPVADAPDLTLQSSNKYLQDSIVLMRKLYEKQ 921

Query: 541  LLGSKKGNKKGAPVTMVIEDKKL-TGLVYVNEQFDGWKAECLRILRSKFDPTRRTFAPDS 600
              GSKK NKKGAPVT V E+K+L  GL+YVNEQFD WKAECLRIL+S FD    TFAPD 
Sbjct: 922  RSGSKKANKKGAPVTAVTENKQLPIGLIYVNEQFDEWKAECLRILQSNFDRESCTFAPDR 981

Query: 601  EIMEALQNSSVGQAADFKKTQKQCMPFLRFKKDEAAALGVQALDLKLPFGEMDVLRENLE 660
             IMEALQ SS+GQ  DF++TQK CMPF++ KKD+A A+G QALDLKLPFGE+D+LRENL+
Sbjct: 982  VIMEALQRSSIGQTKDFRQTQKLCMPFMKMKKDQAVAIGAQALDLKLPFGEIDILRENLD 1041

Query: 661  LIKRQIGLEEVEILCASDPDAVAKAGALASLLTQNPPSPGNPTAIFLT 708
            LIKRQIGLEEVE+L ASDPDA+ KAG+L  L+ QNPPSPG+PTAIFL+
Sbjct: 1042 LIKRQIGLEEVEVLSASDPDALNKAGSLVKLVEQNPPSPGSPTAIFLS 1087

BLAST of Cp4.1LG00g04890 vs. TrEMBL
Match: W9R0H2_9ROSA (Leucine--tRNA ligase OS=Morus notabilis GN=L484_002340 PE=3 SV=1)

HSP 1 Score: 1216.8 bits (3147), Expect = 0.0e+00
Identity = 587/710 (82.68%), Postives = 645/710 (90.85%), Query Frame = 1

Query: 1    MLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRAKYGVKDEWVLPYDIVPIIDIPE 60
            MLTILTDKGTGIVTSVPSDAPDDYMA+HDLKSKPALRAKYGVKDEWVLP++IVPIIDIP 
Sbjct: 383  MLTILTDKGTGIVTSVPSDAPDDYMALHDLKSKPALRAKYGVKDEWVLPFEIVPIIDIPG 442

Query: 61   FGDRAAEKVCVDLKIKSQNEKDKLAEAKRLTYLKGFTDGTLIVGEFSGRKVQEAKPMIRS 120
            FGDRAAEKVCVDLKIKSQNEKDKLAEAKRLTYL+GFTDGT++VGEF+G+KVQE KP+IRS
Sbjct: 443  FGDRAAEKVCVDLKIKSQNEKDKLAEAKRLTYLRGFTDGTIVVGEFAGKKVQEVKPLIRS 502

Query: 121  KLIETGQAIPYSEPEKRVTSRSGDECIVALTDQWYITYGESEWKKLAEDCLANMNLFSDE 180
            KL+ETG+AI YSEPEKRV SRSGDEC+VALTDQWYITYGE EW+KLAE+CLANMNL+SDE
Sbjct: 503  KLLETGEAIMYSEPEKRVMSRSGDECVVALTDQWYITYGEPEWRKLAEECLANMNLYSDE 562

Query: 181  TRHGFQHTLSWLNQWACSRSFGLGTRIPWDEQFLVESLSDSTIYMAYYTIAHFLHNGDLY 240
            TRHGF+HTLSWLNQWACSRSFGLGTRIPWDEQFLVESLSDSTIYMAYYTIAH LHN D+Y
Sbjct: 563  TRHGFEHTLSWLNQWACSRSFGLGTRIPWDEQFLVESLSDSTIYMAYYTIAHLLHNEDMY 622

Query: 241  ASDASAIKPEQMTDEVWDFIFCGAAEPKSTGISQSVLNKMKQEFEYWYPFDLRVSGKDLI 300
             +  S I P+QMTDEVWDFIFCG   P S+ I  S+LNKMKQEFEYWYPFD+RVSGKDLI
Sbjct: 623  GTSRSPIAPDQMTDEVWDFIFCGGPYPNSSDIPSSILNKMKQEFEYWYPFDIRVSGKDLI 682

Query: 301  QNHLTFCIYNHAAIMPRHHWPRGFRCNGHIMLNSEKMSKSTGNFRTLRQAIEEFSADATR 360
            QNHLTF IYNH AIM + HWP GFR NGHIMLNSEKMSKSTGNFRT+RQAIEEFSADATR
Sbjct: 683  QNHLTFSIYNHTAIMAKQHWPCGFRANGHIMLNSEKMSKSTGNFRTIRQAIEEFSADATR 742

Query: 361  FSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDILQADSASFLRTGPPSTYADRVFE 420
            FSLADAGDGVDDANFVFETANAAILRLTKE+AWME++L ADS+  LR+G PSTYADRVF 
Sbjct: 743  FSLADAGDGVDDANFVFETANAAILRLTKELAWMEEVLAADSS--LRSGHPSTYADRVFA 802

Query: 421  NEINIAVRTTEKNYKDYMFREALKTGFYDLQAARDEYRFSCGAGGMNRDLVFRFMDVQTR 480
            NEINIAV TTE+NY ++MFREALKTGFYDLQAARDEYRFSCGAGGMNRDLV+RFMDVQTR
Sbjct: 803  NEINIAVTTTEQNYHNFMFREALKTGFYDLQAARDEYRFSCGAGGMNRDLVWRFMDVQTR 862

