CmoCh16G003190 (gene) Cucurbita moschata (Rifu)

NameCmoCh16G003190
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu))
DescriptionAspartate--tRNA(Asp/Asn) ligase
LocationCmo_Chr16 : 1458000 .. 1462345 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexonthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTCGTACAGCAGTATCCACAACTCTTTTACGCGCTCCGTATCAAGAAATTATATATTCCGTTAAGGACAACCCCTCGCGTAAATTGCTTTGAATGGGTAAATCAATCATTTGATCAGTCATCCTAAAATTAGGATTCATTTCTTGTCGCAAATATTCACTTGTACCATACCATATCAAAATGGACTGGCGCCAAGCCTCCTCAATTTCTCCCCATCTTCCACCACATTTCCCATATCCGCCCAAATCCGTTCGAATTCTCAGTTGCCTCTTAAAGACTCTCTTCTGTGGATCAATCGTACTGCGCTCTGCGGTGAATTGTCGGTTGACGATGTTGGGAAACGGGTTCGGCTGTGTGGTTGGGTCGCCCTGCATAGGGTTCACGGTGGTCTAACGTTTCTCAATCTTCGAGACCACAGTGGTATTGTTCAGGTGCTTATTTTTATGTGTTTTTGTTACTTCTGTTTGAATTCTTGGGACTGGCAAGTTCTATTGTTGTGAATTGGTTTTTACTGCTTGAATTAGGTGACTACGCTCCCCAACGAATTTCCAGATGCTCATTCGACTATTAATGACTTGCGGCTTGAGCATGTTGTTGCTGTAGAAGGCACTGTACGGTCTCGGCCTGGCGATTCAGTTAATGTAAAAATGAAAACAGGCTTGATAGAGGTATGTAAGAACTTTGATTTTATTTTACTGTTCATGTAGCCTGGCATTGGATTGGAAATGTTTTGACGAACACGACTCTCCACAATAGTATGATATTGTCCACTTTGAGCATAAGCTCTCGTGGCTTTGCTTTGGACTTCCCCAAAAGGCCTCGTACCAATGAAGAGAGTATTCTTTGATTATAAACCCATGATCATTCCCTAAATTAGTCGACGTGGGACTTTCATCATCCAACATTCAGTTTTCTCGCTCATTAAATTATGCTCATGCCTGCCCTCCACGGTCCCCATGGCCATCTCCGAATGAACAAGAATTTTAAGCTAGATTTTGAATTTTCAAGTATTTAATCGCAGGCTTGAACCTAGTATGTTGGAGGAATTTCCGAACATCACAAGCTTTTGCAAAATAAATTCATTCATTCTTGCTAAGAAACAATTCTGGGAGTTACTGGTCACGGTTGTTAAAGATAGATCAGTCGAAGTGATTAGTTCTTTTGGGTGTCCTGGAGTCGCCACATAAATGCTGCTTCTAGCATGGAACACTTGTTTTGGTTGCATGGTATATTTATCTCAACTTGAAGGCAAGAATTCTAAGGTTGTAGCCATAAAAGCACCCTTTTTAAAGGGGAAACTTCAGAATTTTTGAAGGAGGGGATAGAGATGGGGTTTGTGGGATTTAATTTTATAGTTTTATTTTGATAGTTTTATGGAAATCGTTCTCTGTCACGCTCTTTATTGGTGTAAATCCTCACATCTCTTTAAGGATTAAAGTCTTTCTACTTCACTCACCTGTTGGAAATCCTTTTTGTATCCACCTACGGTGGAGTGAGCCCTTTTGTACATTTCATTCATCAATGAAATCAACGTCAGATTAAAACGAGCTGAGCATTTATAGGTACCGGTACCTTGATCTACGAAGGAAGCAAATGAATTTTAACATAATGCTCCGGCATAAAGTGGTGAAGCTCATGCGTAGATATTTAGAGGATGTACAGCTCTGTTTATAATTTTAACATTTTTGGCCTTTTTTGATGTTTTCTTGAATAATTCTCTGCATTAGAATTTTTACATTATACTGTATGGATGGTTAGAAATTGAATTTTTATAGCCATACTTCGATTCTGAACTTTTGATGGTTATAAGGTAGATTGAGACGCCAATCCTTTCTAGATCTACTCCAGAAGGTGCTCGGGATTATCTAGTGCCGTCTAGAGTTCAGGTAGCCTATATTGACTCAACTTCTTATTGATTCTGATTGCTCTCGAGGCGGGTTGTTTTGGTAACTAGTATTTTTCAATCCAAAAACTTAAAATAATTTTCTTGAGAAATCTGAATGTGATGCCATTTTCAGTTTCTAGCTATGTTCTTTATTGCTTTTTTATTTGGGTAGTTGGTATACCTTGTTAATTCATATCACAGTAAGTGGTAAGTAACTTGTAGAGCATAATATTCATTCCCTTTTAAGACATTTCGTGCCAAAAGTTTATAAACGGAAATGGAGGTGTTAAGAAAGTGCAGTTAGGTCCACATTTTTTATGTTTTTCTGTATTCGCAGAATGCCTATTCTTTGAGGTGGTGAAGTTAATTACCTTTTCCTCAAAAAAAAAAAACATAATTGTTGTTTCCCGACAAAGAAGATAACAATATAACATAATTGTGTTCATTGCTTTTGTTGCTGTACATTGGTGGAGGGAGTTTGAGAATTCACAAACGTGAAGTTCAAGAGAAGGTTCTGGAGATTGTGGGAATCTCTCATGAACAGGTAAAAGCTAAAAGGGTTTCATGGTTCATTAATTTGGTTTGGTCCCATTAATTTATGAGGCCGAACTGATTGTTTGGTTCAGCTGGTCCAGGGAAGATATTATCATCTCCTTCAATATTATTAGTTTTTCTTGTTCTTTTCTGGCTTGGCAGGCAGAAGCGAAGTTCGGTTATTTATTGGAGGCACTAGACATGGGTGCACCCCCTCATGGTATTAACCATTAAAGCTTTCTTCCAGTGCCTAAAACTTTAAGTCATCCTTATTGTTTAAATGCATTTCAATCATGCCAGTACTGATAATATATTGAAATTTTTAATATTGGGTTGCCTATTTTGATACTAAATCTGGTTCTTTATAAGTTCTTTTAGGGGAGACTTGGAGAACTTTCTTAGGTTTTTTTTTTATTTTTGCATCTGATCTGACTTATGAGAGAATAAGATGAGGAAAGAGCTTTCTATTCAAGGGATTATGGAGAGTTTCTACTTGGTGTTTATGTTAGATTTATAGATTATTTGTCCAATTACGAAGTAGTTTACAATATTTATTCAACCTCTAGGAGACTAGGATTGGAAAATCAGCCAGGGGTGGGCCTTTCAGTAGGGCTTGAAATGACCATAGATATCTTTGAAGACCCCATGTGGAAGATTTAATGATCTAAATTGTTTGTTTGTCTTCCAAATTTGGTTTCGGGTGAAGCTTCCTCCAACATTAGTGTAACAGATATTGTCCGCTTTAGTCCGTTATGCGTTTTCTATGGAAAGGGTCTAGCCCTACTCCGACTGGTGCCTCGCACCGTCCAGTGACGGGCTCTAATATCATTTGTAATTGTCCAAACCCACTACTAGCAGATATTGTCCTCTTTGAGCTTTCCCTTTCAGCCTTCCCCTCAAGGTTTTTTTAAAACGCGTCTACTAGGAAGAGGTTTTCAAATCTTTATAAAAAATGTTTCGTTTCCCTCTCCAACCGATGTGGGATCTCTCAATTAGTGGGTTGTGAGCATAATACAAAGCGGGTCCTCAAATACTTGGTTACCAATAGAAAGAACGAAACAGAAAAAAGAAGTGCAATATGAATATCGGTGTGGTAGTGGACCGACTAAATAGATGAATGGTGTATTCTATTCGGGATAATAATCGGTGCGTTTATCTCTACTAAGCCAGGCTCATATTGACTTCTATTTCATATACATGACTAGTGGTCCTCATAGCCATTAATAATATGTCTATTTCTTTTGTTTTAACCTTTTTTCCCCTTGGTGCAGGGGGCATTGCTTATGGGTTGGACAGATTAGTCATGTTATTGGCAGAGGCAAATTCCATTAGGGATGTCATAGCTTTCCCAAAGACAACAACAGCACAATGTGCCCTCACAAAAGCTCCTTCAGAAGTAGATCCCCAACAACTCAAGGATTTATCATATCAGAACCAATAAGTTTACGTTCAGGTGCTCGTCCAGTCTGGTTCGGTTCAACTTTTAATTCTATATTCACCTCACTCGGCCCGTAATTTTTCGACTCAATGGCCTTGCATGTTTTGAATCCGTGTTAGGATGTGATGAGCAATAAACACAAATTTTTGTAAGTTAACGAAGTTCATCAAATCCCGTTAACAGGATCGGAGTTCTTCTAGTTACAACGTCGAACATGTTGACCTAATTTAGTGATGGGCGTTTTAGTTTGATGTCATACACAGTATAGGTATAGTGTGCTGTGTTTGGCTGTTACTTAATGTCTTTTAATTTGGTTGGGCCGTTTTTGGTGCTTGTGGTTTTGGTGCAGACAGGCGATTATCGGTTGTCAAGCAAAAGGTAGAAGCATGTGGATAAGAAGATGGGTGTGCAGATTGCTTCGATTCTCTTACCATAAAAAGGTAAACAATAAACAGAATTATTGAGGAATTAATTGTCTTGTCAGGGT

