CSPI05G28480 (gene) Wild cucumber (PI 183967)

Overview
NameCSPI05G28480
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionMatrix metalloproteinase
LocationChr5 : 26872519 .. 26873701 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAATAACTAATATCTCTTAAAGAGAAAATGGCCTCTCCCAAAGCTCTTCAAATTATCTTCCCTTTCACCCTTTTATTTCTTTCCCTCTTCCCTAATCCAAATACATCATCTCCAATTATCTTGAAACATAGTTCTCAAAACATGAACTCATCCAATTCCTTAATGTTTCTCAAGAATCTCCAAGGATGTCATTTAGGTGATACAAAACAAGGCATTCATCAAATAAAAAAATACCTTCAACGTTTTGGTTACATTACTACCAATATCCAAAAACACTCCAACCCCATCTTTGATGACACTTTTGATCATATCTTGGAATCTGCCCTTAAAACATACCAAACAAACCACAATCTTGCGCCCTCAGGAATCTTGGACTCCAACACCATTGCTCAAATAGCCATGCCTAGATGTGGAGTTCAAGATGTAATCAAAAACAAAAAAACCAAAAAAAGAAACCAAAATTTCACAAATAATGGCCATACTCATTTCCACAAAGTCTCTCACTTTACCTTCTTTGAAGGAAACCTGAAATGGCCATCTTCAAAGCTCCACTTAAGCTATGGATTTCTTCCAAACTACCCAATTGAAGCAATAAAACCAGTGTCAAGAGCTTTCTCAAAATGGAGTTTGAATACCCATTTCAAATTTTCACATGTTGCAGATTATAGAAAAGCTGATATCAAAATAAGCTTTGAAAGAGGCGAACATGGAGACAACGCTCCATTTGACGGTGTGGGAGGAGTTTTAGCTCATGCTTATGCACCAACGGATGGAAGGCTTCATTTTGATGGTGATGACGCTTGGTCTGTGGGTGCTATTTCAGGTTATTTTGATGTGGAAACTGTAGCTTTGCATGAGATTGGACATATTCTCGGGCTTCAACATAGCACTATTGAAGAGGCTATTATGTTTCCAAGTATACCAGAGGGAGTTACTAAGGGTTTGCATGGAGATGATATTGCTGGAATTAAGGCTCTTTATCGAGTTTGATACAATTGATACACTCTCAAAAGATATTTGTGTATACTTCTATTGGTTTTAGTTAGAATTTAAGTTAATTATTATGAAAAGTACATGTACAATGTCTTATTGTTGTTGAATAATGTTTAGGTTTGTTGTATTAGTTCTATCCAAATTAATATTGTGAATATATAATAGTGAAATTAAGTATATTTTATTTGTT

mRNA sequence

ATGGCCTCTCCCAAAGCTCTTCAAATTATCTTCCCTTTCACCCTTTTATTTCTTTCCCTCTTCCCTAATCCAAATACATCATCTCCAATTATCTTGAAACATAGTTCTCAAAACATGAACTCATCCAATTCCTTAATGTTTCTCAAGAATCTCCAAGGATGTCATTTAGGTGATACAAAACAAGGCATTCATCAAATAAAAAAATACCTTCAACGTTTTGGTTACATTACTACCAATATCCAAAAACACTCCAACCCCATCTTTGATGACACTTTTGATCATATCTTGGAATCTGCCCTTAAAACATACCAAACAAACCACAATCTTGCGCCCTCAGGAATCTTGGACTCCAACACCATTGCTCAAATAGCCATGCCTAGATGTGGAGTTCAAGATGTAATCAAAAACAAAAAAACCAAAAAAAGAAACCAAAATTTCACAAATAATGGCCATACTCATTTCCACAAAGTCTCTCACTTTACCTTCTTTGAAGGAAACCTGAAATGGCCATCTTCAAAGCTCCACTTAAGCTATGGATTTCTTCCAAACTACCCAATTGAAGCAATAAAACCAGTGTCAAGAGCTTTCTCAAAATGGAGTTTGAATACCCATTTCAAATTTTCACATGTTGCAGATTATAGAAAAGCTGATATCAAAATAAGCTTTGAAAGAGGCGAACATGGAGACAACGCTCCATTTGACGGTGTGGGAGGAGTTTTAGCTCATGCTTATGCACCAACGGATGGAAGGCTTCATTTTGATGGTGATGACGCTTGGTCTGTGGGTGCTATTTCAGGTTATTTTGATGTGGAAACTGTAGCTTTGCATGAGATTGGACATATTCTCGGGCTTCAACATAGCACTATTGAAGAGGCTATTATGTTTCCAAGTATACCAGAGGGAGTTACTAAGGGTTTGCATGGAGATGATATTGCTGGAATTAAGGCTCTTTATCGAGTTTGA

Coding sequence (CDS)

ATGGCCTCTCCCAAAGCTCTTCAAATTATCTTCCCTTTCACCCTTTTATTTCTTTCCCTCTTCCCTAATCCAAATACATCATCTCCAATTATCTTGAAACATAGTTCTCAAAACATGAACTCATCCAATTCCTTAATGTTTCTCAAGAATCTCCAAGGATGTCATTTAGGTGATACAAAACAAGGCATTCATCAAATAAAAAAATACCTTCAACGTTTTGGTTACATTACTACCAATATCCAAAAACACTCCAACCCCATCTTTGATGACACTTTTGATCATATCTTGGAATCTGCCCTTAAAACATACCAAACAAACCACAATCTTGCGCCCTCAGGAATCTTGGACTCCAACACCATTGCTCAAATAGCCATGCCTAGATGTGGAGTTCAAGATGTAATCAAAAACAAAAAAACCAAAAAAAGAAACCAAAATTTCACAAATAATGGCCATACTCATTTCCACAAAGTCTCTCACTTTACCTTCTTTGAAGGAAACCTGAAATGGCCATCTTCAAAGCTCCACTTAAGCTATGGATTTCTTCCAAACTACCCAATTGAAGCAATAAAACCAGTGTCAAGAGCTTTCTCAAAATGGAGTTTGAATACCCATTTCAAATTTTCACATGTTGCAGATTATAGAAAAGCTGATATCAAAATAAGCTTTGAAAGAGGCGAACATGGAGACAACGCTCCATTTGACGGTGTGGGAGGAGTTTTAGCTCATGCTTATGCACCAACGGATGGAAGGCTTCATTTTGATGGTGATGACGCTTGGTCTGTGGGTGCTATTTCAGGTTATTTTGATGTGGAAACTGTAGCTTTGCATGAGATTGGACATATTCTCGGGCTTCAACATAGCACTATTGAAGAGGCTATTATGTTTCCAAGTATACCAGAGGGAGTTACTAAGGGTTTGCATGGAGATGATATTGCTGGAATTAAGGCTCTTTATCGAGTTTGA
Homology
[Close]
BLAST of CSPI05G28480 vs. Swiss-Prot
Match: 2MMP_ARATH (Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana GN=2MMP PE=1 SV=1)

HSP 1 Score: 226.5 bits (576), Expect = 4.2e-58
Identity = 134/329 (40.73%), Postives = 187/329 (56.84%), Query Frame = 1 

Query: 10  IFPFTLLFLSL-------FPNPNTSSPIILKHSSQNMNSSNSLMFLKNLQGCHLGDTKQG 69
           +F F  LFL +       FPN +T+ P  L+++++    + S     N  GCH G    G
Sbjct: 5   VFGFLSLFLIVSPASAWFFPN-STAVPPSLRNTTRVFWDAFS-----NFTGCHHGQNVDG 64

Query: 70  IHQIKKYLQRFGYITTNIQKHSNPIFDDTFDHILESALKTYQTNHNLAPSGILDSNTIAQ 129
           +++IKKY QRFGYI      +    F D FD IL++A++ YQTN NL  +G LD+ TI  
Sbjct: 65  LYRIKKYFQRFGYIPETFSGN----FTDDFDDILKAAVELYQTNFNLNVTGELDALTIQH 124

Query: 130 IAMPRCGVQDVIKNKKTKKRNQNFT---NNGHTHFHKVSHFTFFEGNLKWPSSKLHLSYG 189
           I +PRCG  DV+         +  T   N   TH H V  +T F G  +WP ++  L+Y 
Sbjct: 125 IVIPRCGNPDVVNGTSLMHGGRRKTFEVNFSRTHLHAVKRYTLFPGEPRWPRNRRDLTYA 184