Query: 481  LITPICPHYAEYVWREMLKKEGFVINSGWPLADSPDLTLKIANKYLQDSIVLMRKLLQKQ 540
            LITPICPHYAEYVWRE LKKEGFV+N+GWP+A+ PDLTLK  NKYLQDSIVLMRKLLQKQ
Sbjct: 863  LITPICPHYAEYVWREHLKKEGFVVNAGWPVAEVPDLTLKKGNKYLQDSIVLMRKLLQKQ 922

Query: 541  LLGSKKGNKKGAPVTMVIEDKKL-TGLVYVNEQFDGWKAECLRILRSKFDPTRRTF--AP 600
             LGSKKGNKK APVT   ED KL  GL+YVNEQF+GWKAECL +LRS+FD + RTF  + 
Sbjct: 923  TLGSKKGNKKAAPVTTTTEDNKLVVGLIYVNEQFNGWKAECLEMLRSRFDNSTRTFTSSD 982

Query: 601  DSEIMEALQNSSVGQAADFKKTQKQCMPFLRFKKDEAAALGVQALDLKLPFGEMDVLREN 660
            D EI+EA+QNSSV    +FK TQK CMPF+RFKKDEA ALG++ALDL+LPFGEM+VL EN
Sbjct: 983  DGEILEAIQNSSVRPNDNFKMTQKLCMPFMRFKKDEAIALGIEALDLRLPFGEMEVLEEN 1042

Query: 661  LELIKRQIGLEEVEILCASDPDAVAKAGALASLLTQNPPSPGNPTAIFLT 708
            L+LIKRQIGLEEVE+L  +DPDA+AKAG+L  L+  NPPSPGNPTAIFL+
Sbjct: 1043 LDLIKRQIGLEEVEVLSITDPDALAKAGSLVRLIQLNPPSPGNPTAIFLS 1090

BLAST of Cp4.1LG00g04890 vs. TAIR10
Match: AT1G09620.1 (AT1G09620.1 ATP binding;leucine-tRNA ligases;aminoacyl-tRNA ligases;nucleotide binding;ATP binding;aminoacyl-tRNA ligases)

HSP 1 Score: 1095.1 bits (2831), Expect = 0.0e+00
Identity = 522/708 (73.73%), Postives = 612/708 (86.44%), Query Frame = 1

Query: 1    MLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRAKYGVKDEWVLPYDIVPIIDIPE 60
            MLTILT+KGTGIVTSVPSDAPDDYMA+ DL  KPAL+ KYGVK EW LP +I+PII+IPE
Sbjct: 381  MLTILTNKGTGIVTSVPSDAPDDYMALQDLIKKPALQDKYGVKTEW-LPTEIIPIINIPE 440

Query: 61   FGDRAAEKVCVDLKIKSQNEKDKLAEAKRLTYLKGFTDGTLIVGEFSGRKVQEAKPMIRS 120
            FGD+AAEKVC+DLKIKSQN+K+KLAEAKRLTYLKGFT+GT+++GEF GRKVQE KP+I++
Sbjct: 441  FGDKAAEKVCLDLKIKSQNDKEKLAEAKRLTYLKGFTEGTMLIGEFFGRKVQEIKPIIKT 500

Query: 121  KLIETGQAIPYSEPEKRVTSRSGDECIVALTDQWYITYGESEWKKLAEDCLANMNLFSDE 180
            KLIETG+AI YSEPEK V SRSGDEC+VALTDQWYITYGESEW+K+AE+CL+ MNL+SDE
Sbjct: 501  KLIETGEAIIYSEPEKPVMSRSGDECVVALTDQWYITYGESEWRKIAEECLSKMNLYSDE 560

Query: 181  TRHGFQHTLSWLNQWACSRSFGLGTRIPWDEQFLVESLSDSTIYMAYYTIAHFLHNGDLY 240
            TRHGF+HTLSWLNQWACSRSFGLGTRIPWDEQFLVESLSDS++YMAYYT+AH  H+GD+Y
Sbjct: 561  TRHGFEHTLSWLNQWACSRSFGLGTRIPWDEQFLVESLSDSSLYMAYYTVAHIFHDGDMY 620

Query: 241  ASDASAIKPEQMTDEVWDFIFCGAAEPKSTGISQSVLNKMKQEFEYWYPFDLRVSGKDLI 300
                S I+P+QM DEVW+++FC    PKS+ I  +VL++MKQEF+YWYP DLRVSGKDLI
Sbjct: 621  KGSKSLIRPQQMNDEVWEYLFCDGPYPKSSDIPSAVLSEMKQEFDYWYPLDLRVSGKDLI 680

Query: 301  QNHLTFCIYNHAAIMPRHHWPRGFRCNGHIMLNSEKMSKSTGNFRTLRQAIEEFSADATR 360
            QNHLTF IYNH A+M   +WPRG RCNGHIMLNSEKMSKSTGNFRTLRQ+IEEFSA  TR
Sbjct: 681  QNHLTFFIYNHTALMANRNWPRGIRCNGHIMLNSEKMSKSTGNFRTLRQSIEEFSATGTR 740

Query: 361  FSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDILQADSASFLRTGPPSTYADRVFE 420
            F LADAGDGVDDANF FETANAAILRLTKE+ WME++L  +S+  LRTGPPSTYAD+VFE
Sbjct: 741  FCLADAGDGVDDANFAFETANAAILRLTKELTWMEEVLDVESS--LRTGPPSTYADKVFE 800

Query: 421  NEINIAVRTTEKNYKDYMFREALKTGFYDLQAARDEYRFSCGAGGMNRDLVFRFMDVQTR 480
            N++NIA+R TE+ YKD +FREALK GFYDLQAARDEYR SCG GGM+ DL+ +FMDVQTR
Sbjct: 801  NDMNIALRLTERAYKDCLFREALKNGFYDLQAARDEYRLSCGTGGMHHDLLLKFMDVQTR 860