mRNA sequence

ATGTTCGTACAGCAGATTCATTTCTTGTCGCAAATATTCACTTGTACCATACCATATCAAAATGGACTGGCGCCAAGCCTCCTCAATTTCTCCCCATCTTCCACCACATTTCCCATATCCGCCCAAATCCGTTCGAATTCTCAGTTGCCTCTTAAAGACTCTCTTCTGTGGATCAATCGTACTGCGCTCTGCGGTGAATTGTCGGTTGACGATGTTGGGAAACGGGTTCGGCTGTGTGGTTGGGTCGCCCTGCATAGGGTTCACGGTGGTCTAACGTTTCTCAATCTTCGAGACCACAGTGGTATTGTTCAGGTGACTACGCTCCCCAACGAATTTCCAGATGCTCATTCGACTATTAATGACTTGCGGCTTGAGCATGTTGTTGCTGTAGAAGGCACTGTACGGTCTCGGCCTGGCGATTCAGTTAATGTAAAAATGAAAACAGGCTTGATAGAGATTGAGACGCCAATCCTTTCTAGATCTACTCCAGAAGGTGCTCGGGATTATCTAGTGCCGTCTAGAGTTCAGGCAGAAGCGAAGTTCGGTTATTTATTGGAGGCACTAGACATGGGTGCACCCCCTCATGGGGGCATTGCTTATGGGTTGGACAGATTAGTCATGTTATTGGCAGAGGCAAATTCCATTAGGGATGTCATAGCTTTCCCAAAGACAACAACAGCACAATGTGCCCTCACAAAAGCTCCTTCAGAAGTAGATCCCCAACAACTCAAGGATTTATCATATCAGAACCAATAAGTTTACGTTCAGACAGGCGATTATCGGTTGTCAAGCAAAAGGTAGAAGCATGTGGATAAGAAGATGGGTGTGCAGATTGCTTCGATTCTCTTACCATAAAAAGGTAAACAATAAACAGAATTATTGAGGAATTAATTGTCTTGTCAGGGT

Coding sequence (CDS)

ATGTTCGTACAGCAGATTCATTTCTTGTCGCAAATATTCACTTGTACCATACCATATCAAAATGGACTGGCGCCAAGCCTCCTCAATTTCTCCCCATCTTCCACCACATTTCCCATATCCGCCCAAATCCGTTCGAATTCTCAGTTGCCTCTTAAAGACTCTCTTCTGTGGATCAATCGTACTGCGCTCTGCGGTGAATTGTCGGTTGACGATGTTGGGAAACGGGTTCGGCTGTGTGGTTGGGTCGCCCTGCATAGGGTTCACGGTGGTCTAACGTTTCTCAATCTTCGAGACCACAGTGGTATTGTTCAGGTGACTACGCTCCCCAACGAATTTCCAGATGCTCATTCGACTATTAATGACTTGCGGCTTGAGCATGTTGTTGCTGTAGAAGGCACTGTACGGTCTCGGCCTGGCGATTCAGTTAATGTAAAAATGAAAACAGGCTTGATAGAGATTGAGACGCCAATCCTTTCTAGATCTACTCCAGAAGGTGCTCGGGATTATCTAGTGCCGTCTAGAGTTCAGGCAGAAGCGAAGTTCGGTTATTTATTGGAGGCACTAGACATGGGTGCACCCCCTCATGGGGGCATTGCTTATGGGTTGGACAGATTAGTCATGTTATTGGCAGAGGCAAATTCCATTAGGGATGTCATAGCTTTCCCAAAGACAACAACAGCACAATGTGCCCTCACAAAAGCTCCTTCAGAAGTAGATCCCCAACAACTCAAGGATTTATCATATCAGAACCAATAA
BLAST of CmoCh16G003190 vs. Swiss-Prot
Match: SYDM_ARATH (Aspartate--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana GN=At4g33760 PE=2 SV=1)

HSP 1 Score: 172.9 bits (437), Expect = 4.3e-42
Identity = 78/99 (78.79%), Postives = 89/99 (89.90%), Query Frame = 1

Query: 55  LLWINRTALCGELSVDDVGKRVRLCGWVALHRVHGGLTFLNLRDHSGIVQVTTLPNEFPD 114
           L W++RT LCGELSV+DVGKRV LCGWVALHRVHGGLTFLNLRDH+GIVQV TLP+EFP+
Sbjct: 70  LRWVSRTELCGELSVNDVGKRVHLCGWVALHRVHGGLTFLNLRDHTGIVQVRTLPDEFPE 129

Query: 115 AHSTINDLRLEHVVAVEGTVRSRPGDSVNVKMKTGLIEI 154
           AH  IND+RLE+VV VEGTVRSRP +SVN KMKTG +E+
Sbjct: 130 AHGLINDMRLEYVVLVEGTVRSRPNESVNKKMKTGFVEV 168

BLAST of CmoCh16G003190 vs. Swiss-Prot
Match: SYDND_THEEB (Aspartate--tRNA(Asp/Asn) ligase OS=Thermosynechococcus elongatus (strain BP-1) GN=aspS PE=3 SV=1)

HSP 1 Score: 109.0 bits (271), Expect = 7.6e-23
Identity = 58/98 (59.18%), Postives = 67/98 (68.37%), Query Frame = 1

Query: 149 GLIEIETPILSRSTPEGARDYLVPSRVQAEAKFGYLLEALDMGAPPHGGIAYGLDRLVML 208
           G + I  P L R   E     +      A+ KFG+LLEA + G PPHGGIAYGLDRLVML
Sbjct: 492 GSLRIHQPELQRQVFE----IIGIDEATAQEKFGFLLEAFEFGTPPHGGIAYGLDRLVML 551

