CmaCh14G005050 (gene) Cucurbita maxima (Rimu)

Overview
NameCmaCh14G005050
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionWerner Syndrome-like exonuclease
LocationCma_Chr14 : 2467628 .. 2468257 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGCTCACCATCGTTGAACGTGAAGATCCCTCCGGTTCCCACAATTACTTCGACGTTACTTTCGACTCCGATCAGCCGATTCTCACTTTGCTCACCACTTCACCATTCATGGTAGAGGATTGGATTGCAGAAACCCTCAACACTCAAACTTCACCTCTCGTCGTCGGCCTCGACATCGAGTGGCGCCCTAACAGTCGCTCCTACGAGAACCCCGTGGCCACGTTACAACTCTGCACCGGCAGCCGATGCCTGATTATGCAACTGATTTACACACCTGAATTCCCTATATCTCTGTATGAGTTTCTGGAAAACGAATCCATCAGTTTTGTAGGAGTCGGAATCGAGGATGACGCTGAAAAACTGAACTGTGACTACGGATTGAAAGTGAGGAAGACGGTCGATCTGAGGAATTTGGCTGAGAGGGTAACGGGAAGAAGAGAATTGAAGAATGCGGGATTGAAGAGTCTGGCGAAAGAGATTTTGGGGAAAGAGATCCGAAAGCCGAGGGGCGTGACGATGAGTAGGTGGGATCAAGAATGGCTTACTCATAATCAGATTAAGTATGCTTGTTTTGATGCGTTTGTGTCGTATGAGATTGGAAGGCATTTGCTATCATCATCCAGTTGA

mRNA sequence

ATGGCGCTCACCATCGTTGAACGTGAAGATCCCTCCGGTTCCCACAATTACTTCGACGTTACTTTCGACTCCGATCAGCCGATTCTCACTTTGCTCACCACTTCACCATTCATGGTAGAGGATTGGATTGCAGAAACCCTCAACACTCAAACTTCACCTCTCGTCGTCGGCCTCGACATCGAGTGGCGCCCTAACAGTCGCTCCTACGAGAACCCCGTGGCCACGTTACAACTCTGCACCGGCAGCCGATGCCTGATTATGCAACTGATTTACACACCTGAATTCCCTATATCTCTGTATGAGTTTCTGGAAAACGAATCCATCAGTTTTGTAGGAGTCGGAATCGAGGATGACGCTGAAAAACTGAACTGTGACTACGGATTGAAAGTGAGGAAGACGGTCGATCTGAGGAATTTGGCTGAGAGGGTAACGGGAAGAAGAGAATTGAAGAATGCGGGATTGAAGAGTCTGGCGAAAGAGATTTTGGGGAAAGAGATCCGAAAGCCGAGGGGCGTGACGATGAGTAGGTGGGATCAAGAATGGCTTACTCATAATCAGATTAAGTATGCTTGTTTTGATGCGTTTGTGTCGTATGAGATTGGAAGGCATTTGCTATCATCATCCAGTTGA

Coding sequence (CDS)

ATGGCGCTCACCATCGTTGAACGTGAAGATCCCTCCGGTTCCCACAATTACTTCGACGTTACTTTCGACTCCGATCAGCCGATTCTCACTTTGCTCACCACTTCACCATTCATGGTAGAGGATTGGATTGCAGAAACCCTCAACACTCAAACTTCACCTCTCGTCGTCGGCCTCGACATCGAGTGGCGCCCTAACAGTCGCTCCTACGAGAACCCCGTGGCCACGTTACAACTCTGCACCGGCAGCCGATGCCTGATTATGCAACTGATTTACACACCTGAATTCCCTATATCTCTGTATGAGTTTCTGGAAAACGAATCCATCAGTTTTGTAGGAGTCGGAATCGAGGATGACGCTGAAAAACTGAACTGTGACTACGGATTGAAAGTGAGGAAGACGGTCGATCTGAGGAATTTGGCTGAGAGGGTAACGGGAAGAAGAGAATTGAAGAATGCGGGATTGAAGAGTCTGGCGAAAGAGATTTTGGGGAAAGAGATCCGAAAGCCGAGGGGCGTGACGATGAGTAGGTGGGATCAAGAATGGCTTACTCATAATCAGATTAAGTATGCTTGTTTTGATGCGTTTGTGTCGTATGAGATTGGAAGGCATTTGCTATCATCATCCAGTTGA

Protein sequence

MALTIVEREDPSGSHNYFDVTFDSDQPILTLLTTSPFMVEDWIAETLNTQTSPLVVGLDIEWRPNSRSYENPVATLQLCTGSRCLIMQLIYTPEFPISLYEFLENESISFVGVGIEDDAEKLNCDYGLKVRKTVDLRNLAERVTGRRELKNAGLKSLAKEILGKEIRKPRGVTMSRWDQEWLTHNQIKYACFDAFVSYEIGRHLLSSSS
Homology
[Close]
BLAST of CmaCh14G005050 vs. Swiss-Prot
Match: WEX_ARATH (Werner Syndrome-like exonuclease OS=Arabidopsis thaliana GN=WEX PE=1 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 1.9e-19
Identity = 55/160 (34.38%), Postives = 89/160 (55.62%), Query Frame = 1 

Query: 43  IAETLNTQTSPLVVGLDIEWRPNSRSYENP--VATLQLCTGSRCLIMQLIYTPEFPISLY 102
           + +T   ++    VGLDIEWRP+ R    P  VAT+Q+C  S    +  I+    P SL 
Sbjct: 117 VLDTKRDESGIAFVGLDIEWRPSFRKGVLPGKVATVQICVDSNYCDVMHIFHSGIPQSLQ 176

