BLAST of Carg12321 vs. NCBI nr
Match:
XP_022948270.1 (leucine--tRNA ligase, cytoplasmic-like [Cucurbita moschata])
HSP 1 Score: 2230.7 bits (5779), Expect = 0.0e+00
Identity = 1090/1090 (100.00%), Postives = 1090/1090 (100.00%), Query Frame = 0
Query: 19 MASECGKSFARRDSLREIESKVRVLWEENDVFRAEACETPPKEGEKFFGNFPFPYMNGFL 78
MASECGKSFARRDSLREIESKVRVLWEENDVFRAEACETPPKEGEKFFGNFPFPYMNGFL
Sbjct: 1 MASECGKSFARRDSLREIESKVRVLWEENDVFRAEACETPPKEGEKFFGNFPFPYMNGFL 60
Query: 79 HLGHAFSLSKLEFAAAYHRLRGANVLLPFGFHCTGMPIKASADKLAREIQQFGDPPVFPR 138
HLGHAFSLSKLEFAAAYHRLRGANVLLPFGFHCTGMPIKASADKLAREIQQFGDPPVFPR
Sbjct: 61 HLGHAFSLSKLEFAAAYHRLRGANVLLPFGFHCTGMPIKASADKLAREIQQFGDPPVFPR 120
Query: 139 QMEEQENLKAESEDANESNPTLPDKFKGKKSKAVSKSSGQMYQWEIMRSFGLSDSEISKF 198
QMEEQENLKAESEDANESNPTLPDKFKGKKSKAVSKSSGQMYQWEIMRSFGLSDSEISKF
Sbjct: 121 QMEEQENLKAESEDANESNPTLPDKFKGKKSKAVSKSSGQMYQWEIMRSFGLSDSEISKF 180
Query: 199 QNPYNWLTFFPPLAMEDLKAFGLGCDWRRSFITTDMNPFFDSFVQWQMRKLKSMGKIVKD 258
QNPYNWLTFFPPLAMEDLKAFGLGCDWRRSFITTDMNPFFDSFVQWQMRKLKSMGKIVKD
Sbjct: 181 QNPYNWLTFFPPLAMEDLKAFGLGCDWRRSFITTDMNPFFDSFVQWQMRKLKSMGKIVKD 240
Query: 259 VRYTIYSPLDGQPCADHDRASGEGVQPQDYTLIKMEVVPPFPPKMGVLEGRKVYLAAATL 318
VRYTIYSPLDGQPCADHDRASGEGVQPQDYTLIKMEVVPPFPPKMGVLEGRKVYLAAATL
Sbjct: 241 VRYTIYSPLDGQPCADHDRASGEGVQPQDYTLIKMEVVPPFPPKMGVLEGRKVYLAAATL 300
Query: 319 RPETMYGQTNAWVLPEGKYGAFEINDTDVFIITERAALNLAYQKFSRVPEKPTCLIQLTG 378
RPETMYGQTNAWVLPEGKYGAFEINDTDVFIITERAALNLAYQKFSRVPEKPTCLIQLTG
Sbjct: 301 RPETMYGQTNAWVLPEGKYGAFEINDTDVFIITERAALNLAYQKFSRVPEKPTCLIQLTG 360
Query: 379 NDLIGLPLKSPLAFNEIIYALPMLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRA 438
NDLIGLPLKSPLAFNEIIYALPMLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRA
Sbjct: 361 NDLIGLPLKSPLAFNEIIYALPMLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRA 420
Query: 439 KYGVKDEWVLPYDIVPIIDIPEFGDRAAEKVCVDLKIKSQNEKDKLAEAKRLTYLKGFTD 498
KYGVKDEWVLPYDIVPIIDIPEFGDRAAEKVCVDLKIKSQNEKDKLAEAKRLTYLKGFTD
Sbjct: 421 KYGVKDEWVLPYDIVPIIDIPEFGDRAAEKVCVDLKIKSQNEKDKLAEAKRLTYLKGFTD 480
Query: 499 GTLIVGEFSGRKVQEAKPMIRSKLIETGQAIPYSEPEKRVTSRSGDECIVALTDQWYITY 558
GTLIVGEFSGRKVQEAKPMIRSKLIETGQAIPYSEPEKRVTSRSGDECIVALTDQWYITY
Sbjct: 481 GTLIVGEFSGRKVQEAKPMIRSKLIETGQAIPYSEPEKRVTSRSGDECIVALTDQWYITY 540
Query: 559 GESEWKKLAEDCLANMNLFSDETRHGFQHTLSWLNQWACSRSFGLGTRIPWDEQFLVESL 618
GESEWKKLAEDCLANMNLFSDETRHGFQHTLSWLNQWACSRSFGLGTRIPWDEQFLVESL
Sbjct: 541 GESEWKKLAEDCLANMNLFSDETRHGFQHTLSWLNQWACSRSFGLGTRIPWDEQFLVESL 600
Query: 619 SDSTIYMAYYTIAHLLHNGDLYGSGASAIKPEQMTDEVWDFVFCGAAEPKSTGISRSILN 678
SDSTIYMAYYTIAHLLHNGDLYGSGASAIKPEQMTDEVWDFVFCGAAEPKSTGISRSILN
Sbjct: 601 SDSTIYMAYYTIAHLLHNGDLYGSGASAIKPEQMTDEVWDFVFCGAAEPKSTGISRSILN 660
Query: 679 KMKQEFEYWYPFDLRVSGKDLIQNHLTFCIYNHTAIMPEHHWPRGFRCNGHIMLNSEKMS 738
KMKQEFEYWYPFDLRVSGKDLIQNHLTFCIYNHTAIMPEHHWPRGFRCNGHIMLNSEKMS
Sbjct: 661 KMKQEFEYWYPFDLRVSGKDLIQNHLTFCIYNHTAIMPEHHWPRGFRCNGHIMLNSEKMS 720
Query: 739 KSTGNFRTLRQAIEEFSADATRFSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDIL 798
KSTGNFRTLRQAIEEFSADATRFSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDIL
Sbjct: 721 KSTGNFRTLRQAIEEFSADATRFSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDIL 780
Query: 799 QADSSSFLRTGPPSTYADRVFENEINIAVKTTEQNYKDYMFREALKSGFYDLQAARDEYR 858
QADSSSFLRTGPPSTYADRVFENEINIAVKTTEQNYKDYMFREALKSGFYDLQAARDEYR
Sbjct: 781 QADSSSFLRTGPPSTYADRVFENEINIAVKTTEQNYKDYMFREALKSGFYDLQAARDEYR 840
Query: 859 FSCGAGGMNRDLVFRFMDVQTRLITPICPHYAEYVWREMLKKEGFVVNAGWPSAGSPDLT 918
FSCGAGGMNRDLVFRFMDVQTRLITPICPHYAEYVWREMLKKEGFVVNAGWPSAGSPDLT
Sbjct: 841 FSCGAGGMNRDLVFRFMDVQTRLITPICPHYAEYVWREMLKKEGFVVNAGWPSAGSPDLT 900
Query: 919 LKSANKYLQDSIVLMRKLLQKQLLGSKKGNKKGAPVTSVIEDKKLTGLIYVNEQFDGWKA 978
LKSANKYLQDSIVLMRKLLQKQLLGSKKGNKKGAPVTSVIEDKKLTGLIYVNEQFDGWKA
Sbjct: 901 LKSANKYLQDSIVLMRKLLQKQLLGSKKGNKKGAPVTSVIEDKKLTGLIYVNEQFDGWKA 960
Query: 979 ECLRILRCKFDSTKRTFAPDSEIMEALQKSSVGQASDFRQTQKLCMPFLRFKKDEVAALG 1038
ECLRILRCKFDSTKRTFAPDSEIMEALQKSSVGQASDFRQTQKLCMPFLRFKKDEVAALG
Sbjct: 961 ECLRILRCKFDSTKRTFAPDSEIMEALQKSSVGQASDFRQTQKLCMPFLRFKKDEVAALG 1020
Query: 1039 VHALDLKLPFGEIEVLKENLELIKRQIGLEEVEVLCASDTDAIAKAGALASLLKQNPPSP 1098
VHALDLKLPFGEIEVLKENLELIKRQIGLEEVEVLCASDTDAIAKAGALASLLKQNPPSP
Sbjct: 1021 VHALDLKLPFGEIEVLKENLELIKRQIGLEEVEVLCASDTDAIAKAGALASLLKQNPPSP 1080
Query: 1099 GNPTAIFLTS 1109
GNPTAIFLTS
Sbjct: 1081 GNPTAIFLTS 1090
BLAST of Carg12321 vs. NCBI nr
Match:
XP_023523654.1 (leucine--tRNA ligase, cytoplasmic-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 2218.7 bits (5748), Expect = 0.0e+00
Identity = 1084/1090 (99.45%), Postives = 1086/1090 (99.63%), Query Frame = 0
Query: 19 MASECGKSFARRDSLREIESKVRVLWEENDVFRAEACETPPKEGEKFFGNFPFPYMNGFL 78
MASECGKSFARRDSLREIESKVRVLWEENDVFRAEACETPPKEGEKFFGNFPFPYMNGFL
Sbjct: 1 MASECGKSFARRDSLREIESKVRVLWEENDVFRAEACETPPKEGEKFFGNFPFPYMNGFL 60
Query: 79 HLGHAFSLSKLEFAAAYHRLRGANVLLPFGFHCTGMPIKASADKLAREIQQFGDPPVFPR 138
HLGHAFSLSKLEFAAAYHRLRGANVLLPFGFHCTGMPIKASADKLAREIQQFGDPPVFPR
Sbjct: 61 HLGHAFSLSKLEFAAAYHRLRGANVLLPFGFHCTGMPIKASADKLAREIQQFGDPPVFPR 120
Query: 139 QMEEQENLKAESEDANESNPTLPDKFKGKKSKAVSKSSGQMYQWEIMRSFGLSDSEISKF 198
QMEEQENLKAESEDANESNPTLPDKFKGKKSKAVSKSSGQMYQWEIMRSFGLSDSEISKF
Sbjct: 121 QMEEQENLKAESEDANESNPTLPDKFKGKKSKAVSKSSGQMYQWEIMRSFGLSDSEISKF 180
Query: 199 QNPYNWLTFFPPLAMEDLKAFGLGCDWRRSFITTDMNPFFDSFVQWQMRKLKSMGKIVKD 258
Q PYNWLTFFPPLAMEDLKAFGLGCDWRRSFITTDMNPFFDSFVQWQMRKLKSMGKIVKD
Sbjct: 181 QTPYNWLTFFPPLAMEDLKAFGLGCDWRRSFITTDMNPFFDSFVQWQMRKLKSMGKIVKD 240
Query: 259 VRYTIYSPLDGQPCADHDRASGEGVQPQDYTLIKMEVVPPFPPKMGVLEGRKVYLAAATL 318
VRYTIYSPLDGQPCADHDRASGEGVQPQDYTLIKMEVVPPFPPKMGVLEGRKVYLAAATL
Sbjct: 241 VRYTIYSPLDGQPCADHDRASGEGVQPQDYTLIKMEVVPPFPPKMGVLEGRKVYLAAATL 300
Query: 319 RPETMYGQTNAWVLPEGKYGAFEINDTDVFIITERAALNLAYQKFSRVPEKPTCLIQLTG 378
RPETMYGQTNAWVLPEGKYGAFEINDTDVFIITER+ALNLAYQKFSRVPEKPTCLIQLTG
Sbjct: 301 RPETMYGQTNAWVLPEGKYGAFEINDTDVFIITERSALNLAYQKFSRVPEKPTCLIQLTG 360
Query: 379 NDLIGLPLKSPLAFNEIIYALPMLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRA 438
NDLIGLPLKSPLAFNEIIYALPMLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRA
Sbjct: 361 NDLIGLPLKSPLAFNEIIYALPMLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRA 420
Query: 439 KYGVKDEWVLPYDIVPIIDIPEFGDRAAEKVCVDLKIKSQNEKDKLAEAKRLTYLKGFTD 498
KYGVKDEWVLPYDIVPIIDIPEFGDRAAEKVCVDLKIKSQNEKDKLAEAKRLTYLKGFTD
Sbjct: 421 KYGVKDEWVLPYDIVPIIDIPEFGDRAAEKVCVDLKIKSQNEKDKLAEAKRLTYLKGFTD 480
Query: 499 GTLIVGEFSGRKVQEAKPMIRSKLIETGQAIPYSEPEKRVTSRSGDECIVALTDQWYITY 558
GTLIVGEFSGRKVQEAKPMIRSKLIETGQAIPYSEPEKRVTSRSGDECIVALTDQWYITY
Sbjct: 481 GTLIVGEFSGRKVQEAKPMIRSKLIETGQAIPYSEPEKRVTSRSGDECIVALTDQWYITY 540
Query: 559 GESEWKKLAEDCLANMNLFSDETRHGFQHTLSWLNQWACSRSFGLGTRIPWDEQFLVESL 618
GESEWKKLAEDCLANMNLFSDETRHGFQHTLSWLNQWACSRSFGLGTRIPWDEQFLVESL
Sbjct: 541 GESEWKKLAEDCLANMNLFSDETRHGFQHTLSWLNQWACSRSFGLGTRIPWDEQFLVESL 600
Query: 619 SDSTIYMAYYTIAHLLHNGDLYGSGASAIKPEQMTDEVWDFVFCGAAEPKSTGISRSILN 678
SDSTIYMAYYTIAHLLHNGDLYGSG SAIKPEQMTDEVWDFVFCGAAEPKSTGISRSILN
Sbjct: 601 SDSTIYMAYYTIAHLLHNGDLYGSGTSAIKPEQMTDEVWDFVFCGAAEPKSTGISRSILN 660
Query: 679 KMKQEFEYWYPFDLRVSGKDLIQNHLTFCIYNHTAIMPEHHWPRGFRCNGHIMLNSEKMS 738
KMKQEFEYWYPFDLRVSGKDLIQNHLTFCIYNHTAIMPEHHWPRGFRCNGHIMLNSEKMS
Sbjct: 661 KMKQEFEYWYPFDLRVSGKDLIQNHLTFCIYNHTAIMPEHHWPRGFRCNGHIMLNSEKMS 720
Query: 739 KSTGNFRTLRQAIEEFSADATRFSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDIL 798
KSTGNFRTLRQAIEEFSADATRFSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDIL
Sbjct: 721 KSTGNFRTLRQAIEEFSADATRFSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDIL 780
Query: 799 QADSSSFLRTGPPSTYADRVFENEINIAVKTTEQNYKDYMFREALKSGFYDLQAARDEYR 858
QADSSSFLR GPPSTYADRVFENEINIAVKTTEQNYKDYMFREALKSGFYDLQAARDEYR
Sbjct: 781 QADSSSFLRKGPPSTYADRVFENEINIAVKTTEQNYKDYMFREALKSGFYDLQAARDEYR 840
Query: 859 FSCGAGGMNRDLVFRFMDVQTRLITPICPHYAEYVWREMLKKEGFVVNAGWPSAGSPDLT 918
FSCGAGGMNR+LVFRFMDVQTRLITPICPHYAEYVWREMLKKEGFVVNAGWPSA SPDLT
Sbjct: 841 FSCGAGGMNRNLVFRFMDVQTRLITPICPHYAEYVWREMLKKEGFVVNAGWPSADSPDLT 900
Query: 919 LKSANKYLQDSIVLMRKLLQKQLLGSKKGNKKGAPVTSVIEDKKLTGLIYVNEQFDGWKA 978
LKSANKYLQDSIVLMRKLLQKQLLGSKKGNKKGAPVTSVIEDKKLTGLIYVNEQFDGWKA
Sbjct: 901 LKSANKYLQDSIVLMRKLLQKQLLGSKKGNKKGAPVTSVIEDKKLTGLIYVNEQFDGWKA 960
Query: 979 ECLRILRCKFDSTKRTFAPDSEIMEALQKSSVGQASDFRQTQKLCMPFLRFKKDEVAALG 1038
ECLRILRCKFDSTKRTFAPDSEIMEALQKSSVGQASDFRQTQKLCMPFLRFKKDEVAALG
Sbjct: 961 ECLRILRCKFDSTKRTFAPDSEIMEALQKSSVGQASDFRQTQKLCMPFLRFKKDEVAALG 1020
Query: 1039 VHALDLKLPFGEIEVLKENLELIKRQIGLEEVEVLCASDTDAIAKAGALASLLKQNPPSP 1098
VHALDLKLPFGEIEVLKENLELIKRQIGLEEVEVLCASDTDAIAKAGALASLLKQNPPSP
Sbjct: 1021 VHALDLKLPFGEIEVLKENLELIKRQIGLEEVEVLCASDTDAIAKAGALASLLKQNPPSP 1080
Query: 1099 GNPTAIFLTS 1109
GNPTAIFLTS
Sbjct: 1081 GNPTAIFLTS 1090
BLAST of Carg12321 vs. NCBI nr
Match:
XP_022998939.1 (leucine--tRNA ligase, cytoplasmic-like [Cucurbita maxima])