Query: 209 LAEANSIRDVIAFPKTTTAQCALTKAPSEVDPQQLKDL 247
           LA A+SIRD IAFPKT  A+C LT APS V+PQQLK+L
Sbjct: 552 LAGADSIRDTIAFPKTQQARCLLTGAPSSVEPQQLKEL 585

BLAST of CmoCh16G003190 vs. Swiss-Prot
Match: SYDND_METFK (Aspartate--tRNA(Asp/Asn) ligase OS=Methylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875) GN=aspS PE=3 SV=1)

HSP 1 Score: 108.2 bits (269), Expect = 1.3e-22
Identity = 52/79 (65.82%), Postives = 65/79 (82.28%), Query Frame = 1

Query: 168 DYLVPSRVQAEAKFGYLLEALDMGAPPHGGIAYGLDRLVMLLAEANSIRDVIAFPKTTTA 227
           D L  S+ +A+ KFG+LL+AL  GAPPHGG+A+GLDRLV L+A A SIRDVIAFPKT  A
Sbjct: 505 DALKISKEEAQEKFGFLLDALQYGAPPHGGLAFGLDRLVTLMAGAESIRDVIAFPKTQRA 564

Query: 228 QCALTKAPSEVDPQQLKDL 247
           QC +TKAP+EVD +QL++L
Sbjct: 565 QCLMTKAPNEVDEKQLREL 583

BLAST of CmoCh16G003190 vs. Swiss-Prot
Match: SYDND_BURXL (Aspartate--tRNA(Asp/Asn) ligase OS=Burkholderia xenovorans (strain LB400) GN=aspS PE=3 SV=1)

HSP 1 Score: 107.5 bits (267), Expect = 2.2e-22
Identity = 50/71 (70.42%), Postives = 63/71 (88.73%), Query Frame = 1

Query: 176 QAEAKFGYLLEALDMGAPPHGGIAYGLDRLVMLLAEANSIRDVIAFPKTTTAQCALTKAP 235
           +A+AKFG+LL+AL  GAPPHGGIA+GLDR+V ++A A+SIRDVIAFPKT  AQC LT+AP
Sbjct: 518 EAQAKFGFLLDALQYGAPPHGGIAFGLDRIVTMMAGADSIRDVIAFPKTQRAQCLLTQAP 577

Query: 236 SEVDPQQLKDL 247
           SEVD +QL++L
Sbjct: 578 SEVDERQLREL 588

BLAST of CmoCh16G003190 vs. Swiss-Prot
Match: SYDND_PARPJ (Aspartate--tRNA(Asp/Asn) ligase OS=Paraburkholderia phytofirmans (strain DSM 17436 / LMG 22146 / PsJN) GN=aspS PE=3 SV=1)

HSP 1 Score: 107.5 bits (267), Expect = 2.2e-22
Identity = 50/71 (70.42%), Postives = 63/71 (88.73%), Query Frame = 1

Query: 176 QAEAKFGYLLEALDMGAPPHGGIAYGLDRLVMLLAEANSIRDVIAFPKTTTAQCALTKAP 235
           +A+AKFG+LL+AL  GAPPHGGIA+GLDR+V ++A A+SIRDVIAFPKT  AQC LT+AP
Sbjct: 518 EAQAKFGFLLDALQYGAPPHGGIAFGLDRIVTMMAGADSIRDVIAFPKTQRAQCLLTQAP 577

Query: 236 SEVDPQQLKDL 247
           SEVD +QL++L
Sbjct: 578 SEVDERQLREL 588

BLAST of CmoCh16G003190 vs. TrEMBL
Match: A0A0A0KT15_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G000540 PE=4 SV=1)

HSP 1 Score: 216.9 bits (551), Expect = 2.9e-53
Identity = 109/129 (84.50%), Postives = 113/129 (87.60%), Query Frame = 1

Query: 29  NFSPSSTTFPISAQI----RSNSQLPLKDSLLWINRTALCGELSVDDVGKRVRLCGWVAL 88
           +FS S+T  P SA       SN   P KDSL WINRTALCGELSVDDVGKRVRLCGWVAL
Sbjct: 46  SFSTSATPSPSSATEILVPTSNPHSPFKDSLQWINRTALCGELSVDDVGKRVRLCGWVAL 105

Query: 89  HRVHGGLTFLNLRDHSGIVQVTTLPNEFPDAHSTINDLRLEHVVAVEGTVRSRPGDSVNV 148
           HRVHGGLTFLNLRDHSGIVQVTTLPNEFPDAHSTINDLRLE+VVAVEGTVRSRPGDSVNV
Sbjct: 106 HRVHGGLTFLNLRDHSGIVQVTTLPNEFPDAHSTINDLRLEYVVAVEGTVRSRPGDSVNV 165

Query: 149 KMKTGLIEI 154
           KMKTGLIE+
Sbjct: 166 KMKTGLIEV 174

BLAST of CmoCh16G003190 vs. TrEMBL
Match: E5GBH9_CUCME (Aspartyl-tRNA synthetase OS=Cucumis melo subsp. melo PE=4 SV=1)

HSP 1 Score: 214.5 bits (545), Expect = 1.4e-52
Identity = 108/129 (83.72%), Postives = 112/129 (86.82%), Query Frame = 1