Query: 190 FLPNYPI-EAIKPV-SRAFSKWSLNTHFKFSHVADYRKADIKISFERGEHGDNAPFDGVG 249
           F P  P+ E +K V SRAF +WS  T   F+    +  +DI I F  G+HGD  PFDGV 
Sbjct: 185 FDPKNPLTEEVKSVFSRAFGRWSDVTALNFTLSESFSTSDITIGFYTGDHGDGEPFDGVL 244

Query: 250 GVLAHAYAPTDGRLHFDGDDAWSVG-------AISGYFDVETVALHEIGHILGLQHSTIE 309
           G LAHA++P  G+ H D D+ W V        +++   D+E+VA+HEIGH+LGL HS++E
Sbjct: 245 GTLAHAFSPPSGKFHLDADENWVVSGDLDSFLSVTAAVDLESVAVHEIGHLLGLGHSSVE 304

Query: 310 EAIMFPSIPEGVTK-GLHGDDIAGIKALY 319
           E+IM+P+I  G  K  L  DD+ GI+ LY
Sbjct: 305 ESIMYPTITTGKRKVDLTNDDVEGIQYLY 323

[Close]
BLAST of CSPI05G28480 vs. Swiss-Prot
Match: 5MMP_ARATH (Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana GN=5MMP PE=1 SV=1)

HSP 1 Score: 214.2 bits (544), Expect = 2.2e-54
Identity = 129/324 (39.81%), Postives = 178/324 (54.94%), Query Frame = 1 

Query: 9   IIFPFTLLFLS--LFPNPNTSSPIILKHSSQNMNSSNSLMFLKNLQGCHLGDTKQGIHQI 68
           +IF FT+  +S   + N ++  P+   +++QN   + S      L GCH+G+   G+ ++
Sbjct: 9   LIFFFTVNPISAKFYTNVSSIPPLQFLNATQNAWETFS-----KLAGCHIGENINGLSKL 68

Query: 69  KKYLQRFGYITTNIQKHSNPIFDDTFDHILESALKTYQTNHNLAPSGILDSNTIAQIAMP 128
           K+Y +RFGYITT           D FD +L+SA+ TYQ N NL  +G LDS+T+ QI  P
Sbjct: 69  KQYFRRFGYITTTGN------CTDDFDDVLQSAINTYQKNFNLKVTGKLDSSTLRQIVKP 128

Query: 129 RCGVQDVIKNKKTKKRNQNFTNNGHTHFHKVSHFTFFEGNLKWPSSKLHLSYGFLP--NY 188
           RCG  D+I              NG         ++FF G  +WP  K  L+Y F P  N 
Sbjct: 129 RCGNPDLIDGVSEM--------NGGKILRTTEKYSFFPGKPRWPKRKRDLTYAFAPQNNL 188

Query: 189 PIEAIKPVSRAFSKWSLNTHFKFSHVADYRKADIKISFERGEHGDNAPFDGVGGVLAHAY 248
             E  +  SRAF++W+  T   F+      +ADI I F  GEHGD  PFDG  G LAHA 
Sbjct: 189 TDEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEPFDGAMGTLAHAS 248

Query: 249 APTDGRLHFDGDDAWSVG---------AISGYFDVETVALHEIGHILGLQHSTIEEAIMF 308
           +P  G LH DGD+ W +           ++   D+E+VA+HEIGH+LGL HS++E+AIMF
Sbjct: 249 SPPTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGLGHSSVEDAIMF 308

Query: 309 PSIPEGVTK-GLHGDDIAGIKALY 319
           P+I  G  K  L  DDI GI+ LY
Sbjct: 309 PAISGGDRKVELAKDDIEGIQHLY 313

[Close]
BLAST of CSPI05G28480 vs. Swiss-Prot
Match: 3MMP_ARATH (Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana GN=3MMP PE=1 SV=1)

HSP 1 Score: 213.4 bits (542), Expect = 3.7e-54
Identity = 130/333 (39.04%), Postives = 186/333 (55.86%), Query Frame = 1 

Query: 10  IFPFTLLFLSLFP-------NPNTSSPIILKHSSQNMNSSNSLMFLKNLQGCHLGDTKQG 69
           +F   LLF +  P       N +   P +L++++ N  +S    FL N  GCH G    G
Sbjct: 6   VFMVFLLFFAPSPVSAGFYTNSSAIPPQLLRNATGNPWNS----FL-NFTGCHAGKKYDG 65

Query: 70  IHQIKKYLQRFGYIT-TNIQKHSNPIFDDTFDHILESALKTYQTNHNLAPSGILDSNTIA 129
           ++ +K+Y Q FGYIT TN+  +    F D FD IL++A++ YQ N  L  +G+LD  T+ 
Sbjct: 66  LYMLKQYFQHFGYITETNLSGN----FTDDFDDILKNAVEMYQRNFQLNVTGVLDELTLK 125

Query: 130 QIAMPRCGVQDVIKNKKT-----KKRNQNFTNNGHTHFHKVSHFTFFEGNLKWPSSKLHL 189
            + +PRCG  DV+    T     K    +F   G   FH V H++FF G  +WP ++  L
Sbjct: 126 HVVIPRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQ-RFHAVKHYSFFPGEPRWPRNRRDL 185

Query: 190 SYGFLP-NYPIEAIKPV-SRAFSKWSLNTHFKFSHVADYRKADIKISFERGEHGDNAPFD 249
           +Y F P N   E +K V SRAF++W   T   F+ V  +  +DI I F  GEHGD  PFD
Sbjct: 186 TYAFDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERFSTSDISIGFYSGEHGDGEPFD 245

Query: 250 GVGGVLAHAYAPTDGRLHFDGDDAWSVG--------AISGYFDVETVALHEIGHILGLQH 309
           G    LAHA++P  G  H DG++ W V         ++S   D+E+VA+HEIGH+LGL H
Sbjct: 246 GPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAVDLESVAVHEIGHLLGLGH 305

Query: 310 STIEEAIMFPSIPEGVTK-GLHGDDIAGIKALY 319
           S++E +IM+P+I  G  K  L  DD+ G++ LY
Sbjct: 306 SSVEGSIMYPTIRTGRRKVDLTTDDVEGVQYLY 328

[Close]
BLAST of CSPI05G28480 vs. Swiss-Prot
Match: MEP1_SOYBN (Metalloendoproteinase 1 OS=Glycine max PE=1 SV=2)

HSP 1 Score: 213.0 bits (541), Expect = 4.8e-54
Identity = 126/316 (39.87%), Postives = 166/316 (52.53%), Query Frame = 1 

Query: 14  TLLFLSLFPNPNTSSPIILKHSSQNMNSSNSLMFLKNLQGCHLGDTKQGIHQIKKYLQRF 73
           TL FL+      TS P +  H     +   +  F       H G   +G+  +K Y    
Sbjct: 15  TLYFLA------TSLPSVSAHGPYAWDGEATYKFTTY----HPGQNYKGLSNVKNYFHHL 74

Query: 74  GYITTNIQKHSNPIFDDTFDHILESALKTYQTNHNLAPSGILDSNTIAQIAMPRCGVQDV 133
           GYI       + P FDD FD  L SA+KTYQ N+NL  +G  D NT+ QI  PRCGV D+
Sbjct: 75  GYIP------NAPHFDDNFDDTLVSAIKTYQKNYNLNVTGKFDINTLKQIMTPRCGVPDI 134

Query: 134 IKNKKTKKRNQNFTNNGHTHFHKVSHFTFFEGNLKWPSSKLHLSYGFLPNYPIEAI--KP 193
           I N            N  T F  +S +TFF+   +W +    L+Y F P   ++      
Sbjct: 135 IIN-----------TNKTTSFGMISDYTFFKDMPRWQAGTTQLTYAFSPEPRLDDTFKSA 194

Query: 194 VSRAFSKWSLNTHFKFSHVADYRKADIKISFERGEHGDNAPFDGVGGVLAHAYAPTDGRL 253
           ++RAFSKW+   +  F     Y  A+IKI F    HGD  PFDG GG+L HA+APTDGR 
Sbjct: 195 IARAFSKWTPVVNIAFQETTSYETANIKILFASKNHGDPYPFDGPGGILGHAFAPTDGRC 254

Query: 254 HFDGDDAW------SVGAISGYFDVETVALHEIGHILGLQHSTIEEAIMFPSIPEGVTK- 313
           HFD D+ W      +   ++  FD+E+VA+HEIGH+LGL HS+   AIM+PSIP    K 
Sbjct: 255 HFDADEYWVASGDVTKSPVTSAFDLESVAVHEIGHLLGLGHSSDLRAIMYPSIPPRTRKV 303

