CmoCh14G005110 (gene) Cucurbita moschata (Rifu)

Overview
NameCmoCh14G005110
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu))
DescriptionWerner Syndrome-like exonuclease
LocationCmo_Chr14 : 2529015 .. 2529644 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGCTCACCATCGTTGACCGTGAAGATCCCTCCGGTTCCCACAATTACTTCGACGTTACTTTCGAATCCGATGAGCCGATTCTCACTTTGCTCACCATTTCACCACTCATGGTAGACGATTGGATTTCAGAAACCCTCAACACTCAAACTTCCCCTCTCATCGTCGGCCTCGACATCGAGTGGCGCCCTAACATTCGCTCCTACGAGAACCCCGTGGCCACGTTACAACTCTGCACCGGCCGCCGATGCCTGATTGTGCAACTGATTTACGCACCTGAATTCCCTAAATCTCTGTATGAGTTTCTGGAAAACGAATCCATCAGTTTTGTAGGAGTCGGAATCGAGGGGGACGCTGAAAAACTGAACTGTGACTACGGATTGAAAGTGAGGAAGACGGTGGATCTGAGGAATTTGGCTGAGAGCGTTACGGGAAGAAGAGAATTGAAGAATGCGGGATTGAAGAGTCTGGCGAAAGAGATTTTGGGGAAAGAGATCCGAAAGCCGAGGGGCGTGACGTTGAGTAGGTGGGATCAAGAATGGCTTACTCATAATCAGATTAAGTATGCTTGTTTTGATGCGTTTGTGTCGTATGAGATTGGAAGGCATTTGCAAGCATCATCCAGTTGA

mRNA sequence

ATGGCGCTCACCATCGTTGACCGTGAAGATCCCTCCGGTTCCCACAATTACTTCGACGTTACTTTCGAATCCGATGAGCCGATTCTCACTTTGCTCACCATTTCACCACTCATGGTAGACGATTGGATTTCAGAAACCCTCAACACTCAAACTTCCCCTCTCATCGTCGGCCTCGACATCGAGTGGCGCCCTAACATTCGCTCCTACGAGAACCCCGTGGCCACGTTACAACTCTGCACCGGCCGCCGATGCCTGATTGTGCAACTGATTTACGCACCTGAATTCCCTAAATCTCTGTATGAGTTTCTGGAAAACGAATCCATCAGTTTTGTAGGAGTCGGAATCGAGGGGGACGCTGAAAAACTGAACTGTGACTACGGATTGAAAGTGAGGAAGACGGTGGATCTGAGGAATTTGGCTGAGAGCGTTACGGGAAGAAGAGAATTGAAGAATGCGGGATTGAAGAGTCTGGCGAAAGAGATTTTGGGGAAAGAGATCCGAAAGCCGAGGGGCGTGACGTTGAGTAGGTGGGATCAAGAATGGCTTACTCATAATCAGATTAAGTATGCTTGTTTTGATGCGTTTGTGTCGTATGAGATTGGAAGGCATTTGCAAGCATCATCCAGTTGA

Coding sequence (CDS)

ATGGCGCTCACCATCGTTGACCGTGAAGATCCCTCCGGTTCCCACAATTACTTCGACGTTACTTTCGAATCCGATGAGCCGATTCTCACTTTGCTCACCATTTCACCACTCATGGTAGACGATTGGATTTCAGAAACCCTCAACACTCAAACTTCCCCTCTCATCGTCGGCCTCGACATCGAGTGGCGCCCTAACATTCGCTCCTACGAGAACCCCGTGGCCACGTTACAACTCTGCACCGGCCGCCGATGCCTGATTGTGCAACTGATTTACGCACCTGAATTCCCTAAATCTCTGTATGAGTTTCTGGAAAACGAATCCATCAGTTTTGTAGGAGTCGGAATCGAGGGGGACGCTGAAAAACTGAACTGTGACTACGGATTGAAAGTGAGGAAGACGGTGGATCTGAGGAATTTGGCTGAGAGCGTTACGGGAAGAAGAGAATTGAAGAATGCGGGATTGAAGAGTCTGGCGAAAGAGATTTTGGGGAAAGAGATCCGAAAGCCGAGGGGCGTGACGTTGAGTAGGTGGGATCAAGAATGGCTTACTCATAATCAGATTAAGTATGCTTGTTTTGATGCGTTTGTGTCGTATGAGATTGGAAGGCATTTGCAAGCATCATCCAGTTGA
Homology
[Close]
BLAST of CmoCh14G005110 vs. Swiss-Prot
Match: WEX_ARATH (Werner Syndrome-like exonuclease OS=Arabidopsis thaliana GN=WEX PE=1 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 1.6e-21
Identity = 58/165 (35.15%), Postives = 92/165 (55.76%), Query Frame = 1 

Query: 43  ISETLNTQTSPLIVGLDIEWRPNIRSYENP--VATLQLCTGRRCLIVQLIYAPEFPKSLY 102
           + +T   ++    VGLDIEWRP+ R    P  VAT+Q+C       V  I+    P+SL 
Sbjct: 117 VLDTKRDESGIAFVGLDIEWRPSFRKGVLPGKVATVQICVDSNYCDVMHIFHSGIPQSLQ 176

Query: 103 EFLENESISFVGVGIEGDAEKLNCDYGLKVRKTVDLRNLAESVTGRRELKNAGLKSLAKE 162
             +E+ ++  VG+GI+GD+ KL  DYG+ ++   DL +LA    G    K  GL SL + 
Sbjct: 177 HLIEDSTLVKVGIGIDGDSVKLFHDYGVSIKDVEDLSDLANQKIGGD--KKWGLASLTET 236