HSP 1 Score: 2198.3 bits (5695), Expect = 0.0e+00
Identity = 1073/1090 (98.44%), Postives = 1083/1090 (99.36%), Query Frame = 0
Query: 19 MASECGKSFARRDSLREIESKVRVLWEENDVFRAEACETPPKEGEKFFGNFPFPYMNGFL 78
M SECGKSFARRDSLREIESKVRVLWEENDVFRAEACETPPKEGEKFFGNFPFPYMNGFL
Sbjct: 1 MTSECGKSFARRDSLREIESKVRVLWEENDVFRAEACETPPKEGEKFFGNFPFPYMNGFL 60
Query: 79 HLGHAFSLSKLEFAAAYHRLRGANVLLPFGFHCTGMPIKASADKLAREIQQFGDPPVFPR 138
HLGHAFSLSKLEFAAAYHRLRGANVLLPFGFHCTGMPIKASADKLAREI+QFGDPPVFP
Sbjct: 61 HLGHAFSLSKLEFAAAYHRLRGANVLLPFGFHCTGMPIKASADKLAREIKQFGDPPVFPS 120
Query: 139 QMEEQENLKAESEDANESNPTLPDKFKGKKSKAVSKSSGQMYQWEIMRSFGLSDSEISKF 198
Q+EE+ENLKAESEDANESNPTLPDKFKGKKSKAVSKSSGQMYQWEIMRSFGLSDSEISKF
Sbjct: 121 QIEEEENLKAESEDANESNPTLPDKFKGKKSKAVSKSSGQMYQWEIMRSFGLSDSEISKF 180
Query: 199 QNPYNWLTFFPPLAMEDLKAFGLGCDWRRSFITTDMNPFFDSFVQWQMRKLKSMGKIVKD 258
QNPYNWLTFFPPLAMEDLKAFGLGCDWRRSFITTDMNPFFDSFVQWQMRKLKSMGKIVKD
Sbjct: 181 QNPYNWLTFFPPLAMEDLKAFGLGCDWRRSFITTDMNPFFDSFVQWQMRKLKSMGKIVKD 240
Query: 259 VRYTIYSPLDGQPCADHDRASGEGVQPQDYTLIKMEVVPPFPPKMGVLEGRKVYLAAATL 318
VRYTIYSPLDGQPCADHDRASGEGVQPQDYTLIKMEVVPPFPPKM VLEGRKVYLAAATL
Sbjct: 241 VRYTIYSPLDGQPCADHDRASGEGVQPQDYTLIKMEVVPPFPPKMSVLEGRKVYLAAATL 300
Query: 319 RPETMYGQTNAWVLPEGKYGAFEINDTDVFIITERAALNLAYQKFSRVPEKPTCLIQLTG 378
RPETMYGQTNAWVLPEGKYGAFEINDTDV+IITERAALNLAYQKFSRVPEKPTCLIQLTG
Sbjct: 301 RPETMYGQTNAWVLPEGKYGAFEINDTDVYIITERAALNLAYQKFSRVPEKPTCLIQLTG 360
Query: 379 NDLIGLPLKSPLAFNEIIYALPMLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRA 438
NDLIGLPLKSPLAFNEIIYALPMLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRA
Sbjct: 361 NDLIGLPLKSPLAFNEIIYALPMLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRA 420
Query: 439 KYGVKDEWVLPYDIVPIIDIPEFGDRAAEKVCVDLKIKSQNEKDKLAEAKRLTYLKGFTD 498
KYGVKDEWVLPYDIVPII+IPEFGDRAAEKVCVDLKIKSQNEKDKLAEAKRLTYLKGFTD
Sbjct: 421 KYGVKDEWVLPYDIVPIINIPEFGDRAAEKVCVDLKIKSQNEKDKLAEAKRLTYLKGFTD 480
Query: 499 GTLIVGEFSGRKVQEAKPMIRSKLIETGQAIPYSEPEKRVTSRSGDECIVALTDQWYITY 558
GTLIVGEFSGRKVQEAKPMIRSKLIETGQAIPYSEPEKRV SRSGDECIVALTDQWYITY
Sbjct: 481 GTLIVGEFSGRKVQEAKPMIRSKLIETGQAIPYSEPEKRVISRSGDECIVALTDQWYITY 540
Query: 559 GESEWKKLAEDCLANMNLFSDETRHGFQHTLSWLNQWACSRSFGLGTRIPWDEQFLVESL 618
GESEWKKLAEDCLANMNLFSDETRHGFQHTLSWLNQWACSRSFGLGTRIPWDEQFLVESL
Sbjct: 541 GESEWKKLAEDCLANMNLFSDETRHGFQHTLSWLNQWACSRSFGLGTRIPWDEQFLVESL 600
Query: 619 SDSTIYMAYYTIAHLLHNGDLYGSGASAIKPEQMTDEVWDFVFCGAAEPKSTGISRSILN 678
SDSTIYMAYYTIAHLLH+GDLYGSGASAIKPEQMTDEVWDFVF GAAEPKSTGISRSILN
Sbjct: 601 SDSTIYMAYYTIAHLLHSGDLYGSGASAIKPEQMTDEVWDFVFFGAAEPKSTGISRSILN 660
Query: 679 KMKQEFEYWYPFDLRVSGKDLIQNHLTFCIYNHTAIMPEHHWPRGFRCNGHIMLNSEKMS 738
KMKQEFEYWYPFDLRVSGKDLIQNHLTFCIYNHTAIMPEHHWPRGFRCNGHIMLNSEKMS
Sbjct: 661 KMKQEFEYWYPFDLRVSGKDLIQNHLTFCIYNHTAIMPEHHWPRGFRCNGHIMLNSEKMS 720
Query: 739 KSTGNFRTLRQAIEEFSADATRFSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDIL 798
KSTGNFRTLRQAIEEFSADATRFSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDIL
Sbjct: 721 KSTGNFRTLRQAIEEFSADATRFSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDIL 780
Query: 799 QADSSSFLRTGPPSTYADRVFENEINIAVKTTEQNYKDYMFREALKSGFYDLQAARDEYR 858
QADSSSFLRTGPPSTYADRVFENE+NIAVKTTEQNYK+YMFREALKSGFYDLQAARDEYR
Sbjct: 781 QADSSSFLRTGPPSTYADRVFENEMNIAVKTTEQNYKNYMFREALKSGFYDLQAARDEYR 840
Query: 859 FSCGAGGMNRDLVFRFMDVQTRLITPICPHYAEYVWREMLKKEGFVVNAGWPSAGSPDLT 918
FSCGAGGMNRDLVFRFMDVQTRLITPICPHYAEYVWREMLKKEGFVVNAGWPSAG PDLT
Sbjct: 841 FSCGAGGMNRDLVFRFMDVQTRLITPICPHYAEYVWREMLKKEGFVVNAGWPSAGYPDLT 900
Query: 919 LKSANKYLQDSIVLMRKLLQKQLLGSKKGNKKGAPVTSVIEDKKLTGLIYVNEQFDGWKA 978
LKSANKYLQDSIVLMRKLLQKQLLGSKKGNKKGAPVT VIEDKKLTGLIYVNEQFDGWKA
Sbjct: 901 LKSANKYLQDSIVLMRKLLQKQLLGSKKGNKKGAPVTYVIEDKKLTGLIYVNEQFDGWKA 960
Query: 979 ECLRILRCKFDSTKRTFAPDSEIMEALQKSSVGQASDFRQTQKLCMPFLRFKKDEVAALG 1038
ECLRIL+CKFDSTKRTFAPDSEIMEALQKSSVGQASDFRQTQKLCMPFLRFKKDEVAALG
Sbjct: 961 ECLRILQCKFDSTKRTFAPDSEIMEALQKSSVGQASDFRQTQKLCMPFLRFKKDEVAALG 1020
Query: 1039 VHALDLKLPFGEIEVLKENLELIKRQIGLEEVEVLCASDTDAIAKAGALASLLKQNPPSP 1098
VHALDLKLPFGEIEVLKENLELIKRQIGLEEVEVLCASDTDAIAK+GALASLLKQNPPSP
Sbjct: 1021 VHALDLKLPFGEIEVLKENLELIKRQIGLEEVEVLCASDTDAIAKSGALASLLKQNPPSP 1080
Query: 1099 GNPTAIFLTS 1109
GNPTAIFLTS
Sbjct: 1081 GNPTAIFLTS 1090
BLAST of Carg12321 vs. NCBI nr
Match:
XP_023521250.1 (leucine--tRNA ligase, cytoplasmic-like [Cucurbita pepo subsp. pepo] >XP_023521251.1 leucine--tRNA ligase, cytoplasmic-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 2159.4 bits (5594), Expect = 0.0e+00
Identity = 1047/1090 (96.06%), Postives = 1069/1090 (98.07%), Query Frame = 0
Query: 19 MASECGKSFARRDSLREIESKVRVLWEENDVFRAEACETPPKEGEKFFGNFPFPYMNGFL 78
MASE GKSFARRDSLR+IE+KVRVLWEENDVFRAEACETPPK EKFFGNFPFPYMNGFL
Sbjct: 1 MASESGKSFARRDSLRDIEAKVRVLWEENDVFRAEACETPPKADEKFFGNFPFPYMNGFL 60
Query: 79 HLGHAFSLSKLEFAAAYHRLRGANVLLPFGFHCTGMPIKASADKLAREIQQFGDPPVFPR 138
HLGHAFS+SKLEFAAAYHRLRGANVLLPFGFHCTGMPIKASADKLAREIQQFGDPP FPR
Sbjct: 61 HLGHAFSVSKLEFAAAYHRLRGANVLLPFGFHCTGMPIKASADKLAREIQQFGDPPAFPR 120
Query: 139 QMEEQENLKAESEDANESNPTLPDKFKGKKSKAVSKSSGQMYQWEIMRSFGLSDSEISKF 198
QMEEQENLKAESEDANESNPTLPDKFKGKKSKAVSKSSGQMYQWEIMRSFGLSDSEISKF
Sbjct: 121 QMEEQENLKAESEDANESNPTLPDKFKGKKSKAVSKSSGQMYQWEIMRSFGLSDSEISKF 180
Query: 199 QNPYNWLTFFPPLAMEDLKAFGLGCDWRRSFITTDMNPFFDSFVQWQMRKLKSMGKIVKD 258
QNPYNWLTFFPPLAMEDLKAFGLGCDWRRSFITTDMNPFFDSFVQWQMRKLKSMGKIVKD
Sbjct: 181 QNPYNWLTFFPPLAMEDLKAFGLGCDWRRSFITTDMNPFFDSFVQWQMRKLKSMGKIVKD 240
Query: 259 VRYTIYSPLDGQPCADHDRASGEGVQPQDYTLIKMEVVPPFPPKMGVLEGRKVYLAAATL 318
VRYTIYSPLDGQPCADHDRASGEGVQPQDYTLIKMEVVPPFPPKMGVLEGRKVYLAAATL
Sbjct: 241 VRYTIYSPLDGQPCADHDRASGEGVQPQDYTLIKMEVVPPFPPKMGVLEGRKVYLAAATL 300
Query: 319 RPETMYGQTNAWVLPEGKYGAFEINDTDVFIITERAALNLAYQKFSRVPEKPTCLIQLTG 378
RPETMYGQTNAWVLPEGKYGAFEINDTDVFIITERAALNLAYQKFSRVPEKPTCLIQLTG
Sbjct: 301 RPETMYGQTNAWVLPEGKYGAFEINDTDVFIITERAALNLAYQKFSRVPEKPTCLIQLTG 360
Query: 379 NDLIGLPLKSPLAFNEIIYALPMLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRA 438
NDLIGLPLKSPLAFNEIIYALPMLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRA
Sbjct: 361 NDLIGLPLKSPLAFNEIIYALPMLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRA 420
Query: 439 KYGVKDEWVLPYDIVPIIDIPEFGDRAAEKVCVDLKIKSQNEKDKLAEAKRLTYLKGFTD 498
KYGVKDEWVLPYDIVPIIDIPEFGDRAAEKVCVDLKIKSQNEKDKLAEAKRLTYLKGFTD
Sbjct: 421 KYGVKDEWVLPYDIVPIIDIPEFGDRAAEKVCVDLKIKSQNEKDKLAEAKRLTYLKGFTD 480
Query: 499 GTLIVGEFSGRKVQEAKPMIRSKLIETGQAIPYSEPEKRVTSRSGDECIVALTDQWYITY 558
GTLIVGEFSGRKVQEAKPMIRSKLIETGQAIPYSEPEKRVTSRSGDECIVALTDQWYITY
Sbjct: 481 GTLIVGEFSGRKVQEAKPMIRSKLIETGQAIPYSEPEKRVTSRSGDECIVALTDQWYITY 540
Query: 559 GESEWKKLAEDCLANMNLFSDETRHGFQHTLSWLNQWACSRSFGLGTRIPWDEQFLVESL 618
GESEWKKLAEDCLANMNLFSDETRHGFQHTLSWLNQWACSRSFGLGTRIPWDEQFLVESL
Sbjct: 541 GESEWKKLAEDCLANMNLFSDETRHGFQHTLSWLNQWACSRSFGLGTRIPWDEQFLVESL 600
Query: 619 SDSTIYMAYYTIAHLLHNGDLYGSGASAIKPEQMTDEVWDFVFCGAAEPKSTGISRSILN 678
SDSTIYMAYYTIAH LHNGDLY S ASAIKPEQMTDEVWDF+FCGAAEPKSTGIS+S+LN
Sbjct: 601 SDSTIYMAYYTIAHFLHNGDLYASDASAIKPEQMTDEVWDFIFCGAAEPKSTGISQSVLN 660
Query: 679 KMKQEFEYWYPFDLRVSGKDLIQNHLTFCIYNHTAIMPEHHWPRGFRCNGHIMLNSEKMS 738
KMKQEFEYWYPFDLRVSGKDLIQNHLTFCIYNH AIMP HHWPRGFRCNGHIMLNSEKMS
Sbjct: 661 KMKQEFEYWYPFDLRVSGKDLIQNHLTFCIYNHAAIMPRHHWPRGFRCNGHIMLNSEKMS 720
Query: 739 KSTGNFRTLRQAIEEFSADATRFSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDIL 798
KSTGNFRTLRQAIEEFSADATRFSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDIL
Sbjct: 721 KSTGNFRTLRQAIEEFSADATRFSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDIL 780
Query: 799 QADSSSFLRTGPPSTYADRVFENEINIAVKTTEQNYKDYMFREALKSGFYDLQAARDEYR 858
QADS+SFLRTGPPSTYADRVFENEINIAV+TTE+NYKDYMFREALK+GFYDLQAARDEYR
Sbjct: 781 QADSASFLRTGPPSTYADRVFENEINIAVRTTEKNYKDYMFREALKTGFYDLQAARDEYR 840
Query: 859 FSCGAGGMNRDLVFRFMDVQTRLITPICPHYAEYVWREMLKKEGFVVNAGWPSAGSPDLT 918
FSCGAGGMNRDLVFRFMDVQTRLITPICPHYAEYVWREMLKKEGFV+N+GWP A SPDLT
Sbjct: 841 FSCGAGGMNRDLVFRFMDVQTRLITPICPHYAEYVWREMLKKEGFVINSGWPLADSPDLT 900
Query: 919 LKSANKYLQDSIVLMRKLLQKQLLGSKKGNKKGAPVTSVIEDKKLTGLIYVNEQFDGWKA 978
LK ANKYLQDSIVLMRKLLQKQLLGSKKGNKKGAPVT VIEDKKLTGL+YVNEQFDGWKA
Sbjct: 901 LKIANKYLQDSIVLMRKLLQKQLLGSKKGNKKGAPVTMVIEDKKLTGLVYVNEQFDGWKA 960
Query: 979 ECLRILRCKFDSTKRTFAPDSEIMEALQKSSVGQASDFRQTQKLCMPFLRFKKDEVAALG 1038
ECLRILR KFD T+RTFAPDSEIMEALQ SSVGQA+DF++TQK CMPFLRFKKDE AALG
Sbjct: 961 ECLRILRSKFDPTRRTFAPDSEIMEALQNSSVGQAADFKKTQKQCMPFLRFKKDEAAALG 1020
Query: 1039 VHALDLKLPFGEIEVLKENLELIKRQIGLEEVEVLCASDTDAIAKAGALASLLKQNPPSP 1098
V ALDLKLPFGE++VL+ENLELIKRQIGLEEVE+LCASD DA+AKAGALASLL QNPPSP
Sbjct: 1021 VQALDLKLPFGEMDVLRENLELIKRQIGLEEVEILCASDPDAVAKAGALASLLTQNPPSP 1080
Query: 1099 GNPTAIFLTS 1109
GNPTAIFLTS
Sbjct: 1081 GNPTAIFLTS 1090
BLAST of Carg12321 vs. NCBI nr
Match:
XP_022970400.1 (leucine--tRNA ligase, cytoplasmic-like [Cucurbita maxima] >XP_022970401.1 leucine--tRNA ligase, cytoplasmic-like [Cucurbita maxima] >XP_022970402.1 leucine--tRNA ligase, cytoplasmic-like [Cucurbita maxima] >XP_022970403.1 leucine--tRNA ligase, cytoplasmic-like [Cucurbita maxima] >XP_022970404.1 leucine--tRNA ligase, cytoplasmic-like [Cucurbita maxima] >XP_022970406.1 leucine--tRNA ligase, cytoplasmic-like [Cucurbita maxima] >XP_022970407.1 leucine--tRNA ligase, cytoplasmic-like [Cucurbita maxima])
HSP 1 Score: 2123.6 bits (5501), Expect = 0.0e+00
Identity = 1026/1090 (94.13%), Postives = 1063/1090 (97.52%), Query Frame = 0
Query: 19 MASECGKSFARRDSLREIESKVRVLWEENDVFRAEACETPPKEGEKFFGNFPFPYMNGFL 78
MASE GKSFARRDSLR+IE+KVRVLWEENDVFRAEACETPPK GEKFFGNFPFPYMNGFL
Sbjct: 1 MASESGKSFARRDSLRDIEAKVRVLWEENDVFRAEACETPPKVGEKFFGNFPFPYMNGFL 60
Query: 79 HLGHAFSLSKLEFAAAYHRLRGANVLLPFGFHCTGMPIKASADKLAREIQQFGDPPVFPR 138
HLGHAFS+SKLEFAAAYHRLRGANVLLPFGFHCTGMPIKASADKLAREIQQFGDPPVFP
Sbjct: 61 HLGHAFSVSKLEFAAAYHRLRGANVLLPFGFHCTGMPIKASADKLAREIQQFGDPPVFPS 120
Query: 139 QMEEQENLKAESEDANESNPTLPDKFKGKKSKAVSKSSGQMYQWEIMRSFGLSDSEISKF 198
Q EEQENLKAE+ED NESNPTLPDKFKGKKSKA SKSSGQMYQWEIMRSFGLSDSEISKF
Sbjct: 121 QTEEQENLKAEAEDTNESNPTLPDKFKGKKSKAASKSSGQMYQWEIMRSFGLSDSEISKF 180
Query: 199 QNPYNWLTFFPPLAMEDLKAFGLGCDWRRSFITTDMNPFFDSFVQWQMRKLKSMGKIVKD 258
QNPYNWLTFFP +AMEDLKAFGLGCDWRRSFITTDMNPFF+SFVQWQMRKLKSMGKIVKD
Sbjct: 181 QNPYNWLTFFPTVAMEDLKAFGLGCDWRRSFITTDMNPFFNSFVQWQMRKLKSMGKIVKD 240
Query: 259 VRYTIYSPLDGQPCADHDRASGEGVQPQDYTLIKMEVVPPFPPKMGVLEGRKVYLAAATL 318
VRY IYS LDGQPCADHDRASGEGV PQDYTLIKMEVV PFPPK+GVLEGRKV+LAAATL
Sbjct: 241 VRYAIYSTLDGQPCADHDRASGEGVLPQDYTLIKMEVVAPFPPKLGVLEGRKVFLAAATL 300
Query: 319 RPETMYGQTNAWVLPEGKYGAFEINDTDVFIITERAALNLAYQKFSRVPEKPTCLIQLTG 378
RPETMYGQTNAWVLPEGKYGAFEINDTDVFIITERAALNLAYQKFSR+PEKPTCLIQLTG
Sbjct: 301 RPETMYGQTNAWVLPEGKYGAFEINDTDVFIITERAALNLAYQKFSRIPEKPTCLIQLTG 360
Query: 379 NDLIGLPLKSPLAFNEIIYALPMLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRA 438
NDLIGLPLKSPLAFNEIIYALPMLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRA
Sbjct: 361 NDLIGLPLKSPLAFNEIIYALPMLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRA 420
Query: 439 KYGVKDEWVLPYDIVPIIDIPEFGDRAAEKVCVDLKIKSQNEKDKLAEAKRLTYLKGFTD 498
KYGVKDEWVLPYDIVPIIDIPEFGDRAAEKVC+DLKIKSQNEKDKLAEAKRLTYLKGFTD
Sbjct: 421 KYGVKDEWVLPYDIVPIIDIPEFGDRAAEKVCLDLKIKSQNEKDKLAEAKRLTYLKGFTD 480
Query: 499 GTLIVGEFSGRKVQEAKPMIRSKLIETGQAIPYSEPEKRVTSRSGDECIVALTDQWYITY 558
GTLIVGEF+GRKVQEAKP+IRSKLIETG+AIPYSEPEKRV SRSGDECIVALTDQWYITY
Sbjct: 481 GTLIVGEFAGRKVQEAKPLIRSKLIETGEAIPYSEPEKRVISRSGDECIVALTDQWYITY 540
Query: 559 GESEWKKLAEDCLANMNLFSDETRHGFQHTLSWLNQWACSRSFGLGTRIPWDEQFLVESL 618
GESEWKKLAE+CLANM+LFSDETRHGF+HTLSWLNQWACSRSFGLGTRIPWDEQFLVESL
Sbjct: 541 GESEWKKLAEECLANMDLFSDETRHGFEHTLSWLNQWACSRSFGLGTRIPWDEQFLVESL 600
Query: 619 SDSTIYMAYYTIAHLLHNGDLYGSGASAIKPEQMTDEVWDFVFCGAAEPKSTGISRSILN 678
SDSTIYMAYYTIAH LHNGDLY S ASAIKPEQMTDEVWDF+FCGAAEPKSTGIS+S+LN
Sbjct: 601 SDSTIYMAYYTIAHFLHNGDLYASDASAIKPEQMTDEVWDFIFCGAAEPKSTGISQSVLN 660
Query: 679 KMKQEFEYWYPFDLRVSGKDLIQNHLTFCIYNHTAIMPEHHWPRGFRCNGHIMLNSEKMS 738
KMKQEFEYWYPFDLRVSGKDLIQNHLTFCIYNHTAIMP HHWPRGFRCNGHIMLNSEKMS
Sbjct: 661 KMKQEFEYWYPFDLRVSGKDLIQNHLTFCIYNHTAIMPRHHWPRGFRCNGHIMLNSEKMS 720
Query: 739 KSTGNFRTLRQAIEEFSADATRFSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDIL 798
KSTGNFRTLRQAIEEFSADATRFSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDIL
Sbjct: 721 KSTGNFRTLRQAIEEFSADATRFSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDIL 780
Query: 799 QADSSSFLRTGPPSTYADRVFENEINIAVKTTEQNYKDYMFREALKSGFYDLQAARDEYR 858
QADS+SFLR GPPSTYADRVFENEINIAV+TTE+NYK+YMFREALK+GFYDLQAARDEYR
Sbjct: 781 QADSASFLRIGPPSTYADRVFENEINIAVRTTEKNYKEYMFREALKTGFYDLQAARDEYR 840
Query: 859 FSCGAGGMNRDLVFRFMDVQTRLITPICPHYAEYVWREMLKKEGFVVNAGWPSAGSPDLT 918
FSCGAGGMNRDLVFRFMDVQTRLITPICPHYAEYVWREMLKKEGFV+N+GWP A SPDLT
Sbjct: 841 FSCGAGGMNRDLVFRFMDVQTRLITPICPHYAEYVWREMLKKEGFVINSGWPLADSPDLT 900
Query: 919 LKSANKYLQDSIVLMRKLLQKQLLGSKKGNKKGAPVTSVIEDKKLTGLIYVNEQFDGWKA 978
LK+ANKYLQDSIVLMRKLLQKQLLGSKKGNKKGAPVT VIEDKKLTGL+YVNEQFDGWKA
Sbjct: 901 LKTANKYLQDSIVLMRKLLQKQLLGSKKGNKKGAPVTMVIEDKKLTGLVYVNEQFDGWKA 960
Query: 979 ECLRILRCKFDSTKRTFAPDSEIMEALQKSSVGQASDFRQTQKLCMPFLRFKKDEVAALG 1038
ECLRILR KFD+T+RTFAPDSEIMEALQ SSVGQA+DF++TQK CMPFLRFKKDE AALG
Sbjct: 961 ECLRILRSKFDTTRRTFAPDSEIMEALQNSSVGQAADFKKTQKQCMPFLRFKKDEAAALG 1020
Query: 1039 VHALDLKLPFGEIEVLKENLELIKRQIGLEEVEVLCASDTDAIAKAGALASLLKQNPPSP 1098
V ALDLKLPFGEI+VL+ENLELIKRQIGLEEVE+LCASD DA+AKAGALASLL QNPPSP
Sbjct: 1021 VQALDLKLPFGEIDVLRENLELIKRQIGLEEVEILCASDPDALAKAGALASLLTQNPPSP 1080
Query: 1099 GNPTAIFLTS 1109
GNPTAIFLTS
Sbjct: 1081 GNPTAIFLTS 1090
BLAST of Carg12321 vs. TAIR10
Match:
AT1G09620.1 (ATP binding;leucine-tRNA ligases;aminoacyl-tRNA ligases;nucleotide binding;ATP binding;aminoacyl-tRNA ligases)
HSP 1 Score: 1652.9 bits (4279), Expect = 0.0e+00
Identity = 782/1090 (71.74%), Postives = 932/1090 (85.50%), Query Frame = 0
Query: 19 MASECGKSFARRDSLREIESKVRVLWEENDVFRAEACETPPKEGEKFFGNFPFPYMNGFL 78
MASE KS+ARRD L EIE+ VR WE+ DVFRAE+CE PK GEKFF FPFPYMNG+L
Sbjct: 1 MASE-SKSYARRDRLLEIEATVRKWWEDEDVFRAESCENLPKPGEKFFSTFPFPYMNGYL 60
Query: 79 HLGHAFSLSKLEFAAAYHRLRGANVLLPFGFHCTGMPIKASADKLAREIQQFGDPPVFPR 138
H+GHAFSLSK++FA+AYHRLRGANVLLPFGFHCTGMPIKASADKL REI+QFG+PPVF
Sbjct: 61 HIGHAFSLSKVDFASAYHRLRGANVLLPFGFHCTGMPIKASADKLRREIEQFGNPPVFTA 120
Query: 139 QMEEQENLKAESEDANESNPTLPDKFKGKKSKAVSKSSGQMYQWEIMRSFGLSDSEISKF 198
+ + + + + E ++ +P +FKGKKSK +K+ GQ+YQWEIMRSFGL+DSEI+ F
Sbjct: 121 E-DTTKVPEVQEESSDTIALPIPGQFKGKKSKVAAKAGGQVYQWEIMRSFGLTDSEIANF 180
Query: 199 QNPYNWLTFFPPLAMEDLKAFGLGCDWRRSFITTDMNPFFDSFVQWQMRKLKSMGKIVKD 258
+ P WL +FPPLA+EDL+A+GLGCDWRRSF+TTD+NPFFD+FV+WQMRKLKSMGKIVKD
Sbjct: 181 REPSEWLYYFPPLAVEDLRAYGLGCDWRRSFVTTDVNPFFDAFVRWQMRKLKSMGKIVKD 240
Query: 259 VRYTIYSPLDGQPCADHDRASGEGVQPQDYTLIKMEVVPPFPPKMGVLEGRKVYLAAATL 318
RYTI+SPLDGQPCADHDRA+GEGVQPQ+YTLIKMEVV PFP K+G LEG++V+LAAATL
Sbjct: 241 RRYTIFSPLDGQPCADHDRATGEGVQPQEYTLIKMEVVKPFPLKLGPLEGKRVFLAAATL 300
Query: 319 RPETMYGQTNAWVLPEGKYGAFEINDTDVFIITERAALNLAYQKFSRVPEKPTCLIQLTG 378
RPETMYGQTNAWVLP+GKYGA+EI++T+VFI+TERAALNLAYQ FS+ P++P+CL++LTG
Sbjct: 301 RPETMYGQTNAWVLPDGKYGAYEISETEVFILTERAALNLAYQNFSKNPQEPSCLVELTG 360
Query: 379 NDLIGLPLKSPLAFNEIIYALPMLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRA 438
DLIGLPL+SPL+ NEIIYALPMLTILT+KGTGIVTSVPSDAPDDYMA+ DL KPAL+
Sbjct: 361 YDLIGLPLRSPLSVNEIIYALPMLTILTNKGTGIVTSVPSDAPDDYMALQDLIKKPALQD 420
Query: 439 KYGVKDEWVLPYDIVPIIDIPEFGDRAAEKVCVDLKIKSQNEKDKLAEAKRLTYLKGFTD 498
KYGVK EW LP +I+PII+IPEFGD+AAEKVC+DLKIKSQN+K+KLAEAKRLTYLKGFT+
Sbjct: 421 KYGVKTEW-LPTEIIPIINIPEFGDKAAEKVCLDLKIKSQNDKEKLAEAKRLTYLKGFTE 480
Query: 499 GTLIVGEFSGRKVQEAKPMIRSKLIETGQAIPYSEPEKRVTSRSGDECIVALTDQWYITY 558
GT+++GEF GRKVQE KP+I++KLIETG+AI YSEPEK V SRSGDEC+VALTDQWYITY
Sbjct: 481 GTMLIGEFFGRKVQEIKPIIKTKLIETGEAIIYSEPEKPVMSRSGDECVVALTDQWYITY 540
Query: 559 GESEWKKLAEDCLANMNLFSDETRHGFQHTLSWLNQWACSRSFGLGTRIPWDEQFLVESL 618
GESEW+K+AE+CL+ MNL+SDETRHGF+HTLSWLNQWACSRSFGLGTRIPWDEQFLVESL
Sbjct: 541 GESEWRKIAEECLSKMNLYSDETRHGFEHTLSWLNQWACSRSFGLGTRIPWDEQFLVESL 600
Query: 619 SDSTIYMAYYTIAHLLHNGDLYGSGASAIKPEQMTDEVWDFVFCGAAEPKSTGISRSILN 678
SDS++YMAYYT+AH+ H+GD+Y S I+P+QM DEVW+++FC PKS+ I ++L+
Sbjct: 601 SDSSLYMAYYTVAHIFHDGDMYKGSKSLIRPQQMNDEVWEYLFCDGPYPKSSDIPSAVLS 660
Query: 679 KMKQEFEYWYPFDLRVSGKDLIQNHLTFCIYNHTAIMPEHHWPRGFRCNGHIMLNSEKMS 738
+MKQEF+YWYP DLRVSGKDLIQNHLTF IYNHTA+M +WPRG RCNGHIMLNSEKMS
Sbjct: 661 EMKQEFDYWYPLDLRVSGKDLIQNHLTFFIYNHTALMANRNWPRGIRCNGHIMLNSEKMS 720
Query: 739 KSTGNFRTLRQAIEEFSADATRFSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDIL 798
KSTGNFRTLRQ+IEEFSA TRF LADAGDGVDDANF FETANAAILRLTKE+ WME++L
Sbjct: 721 KSTGNFRTLRQSIEEFSATGTRFCLADAGDGVDDANFAFETANAAILRLTKELTWMEEVL 780
Query: 799 QADSSSFLRTGPPSTYADRVFENEINIAVKTTEQNYKDYMFREALKSGFYDLQAARDEYR 858
+SS LRTGPPSTYAD+VFEN++NIA++ TE+ YKD +FREALK+GFYDLQAARDEYR
Sbjct: 781 DVESS--LRTGPPSTYADKVFENDMNIALRLTERAYKDCLFREALKNGFYDLQAARDEYR 840
Query: 859 FSCGAGGMNRDLVFRFMDVQTRLITPICPHYAEYVWREMLKKEGFVVNAGWPSAGSPDLT 918
SCG GGM+ DL+ +FMDVQTRLI PICPH+A+YVWR++L KEG V+ AGWP + PDL
Sbjct: 841 LSCGTGGMHHDLLLKFMDVQTRLIVPICPHFADYVWRKVLNKEGCVLTAGWPPSNEPDLV 900
Query: 919 LKSANKYLQDSIVLMRKLLQKQLLGSKKGNKKGAPVTSVIEDKKLTGLIYVNEQFDGWKA 978
LKSANKYLQDSIVLMRK T+V E KL GL+YVNEQFDGW+A
Sbjct: 901 LKSANKYLQDSIVLMRKXXXXXXXXXXXXXXXXXXXTAVPEG-KLKGLVYVNEQFDGWRA 960
Query: 979 ECLRILRCKFDSTKRTFAPDSEIMEALQKSSVGQASDFRQTQKLCMPFLRFKKDEVAALG 1038
CLRIL+ +FD +F PD+E++ L + + + + + QK+CMPFL+FKKDE ++G
Sbjct: 961 HCLRILQSRFDQQTCSFPPDTEMLAELSATLLQEGKNLKAIQKVCMPFLKFKKDEAISIG 1020
Query: 1039 VHALDLKLPFGEIEVLKENLELIKRQIGLEEVEVLCASDTDAIAKAGALASLLKQNPPSP 1098
AL+L+LPFGEIEVL+ N +LI+RQ+GLEEVE+ ASD D ++ AG ASLL QNPPSP
Sbjct: 1021 TQALNLRLPFGEIEVLQSNKDLIRRQLGLEEVEIYSASDPDDVSIAGPHASLLTQNPPSP 1080
Query: 1099 GNPTAIFLTS 1109