Query: 103 EFLENESISFVGVGIEDDAEKLNCDYGLKVRKTVDLRNLAERVTGRRELKNAGLKSLAKE 162
             +E+ ++  VG+GI+ D+ KL  DYG+ ++   DL +LA +  G    K  GL SL + 
Sbjct: 177 HLIEDSTLVKVGIGIDGDSVKLFHDYGVSIKDVEDLSDLANQKIGGD--KKWGLASLTET 236

Query: 163 ILGKEIRKPRGVTMSRWDQEWLTHNQIKYACFDAFVSYEI 201
           ++ KE+ KP  + +  W+   L+  Q++YA  DA+ S+ +
Sbjct: 237 LVCKELLKPNRIRLGNWEFYPLSKQQLQYAATDAYASWHL 274

[Close]
BLAST of CmaCh14G005050 vs. Swiss-Prot
Match: WRN_HUMAN (Werner syndrome ATP-dependent helicase OS=Homo sapiens GN=WRN PE=1 SV=2)

HSP 1 Score: 89.7 bits (221), Expect = 4.0e-17
Identity = 55/152 (36.18%), Postives = 82/152 (53.95%), Query Frame = 1 

Query: 55  VVGLDIEWRP-NSRSYENPVATLQLCTG-SRCLIMQLIYTPEFPISLYEFLENESISFVG 114
           VVG D+EW P  +R     VA +QLC   S+C +  +     FP  L   LEN+++   G
Sbjct: 78  VVGFDMEWPPLYNRGKLGKVALIQLCVSESKCYLFHVSSMSVFPQGLKMLLENKAVKKAG 137

Query: 115 VGIEDDAEKLNCDYGLKVRKTVDLRNLAERVTGRRELKNAGLKSLAKEILGKEIRKPRGV 174
           VGIE D  KL  D+ +K++  V+L ++A +     E     L SL K +LGK++ K + +
Sbjct: 138 VGIEGDQWKLLRDFDIKLKNFVELTDVANKKLKCTE--TWSLNSLVKHLLGKQLLKDKSI 197

Query: 175 TMSRWDQEWLTHNQIKYACFDAFVSYEIGRHL 205
             S W +  LT +Q  YA  DA+  + I R+L
Sbjct: 198 RCSNWSKFPLTEDQKLYAATDAYAGFIIYRNL 227

[Close]
BLAST of CmaCh14G005050 vs. Swiss-Prot
Match: EXD2_HUMAN (Exonuclease 3'-5' domain-containing protein 2 OS=Homo sapiens GN=EXD2 PE=1 SV=2)

HSP 1 Score: 85.1 bits (209), Expect = 9.8e-16
Identity = 58/156 (37.18%), Postives = 83/156 (53.21%), Query Frame = 1 

Query: 55  VVGLDIEWRPNSRSYENPVATLQLCTGSR-CLIMQLIYT----PEFPISLYEFLENESIS 114
           V+G+D EW  N     +P++ LQ+ + S  C++++L          P +L + L + +I 
Sbjct: 104 VLGIDCEW-VNLEGKASPLSLLQMASPSGLCVLVRLPKLICGGKTLPRTLLDILADGTIL 163

Query: 115 FVGVGIEDDAEKLNCDYGLKVRKTVDLRNLAERVTGRRELKNAGLKSLAKEILGKEIRKP 174
            VGVG  +DA KL  DYGL VR  +DLR LA R           LKSLA+ +L   + K 
Sbjct: 164 KVGVGCSEDASKLLQDYGLVVRGCLDLRYLAMRQRNNLLCNGLSLKSLAETVLNFPLDKS 223

Query: 175 RGVTMSRWDQEWLTHNQIKYACFDAFVSYEIGRHLL 206
             +  S WD E LT +Q+ YA  DA +S  +  HLL
Sbjct: 224 LLLRCSNWDAETLTEDQVIYAARDAQISVALFLHLL 258

[Close]
BLAST of CmaCh14G005050 vs. Swiss-Prot
Match: EXD2_MOUSE (Exonuclease 3'-5' domain-containing protein 2 OS=Mus musculus GN=Exd2 PE=1 SV=2)

HSP 1 Score: 84.0 bits (206), Expect = 2.2e-15
Identity = 60/156 (38.46%), Postives = 84/156 (53.85%), Query Frame = 1 

Query: 55  VVGLDIEWRPNSRSYENPVATLQLCTGSR-CLIMQL---IYTPE-FPISLYEFLENESIS 114
           V+G+D EW  N     +P++ LQ+ + S  C +++L   IY     P +L + L + +I 
Sbjct: 133 VLGIDCEW-VNLEGKASPLSLLQMASPSGFCALVRLPRLIYGGRTLPRTLLDILADGAIL 192

Query: 115 FVGVGIEDDAEKLNCDYGLKVRKTVDLRNLAERVTGRRELKNAGLKSLAKEILGKEIRKP 174
            VGVG  +DA KL  DYGL VR  +DLR LA +           LKSLA+ IL   + K 
Sbjct: 193 KVGVGCSEDANKLLQDYGLIVRGCLDLRYLAMKQGNNILCNGLSLKSLAETILNFPLDKS 252

Query: 175 RGVTMSRWDQEWLTHNQIKYACFDAFVSYEIGRHLL 206
             +  S WD E LT +Q+ YA  DA +S  +  HLL
Sbjct: 253 LLLRCSNWDAENLTEDQVTYAARDAQISVALFLHLL 287

[Close]
BLAST of CmaCh14G005050 vs. Swiss-Prot
Match: WRN_XENLA (Werner syndrome ATP-dependent helicase homolog OS=Xenopus laevis GN=wrn PE=2 SV=1)