Query: 163 ILGKEIRKPRGVTLSRWDQEWLTHNQIKYACFDAFVSYEIGRHLQ 206
           ++ KE+ KP  + L  W+   L+  Q++YA  DA+ S+ + + L+
Sbjct: 237 LVCKELLKPNRIRLGNWEFYPLSKQQLQYAATDAYASWHLYKVLK 279

[Close]
BLAST of CmoCh14G005110 vs. Swiss-Prot
Match: WRN_HUMAN (Werner syndrome ATP-dependent helicase OS=Homo sapiens GN=WRN PE=1 SV=2)

HSP 1 Score: 92.0 bits (227), Expect = 8.0e-18
Identity = 63/191 (32.98%), Postives = 95/191 (49.74%), Query Frame = 1 

Query: 22  FESDEPILTLLTISPLMVD----DWISETLNTQTSPL-IVGLDIEWRPNI-RSYENPVAT 81
           FE D P L          D     ++SE ++   S   +VG D+EW P   R     VA 
Sbjct: 40  FEDDLPFLEFTGSIVYSYDASDCSFLSEDISMSLSDGDVVGFDMEWPPLYNRGKLGKVAL 99

Query: 82  LQLCTGR-RCLIVQLIYAPEFPKSLYEFLENESISFVGVGIEGDAEKLNCDYGLKVRKTV 141
           +QLC    +C +  +     FP+ L   LEN+++   GVGIEGD  KL  D+ +K++  V
Sbjct: 100 IQLCVSESKCYLFHVSSMSVFPQGLKMLLENKAVKKAGVGIEGDQWKLLRDFDIKLKNFV 159

Query: 142 DLRNLAESVTGRRELKNAGLKSLAKEILGKEIRKPRGVTLSRWDQEWLTHNQIKYACFDA 201
           +L ++A       E     L SL K +LGK++ K + +  S W +  LT +Q  YA  DA
Sbjct: 160 ELTDVANKKLKCTE--TWSLNSLVKHLLGKQLLKDKSIRCSNWSKFPLTEDQKLYAATDA 219

Query: 202 FVSYEIGRHLQ 206
           +  + I R+L+
Sbjct: 220 YAGFIIYRNLE 228

[Close]
BLAST of CmoCh14G005110 vs. Swiss-Prot
Match: EXD2_HUMAN (Exonuclease 3'-5' domain-containing protein 2 OS=Homo sapiens GN=EXD2 PE=1 SV=2)

HSP 1 Score: 85.1 bits (209), Expect = 9.8e-16
Identity = 60/180 (33.33%), Postives = 91/180 (50.56%), Query Frame = 1 

Query: 31  LLTISPLMVDDWISETLNTQTSPL-IVGLDIEWRPNIRSYENPVATLQLCT-GRRCLIVQ 90
           ++T+S     D I   L ++     ++G+D EW  N+    +P++ LQ+ +    C++V+
Sbjct: 79  VVTVSQEAEWDQIEPLLRSELEDFPVLGIDCEW-VNLEGKASPLSLLQMASPSGLCVLVR 138

Query: 91  L----IYAPEFPKSLYEFLENESISFVGVGIEGDAEKLNCDYGLKVRKTVDLRNLAESVT 150
           L          P++L + L + +I  VGVG   DA KL  DYGL VR  +DLR LA    
Sbjct: 139 LPKLICGGKTLPRTLLDILADGTILKVGVGCSEDASKLLQDYGLVVRGCLDLRYLAMRQR 198

Query: 151 GRRELKNAGLKSLAKEILGKEIRKPRGVTLSRWDQEWLTHNQIKYACFDAFVSYEIGRHL 205
                    LKSLA+ +L   + K   +  S WD E LT +Q+ YA  DA +S  +  HL
Sbjct: 199 NNLLCNGLSLKSLAETVLNFPLDKSLLLRCSNWDAETLTEDQVIYAARDAQISVALFLHL 257

[Close]
BLAST of CmoCh14G005110 vs. Swiss-Prot
Match: EXD2_MOUSE (Exonuclease 3'-5' domain-containing protein 2 OS=Mus musculus GN=Exd2 PE=1 SV=2)

HSP 1 Score: 84.7 bits (208), Expect = 1.3e-15
Identity = 58/155 (37.42%), Postives = 82/155 (52.90%), Query Frame = 1 

Query: 55  IVGLDIEWRPNIRSYENPVATLQLCTGRR-CLIVQL---IYAPE-FPKSLYEFLENESIS 114
           ++G+D EW  N+    +P++ LQ+ +    C +V+L   IY     P++L + L + +I 
Sbjct: 133 VLGIDCEW-VNLEGKASPLSLLQMASPSGFCALVRLPRLIYGGRTLPRTLLDILADGAIL 192

Query: 115 FVGVGIEGDAEKLNCDYGLKVRKTVDLRNLAESVTGRRELKNAGLKSLAKEILGKEIRKP 174
            VGVG   DA KL  DYGL VR  +DLR LA             LKSLA+ IL   + K 
Sbjct: 193 KVGVGCSEDANKLLQDYGLIVRGCLDLRYLAMKQGNNILCNGLSLKSLAETILNFPLDKS 252

Query: 175 RGVTLSRWDQEWLTHNQIKYACFDAFVSYEIGRHL 205
             +  S WD E LT +Q+ YA  DA +S  +  HL
Sbjct: 253 LLLRCSNWDAENLTEDQVTYAARDAQISVALFLHL 286

