CmoCh15G003220 (gene) Cucurbita moschata (Rifu)

Overview
NameCmoCh15G003220
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu))
DescriptionMatrix metalloproteinase
LocationCmo_Chr15 : 1493935 .. 1494616 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCGAAACCTCGATGCGGGGTTGCGGATATCATCGACGGCAAGAGTGGGATGAAATCAGGCAAGAATATGGTGAATCAACATAGAAAAAGTGGTCATTTCCATCAAGTTTCTCATTTTACTTTCTTCAAAGGAAACCTTAAATGGCCTAATTCCAAGTCTCACCTCACTTATGGTAAATTTAGTTTTCATATAAAGTTGAGTTATATTTTACATCATCTTCTATACTAATATCATGATTTATTTAGGATTCCTCCCTGGAACTCCGTCGGGGGCGGTAGATCCGGTGGGTCGAGCTTTTACGACATGGGCTGCGAACACTCACTTCAGCTTCTCCCTAGCATCTAGCTACAAAACTGCTGACATCAAAATAAGCTTTGAAAGGGGTGACCATGGAGATGGCTTTCCCTTTGACAATGTTGGAGGAGTGATAGCCCATGCTTTTGCCCCTACAGATGGGCGGTTACATTTTGACGCAGTCGAGCGTTGGACCGATGGGGCGGTTCCTAATTCGTACGATGTCGAGACGGTAGCATTGCATGAGATCGGACACCTTCTCGGCCTTTATCATAGCTCGGTCGAAGGGGCTATTATGTGGCCAACCATCATGAGAGGAGCTACCAAGGGTTTGCATGCCGATGATATTGCAGGCATCAAGGCTTTATACACTCCTACCTCTTGA

mRNA sequence

ATGTCGAAACCTCGATGCGGGGTTGCGGATATCATCGACGGCAAGAGTGGGATGAAATCAGGCAAGAATATGGTGAATCAACATAGAAAAAGTGGTCATTTCCATCAAGTTTCTCATTTTACTTTCTTCAAAGGAAACCTTAAATGGCCTAATTCCAAGTCTCACCTCACTTATGGATTCCTCCCTGGAACTCCGTCGGGGGCGGTAGATCCGGTGGGTCGAGCTTTTACGACATGGGCTGCGAACACTCACTTCAGCTTCTCCCTAGCATCTAGCTACAAAACTGCTGACATCAAAATAAGCTTTGAAAGGGGTGACCATGGAGATGGCTTTCCCTTTGACAATGTTGGAGGAGTGATAGCCCATGCTTTTGCCCCTACAGATGGGCGGTTACATTTTGACGCAGTCGAGCGTTGGACCGATGGGGCGGTTCCTAATTCGTACGATGTCGAGACGGTAGCATTGCATGAGATCGGACACCTTCTCGGCCTTTATCATAGCTCGGTCGAAGGGGCTATTATGTGGCCAACCATCATGAGAGGAGCTACCAAGGGTTTGCATGCCGATGATATTGCAGGCATCAAGGCTTTATACACTCCTACCTCTTGA

Coding sequence (CDS)

ATGTCGAAACCTCGATGCGGGGTTGCGGATATCATCGACGGCAAGAGTGGGATGAAATCAGGCAAGAATATGGTGAATCAACATAGAAAAAGTGGTCATTTCCATCAAGTTTCTCATTTTACTTTCTTCAAAGGAAACCTTAAATGGCCTAATTCCAAGTCTCACCTCACTTATGGATTCCTCCCTGGAACTCCGTCGGGGGCGGTAGATCCGGTGGGTCGAGCTTTTACGACATGGGCTGCGAACACTCACTTCAGCTTCTCCCTAGCATCTAGCTACAAAACTGCTGACATCAAAATAAGCTTTGAAAGGGGTGACCATGGAGATGGCTTTCCCTTTGACAATGTTGGAGGAGTGATAGCCCATGCTTTTGCCCCTACAGATGGGCGGTTACATTTTGACGCAGTCGAGCGTTGGACCGATGGGGCGGTTCCTAATTCGTACGATGTCGAGACGGTAGCATTGCATGAGATCGGACACCTTCTCGGCCTTTATCATAGCTCGGTCGAAGGGGCTATTATGTGGCCAACCATCATGAGAGGAGCTACCAAGGGTTTGCATGCCGATGATATTGCAGGCATCAAGGCTTTATACACTCCTACCTCTTGA
Homology
[Close]
BLAST of CmoCh15G003220 vs. Swiss-Prot
Match: 2MMP_ARATH (Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana GN=2MMP PE=1 SV=1)

HSP 1 Score: 168.3 bits (425), Expect = 8.5e-41
Identity = 94/206 (45.63%), Postives = 122/206 (59.22%), Query Frame = 1 

Query: 4   PRCGVADIIDGKSGMKSGKNMVNQHRKSG-HFHQVSHFTFFKGNLKWPNSKSHLTYGFLP 63
           PRCG  D+++G S M  G+    +   S  H H V  +T F G  +WP ++  LTY F P
Sbjct: 118 PRCGNPDVVNGTSLMHGGRRKTFEVNFSRTHLHAVKRYTLFPGEPRWPRNRRDLTYAFDP 177

Query: 64  GTP-SGAVDPV-GRAFTTWAANTHFSFSLASSYKTADIKISFERGDHGDGFPFDNVGGVI 123
             P +  V  V  RAF  W+  T  +F+L+ S+ T+DI I F  GDHGDG PFD V G +
Sbjct: 178 KNPLTEEVKSVFSRAFGRWSDVTALNFTLSESFSTSDITIGFYTGDHGDGEPFDGVLGTL 237