HSP 1 Score: 77.8 bits (190), Expect = 1.6e-13
Identity = 50/152 (32.89%), Postives = 75/152 (49.34%), Query Frame = 1 

Query: 55  VVGLDIEWRP-NSRSYENPVATLQLCTGSR-CLIMQLIYTPEFPISLYEFLENESISFVG 114
           V+G DIEW P  ++     VA +Q+C   + C +  +     FP  L   LE+ES+  VG
Sbjct: 72  VLGFDIEWPPVYTKGKTGKVALIQVCVSEKKCYLFHISPMAGFPKGLKRLLEDESVRKVG 131

Query: 115 VGIEDDAEKLNCDYGLKVRKTVDLRNLAERVTGRRELKNAGLKSLAKEILGKEIRKPRGV 174
           VGIE D  KL  DY LK++  ++L  +A +    +E        L K +  +++ K +  
Sbjct: 132 VGIEGDQWKLMSDYELKLKGFIELSEMANQKLRCKE--KWTFNGLIKHLFKEQLYKRKSY 191

Query: 175 TMSRWDQEWLTHNQIKYACFDAFVSYEIGRHL 205
             S WD   LT +Q  YA  DA+    I + L
Sbjct: 192 RCSNWDIFLLTEDQKLYAATDAYAGLLIYKKL 221

[Close]
BLAST of CmaCh14G005050 vs. TrEMBL
Match: E5GBP0_CUCME (3'-5' exonuclease OS=Cucumis melo subsp. melo PE=4 SV=1)

HSP 1 Score: 332.8 bits (852), Expect = 3.0e-88
Identity = 157/209 (75.12%), Postives = 182/209 (87.08%), Query Frame = 1 

Query: 1   MALTIVEREDPSGSHNYFDVTFDSDQPILTLLTTSPFMVEDWIAETLNTQTSPLVVGLDI 60
           M++TIV+ E PS SHNYFD+TFD+D+PILTLLTTSP MV+DWI++TL  QT PL+VGLDI
Sbjct: 1   MSITIVDHEVPSDSHNYFDITFDNDEPILTLLTTSPSMVDDWISQTLTIQTPPLLVGLDI 60

Query: 61  EWRPNSRSYENPVATLQLCTGSRCLIMQLIYTPEFPISLYEFLENESISFVGVGIEDDAE 120
           EWRPN+RSY+NPVATLQLC G RCLI+QL++ PE P SL+EFLENES +FVGVGI++DAE
Sbjct: 61  EWRPNNRSYDNPVATLQLCIGRRCLILQLLHIPEIPKSLFEFLENESFTFVGVGIDEDAE 120

Query: 121 KLNCDYGLKVRKTVDLRNLAERVTGRRELKNAGLKSLAKEILGKEIRKPRGVTMSRWDQE 180
           KLNCDYGLKV K +DLR+LAE VTGR ELKNAGLK L KE+LGKEI KP+ VTMSRWDQE
Sbjct: 121 KLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQE 180

Query: 181 WLTHNQIKYACFDAFVSYEIGRHLLSSSS 210
           WLT NQ+KYAC DAF S+EIGR L S SS
Sbjct: 181 WLTLNQVKYACIDAFFSFEIGRFLQSESS 209

[Close]
BLAST of CmaCh14G005050 vs. TrEMBL
Match: A0A0A0LBN3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G819870 PE=4 SV=1)

HSP 1 Score: 328.6 bits (841), Expect = 5.7e-87
Identity = 155/208 (74.52%), Postives = 181/208 (87.02%), Query Frame = 1 

Query: 1   MALTIVEREDPSGSHNYFDVTFDSDQPILTLLTTSPFMVEDWIAETLNTQTSPLVVGLDI 60
           MA+TIV+ E PS SHNYFD+TFD+D+PILTLLTTSP MV+ WI++TL  QT PL+VGLDI
Sbjct: 1   MAITIVDHEVPSDSHNYFDITFDTDEPILTLLTTSPSMVDGWISQTLAIQTPPLLVGLDI 60

Query: 61  EWRPNSRSYENPVATLQLCTGSRCLIMQLIYTPEFPISLYEFLENESISFVGVGIEDDAE 120
           EWRPN+RSY+NPVATLQLC G RCLI+QL++ PE P SL+EFLENES +FVGVGI++DAE
Sbjct: 61  EWRPNNRSYDNPVATLQLCIGRRCLILQLLHMPEIPKSLFEFLENESFTFVGVGIDEDAE 120

Query: 121 KLNCDYGLKVRKTVDLRNLAERVTGRRELKNAGLKSLAKEILGKEIRKPRGVTMSRWDQE 180
           KLNCDYGLKV K +DLR+LAE VTGR ELKNAGLK L KE+LGKEI KP+ VTMSRWDQE
Sbjct: 121 KLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQE 180

Query: 181 WLTHNQIKYACFDAFVSYEIGRHLLSSS 209
           WLT NQ+KYAC DAF S+EIG+ L S+S
Sbjct: 181 WLTLNQVKYACIDAFFSFEIGKFLQSAS 208

[Close]
BLAST of CmaCh14G005050 vs. TrEMBL
Match: B9SNW3_RICCO (3-5 exonuclease, putative OS=Ricinus communis GN=RCOM_0582440 PE=4 SV=1)

HSP 1 Score: 241.1 bits (614), Expect = 1.2e-60
Identity = 124/210 (59.05%), Postives = 152/210 (72.38%), Query Frame = 1 