Query: 481  LITPICPHYAEYVWREMLKKEGFVINSGWPLADSPDLTLKIANKYLQDSIVLMRKLLQKQ 540
            LI PICPH+A+YVWR++L KEG V+ +GWP ++ PDL LK ANKYLQDSIVLMRKLLQKQ
Sbjct: 861  LIVPICPHFADYVWRKVLNKEGCVLTAGWPPSNEPDLVLKSANKYLQDSIVLMRKLLQKQ 920

Query: 541  LLGSKKGNKKGAPVTMVIEDKKLTGLVYVNEQFDGWKAECLRILRSKFDPTRRTFAPDSE 600
            L GSKKG KKGA VT V E  KL GLVYVNEQFDGW+A CLRIL+S+FD    +F PD+E
Sbjct: 921  LSGSKKGAKKGAQVTAVPEG-KLKGLVYVNEQFDGWRAHCLRILQSRFDQQTCSFPPDTE 980

Query: 601  IMEALQNSSVGQAADFKKTQKQCMPFLRFKKDEAAALGVQALDLKLPFGEMDVLRENLEL 660
            ++  L  + + +  + K  QK CMPFL+FKKDEA ++G QAL+L+LPFGE++VL+ N +L
Sbjct: 981  MLAELSATLLQEGKNLKAIQKVCMPFLKFKKDEAISIGTQALNLRLPFGEIEVLQSNKDL 1040

Query: 661  IKRQIGLEEVEILCASDPDAVAKAGALASLLTQNPPSPGNPTAIFLTS 709
            I+RQ+GLEEVEI  ASDPD V+ AG  ASLLTQNPPSPG+PTAIF+TS
Sbjct: 1041 IRRQLGLEEVEIYSASDPDDVSIAGPHASLLTQNPPSPGSPTAIFVTS 1084

BLAST of Cp4.1LG00g04890 vs. TAIR10
Match: AT5G16715.1 (AT5G16715.1 ATP binding;valine-tRNA ligases;aminoacyl-tRNA ligases;nucleotide binding;ATP binding;aminoacyl-tRNA ligases)

HSP 1 Score: 62.8 bits (151), Expect = 1.0e-09
Identity = 107/462 (23.16%), Postives = 171/462 (37.01%), Query Frame = 1

Query: 98  DGTL--IVGEFSGRKVQEAKPMIRSKLIETGQAIPYSEPEKRVT-SRSGDECIVALTD-Q 157
           D TL  + G F G    E +  + + L E G A+       RV  S+ G E I  L   Q
Sbjct: 365 DATLNDVAGLFCGLDRFEVREKLWADLEEIGLAVKKEPHTLRVPRSQRGGEVIEPLVSKQ 424

Query: 158 WYITYGESEWKKLAEDCLANMNLFSDETRHGFQHTLSWLNQWACSRSFGLGTRIP-W--- 217
           W++       K L       + +  +     + H L+ +  W  SR    G RIP W   
Sbjct: 425 WFVHMDPLAEKALLAVENKELTIIPERFEKIYNHWLTNIKDWCISRQLWWGHRIPVWYVV 484

Query: 218 ----DEQFLVESLSDSTIYMAYYTIAHFLHNGDLYASDASAIKPEQMTDEVWDFIFCGAA 277
               +E ++V   ++  +  A           + Y  D    +   + D  W   F  + 
Sbjct: 485 GKDCEEDYIVAKSAEEALEKAL----------EKYGKDVEIYQDPDVLD-TW---FSSSL 544

Query: 278 EPKSTGISQSVLNKMKQEFEYWYPFDLRVSGKDL----IQNHLTFCIYNHAAIMPRHHWP 337
            P ST     V  K   +F  +YP ++  +G D+    +   +   I     +   H + 
Sbjct: 545 WPFSTLGWPDVAAK---DFNNFYPTNMLETGHDILFFWVARMVMMGIEFTGTVPFSHVYL 604

Query: 338 RGFRCNGHIMLNSEKMSKSTGNFRTLRQAIEEFSADATRFSLADAGDGVDDANFVFE--T 397
            G   +        KMSKS GN       I++F  DA RF++A  G    D N   E  T
Sbjct: 605 HGLIRDSQ----GRKMSKSLGNVIDPLDTIKDFGTDALRFTIA-LGTAGQDLNLSTERLT 664

Query: 398 ANAAILR---------------LTKEIAWMEDI-LQADSASFLRTGP-PSTYADRVFENE 457
           AN A                  L+   AW   + L+ D    L + P P  +A       
Sbjct: 665 ANKAFTNKLWNAGKFVLHSLPSLSDTSAWENLLDLKLDKEETLLSLPLPECWAVSKLHIL 724

Query: 458 INIAVRTTEKNYKDYMFREALK---TGFYDLQAARDEYRFSCGAGGMNRD------LVFR 516
           I+    + EK +   + RE      + F D      + R   G+GG +        L++ 
Sbjct: 725 IDSVTASYEKLFFGDVGRETYDFFWSDFADWYIEASKSRL-YGSGGNSVSLASQAVLLYV 784

BLAST of Cp4.1LG00g04890 vs. NCBI nr
Match: gi|659072205|ref|XP_008463912.1| (PREDICTED: leucine--tRNA ligase, cytoplasmic [Cucumis melo])

HSP 1 Score: 1340.5 bits (3468), Expect = 0.0e+00
Identity = 645/708 (91.10%), Postives = 683/708 (96.47%), Query Frame = 1

Query: 1    MLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRAKYGVKDEWVLPYDIVPIIDIPE 60
            MLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRAKYGVKDEWVLPYDIVPIIDIPE
Sbjct: 383  MLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRAKYGVKDEWVLPYDIVPIIDIPE 442