Query: 124 AHAFAPTDGRLHFDAVERWTDG-------AVPNSYDVETVALHEIGHLLGLYHSSVEGAI 183
           AHAF+P  G+ H DA E W          +V  + D+E+VA+HEIGHLLGL HSSVE +I
Sbjct: 238 AHAFSPPSGKFHLDADENWVVSGDLDSFLSVTAAVDLESVAVHEIGHLLGLGHSSVEESI 297

Query: 184 MWPTIMRGATK-GLHADDIAGIKALY 199
           M+PTI  G  K  L  DD+ GI+ LY
Sbjct: 298 MYPTITTGKRKVDLTNDDVEGIQYLY 323

[Close]
BLAST of CmoCh15G003220 vs. Swiss-Prot
Match: 3MMP_ARATH (Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana GN=3MMP PE=1 SV=1)

HSP 1 Score: 166.0 bits (419), Expect = 4.2e-40
Identity = 90/208 (43.27%), Postives = 118/208 (56.73%), Query Frame = 1 

Query: 4   PRCGVADIIDGKSGMKSGKNM--VNQHRKSGHFHQVSHFTFFKGNLKWPNSKSHLTYGFL 63
           PRCG  D+++G S M SG+    V+   +   FH V H++FF G  +WP ++  LTY F 
Sbjct: 121 PRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHAVKHYSFFPGEPRWPRNRRDLTYAFD 180

Query: 64  P--GTPSGAVDPVGRAFTTWAANTHFSFSLASSYKTADIKISFERGDHGDGFPFDNVGGV 123
           P             RAFT W   T  +F+    + T+DI I F  G+HGDG PFD     
Sbjct: 181 PRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERFSTSDISIGFYSGEHGDGEPFDGPMRT 240

Query: 124 IAHAFAPTDGRLHFDAVERWT------DG--AVPNSYDVETVALHEIGHLLGLYHSSVEG 183
           +AHAF+P  G  H D  E W       DG  +V  + D+E+VA+HEIGHLLGL HSSVEG
Sbjct: 241 LAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAVDLESVAVHEIGHLLGLGHSSVEG 300

Query: 184 AIMWPTIMRGATK-GLHADDIAGIKALY 199
           +IM+PTI  G  K  L  DD+ G++ LY
Sbjct: 301 SIMYPTIRTGRRKVDLTTDDVEGVQYLY 328

[Close]
BLAST of CmoCh15G003220 vs. Swiss-Prot
Match: MEP1_SOYBN (Metalloendoproteinase 1 OS=Glycine max PE=1 SV=2)

HSP 1 Score: 156.8 bits (395), Expect = 2.6e-37
Identity = 90/204 (44.12%), Postives = 117/204 (57.35%), Query Frame = 1 

Query: 4   PRCGVADIIDGKSGMKSGKNMVNQHRKSGHFHQVSHFTFFKGNLKWPNSKSHLTYGFLPG 63
           PRCGV DII            +N + K+  F  +S +TFFK   +W    + LTY F P 
Sbjct: 111 PRCGVPDII------------INTN-KTTSFGMISDYTFFKDMPRWQAGTTQLTYAFSPE 170

Query: 64  TPSGAV--DPVGRAFTTWAANTHFSFSLASSYKTADIKISFERGDHGDGFPFDNVGGVIA 123
                     + RAF+ W    + +F   +SY+TA+IKI F   +HGD +PFD  GG++ 
Sbjct: 171 PRLDDTFKSAIARAFSKWTPVVNIAFQETTSYETANIKILFASKNHGDPYPFDGPGGILG 230

Query: 124 HAFAPTDGRLHFDAVERW------TDGAVPNSYDVETVALHEIGHLLGLYHSSVEGAIMW 183
           HAFAPTDGR HFDA E W      T   V +++D+E+VA+HEIGHLLGL HSS   AIM+
Sbjct: 231 HAFAPTDGRCHFDADEYWVASGDVTKSPVTSAFDLESVAVHEIGHLLGLGHSSDLRAIMY 290

Query: 184 PTIMRGATK-GLHADDIAGIKALY 199
           P+I     K  L  DDI GI+ LY
Sbjct: 291 PSIPPRTRKVNLAQDDIDGIRKLY 301

[Close]
BLAST of CmoCh15G003220 vs. Swiss-Prot
Match: 5MMP_ARATH (Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana GN=5MMP PE=1 SV=1)

HSP 1 Score: 152.5 bits (384), Expect = 4.8e-36
Identity = 94/210 (44.76%), Postives = 114/210 (54.29%), Query Frame = 1 

Query: 3   KPRCGVADIIDGKSGMKSGKNMVNQHRKSGHFHQVSHFTFFKGNLKWPNSKSHLTYGFLP 62
           KPRCG  D+IDG S M  GK +    +          ++FF G  +WP  K  LTY F P
Sbjct: 116 KPRCGNPDLIDGVSEMNGGKILRTTEK----------YSFFPGKPRWPKRKRDLTYAFAP 175

Query: 63  GTPSGAVDPV----GRAFTTWAANTHFSFSLASSYKTADIKISFERGDHGDGFPFDNVGG 122
              +   D V     RAFT WA  T  +F+ + S   ADI I F  G+HGDG PFD   G
Sbjct: 176 --QNNLTDEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEPFDGAMG 235

Query: 123 VIAHAFAPTDGRLHFDAVERW--TDG-------AVPNSYDVETVALHEIGHLLGLYHSSV 182
            +AHA +P  G LH D  E W  ++G        V    D+E+VA+HEIGHLLGL HSSV
Sbjct: 236 TLAHASSPPTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGLGHSSV 295

Query: 183 EGAIMWPTIMRGATK-GLHADDIAGIKALY 199
           E AIM+P I  G  K  L  DDI GI+ LY
Sbjct: 296 EDAIMFPAISGGDRKVELAKDDIEGIQHLY 313