[Close]
BLAST of CmoCh14G005110 vs. Swiss-Prot
Match: WRN_XENLA (Werner syndrome ATP-dependent helicase homolog OS=Xenopus laevis GN=wrn PE=2 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 1.3e-15
Identity = 51/153 (33.33%), Postives = 77/153 (50.33%), Query Frame = 1 

Query: 55  IVGLDIEWRP-NIRSYENPVATLQLCTG-RRCLIVQLIYAPEFPKSLYEFLENESISFVG 114
           ++G DIEW P   +     VA +Q+C   ++C +  +     FPK L   LE+ES+  VG
Sbjct: 72  VLGFDIEWPPVYTKGKTGKVALIQVCVSEKKCYLFHISPMAGFPKGLKRLLEDESVRKVG 131

Query: 115 VGIEGDAEKLNCDYGLKVRKTVDLRNLAESVTGRRELKNAGLKSLAKEILGKEIRKPRGV 174
           VGIEGD  KL  DY LK++  ++L  +A      +E        L K +  +++ K +  
Sbjct: 132 VGIEGDQWKLMSDYELKLKGFIELSEMANQKLRCKE--KWTFNGLIKHLFKEQLYKRKSY 191

Query: 175 TLSRWDQEWLTHNQIKYACFDAFVSYEIGRHLQ 206
             S WD   LT +Q  YA  DA+    I + L+
Sbjct: 192 RCSNWDIFLLTEDQKLYAATDAYAGLLIYKKLE 222

[Close]
BLAST of CmoCh14G005110 vs. TrEMBL
Match: E5GBP0_CUCME (3'-5' exonuclease OS=Cucumis melo subsp. melo PE=4 SV=1)

HSP 1 Score: 340.5 bits (872), Expect = 1.4e-90
Identity = 161/209 (77.03%), Postives = 183/209 (87.56%), Query Frame = 1 

Query: 1   MALTIVDREDPSGSHNYFDVTFESDEPILTLLTISPLMVDDWISETLNTQTSPLIVGLDI 60
           M++TIVD E PS SHNYFD+TF++DEPILTLLT SP MVDDWIS+TL  QT PL+VGLDI
Sbjct: 1   MSITIVDHEVPSDSHNYFDITFDNDEPILTLLTTSPSMVDDWISQTLTIQTPPLLVGLDI 60

Query: 61  EWRPNIRSYENPVATLQLCTGRRCLIVQLIYAPEFPKSLYEFLENESISFVGVGIEGDAE 120
           EWRPN RSY+NPVATLQLC GRRCLI+QL++ PE PKSL+EFLENES +FVGVGI+ DAE
Sbjct: 61  EWRPNNRSYDNPVATLQLCIGRRCLILQLLHIPEIPKSLFEFLENESFTFVGVGIDEDAE 120

Query: 121 KLNCDYGLKVRKTVDLRNLAESVTGRRELKNAGLKSLAKEILGKEIRKPRGVTLSRWDQE 180
           KLNCDYGLKV K +DLR+LAESVTGR ELKNAGLK L KE+LGKEI KP+ VT+SRWDQE
Sbjct: 121 KLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQE 180

Query: 181 WLTHNQIKYACFDAFVSYEIGRHLQASSS 210
           WLT NQ+KYAC DAF S+EIGR LQ+ SS
Sbjct: 181 WLTLNQVKYACIDAFFSFEIGRFLQSESS 209

[Close]
BLAST of CmoCh14G005110 vs. TrEMBL
Match: A0A0A0LBN3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G819870 PE=4 SV=1)

HSP 1 Score: 335.9 bits (860), Expect = 3.6e-89
Identity = 159/208 (76.44%), Postives = 182/208 (87.50%), Query Frame = 1 

Query: 1   MALTIVDREDPSGSHNYFDVTFESDEPILTLLTISPLMVDDWISETLNTQTSPLIVGLDI 60
           MA+TIVD E PS SHNYFD+TF++DEPILTLLT SP MVD WIS+TL  QT PL+VGLDI
Sbjct: 1   MAITIVDHEVPSDSHNYFDITFDTDEPILTLLTTSPSMVDGWISQTLAIQTPPLLVGLDI 60

Query: 61  EWRPNIRSYENPVATLQLCTGRRCLIVQLIYAPEFPKSLYEFLENESISFVGVGIEGDAE 120
           EWRPN RSY+NPVATLQLC GRRCLI+QL++ PE PKSL+EFLENES +FVGVGI+ DAE
Sbjct: 61  EWRPNNRSYDNPVATLQLCIGRRCLILQLLHMPEIPKSLFEFLENESFTFVGVGIDEDAE 120

Query: 121 KLNCDYGLKVRKTVDLRNLAESVTGRRELKNAGLKSLAKEILGKEIRKPRGVTLSRWDQE 180
           KLNCDYGLKV K +DLR+LAESVTGR ELKNAGLK L KE+LGKEI KP+ VT+SRWDQE
Sbjct: 121 KLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQE 180

Query: 181 WLTHNQIKYACFDAFVSYEIGRHLQASS 209
           WLT NQ+KYAC DAF S+EIG+ LQ++S
Sbjct: 181 WLTLNQVKYACIDAFFSFEIGKFLQSAS 208

[Close]
BLAST of CmoCh14G005110 vs. TrEMBL
Match: B9SNW3_RICCO (3-5 exonuclease, putative OS=Ricinus communis GN=RCOM_0582440 PE=4 SV=1)

HSP 1 Score: 238.0 bits (606), Expect = 1.0e-59
Identity = 121/210 (57.62%), Postives = 151/210 (71.90%), Query Frame = 1 