G+PTAIF+TS
Sbjct: 1081 GSPTAIFVTS 1084
BLAST of Carg12321 vs. TAIR10
Match:
AT2G31170.1 (Cysteinyl-tRNA synthetase, class Ia family protein)
HSP 1 Score: 48.5 bits (114), Expect = 3.0e-05
Identity = 38/127 (29.92%), Postives = 58/127 (45.67%), Query Frame = 0
Query: 688 YPFDLRVSGKDLIQNHLTFCIYNHTAIMPEHHWPRGFRCNGHIMLNSEKMSKSTGNFRTL 747
Y FD+ G DL+ H I A + NG + ++SEKMSKS GNF T+
Sbjct: 281 YSFDIHGGGMDLVFPHHENEIAQSCAACDSSNISYWIH-NGFVTVDSEKMSKSLGNFFTI 340
Query: 748 RQAIEEFSADATRFSLADA--GDGVDDANFVFETANAAILRLTKEIAWMEDILQADSSSF 807
RQ I+ + A R L ++ ++F+ E+A+ I + + + E L S+F
Sbjct: 341 RQVIDLYHPLALRLFLMGTHYRSPINYSDFLLESASERIFYIYQTLHDCESALGEKDSTF 400
Query: 808 LRTGPPS 813
PS
Sbjct: 401 ENGSVPS 406
BLAST of Carg12321 vs. TAIR10
Match:
AT5G49030.1 (tRNA synthetase class I (I, L, M and V) family protein)
HSP 1 Score: 46.2 bits (108), Expect = 1.5e-04
Identity = 29/91 (31.87%), Postives = 43/91 (47.25%), Query Frame = 0
Query: 27 FARRDSLREIESKVRVLWEENDVFRAEACETPPKEGEKFFGNFPFPYMNGFLHLGHAFSL 86
F R + E +++ LWEEN VF+ + G F + PY NG LH+GHA +
Sbjct: 113 FGMRANSLTREPELQKLWEENQVFKR---VSDNNNGGSFILHDGPPYANGDLHMGHALNK 172
Query: 87 SKLEFAAAYHRLRGANVLLPFGFHCTGMPIK 118
+ Y L+ V G+ C G+PI+
Sbjct: 173 ILKDIINRYKLLQNYKVQYVPGWDCHGLPIE 200
BLAST of Carg12321 vs. Swiss-Prot
Match:
sp|F4I116|SYLC_ARATH (Leucine--tRNA ligase, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At1g09620 PE=1 SV=1)
HSP 1 Score: 1652.9 bits (4279), Expect = 0.0e+00
Identity = 782/1090 (71.74%), Postives = 932/1090 (85.50%), Query Frame = 0
Query: 19 MASECGKSFARRDSLREIESKVRVLWEENDVFRAEACETPPKEGEKFFGNFPFPYMNGFL 78
MASE KS+ARRD L EIE+ VR WE+ DVFRAE+CE PK GEKFF FPFPYMNG+L
Sbjct: 1 MASE-SKSYARRDRLLEIEATVRKWWEDEDVFRAESCENLPKPGEKFFSTFPFPYMNGYL 60
Query: 79 HLGHAFSLSKLEFAAAYHRLRGANVLLPFGFHCTGMPIKASADKLAREIQQFGDPPVFPR 138
H+GHAFSLSK++FA+AYHRLRGANVLLPFGFHCTGMPIKASADKL REI+QFG+PPVF
Sbjct: 61 HIGHAFSLSKVDFASAYHRLRGANVLLPFGFHCTGMPIKASADKLRREIEQFGNPPVFTA 120
Query: 139 QMEEQENLKAESEDANESNPTLPDKFKGKKSKAVSKSSGQMYQWEIMRSFGLSDSEISKF 198
+ + + + + E ++ +P +FKGKKSK +K+ GQ+YQWEIMRSFGL+DSEI+ F
Sbjct: 121 E-DTTKVPEVQEESSDTIALPIPGQFKGKKSKVAAKAGGQVYQWEIMRSFGLTDSEIANF 180
Query: 199 QNPYNWLTFFPPLAMEDLKAFGLGCDWRRSFITTDMNPFFDSFVQWQMRKLKSMGKIVKD 258
+ P WL +FPPLA+EDL+A+GLGCDWRRSF+TTD+NPFFD+FV+WQMRKLKSMGKIVKD
Sbjct: 181 REPSEWLYYFPPLAVEDLRAYGLGCDWRRSFVTTDVNPFFDAFVRWQMRKLKSMGKIVKD 240
Query: 259 VRYTIYSPLDGQPCADHDRASGEGVQPQDYTLIKMEVVPPFPPKMGVLEGRKVYLAAATL 318
RYTI+SPLDGQPCADHDRA+GEGVQPQ+YTLIKMEVV PFP K+G LEG++V+LAAATL
Sbjct: 241 RRYTIFSPLDGQPCADHDRATGEGVQPQEYTLIKMEVVKPFPLKLGPLEGKRVFLAAATL 300
Query: 319 RPETMYGQTNAWVLPEGKYGAFEINDTDVFIITERAALNLAYQKFSRVPEKPTCLIQLTG 378
RPETMYGQTNAWVLP+GKYGA+EI++T+VFI+TERAALNLAYQ FS+ P++P+CL++LTG
Sbjct: 301 RPETMYGQTNAWVLPDGKYGAYEISETEVFILTERAALNLAYQNFSKNPQEPSCLVELTG 360
Query: 379 NDLIGLPLKSPLAFNEIIYALPMLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRA 438
DLIGLPL+SPL+ NEIIYALPMLTILT+KGTGIVTSVPSDAPDDYMA+ DL KPAL+
Sbjct: 361 YDLIGLPLRSPLSVNEIIYALPMLTILTNKGTGIVTSVPSDAPDDYMALQDLIKKPALQD 420
Query: 439 KYGVKDEWVLPYDIVPIIDIPEFGDRAAEKVCVDLKIKSQNEKDKLAEAKRLTYLKGFTD 498
KYGVK EW LP +I+PII+IPEFGD+AAEKVC+DLKIKSQN+K+KLAEAKRLTYLKGFT+
Sbjct: 421 KYGVKTEW-LPTEIIPIINIPEFGDKAAEKVCLDLKIKSQNDKEKLAEAKRLTYLKGFTE 480
Query: 499 GTLIVGEFSGRKVQEAKPMIRSKLIETGQAIPYSEPEKRVTSRSGDECIVALTDQWYITY 558
GT+++GEF GRKVQE KP+I++KLIETG+AI YSEPEK V SRSGDEC+VALTDQWYITY
Sbjct: 481 GTMLIGEFFGRKVQEIKPIIKTKLIETGEAIIYSEPEKPVMSRSGDECVVALTDQWYITY 540
Query: 559 GESEWKKLAEDCLANMNLFSDETRHGFQHTLSWLNQWACSRSFGLGTRIPWDEQFLVESL 618
GESEW+K+AE+CL+ MNL+SDETRHGF+HTLSWLNQWACSRSFGLGTRIPWDEQFLVESL
Sbjct: 541 GESEWRKIAEECLSKMNLYSDETRHGFEHTLSWLNQWACSRSFGLGTRIPWDEQFLVESL 600
Query: 619 SDSTIYMAYYTIAHLLHNGDLYGSGASAIKPEQMTDEVWDFVFCGAAEPKSTGISRSILN 678
SDS++YMAYYT+AH+ H+GD+Y S I+P+QM DEVW+++FC PKS+ I ++L+
Sbjct: 601 SDSSLYMAYYTVAHIFHDGDMYKGSKSLIRPQQMNDEVWEYLFCDGPYPKSSDIPSAVLS 660
Query: 679 KMKQEFEYWYPFDLRVSGKDLIQNHLTFCIYNHTAIMPEHHWPRGFRCNGHIMLNSEKMS 738
+MKQEF+YWYP DLRVSGKDLIQNHLTF IYNHTA+M +WPRG RCNGHIMLNSEKMS
Sbjct: 661 EMKQEFDYWYPLDLRVSGKDLIQNHLTFFIYNHTALMANRNWPRGIRCNGHIMLNSEKMS 720
Query: 739 KSTGNFRTLRQAIEEFSADATRFSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDIL 798
KSTGNFRTLRQ+IEEFSA TRF LADAGDGVDDANF FETANAAILRLTKE+ WME++L
Sbjct: 721 KSTGNFRTLRQSIEEFSATGTRFCLADAGDGVDDANFAFETANAAILRLTKELTWMEEVL 780
Query: 799 QADSSSFLRTGPPSTYADRVFENEINIAVKTTEQNYKDYMFREALKSGFYDLQAARDEYR 858
+SS LRTGPPSTYAD+VFEN++NIA++ TE+ YKD +FREALK+GFYDLQAARDEYR
Sbjct: 781 DVESS--LRTGPPSTYADKVFENDMNIALRLTERAYKDCLFREALKNGFYDLQAARDEYR 840
Query: 859 FSCGAGGMNRDLVFRFMDVQTRLITPICPHYAEYVWREMLKKEGFVVNAGWPSAGSPDLT 918
SCG GGM+ DL+ +FMDVQTRLI PICPH+A+YVWR++L KEG V+ AGWP + PDL
Sbjct: 841 LSCGTGGMHHDLLLKFMDVQTRLIVPICPHFADYVWRKVLNKEGCVLTAGWPPSNEPDLV 900
Query: 919 LKSANKYLQDSIVLMRKLLQKQLLGSKKGNKKGAPVTSVIEDKKLTGLIYVNEQFDGWKA 978
LKSANKYLQDSIVLMRK T+V E KL GL+YVNEQFDGW+A
Sbjct: 901 LKSANKYLQDSIVLMRKXXXXXXXXXXXXXXXXXXXTAVPEG-KLKGLVYVNEQFDGWRA 960
Query: 979 ECLRILRCKFDSTKRTFAPDSEIMEALQKSSVGQASDFRQTQKLCMPFLRFKKDEVAALG 1038
CLRIL+ +FD +F PD+E++ L + + + + + QK+CMPFL+FKKDE ++G
Sbjct: 961 HCLRILQSRFDQQTCSFPPDTEMLAELSATLLQEGKNLKAIQKVCMPFLKFKKDEAISIG 1020
Query: 1039 VHALDLKLPFGEIEVLKENLELIKRQIGLEEVEVLCASDTDAIAKAGALASLLKQNPPSP 1098
AL+L+LPFGEIEVL+ N +LI+RQ+GLEEVE+ ASD D ++ AG ASLL QNPPSP
Sbjct: 1021 TQALNLRLPFGEIEVLQSNKDLIRRQLGLEEVEIYSASDPDDVSIAGPHASLLTQNPPSP 1080
Query: 1099 GNPTAIFLTS 1109
G+PTAIF+TS
Sbjct: 1081 GSPTAIFVTS 1084
BLAST of Carg12321 vs. Swiss-Prot
Match:
sp|Q8BMJ2|SYLC_MOUSE (Leucine--tRNA ligase, cytoplasmic OS=Mus musculus OX=10090 GN=Lars PE=1 SV=2)
HSP 1 Score: 1046.6 bits (2705), Expect = 2.0e-304
Identity = 526/1090 (48.26%), Postives = 713/1090 (65.41%), Query Frame = 0
Query: 25 KSFARRDSLREIESKVRVLWEENDVFRAEACETPPKEGE---KFFGNFPFPYMNGFLHLG 84
K A+ D L+EIE + + WE VF A ++ K+F FP+PYMNG LHLG
Sbjct: 5 KGTAKVDFLKEIEKEAQQKWEAEKVFEVSASRLEKQKQSSKGKYFVTFPYPYMNGRLHLG 64
Query: 85 HAFSLSKLEFAAAYHRLRGANVLLPFGFHCTGMPIKASADKLAREIQQFGDPPVFPRQME 144
H FSLSK EFA Y RL+G + L PFG HCTGMPIKA ADKL REI+ +G PP FP
Sbjct: 65 HTFSLSKCEFAVGYQRLKGKSCLFPFGLHCTGMPIKACADKLKREIELYGCPPDFPXXXX 124
Query: 145 EQENLKAESEDANESNPTLPDKFKGKKSKAVSKSSGQMYQWEIMRSFGLSDSEISKFQNP 204
D + DK KGKKSKA +K+ YQW+IM+S GLSD +I KF
Sbjct: 125 XXXXXXXXXXDI-----VVRDKAKGKKSKAAAKAGSSKYQWDIMKSLGLSDDDIVKFSEA 184
Query: 205 YNWLTFFPPLAMEDLKAFGLGCDWRRSFITTDMNPFFDSFVQWQMRKLKSMGKIVKDVRY 264
+WL +FPPLA++DLK GL DWRRSFITTD+NP++DSFV+WQ L+ KI RY
Sbjct: 185 EHWLDYFPPLAVQDLKTIGLKVDWRRSFITTDVNPYYDSFVRWQFLTLRERNKIKFGKRY 244
Query: 265 TIYSPLDGQPCADHDRASGEGVQPQDYTLIKMEVVPPFPPKMGVLEGRKVYLAAATLRPE 324
TIYSP DGQPC DHDR +GEGV PQ+YTL+K++V+ P+P K+ L+G+ ++L AATLRPE
Sbjct: 245 TIYSPKDGQPCMDHDRQTGEGVGPQEYTLVKLKVLEPYPSKLSGLKGKNIFLVAATLRPE 304
Query: 325 TMYGQTNAWVLPEGKYGAFEINDTDVFIITERAALNLAYQKFSRVPEKPTCLIQLTGNDL 384
TM+GQTN WV P+ KY FE + D+FI T+RAA N++YQ F++ + +L G ++
Sbjct: 305 TMFGQTNCWVRPDMKYIGFETANGDIFICTQRAARNMSYQGFTKHNGVVPVVKELMGEEI 364
Query: 385 IGLPLKSPLAFNEIIYALPMLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRAKYG 444
+G L +PL +++Y LPMLTI DKGTG+VTSVPSD+PDD A+ DLK K ALR K+G
Sbjct: 365 LGASLSAPLTCYKVVYVLPMLTIKEDKGTGVVTSVPSDSPDDLAALRDLKKKQALRTKFG 424
Query: 445 VKDEWVLPYDIVPIIDIPEFGDRAAEKVCVDLKIKSQNEKDKLAEAKRLTYLKGFTDGTL 504
++D+ VLP++ VP+++IP G+ A VC +LKI+SQN+++KLAEAK YL+GF DG +
Sbjct: 425 IRDDMVLPFEPVPVLEIPGIGNLPAVTVCDELKIQSQNDREKLAEAKEKLYLRGFYDGVM 484
Query: 505 IVGEFSGRKVQEAKPMIRSKLIETGQAIPYSEPEKRVTSRSGDECIVALTDQWYITYGES 564
+V F G+K+Q K I+ +I+ G A+ Y EPEK+V SRS DEC+VAL DQWY+ YG+
Sbjct: 485 LVDGFKGQKIQHVKKTIQKNMIDAGDALIYMEPEKQVMSRSADECVVALCDQWYLDYGDE 544
Query: 565 EWKKLAEDCLANMNLFSDETRHGFQHTLSWLNQWACSRSFGLGTRIPWDEQFLVESLSDS 624
WKK CL NM F +E+R F+ +L WL + ACSR++GLGTR+PWDEQ+L+ESLSDS
Sbjct: 545 NWKKQTFQCLKNMETFCEESRKNFEASLDWLQEHACSRTYGLGTRLPWDEQWLIESLSDS 604
Query: 625 TIYMAYYTIAHLLHNGDLYGSGAS--AIKPEQMTDEVWDFVFCGAAEPKSTGISRSILNK 684
TIYMA+YT+AHLL GDL G S I+P+QMT +VWD+VF A T I + L++
Sbjct: 605 TIYMAFYTVAHLLQGGDLNGQAESPLGIRPQQMTKDVWDYVFFKDAPFPKTQIPKEKLDQ 664
Query: 685 MKQEFEYWYPFDLRVSGKDLIQNHLTFCIYNHTAIMPEH--HWPRGFRCNGHIMLNSEKM 744
+KQEFE+WYP DLR SGKDLI NHL++ IYNH A+ PE WP R NGH++LNSEKM
Sbjct: 665 LKQEFEFWYPVDLRASGKDLIPNHLSYYIYNHVAMWPEQSDKWPVSVRANGHLLLNSEKM 724
Query: 745 SKSTGNFRTLRQAIEEFSADATRFSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDI 804
SKSTGNF TL QA+++FSAD R +LADAGD V+DANFV A+A ILRL + W++++
Sbjct: 725 SKSTGNFLTLSQAVDKFSADGMRLALADAGDTVEDANFVEAMADAGILRLYTWVEWVKEM 784
Query: 805 LQADSSSFLRTGPPSTYADRVFENEINIAVKTTEQNYKDYMFREALKSGFYDLQAARDEY 864
L + SS LR+GP ++ DRVF +E+N + T+QNY+ MF+EALK+GF++ QAA+D+Y
Sbjct: 785 LASCSS--LRSGPADSFNDRVFASEMNAGIIKTDQNYEKMMFKEALKTGFFEFQAAKDKY 844
Query: 865 RFSCGAGGMNRDLVFRFMDVQTRLITPICPHYAEYVWREMLKKEGFVVNAGWPSAGSPDL 924