Query: 1   MALTIVEREDPSGSHNYFDVTFDSDQPILTLLTTSPFMVEDWIAETL--NTQTSPLVVGL 60
           M L I + E  + +HN +D+TF +DQ I TL+T SP +VE W+ ET   N QT P +VGL
Sbjct: 1   MTLGIRDYELINDTHNLYDITFFTDQ-IHTLVTHSPSLVEQWLIETQGQNNQTQPTIVGL 60

Query: 61  DIEWRPN-SRSYENPVATLQLCTGSRCLIMQLIYTPEFPISLYEFLENESISFVGVGIED 120
           D+EWRPN SR  ENPVATLQLC GSRCLI QLI++P  P SL++FL+N S  F GVGIE 
Sbjct: 61  DVEWRPNFSRHIENPVATLQLCIGSRCLIYQLIHSPRIPQSLFDFLKNSSYVFAGVGIES 120

Query: 121 DAEKLNCDYGLKVRKTVDLRNLAERVTGRRELKNAGLKSLAKEILGKEIRKPRGVTMSRW 180
           D EKL  DYGL V   ++LR +A    G +ELKNAGLK L K++LGKEI+KP+ VTMSRW
Sbjct: 121 DVEKLVEDYGLSVGNVMELRRVAAESLGVKELKNAGLKELVKQVLGKEIQKPKRVTMSRW 180

Query: 181 DQEWLTHNQIKYACFDAFVSYEIGRHLLSS 208
           D  WL+H+Q++YAC DAFV  EIGR L  S
Sbjct: 181 DSMWLSHDQVQYACLDAFVCSEIGRILTQS 209

[Close]
BLAST of CmaCh14G005050 vs. TrEMBL
Match: A0A0A0LHC5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G819830 PE=4 SV=1)

HSP 1 Score: 227.3 bits (578), Expect = 1.8e-56
Identity = 118/208 (56.73%), Postives = 149/208 (71.63%), Query Frame = 1 

Query: 3   LTIVEREDPSGSHNYFDVTFDSDQPILTLLTTSPFMVEDWIAETLNT---QTSPLVVGLD 62
           LTI +R+ P  SH+Y+D+TFD D+ ILTL+T SP MV+ WI+E L     +   LVVGLD
Sbjct: 4   LTISDRQIPLNSHDYYDITFDDDE-ILTLVTASPSMVDMWISEILRIHRRRLHRLVVGLD 63

Query: 63  IEWRPN-SRSYENPVATLQLCTGSRCLIMQLIYTPEFPISLYEFLENESISFVGVGIEDD 122
           +EWRPN  R + NPVATLQLC G RCLI QLI+  E P SL +FLE+++ +FVGVGI++D
Sbjct: 64  VEWRPNFDRHFRNPVATLQLCVGRRCLIFQLIHASETPQSLIDFLEDDTFTFVGVGIDND 123

Query: 123 AEKLNCDYGLKVRKTVDLRNLAERVTGRRELKNAGLKSLAKEILGKEIRKPRGVTMSRWD 182
             KL  DY L V  TVDLR LA       E + AGLK+L +E+LG+EI KPR V +SRWD
Sbjct: 124 VLKLYNDYDLNVANTVDLRELAADEMQSDEFRTAGLKTLGREVLGREIDKPRNVKLSRWD 183

Query: 183 QEWLTHNQIKYACFDAFVSYEIGRHLLS 207
           ++WL   QI YA  DAF+S+EIGR+LLS
Sbjct: 184 RQWLNPAQILYATVDAFLSFEIGRYLLS 210

[Close]
BLAST of CmaCh14G005050 vs. TrEMBL
Match: A0A103YDM5_CYNCS (3'-5' exonuclease domain-containing protein OS=Cynara cardunculus var. scolymus GN=Ccrd_014481 PE=4 SV=1)

HSP 1 Score: 218.0 bits (554), Expect = 1.1e-53
Identity = 112/212 (52.83%), Postives = 144/212 (67.92%), Query Frame = 1 

Query: 1   MALTIVEREDPSGSHNYFDVTFDSDQPILTLLTTSPFMVEDWIAETLNTQTSPL---VVG 60
           M ++IV+ E P  +HN +DVTF  +  I T++T +P  V+ WIAE        L   +VG
Sbjct: 1   MMMSIVDHELPDYTHNLYDVTF-YENTISTIVTNTPSYVDSWIAEIERIHRRHLHRLIVG 60

Query: 61  LDIEWRPN-SRSYENPVATLQLCTGSRCLIMQLIYTPEFPISLYEFLENESISFVGVGIE 120
           LD+EWRPN SR+ +NPVA LQLC G RCL+ Q++++P  P SL  FL N S +F GVGIE
Sbjct: 61  LDVEWRPNQSRNSDNPVAILQLCVGKRCLVFQILHSPTVPESLKNFLRNPSYTFAGVGIE 120

Query: 121 DDAEKLNCDYGLKVRKTVDLRNLAERVTGRRELKNAGLKSLAKEILGKEIRKPRGVTMSR 180
            D EKL  DY L V    D+R LA    G REL+NAGLKSL   +LGKEI KP+GVTMSR
Sbjct: 121 SDVEKLTVDYNLVVANMADVRALAAVKYGVRELQNAGLKSLTMTVLGKEISKPKGVTMSR 180

Query: 181 WDQEWLTHNQIKYACFDAFVSYEIGRHLLSSS 209
           WD EWLT  Q++YA  DAF+S++IGR ++S +
Sbjct: 181 WDNEWLTPAQVQYASIDAFLSFKIGRTVISGN 211

[Close]
BLAST of CmaCh14G005050 vs. TAIR10
Match: AT3G12470.1 (AT3G12470.1 Polynucleotidyl transferase, ribonuclease H-like superfamily protein)