Query: 314 GLHGDDIAGIKALYRV 321
            L  DDI GI+ LY +
Sbjct: 315 NLAQDDIDGIRKLYGI 303

[Close]
BLAST of CSPI05G28480 vs. Swiss-Prot
Match: 1MMP_ARATH (Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana GN=1MMP PE=1 SV=1)

HSP 1 Score: 191.4 bits (485), Expect = 1.5e-47
Identity = 116/297 (39.06%), Postives = 163/297 (54.88%), Query Frame = 1 

Query: 35  SSQNMNSSNSLMF-LKNLQGCHLGDTKQGIHQIKKYLQRFGYITTNIQKHSNPIFDDTFD 94
           ++Q ++ SNS       L    +G    G+ ++K+YL RFGY+    +     IF D FD
Sbjct: 41  ATQIIHVSNSTWHDFSRLVDVQIGSHVSGVSELKRYLHRFGYVNDGSE-----IFSDVFD 100

Query: 95  HILESALKTYQTNHNLAPSGILDSNTIAQIAMPRCGVQDVIKNKKTKKRNQNFTNNGHTH 154
             LESA+  YQ N  L  +G LD++T+  +++PRCGV D            + T N +  
Sbjct: 101 GPLESAISLYQENLGLPITGRLDTSTVTLMSLPRCGVSDT-----------HMTIN-NDF 160

Query: 155 FHKVSHFTFFEGNLKWPSSKLHLSYGFLPNYPI-----EAIKPV-SRAFSKWSLNTHFKF 214
            H  +H+T+F G  KW  ++  L+Y     + +     E +K V  RAFS+WS      F
Sbjct: 161 LHTTAHYTYFNGKPKW--NRDTLTYAISKTHKLDYLTSEDVKTVFRRAFSQWSSVIPVSF 220

Query: 215 SHVADYRKADIKISFERGEHGDNAPFDGVGGVLAHAYAPTDGRLHFDGDDAWSV-----G 274
             V D+  AD+KI F  G+HGD  PFDGV G LAHA+AP +GRLH D  + W V     G
Sbjct: 221 EEVDDFTTADLKIGFYAGDHGDGLPFDGVLGTLAHAFAPENGRLHLDAAETWIVDDDLKG 280

Query: 275 AISGYFDVETVALHEIGHILGLQHSTIEEAIMFPSI-PEGVTKGLHGDDIAGIKALY 319
           +     D+E+VA HEIGH+LGL HS+ E A+M+PS+ P      L  DD+AG+  LY
Sbjct: 281 SSEVAVDLESVATHEIGHLLGLGHSSQESAVMYPSLRPRTKKVDLTVDDVAGVLKLY 318

[Close]
BLAST of CSPI05G28480 vs. TrEMBL
Match: Q9LEL9_CUCSA (Matrix metalloproteinase OS=Cucumis sativus GN=MMP-1 PE=2 SV=1)

HSP 1 Score: 659.1 bits (1699), Expect = 2.8e-186
Identity = 319/320 (99.69%), Postives = 320/320 (100.00%), Query Frame = 1 

Query: 1   MASPKALQIIFPFTLLFLSLFPNPNTSSPIILKHSSQNMNSSNSLMFLKNLQGCHLGDTK 60
           MASPKALQIIFPFTLLFLSLFPNPNTSSPIILKHSSQNMNSSNSLMFLKNLQGCHLGDTK
Sbjct: 1   MASPKALQIIFPFTLLFLSLFPNPNTSSPIILKHSSQNMNSSNSLMFLKNLQGCHLGDTK 60

Query: 61  QGIHQIKKYLQRFGYITTNIQKHSNPIFDDTFDHILESALKTYQTNHNLAPSGILDSNTI 120
           QGIHQIKKYLQRFGYITTNIQKHSNPIFDDTFDHILESALKTYQTNHNLAPSGILDSNTI
Sbjct: 61  QGIHQIKKYLQRFGYITTNIQKHSNPIFDDTFDHILESALKTYQTNHNLAPSGILDSNTI 120

Query: 121 AQIAMPRCGVQDVIKNKKTKKRNQNFTNNGHTHFHKVSHFTFFEGNLKWPSSKLHLSYGF 180
           AQIAMPRCGVQDVIKNKKTKKRNQNFTNNGHTHFHKVSHFTFFEGNLKWPSSKLHLSYGF
Sbjct: 121 AQIAMPRCGVQDVIKNKKTKKRNQNFTNNGHTHFHKVSHFTFFEGNLKWPSSKLHLSYGF 180

Query: 181 LPNYPIEAIKPVSRAFSKWSLNTHFKFSHVADYRKADIKISFERGEHGDNAPFDGVGGVL 240
           LPNYPI+AIKPVSRAFSKWSLNTHFKFSHVADYRKADIKISFERGEHGDNAPFDGVGGVL
Sbjct: 181 LPNYPIDAIKPVSRAFSKWSLNTHFKFSHVADYRKADIKISFERGEHGDNAPFDGVGGVL 240

Query: 241 AHAYAPTDGRLHFDGDDAWSVGAISGYFDVETVALHEIGHILGLQHSTIEEAIMFPSIPE 300
           AHAYAPTDGRLHFDGDDAWSVGAISGYFDVETVALHEIGHILGLQHSTIEEAIMFPSIPE
Sbjct: 241 AHAYAPTDGRLHFDGDDAWSVGAISGYFDVETVALHEIGHILGLQHSTIEEAIMFPSIPE 300

Query: 301 GVTKGLHGDDIAGIKALYRV 321
           GVTKGLHGDDIAGIKALYRV
Sbjct: 301 GVTKGLHGDDIAGIKALYRV 320

[Close]
BLAST of CSPI05G28480 vs. TrEMBL
Match: A0A0H4TG97_MOMCH (Metalloendoproteinase 1-like OS=Momordica charantia PE=2 SV=1)

HSP 1 Score: 398.3 bits (1022), Expect = 9.0e-108
Identity = 202/321 (62.93%), Postives = 245/321 (76.32%), Query Frame = 1 

Query: 3   SPKALQIIFPFTLLFLSLFPNPNTSSPI-ILKHSS--QNMNSSNSLMFLKNLQGCHLGDT 62
           S +A QI      L LS FP+ N SS + ILKH++   +MNSS SL F  NL+GC LGDT
Sbjct: 2   SSQAFQIFAFILFLLLSFFPHLNMSSRVLILKHNAIAHDMNSSFSLSF-NNLRGCRLGDT 61

Query: 63  KQGIHQIKKYLQRFGYITTNIQKHSNPIFDDTFDHILESALKTYQTNHNLAPSGILDSNT 122
            QGIH++KKYL+RFGYIT N++K+S  + DDTFDH LESA+KTYQ N+N+  SGILD+ T
Sbjct: 62  AQGIHRLKKYLRRFGYIT-NVEKNSMAMDDDTFDHALESAVKTYQKNYNIDASGILDAKT 121

Query: 123 IAQIAMPRCGVQDVIKNKKTKKRNQNFTNNGHTHFHKVSHFTFFEGNLKWPSSKLHLSYG 182
           I Q+  PRCGVQD   N  T  R+     N    FH VSH++FF G  KWP+SK HLSY 
Sbjct: 122 ITQMRTPRCGVQDFF-NGTTWMRSSMDHKNRDGRFHTVSHYSFFGGKPKWPASKFHLSYA 181

Query: 183 FLPNYPIEAIKPVSRAFSKWSLNTHFKFSHVADYRKADIKISFERGEHGDNAPFDGVGGV 242
           FLP YP EAI+PV RAFSKW+  THF FS +++Y+K+DIKISFE  +HGD A FDG+GG+
Sbjct: 182 FLPGYPSEAIEPVDRAFSKWASETHFTFSKISNYKKSDIKISFEIEDHGDFASFDGMGGI 241

Query: 243 LAHAYAPTDGRLHFDGDDAWSVGAISGYFDVETVALHEIGHILGLQHSTIEEAIMFPSIP 302
           LAHA+ PTDGRLHFD  + WSVGA+SGYFDVETVALHEIGH+LGLQHS+IE AIM+P+IP
Sbjct: 242 LAHAFPPTDGRLHFDAHEPWSVGALSGYFDVETVALHEIGHLLGLQHSSIEAAIMYPTIP 301

Query: 303 EGVTKGLHGDDIAGIKALYRV 321
           EG TK LHGDD+AG+KALY +
Sbjct: 302 EGDTKDLHGDDVAGLKALYGI 319

[Close]
BLAST of CSPI05G28480 vs. TrEMBL
Match: A0A0A0KS47_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G633160 PE=3 SV=1)