R GM+R+LVFRF++VQT L+TP CPH E++W +L K +++A WP AG D
Sbjct: 845 R-ELATEGMHRELVFRFIEVQTILLTPFCPHLCEHIW-TLLGKPDSIMHASWPVAGPVDE 904
Query: 925 TLKSANKYLQDSIVLMRKLLQKQLLGSKKGNKKGAPVTSVIEDKKLTGLIYVNEQFDGWK 984
+L +++YL + +R L+ ++ +K P + IYV + + W+
Sbjct: 905 SLIRSSQYLMEVAHDLRLRLKNYMMPAKGKKTDKQPA-----QRPSHCTIYVAKNYPVWQ 964
Query: 985 AECLRILRCKFDSTKRTFAPDSEIMEALQKSSVGQASDFRQTQKLCMPFLRFKKDEVAAL 1044
L LR F++ PD++++ S +G + ++ K MPF+ K+ +
Sbjct: 965 HITLTTLRSHFEANNGKL-PDNKVI----ASELGSLPELKKYMKKVMPFVAMIKENMEKK 1024
Query: 1045 GVHALDLKLPFGEIEVLKENLELIKRQIGLEEVEVLCASDTDAIAKAGALASLLKQNPPS 1104
G LDL+L F E VL EN+ + + LE +EV AS+ + +
Sbjct: 1025 GPRVLDLELEFDEQAVLMENIVYLTNSLELEHIEVKFASEAEDKVREEC----------C 1065
Query: 1105 PGNPTAIFLT 1108
PG P +F T
Sbjct: 1085 PGKPLNVFRT 1065
BLAST of Carg12321 vs. Swiss-Prot
Match:
sp|Q5R614|SYLC_PONAB (Leucine--tRNA ligase, cytoplasmic OS=Pongo abelii OX=9601 GN=LARS PE=2 SV=1)
HSP 1 Score: 1036.6 bits (2679), Expect = 2.0e-301
Identity = 517/1060 (48.77%), Postives = 702/1060 (66.23%), Query Frame = 0
Query: 25 KSFARRDSLREIESKVRVLWEENDVFRAEACETPPKEGE-KFFGNFPFPYMNGFLHLGHA 84
K A+ D L++IE +++ W+ VF A + + K+F FP+PYMNG LHLGH
Sbjct: 5 KGTAKVDFLKKIEKEIQQKWDTERVFEVNASSLEKQTSKGKYFVTFPYPYMNGRLHLGHT 64
Query: 85 FSLSKLEFAAAYHRLRGANVLLPFGFHCTGMPIKASADKLAREIQQFGDPPVFPRQMEEQ 144
FSLSK EFA Y RL+G L PFG HCTGMPIKA ADKL REI+ +G PP FP +
Sbjct: 65 FSLSKCEFAVGYQRLKGKCCLFPFGLHCTGMPIKACADKLKREIELYGCPPDFPDEXXXX 124
Query: 145 ENLKAESEDANESNPTLPDKFKGKKSKAVSKSSGQMYQWEIMRSFGLSDSEISKFQNPYN 204
SK YQW IM+S GLSD EI KF +
Sbjct: 125 XXXXXXXXXXXXXXXXXXXXXXXXXXXGSSK-----YQWGIMKSLGLSDEEIVKFSEAEH 184
Query: 205 WLTFFPPLAMEDLKAFGLGCDWRRSFITTDMNPFFDSFVQWQMRKLKSMGKIVKDVRYTI 264
WL +FPPLA++DLK GL DWRRSFITTD+NP++DSFV+WQ L+ KI RYTI
Sbjct: 185 WLDYFPPLAIQDLKRLGLKVDWRRSFITTDVNPYYDSFVRWQFLTLRERNKIKFGKRYTI 244
Query: 265 YSPLDGQPCADHDRASGEGVQPQDYTLIKMEVVPPFPPKMGVLEGRKVYLAAATLRPETM 324
YSP DGQPC DHDR +GEGV PQ+YTL+K++V+ P+P K+ L+G+ ++L AATLRPET+
Sbjct: 245 YSPKDGQPCMDHDRQTGEGVGPQEYTLLKLKVLEPYPSKLSGLKGKNIFLVAATLRPETL 304
Query: 325 YGQTNAWVLPEGKYGAFEINDTDVFIITERAALNLAYQKFSRVPEKPTCLIQLTGNDLIG 384
+GQTN WV P+ KY FE + D+FI T++AA N++YQ F++ + +L G +++G
Sbjct: 305 FGQTNCWVRPDMKYIGFETVNGDIFICTQKAARNMSYQGFTKDNGVVPVVKELMGEEILG 364
Query: 385 LPLKSPLAFNEIIYALPMLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRAKYGVK 444
L +PL ++IY LPMLTI DKGTG+VTSVPSD+PDD A+ DLK K ALRAKYG++
Sbjct: 365 ASLSAPLTSYKVIYVLPMLTIKEDKGTGVVTSVPSDSPDDIAALRDLKKKQALRAKYGIR 424
Query: 445 DEWVLPYDIVPIIDIPEFGDRAAEKVCVDLKIKSQNEKDKLAEAKRLTYLKGFTDGTLIV 504
D+ VLP++ VP+I+IP FG+ +A +C +LKI+SQN+++KLAEAK YLKGF +G ++V
Sbjct: 425 DDMVLPFEPVPVIEIPGFGNLSAVTICDELKIQSQNDREKLAEAKEKIYLKGFYEGIMLV 484
Query: 505 GEFSGRKVQEAKPMIRSKLIETGQAIPYSEPEKRVTSRSGDECIVALTDQWYITYGESEW 564
F G+KVQ+ K I+ K+I+ G A+ Y EPEK+V SRS DEC+VAL DQWY+ YGE W
Sbjct: 485 DGFKGQKVQDVKKTIQKKMIDAGDALIYMEPEKQVMSRSSDECVVALCDQWYLDYGEENW 544
Query: 565 KKLAEDCLANMNLFSDETRHGFQHTLSWLNQWACSRSFGLGTRIPWDEQFLVESLSDSTI 624
KK CL N+ F +ETR F+ TL WL + ACSR++GLGT +PWDEQ+L+ESLSDSTI
Sbjct: 545 KKQTSQCLKNLETFCEETRRNFEATLGWLQEHACSRTYGLGTHLPWDEQWLIESLSDSTI 604
Query: 625 YMAYYTIAHLLHNGDLYGSGAS--AIKPEQMTDEVWDFVFCGAAEPKSTGISRSILNKMK 684
YMA+YT+AHLL G+L+G S I+P+QMT EVWD+VF A T I++ L+++K
Sbjct: 605 YMAFYTVAHLLQGGNLHGQAESPLGIRPQQMTKEVWDYVFFKEAPFPKTQIAKEKLDQLK 664
Query: 685 QEFEYWYPFDLRVSGKDLIQNHLTFCIYNHTAIMPEH--HWPRGFRCNGHIMLNSEKMSK 744
QEFE+WYP DLRVSGKDL+ NHL++ +YNH A+ PE WP R NGH++LNSEKMSK
Sbjct: 665 QEFEFWYPVDLRVSGKDLVPNHLSYYLYNHVAMWPEQSDKWPTAVRANGHLLLNSEKMSK 724
Query: 745 STGNFRTLRQAIEEFSADATRFSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDILQ 804
STGNF TL QAI++FSAD R +LADAGD V+DANFV A+A ILRL + W+++++
Sbjct: 725 STGNFLTLTQAIDKFSADGMRLALADAGDTVEDANFVEAMADAGILRLYTWVEWVKEMVA 784
Query: 805 ADSSSFLRTGPPSTYADRVFENEINIAVKTTEQNYKDYMFREALKSGFYDLQAARDEYRF 864
S LR+GP +T+ DRVF +E+N + T+QNY+ MF+EALK+GF++ QAA+D+YR
Sbjct: 785 NWDS--LRSGPANTFNDRVFASELNAGIIKTDQNYEKMMFKEALKTGFFEFQAAKDKYR- 844
Query: 865 SCGAGGMNRDLVFRFMDVQTRLITPICPHYAEYVWREMLKKEGFVVNAGWPSAGSPDLTL 924
GM+R+LVFRF++VQT L+ P CPH E++W +L K ++NA WP AG D L
Sbjct: 845 ELAVEGMHRELVFRFIEVQTLLLAPFCPHLCEHIW-TLLGKPDSIMNASWPVAGPVDEVL 904
Query: 925 KSANKYLQDSIVLMRKLLQKQLLGSKKGNKKGAPVTSVIEDKKLTGLIYVNEQFDGWKAE 984
+++YL + +R L+ ++ +K P+ K IYV + + W+
Sbjct: 905 IHSSQYLMEVTHDLRLRLKNYMMPAKGKKTDKQPL-----QKPSHCTIYVAKNYPPWQHT 964
Query: 985 CLRILRCKFDSTKRTFAPDSEIMEALQKSSVGQASDFRQTQKLCMPFLRFKKDEVAALGV 1044
L +LR F++ R PD++++ S +G + ++ K MPF+ K+ + +G
Sbjct: 965 TLSVLRKHFEANNRKL-PDNKVI----ASELGSMPELKKYMKKVMPFVAMIKENLEKMGP 1024
Query: 1045 HALDLKLPFGEIEVLKENLELIKRQIGLEEVEVLCASDTD 1080
LDL+L F E VL EN+ + + LE +EV AS+ +
Sbjct: 1025 RILDLQLEFDEKAVLMENIVYLTNSLELEHIEVKFASEAE 1045
BLAST of Carg12321 vs. Swiss-Prot
Match:
sp|Q9P2J5|SYLC_HUMAN (Leucine--tRNA ligase, cytoplasmic OS=Homo sapiens OX=9606 GN=LARS PE=1 SV=2)
HSP 1 Score: 1033.1 bits (2670), Expect = 2.3e-300
Identity = 517/1060 (48.77%), Postives = 700/1060 (66.04%), Query Frame = 0
Query: 25 KSFARRDSLREIESKVRVLWEENDVFRAEACETPPKEGE-KFFGNFPFPYMNGFLHLGHA 84
K A+ D L++IE +++ W+ VF A + + K+F FP+PYMNG LHLGH
Sbjct: 5 KGTAKVDFLKKIEKEIQQKWDTERVFEVNASNLEKQTSKGKYFVTFPYPYMNGRLHLGHT 64
Query: 85 FSLSKLEFAAAYHRLRGANVLLPFGFHCTGMPIKASADKLAREIQQFGDPPVFPRQMEEQ 144
FSLSK EFA Y RL+G L PFG HCTGMPIKA ADKL REI+ +G PP FP
Sbjct: 65 FSLSKCEFAVGYQRLKGKCCLFPFGLHCTGMPIKACADKLKREIELYGCPPDFPDXXXXX 124
Query: 145 ENLKAESEDANESNPTLPDKFKGKKSKAVSKSSGQMYQWEIMRSFGLSDSEISKFQNPYN 204
SK YQW IM+S GLSD EI KF +
Sbjct: 125 XXXXXXXXXXXXXXXXXXXXXXXXXXXGSSK-----YQWGIMKSLGLSDEEIVKFSEAEH 184
Query: 205 WLTFFPPLAMEDLKAFGLGCDWRRSFITTDMNPFFDSFVQWQMRKLKSMGKIVKDVRYTI 264
WL +FPPLA++DLK GL DWRRSFITTD+NP++DSFV+WQ L+ KI RYTI
Sbjct: 185 WLDYFPPLAIQDLKRMGLKVDWRRSFITTDVNPYYDSFVRWQFLTLRERNKIKFGKRYTI 244
Query: 265 YSPLDGQPCADHDRASGEGVQPQDYTLIKMEVVPPFPPKMGVLEGRKVYLAAATLRPETM 324
YSP DGQPC DHDR +GEGV PQ+YTL+K++V+ P+P K+ L+G+ ++L AATLRPETM
Sbjct: 245 YSPKDGQPCMDHDRQTGEGVGPQEYTLLKLKVLEPYPSKLSGLKGKNIFLVAATLRPETM 304
Query: 325 YGQTNAWVLPEGKYGAFEINDTDVFIITERAALNLAYQKFSRVPEKPTCLIQLTGNDLIG 384
+GQTN WV P+ KY FE + D+FI T++AA N++YQ F++ + +L G +++G
Sbjct: 305 FGQTNCWVRPDMKYIGFETVNGDIFICTQKAARNMSYQGFTKDNGVVPVVKELMGEEILG 364
Query: 385 LPLKSPLAFNEIIYALPMLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRAKYGVK 444
L +PL ++IY LPMLTI DKGTG+VTSVPSD+PDD A+ DLK K ALRAKYG++
Sbjct: 365 ASLSAPLTSYKVIYVLPMLTIKEDKGTGVVTSVPSDSPDDIAALRDLKKKQALRAKYGIR 424
Query: 445 DEWVLPYDIVPIIDIPEFGDRAAEKVCVDLKIKSQNEKDKLAEAKRLTYLKGFTDGTLIV 504
D+ VLP++ VP+I+IP FG+ +A +C +LKI+SQN+++KLAEAK YLKGF +G ++V
Sbjct: 425 DDMVLPFEPVPVIEIPGFGNLSAVTICDELKIQSQNDREKLAEAKEKIYLKGFYEGIMLV 484
Query: 505 GEFSGRKVQEAKPMIRSKLIETGQAIPYSEPEKRVTSRSGDECIVALTDQWYITYGESEW 564
F G+KVQ+ K I+ K+I+ G A+ Y EPEK+V SRS DEC+VAL DQWY+ YGE W
Sbjct: 485 DGFKGQKVQDVKKTIQKKMIDAGDALIYMEPEKQVMSRSSDECVVALCDQWYLDYGEENW 544
Query: 565 KKLAEDCLANMNLFSDETRHGFQHTLSWLNQWACSRSFGLGTRIPWDEQFLVESLSDSTI 624
KK CL N+ F +ETR F+ TL WL + ACSR++GLGT +PWDEQ+L+ESLSDSTI
Sbjct: 545 KKQTSQCLKNLETFCEETRRNFEATLGWLQEHACSRTYGLGTHLPWDEQWLIESLSDSTI 604
Query: 625 YMAYYTIAHLLHNGDLYGSGAS--AIKPEQMTDEVWDFVFCGAAEPKSTGISRSILNKMK 684
YMA+YT+AHLL G+L+G S I+P+QMT EVWD+VF A T I++ L+++K
Sbjct: 605 YMAFYTVAHLLQGGNLHGQAESPLGIRPQQMTKEVWDYVFFKEAPFPKTQIAKEKLDQLK 664
Query: 685 QEFEYWYPFDLRVSGKDLIQNHLTFCIYNHTAIMPEH--HWPRGFRCNGHIMLNSEKMSK 744
QEFE+WYP DLRVSGKDL+ NHL++ +YNH A+ PE WP R NGH++LNSEKMSK
Sbjct: 665 QEFEFWYPVDLRVSGKDLVPNHLSYYLYNHVAMWPEQSDKWPTAVRANGHLLLNSEKMSK 724
Query: 745 STGNFRTLRQAIEEFSADATRFSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDILQ 804
STGNF TL QAI++FSAD R +LADAGD V+DANFV A+A ILRL + W+++++
Sbjct: 725 STGNFLTLTQAIDKFSADGMRLALADAGDTVEDANFVEAMADAGILRLYTWVEWVKEMVA 784
Query: 805 ADSSSFLRTGPPSTYADRVFENEINIAVKTTEQNYKDYMFREALKSGFYDLQAARDEYRF 864
S LR+GP ST+ DRVF +E+N + T+QNY+ MF+EALK+GF++ QAA+D+YR
Sbjct: 785 NWDS--LRSGPASTFNDRVFASELNAGIIKTDQNYEKMMFKEALKTGFFEFQAAKDKYR- 844
Query: 865 SCGAGGMNRDLVFRFMDVQTRLITPICPHYAEYVWREMLKKEGFVVNAGWPSAGSPDLTL 924
GM+R+LVFRF++VQT L+ P CPH E++W +L K ++NA WP AG + L
Sbjct: 845 ELAVEGMHRELVFRFIEVQTLLLAPFCPHLCEHIW-TLLGKPDSIMNASWPVAGPVNEVL 904
Query: 925 KSANKYLQDSIVLMRKLLQKQLLGSKKGNKKGAPVTSVIEDKKLTGLIYVNEQFDGWKAE 984
+++YL + +R L+ ++ +K P+ K IYV + + W+
Sbjct: 905 IHSSQYLMEVTHDLRLRLKNYMMPAKGKKTDKQPL-----QKPSHCTIYVAKNYPPWQHT 964
Query: 985 CLRILRCKFDSTKRTFAPDSEIMEALQKSSVGQASDFRQTQKLCMPFLRFKKDEVAALGV 1044
L +LR F++ PD++++ S +G + ++ K MPF+ K+ + +G
Sbjct: 965 TLSVLRKHFEANNGKL-PDNKVI----ASELGSMPELKKYMKKVMPFVAMIKENLEKMGP 1024
Query: 1045 HALDLKLPFGEIEVLKENLELIKRQIGLEEVEVLCASDTD 1080
LDL+L F E VL EN+ + + LE +EV AS+ +
Sbjct: 1025 RILDLQLEFDEKAVLMENIVYLTNSLELEHIEVKFASEAE 1045
BLAST of Carg12321 vs. Swiss-Prot
Match:
sp|Q09996|SYLC_CAEEL (Leucine--tRNA ligase OS=Caenorhabditis elegans OX=6239 GN=lars-1 PE=3 SV=2)