Query: 1   MALTIVDREDPSGSHNYFDVTFESDEPILTLLTISPLMVDDWISETL--NTQTSPLIVGL 60
           M L I D E  + +HN +D+TF +D+ I TL+T SP +V+ W+ ET   N QT P IVGL
Sbjct: 1   MTLGIRDYELINDTHNLYDITFFTDQ-IHTLVTHSPSLVEQWLIETQGQNNQTQPTIVGL 60

Query: 61  DIEWRPNI-RSYENPVATLQLCTGRRCLIVQLIYAPEFPKSLYEFLENESISFVGVGIEG 120
           D+EWRPN  R  ENPVATLQLC G RCLI QLI++P  P+SL++FL+N S  F GVGIE 
Sbjct: 61  DVEWRPNFSRHIENPVATLQLCIGSRCLIYQLIHSPRIPQSLFDFLKNSSYVFAGVGIES 120

Query: 121 DAEKLNCDYGLKVRKTVDLRNLAESVTGRRELKNAGLKSLAKEILGKEIRKPRGVTLSRW 180
           D EKL  DYGL V   ++LR +A    G +ELKNAGLK L K++LGKEI+KP+ VT+SRW
Sbjct: 121 DVEKLVEDYGLSVGNVMELRRVAAESLGVKELKNAGLKELVKQVLGKEIQKPKRVTMSRW 180

Query: 181 DQEWLTHNQIKYACFDAFVSYEIGRHLQAS 208
           D  WL+H+Q++YAC DAFV  EIGR L  S
Sbjct: 181 DSMWLSHDQVQYACLDAFVCSEIGRILTQS 209

[Close]
BLAST of CmoCh14G005110 vs. TrEMBL
Match: A0A0A0LHC5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G819830 PE=4 SV=1)

HSP 1 Score: 235.0 bits (598), Expect = 8.6e-59
Identity = 121/206 (58.74%), Postives = 149/206 (72.33%), Query Frame = 1 

Query: 3   LTIVDREDPSGSHNYFDVTFESDEPILTLLTISPLMVDDWISETLNT---QTSPLIVGLD 62
           LTI DR+ P  SH+Y+D+TF+ DE ILTL+T SP MVD WISE L     +   L+VGLD
Sbjct: 4   LTISDRQIPLNSHDYYDITFDDDE-ILTLVTASPSMVDMWISEILRIHRRRLHRLVVGLD 63

Query: 63  IEWRPNI-RSYENPVATLQLCTGRRCLIVQLIYAPEFPKSLYEFLENESISFVGVGIEGD 122
           +EWRPN  R + NPVATLQLC GRRCLI QLI+A E P+SL +FLE+++ +FVGVGI+ D
Sbjct: 64  VEWRPNFDRHFRNPVATLQLCVGRRCLIFQLIHASETPQSLIDFLEDDTFTFVGVGIDND 123

Query: 123 AEKLNCDYGLKVRKTVDLRNLAESVTGRRELKNAGLKSLAKEILGKEIRKPRGVTLSRWD 182
             KL  DY L V  TVDLR LA       E + AGLK+L +E+LG+EI KPR V LSRWD
Sbjct: 124 VLKLYNDYDLNVANTVDLRELAADEMQSDEFRTAGLKTLGREVLGREIDKPRNVKLSRWD 183

Query: 183 QEWLTHNQIKYACFDAFVSYEIGRHL 205
           ++WL   QI YA  DAF+S+EIGR+L
Sbjct: 184 RQWLNPAQILYATVDAFLSFEIGRYL 208

[Close]
BLAST of CmoCh14G005110 vs. TrEMBL
Match: M1CVH6_SOLTU (Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG400029410 PE=4 SV=1)

HSP 1 Score: 224.2 bits (570), Expect = 1.5e-55
Identity = 112/212 (52.83%), Postives = 143/212 (67.45%), Query Frame = 1 

Query: 1   MALTIVDREDPSGSHNYFDVTFESDEPILTLLTISPLMVDDWISETLNTQTSP--LIVGL 60
           M L IVD+E+    ++ FDV F SD  I T++T  P ++  WISET ++ +    LI GL
Sbjct: 1   MNLRIVDKEEQDNRYDLFDVHFHSDV-IKTMVTPDPDIITQWISETESSHSGNRRLIAGL 60

Query: 61  DIEWRPNI-RSYENPVATLQLCTGRRCLIVQLIYAPEFPKSLYEFLENESISFVGVGIEG 120
           D+EWRPN  R+ +NPVA LQ+C  RRCLI+QL+Y    P+SL+ FL N    FVG+GIE 
Sbjct: 61  DVEWRPNFHRNQQNPVAVLQICVDRRCLIIQLLYCRSIPESLFNFLGNREYDFVGIGIES 120

Query: 121 DAEKLNCDYGLKVRKTVDLRNLAESVTGRRELKNAGLKSLAKEILGKEIRKPRGVTLSRW 180
           D EKL  DY L V+  VDLR +A  V G + LKNAGLK L   +LGKEI KP+ VT+ RW
Sbjct: 121 DVEKLLEDYELNVKNVVDLRGIAADVYGMKNLKNAGLKELCNVVLGKEIVKPKSVTMGRW 180

Query: 181 DQEWLTHNQIKYACFDAFVSYEIGRHLQASSS 210
           D EWL+ NQ++YAC DAFVS EI RHL   ++
Sbjct: 181 DSEWLSLNQVQYACLDAFVSSEIARHLNVGAA 211

[Close]
BLAST of CmoCh14G005110 vs. TAIR10
Match: AT4G13870.2 (AT4G13870.2 Werner syndrome-like exonuclease)

HSP 1 Score: 104.4 bits (259), Expect = 8.8e-23
Identity = 58/165 (35.15%), Postives = 92/165 (55.76%), Query Frame = 1 