Query: 29  NFSPSSTTFPISAQIR----SNSQLPLKDSLLWINRTALCGELSVDDVGKRVRLCGWVAL 88
           +FS S+T  P SA       SNS  P KDSL WINRTALCGELSVDDVGKRVRLCGWVAL
Sbjct: 46  SFSTSATPPPSSATETLVPTSNSHSPFKDSLQWINRTALCGELSVDDVGKRVRLCGWVAL 105

Query: 89  HRVHGGLTFLNLRDHSGIVQVTTLPNEFPDAHSTINDLRLEHVVAVEGTVRSRPGDSVNV 148
           HRVHGGLTFLNLRDHSGIVQVTTLPNEFPDAHS INDLRLE+VVAVEGTVRSRPGDSVNV
Sbjct: 106 HRVHGGLTFLNLRDHSGIVQVTTLPNEFPDAHSAINDLRLEYVVAVEGTVRSRPGDSVNV 165

Query: 149 KMKTGLIEI 154
           KMKTG IE+
Sbjct: 166 KMKTGFIEV 174

BLAST of CmoCh16G003190 vs. TrEMBL
Match: A0A151T3C0_CAJCA (Aspartyl-tRNA synthetase OS=Cajanus cajan GN=KK1_016047 PE=4 SV=1)

HSP 1 Score: 191.0 bits (484), Expect = 1.7e-45
Identity = 96/151 (63.58%), Postives = 120/151 (79.47%), Query Frame = 1

Query: 10  SQIFTCTIPYQNGLAPS----LLNFSPSSTTFPISAQ-IRSNSQLP--LKDSLLWINRTA 69
           S  F C +P    L  S    + + S S+ T P +++ + SN++ P  L +SL W+NRTA
Sbjct: 18  SLAFLCRVPSAIPLTLSRTRNVFSVSASTQTSPSTSETLTSNAKPPFALSESLEWVNRTA 77

Query: 70  LCGELSVDDVGKRVRLCGWVALHRVHGGLTFLNLRDHSGIVQVTTLPNEFPDAHSTINDL 129
           LCGEL+++DVGK+V+LCGWVALHRVHGGLTFLNLRDH+GIVQVTTLP+EFPDAHS INDL
Sbjct: 78  LCGELTLNDVGKKVQLCGWVALHRVHGGLTFLNLRDHTGIVQVTTLPDEFPDAHSAINDL 137

Query: 130 RLEHVVAVEGTVRSRPGDSVNVKMKTGLIEI 154
           RLE+VVA+EG VRSRP +S+N KMKTGLIE+
Sbjct: 138 RLEYVVAIEGVVRSRPNESINKKMKTGLIEV 168

BLAST of CmoCh16G003190 vs. TrEMBL
Match: B9S7X4_RICCO (Aspartyl-tRNA synthetase, putative OS=Ricinus communis GN=RCOM_1382380 PE=4 SV=1)

HSP 1 Score: 190.3 bits (482), Expect = 2.9e-45
Identity = 91/124 (73.39%), Postives = 106/124 (85.48%), Query Frame = 1

Query: 31  SPSSTTFPISAQIRSNS-QLPLKDSLLWINRTALCGELSVDDVGKRVRLCGWVALHRVHG 90
           S S+ T PIS+Q +     +P K++L W++RT LCGELSV+DVGKRV LCGWVALHRVHG
Sbjct: 50  SSSTDTIPISSQQKQQQPSVPFKETLNWVSRTGLCGELSVNDVGKRVLLCGWVALHRVHG 109

Query: 91  GLTFLNLRDHSGIVQVTTLPNEFPDAHSTINDLRLEHVVAVEGTVRSRPGDSVNVKMKTG 150
           GLTFLNLRDH+G VQVTTLP+EFPDAHS INDLRLE+VVAVEG VRSRP +SVN KMKTG
Sbjct: 110 GLTFLNLRDHTGTVQVTTLPDEFPDAHSIINDLRLEYVVAVEGVVRSRPTESVNKKMKTG 169

Query: 151 LIEI 154
           L+E+
Sbjct: 170 LVEV 173

BLAST of CmoCh16G003190 vs. TrEMBL
Match: A0A0S3QWN3_PHAAN (Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.01G019300 PE=4 SV=1)

HSP 1 Score: 188.7 bits (478), Expect = 8.4e-45
Identity = 90/126 (71.43%), Postives = 108/126 (85.71%), Query Frame = 1

Query: 31  SPSSTTFPISAQIRSNSQLP---LKDSLLWINRTALCGELSVDDVGKRVRLCGWVALHRV 90
           +PS+   P +++I +++  P   L +SL WINRTALCGELS +DVGKRV+LCGWVALHRV
Sbjct: 47  TPSTQPLPSASEILTSNAKPPLALTESLEWINRTALCGELSSNDVGKRVQLCGWVALHRV 106

Query: 91  HGGLTFLNLRDHSGIVQVTTLPNEFPDAHSTINDLRLEHVVAVEGTVRSRPGDSVNVKMK 150
           HGGLTFLNLRDHSGIVQVTTLP+EFPDAHS INDLRLE+VVA++G VRSRP +S+N KMK
Sbjct: 107 HGGLTFLNLRDHSGIVQVTTLPDEFPDAHSAINDLRLEYVVAIDGVVRSRPSESINKKMK 166