Query: 61   FGDRAAEKVCVDLKIKSQNEKDKLAEAKRLTYLKGFTDGTLIVGEFSGRKVQEAKPMIRS 120
            FGDRAAEKVC+DLKIKSQNEKDKLAEAKRLTYL+GFTDGTL+VGEF+GRKVQEAKP+IRS
Sbjct: 443  FGDRAAEKVCLDLKIKSQNEKDKLAEAKRLTYLRGFTDGTLVVGEFAGRKVQEAKPLIRS 502

Query: 121  KLIETGQAIPYSEPEKRVTSRSGDECIVALTDQWYITYGESEWKKLAEDCLANMNLFSDE 180
            +LIETGQAIPYSEPEKRV SRSGDECIVALTDQWYITYGESEWKKL+E+CLA+M++FSDE
Sbjct: 503  QLIETGQAIPYSEPEKRVMSRSGDECIVALTDQWYITYGESEWKKLSEECLASMDMFSDE 562

Query: 181  TRHGFQHTLSWLNQWACSRSFGLGTRIPWDEQFLVESLSDSTIYMAYYTIAHFLHNGDLY 240
            T+HGF+HTL WLNQWACSRSFGLGTRIPWD+QFLVESLSDSTIYMAYYTIAH L NGDLY
Sbjct: 563  TKHGFEHTLGWLNQWACSRSFGLGTRIPWDKQFLVESLSDSTIYMAYYTIAHLLQNGDLY 622

Query: 241  ASDASAIKPEQMTDEVWDFIFCGAAEPKSTGISQSVLNKMKQEFEYWYPFDLRVSGKDLI 300
             S  SA+KPEQMTDEVWDF+FCGAAEPKSTGISQS+LNKMKQEFEYWYPFDLRVSGKDLI
Sbjct: 623  GSGDSAVKPEQMTDEVWDFVFCGAAEPKSTGISQSILNKMKQEFEYWYPFDLRVSGKDLI 682

Query: 301  QNHLTFCIYNHAAIMPRHHWPRGFRCNGHIMLNSEKMSKSTGNFRTLRQAIEEFSADATR 360
            QNHLTF IYNH AIMP+ HWPR FRCNGHIMLNSEKMSKSTGNFRTLR+AIEEFSADATR
Sbjct: 683  QNHLTFTIYNHTAIMPKRHWPRAFRCNGHIMLNSEKMSKSTGNFRTLREAIEEFSADATR 742

Query: 361  FSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDILQADSASFLRTGPPSTYADRVFE 420
            FSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDILQADS+SFLRTGPPSTYADRVFE
Sbjct: 743  FSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDILQADSSSFLRTGPPSTYADRVFE 802

Query: 421  NEINIAVRTTEKNYKDYMFREALKTGFYDLQAARDEYRFSCGAGGMNRDLVFRFMDVQTR 480
            NEINIAV+ TE+NYKDYMFREALKTGFYDLQAARDEYRFSCGAGGMNRDLVFRFMDVQTR
Sbjct: 803  NEINIAVKMTEQNYKDYMFREALKTGFYDLQAARDEYRFSCGAGGMNRDLVFRFMDVQTR 862

Query: 481  LITPICPHYAEYVWREMLKKEGFVINSGWPLADSPDLTLKIANKYLQDSIVLMRKLLQKQ 540
            LITPICPHYAE+VWR MLKK+GFV+N+GWP ADSPDLTLK ANKYLQDSIVLMRKLLQKQ
Sbjct: 863  LITPICPHYAEHVWRNMLKKDGFVVNAGWPSADSPDLTLKSANKYLQDSIVLMRKLLQKQ 922

Query: 541  LLGSKKGNKKGAPVTMVIEDKKLTGLVYVNEQFDGWKAECLRILRSKFDPTRRTFAPDSE 600
            LLGSKKGNKKG PVT VIEDKKLTGL+YVNEQFDGWKAECLRIL+SKFD T+RTFAPDSE
Sbjct: 923  LLGSKKGNKKGTPVTTVIEDKKLTGLIYVNEQFDGWKAECLRILQSKFDSTKRTFAPDSE 982

Query: 601  IMEALQNSSVGQAADFKKTQKQCMPFLRFKKDEAAALGVQALDLKLPFGEMDVLRENLEL 660
            IMEALQ SSVGQAADF++TQK CMPFLRFKKDEA +LGVQALDL+LPFGEMDVL ENLEL
Sbjct: 983  IMEALQKSSVGQAADFRQTQKLCMPFLRFKKDEAVSLGVQALDLRLPFGEMDVLNENLEL 1042

Query: 661  IKRQIGLEEVEILCASDPDAVAKAGALASLLTQNPPSPGNPTAIFLTS 709
            I+RQIGLEEV++L  SDP+A+AKAGALASLL QNPPSPGNPTAIFLTS
Sbjct: 1043 IRRQIGLEEVQVLRVSDPNALAKAGALASLLKQNPPSPGNPTAIFLTS 1090

BLAST of Cp4.1LG00g04890 vs. NCBI nr
Match: gi|778658888|ref|XP_011653458.1| (PREDICTED: leucine--tRNA ligase, cytoplasmic [Cucumis sativus])

HSP 1 Score: 1338.6 bits (3463), Expect = 0.0e+00
Identity = 646/708 (91.24%), Postives = 682/708 (96.33%), Query Frame = 1

Query: 1    MLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRAKYGVKDEWVLPYDIVPIIDIPE 60
            MLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRAKYGVKDEWVLPYDIVPIIDIPE
Sbjct: 383  MLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRAKYGVKDEWVLPYDIVPIIDIPE 442