Query: 43  ISETLNTQTSPLIVGLDIEWRPNIRSYENP--VATLQLCTGRRCLIVQLIYAPEFPKSLY 102
           + +T   ++    VGLDIEWRP+ R    P  VAT+Q+C       V  I+    P+SL 
Sbjct: 117 VLDTKRDESGIAFVGLDIEWRPSFRKGVLPGKVATVQICVDSNYCDVMHIFHSGIPQSLQ 176

Query: 103 EFLENESISFVGVGIEGDAEKLNCDYGLKVRKTVDLRNLAESVTGRRELKNAGLKSLAKE 162
             +E+ ++  VG+GI+GD+ KL  DYG+ ++   DL +LA    G    K  GL SL + 
Sbjct: 177 HLIEDSTLVKVGIGIDGDSVKLFHDYGVSIKDVEDLSDLANQKIGGD--KKWGLASLTET 236

Query: 163 ILGKEIRKPRGVTLSRWDQEWLTHNQIKYACFDAFVSYEIGRHLQ 206
           ++ KE+ KP  + L  W+   L+  Q++YA  DA+ S+ + + L+
Sbjct: 237 LVCKELLKPNRIRLGNWEFYPLSKQQLQYAATDAYASWHLYKVLK 279

[Close]
BLAST of CmoCh14G005110 vs. TAIR10
Match: AT2G36110.1 (AT2G36110.1 Polynucleotidyl transferase, ribonuclease H-like superfamily protein)

HSP 1 Score: 98.2 bits (243), Expect = 6.3e-21
Identity = 55/151 (36.42%), Postives = 86/151 (56.95%), Query Frame = 1 

Query: 53  PLIVGLDIEWRPNIRSYENPVATLQLCTGRRCLIVQLIYAPEFPKSLYEFLENESISFVG 112
           PL+VGLD++W P     + P   LQLC G RCLI+QL +    P+ L  FLE+E+I+FVG
Sbjct: 55  PLVVGLDVQWTPG--GSDPPPDILQLCVGNRCLIIQLSHCKRIPEVLRSFLEDETITFVG 114

Query: 113 VGIEGDAEKL-NCDYGLKVRKTVDLRNLAESVTGRRELKNAGLKSLAKEILG-KEIRKPR 172
           V    D  KL    + L++ + +D+R+   +      L N+  + + +E LG K +RK +
Sbjct: 115 VWNSQDQGKLERFRHQLEIWRLLDIRHYLPT-----RLLNSSFEKIVEECLGYKGVRKDK 174

Query: 173 GVTLSRWDQEWLTHNQIKYACFDAFVSYEIG 202
            + +S W    L+H+QI  A  D +V  ++G
Sbjct: 175 EICMSNWGARSLSHDQIVQASDDVYVCCKLG 198

[Close]
BLAST of CmoCh14G005110 vs. TAIR10
Match: AT3G12470.1 (AT3G12470.1 Polynucleotidyl transferase, ribonuclease H-like superfamily protein)

HSP 1 Score: 96.7 bits (239), Expect = 1.8e-20
Identity = 62/193 (32.12%), Postives = 99/193 (51.30%), Query Frame = 1 

Query: 14  SHNYFDVTFESDEPILTLLTISPLMVDDWI----SETLNTQTSPLIVGLDIEWRPNIRSY 73
           +H    V F  D+ I+T+ T +  ++  WI    S   N    PL+VG+ ++WRP+    
Sbjct: 13  THQKHFVDFFGDDLIVTV-TPTASVIRRWIRSVRSYNRNHSVHPLVVGIGVQWRPDSSDP 72

Query: 74  ENPVATLQLCTGRRCLIVQLIYAPEFPKSLYEFLENESISFVGVGIEGDAEKL-NCDYGL 133
           + P  TLQLC G RCLI+QL Y    PK L  FL +   +FVGV    D +KL  C + +
Sbjct: 73  DPPPKTLQLCVGSRCLIIQLGYNYGLPKVLRTFLADPKTTFVGVWNGQDQKKLEKCRHRV 132

Query: 134 KVRKTVDLRNLAESVTGRRELKNAGLKSLAKEILGK-EIRKPRGVTLSRWDQEWLTHNQI 193
           ++ K +D+R       G R +     + + KE LG+  +R    + +S W    L H Q+
Sbjct: 133 EIGKLLDIRMFVRDSRGAR-MCFCSFEQIVKERLGRVGVRLDPAICMSDWGVYNLNHYQV 192

Query: 194 KYACFDAFVSYEI 201
             A  +++V +++
Sbjct: 193 LQASIESYVCFKL 203

[Close]
BLAST of CmoCh14G005110 vs. TAIR10
Match: AT3G12410.1 (AT3G12410.1 Polynucleotidyl transferase, ribonuclease H-like superfamily protein)

HSP 1 Score: 94.7 bits (234), Expect = 7.0e-20
Identity = 68/211 (32.23%), Postives = 108/211 (51.18%), Query Frame = 1 

Query: 14  SHNYFDVTFESDEPILTLLTISPLMVDDWISETL-NTQTS--PLIVGLDIEW-------- 73
           +H  + V F  DE I+T+ T    ++  WI   L N + S  PL+VG+ ++W        
Sbjct: 14  THLEYSVDFFGDEFIVTV-TWDSSVISRWIRNVLFNNRFSSHPLVVGVGVQWTPFSYYSD 73

Query: 74  -RPN--------IRSY-ENPVATLQLCTGRRCLIVQLIYAPEFPKSLYEFLENESISFVG 133
            RPN        IR Y +NP   LQLC G RCLI+QL Y  + P +L  FL +   +FVG
Sbjct: 74  PRPNNYYADPPPIRYYSDNPADILQLCVGNRCLIIQLGYCDQVPNNLRSFLADPETTFVG 133