Query: 151 TGLIEI 154
           TG IE+
Sbjct: 167 TGSIEV 172

BLAST of CmoCh16G003190 vs. TAIR10
Match: AT4G33760.1 (AT4G33760.1 tRNA synthetase class II (D, K and N) family protein)

HSP 1 Score: 172.9 bits (437), Expect = 2.4e-43
Identity = 78/99 (78.79%), Postives = 89/99 (89.90%), Query Frame = 1

Query: 55  LLWINRTALCGELSVDDVGKRVRLCGWVALHRVHGGLTFLNLRDHSGIVQVTTLPNEFPD 114
           L W++RT LCGELSV+DVGKRV LCGWVALHRVHGGLTFLNLRDH+GIVQV TLP+EFP+
Sbjct: 70  LRWVSRTELCGELSVNDVGKRVHLCGWVALHRVHGGLTFLNLRDHTGIVQVRTLPDEFPE 129

Query: 115 AHSTINDLRLEHVVAVEGTVRSRPGDSVNVKMKTGLIEI 154
           AH  IND+RLE+VV VEGTVRSRP +SVN KMKTG +E+
Sbjct: 130 AHGLINDMRLEYVVLVEGTVRSRPNESVNKKMKTGFVEV 168

BLAST of CmoCh16G003190 vs. TAIR10
Match: AT3G13490.1 (AT3G13490.1 Lysyl-tRNA synthetase, class II)

HSP 1 Score: 50.4 bits (119), Expect = 1.8e-06
Identity = 24/38 (63.16%), Postives = 28/38 (73.68%), Query Frame = 1

Query: 185 LEALDMGAPPHGGIAYGLDRLVMLLAEANSIRDVIAFP 223
           L AL+ G PP  G+  G+DRLVMLL  + SIRDVIAFP
Sbjct: 559 LTALEYGMPPASGMGLGIDRLVMLLTNSASIRDVIAFP 596

BLAST of CmoCh16G003190 vs. NCBI nr
Match: gi|778689110|ref|XP_004150278.2| (PREDICTED: aspartate--tRNA ligase, mitochondrial [Cucumis sativus])

HSP 1 Score: 216.9 bits (551), Expect = 4.2e-53
Identity = 109/129 (84.50%), Postives = 113/129 (87.60%), Query Frame = 1

Query: 29  NFSPSSTTFPISAQI----RSNSQLPLKDSLLWINRTALCGELSVDDVGKRVRLCGWVAL 88
           +FS S+T  P SA       SN   P KDSL WINRTALCGELSVDDVGKRVRLCGWVAL
Sbjct: 46  SFSTSATPSPSSATEILVPTSNPHSPFKDSLQWINRTALCGELSVDDVGKRVRLCGWVAL 105

Query: 89  HRVHGGLTFLNLRDHSGIVQVTTLPNEFPDAHSTINDLRLEHVVAVEGTVRSRPGDSVNV 148
           HRVHGGLTFLNLRDHSGIVQVTTLPNEFPDAHSTINDLRLE+VVAVEGTVRSRPGDSVNV
Sbjct: 106 HRVHGGLTFLNLRDHSGIVQVTTLPNEFPDAHSTINDLRLEYVVAVEGTVRSRPGDSVNV 165

Query: 149 KMKTGLIEI 154
           KMKTGLIE+
Sbjct: 166 KMKTGLIEV 174

BLAST of CmoCh16G003190 vs. NCBI nr
Match: gi|307135961|gb|ADN33821.1| (aspartyl-tRNA synthetase [Cucumis melo subsp. melo])

HSP 1 Score: 214.5 bits (545), Expect = 2.1e-52
Identity = 108/129 (83.72%), Postives = 112/129 (86.82%), Query Frame = 1

Query: 29  NFSPSSTTFPISAQIR----SNSQLPLKDSLLWINRTALCGELSVDDVGKRVRLCGWVAL 88
           +FS S+T  P SA       SNS  P KDSL WINRTALCGELSVDDVGKRVRLCGWVAL
Sbjct: 46  SFSTSATPPPSSATETLVPTSNSHSPFKDSLQWINRTALCGELSVDDVGKRVRLCGWVAL 105

Query: 89  HRVHGGLTFLNLRDHSGIVQVTTLPNEFPDAHSTINDLRLEHVVAVEGTVRSRPGDSVNV 148
           HRVHGGLTFLNLRDHSGIVQVTTLPNEFPDAHS INDLRLE+VVAVEGTVRSRPGDSVNV
Sbjct: 106 HRVHGGLTFLNLRDHSGIVQVTTLPNEFPDAHSAINDLRLEYVVAVEGTVRSRPGDSVNV 165

Query: 149 KMKTGLIEI 154
           KMKTG IE+
Sbjct: 166 KMKTGFIEV 174

BLAST of CmoCh16G003190 vs. NCBI nr
Match: gi|659108863|ref|XP_008454426.1| (PREDICTED: aspartate--tRNA ligase, mitochondrial isoform X1 [Cucumis melo])

HSP 1 Score: 214.5 bits (545), Expect = 2.1e-52
Identity = 108/129 (83.72%), Postives = 112/129 (86.82%), Query Frame = 1