HSP 1 Score: 1007.7 bits (2604), Expect = 1.0e-292
Identity = 515/1065 (48.36%), Postives = 693/1065 (65.07%), Query Frame = 0
Query: 21 SECGKSFARRDSLREIESKVRVLWEENDVFRAEACETPPKEGE-KFFGNFPFPYMNGFLH 80
S+ K + L+EIE ++ LWE F A+A + +G+ K+ FPFPYMNG LH
Sbjct: 2 SKINKERKKVAQLQEIEKSIQELWESKKAFEADARD----DGKPKYLVTFPFPYMNGRLH 61
Query: 81 LGHAFSLSKLEFAAAYHRLRGANVLLPFGFHCTGMPIKASADKLAREIQQFGDPPVFPRQ 140
LGH FS SK EFAA + RL+G VL PFGFHCTGMPIKA ADKL RE+Q FG PP FP
Sbjct: 62 LGHTFSASKCEFAAGFQRLQGKEVLFPFGFHCTGMPIKACADKLKREMQDFGYPPNFPED 121
Query: 141 MEEQENLKAESEDANESNPTLPDKFKGKKSKAVSKSSGQMYQWEIMRSFGLSDSEISKFQ 200
+ + D + DK KGKKSK V+K+ YQW+IM+S GL D EI +F
Sbjct: 122 VVXXXXXXXSAVD-----EIIKDKSKGKKSKLVAKTGNAKYQWQIMKSLGLCDEEIKEFS 181
Query: 201 NPYNWLTFFPPLAMEDLKAFGLGCDWRRSFITTDMNPFFDSFVQWQMRKLKSMGKIVKDV 260
+P +WL +FPP + DLK GL DWRRSFITTD+NP+FDSFV+WQ L++ KI
Sbjct: 182 DPNHWLYYFPPHCIADLKKMGLKADWRRSFITTDVNPYFDSFVRWQFNLLRAAKKIDFGK 241
Query: 261 RYTIYSPLDGQPCADHDRASGEGVQPQDYTLIKMEVVPPFPPKMGVLEGRKVYLAAATLR 320
RYTIYSP DGQPC DHDRASGEGV PQ+YTLIK++V+ P P + ++ +YL AATLR
Sbjct: 242 RYTIYSPKDGQPCMDHDRASGEGVGPQEYTLIKLKVLDPKPQALSHIK-EDIYLVAATLR 301
Query: 321 PETMYGQTNAWVLPEGKYGAFEI--NDTDVFIITERAALNLAYQKFSRVPEKPTCLI--- 380
PETMYGQTN ++ P+ +Y F N+ VF+ T R+A ++YQ ++ K ++
Sbjct: 302 PETMYGQTNCYLHPDIQYSVFYATENEKQVFVATARSARIMSYQGLTKENGKVNYVLGLE 361
Query: 381 QLTGNDLIGLPLKSPLAFNEIIYALPMLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKP 440
++ G+ ++G PL +PLA E +YALPMLTI DKGTG+VTSVPSD+PDD+ A+ DLK K
Sbjct: 362 KIAGSKILGAPLSAPLAKYERVYALPMLTIKDDKGTGVVTSVPSDSPDDFAALSDLKKKK 421
Query: 441 ALRAKYGVKDEWVLPYDIVPIIDIPEFGDRAAEKVCVDLKIKSQNEKDKLAEAKRLTYLK 500
LR KYG+ DE VLP++ VPII I GD AA ++C LKI+SQNEKDKL EAK+ YLK
Sbjct: 422 PLREKYGLTDEMVLPFEPVPIIKIEGLGDLAAVEMCSRLKIESQNEKDKLEEAKKEVYLK 481
Query: 501 GFTDGTLIVGEFSGRKVQEAKPMIRSKLIETGQAIPYSEPEKRVTSRSGDECIVALTDQW 560
GF DG ++VG+++G+K + K +I+ L G A Y EPEK+V SRSGDEC+VAL DQW
Sbjct: 482 GFYDGVMLVGKYAGKKTADVKKVIQDDLTAEGLATKYVEPEKKVMSRSGDECVVALCDQW 541
Query: 561 YITYGESEWKKLAEDCLANMNLFSDETRHGFQHTLSWLNQWACSRSFGLGTRIPWDEQFL 620
Y+ YGE+EWK A+ L + F+DETR G + T+ WL+++ACSRS+GLGT++PWD Q+L
Sbjct: 542 YLNYGEAEWKAAAKKVLEPLRTFNDETRRGLETTVDWLHEYACSRSYGLGTKLPWDTQYL 601
Query: 621 VESLSDSTIYMAYYTIAHLLHNGDLYGS--GASAIKPEQMTDEVWDFVFCGAA-EPKSTG 680
+ESLSDSTIY AYYT+AHLL G GS G + IK +QMTD W +VF G + K+
Sbjct: 602 IESLSDSTIYNAYYTVAHLLQQGAFDGSVVGPAGIKADQMTDASWSYVFLGEIYDSKTMP 661
Query: 681 ISRSILNKMKQEFEYWYPFDLRVSGKDLIQNHLTFCIYNHTAIMP--EHHWPRGFRCNGH 740
+ L +++EF YWYP D+R SGKDLI NHLT+ ++NH AI P WP+G R NGH
Sbjct: 662 VEEEKLKSLRKEFMYWYPIDMRASGKDLIGNHLTYLLFNHAAIWPTDTSKWPKGIRANGH 721
Query: 741 IMLNSEKMSKSTGNFRTLRQAIEEFSADATRFSLADAGDGVDDANFVFETANAAILRLTK 800
++LN+EKMSKSTGNF TL +AIE+FSAD R SLADAGDG++DANFV+ A+AAILRL
Sbjct: 722 LLLNNEKMSKSTGNFMTLEEAIEKFSADGMRLSLADAGDGLEDANFVYAMADAAILRLFT 781
Query: 801 EIAWMEDILQADSSSFLRTGPPSTYADRVFENEINIAVKTTEQNYKDYMFREALKSGFYD 860
I W++++++ + LR +ADRVF NE+N + TEQNY+ F++ALK+GF++
Sbjct: 782 MIEWIKEMIEQRDAGLLRK-DAKKFADRVFANEMNSLIAATEQNYEATNFKDALKTGFFE 841
Query: 861 LQAARDEYRFSCGA--GGMNRDLVFRFMDVQTRLITPICPHYAEYVWREMLKKEGFVVNA 920
QA RD YR C M+ LVFRF++ Q +++PICPH AEY+W ++L K+G +VNA
Sbjct: 842 YQAIRDTYRELCAGIDEPMSESLVFRFIETQMLILSPICPHIAEYIW-QLLGKDGLIVNA 901
Query: 921 GWPSAGSPDLTLKSANKYLQDSIVLMRKLLQKQLLGSKKGNKKGAPVTSVIEDKKLTGLI 980
WP+ D L +++ +S+ R L+ + KK K+ I + +I
Sbjct: 902 PWPTVDPVDEKLAIGARFITESLAEFRARLKTYMTPKKKALKE-------IPEVPTEAVI 961
Query: 981 YVNEQFDGWKAECLRILRCKFDSTKRTFAPDSEIMEALQKSSVGQASDFRQTQKLCMPFL 1040
YV +++ W+ L IL K PD++ + L +G+ ++ K MPF+
Sbjct: 962 YVAKEYPPWQKTILDILE-KQAKANNGALPDNKAISQL----IGKEESLKKFAKKAMPFV 1021
Query: 1041 RFKKDEVAALGVHALDLKLPFGEIEVLKENLELIKRQIGLEEVEV 1073
+ K+ GV AL P + +L EN++ I + L+ V +
Sbjct: 1022 QMIKERFEQKGVSALASSSPVDQTSILNENIDFIMNALDLDRVTI 1042
BLAST of Carg12321 vs. TrEMBL
Match:
tr|A0A1S3CKD2|A0A1S3CKD2_CUCME (leucine--tRNA ligase, cytoplasmic OS=Cucumis melo OX=3656 GN=LOC103501922 PE=3 SV=1)
HSP 1 Score: 2104.3 bits (5451), Expect = 0.0e+00
Identity = 1014/1090 (93.03%), Postives = 1063/1090 (97.52%), Query Frame = 0
Query: 19 MASECGKSFARRDSLREIESKVRVLWEENDVFRAEACETPPKEGEKFFGNFPFPYMNGFL 78
MASE GKSFARRDSLREIE+KVRVLWEENDVFRAEACETPPK GEKFFGNFPFPYMNGFL
Sbjct: 1 MASESGKSFARRDSLREIEAKVRVLWEENDVFRAEACETPPKVGEKFFGNFPFPYMNGFL 60
Query: 79 HLGHAFSLSKLEFAAAYHRLRGANVLLPFGFHCTGMPIKASADKLAREIQQFGDPPVFPR 138
H+GHAFSLSKLEFAAAYHRLRGANVLLPFGFHCTGMPIKASADKLAREIQQFGDPPVFPR
Sbjct: 61 HIGHAFSLSKLEFAAAYHRLRGANVLLPFGFHCTGMPIKASADKLAREIQQFGDPPVFPR 120
Query: 139 QMEEQENLKAESEDANESNPTLPDKFKGKKSKAVSKSSGQMYQWEIMRSFGLSDSEISKF 198
+EEQ+NLKAE+E+ NESNP LPDKFKGKKSKA SK+ GQMYQWEIMRSFGLSDSEISKF
Sbjct: 121 DIEEQQNLKAEAEEGNESNPMLPDKFKGKKSKAASKTGGQMYQWEIMRSFGLSDSEISKF 180
Query: 199 QNPYNWLTFFPPLAMEDLKAFGLGCDWRRSFITTDMNPFFDSFVQWQMRKLKSMGKIVKD 258
Q+PYNWLTFFPPLAMEDLKAFGLGCDWRRSFITTDMNP++DSF+QWQMRKLKSMGKIVKD
Sbjct: 181 QDPYNWLTFFPPLAMEDLKAFGLGCDWRRSFITTDMNPYYDSFIQWQMRKLKSMGKIVKD 240
Query: 259 VRYTIYSPLDGQPCADHDRASGEGVQPQDYTLIKMEVVPPFPPKMGVLEGRKVYLAAATL 318
VRYTIYSPLDGQPCADHDRA+GEGVQPQDYTLIKMEVV PFPPK+GVLEGRKV+LAAATL
Sbjct: 241 VRYTIYSPLDGQPCADHDRATGEGVQPQDYTLIKMEVVAPFPPKLGVLEGRKVFLAAATL 300
Query: 319 RPETMYGQTNAWVLPEGKYGAFEINDTDVFIITERAALNLAYQKFSRVPEKPTCLIQLTG 378
RPETMYGQTNAWVLP+GKYGAFEINDTDVFIITERAALNLAYQ+FS+VPEKPTCLIQLTG
Sbjct: 301 RPETMYGQTNAWVLPDGKYGAFEINDTDVFIITERAALNLAYQRFSKVPEKPTCLIQLTG 360
Query: 379 NDLIGLPLKSPLAFNEIIYALPMLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRA 438
NDLIGLPLKSPLAFNEIIYALPMLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRA
Sbjct: 361 NDLIGLPLKSPLAFNEIIYALPMLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRA 420
Query: 439 KYGVKDEWVLPYDIVPIIDIPEFGDRAAEKVCVDLKIKSQNEKDKLAEAKRLTYLKGFTD 498
KYGVKDEWVLPYDIVPIIDIPEFGDRAAEKVC+DLKIKSQNEKDKLAEAKRLTYL+GFTD
Sbjct: 421 KYGVKDEWVLPYDIVPIIDIPEFGDRAAEKVCLDLKIKSQNEKDKLAEAKRLTYLRGFTD 480
Query: 499 GTLIVGEFSGRKVQEAKPMIRSKLIETGQAIPYSEPEKRVTSRSGDECIVALTDQWYITY 558
GTL+VGEF+GRKVQEAKP+IRS+LIETGQAIPYSEPEKRV SRSGDECIVALTDQWYITY
Sbjct: 481 GTLVVGEFAGRKVQEAKPLIRSQLIETGQAIPYSEPEKRVMSRSGDECIVALTDQWYITY 540
Query: 559 GESEWKKLAEDCLANMNLFSDETRHGFQHTLSWLNQWACSRSFGLGTRIPWDEQFLVESL 618
GESEWKKL+E+CLA+M++FSDET+HGF+HTL WLNQWACSRSFGLGTRIPWD+QFLVESL
Sbjct: 541 GESEWKKLSEECLASMDMFSDETKHGFEHTLGWLNQWACSRSFGLGTRIPWDKQFLVESL 600
Query: 619 SDSTIYMAYYTIAHLLHNGDLYGSGASAIKPEQMTDEVWDFVFCGAAEPKSTGISRSILN 678
SDSTIYMAYYTIAHLL NGDLYGSG SA+KPEQMTDEVWDFVFCGAAEPKSTGIS+SILN
Sbjct: 601 SDSTIYMAYYTIAHLLQNGDLYGSGDSAVKPEQMTDEVWDFVFCGAAEPKSTGISQSILN 660
Query: 679 KMKQEFEYWYPFDLRVSGKDLIQNHLTFCIYNHTAIMPEHHWPRGFRCNGHIMLNSEKMS 738
KMKQEFEYWYPFDLRVSGKDLIQNHLTF IYNHTAIMP+ HWPR FRCNGHIMLNSEKMS
Sbjct: 661 KMKQEFEYWYPFDLRVSGKDLIQNHLTFTIYNHTAIMPKRHWPRAFRCNGHIMLNSEKMS 720
Query: 739 KSTGNFRTLRQAIEEFSADATRFSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDIL 798
KSTGNFRTLR+AIEEFSADATRFSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDIL
Sbjct: 721 KSTGNFRTLREAIEEFSADATRFSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDIL 780
Query: 799 QADSSSFLRTGPPSTYADRVFENEINIAVKTTEQNYKDYMFREALKSGFYDLQAARDEYR 858
QADSSSFLRTGPPSTYADRVFENEINIAVK TEQNYKDYMFREALK+GFYDLQAARDEYR
Sbjct: 781 QADSSSFLRTGPPSTYADRVFENEINIAVKMTEQNYKDYMFREALKTGFYDLQAARDEYR 840
Query: 859 FSCGAGGMNRDLVFRFMDVQTRLITPICPHYAEYVWREMLKKEGFVVNAGWPSAGSPDLT 918
FSCGAGGMNRDLVFRFMDVQTRLITPICPHYAE+VWR MLKK+GFVVNAGWPSA SPDLT
Sbjct: 841 FSCGAGGMNRDLVFRFMDVQTRLITPICPHYAEHVWRNMLKKDGFVVNAGWPSADSPDLT 900
Query: 919 LKSANKYLQDSIVLMRKLLQKQLLGSKKGNKKGAPVTSVIEDKKLTGLIYVNEQFDGWKA 978
LKSANKYLQDSIVLMRKLLQKQLLGSKKGNKKG PVT+VIEDKKLTGLIYVNEQFDGWKA
Sbjct: 901 LKSANKYLQDSIVLMRKLLQKQLLGSKKGNKKGTPVTTVIEDKKLTGLIYVNEQFDGWKA 960
Query: 979 ECLRILRCKFDSTKRTFAPDSEIMEALQKSSVGQASDFRQTQKLCMPFLRFKKDEVAALG 1038
ECLRIL+ KFDSTKRTFAPDSEIMEALQKSSVGQA+DFRQTQKLCMPFLRFKKDE +LG
Sbjct: 961 ECLRILQSKFDSTKRTFAPDSEIMEALQKSSVGQAADFRQTQKLCMPFLRFKKDEAVSLG 1020
Query: 1039 VHALDLKLPFGEIEVLKENLELIKRQIGLEEVEVLCASDTDAIAKAGALASLLKQNPPSP 1098
V ALDL+LPFGE++VL ENLELI+RQIGLEEV+VL SD +A+AKAGALASLLKQNPPSP
Sbjct: 1021 VQALDLRLPFGEMDVLNENLELIRRQIGLEEVQVLRVSDPNALAKAGALASLLKQNPPSP 1080
Query: 1099 GNPTAIFLTS 1109
GNPTAIFLTS
Sbjct: 1081 GNPTAIFLTS 1090
BLAST of Carg12321 vs. TrEMBL
Match:
tr|A0A0A0LV91|A0A0A0LV91_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G084310 PE=3 SV=1)
HSP 1 Score: 2100.5 bits (5441), Expect = 0.0e+00
Identity = 1013/1090 (92.94%), Postives = 1061/1090 (97.34%), Query Frame = 0
Query: 19 MASECGKSFARRDSLREIESKVRVLWEENDVFRAEACETPPKEGEKFFGNFPFPYMNGFL 78
MASE GKSFARRDSLREIE+K+RVLWEENDVFRAEACE PPK GEKFFGNFPFPYMNGFL
Sbjct: 1 MASESGKSFARRDSLREIEAKIRVLWEENDVFRAEACEVPPKVGEKFFGNFPFPYMNGFL 60
Query: 79 HLGHAFSLSKLEFAAAYHRLRGANVLLPFGFHCTGMPIKASADKLAREIQQFGDPPVFPR 138
