CmoCh15G006730 (gene) Cucurbita moschata (Rifu)

NameCmoCh15G006730
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu))
DescriptionRING/U-box superfamily protein, putative
LocationCmo_Chr15 : 3289192 .. 3290120 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TACGCCAACTGAAGTAAAAGAACGAATTTTAAAGGGACGCATGGATTGATATTTCTTCGGATGTTTGAAAGATGACGTGTTTCATGATTACTTCCAATTAGGCTGCAGCCCAGGGCAACAAAGTTCGTGAGTGAAATGCAATAGACTTTCATGCTAGGCTGGAACTTGTAATGTGCTCTATATATATTTGACATGGAGTTGGCTTTATCTAGAACACACATGAGACAATTTTTATGAAACGAAATGTGTGCCTTTTGGTTCCAAAAATGATCAATTTAGATTCCAAAATTTCCAGCTCAAATATATTTACTCGTAGTCAATTATATTCTGTCTTGTCCATCTCTTGAATCAGTTCTTGTGGAACCGCCAAGAGGTAAGGTAAGAAATGGCTTGATGTTTTGCCAAAATGACAACTTTCCATTTCTTTGATCAAAACCTGCCTTCTCTGCCAAGAAAACTTATGGCCCCTAGAATATTGAAATGACCATGAGAGTTGGTTTCTTGTTGCCTTCTATGAAAGTTGTCTCGCAATGAAGAAACCAGCAAAATATGCAACCTCATATATCCTAATCCATGGCTCATGTTGCACTGTTCTCAATGATTTTTATCCTTTGTAAATGTTGGAACAGGTGAACTCAGATTTGATCATGGCCTTGGCAATGCAGCAGCAGGAGCATGAGATGCCCTATACGACGCTTGGGACCATCGAAAGTGATAGCGACGAAGATGAAAGGTCTGATTCCAATAGCAACAATGGTCTCGACACCAATGCCTCCTTCCAAGTTGAATATGAGGATAGTGAAGAATTGGCTGCCCTTCCTTGTGAGCATCCTTACCATTCAGAGTGTATAGTAAAATGGGTTCAAATCAAAAGGGTTTGTCCAATATGTGGCACAGAAGTTTCATCCCCCAAGCCTTCTAAAAATGCA

mRNA sequence

TACGCCAACTGAAGTGAACTCAGATTTGATCATGGCCTTGGCAATGCAGCAGCAGGAGCATGAGATGCCCTATACGACGCTTGGGACCATCGAAAGTGATAGCGACGAAGATGAAAGGTCTGATTCCAATAGCAACAATGGTCTCGACACCAATGCCTCCTTCCAAGTTGAATATGAGGATAGTGAAGAATTGGCTGCCCTTCCTTGTGAGCATCCTTACCATTCAGAGTGTATAGTAAAATGGGTTCAAATCAAAAGGGTTTGTCCAATATGTGGCACAGAAGTTTCATCCCCCAAGCCTTCTAAAAATGCA

Coding sequence (CDS)

ATGGCCTTGGCAATGCAGCAGCAGGAGCATGAGATGCCCTATACGACGCTTGGGACCATCGAAAGTGATAGCGACGAAGATGAAAGGTCTGATTCCAATAGCAACAATGGTCTCGACACCAATGCCTCCTTCCAAGTTGAATATGAGGATAGTGAAGAATTGGCTGCCCTTCCTTGTGAGCATCCTTACCATTCAGAGTGTATAGTAAAATGGGTTCAAATCAAAAGGGTTTGTCCAATATGTGGCACAGAAGTTTCATCCCCCAAGCCTTCTAAAAATGCA
BLAST of CmoCh15G006730 vs. Swiss-Prot
Match: BBR_ARATH (E3 ubiquitin ligase BIG BROTHER-related OS=Arabidopsis thaliana GN=BBR PE=2 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 1.4e-09
Identity = 29/69 (42.03%), Postives = 43/69 (62.32%), Query Frame = 1