HSP 1 Score: 361.7 bits (927), Expect = 9.3e-97
Identity = 180/318 (56.60%), Postives = 223/318 (70.13%), Query Frame = 1 

Query: 5   KALQIIFPFTL-LFLSLFPNPNTSSPIILKHSSQNMNSSNSLMFLKNLQGCHLGDTKQGI 64
           + L + F FTL L +SLFP  ++S P             + L+FLKNL+GC  GD  +GI
Sbjct: 4   QGLSLFFTFTLFLIISLFPLISSSQP----------KPVDGLVFLKNLKGCKKGDKVEGI 63

Query: 65  HQIKKYLQRFGYITTNIQKHSNPIFDDTFDHILESALKTYQTNHNLAPSGILDSNTIAQI 124
           HQ+KKYLQ FGY+  ++   S    DD FD ILE A+KTYQ N+NL  +G LD++T+ Q+
Sbjct: 64  HQMKKYLQHFGYLNNDVHIRSESS-DDEFDEILEFAVKTYQINYNLKVTGTLDNSTLMQM 123

Query: 125 AMPRCGVQDVIKNKKTKKRNQNFTNNGHT---HFHKVSHFTFFEGNLKWPSSKLHLSYGF 184
           + PRCGV D+I  K + K  + F N       HFH+VSHF FFEGNL+WP SK HL+YGF
Sbjct: 124 SKPRCGVADIINGKTSMKSGKRFVNQHRKISGHFHEVSHFAFFEGNLRWPDSKSHLTYGF 183

Query: 185 LPNYPIEAIKPVSRAFSKWSLNTHFKFSHVADYRKADIKISFERGEHGDNAPFDGVGGVL 244
           LP  P EAI PV+RAF+ W+ NTHF FS  + Y  ADIKISFE+G+HGD  PFD VGGVL
Sbjct: 184 LPGTPSEAISPVNRAFTTWAANTHFSFSQESKYENADIKISFEKGDHGDGFPFDSVGGVL 243

Query: 245 AHAYAPTDGRLHFDGDDAWSVGAISGYFDVETVALHEIGHILGLQHSTIEEAIMFPSIPE 304
           AHA+APTDGRLHFD  + W+ GA+   +D+ETVALHEIGH+LGL HS++E AIM+PSI  
Sbjct: 244 AHAFAPTDGRLHFDAVEHWADGAVPKSYDMETVALHEIGHLLGLHHSSVEGAIMWPSIMG 303

Query: 305 GVTKGLHGDDIAGIKALY 319
           G TKGLH DDI GIK LY
Sbjct: 304 GATKGLHADDIEGIKVLY 310

[Close]
BLAST of CSPI05G28480 vs. TrEMBL
Match: B9RUG6_RICCO (Metalloendoproteinase 1, putative OS=Ricinus communis GN=RCOM_0852690 PE=3 SV=1)

HSP 1 Score: 358.6 bits (919), Expect = 7.9e-96
Identity = 172/310 (55.48%), Postives = 219/310 (70.65%), Query Frame = 1 

Query: 12  PFTLLFLSLFPNPNTSSPIILKHSSQNMNSSNSLMFLKNLQGCHLGDTKQGIHQIKKYLQ 71
           PF +  ++L    +  S   L HS      S++  FLK+LQGCH GD  +GIH +KKYL+
Sbjct: 5   PFAIFSVTLILLISLLSSAALAHSHSKTEKSSAFDFLKHLQGCHKGDNLKGIHDLKKYLE 64

Query: 72  RFGYITTNIQKHSNPIFDDTFDHILESALKTYQTNHNLAPSGILDSNTIAQIAMPRCGVQ 131
            FGY++   Q HSN   DD FD +LE ALKTYQ N++L  +G LDS T+ ++ MPRCGV 
Sbjct: 65  NFGYLSYKNQSHSN---DDDFDDLLEYALKTYQFNYHLNVTGFLDSETVTKMMMPRCGVA 124

Query: 132 DVIKNK-KTKKRNQNFTNNGHTHFHKVSHFTFFEGNLKWPSSKLHLSYGFLPNYPIEAIK 191
           D+I    + +  N+N  ++  T FH VSH+ FF GN +WP+SK HL+YGFLP  P +A++
Sbjct: 125 DIINGTTRMQSSNKNPHHHSSTSFHTVSHYEFFPGNPRWPASKYHLTYGFLPGTPNQAME 184

Query: 192 PVSRAFSKWSLNTHFKFSHVADYRKADIKISFERGEHGDNAPFDGVGGVLAHAYAPTDGR 251
           PV++AF  W+ NTHF+F+ V DYR ADI I F RG+HGD +PFDG GG LAHA+AP DGR
Sbjct: 185 PVAKAFQTWAANTHFRFTRVQDYRAADITIGFHRGDHGDGSPFDGRGGTLAHAFAPQDGR 244

Query: 252 LHFDGDDAWSVGAISGYFDVETVALHEIGHILGLQHSTIEEAIMFPSIPEGVTKGLHGDD 311
            H+DGD+ W+VGA  G FDVETVALHEIGH+LGL HS++E AIM PSI  G TKGLH DD
Sbjct: 245 FHYDGDEHWAVGATQGAFDVETVALHEIGHLLGLGHSSVEGAIMHPSIQSGATKGLHSDD 304

Query: 312 IAGIKALYRV 321
           I GI+ALY V
Sbjct: 305 IQGIRALYNV 311

[Close]
BLAST of CSPI05G28480 vs. TrEMBL
Match: A0A0A0KU08_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G632130 PE=3 SV=1)

HSP 1 Score: 344.0 bits (881), Expect = 2.0e-91
Identity = 168/321 (52.34%), Postives = 214/321 (66.67%), Query Frame = 1 

Query: 1   MASPKALQIIFPFTLLFLSLFPNPNTSSPIILKHSSQNMNSSNSLMFLKNLQGCHLGDTK 60
           MA    L  +     L + LFP  ++S P          + SN L+FLKNL GC  GD  
Sbjct: 1   MAFETLLSFLTTSFFLVIVLFPLISSSHP--------KSHLSNGLVFLKNLNGCKKGDKV 60

Query: 61  QGIHQIKKYLQRFGYITTNIQKHSNPIFDDTFDHILESALKTYQTNHNLAPSGILDSNTI 120
           +GIHQ+K+YLQ FGY+   +Q +S    DD FD +LESA+KTYQ N+NL  +G +++ T+
Sbjct: 61  EGIHQVKEYLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTL 120

Query: 121 AQIAMPRCGVQDVIKNKKTKKRNQNFTNNGHT---HFHKVSHFTFFEGNLKWPSSKLHLS 180
             ++ PRCG  D++  K   K  +   N       HFH VSH+ FF+GN KWP+SK HL+
Sbjct: 121 DLMSRPRCGFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLT 180

Query: 181 YGFLPNYPIEAIKPVSRAFSKWSLNTHFKFSHVADYRKADIKISFERGEHGDNAPFDGVG 240
           YGFLP  P +A+  + RAF  W  NTHF FS  + Y+KADIKISFE G+HGD   FDGV 
Sbjct: 181 YGFLPGTPSKAVATIGRAFKTWGANTHFNFSQTSRYKKADIKISFETGDHGDGHSFDGVR 240

Query: 241 GVLAHAYAPTDGRLHFDGDDAWSVGAISGYFDVETVALHEIGHILGLQHSTIEEAIMFPS 300
           GV+AH +AP DGRLHFD  ++W+VGAI+  FD+ET+ALHEIGH+LGL HS+IE AIM+PS
Sbjct: 241 GVIAHGFAPRDGRLHFDAAESWAVGAIADSFDLETIALHEIGHLLGLYHSSIERAIMWPS 300

Query: 301 IPEGVTKGLHGDDIAGIKALY 319
           I EG TKGL  DDIAGI ALY
Sbjct: 301 IMEGSTKGLDADDIAGINALY 313

[Close]
BLAST of CSPI05G28480 vs. TAIR10
Match: AT1G70170.1 (AT1G70170.1 matrix metalloproteinase)

HSP 1 Score: 226.5 bits (576), Expect = 2.4e-59
Identity = 134/329 (40.73%), Postives = 187/329 (56.84%), Query Frame = 1 

Query: 10  IFPFTLLFLSL-------FPNPNTSSPIILKHSSQNMNSSNSLMFLKNLQGCHLGDTKQG 69
           +F F  LFL +       FPN +T+ P  L+++++    + S     N  GCH G    G
Sbjct: 5   VFGFLSLFLIVSPASAWFFPN-STAVPPSLRNTTRVFWDAFS-----NFTGCHHGQNVDG 64