Query: 29  NFSPSSTTFPISAQIR----SNSQLPLKDSLLWINRTALCGELSVDDVGKRVRLCGWVAL 88
           +FS S+T  P SA       SNS  P KDSL WINRTALCGELSVDDVGKRVRLCGWVAL
Sbjct: 46  SFSTSATPPPSSATETLVPTSNSHSPFKDSLQWINRTALCGELSVDDVGKRVRLCGWVAL 105

Query: 89  HRVHGGLTFLNLRDHSGIVQVTTLPNEFPDAHSTINDLRLEHVVAVEGTVRSRPGDSVNV 148
           HRVHGGLTFLNLRDHSGIVQVTTLPNEFPDAHS INDLRLE+VVAVEGTVRSRPGDSVNV
Sbjct: 106 HRVHGGLTFLNLRDHSGIVQVTTLPNEFPDAHSAINDLRLEYVVAVEGTVRSRPGDSVNV 165

Query: 149 KMKTGLIEI 154
           KMKTG IE+
Sbjct: 166 KMKTGFIEV 174

BLAST of CmoCh16G003190 vs. NCBI nr
Match: gi|1012350364|gb|KYP61553.1| (Aspartyl-tRNA synthetase [Cajanus cajan])

HSP 1 Score: 191.0 bits (484), Expect = 2.4e-45
Identity = 96/151 (63.58%), Postives = 120/151 (79.47%), Query Frame = 1

Query: 10  SQIFTCTIPYQNGLAPS----LLNFSPSSTTFPISAQ-IRSNSQLP--LKDSLLWINRTA 69
           S  F C +P    L  S    + + S S+ T P +++ + SN++ P  L +SL W+NRTA
Sbjct: 18  SLAFLCRVPSAIPLTLSRTRNVFSVSASTQTSPSTSETLTSNAKPPFALSESLEWVNRTA 77

Query: 70  LCGELSVDDVGKRVRLCGWVALHRVHGGLTFLNLRDHSGIVQVTTLPNEFPDAHSTINDL 129
           LCGEL+++DVGK+V+LCGWVALHRVHGGLTFLNLRDH+GIVQVTTLP+EFPDAHS INDL
Sbjct: 78  LCGELTLNDVGKKVQLCGWVALHRVHGGLTFLNLRDHTGIVQVTTLPDEFPDAHSAINDL 137

Query: 130 RLEHVVAVEGTVRSRPGDSVNVKMKTGLIEI 154
           RLE+VVA+EG VRSRP +S+N KMKTGLIE+
Sbjct: 138 RLEYVVAIEGVVRSRPNESINKKMKTGLIEV 168

BLAST of CmoCh16G003190 vs. NCBI nr
Match: gi|255562164|ref|XP_002522090.1| (PREDICTED: aspartate--tRNA ligase, chloroplastic/mitochondrial [Ricinus communis])

HSP 1 Score: 190.3 bits (482), Expect = 4.2e-45
Identity = 91/124 (73.39%), Postives = 106/124 (85.48%), Query Frame = 1

Query: 31  SPSSTTFPISAQIRSNS-QLPLKDSLLWINRTALCGELSVDDVGKRVRLCGWVALHRVHG 90
           S S+ T PIS+Q +     +P K++L W++RT LCGELSV+DVGKRV LCGWVALHRVHG
Sbjct: 50  SSSTDTIPISSQQKQQQPSVPFKETLNWVSRTGLCGELSVNDVGKRVLLCGWVALHRVHG 109

Query: 91  GLTFLNLRDHSGIVQVTTLPNEFPDAHSTINDLRLEHVVAVEGTVRSRPGDSVNVKMKTG 150
           GLTFLNLRDH+G VQVTTLP+EFPDAHS INDLRLE+VVAVEG VRSRP +SVN KMKTG
Sbjct: 110 GLTFLNLRDHTGTVQVTTLPDEFPDAHSIINDLRLEYVVAVEGVVRSRPTESVNKKMKTG 169