HSP 1 Score: 97.8 bits (242), Expect = 8.3e-21
Identity = 64/193 (33.16%), Postives = 98/193 (50.78%), Query Frame = 1 

Query: 14  SHNYFDVTFDSDQPILTLLTTSPFMVEDWIAETL----NTQTSPLVVGLDIEWRPNSRSY 73
           +H    V F  D  I+T+  T+  ++  WI        N    PLVVG+ ++WRP+S   
Sbjct: 13  THQKHFVDFFGDDLIVTVTPTAS-VIRRWIRSVRSYNRNHSVHPLVVGIGVQWRPDSSDP 72

Query: 74  ENPVATLQLCTGSRCLIMQLIYTPEFPISLYEFLENESISFVGVGIEDDAEKL-NCDYGL 133
           + P  TLQLC GSRCLI+QL Y    P  L  FL +   +FVGV    D +KL  C + +
Sbjct: 73  DPPPKTLQLCVGSRCLIIQLGYNYGLPKVLRTFLADPKTTFVGVWNGQDQKKLEKCRHRV 132

Query: 134 KVRKTVDLRNLAERVTGRRELKNAGLKSLAKEILGK-EIRKPRGVTMSRWDQEWLTHNQI 193
           ++ K +D+R       G R +     + + KE LG+  +R    + MS W    L H Q+
Sbjct: 133 EIGKLLDIRMFVRDSRGAR-MCFCSFEQIVKERLGRVGVRLDPAICMSDWGVYNLNHYQV 192

Query: 194 KYACFDAFVSYEI 201
             A  +++V +++
Sbjct: 193 LQASIESYVCFKL 203

[Close]
BLAST of CmaCh14G005050 vs. TAIR10
Match: AT4G13870.2 (AT4G13870.2 Werner syndrome-like exonuclease)

HSP 1 Score: 97.4 bits (241), Expect = 1.1e-20
Identity = 55/160 (34.38%), Postives = 89/160 (55.62%), Query Frame = 1 

Query: 43  IAETLNTQTSPLVVGLDIEWRPNSRSYENP--VATLQLCTGSRCLIMQLIYTPEFPISLY 102
           + +T   ++    VGLDIEWRP+ R    P  VAT+Q+C  S    +  I+    P SL 
Sbjct: 117 VLDTKRDESGIAFVGLDIEWRPSFRKGVLPGKVATVQICVDSNYCDVMHIFHSGIPQSLQ 176

Query: 103 EFLENESISFVGVGIEDDAEKLNCDYGLKVRKTVDLRNLAERVTGRRELKNAGLKSLAKE 162
             +E+ ++  VG+GI+ D+ KL  DYG+ ++   DL +LA +  G    K  GL SL + 
Sbjct: 177 HLIEDSTLVKVGIGIDGDSVKLFHDYGVSIKDVEDLSDLANQKIGGD--KKWGLASLTET 236

Query: 163 ILGKEIRKPRGVTMSRWDQEWLTHNQIKYACFDAFVSYEI 201
           ++ KE+ KP  + +  W+   L+  Q++YA  DA+ S+ +
Sbjct: 237 LVCKELLKPNRIRLGNWEFYPLSKQQLQYAATDAYASWHL 274

[Close]
BLAST of CmaCh14G005050 vs. TAIR10
Match: AT2G36110.1 (AT2G36110.1 Polynucleotidyl transferase, ribonuclease H-like superfamily protein)

HSP 1 Score: 95.9 bits (237), Expect = 3.1e-20
Identity = 57/151 (37.75%), Postives = 85/151 (56.29%), Query Frame = 1 

Query: 53  PLVVGLDIEWRPNSRSYENPVATLQLCTGSRCLIMQLIYTPEFPISLYEFLENESISFVG 112
           PLVVGLD++W P     + P   LQLC G+RCLI+QL +    P  L  FLE+E+I+FVG
Sbjct: 55  PLVVGLDVQWTPGGS--DPPPDILQLCVGNRCLIIQLSHCKRIPEVLRSFLEDETITFVG 114

Query: 113 VGIEDDAEKL-NCDYGLKVRKTVDLRNLAERVTGRRELKNAGLKSLAKEILG-KEIRKPR 172
           V    D  KL    + L++ + +D+R+          L N+  + + +E LG K +RK +
Sbjct: 115 VWNSQDQGKLERFRHQLEIWRLLDIRHYLP-----TRLLNSSFEKIVEECLGYKGVRKDK 174

Query: 173 GVTMSRWDQEWLTHNQIKYACFDAFVSYEIG 202
            + MS W    L+H+QI  A  D +V  ++G
Sbjct: 175 EICMSNWGARSLSHDQIVQASDDVYVCCKLG 198

[Close]
BLAST of CmaCh14G005050 vs. TAIR10
Match: AT3G12410.1 (AT3G12410.1 Polynucleotidyl transferase, ribonuclease H-like superfamily protein)

HSP 1 Score: 90.5 bits (223), Expect = 1.3e-18
Identity = 68/211 (32.23%), Postives = 109/211 (51.66%), Query Frame = 1 

Query: 14  SHNYFDVTFDSDQPILTLLTTSPFMVEDWIAETL-NTQTS--PLVVGLDIEW-------- 73
           +H  + V F  D+ I+T+ T    ++  WI   L N + S  PLVVG+ ++W        
Sbjct: 14  THLEYSVDFFGDEFIVTV-TWDSSVISRWIRNVLFNNRFSSHPLVVGVGVQWTPFSYYSD 73