Query: 134 VGIEGDAEKL-NCDYGLKVRKTVDLRNLAESVTGRRELKNAGLKSLAKEILGKE-IRKPR 193
           V    DA KL  C + L++ + +D+R       G R ++ +  + + +E +G + +    
Sbjct: 134 VWNGQDAGKLARCCHQLEIGELLDIRRYVTDSWG-RSMRRSSFEEIVEECMGYQGVMLDP 193

Query: 194 GVTLSRWDQEWLTHNQIKYACFDAFVSYEIG 202
            +++S W    L  +QI  A  DA+V +++G
Sbjct: 194 EISMSDWTAYDLDLDQILQASLDAYVCHQLG 222

[Close]
BLAST of CmoCh14G005110 vs. TAIR10
Match: AT3G12430.1 (AT3G12430.1 Polynucleotidyl transferase, ribonuclease H-like superfamily protein)

HSP 1 Score: 84.0 bits (206), Expect = 1.2e-16
Identity = 56/202 (27.72%), Postives = 91/202 (45.05%), Query Frame = 1 

Query: 26  EPILTLLTISPLMVDDWISETLNTQ---TSPLIVGLDIEWRPNI---------------- 85
           E  + ++T  P ++  WI + L+     + PL+VG+ ++W P+                 
Sbjct: 24  EEFIVIVTPDPSVIGQWIHDVLSHNRFSSHPLVVGVGVQWTPSSYYSASSPVRYRSDSPP 83

Query: 86  -----------RSYENPVATLQLCTGRRCLIVQLIYAPEFPKSLYEFLENESISFVGVGI 145
                      R ++ P  TLQLC G RC+I+QL +    P+ L  FL +   +FVG+  
Sbjct: 84  VRYRSDSPRVRRRFDPPADTLQLCVGNRCIIIQLSHCERVPQVLRNFLADRDYTFVGIWN 143

Query: 146 EGDAEKL-NCDYGLKVRKTVDLRNLAESVTGRRELKNAGLKSLAKEILGKE-IRKPRGVT 196
             DA KL    + L++   +DLR      +G R +K    + + +E LG   +R  R V+
Sbjct: 144 SQDAGKLKRSKHELEIAVLLDLRKFVSDSSG-RTMKRCSFEKIVEENLGHSGVRLDRKVS 203

[Close]
BLAST of CmoCh14G005110 vs. NCBI nr
Match: gi|659085101|ref|XP_008443244.1| (PREDICTED: Werner Syndrome-like exonuclease [Cucumis melo])

HSP 1 Score: 340.5 bits (872), Expect = 2.1e-90
Identity = 161/209 (77.03%), Postives = 183/209 (87.56%), Query Frame = 1 

Query: 1   MALTIVDREDPSGSHNYFDVTFESDEPILTLLTISPLMVDDWISETLNTQTSPLIVGLDI 60
           M++TIVD E PS SHNYFD+TF++DEPILTLLT SP MVDDWIS+TL  QT PL+VGLDI
Sbjct: 1   MSITIVDHEVPSDSHNYFDITFDNDEPILTLLTTSPSMVDDWISQTLTIQTPPLLVGLDI 60

Query: 61  EWRPNIRSYENPVATLQLCTGRRCLIVQLIYAPEFPKSLYEFLENESISFVGVGIEGDAE 120
           EWRPN RSY+NPVATLQLC GRRCLI+QL++ PE PKSL+EFLENES +FVGVGI+ DAE
Sbjct: 61  EWRPNNRSYDNPVATLQLCIGRRCLILQLLHIPEIPKSLFEFLENESFTFVGVGIDEDAE 120

Query: 121 KLNCDYGLKVRKTVDLRNLAESVTGRRELKNAGLKSLAKEILGKEIRKPRGVTLSRWDQE 180
           KLNCDYGLKV K +DLR+LAESVTGR ELKNAGLK L KE+LGKEI KP+ VT+SRWDQE
Sbjct: 121 KLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQE 180

Query: 181 WLTHNQIKYACFDAFVSYEIGRHLQASSS 210
           WLT NQ+KYAC DAF S+EIGR LQ+ SS
Sbjct: 181 WLTLNQVKYACIDAFFSFEIGRFLQSESS 209

[Close]
BLAST of CmoCh14G005110 vs. NCBI nr
Match: gi|778685161|ref|XP_011652174.1| (PREDICTED: Werner Syndrome-like exonuclease [Cucumis sativus])

HSP 1 Score: 335.9 bits (860), Expect = 5.1e-89
Identity = 159/208 (76.44%), Postives = 182/208 (87.50%), Query Frame = 1 

Query: 1   MALTIVDREDPSGSHNYFDVTFESDEPILTLLTISPLMVDDWISETLNTQTSPLIVGLDI 60
           MA+TIVD E PS SHNYFD+TF++DEPILTLLT SP MVD WIS+TL  QT PL+VGLDI
Sbjct: 1   MAITIVDHEVPSDSHNYFDITFDTDEPILTLLTTSPSMVDGWISQTLAIQTPPLLVGLDI 60

Query: 61  EWRPNIRSYENPVATLQLCTGRRCLIVQLIYAPEFPKSLYEFLENESISFVGVGIEGDAE 120
           EWRPN RSY+NPVATLQLC GRRCLI+QL++ PE PKSL+EFLENES +FVGVGI+ DAE
Sbjct: 61  EWRPNNRSYDNPVATLQLCIGRRCLILQLLHMPEIPKSLFEFLENESFTFVGVGIDEDAE 120