Query: 70  IHQIKKYLQRFGYITTNIQKHSNPIFDDTFDHILESALKTYQTNHNLAPSGILDSNTIAQ 129
           +++IKKY QRFGYI      +    F D FD IL++A++ YQTN NL  +G LD+ TI  
Sbjct: 65  LYRIKKYFQRFGYIPETFSGN----FTDDFDDILKAAVELYQTNFNLNVTGELDALTIQH 124

Query: 130 IAMPRCGVQDVIKNKKTKKRNQNFT---NNGHTHFHKVSHFTFFEGNLKWPSSKLHLSYG 189
           I +PRCG  DV+         +  T   N   TH H V  +T F G  +WP ++  L+Y 
Sbjct: 125 IVIPRCGNPDVVNGTSLMHGGRRKTFEVNFSRTHLHAVKRYTLFPGEPRWPRNRRDLTYA 184

Query: 190 FLPNYPI-EAIKPV-SRAFSKWSLNTHFKFSHVADYRKADIKISFERGEHGDNAPFDGVG 249
           F P  P+ E +K V SRAF +WS  T   F+    +  +DI I F  G+HGD  PFDGV 
Sbjct: 185 FDPKNPLTEEVKSVFSRAFGRWSDVTALNFTLSESFSTSDITIGFYTGDHGDGEPFDGVL 244

Query: 250 GVLAHAYAPTDGRLHFDGDDAWSVG-------AISGYFDVETVALHEIGHILGLQHSTIE 309
           G LAHA++P  G+ H D D+ W V        +++   D+E+VA+HEIGH+LGL HS++E
Sbjct: 245 GTLAHAFSPPSGKFHLDADENWVVSGDLDSFLSVTAAVDLESVAVHEIGHLLGLGHSSVE 304

Query: 310 EAIMFPSIPEGVTK-GLHGDDIAGIKALY 319
           E+IM+P+I  G  K  L  DD+ GI+ LY
Sbjct: 305 ESIMYPTITTGKRKVDLTNDDVEGIQYLY 323

[Close]
BLAST of CSPI05G28480 vs. TAIR10
Match: AT1G59970.1 (AT1G59970.1 Matrixin family protein)

HSP 1 Score: 214.2 bits (544), Expect = 1.2e-55
Identity = 129/324 (39.81%), Postives = 178/324 (54.94%), Query Frame = 1 

Query: 9   IIFPFTLLFLS--LFPNPNTSSPIILKHSSQNMNSSNSLMFLKNLQGCHLGDTKQGIHQI 68
           +IF FT+  +S   + N ++  P+   +++QN   + S      L GCH+G+   G+ ++
Sbjct: 9   LIFFFTVNPISAKFYTNVSSIPPLQFLNATQNAWETFS-----KLAGCHIGENINGLSKL 68

Query: 69  KKYLQRFGYITTNIQKHSNPIFDDTFDHILESALKTYQTNHNLAPSGILDSNTIAQIAMP 128
           K+Y +RFGYITT           D FD +L+SA+ TYQ N NL  +G LDS+T+ QI  P
Sbjct: 69  KQYFRRFGYITTTGN------CTDDFDDVLQSAINTYQKNFNLKVTGKLDSSTLRQIVKP 128

Query: 129 RCGVQDVIKNKKTKKRNQNFTNNGHTHFHKVSHFTFFEGNLKWPSSKLHLSYGFLP--NY 188
           RCG  D+I              NG         ++FF G  +WP  K  L+Y F P  N 
Sbjct: 129 RCGNPDLIDGVSEM--------NGGKILRTTEKYSFFPGKPRWPKRKRDLTYAFAPQNNL 188

Query: 189 PIEAIKPVSRAFSKWSLNTHFKFSHVADYRKADIKISFERGEHGDNAPFDGVGGVLAHAY 248
             E  +  SRAF++W+  T   F+      +ADI I F  GEHGD  PFDG  G LAHA 
Sbjct: 189 TDEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEPFDGAMGTLAHAS 248

Query: 249 APTDGRLHFDGDDAWSVG---------AISGYFDVETVALHEIGHILGLQHSTIEEAIMF 308
           +P  G LH DGD+ W +           ++   D+E+VA+HEIGH+LGL HS++E+AIMF
Sbjct: 249 SPPTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGLGHSSVEDAIMF 308

Query: 309 PSIPEGVTK-GLHGDDIAGIKALY 319
           P+I  G  K  L  DDI GI+ LY
Sbjct: 309 PAISGGDRKVELAKDDIEGIQHLY 313

[Close]
BLAST of CSPI05G28480 vs. TAIR10
Match: AT1G24140.1 (AT1G24140.1 Matrixin family protein)

HSP 1 Score: 213.4 bits (542), Expect = 2.1e-55
Identity = 130/333 (39.04%), Postives = 186/333 (55.86%), Query Frame = 1 

Query: 10  IFPFTLLFLSLFP-------NPNTSSPIILKHSSQNMNSSNSLMFLKNLQGCHLGDTKQG 69
           +F   LLF +  P       N +   P +L++++ N  +S    FL N  GCH G    G
Sbjct: 6   VFMVFLLFFAPSPVSAGFYTNSSAIPPQLLRNATGNPWNS----FL-NFTGCHAGKKYDG 65

Query: 70  IHQIKKYLQRFGYIT-TNIQKHSNPIFDDTFDHILESALKTYQTNHNLAPSGILDSNTIA 129
           ++ +K+Y Q FGYIT TN+  +    F D FD IL++A++ YQ N  L  +G+LD  T+ 
Sbjct: 66  LYMLKQYFQHFGYITETNLSGN----FTDDFDDILKNAVEMYQRNFQLNVTGVLDELTLK 125

Query: 130 QIAMPRCGVQDVIKNKKT-----KKRNQNFTNNGHTHFHKVSHFTFFEGNLKWPSSKLHL 189
            + +PRCG  DV+    T     K    +F   G   FH V H++FF G  +WP ++  L
Sbjct: 126 HVVIPRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQ-RFHAVKHYSFFPGEPRWPRNRRDL 185

Query: 190 SYGFLP-NYPIEAIKPV-SRAFSKWSLNTHFKFSHVADYRKADIKISFERGEHGDNAPFD 249
           +Y F P N   E +K V SRAF++W   T   F+ V  +  +DI I F  GEHGD  PFD
Sbjct: 186 TYAFDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERFSTSDISIGFYSGEHGDGEPFD 245

Query: 250 GVGGVLAHAYAPTDGRLHFDGDDAWSVG--------AISGYFDVETVALHEIGHILGLQH 309
           G    LAHA++P  G  H DG++ W V         ++S   D+E+VA+HEIGH+LGL H
Sbjct: 246 GPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAVDLESVAVHEIGHLLGLGH 305

Query: 310 STIEEAIMFPSIPEGVTK-GLHGDDIAGIKALY 319
           S++E +IM+P+I  G  K  L  DD+ G++ LY
Sbjct: 306 SSVEGSIMYPTIRTGRRKVDLTTDDVEGVQYLY 328

[Close]
BLAST of CSPI05G28480 vs. TAIR10
Match: AT4G16640.1 (AT4G16640.1 Matrixin family protein)

HSP 1 Score: 191.4 bits (485), Expect = 8.4e-49
Identity = 116/297 (39.06%), Postives = 163/297 (54.88%), Query Frame = 1 

Query: 35  SSQNMNSSNSLMF-LKNLQGCHLGDTKQGIHQIKKYLQRFGYITTNIQKHSNPIFDDTFD 94
           ++Q ++ SNS       L    +G    G+ ++K+YL RFGY+    +     IF D FD
Sbjct: 41  ATQIIHVSNSTWHDFSRLVDVQIGSHVSGVSELKRYLHRFGYVNDGSE-----IFSDVFD 100

Query: 95  HILESALKTYQTNHNLAPSGILDSNTIAQIAMPRCGVQDVIKNKKTKKRNQNFTNNGHTH 154
             LESA+  YQ N  L  +G LD++T+  +++PRCGV D            + T N +  
Sbjct: 101 GPLESAISLYQENLGLPITGRLDTSTVTLMSLPRCGVSDT-----------HMTIN-NDF 160