Query: 61   FGDRAAEKVCVDLKIKSQNEKDKLAEAKRLTYLKGFTDGTLIVGEFSGRKVQEAKPMIRS 120
            FGDRAAEKVC+DLKIKSQNEKDKLAEAKRLTYL+GFTDGTLIVGEF+GRKVQEAKP+IRS
Sbjct: 443  FGDRAAEKVCLDLKIKSQNEKDKLAEAKRLTYLRGFTDGTLIVGEFAGRKVQEAKPLIRS 502

Query: 121  KLIETGQAIPYSEPEKRVTSRSGDECIVALTDQWYITYGESEWKKLAEDCLANMNLFSDE 180
            +LIETGQAIPYSEPEKRV SRSGDECIVALTDQWYI YGESEWKKL+E+CLA+M++FSDE
Sbjct: 503  QLIETGQAIPYSEPEKRVMSRSGDECIVALTDQWYIIYGESEWKKLSEECLASMDMFSDE 562

Query: 181  TRHGFQHTLSWLNQWACSRSFGLGTRIPWDEQFLVESLSDSTIYMAYYTIAHFLHNGDLY 240
            TRHGF+HTL WLNQWACSRSFGLGTRIPWD+QFLVESLSDSTIYMAYYTIAH L NGDLY
Sbjct: 563  TRHGFEHTLGWLNQWACSRSFGLGTRIPWDKQFLVESLSDSTIYMAYYTIAHLLQNGDLY 622

Query: 241  ASDASAIKPEQMTDEVWDFIFCGAAEPKSTGISQSVLNKMKQEFEYWYPFDLRVSGKDLI 300
             S  SA+KPEQMTDEVWDF+FCGAAEPKSTGISQS+LNKMKQEFEYWYPFDLRVSGKDLI
Sbjct: 623  GSGDSAVKPEQMTDEVWDFVFCGAAEPKSTGISQSILNKMKQEFEYWYPFDLRVSGKDLI 682

Query: 301  QNHLTFCIYNHAAIMPRHHWPRGFRCNGHIMLNSEKMSKSTGNFRTLRQAIEEFSADATR 360
            QNHLTF IYNH AIMP+ HWPR FRCNGHIMLNSEKMSKSTGNFRTLR+AIEEFSADATR
Sbjct: 683  QNHLTFTIYNHTAIMPKRHWPRAFRCNGHIMLNSEKMSKSTGNFRTLREAIEEFSADATR 742

Query: 361  FSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDILQADSASFLRTGPPSTYADRVFE 420
            FSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDILQADS+SFLRTGPPSTYADRVFE
Sbjct: 743  FSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDILQADSSSFLRTGPPSTYADRVFE 802

Query: 421  NEINIAVRTTEKNYKDYMFREALKTGFYDLQAARDEYRFSCGAGGMNRDLVFRFMDVQTR 480
            NEINIAV+ TE+NYKDYMFREALKTGFYDLQAARDEYRFSCGAGGMNRDLVFRFMDVQTR
Sbjct: 803  NEINIAVKMTEQNYKDYMFREALKTGFYDLQAARDEYRFSCGAGGMNRDLVFRFMDVQTR 862

Query: 481  LITPICPHYAEYVWREMLKKEGFVINSGWPLADSPDLTLKIANKYLQDSIVLMRKLLQKQ 540
            LITPICPHYAE+VWR MLKK GFV+N+GWP ADSPDLTLK ANKYLQDSIVLMRKLLQKQ
Sbjct: 863  LITPICPHYAEHVWRNMLKKNGFVVNAGWPSADSPDLTLKSANKYLQDSIVLMRKLLQKQ 922

Query: 541  LLGSKKGNKKGAPVTMVIEDKKLTGLVYVNEQFDGWKAECLRILRSKFDPTRRTFAPDSE 600
            LLGSKKGNKKGAPVT V+EDKKLTGL+YVNEQFDGWKAECLRIL+SKFD T+RTFAPDSE
Sbjct: 923  LLGSKKGNKKGAPVTTVVEDKKLTGLIYVNEQFDGWKAECLRILQSKFDSTKRTFAPDSE 982

Query: 601  IMEALQNSSVGQAADFKKTQKQCMPFLRFKKDEAAALGVQALDLKLPFGEMDVLRENLEL 660
            IMEALQ SSVGQAADF++TQK CMPFLRFKKDEA +LGVQAL+L+LPFGEMDVL ENLEL
Sbjct: 983  IMEALQKSSVGQAADFRQTQKLCMPFLRFKKDEAVSLGVQALNLRLPFGEMDVLNENLEL 1042

Query: 661  IKRQIGLEEVEILCASDPDAVAKAGALASLLTQNPPSPGNPTAIFLTS 709
            I+RQIGLEEV+IL  SDP+A+AKAGALASLL QNPPSPGNPTAIFLTS
Sbjct: 1043 IRRQIGLEEVQILRVSDPNALAKAGALASLLKQNPPSPGNPTAIFLTS 1090

BLAST of Cp4.1LG00g04890 vs. NCBI nr
Match: gi|802563075|ref|XP_012066702.1| (PREDICTED: leucine--tRNA ligase, cytoplasmic [Jatropha curcas])

HSP 1 Score: 1257.3 bits (3252), Expect = 0.0e+00
Identity = 600/708 (84.75%), Postives = 663/708 (93.64%), Query Frame = 1

Query: 1    MLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRAKYGVKDEWVLPYDIVPIIDIPE 60
            MLTILTDKGTGIVTSVPSDAPDDYMA+HDLK+KPA RAKYGVKDEWVLP++IVPII+IP+
Sbjct: 381  MLTILTDKGTGIVTSVPSDAPDDYMALHDLKAKPAFRAKYGVKDEWVLPFEIVPIINIPD 440