Query: 121 KLNCDYGLKVRKTVDLRNLAESVTGRRELKNAGLKSLAKEILGKEIRKPRGVTLSRWDQE 180
           KLNCDYGLKV K +DLR+LAESVTGR ELKNAGLK L KE+LGKEI KP+ VT+SRWDQE
Sbjct: 121 KLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQE 180

Query: 181 WLTHNQIKYACFDAFVSYEIGRHLQASS 209
           WLT NQ+KYAC DAF S+EIG+ LQ++S
Sbjct: 181 WLTLNQVKYACIDAFFSFEIGKFLQSAS 208

[Close]
BLAST of CmoCh14G005110 vs. NCBI nr
Match: gi|255573515|ref|XP_002527682.1| (PREDICTED: Werner Syndrome-like exonuclease [Ricinus communis])

HSP 1 Score: 238.0 bits (606), Expect = 1.5e-59
Identity = 121/210 (57.62%), Postives = 151/210 (71.90%), Query Frame = 1 

Query: 1   MALTIVDREDPSGSHNYFDVTFESDEPILTLLTISPLMVDDWISETL--NTQTSPLIVGL 60
           M L I D E  + +HN +D+TF +D+ I TL+T SP +V+ W+ ET   N QT P IVGL
Sbjct: 1   MTLGIRDYELINDTHNLYDITFFTDQ-IHTLVTHSPSLVEQWLIETQGQNNQTQPTIVGL 60

Query: 61  DIEWRPNI-RSYENPVATLQLCTGRRCLIVQLIYAPEFPKSLYEFLENESISFVGVGIEG 120
           D+EWRPN  R  ENPVATLQLC G RCLI QLI++P  P+SL++FL+N S  F GVGIE 
Sbjct: 61  DVEWRPNFSRHIENPVATLQLCIGSRCLIYQLIHSPRIPQSLFDFLKNSSYVFAGVGIES 120

Query: 121 DAEKLNCDYGLKVRKTVDLRNLAESVTGRRELKNAGLKSLAKEILGKEIRKPRGVTLSRW 180
           D EKL  DYGL V   ++LR +A    G +ELKNAGLK L K++LGKEI+KP+ VT+SRW
Sbjct: 121 DVEKLVEDYGLSVGNVMELRRVAAESLGVKELKNAGLKELVKQVLGKEIQKPKRVTMSRW 180

Query: 181 DQEWLTHNQIKYACFDAFVSYEIGRHLQAS 208
           D  WL+H+Q++YAC DAFV  EIGR L  S
Sbjct: 181 DSMWLSHDQVQYACLDAFVCSEIGRILTQS 209

[Close]
BLAST of CmoCh14G005110 vs. NCBI nr
Match: gi|449437986|ref|XP_004136771.1| (PREDICTED: Werner Syndrome-like exonuclease [Cucumis sativus])

HSP 1 Score: 235.0 bits (598), Expect = 1.2e-58
Identity = 121/206 (58.74%), Postives = 149/206 (72.33%), Query Frame = 1 

Query: 3   LTIVDREDPSGSHNYFDVTFESDEPILTLLTISPLMVDDWISETLNT---QTSPLIVGLD 62
           LTI DR+ P  SH+Y+D+TF+ DE ILTL+T SP MVD WISE L     +   L+VGLD
Sbjct: 4   LTISDRQIPLNSHDYYDITFDDDE-ILTLVTASPSMVDMWISEILRIHRRRLHRLVVGLD 63

Query: 63  IEWRPNI-RSYENPVATLQLCTGRRCLIVQLIYAPEFPKSLYEFLENESISFVGVGIEGD 122
           +EWRPN  R + NPVATLQLC GRRCLI QLI+A E P+SL +FLE+++ +FVGVGI+ D
Sbjct: 64  VEWRPNFDRHFRNPVATLQLCVGRRCLIFQLIHASETPQSLIDFLEDDTFTFVGVGIDND 123

Query: 123 AEKLNCDYGLKVRKTVDLRNLAESVTGRRELKNAGLKSLAKEILGKEIRKPRGVTLSRWD 182
             KL  DY L V  TVDLR LA       E + AGLK+L +E+LG+EI KPR V LSRWD
Sbjct: 124 VLKLYNDYDLNVANTVDLRELAADEMQSDEFRTAGLKTLGREVLGREIDKPRNVKLSRWD 183

Query: 183 QEWLTHNQIKYACFDAFVSYEIGRHL 205
           ++WL   QI YA  DAF+S+EIGR+L
Sbjct: 184 RQWLNPAQILYATVDAFLSFEIGRYL 208

[Close]
BLAST of CmoCh14G005110 vs. NCBI nr
Match: gi|764561966|ref|XP_011461409.1| (PREDICTED: Werner Syndrome-like exonuclease [Fragaria vesca subsp. vesca])

HSP 1 Score: 230.7 bits (587), Expect = 2.3e-57
Identity = 116/206 (56.31%), Postives = 145/206 (70.39%), Query Frame = 1 

Query: 1   MALTIVDREDPSGSHNYFDVTFESDEPILTLLTISPLMVDDWISETLNTQTSPLIVGLDI 60
           M ++IVD E P  +HN +DV+F  D+ I TL+T S  MV+ W+SE +       IVGLD+
Sbjct: 1   MTISIVDHELPYDTHNLYDVSFYDDQ-IHTLVTHSAPMVEAWLSEIIPIVNQNPIVGLDV 60