H+GHAFSLSKLEFAAAYHRLRGANVLLPFGFHCTGMPIKASADKLAREIQQFGDPPVFPR
Sbjct: 61 HIGHAFSLSKLEFAAAYHRLRGANVLLPFGFHCTGMPIKASADKLAREIQQFGDPPVFPR 120
Query: 139 QMEEQENLKAESEDANESNPTLPDKFKGKKSKAVSKSSGQMYQWEIMRSFGLSDSEISKF 198
+ EEQ+NLKAE+EDANESNPTLPDKFKGKKSKA SK+ QMYQWEIMRSFGLSDSEISKF
Sbjct: 121 ETEEQQNLKAEAEDANESNPTLPDKFKGKKSKAASKTGVQMYQWEIMRSFGLSDSEISKF 180
Query: 199 QNPYNWLTFFPPLAMEDLKAFGLGCDWRRSFITTDMNPFFDSFVQWQMRKLKSMGKIVKD 258
Q+PYNWLTFFPP AMEDLKAFGLGCDWRRSFITTD+NP++DSF+QWQMRKLKSMGKIVKD
Sbjct: 181 QDPYNWLTFFPPFAMEDLKAFGLGCDWRRSFITTDVNPYYDSFIQWQMRKLKSMGKIVKD 240
Query: 259 VRYTIYSPLDGQPCADHDRASGEGVQPQDYTLIKMEVVPPFPPKMGVLEGRKVYLAAATL 318
VRYTIYSPLDGQPCADHDRASGEGVQPQDYTLIKMEVV PFPPK+GVLEGRKV+LAAATL
Sbjct: 241 VRYTIYSPLDGQPCADHDRASGEGVQPQDYTLIKMEVVAPFPPKLGVLEGRKVFLAAATL 300
Query: 319 RPETMYGQTNAWVLPEGKYGAFEINDTDVFIITERAALNLAYQKFSRVPEKPTCLIQLTG 378
RPETMYGQTNAWVLPEGKYGAFEINDTDVFIITERAALNLAYQ+FS+VPEKPTCLIQLTG
Sbjct: 301 RPETMYGQTNAWVLPEGKYGAFEINDTDVFIITERAALNLAYQRFSKVPEKPTCLIQLTG 360
Query: 379 NDLIGLPLKSPLAFNEIIYALPMLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRA 438
NDLIGLPLKSPLAFNEIIYALPMLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRA
Sbjct: 361 NDLIGLPLKSPLAFNEIIYALPMLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRA 420
Query: 439 KYGVKDEWVLPYDIVPIIDIPEFGDRAAEKVCVDLKIKSQNEKDKLAEAKRLTYLKGFTD 498
KYGVKDEWVLPYDIVPIIDIPEFGDRAAEKVC+DLKIKSQNEKDKLAEAKRLTYL+GFTD
Sbjct: 421 KYGVKDEWVLPYDIVPIIDIPEFGDRAAEKVCLDLKIKSQNEKDKLAEAKRLTYLRGFTD 480
Query: 499 GTLIVGEFSGRKVQEAKPMIRSKLIETGQAIPYSEPEKRVTSRSGDECIVALTDQWYITY 558
GTLIVGEF+GRKVQEAKP+IRS+LIETGQAIPYSEPEKRV SRSGDECIVALTDQWYI Y
Sbjct: 481 GTLIVGEFAGRKVQEAKPLIRSQLIETGQAIPYSEPEKRVMSRSGDECIVALTDQWYIIY 540
Query: 559 GESEWKKLAEDCLANMNLFSDETRHGFQHTLSWLNQWACSRSFGLGTRIPWDEQFLVESL 618
GESEWKKL+E+CLA+M++FSDETRHGF+HTL WLNQWACSRSFGLGTRIPWD+QFLVESL
Sbjct: 541 GESEWKKLSEECLASMDMFSDETRHGFEHTLGWLNQWACSRSFGLGTRIPWDKQFLVESL 600
Query: 619 SDSTIYMAYYTIAHLLHNGDLYGSGASAIKPEQMTDEVWDFVFCGAAEPKSTGISRSILN 678
SDSTIYMAYYTIAHLL NGDLYGSG SA+KPEQMTDEVWDFVFCGAAEPKSTGIS+SILN
Sbjct: 601 SDSTIYMAYYTIAHLLQNGDLYGSGDSAVKPEQMTDEVWDFVFCGAAEPKSTGISQSILN 660
Query: 679 KMKQEFEYWYPFDLRVSGKDLIQNHLTFCIYNHTAIMPEHHWPRGFRCNGHIMLNSEKMS 738
KMKQEFEYWYPFDLRVSGKDLIQNHLTF IYNHTAIMP+ HWPR FRCNGHIMLNSEKMS
Sbjct: 661 KMKQEFEYWYPFDLRVSGKDLIQNHLTFTIYNHTAIMPKRHWPRAFRCNGHIMLNSEKMS 720
Query: 739 KSTGNFRTLRQAIEEFSADATRFSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDIL 798
KSTGNFRTLR+AIEEFSADATRFSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDIL
Sbjct: 721 KSTGNFRTLREAIEEFSADATRFSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDIL 780
Query: 799 QADSSSFLRTGPPSTYADRVFENEINIAVKTTEQNYKDYMFREALKSGFYDLQAARDEYR 858
QADSSSFLRTGPPSTYADRVFENEINIAVK TEQNYKDYMFREALK+GFYDLQAARDEYR
Sbjct: 781 QADSSSFLRTGPPSTYADRVFENEINIAVKMTEQNYKDYMFREALKTGFYDLQAARDEYR 840
Query: 859 FSCGAGGMNRDLVFRFMDVQTRLITPICPHYAEYVWREMLKKEGFVVNAGWPSAGSPDLT 918
FSCGAGGMNRDLVFRFMDVQTRLITPICPHYAE+VWR MLKK GFVVNAGWPSA SPDLT
Sbjct: 841 FSCGAGGMNRDLVFRFMDVQTRLITPICPHYAEHVWRNMLKKNGFVVNAGWPSADSPDLT 900
Query: 919 LKSANKYLQDSIVLMRKLLQKQLLGSKKGNKKGAPVTSVIEDKKLTGLIYVNEQFDGWKA 978
LKSANKYLQDSIVLMRKLLQKQLLGSKKGNKKGAPVT+V+EDKKLTGLIYVNEQFDGWKA
Sbjct: 901 LKSANKYLQDSIVLMRKLLQKQLLGSKKGNKKGAPVTTVVEDKKLTGLIYVNEQFDGWKA 960
Query: 979 ECLRILRCKFDSTKRTFAPDSEIMEALQKSSVGQASDFRQTQKLCMPFLRFKKDEVAALG 1038
ECLRIL+ KFDSTKRTFAPDSEIMEALQKSSVGQA+DFRQTQKLCMPFLRFKKDE +LG
Sbjct: 961 ECLRILQSKFDSTKRTFAPDSEIMEALQKSSVGQAADFRQTQKLCMPFLRFKKDEAVSLG 1020
Query: 1039 VHALDLKLPFGEIEVLKENLELIKRQIGLEEVEVLCASDTDAIAKAGALASLLKQNPPSP 1098
V AL+L+LPFGE++VL ENLELI+RQIGLEEV++L SD +A+AKAGALASLLKQNPPSP
Sbjct: 1021 VQALNLRLPFGEMDVLNENLELIRRQIGLEEVQILRVSDPNALAKAGALASLLKQNPPSP 1080
Query: 1099 GNPTAIFLTS 1109
GNPTAIFLTS
Sbjct: 1081 GNPTAIFLTS 1090
BLAST of Carg12321 vs. TrEMBL
Match:
tr|A0A2P4IKW3|A0A2P4IKW3_QUESU (Leucine--trna ligase, cytoplasmic OS=Quercus suber OX=58331 GN=CFP56_66340 PE=4 SV=1)
HSP 1 Score: 1917.1 bits (4965), Expect = 0.0e+00
Identity = 913/1090 (83.76%), Postives = 1006/1090 (92.29%), Query Frame = 0
Query: 18 DMASECGKSFARRDSLREIESKVRVLWEENDVFRAEACETPPKEGEKFFGNFPFPYMNGF 77
DMA E GKSFARRD L +IE VR W+E DVFRAE+CE PP+ GEKFFGNFP+PYMNG+
Sbjct: 592 DMAGEGGKSFARRDRLLQIEHDVRKWWDEEDVFRAESCEKPPEPGEKFFGNFPYPYMNGY 651
Query: 78 LHLGHAFSLSKLEFAAAYHRLRGANVLLPFGFHCTGMPIKASADKLAREIQQFGDPPVFP 137
LHLGHAFSLSKLEFAAAYHRLRGANVL PF FHCTGMPIKASADKLAREIQQFGDPP FP
Sbjct: 652 LHLGHAFSLSKLEFAAAYHRLRGANVLCPFAFHCTGMPIKASADKLAREIQQFGDPPNFP 711
Query: 138 RQMEEQENLKAESEDANESNPTLPDKFKGKKSKAVSKSSGQMYQWEIMRSFGLSDSEISK 197
+ EE+ + E+E+AN P PDKFKGKKSKA SKS GQ +QWEIMRSFGLSD+EISK
Sbjct: 712 HESEEEIDTVQEAEEANPGAP--PDKFKGKKSKAASKSGGQKFQWEIMRSFGLSDNEISK 771
Query: 198 FQNPYNWLTFFPPLAMEDLKAFGLGCDWRRSFITTDMNPFFDSFVQWQMRKLKSMGKIVK 257
FQNPYNWLT+FPPLA EDL AFGLGCDWRRSFITTDMNPF+DSFV+WQMRKLKSMGKIVK
Sbjct: 772 FQNPYNWLTYFPPLAKEDLIAFGLGCDWRRSFITTDMNPFYDSFVRWQMRKLKSMGKIVK 831
Query: 258 DVRYTIYSPLDGQPCADHDRASGEGVQPQDYTLIKMEVVPPFPPKMGVLEGRKVYLAAAT 317
DVRYTI+SPLDGQPCADHDRASGEGVQPQ+YT+IKME+V PFPPK+GVLEGR+V+LAAAT
Sbjct: 832 DVRYTIFSPLDGQPCADHDRASGEGVQPQEYTIIKMELVSPFPPKLGVLEGRRVFLAAAT 891
Query: 318 LRPETMYGQTNAWVLPEGKYGAFEINDTDVFIITERAALNLAYQKFSRVPEKPTCLIQLT 377
LRPETMYGQTNAWVLP+GKYGA+EINDTDVFI+T+RAALNLAYQK+SR+PEKPTCL++LT
Sbjct: 892 LRPETMYGQTNAWVLPDGKYGAYEINDTDVFILTQRAALNLAYQKYSRIPEKPTCLVELT 951
Query: 378 GNDLIGLPLKSPLAFNEIIYALPMLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALR 437
G DLIGLPL+SPL+FNEIIYALPMLTILTDKGTGIVTSVPSDAPDDYMA+HDLKSKPALR
Sbjct: 952 GYDLIGLPLRSPLSFNEIIYALPMLTILTDKGTGIVTSVPSDAPDDYMALHDLKSKPALR 1011
Query: 438 AKYGVKDEWVLPYDIVPIIDIPEFGDRAAEKVCVDLKIKSQNEKDKLAEAKRLTYLKGFT 497
AKYGVKDEWVLP++I+PII+IPEFGDRAAEKVC+DLKIKSQNEK+KLA+AK+LTYLKGFT
Sbjct: 1012 AKYGVKDEWVLPFEIIPIINIPEFGDRAAEKVCMDLKIKSQNEKEKLADAKKLTYLKGFT 1071
Query: 498 DGTLIVGEFSGRKVQEAKPMIRSKLIETGQAIPYSEPEKRVTSRSGDECIVALTDQWYIT 557
+GT++VGEF+GRKVQE K +IRSKL+E G AI YSEPEKRV SRSGD+CIVALTDQWY+T
Sbjct: 1072 EGTMLVGEFTGRKVQETKALIRSKLLEMGLAIMYSEPEKRVVSRSGDDCIVALTDQWYLT 1131
Query: 558 YGESEWKKLAEDCLANMNLFSDETRHGFQHTLSWLNQWACSRSFGLGTRIPWDEQFLVES 617
YGE EW+KLAE+CL+NMNL+S+ETRHGF+HTLSWLNQWACSRSFGLGTRIPWDEQFLVES
Sbjct: 1132 YGEEEWRKLAEECLSNMNLYSEETRHGFEHTLSWLNQWACSRSFGLGTRIPWDEQFLVES 1191
Query: 618 LSDSTIYMAYYTIAHLLHNGDLYGSGASAIKPEQMTDEVWDFVFCGAAEPKSTGISRSIL 677
LSDSTIYMAYYT+AHLL NGD+YGS S+IKPEQ+TDEVWDF+FCG PKS+ IS SIL
Sbjct: 1192 LSDSTIYMAYYTVAHLLQNGDIYGSSRSSIKPEQLTDEVWDFIFCGGTYPKSSDISSSIL 1251
Query: 678 NKMKQEFEYWYPFDLRVSGKDLIQNHLTFCIYNHTAIMPEHHWPRGFRCNGHIMLNSEKM 737
NKMKQEFEYWYPFDLRVSGKDLIQNHLTF IYNHTAIM + HWPRGFRCNGHIMLNSEKM
Sbjct: 1252 NKMKQEFEYWYPFDLRVSGKDLIQNHLTFTIYNHTAIMSKKHWPRGFRCNGHIMLNSEKM 1311
Query: 738 SKSTGNFRTLRQAIEEFSADATRFSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDI 797
SKSTGNFRTLRQAIEEFSADATRFSLADAGDGVDDANFVFETANAAILRLTKEI+WME++
Sbjct: 1312 SKSTGNFRTLRQAIEEFSADATRFSLADAGDGVDDANFVFETANAAILRLTKEISWMEEV 1371
Query: 798 LQADSSSFLRTGPPSTYADRVFENEINIAVKTTEQNYKDYMFREALKSGFYDLQAARDEY 857
L A+SS LRTGPPSTYADRVF N+INIAVK TEQNY+DYMFREALK+GFYDLQAARDEY
Sbjct: 1372 LAAESS--LRTGPPSTYADRVFANDINIAVKMTEQNYQDYMFREALKTGFYDLQAARDEY 1431
Query: 858 RFSCGAGGMNRDLVFRFMDVQTRLITPICPHYAEYVWREMLKKEGFVVNAGWPSAGSPDL 917
RFSCGAGGMNRDLV+ FMDVQTRLI PICPHY EYVWR +L K GFVV AGWP A SPDL
Sbjct: 1432 RFSCGAGGMNRDLVWHFMDVQTRLIAPICPHYGEYVWRILLNKNGFVVKAGWPVADSPDL 1491
Query: 918 TLKSANKYLQDSIVLMRKLLQKQLLGSKKGNKKGAPVTSVIEDKKLTGLIYVNEQFDGWK 977
TLK ANKYLQDSIVLMRKLLQKQ+LGSKK NKKGAPVT++ ED +L GLI+VNEQFDGWK
Sbjct: 1492 TLKIANKYLQDSIVLMRKLLQKQILGSKKANKKGAPVTTLSEDNRLAGLIFVNEQFDGWK 1551
Query: 978 AECLRILRCKFDSTKRTFAPDSEIMEALQKSSVGQASDFRQTQKLCMPFLRFKKDEVAAL 1037
AECLRIL+ KFDS RTFAPD EIM+AL+ SS+GQ ++F+QTQKLCMPFLRFKKDE AL
Sbjct: 1552 AECLRILQSKFDSKTRTFAPDGEIMDALKNSSIGQTANFKQTQKLCMPFLRFKKDEAVAL 1611
Query: 1038 GVHALDLKLPFGEIEVLKENLELIKRQIGLEEVEVLCASDTDAIAKAGALASLLKQNPPS 1097
G AL+L+LPFGEIEVL+ENL+LI+RQIGLEEVE+L A+D +A+AKAG L SLL QNPPS
Sbjct: 1612 GAQALELRLPFGEIEVLQENLDLIRRQIGLEEVEILSATDPNALAKAGTLVSLLNQNPPS 1671
Query: 1098 PGNPTAIFLT 1108
PGNPTAIFLT
Sbjct: 1672 PGNPTAIFLT 1677
BLAST of Carg12321 vs. TrEMBL
Match:
tr|A0A067LEE1|A0A067LEE1_JATCU (Uncharacterized protein OS=Jatropha curcas OX=180498 GN=JCGZ_00261 PE=3 SV=1)
HSP 1 Score: 1905.2 bits (4934), Expect = 0.0e+00
Identity = 918/1090 (84.22%), Postives = 1003/1090 (92.02%), Query Frame = 0
Query: 19 MASECGKSFARRDSLREIESKVRVLWEENDVFRAEACETPPKEGEKFFGNFPFPYMNGFL 78
MASE KSFARRD L EIE KVR WE+ DVFRAE E PP+ EKFFGNFPFPYMNGFL
Sbjct: 1 MASEGAKSFARRDRLLEIEQKVRGWWEDKDVFRAEPGERPPEADEKFFGNFPFPYMNGFL 60
Query: 79 HLGHAFSLSKLEFAAAYHRLRGANVLLPFGFHCTGMPIKASADKLAREIQQFGDPPVFPR 138
HLGHAFSLSKLEFAAAYHRLRGANVLLPF FHCTGMPIKASADKL REI++FG+PP F
Sbjct: 61 HLGHAFSLSKLEFAAAYHRLRGANVLLPFAFHCTGMPIKASADKLRREIERFGNPPNFAI 120