Query: 61   FGDRAAEKVCVDLKIKSQNEKDKLAEAKRLTYLKGFTDGTLIVGEFSGRKVQEAKPMIRS 120
            FGD+AAEKVC+DLKIKSQNEKDKLAEAKRLTYL+GFTDGT++VGEF+GRKVQEAKP+IR+
Sbjct: 441  FGDKAAEKVCLDLKIKSQNEKDKLAEAKRLTYLRGFTDGTMLVGEFAGRKVQEAKPLIRA 500

Query: 121  KLIETGQAIPYSEPEKRVTSRSGDECIVALTDQWYITYGESEWKKLAEDCLANMNLFSDE 180
            KLIETG+AI YSEPEKRV SRSGDEC+VALTDQWYITYGE EWKKLAE+C +NMNL+SDE
Sbjct: 501  KLIETGEAIMYSEPEKRVVSRSGDECVVALTDQWYITYGEEEWKKLAEECFSNMNLYSDE 560

Query: 181  TRHGFQHTLSWLNQWACSRSFGLGTRIPWDEQFLVESLSDSTIYMAYYTIAHFLHNGDLY 240
            TRHGF+HTLSWLNQWACSRSFGLGTRIPWD++FLVESLSDSTIYMAYYT+AH LHN D+Y
Sbjct: 561  TRHGFEHTLSWLNQWACSRSFGLGTRIPWDKEFLVESLSDSTIYMAYYTVAHLLHNDDMY 620

Query: 241  ASD-ASAIKPEQMTDEVWDFIFCGAAEPKSTGISQSVLNKMKQEFEYWYPFDLRVSGKDL 300
             ++   ++KPEQMTDEVWDFI CG   PKS+ I  S+L+KMKQEFEYWYPFDLRVSGKDL
Sbjct: 621  GTNKPHSVKPEQMTDEVWDFIICGGPHPKSSDIPSSILDKMKQEFEYWYPFDLRVSGKDL 680

Query: 301  IQNHLTFCIYNHAAIMPRHHWPRGFRCNGHIMLNSEKMSKSTGNFRTLRQAIEEFSADAT 360
            IQNHLTFCIYNH AIM +HHWPRGFRCNGH+MLNSEKMSKSTGNFRT+RQAIEEFSADAT
Sbjct: 681  IQNHLTFCIYNHTAIMAKHHWPRGFRCNGHMMLNSEKMSKSTGNFRTVRQAIEEFSADAT 740

Query: 361  RFSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDILQADSASFLRTGPPSTYADRVF 420
            RFSLADAGDGVDDANFVFETANAAILRLTKEIAWME++L A+S+  LR GPPSTYADRVF
Sbjct: 741  RFSLADAGDGVDDANFVFETANAAILRLTKEIAWMEEVLAAESS--LRIGPPSTYADRVF 800

Query: 421  ENEINIAVRTTEKNYKDYMFREALKTGFYDLQAARDEYRFSCGAGGMNRDLVFRFMDVQT 480
            ENEINIAVR TE+NY+ YMFREALK+GFYDLQAARDEYRFSCG+GGMNRDLV+RFMDVQT
Sbjct: 801  ENEINIAVRMTEQNYRGYMFREALKSGFYDLQAARDEYRFSCGSGGMNRDLVWRFMDVQT 860

Query: 481  RLITPICPHYAEYVWREMLKKEGFVINSGWPLADSPDLTLKIANKYLQDSIVLMRKLLQK 540
            RLITPICPHYAEYVWRE+LKK+GFV+ +GWP+A SPDLTLK ANKYLQDSIVLMRKLLQK
Sbjct: 861  RLITPICPHYAEYVWRELLKKDGFVVKAGWPMAGSPDLTLKAANKYLQDSIVLMRKLLQK 920

Query: 541  QLLGSKKGNKKGAPVTMVIEDKKLTGLVYVNEQFDGWKAECLRILRSKFDPTRRTFAPDS 600
            QLLGSKKGNKKGAPV  + ED KLTGL+YVNEQFDGWKAECLRIL+SKFD   RT APD 
Sbjct: 921  QLLGSKKGNKKGAPVATLTED-KLTGLIYVNEQFDGWKAECLRILQSKFDHKNRTVAPDG 980

Query: 601  EIMEALQNSSVGQAADFKKTQKQCMPFLRFKKDEAAALGVQALDLKLPFGEMDVLRENLE 660
            EIM+AL+NSSVGQA +FK+TQK CMPFLRFKKDEA A+G QALDLKLPFGE++VL+ENL+
Sbjct: 981  EIMDALKNSSVGQATNFKQTQKLCMPFLRFKKDEAIAIGSQALDLKLPFGEIEVLKENLD 1040

Query: 661  LIKRQIGLEEVEILCASDPDAVAKAGALASLLTQNPPSPGNPTAIFLT 708
            LIKRQIGLEEVEIL A+DPDA+AKAG+L SLL QNPPSPGNPTAIFLT
Sbjct: 1041 LIKRQIGLEEVEILSAADPDALAKAGSLVSLLNQNPPSPGNPTAIFLT 1085

BLAST of Cp4.1LG00g04890 vs. NCBI nr
Match: gi|645223569|ref|XP_008218693.1| (PREDICTED: leucine--tRNA ligase, cytoplasmic isoform X2 [Prunus mume])

HSP 1 Score: 1225.7 bits (3170), Expect = 0.0e+00
Identity = 583/708 (82.34%), Postives = 651/708 (91.95%), Query Frame = 1