[Close]
BLAST of CmoCh15G003220 vs. Swiss-Prot
Match: 1MMP_ARATH (Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana GN=1MMP PE=1 SV=1)

HSP 1 Score: 148.7 bits (374), Expect = 7.0e-35
Identity = 89/212 (41.98%), Postives = 114/212 (53.77%), Query Frame = 1 

Query: 1   MSKPRCGVADIIDGKSGMKSGKNMVNQHRKSGHFHQVSHFTFFKGNLKWPNSKSHLTYGF 60
           MS PRCGV+D               +    +   H  +H+T+F G  KW  ++  LTY  
Sbjct: 125 MSLPRCGVSD--------------THMTINNDFLHTTAHYTYFNGKPKW--NRDTLTYAI 184

Query: 61  -----LPGTPSGAVDPV-GRAFTTWAANTHFSFSLASSYKTADIKISFERGDHGDGFPFD 120
                L    S  V  V  RAF+ W++    SF     + TAD+KI F  GDHGDG PFD
Sbjct: 185 SKTHKLDYLTSEDVKTVFRRAFSQWSSVIPVSFEEVDDFTTADLKIGFYAGDHGDGLPFD 244

Query: 121 NVGGVIAHAFAPTDGRLHFDAVERW-----TDGAVPNSYDVETVALHEIGHLLGLYHSSV 180
            V G +AHAFAP +GRLH DA E W       G+   + D+E+VA HEIGHLLGL HSS 
Sbjct: 245 GVLGTLAHAFAPENGRLHLDAAETWIVDDDLKGSSEVAVDLESVATHEIGHLLGLGHSSQ 304

Query: 181 EGAIMWPTIMRGATK-GLHADDIAGIKALYTP 201
           E A+M+P++     K  L  DD+AG+  LY P
Sbjct: 305 ESAVMYPSLRPRTKKVDLTVDDVAGVLKLYGP 320

[Close]
BLAST of CmoCh15G003220 vs. TrEMBL
Match: A0A0A0KS47_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G633160 PE=3 SV=1)

HSP 1 Score: 360.9 bits (925), Expect = 1.0e-96
Identity = 168/200 (84.00%), Postives = 182/200 (91.00%), Query Frame = 1 

Query: 1   MSKPRCGVADIIDGKSGMKSGKNMVNQHRK-SGHFHQVSHFTFFKGNLKWPNSKSHLTYG 60
           MSKPRCGVADII+GK+ MKSGK  VNQHRK SGHFH+VSHF FF+GNL+WP+SKSHLTYG
Sbjct: 112 MSKPRCGVADIINGKTSMKSGKRFVNQHRKISGHFHEVSHFAFFEGNLRWPDSKSHLTYG 171

Query: 61  FLPGTPSGAVDPVGRAFTTWAANTHFSFSLASSYKTADIKISFERGDHGDGFPFDNVGGV 120
           FLPGTPS A+ PV RAFTTWAANTHFSFS  S Y+ ADIKISFE+GDHGDGFPFD+VGGV
Sbjct: 172 FLPGTPSEAISPVNRAFTTWAANTHFSFSQESKYENADIKISFEKGDHGDGFPFDSVGGV 231

Query: 121 IAHAFAPTDGRLHFDAVERWTDGAVPNSYDVETVALHEIGHLLGLYHSSVEGAIMWPTIM 180
           +AHAFAPTDGRLHFDAVE W DGAVP SYD+ETVALHEIGHLLGL+HSSVEGAIMWP+IM
Sbjct: 232 LAHAFAPTDGRLHFDAVEHWADGAVPKSYDMETVALHEIGHLLGLHHSSVEGAIMWPSIM 291

Query: 181 RGATKGLHADDIAGIKALYT 200
            GATKGLHADDI GIK LYT
Sbjct: 292 GGATKGLHADDIEGIKVLYT 311

[Close]
BLAST of CmoCh15G003220 vs. TrEMBL
Match: A0A0A0KU08_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G632130 PE=3 SV=1)

HSP 1 Score: 318.9 bits (816), Expect = 4.4e-84
Identity = 150/199 (75.38%), Postives = 165/199 (82.91%), Query Frame = 1 

Query: 1   MSKPRCGVADIIDGKSGMKSGKNMVNQHRK-SGHFHQVSHFTFFKGNLKWPNSKSHLTYG 60
           MS+PRCG ADI+DGK+ MKSGK MVNQHRK +GHFH VSH+ FF GN KWP SKSHLTYG
Sbjct: 115 MSRPRCGFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYG 174

Query: 61  FLPGTPSGAVDPVGRAFTTWAANTHFSFSLASSYKTADIKISFERGDHGDGFPFDNVGGV 120
           FLPGTPS AV  +GRAF TW ANTHF+FS  S YK ADIKISFE GDHGDG  FD V GV
Sbjct: 175 FLPGTPSKAVATIGRAFKTWGANTHFNFSQTSRYKKADIKISFETGDHGDGHSFDGVRGV 234

Query: 121 IAHAFAPTDGRLHFDAVERWTDGAVPNSYDVETVALHEIGHLLGLYHSSVEGAIMWPTIM 180
           IAH FAP DGRLHFDA E W  GA+ +S+D+ET+ALHEIGHLLGLYHSS+E AIMWP+IM
Sbjct: 235 IAHGFAPRDGRLHFDAAESWAVGAIADSFDLETIALHEIGHLLGLYHSSIERAIMWPSIM 294

Query: 181 RGATKGLHADDIAGIKALY 199
            G+TKGL ADDIAGI ALY
Sbjct: 295 EGSTKGLDADDIAGINALY 313

[Close]
BLAST of CmoCh15G003220 vs. TrEMBL
Match: A0A0A0KS39_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G632120 PE=3 SV=1)