Query: 19  TIESDSDEDERSDSNSNNGLDTNASFQVEYEDSEELAALPCEHPYHSECIVKWVQIKRVC 78
           TI S   +  +   N N   ++    +++YED E+L  LPC+H YHSECI  W++I +VC
Sbjct: 266 TIASLPSKRYKEGDNQNGTNESCVICRLDYEDDEDLILLPCKHSYHSECINNWLKINKVC 325

Query: 79  PICGTEVSS 88
           P+C  EVS+
Sbjct: 326 PVCSAEVST 334

BLAST of CmoCh15G006730 vs. Swiss-Prot
Match: BB_ARATH (E3 ubiquitin ligase BIG BROTHER OS=Arabidopsis thaliana GN=BB PE=1 SV=1)

HSP 1 Score: 58.2 bits (139), Expect = 5.8e-08
Identity = 22/41 (53.66%), Postives = 30/41 (73.17%), Query Frame = 1

Query: 45  QVEYEDSEELAALPCEHPYHSECIVKWVQIKRVCPICGTEV 86
           Q++Y+  E    LPC+H YHSECI KW+ I +VCP+C +EV
Sbjct: 201 QLKYKIGERQMNLPCKHVYHSECISKWLSINKVCPVCNSEV 241

BLAST of CmoCh15G006730 vs. Swiss-Prot
Match: RN122_HUMAN (RING finger protein 122 OS=Homo sapiens GN=RNF122 PE=2 SV=2)

HSP 1 Score: 56.6 bits (135), Expect = 1.7e-07
Identity = 17/42 (40.48%), Postives = 32/42 (76.19%), Query Frame = 1

Query: 47  EYEDSEELAALPCEHPYHSECIVKWVQIKRVCPICGTEVSSP 89
           +++  +EL  LPC+H +H +C+VKW++++ VCP+C   ++SP
Sbjct: 99  DFKGKDELGVLPCQHAFHRKCLVKWLEVRCVCPMCNKPIASP 140

BLAST of CmoCh15G006730 vs. Swiss-Prot
Match: RIE1_ARATH (E3 ubiquitin protein ligase RIE1 OS=Arabidopsis thaliana GN=RIE1 PE=2 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 3.8e-07
Identity = 20/34 (58.82%), Postives = 26/34 (76.47%), Query Frame = 1

Query: 48  YEDSEELAALPCEHPYHSECIVKWVQIKRVCPIC 82
           YED  EL ALPC H +HS CIVKW++++  CP+C
Sbjct: 314 YEDGAELHALPCNHHFHSTCIVKWLKMRATCPLC 347

BLAST of CmoCh15G006730 vs. Swiss-Prot
Match: RN122_MOUSE (RING finger protein 122 OS=Mus musculus GN=Rnf122 PE=2 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 4.9e-07
Identity = 16/42 (38.10%), Postives = 31/42 (73.81%), Query Frame = 1

Query: 47  EYEDSEELAALPCEHPYHSECIVKWVQIKRVCPICGTEVSSP 89
           +++  +EL  LPC+H +H +C+VKW++++ VCP+C   ++ P
Sbjct: 99  DFKGKDELGVLPCQHAFHRKCLVKWLEVRCVCPMCNKPIAGP 140

BLAST of CmoCh15G006730 vs. TrEMBL
Match: A0A0A0KFS4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G382920 PE=4 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 7.3e-18
Identity = 44/60 (73.33%), Postives = 50/60 (83.33%), Query Frame = 1

Query: 34  SNNGLDTNASFQVEYEDSEELAALPCEHPYHSECIVKWVQIKRVCPICGTEVSSPKPSKN 93
           + +G+D     QVEY+D EELAALPCEHPYHSECI +W+QIKRVCPICGTEVSSP  SKN
Sbjct: 157 NRSGIDRCVICQVEYDDGEELAALPCEHPYHSECIGEWLQIKRVCPICGTEVSSPNGSKN 216