Query: 1    MLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRAKYGVKDEWVLPYDIVPIIDIPE 60
            MLT+LTDKGTGIVTSVP+D+PDDYMA+HDLK+KPALR KYGVKDEWV+P++I+PII+IP 
Sbjct: 382  MLTVLTDKGTGIVTSVPADSPDDYMALHDLKAKPALRGKYGVKDEWVMPFEIIPIINIPG 441

Query: 61   FGDRAAEKVCVDLKIKSQNEKDKLAEAKRLTYLKGFTDGTLIVGEFSGRKVQEAKPMIRS 120
            FG++AAEKVC DLKIKSQNEK+KLAEAKRLTYLKGFT+GTLIVGEF+GRKVQ+ KP+IRS
Sbjct: 442  FGNKAAEKVCADLKIKSQNEKEKLAEAKRLTYLKGFTEGTLIVGEFNGRKVQDVKPLIRS 501

Query: 121  KLIETGQAIPYSEPEKRVTSRSGDECIVALTDQWYITYGESEWKKLAEDCLANMNLFSDE 180
            KLIE  +AI YSEPEKRV SRSGDEC+VALTDQWYITYGE EWKKLAE+CL++MNL+SDE
Sbjct: 502  KLIEANEAIIYSEPEKRVVSRSGDECVVALTDQWYITYGEPEWKKLAEECLSSMNLYSDE 561

Query: 181  TRHGFQHTLSWLNQWACSRSFGLGTRIPWDEQFLVESLSDSTIYMAYYTIAHFLHNGDLY 240
            TRHGF+HTL WLNQWACSRSFGLGTRIPWDE+FLVESLSDSTIYMAYYTIAHFLHNGD+Y
Sbjct: 562  TRHGFEHTLGWLNQWACSRSFGLGTRIPWDEEFLVESLSDSTIYMAYYTIAHFLHNGDMY 621

Query: 241  ASDASAIKPEQMTDEVWDFIFCGAAEPKSTGISQSVLNKMKQEFEYWYPFDLRVSGKDLI 300
             S  SAIKP QMTDEVW++IFC    P+S+ IS S+LNKMKQEFEYWYPFD+RVSGKDLI
Sbjct: 622  GSSKSAIKPGQMTDEVWEYIFCDGPYPESSDISSSILNKMKQEFEYWYPFDIRVSGKDLI 681

Query: 301  QNHLTFCIYNHAAIMPRHHWPRGFRCNGHIMLNSEKMSKSTGNFRTLRQAIEEFSADATR 360
            QNHLTFCIYNH AIMP+ HWPRGFRCNGHIMLNSEKMSKSTGNFRT+RQAIEEFSADATR
Sbjct: 682  QNHLTFCIYNHTAIMPKKHWPRGFRCNGHIMLNSEKMSKSTGNFRTIRQAIEEFSADATR 741

Query: 361  FSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDILQADSASFLRTGPPSTYADRVFE 420
            FSLADAGDGVDDANFVFETANAAILRLTKEIAWME++L ADS+  LR GPPSTYADRVF 
Sbjct: 742  FSLADAGDGVDDANFVFETANAAILRLTKEIAWMEEVLAADSS--LRIGPPSTYADRVFL 801

Query: 421  NEINIAVRTTEKNYKDYMFREALKTGFYDLQAARDEYRFSCGAGGMNRDLVFRFMDVQTR 480
            NEINIAV  TE+NY+DYMFR ALKTGFYDLQAARDEYRFSCG+GGMNR+LV RFMDVQTR
Sbjct: 802  NEINIAVNRTEQNYRDYMFRGALKTGFYDLQAARDEYRFSCGSGGMNRELVLRFMDVQTR 861

Query: 481  LITPICPHYAEYVWREMLKKEGFVINSGWPLADSPDLTLKIANKYLQDSIVLMRKLLQKQ 540
            LITPICPHYAEYVWRE+LKKEGFV+N+GWP+AD+PDLTL+ +NKYLQDSIVLMRKL +KQ
Sbjct: 862  LITPICPHYAEYVWRELLKKEGFVVNAGWPVADAPDLTLQSSNKYLQDSIVLMRKLYEKQ 921

Query: 541  LLGSKKGNKKGAPVTMVIEDKKL-TGLVYVNEQFDGWKAECLRILRSKFDPTRRTFAPDS 600
              GSKK NKKGAPVT V E+K+L  GL+YVNEQFD WKAECLRIL+S FD   RTFAPD+
Sbjct: 922  RSGSKKVNKKGAPVTTVTENKQLPIGLIYVNEQFDEWKAECLRILQSNFDRDTRTFAPDT 981

Query: 601  EIMEALQNSSVGQAADFKKTQKQCMPFLRFKKDEAAALGVQALDLKLPFGEMDVLRENLE 660
             IMEALQ SS+GQ  DF++TQK CMPF++ KKD+A ALG QALDLKLPFGE+DVLRENL+
Sbjct: 982  VIMEALQRSSIGQTKDFRQTQKLCMPFMKMKKDQAVALGAQALDLKLPFGEIDVLRENLD 1041

Query: 661  LIKRQIGLEEVEILCASDPDAVAKAGALASLLTQNPPSPGNPTAIFLT 708
            LIKRQIGLEEVE+L ASDPDA+AKAG+L  L+ QNPPSPG+PTAIFL+
Sbjct: 1042 LIKRQIGLEEVEVLSASDPDALAKAGSLVKLVEQNPPSPGSPTAIFLS 1087

BLAST of Cp4.1LG00g04890 vs. NCBI nr
Match: gi|645223567|ref|XP_008218692.1| (PREDICTED: leucine--tRNA ligase, cytoplasmic isoform X1 [Prunus mume])