Query: 139 QMEEQENLKAESEDANESNPTLPDKFKGKKSKAVSKSSGQMYQWEIMRSFGLSDSEISKF 198
+ EEQ + E +DA S P DKFKGKKSKA SKS GQM+QWEIMRSFGLSDSEISKF
Sbjct: 121 EEEEQVETQTEPDDALGSLPV--DKFKGKKSKAASKSGGQMFQWEIMRSFGLSDSEISKF 180
Query: 199 QNPYNWLTFFPPLAMEDLKAFGLGCDWRRSFITTDMNPFFDSFVQWQMRKLKSMGKIVKD 258
Q+PY WL FFPPLAMEDLKAFGLGCDWRRSF+TTD+NP+FDSFVQWQMRKLKSMGKIVKD
Sbjct: 181 QDPYEWLRFFPPLAMEDLKAFGLGCDWRRSFVTTDINPYFDSFVQWQMRKLKSMGKIVKD 240
Query: 259 VRYTIYSPLDGQPCADHDRASGEGVQPQDYTLIKMEVVPPFPPKMGVLEGRKVYLAAATL 318
VR+TIYSPLDGQPCADHDRASGEGVQPQ+YTLIKMEV+PPF K+G LEG+KV+LAAATL
Sbjct: 241 VRFTIYSPLDGQPCADHDRASGEGVQPQEYTLIKMEVLPPFTVKLGPLEGKKVFLAAATL 300
Query: 319 RPETMYGQTNAWVLPEGKYGAFEINDTDVFIITERAALNLAYQKFSRVPEKPTCLIQLTG 378
RPETMYGQTNAWVLP+GKYGAFEIN+TDV I+TERAALNLAYQ SR P+KP+CL++LTG
Sbjct: 301 RPETMYGQTNAWVLPDGKYGAFEINETDVLILTERAALNLAYQNVSRFPQKPSCLLELTG 360
Query: 379 NDLIGLPLKSPLAFNEIIYALPMLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRA 438
DLIGL LKSPL+FNE+IYALPMLTILTDKGTGIVTSVPSDAPDDYMA+HDLK+KPA RA
Sbjct: 361 YDLIGLRLKSPLSFNEVIYALPMLTILTDKGTGIVTSVPSDAPDDYMALHDLKAKPAFRA 420
Query: 439 KYGVKDEWVLPYDIVPIIDIPEFGDRAAEKVCVDLKIKSQNEKDKLAEAKRLTYLKGFTD 498
KYGVKDEWVLP++IVPII+IP+FGD+AAEKVC+DLKIKSQNEKDKLAEAKRLTYL+GFTD
Sbjct: 421 KYGVKDEWVLPFEIVPIINIPDFGDKAAEKVCLDLKIKSQNEKDKLAEAKRLTYLRGFTD 480
Query: 499 GTLIVGEFSGRKVQEAKPMIRSKLIETGQAIPYSEPEKRVTSRSGDECIVALTDQWYITY 558
GT++VGEF+GRKVQEAKP+IR+KLIETG+AI YSEPEKRV SRSGDEC+VALTDQWYITY
Sbjct: 481 GTMLVGEFAGRKVQEAKPLIRAKLIETGEAIMYSEPEKRVVSRSGDECVVALTDQWYITY 540
Query: 559 GESEWKKLAEDCLANMNLFSDETRHGFQHTLSWLNQWACSRSFGLGTRIPWDEQFLVESL 618
GE EWKKLAE+C +NMNL+SDETRHGF+HTLSWLNQWACSRSFGLGTRIPWD++FLVESL
Sbjct: 541 GEEEWKKLAEECFSNMNLYSDETRHGFEHTLSWLNQWACSRSFGLGTRIPWDKEFLVESL 600
Query: 619 SDSTIYMAYYTIAHLLHNGDLYGSG-ASAIKPEQMTDEVWDFVFCGAAEPKSTGISRSIL 678
SDSTIYMAYYT+AHLLHN D+YG+ ++KPEQMTDEVWDF+ CG PKS+ I SIL
Sbjct: 601 SDSTIYMAYYTVAHLLHNDDMYGTNKPHSVKPEQMTDEVWDFIICGGPHPKSSDIPSSIL 660
Query: 679 NKMKQEFEYWYPFDLRVSGKDLIQNHLTFCIYNHTAIMPEHHWPRGFRCNGHIMLNSEKM 738
+KMKQEFEYWYPFDLRVSGKDLIQNHLTFCIYNHTAIM +HHWPRGFRCNGH+MLNSEKM
Sbjct: 661 DKMKQEFEYWYPFDLRVSGKDLIQNHLTFCIYNHTAIMAKHHWPRGFRCNGHMMLNSEKM 720
Query: 739 SKSTGNFRTLRQAIEEFSADATRFSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDI 798
SKSTGNFRT+RQAIEEFSADATRFSLADAGDGVDDANFVFETANAAILRLTKEIAWME++
Sbjct: 721 SKSTGNFRTVRQAIEEFSADATRFSLADAGDGVDDANFVFETANAAILRLTKEIAWMEEV 780
Query: 799 LQADSSSFLRTGPPSTYADRVFENEINIAVKTTEQNYKDYMFREALKSGFYDLQAARDEY 858
L A+SS LR GPPSTYADRVFENEINIAV+ TEQNY+ YMFREALKSGFYDLQAARDEY
Sbjct: 781 LAAESS--LRIGPPSTYADRVFENEINIAVRMTEQNYRGYMFREALKSGFYDLQAARDEY 840
Query: 859 RFSCGAGGMNRDLVFRFMDVQTRLITPICPHYAEYVWREMLKKEGFVVNAGWPSAGSPDL 918
RFSCG+GGMNRDLV+RFMDVQTRLITPICPHYAEYVWRE+LKK+GFVV AGWP AGSPDL
Sbjct: 841 RFSCGSGGMNRDLVWRFMDVQTRLITPICPHYAEYVWRELLKKDGFVVKAGWPMAGSPDL 900
Query: 919 TLKSANKYLQDSIVLMRKLLQKQLLGSKKGNKKGAPVTSVIEDKKLTGLIYVNEQFDGWK 978
TLK+ANKYLQDSIVLMRKLLQKQLLGSKKGNKKGAPV ++ ED KLTGLIYVNEQFDGWK
Sbjct: 901 TLKAANKYLQDSIVLMRKLLQKQLLGSKKGNKKGAPVATLTED-KLTGLIYVNEQFDGWK 960
Query: 979 AECLRILRCKFDSTKRTFAPDSEIMEALQKSSVGQASDFRQTQKLCMPFLRFKKDEVAAL 1038
AECLRIL+ KFD RT APD EIM+AL+ SSVGQA++F+QTQKLCMPFLRFKKDE A+
Sbjct: 961 AECLRILQSKFDHKNRTVAPDGEIMDALKNSSVGQATNFKQTQKLCMPFLRFKKDEAIAI 1020
Query: 1039 GVHALDLKLPFGEIEVLKENLELIKRQIGLEEVEVLCASDTDAIAKAGALASLLKQNPPS 1098
G ALDLKLPFGEIEVLKENL+LIKRQIGLEEVE+L A+D DA+AKAG+L SLL QNPPS
Sbjct: 1021 GSQALDLKLPFGEIEVLKENLDLIKRQIGLEEVEILSAADPDALAKAGSLVSLLNQNPPS 1080
Query: 1099 PGNPTAIFLT 1108
PGNPTAIFLT
Sbjct: 1081 PGNPTAIFLT 1085
BLAST of Carg12321 vs. TrEMBL
Match:
tr|A0A1U8MZX1|A0A1U8MZX1_GOSHI (leucine--tRNA ligase, cytoplasmic-like OS=Gossypium hirsutum OX=3635 GN=LOC107941996 PE=3 SV=1)
HSP 1 Score: 1904.0 bits (4931), Expect = 0.0e+00
Identity = 908/1088 (83.46%), Postives = 1001/1088 (92.00%), Query Frame = 0
Query: 19 MASECGKSFARRDSLREIESKVRVLWEENDVFRAEACETPPKEGEKFFGNFPFPYMNGFL 78
MA+E GKSFARRD L EIESKVRV W+E DVF+AE E PP+ GEKFFGNFPFPYMNGFL
Sbjct: 1 MATEGGKSFARRDKLLEIESKVRVWWDEKDVFKAEPAEKPPQPGEKFFGNFPFPYMNGFL 60
Query: 79 HLGHAFSLSKLEFAAAYHRLRGANVLLPFGFHCTGMPIKASADKLAREIQQFGDPPVFPR 138
HLGHAFSLSKLEFAAAYHRLRGANVLLPF FHCTGMPIKASADKLAREIQQFG+PP FP
Sbjct: 61 HLGHAFSLSKLEFAAAYHRLRGANVLLPFAFHCTGMPIKASADKLAREIQQFGNPPAFPH 120
Query: 139 QMEEQENLKAESEDANESNPTLPDKFKGKKSKAVSKSSGQMYQWEIMRSFGLSDSEISKF 198
++ E+E E D NE +PDKFKGKKSKA SKSSGQ++QWEIMRSFGLSDSEISKF
Sbjct: 121 EVVEEEPNPKEESDRNEGANVVPDKFKGKKSKAASKSSGQIFQWEIMRSFGLSDSEISKF 180
Query: 199 QNPYNWLTFFPPLAMEDLKAFGLGCDWRRSFITTDMNPFFDSFVQWQMRKLKSMGKIVKD 258
QNPY WL FFPPLA+EDLKAFGLGCDWRRSF+TTDMNPFFDSFV+WQMRKLKS+GKIVKD
Sbjct: 181 QNPYEWLKFFPPLAVEDLKAFGLGCDWRRSFVTTDMNPFFDSFVKWQMRKLKSLGKIVKD 240
Query: 259 VRYTIYSPLDGQPCADHDRASGEGVQPQDYTLIKMEVVPPFPPKMGVLEGRKVYLAAATL 318
+RYTIYSPLDGQPCADHDRA+GEGVQPQ+YT+IKMEVV PFP KM VLEG+KV+LAAATL
Sbjct: 241 MRYTIYSPLDGQPCADHDRATGEGVQPQEYTIIKMEVVSPFPAKMRVLEGKKVFLAAATL 300
Query: 319 RPETMYGQTNAWVLPEGKYGAFEINDTDVFIITERAALNLAYQKFSRVPEKPTCLIQLTG 378
RPETMYGQTN WVLP+GKYGAFEINDTDVFI+TERAALNLAYQK S VPEKP+CL++LTG
Sbjct: 301 RPETMYGQTNCWVLPDGKYGAFEINDTDVFILTERAALNLAYQKLSLVPEKPSCLVELTG 360
Query: 379 NDLIGLPLKSPLAFNEIIYALPMLTILTDKGTGIVTSVPSDAPDDYMAMHDLKSKPALRA 438
DLIGLP+K PL+FNEIIYALPMLTILTDKGTGIVTSVPSDAPDDYMA+ DLK+KPA RA
Sbjct: 361 YDLIGLPVKPPLSFNEIIYALPMLTILTDKGTGIVTSVPSDAPDDYMALQDLKAKPAFRA 420
Query: 439 KYGVKDEWVLPYDIVPIIDIPEFGDRAAEKVCVDLKIKSQNEKDKLAEAKRLTYLKGFTD 498
K+GVKDEWVLP++IVPIIDIPE+GDRAAEKVC+DLKIKSQNEKDKLAEAKRL YL+GFT+
Sbjct: 421 KFGVKDEWVLPFEIVPIIDIPEYGDRAAEKVCLDLKIKSQNEKDKLAEAKRLVYLRGFTE 480
Query: 499 GTLIVGEFSGRKVQEAKPMIRSKLIETGQAIPYSEPEKRVTSRSGDECIVALTDQWYITY 558
GT+IVGE++GR+VQEAKP++R+KLIETGQAI YSEPEK+V SRSGDEC+VALTDQWYITY
Sbjct: 481 GTMIVGEYAGRRVQEAKPLLRTKLIETGQAIIYSEPEKKVMSRSGDECVVALTDQWYITY 540
Query: 559 GESEWKKLAEDCLANMNLFSDETRHGFQHTLSWLNQWACSRSFGLGTRIPWDEQFLVESL 618
GE EWKKL+E+CL+NMNL+SDETRHGF+HTL WLNQWACSRSFGLGTRIPWDE+FLVESL
Sbjct: 541 GEPEWKKLSEECLSNMNLYSDETRHGFEHTLGWLNQWACSRSFGLGTRIPWDEEFLVESL 600
Query: 619 SDSTIYMAYYTIAHLLHNGDLYGSGASAIKPEQMTDEVWDFVFCGAAEPKSTGISRSILN 678
SDSTIYMAYYT+AHLL NGD+YG + ++P QMTDEVW+F+FCG PKS+ I + LN
Sbjct: 601 SDSTIYMAYYTVAHLLQNGDMYGKSSDLVQPAQMTDEVWEFLFCGGPYPKSSNIPAATLN 660
Query: 679 KMKQEFEYWYPFDLRVSGKDLIQNHLTFCIYNHTAIMPEHHWPRGFRCNGHIMLNSEKMS 738
KMKQEFEYWYPFDLRVSGKDLIQNHLTFCIYNHTAIM + HWPRGFRCNGHIMLNSEKMS
Sbjct: 661 KMKQEFEYWYPFDLRVSGKDLIQNHLTFCIYNHTAIMSKDHWPRGFRCNGHIMLNSEKMS 720
Query: 739 KSTGNFRTLRQAIEEFSADATRFSLADAGDGVDDANFVFETANAAILRLTKEIAWMEDIL 798
KSTGNFRTLRQAIEEFSADATRFSLADAGDGVDDANFVFETAN AILRLTKEIAWME+IL
Sbjct: 721 KSTGNFRTLRQAIEEFSADATRFSLADAGDGVDDANFVFETANTAILRLTKEIAWMEEIL 780
Query: 799 QADSSSFLRTGPPSTYADRVFENEINIAVKTTEQNYKDYMFREALKSGFYDLQAARDEYR 858
A+SS LRTGPPSTYADRVFENEINIAVK TE+NY+DYMFREALK+GFYDLQ ARDEYR
Sbjct: 781 AAESS--LRTGPPSTYADRVFENEINIAVKMTEKNYRDYMFREALKTGFYDLQTARDEYR 840
Query: 859 FSCGAGGMNRDLVFRFMDVQTRLITPICPHYAEYVWREMLKKEGFVVNAGWPSAGSPDLT 918
FSCG+GGMNRDLV+RFMDVQTRLITP+CPHYAE+VWRE+LKK+GFV+ AGWPSAGSPDL
Sbjct: 841 FSCGSGGMNRDLVWRFMDVQTRLITPVCPHYAEFVWRELLKKDGFVIKAGWPSAGSPDLK 900
Query: 919 LKSANKYLQDSIVLMRKLLQKQLLGSKKGNKKGAPVTSVIEDKKLTGLIYVNEQFDGWKA 978
LKSANKYLQDSIVLMRKLL KQ+LGSKK NKKGAPVTS+ ED KL GLIYVNE+F+GW+A
Sbjct: 901 LKSANKYLQDSIVLMRKLLNKQILGSKKSNKKGAPVTSLSED-KLKGLIYVNEKFEGWQA 960
Query: 979 ECLRILRCKFDSTKRTFAPDSEIMEALQKSSVGQASDFRQTQKLCMPFLRFKKDEVAALG 1038
ECL+IL+ KFDS RTFA D EIM+ALQ+S+VGQA+DF++ QK CMPFLRFKKDE +G
Sbjct: 961 ECLQILQSKFDSNARTFASDGEIMKALQESTVGQAADFKKIQKQCMPFLRFKKDEAIKIG 1020
Query: 1039 VHALDLKLPFGEIEVLKENLELIKRQIGLEEVEVLCASDTDAIAKAGALASLLKQNPPSP 1098
V ALDLKLPFGEI+VLKENL+LIKRQ+GLEEVEVL +D+DA AKAG ASLL QNPPSP
Sbjct: 1021 VQALDLKLPFGEIDVLKENLDLIKRQVGLEEVEVLSFTDSDASAKAGTHASLLNQNPPSP 1080
Query: 1099 GNPTAIFL 1107
GNPTAIFL
Sbjct: 1081 GNPTAIFL 1085
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
XP_022948270.1 | 0.0e+00 | 100.00 | leucine--tRNA ligase, cytoplasmic-like [Cucurbita moschata] | [more] |
XP_023523654.1 | 0.0e+00 | 99.45 | leucine--tRNA ligase, cytoplasmic-like [Cucurbita pepo subsp. pepo] | [more] |
XP_022998939.1 | 0.0e+00 | 98.44 | leucine--tRNA ligase, cytoplasmic-like [Cucurbita maxima] | [more] |
XP_023521250.1 | 0.0e+00 | 96.06 | leucine--tRNA ligase, cytoplasmic-like [Cucurbita pepo subsp. pepo] >XP_02352125... | [more] |
XP_022970400.1 | 0.0e+00 | 94.13 | leucine--tRNA ligase, cytoplasmic-like [Cucurbita maxima] >XP_022970401.1 leucin... | [more] |
Match Name | E-value | Identity | Description | |
AT1G09620.1 | 0.0e+00 | 71.74 | ATP binding;leucine-tRNA ligases;aminoacyl-tRNA ligases;nucleotide binding;ATP b... | [more] |
AT2G31170.1 | 3.0e-05 | 29.92 | Cysteinyl-tRNA synthetase, class Ia family protein | [more] |
AT5G49030.1 | 1.5e-04 | 31.87 | tRNA synthetase class I (I, L, M and V) family protein | [more] |