BLAST of CmoCh15G006730 vs. TrEMBL
Match: A0A061FHG8_THECC (RING/U-box superfamily protein, putative OS=Theobroma cacao GN=TCM_035161 PE=4 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 4.9e-14
Identity = 38/60 (63.33%), Postives = 47/60 (78.33%), Query Frame = 1

Query: 35  NNGLDTNASFQVEYEDSEELAALP-CEHPYHSECIVKWVQIKRVCPICGTEVSSPKPSKN 94
           N G+D  A  Q+EYE+ E + ALP CEHPYHSECI KW+QIKR+CPIC TE+SS K ++N
Sbjct: 145 NTGIDRCAICQMEYEEEEGVVALPNCEHPYHSECISKWLQIKRICPICSTEISSSKNARN 204

BLAST of CmoCh15G006730 vs. TrEMBL
Match: A0A0B2SRX7_GLYSO (E3 ubiquitin ligase BIG BROTHER-related OS=Glycine soja GN=glysoja_018384 PE=4 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 8.4e-14
Identity = 37/62 (59.68%), Postives = 46/62 (74.19%), Query Frame = 1

Query: 32  SNSNNGLDTNASFQVEYEDSEELAALPCEHPYHSECIVKWVQIKRVCPICGTEVSSPKPS 91
           + S +G+D     QVEYE+ E L AL CEHPYHS+CI KW+QIK+VCPICG EVS+P  +
Sbjct: 140 AESKSGIDRCVICQVEYEEGEALVALQCEHPYHSDCIRKWLQIKKVCPICGNEVSTPNLA 199

Query: 92  KN 94
            N
Sbjct: 200 SN 201

BLAST of CmoCh15G006730 vs. TrEMBL
Match: C6T2M3_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_04G249100 PE=2 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 8.4e-14
Identity = 37/62 (59.68%), Postives = 46/62 (74.19%), Query Frame = 1

Query: 32  SNSNNGLDTNASFQVEYEDSEELAALPCEHPYHSECIVKWVQIKRVCPICGTEVSSPKPS 91
           + S +G+D     QVEYE+ E L AL CEHPYHS+CI KW+QIK+VCPICG EVS+P  +
Sbjct: 140 AESKSGIDRCVICQVEYEEGEALVALQCEHPYHSDCIRKWLQIKKVCPICGNEVSTPNLA 199

Query: 92  KN 94
            N
Sbjct: 200 SN 201

BLAST of CmoCh15G006730 vs. TrEMBL
Match: A0A067K9Q5_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_12129 PE=4 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 8.4e-14
Identity = 36/60 (60.00%), Postives = 45/60 (75.00%), Query Frame = 1

Query: 34  SNNGLDTNASFQVEYEDSEELAALPCEHPYHSECIVKWVQIKRVCPICGTEVSSPKPSKN 93
           + +G+D     QVEYE  E L ALPC+HPYHSECI  W+QIK++CPIC +EVSSPK  +N
Sbjct: 149 NKSGIDRCVICQVEYEGDESLVALPCDHPYHSECISNWLQIKKICPICNSEVSSPKIVRN 208

BLAST of CmoCh15G006730 vs. TAIR10
Match: AT3G47180.1 (AT3G47180.1 RING/U-box superfamily protein)

HSP 1 Score: 65.5 bits (158), Expect = 2.0e-11
Identity = 28/56 (50.00%), Postives = 40/56 (71.43%), Query Frame = 1

Query: 32  SNSNNGLDTNASFQVEYEDSEELAAL-PCEHPYHSECIVKWVQIKRVCPICGTEVS 87
           S++ N +D     Q+E+E+ E L  L PC+HPYHSECI KW++ K++CPIC +E S
Sbjct: 153 SHNKNEIDRCVVCQMEFEERESLVVLRPCDHPYHSECITKWLETKKICPICCSEPS 208