HSP 1 Score: 1225.7 bits (3170), Expect = 0.0e+00
Identity = 583/708 (82.34%), Postives = 651/708 (91.95%), Query Frame = 1

Query: 1    MLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRAKYGVKDEWVLPYDIVPIIDIPE 60
            MLT+LTDKGTGIVTSVP+D+PDDYMA+HDLK+KPALR KYGVKDEWV+P++I+PII+IP 
Sbjct: 382  MLTVLTDKGTGIVTSVPADSPDDYMALHDLKAKPALRGKYGVKDEWVMPFEIIPIINIPG 441

Query: 61   FGDRAAEKVCVDLKIKSQNEKDKLAEAKRLTYLKGFTDGTLIVGEFSGRKVQEAKPMIRS 120
            FG++AAEKVC DLKIKSQNEK+KLAEAKRLTYLKGFT+GTLIVGEF+GRKVQ+ KP+IRS
Sbjct: 442  FGNKAAEKVCADLKIKSQNEKEKLAEAKRLTYLKGFTEGTLIVGEFNGRKVQDVKPLIRS 501

Query: 121  KLIETGQAIPYSEPEKRVTSRSGDECIVALTDQWYITYGESEWKKLAEDCLANMNLFSDE 180
            KLIE  +AI YSEPEKRV SRSGDEC+VALTDQWYITYGE EWKKLAE+CL++MNL+SDE
Sbjct: 502  KLIEANEAIIYSEPEKRVVSRSGDECVVALTDQWYITYGEPEWKKLAEECLSSMNLYSDE 561

Query: 181  TRHGFQHTLSWLNQWACSRSFGLGTRIPWDEQFLVESLSDSTIYMAYYTIAHFLHNGDLY 240
            TRHGF+HTL WLNQWACSRSFGLGTRIPWDE+FLVESLSDSTIYMAYYTIAHFLHNGD+Y
Sbjct: 562  TRHGFEHTLGWLNQWACSRSFGLGTRIPWDEEFLVESLSDSTIYMAYYTIAHFLHNGDMY 621

Query: 241  ASDASAIKPEQMTDEVWDFIFCGAAEPKSTGISQSVLNKMKQEFEYWYPFDLRVSGKDLI 300
             S  SAIKP QMTDEVW++IFC    P+S+ IS S+LNKMKQEFEYWYPFD+RVSGKDLI
Sbjct: 622  GSSKSAIKPGQMTDEVWEYIFCDGPYPESSDISSSILNKMKQEFEYWYPFDIRVSGKDLI 681

Query: 301  QNHLTFCIYNHAAIMPRHHWPRGFRCNGHIMLNSEKMSKSTGNFRTLRQAIEEFSADATR 360
            QNHLTFCIYNH AIMP+ HWPRGFRCNGHIMLNSEKMSKSTGNFRT+RQAIEEFSADATR
Sbjct: 682  QNHLTFCIYNHTAIMPKKHWPRGFRCNGHIMLNSEKMSKSTGNFRTIRQAIEEFSADATR 741

Query: 361  FSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDILQADSASFLRTGPPSTYADRVFE 420
            FSLADAGDGVDDANFVFETANAAILRLTKEIAWME++L ADS+  LR GPPSTYADRVF 
Sbjct: 742  FSLADAGDGVDDANFVFETANAAILRLTKEIAWMEEVLAADSS--LRIGPPSTYADRVFL 801

Query: 421  NEINIAVRTTEKNYKDYMFREALKTGFYDLQAARDEYRFSCGAGGMNRDLVFRFMDVQTR 480
            NEINIAV  TE+NY+DYMFR ALKTGFYDLQAARDEYRFSCG+GGMNR+LV RFMDVQTR
Sbjct: 802  NEINIAVNRTEQNYRDYMFRGALKTGFYDLQAARDEYRFSCGSGGMNRELVLRFMDVQTR 861

Query: 481  LITPICPHYAEYVWREMLKKEGFVINSGWPLADSPDLTLKIANKYLQDSIVLMRKLLQKQ 540
            LITPICPHYAEYVWRE+LKKEGFV+N+GWP+AD+PDLTL+ +NKYLQDSIVLMRKL +KQ
Sbjct: 862  LITPICPHYAEYVWRELLKKEGFVVNAGWPVADAPDLTLQSSNKYLQDSIVLMRKLYEKQ 921

Query: 541  LLGSKKGNKKGAPVTMVIEDKKL-TGLVYVNEQFDGWKAECLRILRSKFDPTRRTFAPDS 600
              GSKK NKKGAPVT V E+K+L  GL+YVNEQFD WKAECLRIL+S FD   RTFAPD+
Sbjct: 922  RSGSKKVNKKGAPVTTVTENKQLPIGLIYVNEQFDEWKAECLRILQSNFDRDTRTFAPDT 981

Query: 601  EIMEALQNSSVGQAADFKKTQKQCMPFLRFKKDEAAALGVQALDLKLPFGEMDVLRENLE 660
             IMEALQ SS+GQ  DF++TQK CMPF++ KKD+A ALG QALDLKLPFGE+DVLRENL+
Sbjct: 982  VIMEALQRSSIGQTKDFRQTQKLCMPFMKMKKDQAVALGAQALDLKLPFGEIDVLRENLD 1041

Query: 661  LIKRQIGLEEVEILCASDPDAVAKAGALASLLTQNPPSPGNPTAIFLT 708
            LIKRQIGLEEVE+L ASDPDA+AKAG+L  L+ QNPPSPG+PTAIFL+
Sbjct: 1042 LIKRQIGLEEVEVLSASDPDALAKAGSLVKLVEQNPPSPGSPTAIFLS 1087