Query: 74  -RPNS--------RSY-ENPVATLQLCTGSRCLIMQLIYTPEFPISLYEFLENESISFVG 133
            RPN+        R Y +NP   LQLC G+RCLI+QL Y  + P +L  FL +   +FVG
Sbjct: 74  PRPNNYYADPPPIRYYSDNPADILQLCVGNRCLIIQLGYCDQVPNNLRSFLADPETTFVG 133

Query: 134 VGIEDDAEKL-NCDYGLKVRKTVDLRNLAERVTGRRELKNAGLKSLAKEILGKE-IRKPR 193
           V    DA KL  C + L++ + +D+R       G R ++ +  + + +E +G + +    
Sbjct: 134 VWNGQDAGKLARCCHQLEIGELLDIRRYVTDSWG-RSMRRSSFEEIVEECMGYQGVMLDP 193

Query: 194 GVTMSRWDQEWLTHNQIKYACFDAFVSYEIG 202
            ++MS W    L  +QI  A  DA+V +++G
Sbjct: 194 EISMSDWTAYDLDLDQILQASLDAYVCHQLG 222

[Close]
BLAST of CmaCh14G005050 vs. TAIR10
Match: AT5G48350.1 (AT5G48350.1 Polynucleotidyl transferase, ribonuclease H-like superfamily protein)

HSP 1 Score: 68.9 bits (167), Expect = 4.1e-12
Identity = 48/167 (28.74%), Postives = 72/167 (43.11%), Query Frame = 1 

Query: 48  NTQTSPLVVGLDIEWRPNSRSYENPVATLQLCTGSRCLIMQLIYTPE---------FPIS 107
           N      ++GLD E     R   N    LQLC G  CLI+QL    E          P+ 
Sbjct: 34  NKNNRKKIIGLDTERVQKGRKL-NKTVLLQLCDGDNCLIVQLPDEDEDEGEGEDDNLPLP 93

Query: 108 LYEFLENESISFVGVGIEDDAEKLNCDYGLKVRKTVDLRNLAERVTGRRELKNAGLKSLA 167
           L+ FL     +FVG+GI     +L  ++GL  +  V++      +T         +K   
Sbjct: 94  LFNFLNLPEFTFVGIGINKTMMRLESEFGLTCKNVVEIGPATWNLTN----MTTDVKFRI 153

Query: 168 KEILGKEIRKPRGVTMSRWDQEWLTHNQIKYACFDAFVSYEIGRHLL 206
             I+  E  +P    +  W++  L  NQIK A  +A+ ++ IG  LL
Sbjct: 154 SAIVSTE--RPSNAVLEDWEKFVLNKNQIKLAASNAYFAFGIGNILL 193

[Close]
BLAST of CmaCh14G005050 vs. NCBI nr
Match: gi|659085101|ref|XP_008443244.1| (PREDICTED: Werner Syndrome-like exonuclease [Cucumis melo])

HSP 1 Score: 332.8 bits (852), Expect = 4.3e-88
Identity = 157/209 (75.12%), Postives = 182/209 (87.08%), Query Frame = 1 

Query: 1   MALTIVEREDPSGSHNYFDVTFDSDQPILTLLTTSPFMVEDWIAETLNTQTSPLVVGLDI 60
           M++TIV+ E PS SHNYFD+TFD+D+PILTLLTTSP MV+DWI++TL  QT PL+VGLDI
Sbjct: 1   MSITIVDHEVPSDSHNYFDITFDNDEPILTLLTTSPSMVDDWISQTLTIQTPPLLVGLDI 60

Query: 61  EWRPNSRSYENPVATLQLCTGSRCLIMQLIYTPEFPISLYEFLENESISFVGVGIEDDAE 120
           EWRPN+RSY+NPVATLQLC G RCLI+QL++ PE P SL+EFLENES +FVGVGI++DAE
Sbjct: 61  EWRPNNRSYDNPVATLQLCIGRRCLILQLLHIPEIPKSLFEFLENESFTFVGVGIDEDAE 120

Query: 121 KLNCDYGLKVRKTVDLRNLAERVTGRRELKNAGLKSLAKEILGKEIRKPRGVTMSRWDQE 180
           KLNCDYGLKV K +DLR+LAE VTGR ELKNAGLK L KE+LGKEI KP+ VTMSRWDQE
Sbjct: 121 KLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQE 180

Query: 181 WLTHNQIKYACFDAFVSYEIGRHLLSSSS 210
           WLT NQ+KYAC DAF S+EIGR L S SS
Sbjct: 181 WLTLNQVKYACIDAFFSFEIGRFLQSESS 209

[Close]
BLAST of CmaCh14G005050 vs. NCBI nr
Match: gi|778685161|ref|XP_011652174.1| (PREDICTED: Werner Syndrome-like exonuclease [Cucumis sativus])

HSP 1 Score: 328.6 bits (841), Expect = 8.2e-87
Identity = 155/208 (74.52%), Postives = 181/208 (87.02%), Query Frame = 1 

Query: 1   MALTIVEREDPSGSHNYFDVTFDSDQPILTLLTTSPFMVEDWIAETLNTQTSPLVVGLDI 60
           MA+TIV+ E PS SHNYFD+TFD+D+PILTLLTTSP MV+ WI++TL  QT PL+VGLDI
Sbjct: 1   MAITIVDHEVPSDSHNYFDITFDTDEPILTLLTTSPSMVDGWISQTLAIQTPPLLVGLDI 60

Query: 61  EWRPNSRSYENPVATLQLCTGSRCLIMQLIYTPEFPISLYEFLENESISFVGVGIEDDAE 120
           EWRPN+RSY+NPVATLQLC G RCLI+QL++ PE P SL+EFLENES +FVGVGI++DAE
Sbjct: 61  EWRPNNRSYDNPVATLQLCIGRRCLILQLLHMPEIPKSLFEFLENESFTFVGVGIDEDAE 120