Query: 155 FHKVSHFTFFEGNLKWPSSKLHLSYGFLPNYPI-----EAIKPV-SRAFSKWSLNTHFKF 214
            H  +H+T+F G  KW  ++  L+Y     + +     E +K V  RAFS+WS      F
Sbjct: 161 LHTTAHYTYFNGKPKW--NRDTLTYAISKTHKLDYLTSEDVKTVFRRAFSQWSSVIPVSF 220

Query: 215 SHVADYRKADIKISFERGEHGDNAPFDGVGGVLAHAYAPTDGRLHFDGDDAWSV-----G 274
             V D+  AD+KI F  G+HGD  PFDGV G LAHA+AP +GRLH D  + W V     G
Sbjct: 221 EEVDDFTTADLKIGFYAGDHGDGLPFDGVLGTLAHAFAPENGRLHLDAAETWIVDDDLKG 280

Query: 275 AISGYFDVETVALHEIGHILGLQHSTIEEAIMFPSI-PEGVTKGLHGDDIAGIKALY 319
           +     D+E+VA HEIGH+LGL HS+ E A+M+PS+ P      L  DD+AG+  LY
Sbjct: 281 SSEVAVDLESVATHEIGHLLGLGHSSQESAVMYPSLRPRTKKVDLTVDDVAGVLKLY 318

[Close]
BLAST of CSPI05G28480 vs. TAIR10
Match: AT2G45040.1 (AT2G45040.1 Matrixin family protein)

HSP 1 Score: 172.6 bits (436), Expect = 4.1e-43
Identity = 116/326 (35.58%), Postives = 164/326 (50.31%), Query Frame = 1 

Query: 5   KALQIIFPFTLLFLSLFPNPNTSSPIILKHSSQNMNSSNSLMFLKNLQGCHLGDTKQGIH 64
           K    IF F LL+L+L             H+ Q + + N   F  N            I 
Sbjct: 11  KPFTTIFSFFLLYLNL-------------HNQQIIEARNPSQFTTN------PSPDVSIP 70

Query: 65  QIKKYLQRFGYITTNIQKHSNPIFDDTFDHILESALKTYQTNHNLAPSGILDSNTIAQIA 124
           +IK++LQ++GY+  N  K S+       D   E AL  YQ N  L  +G  DS+T++QI 
Sbjct: 71  EIKRHLQQYGYLPQN--KESD-------DVSFEQALVRYQKNLGLPITGKPDSDTLSQIL 130

Query: 125 MPRCGVQDVIKNKKTKKRNQNFTNNGHTHFHKVSHFTFFEGNLKWPSS-KLHLSYGF--- 184
           +PRCG  D ++ K                FH    + +F G  +W     L L+Y F   
Sbjct: 131 LPRCGFPDDVEPKTAP-------------FHTGKKYVYFPGRPRWTRDVPLKLTYAFSQE 190

Query: 185 -LPNY--PIEAIKPVSRAFSKWSLNTHFKFSHVADYRKADIKISFERGEHGDNAPFDGVG 244
            L  Y  P +  +   RAF KW+      F    DY  ADIKI F  G+HGD  PFDGV 
Sbjct: 191 NLTPYLAPTDIRRVFRRAFGKWASVIPVSFIETEDYVIADIKIGFFNGDHGDGEPFDGVL 250

Query: 245 GVLAHAYAPTDGRLHFDGDDAWSVG----AISGYFDVETVALHEIGHILGLQHSTIEEAI 304
           GVLAH ++P +GRLH D  + W+V       S   D+E+VA+HEIGH+LGL HS++++A 
Sbjct: 251 GVLAHTFSPENGRLHLDKAETWAVDFDEEKSSVAVDLESVAVHEIGHVLGLGHSSVKDAA 295

Query: 305 MFPSI-PEGVTKGLHGDDIAGIKALY 319
           M+P++ P      L+ DD+ G+++LY
Sbjct: 311 MYPTLKPRSKKVNLNMDDVVGVQSLY 295

[Close]
BLAST of CSPI05G28480 vs. NCBI nr
Match: gi|525507280|ref|NP_001267680.1| (metalloendoproteinase 1-like precursor [Cucumis sativus])

HSP 1 Score: 659.1 bits (1699), Expect = 4.0e-186
Identity = 319/320 (99.69%), Postives = 320/320 (100.00%), Query Frame = 1 

Query: 1   MASPKALQIIFPFTLLFLSLFPNPNTSSPIILKHSSQNMNSSNSLMFLKNLQGCHLGDTK 60
           MASPKALQIIFPFTLLFLSLFPNPNTSSPIILKHSSQNMNSSNSLMFLKNLQGCHLGDTK
Sbjct: 1   MASPKALQIIFPFTLLFLSLFPNPNTSSPIILKHSSQNMNSSNSLMFLKNLQGCHLGDTK 60

Query: 61  QGIHQIKKYLQRFGYITTNIQKHSNPIFDDTFDHILESALKTYQTNHNLAPSGILDSNTI 120
           QGIHQIKKYLQRFGYITTNIQKHSNPIFDDTFDHILESALKTYQTNHNLAPSGILDSNTI
Sbjct: 61  QGIHQIKKYLQRFGYITTNIQKHSNPIFDDTFDHILESALKTYQTNHNLAPSGILDSNTI 120

Query: 121 AQIAMPRCGVQDVIKNKKTKKRNQNFTNNGHTHFHKVSHFTFFEGNLKWPSSKLHLSYGF 180
           AQIAMPRCGVQDVIKNKKTKKRNQNFTNNGHTHFHKVSHFTFFEGNLKWPSSKLHLSYGF
Sbjct: 121 AQIAMPRCGVQDVIKNKKTKKRNQNFTNNGHTHFHKVSHFTFFEGNLKWPSSKLHLSYGF 180

Query: 181 LPNYPIEAIKPVSRAFSKWSLNTHFKFSHVADYRKADIKISFERGEHGDNAPFDGVGGVL 240
           LPNYPI+AIKPVSRAFSKWSLNTHFKFSHVADYRKADIKISFERGEHGDNAPFDGVGGVL
Sbjct: 181 LPNYPIDAIKPVSRAFSKWSLNTHFKFSHVADYRKADIKISFERGEHGDNAPFDGVGGVL 240

Query: 241 AHAYAPTDGRLHFDGDDAWSVGAISGYFDVETVALHEIGHILGLQHSTIEEAIMFPSIPE 300
           AHAYAPTDGRLHFDGDDAWSVGAISGYFDVETVALHEIGHILGLQHSTIEEAIMFPSIPE
Sbjct: 241 AHAYAPTDGRLHFDGDDAWSVGAISGYFDVETVALHEIGHILGLQHSTIEEAIMFPSIPE 300

Query: 301 GVTKGLHGDDIAGIKALYRV 321
           GVTKGLHGDDIAGIKALYRV
Sbjct: 301 GVTKGLHGDDIAGIKALYRV 320

[Close]
BLAST of CSPI05G28480 vs. NCBI nr
Match: gi|659117650|ref|XP_008458715.1| (PREDICTED: metalloendoproteinase 1-like [Cucumis melo])

HSP 1 Score: 614.0 bits (1582), Expect = 1.5e-172
Identity = 298/320 (93.12%), Postives = 305/320 (95.31%), Query Frame = 1 

Query: 1   MASPKALQIIFPFTLLFLSLFPNPNTSSPIILKHSSQNMNSSNSLMFLKNLQGCHLGDTK 60
           MAS KA+QIIFPFTLLFLSLFPN NTSSP+ILKH+S NMNSSNSLMFLKNLQGCHLGDTK
Sbjct: 1   MASSKAIQIIFPFTLLFLSLFPNLNTSSPMILKHNSHNMNSSNSLMFLKNLQGCHLGDTK 60

Query: 61  QGIHQIKKYLQRFGYITTNIQKHSNPIFDDTFDHILESALKTYQTNHNLAPSGILDSNTI 120
           QGI QIKKYLQRFGYITTNIQKHSNPIFDDTFD +LESALKTYQTNHNLAPSGILDSNT+
Sbjct: 61  QGIQQIKKYLQRFGYITTNIQKHSNPIFDDTFDQVLESALKTYQTNHNLAPSGILDSNTL 120

Query: 121 AQIAMPRCGVQDVIKNKKTKKRNQNFTNNGHTHFHKVSHFTFFEGNLKWPSSKLHLSYGF 180
           AQIA PRCGVQDVIKNKK   RNQN+ NNGH HFHKVSHFTFFEGNLKWPSSK HLSYGF
Sbjct: 121 AQIAKPRCGVQDVIKNKK---RNQNYKNNGHIHFHKVSHFTFFEGNLKWPSSKFHLSYGF 180