Query: 151 LIEI 154
           L+E+
Sbjct: 170 LVEV 173

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
SYDM_ARATH4.3e-4278.79Aspartate--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana GN=A... [more]
SYDND_THEEB7.6e-2359.18Aspartate--tRNA(Asp/Asn) ligase OS=Thermosynechococcus elongatus (strain BP-1) G... [more]
SYDND_METFK1.3e-2265.82Aspartate--tRNA(Asp/Asn) ligase OS=Methylobacillus flagellatus (strain KT / ATCC... [more]
SYDND_BURXL2.2e-2270.42Aspartate--tRNA(Asp/Asn) ligase OS=Burkholderia xenovorans (strain LB400) GN=asp... [more]
SYDND_PARPJ2.2e-2270.42Aspartate--tRNA(Asp/Asn) ligase OS=Paraburkholderia phytofirmans (strain DSM 174... [more]
Match NameE-valueIdentityDescription
A0A0A0KT15_CUCSA2.9e-5384.50Uncharacterized protein OS=Cucumis sativus GN=Csa_4G000540 PE=4 SV=1[more]
E5GBH9_CUCME1.4e-5283.72Aspartyl-tRNA synthetase OS=Cucumis melo subsp. melo PE=4 SV=1[more]
A0A151T3C0_CAJCA1.7e-4563.58Aspartyl-tRNA synthetase OS=Cajanus cajan GN=KK1_016047 PE=4 SV=1[more]
B9S7X4_RICCO2.9e-4573.39Aspartyl-tRNA synthetase, putative OS=Ricinus communis GN=RCOM_1382380 PE=4 SV=1[more]
A0A0S3QWN3_PHAAN8.4e-4571.43Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.01G019300 PE=... [more]
Match NameE-valueIdentityDescription
AT4G33760.12.4e-4378.79 tRNA synthetase class II (D, K and N) family protein[more]
AT3G13490.11.8e-0663.16 Lysyl-tRNA synthetase, class II[more]
Match NameE-valueIdentityDescription
gi|778689110|ref|XP_004150278.2|4.2e-5384.50PREDICTED: aspartate--tRNA ligase, mitochondrial [Cucumis sativus][more]
gi|307135961|gb|ADN33821.1|2.1e-5283.72aspartyl-tRNA synthetase [Cucumis melo subsp. melo][more]
gi|659108863|ref|XP_008454426.1|2.1e-5283.72PREDICTED: aspartate--tRNA ligase, mitochondrial isoform X1 [Cucumis melo][more]
gi|1012350364|gb|KYP61553.1|2.4e-4563.58Aspartyl-tRNA synthetase [Cajanus cajan][more]
gi|255562164|ref|XP_002522090.1|4.2e-4573.39PREDICTED: aspartate--tRNA ligase, chloroplastic/mitochondrial [Ricinus communis... [more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR004364aa-tRNA-synt_II
IPR004365NA-bd_OB_tRNA
IPR004524Asp-tRNA-ligase_1
IPR012340NA-bd_OB-fold
IPR018150Aminoacyl-tRNA synthetase, class II (D/K/N)-like
Vocabulary: Molecular Function
TermDefinition
GO:0000166nucleotide binding
GO:0004812aminoacyl-tRNA ligase activity
GO:0005524ATP binding
GO:0003676nucleic acid binding
GO:0016874ligase activity
Vocabulary: Biological Process
TermDefinition
GO:0006418tRNA aminoacylation for protein translation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006522 alanine metabolic process
biological_process GO:0006531 aspartate metabolic process
biological_process GO:0006422 aspartyl-tRNA aminoacylation
biological_process GO:0009658 chloroplast organization
biological_process GO:0048481 plant ovule development
biological_process GO:0006364 rRNA processing
biological_process GO:0006418 tRNA aminoacylation for protein translation
cellular_component GO:0005739 mitochondrion
cellular_component GO:0016020 membrane
cellular_component GO:0009507 chloroplast
cellular_component GO:0005737 cytoplasm
molecular_function GO:0004815 aspartate-tRNA ligase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004812 aminoacyl-tRNA ligase activity
molecular_function GO:0016874 ligase activity
molecular_function GO:0000166 nucleotide binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh16G003190.1CmoCh16G003190.1mRNA


Analysis Name: InterPro Annotations of Cucurbita moschata
Date Performed: 2017-05-19
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004364Aminoacyl-tRNA synthetase, class II (D/K/N)PFAMPF00152tRNA-synt_2coord: 176..225
score: 9.2
IPR004365OB-fold nucleic acid binding domain, AA-tRNA synthetase-typePFAMPF01336tRNA_anti-codoncoord: 76..145
score: 2.5
IPR004524Aspartate-tRNA ligase, bacterial/mitochondrial-typePANTHERPTHR22594:SF5ASPARTATE--TRNA LIGASE, MITOCHONDRIALcoord: 176..238
score: 5.3
IPR012340Nucleic acid-binding, OB-foldGENE3DG3DSA:2.40.50.140coord: 60..150
score: 6.4
IPR012340Nucleic acid-binding, OB-foldunknownSSF50249Nucleic acid-binding proteinscoord: 60..154
score: 6.64
IPR018150Aminoacyl-tRNA synthetase, class II (D/K/N)-likePANTHERPTHR22594ASPARTYL/LYSYL-TRNA SYNTHETASEcoord: 176..238
score: 5.3
NoneNo IPR availableGENE3DG3DSA:3.30.930.10coord: 178..248
score: 1.1E-25coord: 151..177
score: 6.
NoneNo IPR availableunknownSSF55681Class II aaRS and biotin synthetasescoord: 175..248
score: 8.64

The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
CmoCh16G003190CmoCh04G004580Cucurbita moschata (Rifu)cmocmoB286
The following block(s) are covering this gene:
GeneOrganismBlock
CmoCh16G003190Cucumber (Chinese Long) v3cmocucB0409
CmoCh16G003190Cucurbita moschata (Rifu)cmocmoB096
CmoCh16G003190Cucumber (Gy14) v1cgycmoB0625
CmoCh16G003190Cucurbita maxima (Rimu)cmacmoB133
CmoCh16G003190Cucumber (Chinese Long) v2cmocuB338
CmoCh16G003190Cucurbita pepo (Zucchini)cmocpeB321
CmoCh16G003190Melon (DHL92) v3.6.1cmomedB317