BLAST of CmoCh15G006730 vs. TAIR10
Match: AT3G19910.1 (AT3G19910.1 RING/U-box superfamily protein)

HSP 1 Score: 63.5 bits (153), Expect = 7.8e-11
Identity = 29/69 (42.03%), Postives = 43/69 (62.32%), Query Frame = 1

Query: 19  TIESDSDEDERSDSNSNNGLDTNASFQVEYEDSEELAALPCEHPYHSECIVKWVQIKRVC 78
           TI S   +  +   N N   ++    +++YED E+L  LPC+H YHSECI  W++I +VC
Sbjct: 266 TIASLPSKRYKEGDNQNGTNESCVICRLDYEDDEDLILLPCKHSYHSECINNWLKINKVC 325

Query: 79  PICGTEVSS 88
           P+C  EVS+
Sbjct: 326 PVCSAEVST 334

BLAST of CmoCh15G006730 vs. TAIR10
Match: AT3G63530.1 (AT3G63530.1 RING/U-box superfamily protein)

HSP 1 Score: 58.2 bits (139), Expect = 3.3e-09
Identity = 22/41 (53.66%), Postives = 30/41 (73.17%), Query Frame = 1

Query: 45  QVEYEDSEELAALPCEHPYHSECIVKWVQIKRVCPICGTEV 86
           Q++Y+  E    LPC+H YHSECI KW+ I +VCP+C +EV
Sbjct: 201 QLKYKIGERQMNLPCKHVYHSECISKWLSINKVCPVCNSEV 241

BLAST of CmoCh15G006730 vs. TAIR10
Match: AT5G10650.1 (AT5G10650.1 RING/U-box superfamily protein)

HSP 1 Score: 55.5 bits (132), Expect = 2.1e-08
Identity = 20/45 (44.44%), Postives = 28/45 (62.22%), Query Frame = 1

Query: 45  QVEYEDSEELAALPCEHPYHSECIVKWVQIKRVCPICGTEVSSPK 90
           Q EY D +EL  +PC+H YH  C+ +W+++K  CPIC T     K
Sbjct: 479 QEEYVDGDELGTIPCQHMYHVSCVQQWLRMKNWCPICKTSAEEEK 523

BLAST of CmoCh15G006730 vs. TAIR10
Match: AT2G01735.1 (AT2G01735.1 RING-finger protein for embryogenesis)

HSP 1 Score: 55.5 bits (132), Expect = 2.1e-08
Identity = 20/34 (58.82%), Postives = 26/34 (76.47%), Query Frame = 1

Query: 48  YEDSEELAALPCEHPYHSECIVKWVQIKRVCPIC 82
           YED  EL ALPC H +HS CIVKW++++  CP+C
Sbjct: 314 YEDGAELHALPCNHHFHSTCIVKWLKMRATCPLC 347

BLAST of CmoCh15G006730 vs. NCBI nr
Match: gi|449464174|ref|XP_004149804.1| (PREDICTED: E3 ubiquitin ligase BIG BROTHER-related-like [Cucumis sativus])

HSP 1 Score: 97.8 bits (242), Expect = 1.1e-17
Identity = 44/60 (73.33%), Postives = 50/60 (83.33%), Query Frame = 1

Query: 34  SNNGLDTNASFQVEYEDSEELAALPCEHPYHSECIVKWVQIKRVCPICGTEVSSPKPSKN 93
           + +G+D     QVEY+D EELAALPCEHPYHSECI +W+QIKRVCPICGTEVSSP  SKN
Sbjct: 157 NRSGIDRCVICQVEYDDGEELAALPCEHPYHSECIGEWLQIKRVCPICGTEVSSPNGSKN 216

BLAST of CmoCh15G006730 vs. NCBI nr
Match: gi|659133455|ref|XP_008466738.1| (PREDICTED: E3 ubiquitin ligase BIG BROTHER-related-like [Cucumis melo])

HSP 1 Score: 96.7 bits (239), Expect = 2.3e-17
Identity = 43/60 (71.67%), Postives = 50/60 (83.33%), Query Frame = 1