Query: 121 KLNCDYGLKVRKTVDLRNLAERVTGRRELKNAGLKSLAKEILGKEIRKPRGVTMSRWDQE 180
           KLNCDYGLKV K +DLR+LAE VTGR ELKNAGLK L KE+LGKEI KP+ VTMSRWDQE
Sbjct: 121 KLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQE 180

Query: 181 WLTHNQIKYACFDAFVSYEIGRHLLSSS 209
           WLT NQ+KYAC DAF S+EIG+ L S+S
Sbjct: 181 WLTLNQVKYACIDAFFSFEIGKFLQSAS 208

[Close]
BLAST of CmaCh14G005050 vs. NCBI nr
Match: gi|255573515|ref|XP_002527682.1| (PREDICTED: Werner Syndrome-like exonuclease [Ricinus communis])

HSP 1 Score: 241.1 bits (614), Expect = 1.7e-60
Identity = 124/210 (59.05%), Postives = 152/210 (72.38%), Query Frame = 1 

Query: 1   MALTIVEREDPSGSHNYFDVTFDSDQPILTLLTTSPFMVEDWIAETL--NTQTSPLVVGL 60
           M L I + E  + +HN +D+TF +DQ I TL+T SP +VE W+ ET   N QT P +VGL
Sbjct: 1   MTLGIRDYELINDTHNLYDITFFTDQ-IHTLVTHSPSLVEQWLIETQGQNNQTQPTIVGL 60

Query: 61  DIEWRPN-SRSYENPVATLQLCTGSRCLIMQLIYTPEFPISLYEFLENESISFVGVGIED 120
           D+EWRPN SR  ENPVATLQLC GSRCLI QLI++P  P SL++FL+N S  F GVGIE 
Sbjct: 61  DVEWRPNFSRHIENPVATLQLCIGSRCLIYQLIHSPRIPQSLFDFLKNSSYVFAGVGIES 120

Query: 121 DAEKLNCDYGLKVRKTVDLRNLAERVTGRRELKNAGLKSLAKEILGKEIRKPRGVTMSRW 180
           D EKL  DYGL V   ++LR +A    G +ELKNAGLK L K++LGKEI+KP+ VTMSRW
Sbjct: 121 DVEKLVEDYGLSVGNVMELRRVAAESLGVKELKNAGLKELVKQVLGKEIQKPKRVTMSRW 180

Query: 181 DQEWLTHNQIKYACFDAFVSYEIGRHLLSS 208
           D  WL+H+Q++YAC DAFV  EIGR L  S
Sbjct: 181 DSMWLSHDQVQYACLDAFVCSEIGRILTQS 209

[Close]
BLAST of CmaCh14G005050 vs. NCBI nr
Match: gi|1012029302|ref|XP_015951644.1| (PREDICTED: Werner Syndrome-like exonuclease [Arachis duranensis])

HSP 1 Score: 228.4 bits (581), Expect = 1.1e-56
Identity = 113/208 (54.33%), Postives = 150/208 (72.12%), Query Frame = 1 

Query: 3   LTIVEREDPSGSHNYFDVTFDSDQPILTLLTTSPFMVEDWIAETLN-----TQTSPLVVG 62
           +++V+   P   +N +DV F +   I TLLTTSP  V+ W+++TLN     T T+ ++VG
Sbjct: 24  ISVVDHNLPYDDYNLYDVIFHT-HTIHTLLTTSPSRVDTWLSDTLNHHSSSTTTTTVLVG 83

Query: 63  LDIEWRPNS-RSYENPVATLQLCTGSRCLIMQLIYTPEFPISLYEFLENESISFVGVGIE 122
           LDIEWRPNS R+  NPVATLQLC G+RCL+ Q++++P  P SL +FL NE  +F+GVG+E
Sbjct: 84  LDIEWRPNSHRNSNNPVATLQLCIGNRCLVFQILHSPSVPQSLVDFLVNEGHTFLGVGVE 143

Query: 123 DDAEKLNCDYGLKVRKTVDLRNLAERVTGRRELKNAGLKSLAKEILGKEIRKPRGVTMSR 182
           +D EKL  DY L V+  VDLRNLAE V G  E+K AGLKSLA+ +LG EI KP+ ++ SR
Sbjct: 144 EDVEKLLEDYNLNVKNFVDLRNLAENVLGESEMKRAGLKSLAERVLGLEIEKPKRISRSR 203

Query: 183 WDQEWLTHNQIKYACFDAFVSYEIGRHL 205
           WD  WLT  Q++YAC DAFVS+E+GR L
Sbjct: 204 WDNAWLTAEQVQYACIDAFVSFEVGRML 230

[Close]
BLAST of CmaCh14G005050 vs. NCBI nr
Match: gi|449437986|ref|XP_004136771.1| (PREDICTED: Werner Syndrome-like exonuclease [Cucumis sativus])

HSP 1 Score: 227.3 bits (578), Expect = 2.6e-56
Identity = 118/208 (56.73%), Postives = 149/208 (71.63%), Query Frame = 1 

Query: 3   LTIVEREDPSGSHNYFDVTFDSDQPILTLLTTSPFMVEDWIAETLNT---QTSPLVVGLD 62
           LTI +R+ P  SH+Y+D+TFD D+ ILTL+T SP MV+ WI+E L     +   LVVGLD
Sbjct: 4   LTISDRQIPLNSHDYYDITFDDDE-ILTLVTASPSMVDMWISEILRIHRRRLHRLVVGLD 63