Query: 181 LPNYPIEAIKPVSRAFSKWSLNTHFKFSHVADYRKADIKISFERGEHGDNAPFDGVGGVL 240
           LPNYPIEAIKPVSRAFSKWSLNTHFKFSHV DYRKADIKISFERGEHGDNAPFDGVGGVL
Sbjct: 181 LPNYPIEAIKPVSRAFSKWSLNTHFKFSHVVDYRKADIKISFERGEHGDNAPFDGVGGVL 240

Query: 241 AHAYAPTDGRLHFDGDDAWSVGAISGYFDVETVALHEIGHILGLQHSTIEEAIMFPSIPE 300
           AHAY PTDGRLHFDGD+AWSVGAISGYFDVETVALHEIGHILGLQHSTIEEAIMFPSIPE
Sbjct: 241 AHAYPPTDGRLHFDGDEAWSVGAISGYFDVETVALHEIGHILGLQHSTIEEAIMFPSIPE 300

Query: 301 GVTKGLHGDDIAGIKALYRV 321
           GVTKGLHGDDIAGIK LYRV
Sbjct: 301 GVTKGLHGDDIAGIKTLYRV 317

[Close]
BLAST of CSPI05G28480 vs. NCBI nr
Match: gi|874969612|gb|AKQ06192.1| (metalloendoproteinase 1-like precursor [Momordica charantia])

HSP 1 Score: 398.3 bits (1022), Expect = 1.3e-107
Identity = 202/321 (62.93%), Postives = 245/321 (76.32%), Query Frame = 1 

Query: 3   SPKALQIIFPFTLLFLSLFPNPNTSSPI-ILKHSS--QNMNSSNSLMFLKNLQGCHLGDT 62
           S +A QI      L LS FP+ N SS + ILKH++   +MNSS SL F  NL+GC LGDT
Sbjct: 2   SSQAFQIFAFILFLLLSFFPHLNMSSRVLILKHNAIAHDMNSSFSLSF-NNLRGCRLGDT 61

Query: 63  KQGIHQIKKYLQRFGYITTNIQKHSNPIFDDTFDHILESALKTYQTNHNLAPSGILDSNT 122
            QGIH++KKYL+RFGYIT N++K+S  + DDTFDH LESA+KTYQ N+N+  SGILD+ T
Sbjct: 62  AQGIHRLKKYLRRFGYIT-NVEKNSMAMDDDTFDHALESAVKTYQKNYNIDASGILDAKT 121

Query: 123 IAQIAMPRCGVQDVIKNKKTKKRNQNFTNNGHTHFHKVSHFTFFEGNLKWPSSKLHLSYG 182
           I Q+  PRCGVQD   N  T  R+     N    FH VSH++FF G  KWP+SK HLSY 
Sbjct: 122 ITQMRTPRCGVQDFF-NGTTWMRSSMDHKNRDGRFHTVSHYSFFGGKPKWPASKFHLSYA 181

Query: 183 FLPNYPIEAIKPVSRAFSKWSLNTHFKFSHVADYRKADIKISFERGEHGDNAPFDGVGGV 242
           FLP YP EAI+PV RAFSKW+  THF FS +++Y+K+DIKISFE  +HGD A FDG+GG+
Sbjct: 182 FLPGYPSEAIEPVDRAFSKWASETHFTFSKISNYKKSDIKISFEIEDHGDFASFDGMGGI 241

Query: 243 LAHAYAPTDGRLHFDGDDAWSVGAISGYFDVETVALHEIGHILGLQHSTIEEAIMFPSIP 302
           LAHA+ PTDGRLHFD  + WSVGA+SGYFDVETVALHEIGH+LGLQHS+IE AIM+P+IP
Sbjct: 242 LAHAFPPTDGRLHFDAHEPWSVGALSGYFDVETVALHEIGHLLGLQHSSIEAAIMYPTIP 301

Query: 303 EGVTKGLHGDDIAGIKALYRV 321
           EG TK LHGDD+AG+KALY +
Sbjct: 302 EGDTKDLHGDDVAGLKALYGI 319

[Close]
BLAST of CSPI05G28480 vs. NCBI nr
Match: gi|449449409|ref|XP_004142457.1| (PREDICTED: metalloendoproteinase 1-like [Cucumis sativus])

HSP 1 Score: 361.7 bits (927), Expect = 1.3e-96
Identity = 180/318 (56.60%), Postives = 223/318 (70.13%), Query Frame = 1 

Query: 5   KALQIIFPFTL-LFLSLFPNPNTSSPIILKHSSQNMNSSNSLMFLKNLQGCHLGDTKQGI 64
           + L + F FTL L +SLFP  ++S P             + L+FLKNL+GC  GD  +GI
Sbjct: 4   QGLSLFFTFTLFLIISLFPLISSSQP----------KPVDGLVFLKNLKGCKKGDKVEGI 63

Query: 65  HQIKKYLQRFGYITTNIQKHSNPIFDDTFDHILESALKTYQTNHNLAPSGILDSNTIAQI 124
           HQ+KKYLQ FGY+  ++   S    DD FD ILE A+KTYQ N+NL  +G LD++T+ Q+
Sbjct: 64  HQMKKYLQHFGYLNNDVHIRSESS-DDEFDEILEFAVKTYQINYNLKVTGTLDNSTLMQM 123

Query: 125 AMPRCGVQDVIKNKKTKKRNQNFTNNGHT---HFHKVSHFTFFEGNLKWPSSKLHLSYGF 184
           + PRCGV D+I  K + K  + F N       HFH+VSHF FFEGNL+WP SK HL+YGF
Sbjct: 124 SKPRCGVADIINGKTSMKSGKRFVNQHRKISGHFHEVSHFAFFEGNLRWPDSKSHLTYGF 183

Query: 185 LPNYPIEAIKPVSRAFSKWSLNTHFKFSHVADYRKADIKISFERGEHGDNAPFDGVGGVL 244
           LP  P EAI PV+RAF+ W+ NTHF FS  + Y  ADIKISFE+G+HGD  PFD VGGVL
Sbjct: 184 LPGTPSEAISPVNRAFTTWAANTHFSFSQESKYENADIKISFEKGDHGDGFPFDSVGGVL 243

Query: 245 AHAYAPTDGRLHFDGDDAWSVGAISGYFDVETVALHEIGHILGLQHSTIEEAIMFPSIPE 304
           AHA+APTDGRLHFD  + W+ GA+   +D+ETVALHEIGH+LGL HS++E AIM+PSI  
Sbjct: 244 AHAFAPTDGRLHFDAVEHWADGAVPKSYDMETVALHEIGHLLGLHHSSVEGAIMWPSIMG 303

Query: 305 GVTKGLHGDDIAGIKALY 319
           G TKGLH DDI GIK LY
Sbjct: 304 GATKGLHADDIEGIKVLY 310

[Close]
BLAST of CSPI05G28480 vs. NCBI nr
Match: gi|255552736|ref|XP_002517411.1| (PREDICTED: metalloendoproteinase 3-MMP [Ricinus communis])

HSP 1 Score: 358.6 bits (919), Expect = 1.1e-95
Identity = 172/310 (55.48%), Postives = 219/310 (70.65%), Query Frame = 1 

Query: 12  PFTLLFLSLFPNPNTSSPIILKHSSQNMNSSNSLMFLKNLQGCHLGDTKQGIHQIKKYLQ 71
           PF +  ++L    +  S   L HS      S++  FLK+LQGCH GD  +GIH +KKYL+
Sbjct: 5   PFAIFSVTLILLISLLSSAALAHSHSKTEKSSAFDFLKHLQGCHKGDNLKGIHDLKKYLE 64

Query: 72  RFGYITTNIQKHSNPIFDDTFDHILESALKTYQTNHNLAPSGILDSNTIAQIAMPRCGVQ 131
            FGY++   Q HSN   DD FD +LE ALKTYQ N++L  +G LDS T+ ++ MPRCGV 
Sbjct: 65  NFGYLSYKNQSHSN---DDDFDDLLEYALKTYQFNYHLNVTGFLDSETVTKMMMPRCGVA 124

Query: 132 DVIKNK-KTKKRNQNFTNNGHTHFHKVSHFTFFEGNLKWPSSKLHLSYGFLPNYPIEAIK 191
           D+I    + +  N+N  ++  T FH VSH+ FF GN +WP+SK HL+YGFLP  P +A++
Sbjct: 125 DIINGTTRMQSSNKNPHHHSSTSFHTVSHYEFFPGNPRWPASKYHLTYGFLPGTPNQAME 184