HSP 1 Score: 293.5 bits (750), Expect = 2.0e-76
Identity = 138/199 (69.35%), Postives = 156/199 (78.39%), Query Frame = 1 

Query: 1   MSKPRCGVADIIDGKSGMKSGKNMVN-QHRKSGHFHQVSHFTFFKGNLKWPNSKSHLTYG 60
           MSKPRCGVADII G + M+S K     +H   GHFH VSHF FF GN KWP +KSHLTYG
Sbjct: 117 MSKPRCGVADIIHGNTWMRSSKKRKQLEHGIVGHFHSVSHFAFFDGNPKWPATKSHLTYG 176

Query: 61  FLPGTPSGAVDPVGRAFTTWAANTHFSFSLASSYKTADIKISFERGDHGDGFPFDNVGGV 120
           FLPGTPS  V PV RAF TWAAN+HF+FS A   +T+DI I FE GDHGDG+PFD VGGV
Sbjct: 177 FLPGTPSETVSPVARAFATWAANSHFTFSQALDNQTSDITIGFETGDHGDGYPFDGVGGV 236

Query: 121 IAHAFAPTDGRLHFDAVERWTDGAVPNSYDVETVALHEIGHLLGLYHSSVEGAIMWPTIM 180
           +AHAF+P+DGR H DA E W +G V +S+D+ETVALHEIGHLLGL HSS EGAIMWP + 
Sbjct: 237 VAHAFSPSDGRFHLDADESWANGVVSDSFDLETVALHEIGHLLGLQHSSTEGAIMWPQVP 296

Query: 181 RGATKGLHADDIAGIKALY 199
            GA+KGLH DDIAGIKALY
Sbjct: 297 VGASKGLHVDDIAGIKALY 315

[Close]
BLAST of CmoCh15G003220 vs. TrEMBL
Match: Q9LEL9_CUCSA (Matrix metalloproteinase OS=Cucumis sativus GN=MMP-1 PE=2 SV=1)

HSP 1 Score: 273.5 bits (698), Expect = 2.1e-70
Identity = 126/198 (63.64%), Postives = 151/198 (76.26%), Query Frame = 1 

Query: 1   MSKPRCGVADIIDGKSGMKSGKNMVNQHRKSGHFHQVSHFTFFKGNLKWPNSKSHLTYGF 60
           ++ PRCGV D+I  K   K  +N  N      HFH+VSHFTFF+GNLKWP+SK HL+YGF
Sbjct: 123 IAMPRCGVQDVIKNKKTKKRNQNFTNNGHT--HFHKVSHFTFFEGNLKWPSSKLHLSYGF 182

Query: 61  LPGTPSGAVDPVGRAFTTWAANTHFSFSLASSYKTADIKISFERGDHGDGFPFDNVGGVI 120
           LP  P  A+ PV RAF+ W+ NTHF FS  + Y+ ADIKISFERG+HGD  PFD VGGV+
Sbjct: 183 LPNYPIDAIKPVSRAFSKWSLNTHFKFSHVADYRKADIKISFERGEHGDNAPFDGVGGVL 242

Query: 121 AHAFAPTDGRLHFDAVERWTDGAVPNSYDVETVALHEIGHLLGLYHSSVEGAIMWPTIMR 180
           AHA+APTDGRLHFD  + W+ GA+   +DVETVALHEIGH+LGL HS++E AIM+P+I  
Sbjct: 243 AHAYAPTDGRLHFDGDDAWSVGAISGYFDVETVALHEIGHILGLQHSTIEEAIMFPSIPE 302

Query: 181 GATKGLHADDIAGIKALY 199
           G TKGLH DDIAGIKALY
Sbjct: 303 GVTKGLHGDDIAGIKALY 318

[Close]
BLAST of CmoCh15G003220 vs. TrEMBL
Match: B9RUG6_RICCO (Metalloendoproteinase 1, putative OS=Ricinus communis GN=RCOM_0852690 PE=3 SV=1)

HSP 1 Score: 271.2 bits (692), Expect = 1.0e-69
Identity = 129/198 (65.15%), Postives = 147/198 (74.24%), Query Frame = 1 

Query: 1   MSKPRCGVADIIDGKSGMKSGKNMVNQHRKSGHFHQVSHFTFFKGNLKWPNSKSHLTYGF 60
           M  PRCGVADII+G + M+S  N    H  S  FH VSH+ FF GN +WP SK HLTYGF
Sbjct: 113 MMMPRCGVADIINGTTRMQSS-NKNPHHHSSTSFHTVSHYEFFPGNPRWPASKYHLTYGF 172

Query: 61  LPGTPSGAVDPVGRAFTTWAANTHFSFSLASSYKTADIKISFERGDHGDGFPFDNVGGVI 120
           LPGTP+ A++PV +AF TWAANTHF F+    Y+ ADI I F RGDHGDG PFD  GG +
Sbjct: 173 LPGTPNQAMEPVAKAFQTWAANTHFRFTRVQDYRAADITIGFHRGDHGDGSPFDGRGGTL 232

Query: 121 AHAFAPTDGRLHFDAVERWTDGAVPNSYDVETVALHEIGHLLGLYHSSVEGAIMWPTIMR 180
           AHAFAP DGR H+D  E W  GA   ++DVETVALHEIGHLLGL HSSVEGAIM P+I  
Sbjct: 233 AHAFAPQDGRFHYDGDEHWAVGATQGAFDVETVALHEIGHLLGLGHSSVEGAIMHPSIQS 292

Query: 181 GATKGLHADDIAGIKALY 199
           GATKGLH+DDI GI+ALY
Sbjct: 293 GATKGLHSDDIQGIRALY 309

[Close]
BLAST of CmoCh15G003220 vs. TAIR10
Match: AT1G70170.1 (AT1G70170.1 matrix metalloproteinase)