Query: 61  EWRPNIRSYENPVATLQLCTGRRCLIVQLIYAPEFPKSLYEFLENESISFVGVGIEGDAE 120
           EWRPN R+ +N VATLQLC   RCLI QL+YA   P++L++FL N S +FVG GIE D E
Sbjct: 61  EWRPNNRNSDNLVATLQLCVDHRCLIFQLLYAQTIPRALFDFLSNGSYTFVGAGIENDME 120

Query: 121 KLNCDYGLKVRKTVDLRNLAESVTGRRELKNAGLKSLAKEILGKEIRKPRGVTLSRWDQE 180
           KL  DY LKV  T+DL  LA +  G+ ELK AGLK L+KE+LGKE++KP  VT SRWD E
Sbjct: 121 KLLVDYDLKVTNTLDLGPLAATKLGKIELKKAGLKGLSKEVLGKELQKPSNVTRSRWDNE 180

Query: 181 WLTHNQIKYACFDAFVSYEIGRHLQA 207
           WL  NQ++YAC DAF+S EIGR L A
Sbjct: 181 WLMCNQVQYACIDAFISSEIGRRLTA 205

The following BLAST results are available for this feature:
BLAST of CmoCh14G005110 vs. Swiss-Prot
Match NameE-valueIdentityDescription
WEX_ARATH1.6e-2135.15Werner Syndrome-like exonuclease OS=Arabidopsis thaliana GN=WEX PE=1 SV=1[more]
WRN_HUMAN8.0e-1832.98Werner syndrome ATP-dependent helicase OS=Homo sapiens GN=WRN PE=1 SV=2[more]
EXD2_HUMAN9.8e-1633.33Exonuclease 3'-5' domain-containing protein 2 OS=Homo sapiens GN=EXD2 PE=1 SV=2[more]
EXD2_MOUSE1.3e-1537.42Exonuclease 3'-5' domain-containing protein 2 OS=Mus musculus GN=Exd2 PE=1 SV=2[more]
WRN_XENLA1.3e-1533.33Werner syndrome ATP-dependent helicase homolog OS=Xenopus laevis GN=wrn PE=2 SV=... [more]
BLAST of CmoCh14G005110 vs. TrEMBL
Match NameE-valueIdentityDescription
E5GBP0_CUCME1.4e-9077.033'-5' exonuclease OS=Cucumis melo subsp. melo PE=4 SV=1[more]
A0A0A0LBN3_CUCSA3.6e-8976.44Uncharacterized protein OS=Cucumis sativus GN=Csa_3G819870 PE=4 SV=1[more]
B9SNW3_RICCO1.0e-5957.623-5 exonuclease, putative OS=Ricinus communis GN=RCOM_0582440 PE=4 SV=1[more]
A0A0A0LHC5_CUCSA8.6e-5958.74Uncharacterized protein OS=Cucumis sativus GN=Csa_3G819830 PE=4 SV=1[more]
M1CVH6_SOLTU1.5e-5552.83Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG400029410 PE=4 SV=1[more]
BLAST of CmoCh14G005110 vs. TAIR10
Match NameE-valueIdentityDescription
AT4G13870.28.8e-2335.15 Werner syndrome-like exonuclease[more]
AT2G36110.16.3e-2136.42 Polynucleotidyl transferase, ribonuclease H-like superfamily protein[more]
AT3G12470.11.8e-2032.12 Polynucleotidyl transferase, ribonuclease H-like superfamily protein[more]
AT3G12410.17.0e-2032.23 Polynucleotidyl transferase, ribonuclease H-like superfamily protein[more]
AT3G12430.11.2e-1627.72 Polynucleotidyl transferase, ribonuclease H-like superfamily protein[more]
BLAST of CmoCh14G005110 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|659085101|ref|XP_008443244.1|2.1e-9077.03PREDICTED: Werner Syndrome-like exonuclease [Cucumis melo][more]
gi|778685161|ref|XP_011652174.1|5.1e-8976.44PREDICTED: Werner Syndrome-like exonuclease [Cucumis sativus][more]
gi|255573515|ref|XP_002527682.1|1.5e-5957.62PREDICTED: Werner Syndrome-like exonuclease [Ricinus communis][more]
gi|449437986|ref|XP_004136771.1|1.2e-5858.74PREDICTED: Werner Syndrome-like exonuclease [Cucumis sativus][more]
gi|764561966|ref|XP_011461409.1|2.3e-5756.31PREDICTED: Werner Syndrome-like exonuclease [Fragaria vesca subsp. vesca][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR0025623'-5'_exonuclease_dom
IPR012337RNaseH-like_sf
Vocabulary: Molecular Function
TermDefinition
GO:0003676nucleic acid binding
GO:00084083'-5' exonuclease activity
Vocabulary: Biological Process
TermDefinition
GO:0006139nucleobase-containing compound metabolic process
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0090305 nucleic acid phosphodiester bond hydrolysis
biological_process GO:0006139 nucleobase-containing compound metabolic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0008408 3'-5' exonuclease activity
molecular_function GO:0003676 nucleic acid binding
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh14G005110.1CmoCh14G005110.1mRNA


InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata
Date Performed: 2017-05-19
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR0025623'-5' exonuclease domainPFAMPF01612DNA_pol_A_exo1coord: 40..205
score: 1.2
IPR0025623'-5' exonuclease domainSMARTSM0047435exoneu6coord: 36..209
score: 7.
IPR012337Ribonuclease H-like domainGENE3DG3DSA:3.30.420.10coord: 15..205
score: 1.0
IPR012337Ribonuclease H-like domainunknownSSF53098Ribonuclease H-likecoord: 46..205
score: 3.24
NoneNo IPR availablePANTHERPTHR136203-5 EXONUCLEASEcoord: 1..205
score: 1.3