Query: 34  SNNGLDTNASFQVEYEDSEELAALPCEHPYHSECIVKWVQIKRVCPICGTEVSSPKPSKN 93
           + +G+D     QVEY+D EELAALPC+HPYHSECI +W+QIKRVCPICGTEVSSP  SKN
Sbjct: 168 NKSGIDRCVICQVEYDDGEELAALPCQHPYHSECIGEWLQIKRVCPICGTEVSSPNGSKN 227

BLAST of CmoCh15G006730 vs. NCBI nr
Match: gi|720070255|ref|XP_010277675.1| (PREDICTED: E3 ubiquitin ligase BIG BROTHER-related isoform X2 [Nelumbo nucifera])

HSP 1 Score: 88.2 bits (217), Expect = 8.3e-15
Identity = 41/66 (62.12%), Postives = 47/66 (71.21%), Query Frame = 1

Query: 29  RSDSNSNNGLDTNASFQVEYEDSEELAALPCEHPYHSECIVKWVQIKRVCPICGTEVSSP 88
           +  S   NGLD     QVEYE+ E L ALPCEHPYHS+CI KW+QIKRVCPIC TEVS  
Sbjct: 133 KCQSECKNGLDRCVICQVEYEEGESLVALPCEHPYHSDCISKWLQIKRVCPICSTEVSPS 192

Query: 89  KPSKNA 95
           + S +A
Sbjct: 193 EHSSSA 198

BLAST of CmoCh15G006730 vs. NCBI nr
Match: gi|720070251|ref|XP_010277674.1| (PREDICTED: E3 ubiquitin ligase BIG BROTHER-related isoform X1 [Nelumbo nucifera])

HSP 1 Score: 86.7 bits (213), Expect = 2.4e-14
Identity = 42/69 (60.87%), Postives = 48/69 (69.57%), Query Frame = 1

Query: 29  RSDSNSNNGLDTNASF---QVEYEDSEELAALPCEHPYHSECIVKWVQIKRVCPICGTEV 88
           +  S   NGLD N      QVEYE+ E L ALPCEHPYHS+CI KW+QIKRVCPIC TEV
Sbjct: 133 KCQSECKNGLDRNGRCVICQVEYEEGESLVALPCEHPYHSDCISKWLQIKRVCPICSTEV 192

Query: 89  SSPKPSKNA 95
           S  + S +A
Sbjct: 193 SPSEHSSSA 201

BLAST of CmoCh15G006730 vs. NCBI nr
Match: gi|590599262|ref|XP_007019133.1| (RING/U-box superfamily protein, putative [Theobroma cacao])

HSP 1 Score: 85.1 bits (209), Expect = 7.1e-14
Identity = 38/60 (63.33%), Postives = 47/60 (78.33%), Query Frame = 1

Query: 35  NNGLDTNASFQVEYEDSEELAALP-CEHPYHSECIVKWVQIKRVCPICGTEVSSPKPSKN 94
           N G+D  A  Q+EYE+ E + ALP CEHPYHSECI KW+QIKR+CPIC TE+SS K ++N
Sbjct: 145 NTGIDRCAICQMEYEEEEGVVALPNCEHPYHSECISKWLQIKRICPICSTEISSSKNARN 204