HSP 1 Score: 168.3 bits (425), Expect = 4.8e-42
Identity = 94/206 (45.63%), Postives = 122/206 (59.22%), Query Frame = 1 

Query: 4   PRCGVADIIDGKSGMKSGKNMVNQHRKSG-HFHQVSHFTFFKGNLKWPNSKSHLTYGFLP 63
           PRCG  D+++G S M  G+    +   S  H H V  +T F G  +WP ++  LTY F P
Sbjct: 118 PRCGNPDVVNGTSLMHGGRRKTFEVNFSRTHLHAVKRYTLFPGEPRWPRNRRDLTYAFDP 177

Query: 64  GTP-SGAVDPV-GRAFTTWAANTHFSFSLASSYKTADIKISFERGDHGDGFPFDNVGGVI 123
             P +  V  V  RAF  W+  T  +F+L+ S+ T+DI I F  GDHGDG PFD V G +
Sbjct: 178 KNPLTEEVKSVFSRAFGRWSDVTALNFTLSESFSTSDITIGFYTGDHGDGEPFDGVLGTL 237

Query: 124 AHAFAPTDGRLHFDAVERWTDG-------AVPNSYDVETVALHEIGHLLGLYHSSVEGAI 183
           AHAF+P  G+ H DA E W          +V  + D+E+VA+HEIGHLLGL HSSVE +I
Sbjct: 238 AHAFSPPSGKFHLDADENWVVSGDLDSFLSVTAAVDLESVAVHEIGHLLGLGHSSVEESI 297

Query: 184 MWPTIMRGATK-GLHADDIAGIKALY 199
           M+PTI  G  K  L  DD+ GI+ LY
Sbjct: 298 MYPTITTGKRKVDLTNDDVEGIQYLY 323

[Close]
BLAST of CmoCh15G003220 vs. TAIR10
Match: AT1G24140.1 (AT1G24140.1 Matrixin family protein)

HSP 1 Score: 166.0 bits (419), Expect = 2.4e-41
Identity = 90/208 (43.27%), Postives = 118/208 (56.73%), Query Frame = 1 

Query: 4   PRCGVADIIDGKSGMKSGKNM--VNQHRKSGHFHQVSHFTFFKGNLKWPNSKSHLTYGFL 63
           PRCG  D+++G S M SG+    V+   +   FH V H++FF G  +WP ++  LTY F 
Sbjct: 121 PRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHAVKHYSFFPGEPRWPRNRRDLTYAFD 180

Query: 64  P--GTPSGAVDPVGRAFTTWAANTHFSFSLASSYKTADIKISFERGDHGDGFPFDNVGGV 123
           P             RAFT W   T  +F+    + T+DI I F  G+HGDG PFD     
Sbjct: 181 PRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERFSTSDISIGFYSGEHGDGEPFDGPMRT 240

Query: 124 IAHAFAPTDGRLHFDAVERWT------DG--AVPNSYDVETVALHEIGHLLGLYHSSVEG 183
           +AHAF+P  G  H D  E W       DG  +V  + D+E+VA+HEIGHLLGL HSSVEG
Sbjct: 241 LAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAVDLESVAVHEIGHLLGLGHSSVEG 300

Query: 184 AIMWPTIMRGATK-GLHADDIAGIKALY 199
           +IM+PTI  G  K  L  DD+ G++ LY
Sbjct: 301 SIMYPTIRTGRRKVDLTTDDVEGVQYLY 328

[Close]
BLAST of CmoCh15G003220 vs. TAIR10
Match: AT1G59970.1 (AT1G59970.1 Matrixin family protein)

HSP 1 Score: 152.5 bits (384), Expect = 2.7e-37
Identity = 94/210 (44.76%), Postives = 114/210 (54.29%), Query Frame = 1 

Query: 3   KPRCGVADIIDGKSGMKSGKNMVNQHRKSGHFHQVSHFTFFKGNLKWPNSKSHLTYGFLP 62
           KPRCG  D+IDG S M  GK +    +          ++FF G  +WP  K  LTY F P
Sbjct: 116 KPRCGNPDLIDGVSEMNGGKILRTTEK----------YSFFPGKPRWPKRKRDLTYAFAP 175

Query: 63  GTPSGAVDPV----GRAFTTWAANTHFSFSLASSYKTADIKISFERGDHGDGFPFDNVGG 122
              +   D V     RAFT WA  T  +F+ + S   ADI I F  G+HGDG PFD   G
Sbjct: 176 --QNNLTDEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEPFDGAMG 235

Query: 123 VIAHAFAPTDGRLHFDAVERW--TDG-------AVPNSYDVETVALHEIGHLLGLYHSSV 182
            +AHA +P  G LH D  E W  ++G        V    D+E+VA+HEIGHLLGL HSSV
Sbjct: 236 TLAHASSPPTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGLGHSSV 295

Query: 183 EGAIMWPTIMRGATK-GLHADDIAGIKALY 199
           E AIM+P I  G  K  L  DDI GI+ LY
Sbjct: 296 EDAIMFPAISGGDRKVELAKDDIEGIQHLY 313

[Close]
BLAST of CmoCh15G003220 vs. TAIR10
Match: AT4G16640.1 (AT4G16640.1 Matrixin family protein)

HSP 1 Score: 148.7 bits (374), Expect = 3.9e-36
Identity = 89/212 (41.98%), Postives = 114/212 (53.77%), Query Frame = 1 

Query: 1   MSKPRCGVADIIDGKSGMKSGKNMVNQHRKSGHFHQVSHFTFFKGNLKWPNSKSHLTYGF 60
           MS PRCGV+D               +    +   H  +H+T+F G  KW  ++  LTY  
Sbjct: 125 MSLPRCGVSD--------------THMTINNDFLHTTAHYTYFNGKPKW--NRDTLTYAI 184