Query: 63  IEWRPN-SRSYENPVATLQLCTGSRCLIMQLIYTPEFPISLYEFLENESISFVGVGIEDD 122
           +EWRPN  R + NPVATLQLC G RCLI QLI+  E P SL +FLE+++ +FVGVGI++D
Sbjct: 64  VEWRPNFDRHFRNPVATLQLCVGRRCLIFQLIHASETPQSLIDFLEDDTFTFVGVGIDND 123

Query: 123 AEKLNCDYGLKVRKTVDLRNLAERVTGRRELKNAGLKSLAKEILGKEIRKPRGVTMSRWD 182
             KL  DY L V  TVDLR LA       E + AGLK+L +E+LG+EI KPR V +SRWD
Sbjct: 124 VLKLYNDYDLNVANTVDLRELAADEMQSDEFRTAGLKTLGREVLGREIDKPRNVKLSRWD 183

Query: 183 QEWLTHNQIKYACFDAFVSYEIGRHLLS 207
           ++WL   QI YA  DAF+S+EIGR+LLS
Sbjct: 184 RQWLNPAQILYATVDAFLSFEIGRYLLS 210

The following BLAST results are available for this feature:
BLAST of CmaCh14G005050 vs. Swiss-Prot
Match NameE-valueIdentityDescription
WEX_ARATH1.9e-1934.38Werner Syndrome-like exonuclease OS=Arabidopsis thaliana GN=WEX PE=1 SV=1[more]
WRN_HUMAN4.0e-1736.18Werner syndrome ATP-dependent helicase OS=Homo sapiens GN=WRN PE=1 SV=2[more]
EXD2_HUMAN9.8e-1637.18Exonuclease 3'-5' domain-containing protein 2 OS=Homo sapiens GN=EXD2 PE=1 SV=2[more]
EXD2_MOUSE2.2e-1538.46Exonuclease 3'-5' domain-containing protein 2 OS=Mus musculus GN=Exd2 PE=1 SV=2[more]
WRN_XENLA1.6e-1332.89Werner syndrome ATP-dependent helicase homolog OS=Xenopus laevis GN=wrn PE=2 SV=... [more]
BLAST of CmaCh14G005050 vs. TrEMBL
Match NameE-valueIdentityDescription
E5GBP0_CUCME3.0e-8875.123'-5' exonuclease OS=Cucumis melo subsp. melo PE=4 SV=1[more]
A0A0A0LBN3_CUCSA5.7e-8774.52Uncharacterized protein OS=Cucumis sativus GN=Csa_3G819870 PE=4 SV=1[more]
B9SNW3_RICCO1.2e-6059.053-5 exonuclease, putative OS=Ricinus communis GN=RCOM_0582440 PE=4 SV=1[more]
A0A0A0LHC5_CUCSA1.8e-5656.73Uncharacterized protein OS=Cucumis sativus GN=Csa_3G819830 PE=4 SV=1[more]
A0A103YDM5_CYNCS1.1e-5352.833'-5' exonuclease domain-containing protein OS=Cynara cardunculus var. scolymus ... [more]
BLAST of CmaCh14G005050 vs. TAIR10
Match NameE-valueIdentityDescription
AT3G12470.18.3e-2133.16 Polynucleotidyl transferase, ribonuclease H-like superfamily protein[more]
AT4G13870.21.1e-2034.38 Werner syndrome-like exonuclease[more]
AT2G36110.13.1e-2037.75 Polynucleotidyl transferase, ribonuclease H-like superfamily protein[more]
AT3G12410.11.3e-1832.23 Polynucleotidyl transferase, ribonuclease H-like superfamily protein[more]
AT5G48350.14.1e-1228.74 Polynucleotidyl transferase, ribonuclease H-like superfamily protein[more]
BLAST of CmaCh14G005050 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|659085101|ref|XP_008443244.1|4.3e-8875.12PREDICTED: Werner Syndrome-like exonuclease [Cucumis melo][more]
gi|778685161|ref|XP_011652174.1|8.2e-8774.52PREDICTED: Werner Syndrome-like exonuclease [Cucumis sativus][more]
gi|255573515|ref|XP_002527682.1|1.7e-6059.05PREDICTED: Werner Syndrome-like exonuclease [Ricinus communis][more]
gi|1012029302|ref|XP_015951644.1|1.1e-5654.33PREDICTED: Werner Syndrome-like exonuclease [Arachis duranensis][more]
gi|449437986|ref|XP_004136771.1|2.6e-5656.73PREDICTED: Werner Syndrome-like exonuclease [Cucumis sativus][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR0025623'-5'_exonuclease_dom
IPR012337RNaseH-like_sf
Vocabulary: Molecular Function
TermDefinition
GO:0003676nucleic acid binding
GO:00084083'-5' exonuclease activity
Vocabulary: Biological Process
TermDefinition
GO:0006139nucleobase-containing compound metabolic process
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0090305 nucleic acid phosphodiester bond hydrolysis
biological_process GO:0006139 nucleobase-containing compound metabolic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0008408 3'-5' exonuclease activity
molecular_function GO:0003676 nucleic acid binding
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh14G005050.1CmaCh14G005050.1mRNA


InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR0025623'-5' exonuclease domainPFAMPF01612DNA_pol_A_exo1coord: 36..204
score: 3.0
IPR0025623'-5' exonuclease domainSMARTSM0047435exoneu6coord: 36..209
score: 1.
IPR012337Ribonuclease H-like domainGENE3DG3DSA:3.30.420.10coord: 15..206
score: 5.3
IPR012337Ribonuclease H-like domainunknownSSF53098Ribonuclease H-likecoord: 47..205
score: 3.24
NoneNo IPR availablePANTHERPTHR136203-5 EXONUCLEASEcoord: 1..206
score: 2.3