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
BBR_ARATH1.4e-0942.03E3 ubiquitin ligase BIG BROTHER-related OS=Arabidopsis thaliana GN=BBR PE=2 SV=1[more]
BB_ARATH5.8e-0853.66E3 ubiquitin ligase BIG BROTHER OS=Arabidopsis thaliana GN=BB PE=1 SV=1[more]
RN122_HUMAN1.7e-0740.48RING finger protein 122 OS=Homo sapiens GN=RNF122 PE=2 SV=2[more]
RIE1_ARATH3.8e-0758.82E3 ubiquitin protein ligase RIE1 OS=Arabidopsis thaliana GN=RIE1 PE=2 SV=1[more]
RN122_MOUSE4.9e-0738.10RING finger protein 122 OS=Mus musculus GN=Rnf122 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KFS4_CUCSA7.3e-1873.33Uncharacterized protein OS=Cucumis sativus GN=Csa_6G382920 PE=4 SV=1[more]
A0A061FHG8_THECC4.9e-1463.33RING/U-box superfamily protein, putative OS=Theobroma cacao GN=TCM_035161 PE=4 S... [more]
A0A0B2SRX7_GLYSO8.4e-1459.68E3 ubiquitin ligase BIG BROTHER-related OS=Glycine soja GN=glysoja_018384 PE=4 S... [more]
C6T2M3_SOYBN8.4e-1459.68Uncharacterized protein OS=Glycine max GN=GLYMA_04G249100 PE=2 SV=1[more]
A0A067K9Q5_JATCU8.4e-1460.00Uncharacterized protein OS=Jatropha curcas GN=JCGZ_12129 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G47180.12.0e-1150.00 RING/U-box superfamily protein[more]
AT3G19910.17.8e-1142.03 RING/U-box superfamily protein[more]
AT3G63530.13.3e-0953.66 RING/U-box superfamily protein[more]
AT5G10650.12.1e-0844.44 RING/U-box superfamily protein[more]
AT2G01735.12.1e-0858.82 RING-finger protein for embryogenesis[more]
Match NameE-valueIdentityDescription
gi|449464174|ref|XP_004149804.1|1.1e-1773.33PREDICTED: E3 ubiquitin ligase BIG BROTHER-related-like [Cucumis sativus][more]
gi|659133455|ref|XP_008466738.1|2.3e-1771.67PREDICTED: E3 ubiquitin ligase BIG BROTHER-related-like [Cucumis melo][more]
gi|720070255|ref|XP_010277675.1|8.3e-1562.12PREDICTED: E3 ubiquitin ligase BIG BROTHER-related isoform X2 [Nelumbo nucifera][more]
gi|720070251|ref|XP_010277674.1|2.4e-1460.87PREDICTED: E3 ubiquitin ligase BIG BROTHER-related isoform X1 [Nelumbo nucifera][more]
gi|590599262|ref|XP_007019133.1|7.1e-1463.33RING/U-box superfamily protein, putative [Theobroma cacao][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR013083Znf_RING/FYVE/PHD
IPR024766Znf_RING_H2
Vocabulary: Molecular Function
TermDefinition
GO:0008270zinc ion binding
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0051603 proteolysis involved in cellular protein catabolic process
biological_process GO:0008152 metabolic process
biological_process GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process
biological_process GO:0016567 protein ubiquitination
biological_process GO:0008150 biological_process
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
cellular_component GO:0005839 proteasome core complex
cellular_component GO:0005575 cellular_component
molecular_function GO:0016874 ligase activity
molecular_function GO:0004298 threonine-type endopeptidase activity
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0005515 protein binding
molecular_function GO:0061630 ubiquitin protein ligase activity
molecular_function GO:0003674 molecular_function

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh15G006730.1CmoCh15G006730.1mRNA


Analysis Name: InterPro Annotations of Cucurbita moschata
Date Performed: 2017-05-19
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3DG3DSA:3.30.40.10coord: 40..89
score: 1.0
IPR024766Zinc finger, RING-H2-typePFAMPF12678zf-rbx1coord: 46..81
score: 4.
NoneNo IPR availablePANTHERPTHR14155RING FINGER DOMAIN-CONTAININGcoord: 45..93
score: 1.0
NoneNo IPR availablePANTHERPTHR14155:SF112SUBFAMILY NOT NAMEDcoord: 45..93
score: 1.0
NoneNo IPR availableunknownSSF57850RING/U-boxcoord: 47..88
score: 1.02

The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
CmoCh15G006730CmoCh04G024890Cucurbita moschata (Rifu)cmocmoB262