Query: 61  -----LPGTPSGAVDPV-GRAFTTWAANTHFSFSLASSYKTADIKISFERGDHGDGFPFD 120
                L    S  V  V  RAF+ W++    SF     + TAD+KI F  GDHGDG PFD
Sbjct: 185 SKTHKLDYLTSEDVKTVFRRAFSQWSSVIPVSFEEVDDFTTADLKIGFYAGDHGDGLPFD 244

Query: 121 NVGGVIAHAFAPTDGRLHFDAVERW-----TDGAVPNSYDVETVALHEIGHLLGLYHSSV 180
            V G +AHAFAP +GRLH DA E W       G+   + D+E+VA HEIGHLLGL HSS 
Sbjct: 245 GVLGTLAHAFAPENGRLHLDAAETWIVDDDLKGSSEVAVDLESVATHEIGHLLGLGHSSQ 304

Query: 181 EGAIMWPTIMRGATK-GLHADDIAGIKALYTP 201
           E A+M+P++     K  L  DD+AG+  LY P
Sbjct: 305 ESAVMYPSLRPRTKKVDLTVDDVAGVLKLYGP 320

[Close]
BLAST of CmoCh15G003220 vs. TAIR10
Match: AT2G45040.1 (AT2G45040.1 Matrixin family protein)

HSP 1 Score: 135.2 bits (339), Expect = 4.5e-32
Identity = 80/207 (38.65%), Postives = 106/207 (51.21%), Query Frame = 1 

Query: 4   PRCGVADIIDGKSGMKSGKNMVNQHRKSGHFHQVSHFTFFKGNLKWPNSKS-HLTYGFLP 63
           PRCG  D ++ K+                 FH    + +F G  +W       LTY F  
Sbjct: 104 PRCGFPDDVEPKTAP---------------FHTGKKYVYFPGRPRWTRDVPLKLTYAFSQ 163

Query: 64  GTPSGAVDPVG------RAFTTWAANTHFSFSLASSYKTADIKISFERGDHGDGFPFDNV 123
              +  + P        RAF  WA+    SF     Y  ADIKI F  GDHGDG PFD V
Sbjct: 164 ENLTPYLAPTDIRRVFRRAFGKWASVIPVSFIETEDYVIADIKIGFFNGDHGDGEPFDGV 223

Query: 124 GGVIAHAFAPTDGRLHFDAVERWT----DGAVPNSYDVETVALHEIGHLLGLYHSSVEGA 183
            GV+AH F+P +GRLH D  E W     +     + D+E+VA+HEIGH+LGL HSSV+ A
Sbjct: 224 LGVLAHTFSPENGRLHLDKAETWAVDFDEEKSSVAVDLESVAVHEIGHVLGLGHSSVKDA 283

Query: 184 IMWPTIM-RGATKGLHADDIAGIKALY 199
            M+PT+  R     L+ DD+ G+++LY
Sbjct: 284 AMYPTLKPRSKKVNLNMDDVVGVQSLY 295

[Close]
BLAST of CmoCh15G003220 vs. NCBI nr
Match: gi|659117646|ref|XP_008458713.1| (PREDICTED: metalloendoproteinase 1-like [Cucumis melo])

HSP 1 Score: 362.8 bits (930), Expect = 3.8e-97
Identity = 167/200 (83.50%), Postives = 184/200 (92.00%), Query Frame = 1 

Query: 1   MSKPRCGVADIIDGKSGMKSGKNMVNQHRKS-GHFHQVSHFTFFKGNLKWPNSKSHLTYG 60
           MSKPRCGVADII+G++GMKSGK +VN+HRK  GHFH+VSHF FF+GNLKWPNSKSHLTYG
Sbjct: 81  MSKPRCGVADIINGRTGMKSGKRLVNRHRKIIGHFHEVSHFAFFEGNLKWPNSKSHLTYG 140

Query: 61  FLPGTPSGAVDPVGRAFTTWAANTHFSFSLASSYKTADIKISFERGDHGDGFPFDNVGGV 120
           FLPGTPS A+ PV RAFTTWAANTHFSFS  S+Y+ ADIK++FERGDHGDGFPFD+VGGV
Sbjct: 141 FLPGTPSEAISPVNRAFTTWAANTHFSFSQESNYENADIKVTFERGDHGDGFPFDSVGGV 200

Query: 121 IAHAFAPTDGRLHFDAVERWTDGAVPNSYDVETVALHEIGHLLGLYHSSVEGAIMWPTIM 180
           +AHAFAPTDGRLHFDAVE W DGAVPNSYD+ETVALHEIGHLLGL+HSSVEGAIMWP+IM
Sbjct: 201 LAHAFAPTDGRLHFDAVEHWADGAVPNSYDMETVALHEIGHLLGLHHSSVEGAIMWPSIM 260

Query: 181 RGATKGLHADDIAGIKALYT 200
            G TKGLHADDI GIK LYT
Sbjct: 261 GGTTKGLHADDIEGIKVLYT 280

[Close]
BLAST of CmoCh15G003220 vs. NCBI nr
Match: gi|449449409|ref|XP_004142457.1| (PREDICTED: metalloendoproteinase 1-like [Cucumis sativus])

HSP 1 Score: 360.9 bits (925), Expect = 1.4e-96
Identity = 168/200 (84.00%), Postives = 182/200 (91.00%), Query Frame = 1 

Query: 1   MSKPRCGVADIIDGKSGMKSGKNMVNQHRK-SGHFHQVSHFTFFKGNLKWPNSKSHLTYG 60
           MSKPRCGVADII+GK+ MKSGK  VNQHRK SGHFH+VSHF FF+GNL+WP+SKSHLTYG
Sbjct: 112 MSKPRCGVADIINGKTSMKSGKRFVNQHRKISGHFHEVSHFAFFEGNLRWPDSKSHLTYG 171