Query: 192 PVSRAFSKWSLNTHFKFSHVADYRKADIKISFERGEHGDNAPFDGVGGVLAHAYAPTDGR 251
           PV++AF  W+ NTHF+F+ V DYR ADI I F RG+HGD +PFDG GG LAHA+AP DGR
Sbjct: 185 PVAKAFQTWAANTHFRFTRVQDYRAADITIGFHRGDHGDGSPFDGRGGTLAHAFAPQDGR 244

Query: 252 LHFDGDDAWSVGAISGYFDVETVALHEIGHILGLQHSTIEEAIMFPSIPEGVTKGLHGDD 311
            H+DGD+ W+VGA  G FDVETVALHEIGH+LGL HS++E AIM PSI  G TKGLH DD
Sbjct: 245 FHYDGDEHWAVGATQGAFDVETVALHEIGHLLGLGHSSVEGAIMHPSIQSGATKGLHSDD 304

Query: 312 IAGIKALYRV 321
           I GI+ALY V
Sbjct: 305 IQGIRALYNV 311

The following BLAST results are available for this feature:
BLAST of CSPI05G28480 vs. Swiss-Prot
Match NameE-valueIdentityDescription
2MMP_ARATH4.2e-5840.73Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana GN=2MMP PE=1 SV=1[more]
5MMP_ARATH2.2e-5439.81Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana GN=5MMP PE=1 SV=1[more]
3MMP_ARATH3.7e-5439.04Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana GN=3MMP PE=1 SV=1[more]
MEP1_SOYBN4.8e-5439.87Metalloendoproteinase 1 OS=Glycine max PE=1 SV=2[more]
1MMP_ARATH1.5e-4739.06Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana GN=1MMP PE=1 SV=1[more]
BLAST of CSPI05G28480 vs. TrEMBL
Match NameE-valueIdentityDescription
Q9LEL9_CUCSA2.8e-18699.69Matrix metalloproteinase OS=Cucumis sativus GN=MMP-1 PE=2 SV=1[more]
A0A0H4TG97_MOMCH9.0e-10862.93Metalloendoproteinase 1-like OS=Momordica charantia PE=2 SV=1[more]
A0A0A0KS47_CUCSA9.3e-9756.60Uncharacterized protein OS=Cucumis sativus GN=Csa_5G633160 PE=3 SV=1[more]
B9RUG6_RICCO7.9e-9655.48Metalloendoproteinase 1, putative OS=Ricinus communis GN=RCOM_0852690 PE=3 SV=1[more]
A0A0A0KU08_CUCSA2.0e-9152.34Uncharacterized protein OS=Cucumis sativus GN=Csa_5G632130 PE=3 SV=1[more]
BLAST of CSPI05G28480 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G70170.12.4e-5940.73 matrix metalloproteinase[more]
AT1G59970.11.2e-5539.81 Matrixin family protein[more]
AT1G24140.12.1e-5539.04 Matrixin family protein[more]
AT4G16640.18.4e-4939.06 Matrixin family protein[more]
AT2G45040.14.1e-4335.58 Matrixin family protein[more]
BLAST of CSPI05G28480 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|525507280|ref|NP_001267680.1|4.0e-18699.69metalloendoproteinase 1-like precursor [Cucumis sativus][more]
gi|659117650|ref|XP_008458715.1|1.5e-17293.13PREDICTED: metalloendoproteinase 1-like [Cucumis melo][more]
gi|874969612|gb|AKQ06192.1|1.3e-10762.93metalloendoproteinase 1-like precursor [Momordica charantia][more]
gi|449449409|ref|XP_004142457.1|1.3e-9656.60PREDICTED: metalloendoproteinase 1-like [Cucumis sativus][more]
gi|255552736|ref|XP_002517411.1|1.1e-9555.48PREDICTED: metalloendoproteinase 3-MMP [Ricinus communis][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001818Pept_M10_metallopeptidase
IPR002477Peptidoglycan-bd-like
IPR006026Peptidase_Metallo
IPR021190Pept_M10A
IPR024079MetalloPept_cat_dom_sf
Vocabulary: Molecular Function
TermDefinition
GO:0004222metalloendopeptidase activity
GO:0008270zinc ion binding
GO:0008237metallopeptidase activity
Vocabulary: Biological Process
TermDefinition
GO:0006508proteolysis
Vocabulary: Cellular Component
TermDefinition
GO:0031012extracellular matrix
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0031012 extracellular matrix
molecular_function GO:0004222 metalloendopeptidase activity
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0008237 metallopeptidase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI05G28480.1CSPI05G28480.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001818Peptidase M10, metallopeptidasePFAMPF00413Peptidase_M10coord: 188..318
score: 2.0
IPR002477Peptidoglycan binding-likeGENE3DG3DSA:1.10.101.10coord: 62..124
score: 1.6
IPR002477Peptidoglycan binding-likePFAMPF01471PG_binding_1coord: 61..122
score: 7.2
IPR002477Peptidoglycan binding-likeunknownSSF47090PGBD-likecoord: 54..133
score: 1.37
IPR006026Peptidase, metallopeptidaseSMARTSM00235col_5coord: 165..320
score: 6.6
IPR021190Peptidase M10APRINTSPR00138MATRIXINcoord: 192..207
score: 4.2E-34coord: 273..298
score: 4.2E-34coord: 123..136
score: 4.2E-34coord: 216..244
score: 4.2E-34coord: 306..319
score: 4.2
IPR024079Metallopeptidase, catalytic domainGENE3DG3DSA:3.40.390.10coord: 125..319
score: 8.9
NoneNo IPR availablePANTHERPTHR10201MATRIX METALLOPROTEINASEcoord: 155..318
score: 1.2E-110coord: 48..136
score: 1.2E-110coord: 5..25
score: 1.2E
NoneNo IPR availablePANTHERPTHR10201:SF140F20P5.11 PROTEINcoord: 48..136
score: 1.2E-110coord: 155..318
score: 1.2E-110coord: 5..25
score: 1.2E
NoneNo IPR availableunknownSSF55486Metalloproteases ("zincins"), catalytic domaincoord: 162..319
score: 3.42

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
CSPI05G28480Csa5G632110Cucumber (Chinese Long) v2cpicuB239
CSPI05G28480ClCG09G018600Watermelon (Charleston Gray)cpiwcgB356
CSPI05G28480CsaV3_5G037560Cucumber (Chinese Long) v3cpicucB284
CSPI05G28480Cucsa.158760Cucumber (Gy14) v1cgycpiB236
CSPI05G28480CmoCh15G003220Cucurbita moschata (Rifu)cmocpiB296
CSPI05G28480CsGy5G027810Cucumber (Gy14) v2cgybcpiB225
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
CSPI05G28480Melon (DHL92) v3.6.1cpimedB339
CSPI05G28480Melon (DHL92) v3.6.1cpimedB377
CSPI05G28480Cucumber (Gy14) v2cgybcpiB035
CSPI05G28480Silver-seed gourdcarcpiB0297
CSPI05G28480Silver-seed gourdcarcpiB0722
CSPI05G28480Cucumber (Chinese Long) v3cpicucB259
CSPI05G28480Watermelon (97103) v2cpiwmbB357
CSPI05G28480Watermelon (97103) v2cpiwmbB429
CSPI05G28480Wax gourdcpiwgoB473
CSPI05G28480Wax gourdcpiwgoB505
CSPI05G28480Wild cucumber (PI 183967)cpicpiB036
CSPI05G28480Cucumber (Gy14) v1cgycpiB110
CSPI05G28480Cucurbita maxima (Rimu)cmacpiB304
CSPI05G28480Cucurbita maxima (Rimu)cmacpiB730
CSPI05G28480Cucurbita moschata (Rifu)cmocpiB216
CSPI05G28480Cucurbita moschata (Rifu)cmocpiB722
CSPI05G28480Cucumber (Chinese Long) v2cpicuB219
CSPI05G28480Melon (DHL92) v3.5.1cpimeB349
CSPI05G28480Melon (DHL92) v3.5.1cpimeB382
CSPI05G28480Watermelon (Charleston Gray)cpiwcgB384
CSPI05G28480Watermelon (Charleston Gray)cpiwcgB360
CSPI05G28480Watermelon (97103) v1cpiwmB370
CSPI05G28480Watermelon (97103) v1cpiwmB432
CSPI05G28480Cucurbita pepo (Zucchini)cpecpiB177
CSPI05G28480Cucurbita pepo (Zucchini)cpecpiB414
CSPI05G28480Bottle gourd (USVL1VR-Ls)cpilsiB355
CSPI05G28480Bottle gourd (USVL1VR-Ls)cpilsiB352