Query: 61  FLPGTPSGAVDPVGRAFTTWAANTHFSFSLASSYKTADIKISFERGDHGDGFPFDNVGGV 120
           FLPGTPS A+ PV RAFTTWAANTHFSFS  S Y+ ADIKISFE+GDHGDGFPFD+VGGV
Sbjct: 172 FLPGTPSEAISPVNRAFTTWAANTHFSFSQESKYENADIKISFEKGDHGDGFPFDSVGGV 231

Query: 121 IAHAFAPTDGRLHFDAVERWTDGAVPNSYDVETVALHEIGHLLGLYHSSVEGAIMWPTIM 180
           +AHAFAPTDGRLHFDAVE W DGAVP SYD+ETVALHEIGHLLGL+HSSVEGAIMWP+IM
Sbjct: 232 LAHAFAPTDGRLHFDAVEHWADGAVPKSYDMETVALHEIGHLLGLHHSSVEGAIMWPSIM 291

Query: 181 RGATKGLHADDIAGIKALYT 200
            GATKGLHADDI GIK LYT
Sbjct: 292 GGATKGLHADDIEGIKVLYT 311

[Close]
BLAST of CmoCh15G003220 vs. NCBI nr
Match: gi|659118019|ref|XP_008458907.1| (PREDICTED: metalloendoproteinase 1-like [Cucumis melo])

HSP 1 Score: 329.7 bits (844), Expect = 3.5e-87
Identity = 158/199 (79.40%), Postives = 169/199 (84.92%), Query Frame = 1 

Query: 1   MSKPRCGVADIIDGKSGMKSGKNMVNQHRK-SGHFHQVSHFTFFKGNLKWPNSKSHLTYG 60
           MSKPRCGVADIIDGK+ MKSGK MVNQHRK +GHFH VSH+ FF GN KWP SK HLTYG
Sbjct: 115 MSKPRCGVADIIDGKTRMKSGKKMVNQHRKINGHFHTVSHYAFFDGNPKWPASKFHLTYG 174

Query: 61  FLPGTPSGAVDPVGRAFTTWAANTHFSFSLASSYKTADIKISFERGDHGDGFPFDNVGGV 120
           FLPGTPS AV PVGRAF TW A+THF+FS AS YK ADIKISF  GDHGDG  FD VGGV
Sbjct: 175 FLPGTPSKAVAPVGRAFKTWGAHTHFNFSRASKYKKADIKISFRSGDHGDGHSFDGVGGV 234

Query: 121 IAHAFAPTDGRLHFDAVERWTDGAVPNSYDVETVALHEIGHLLGLYHSSVEGAIMWPTIM 180
           IAH FAPTDGRLHFDA+E W  GAV +S+D+ETVALHEIGHLLGL+HSSVEGAIMWP+IM
Sbjct: 235 IAHGFAPTDGRLHFDAIESWAVGAVADSFDLETVALHEIGHLLGLHHSSVEGAIMWPSIM 294

Query: 181 RGATKGLHADDIAGIKALY 199
            G TKGL ADDIAGI ALY
Sbjct: 295 EGTTKGLDADDIAGINALY 313

[Close]
BLAST of CmoCh15G003220 vs. NCBI nr
Match: gi|449449417|ref|XP_004142461.1| (PREDICTED: metalloendoproteinase 1-like [Cucumis sativus])

HSP 1 Score: 318.9 bits (816), Expect = 6.2e-84
Identity = 150/199 (75.38%), Postives = 165/199 (82.91%), Query Frame = 1 

Query: 1   MSKPRCGVADIIDGKSGMKSGKNMVNQHRK-SGHFHQVSHFTFFKGNLKWPNSKSHLTYG 60
           MS+PRCG ADI+DGK+ MKSGK MVNQHRK +GHFH VSH+ FF GN KWP SKSHLTYG
Sbjct: 115 MSRPRCGFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYG 174

Query: 61  FLPGTPSGAVDPVGRAFTTWAANTHFSFSLASSYKTADIKISFERGDHGDGFPFDNVGGV 120
           FLPGTPS AV  +GRAF TW ANTHF+FS  S YK ADIKISFE GDHGDG  FD V GV
Sbjct: 175 FLPGTPSKAVATIGRAFKTWGANTHFNFSQTSRYKKADIKISFETGDHGDGHSFDGVRGV 234

Query: 121 IAHAFAPTDGRLHFDAVERWTDGAVPNSYDVETVALHEIGHLLGLYHSSVEGAIMWPTIM 180
           IAH FAP DGRLHFDA E W  GA+ +S+D+ET+ALHEIGHLLGLYHSS+E AIMWP+IM
Sbjct: 235 IAHGFAPRDGRLHFDAAESWAVGAIADSFDLETIALHEIGHLLGLYHSSIERAIMWPSIM 294

Query: 181 RGATKGLHADDIAGIKALY 199
            G+TKGL ADDIAGI ALY
Sbjct: 295 EGSTKGLDADDIAGINALY 313

[Close]
BLAST of CmoCh15G003220 vs. NCBI nr
Match: gi|449449419|ref|XP_004142462.1| (PREDICTED: metalloendoproteinase 1 [Cucumis sativus])

HSP 1 Score: 293.5 bits (750), Expect = 2.8e-76
Identity = 138/199 (69.35%), Postives = 156/199 (78.39%), Query Frame = 1 

Query: 1   MSKPRCGVADIIDGKSGMKSGKNMVN-QHRKSGHFHQVSHFTFFKGNLKWPNSKSHLTYG 60
           MSKPRCGVADII G + M+S K     +H   GHFH VSHF FF GN KWP +KSHLTYG
Sbjct: 117 MSKPRCGVADIIHGNTWMRSSKKRKQLEHGIVGHFHSVSHFAFFDGNPKWPATKSHLTYG 176

Query: 61  FLPGTPSGAVDPVGRAFTTWAANTHFSFSLASSYKTADIKISFERGDHGDGFPFDNVGGV 120
           FLPGTPS  V PV RAF TWAAN+HF+FS A   +T+DI I FE GDHGDG+PFD VGGV
Sbjct: 177 FLPGTPSETVSPVARAFATWAANSHFTFSQALDNQTSDITIGFETGDHGDGYPFDGVGGV 236

Query: 121 IAHAFAPTDGRLHFDAVERWTDGAVPNSYDVETVALHEIGHLLGLYHSSVEGAIMWPTIM 180
           +AHAF+P+DGR H DA E W +G V +S+D+ETVALHEIGHLLGL HSS EGAIMWP + 
Sbjct: 237 VAHAFSPSDGRFHLDADESWANGVVSDSFDLETVALHEIGHLLGLQHSSTEGAIMWPQVP 296

Query: 181 RGATKGLHADDIAGIKALY 199
            GA+KGLH DDIAGIKALY
Sbjct: 297 VGASKGLHVDDIAGIKALY 315

The following BLAST results are available for this feature:
BLAST of CmoCh15G003220 vs. Swiss-Prot
Match NameE-valueIdentityDescription
2MMP_ARATH8.5e-4145.63Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana GN=2MMP PE=1 SV=1[more]
3MMP_ARATH4.2e-4043.27Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana GN=3MMP PE=1 SV=1[more]
MEP1_SOYBN2.6e-3744.12Metalloendoproteinase 1 OS=Glycine max PE=1 SV=2[more]
5MMP_ARATH4.8e-3644.76Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana GN=5MMP PE=1 SV=1[more]
1MMP_ARATH7.0e-3541.98Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana GN=1MMP PE=1 SV=1[more]
BLAST of CmoCh15G003220 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KS47_CUCSA1.0e-9684.00Uncharacterized protein OS=Cucumis sativus GN=Csa_5G633160 PE=3 SV=1[more]
A0A0A0KU08_CUCSA4.4e-8475.38Uncharacterized protein OS=Cucumis sativus GN=Csa_5G632130 PE=3 SV=1[more]
A0A0A0KS39_CUCSA2.0e-7669.35Uncharacterized protein OS=Cucumis sativus GN=Csa_5G632120 PE=3 SV=1[more]
Q9LEL9_CUCSA2.1e-7063.64Matrix metalloproteinase OS=Cucumis sativus GN=MMP-1 PE=2 SV=1[more]
B9RUG6_RICCO1.0e-6965.15Metalloendoproteinase 1, putative OS=Ricinus communis GN=RCOM_0852690 PE=3 SV=1[more]
BLAST of CmoCh15G003220 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G70170.14.8e-4245.63 matrix metalloproteinase[more]
AT1G24140.12.4e-4143.27 Matrixin family protein[more]
AT1G59970.12.7e-3744.76 Matrixin family protein[more]
AT4G16640.13.9e-3641.98 Matrixin family protein[more]
AT2G45040.14.5e-3238.65 Matrixin family protein[more]
BLAST of CmoCh15G003220 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|659117646|ref|XP_008458713.1|3.8e-9783.50PREDICTED: metalloendoproteinase 1-like [Cucumis melo][more]
gi|449449409|ref|XP_004142457.1|1.4e-9684.00PREDICTED: metalloendoproteinase 1-like [Cucumis sativus][more]
gi|659118019|ref|XP_008458907.1|3.5e-8779.40PREDICTED: metalloendoproteinase 1-like [Cucumis melo][more]
gi|449449417|ref|XP_004142461.1|6.2e-8475.38PREDICTED: metalloendoproteinase 1-like [Cucumis sativus][more]
gi|449449419|ref|XP_004142462.1|2.8e-7669.35PREDICTED: metalloendoproteinase 1 [Cucumis sativus][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001818Pept_M10_metallopeptidase
IPR006026Peptidase_Metallo
IPR021190Pept_M10A
IPR024079MetalloPept_cat_dom_sf
Vocabulary: Molecular Function
TermDefinition
GO:0004222metalloendopeptidase activity
GO:0008270zinc ion binding
GO:0008237metallopeptidase activity
Vocabulary: Biological Process
TermDefinition
GO:0006508proteolysis
Vocabulary: Cellular Component
TermDefinition
GO:0031012extracellular matrix
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0031012 extracellular matrix
molecular_function GO:0004222 metalloendopeptidase activity
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0005509 calcium ion binding
molecular_function GO:0008237 metallopeptidase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh15G003220.1CmoCh15G003220.1mRNA


InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata
Date Performed: 2017-05-19
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001818Peptidase M10, metallopeptidasePFAMPF00413Peptidase_M10coord: 49..199
score: 4.5
IPR006026Peptidase, metallopeptidaseSMARTSM00235col_5coord: 45..200
score: 1.6
IPR021190Peptidase M10APRINTSPR00138MATRIXINcoord: 72..87
score: 4.1E-36coord: 1..11
score: 4.1E-36coord: 186..199
score: 4.1E-36coord: 96..124
score: 4.1E-36coord: 153..178
score: 4.1
IPR024079Metallopeptidase, catalytic domainGENE3DG3DSA:3.40.390.10coord: 1..200
score: 1.3
NoneNo IPR availablePANTHERPTHR10201MATRIX METALLOPROTEINASEcoord: 35..198
score: 5.0E-75coord: 1..14
score: 5.0
NoneNo IPR availablePANTHERPTHR10201:SF140F20P5.11 PROTEINcoord: 1..14
score: 5.0E-75coord: 35..198
score: 5.0
NoneNo IPR availableunknownSSF55486Metalloproteases ("zincins"), catalytic domaincoord: 42..199
score: 4.26