CmoCh04G013250 (gene) Cucurbita moschata (Rifu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmoCh04G013250
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu))
Description	(Peroxyureidoacrylate/ureidoacrylate amidohydrolase RutB) (3.3.2.1)
Location	Cmo_Chr04 : 6756927 .. 6757568 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATAACCCCAAATCAAGGGCCGCCGCCGCCGACGCCGCCACTGCAGGCGGCGGAATCTGTCCATCATACAAGAAATACGAGATCCGCCACCGACTCCCAAATCCAAAAATCGCCGCACTCTTAGTGATCGACATGCAGAACTACTTCTCCGCCATGGCCAAACCCATCTTCTCCAATCTCCTCACCACCATCCGTCTCTGCCGTGCCGCCTCCATCCCCGTCATCTTTACCCGCCACGCCCACGCCTCTGCTTCCGACAACCCCATCCTTAGCGAGTGGTGGGACGGCGATATCATCCTCGATGGCTCCCCTGCCGCCGACCTCATCCCTAATCTCGACCGGCAGGCTACCGACGAGGTCATTCGGAAGAACACCTACAGCGCGTTCAGGAACACGCGACTAGAAGACTGCCTAAAAGAGAAGGGCGTGGAAGAAGTGATTGTAACAGGGGTTATGACGAACATCTGCTGCGAGACGACGGCGCGTGAGGCGTTCGTTAGAGGGTACAGAGTGTTCTTCTCCACCGACGCGACGGCGACGGTGGACTTGGAGCTACACGATGCAACATTGAAGAATTTGGCTTATGGGTTCGCTTACTTGGTGGATTGGCAGAGGCTCCAAGAAATTTCAGGTAATTGA

mRNA sequence

ATGGATAACCCCAAATCAAGGGCCGCCGCCGCCGACGCCGCCACTGCAGGCGGCGGAATCTGTCCATCATACAAGAAATACGAGATCCGCCACCGACTCCCAAATCCAAAAATCGCCGCACTCTTAGTGATCGACATGCAGAACTACTTCTCCGCCATGGCCAAACCCATCTTCTCCAATCTCCTCACCACCATCCGTCTCTGCCGTGCCGCCTCCATCCCCGTCATCTTTACCCGCCACGCCCACGCCTCTGCTTCCGACAACCCCATCCTTAGCGAGTGGTGGGACGGCGATATCATCCTCGATGGCTCCCCTGCCGCCGACCTCATCCCTAATCTCGACCGGCAGGCTACCGACGAGGTCATTCGGAAGAACACCTACAGCGCGTTCAGGAACACGCGACTAGAAGACTGCCTAAAAGAGAAGGGCGTGGAAGAAGTGATTGTAACAGGGGTTATGACGAACATCTGCTGCGAGACGACGGCGCGTGAGGCGTTCGTTAGAGGGTACAGAGTGTTCTTCTCCACCGACGCGACGGCGACGGTGGACTTGGAGCTACACGATGCAACATTGAAGAATTTGGCTTATGGGTTCGCTTACTTGGTGGATTGGCAGAGGCTCCAAGAAATTTCAGGTAATTGA

Coding sequence (CDS)

ATGGATAACCCCAAATCAAGGGCCGCCGCCGCCGACGCCGCCACTGCAGGCGGCGGAATCTGTCCATCATACAAGAAATACGAGATCCGCCACCGACTCCCAAATCCAAAAATCGCCGCACTCTTAGTGATCGACATGCAGAACTACTTCTCCGCCATGGCCAAACCCATCTTCTCCAATCTCCTCACCACCATCCGTCTCTGCCGTGCCGCCTCCATCCCCGTCATCTTTACCCGCCACGCCCACGCCTCTGCTTCCGACAACCCCATCCTTAGCGAGTGGTGGGACGGCGATATCATCCTCGATGGCTCCCCTGCCGCCGACCTCATCCCTAATCTCGACCGGCAGGCTACCGACGAGGTCATTCGGAAGAACACCTACAGCGCGTTCAGGAACACGCGACTAGAAGACTGCCTAAAAGAGAAGGGCGTGGAAGAAGTGATTGTAACAGGGGTTATGACGAACATCTGCTGCGAGACGACGGCGCGTGAGGCGTTCGTTAGAGGGTACAGAGTGTTCTTCTCCACCGACGCGACGGCGACGGTGGACTTGGAGCTACACGATGCAACATTGAAGAATTTGGCTTATGGGTTCGCTTACTTGGTGGATTGGCAGAGGCTCCAAGAAATTTCAGGTAATTGA

Homology

[Close]

BLAST of CmoCh04G013250 vs. Swiss-Prot
Match: NIC2_ARATH (Nicotinamidase 2 OS=Arabidopsis thaliana GN=NIC2 PE=1 SV=1)

HSP 1 Score: 256.9 bits (655), Expect = 1.9e-67
Identity = 125/188 (66.49%), Postives = 154/188 (81.91%), Query Frame = 1


Query: 23  SYKKYEIRHRLPNPKIAALLVIDMQNYFSAMAKPIFSNLLTTIRLCRAASIPVIFTRHAH 82
           S + YE R R PNPKIAALLVIDMQN+F +MA+PI  N LTTI +CR ASIPV FTRH H
Sbjct: 5   SSRTYETRKREPNPKIAALLVIDMQNHFYSMAEPILQNALTTIDICRRASIPVFFTRHNH 64

Query: 83  ASASDNPILSEWWDGDIILDGSPAADLIPNLDRQAT--DEVIRKNTYSAFRNTRLEDCLK 142
            S +D+ +L EWW+GD+ILDG+  +++IP ++RQ T  DE++ K+TYSAF NT L++ L 
Sbjct: 65  KSPTDHGMLGEWWNGDLILDGTTDSEIIPEINRQVTGPDEIVEKSTYSAFNNTHLQEKLD 124

Query: 143 EKGVEEVIVTGVMTNICCETTAREAFVRGYRVFFSTDATATVDLELHDATLKNLAYGFAY 202
           + GV+EVIV GVMTN+CCETTAREAFV+G+RVFFSTDATATV+ ELH+ATL NLAYGFAY
Sbjct: 125 KIGVKEVIVIGVMTNLCCETTAREAFVKGFRVFFSTDATATVNEELHEATLMNLAYGFAY 184

Query: 203 LVDWQRLQ 209
           LVD  RL+
Sbjct: 185 LVDCDRLR 192

[Close]

BLAST of CmoCh04G013250 vs. Swiss-Prot
Match: NIC3_ARATH (Nicotinamidase 3 OS=Arabidopsis thaliana GN=NIC3 PE=1 SV=1)

HSP 1 Score: 246.1 bits (627), Expect = 3.4e-64
Identity = 120/188 (63.83%), Postives = 153/188 (81.38%), Query Frame = 1


Query: 23  SYKKYEIRHRLPNPKIAALLVIDMQNYFSAMAKPIFSNLLTTIRLCRAASIPVIFTRHAH 82
           S +KYE R R PN KIAALLVIDMQN+FS+MAKPI +N+LTTI +CR AS+PV FTRH H
Sbjct: 5   STRKYETRKRDPNSKIAALLVIDMQNHFSSMAKPILNNVLTTIDICRRASVPVFFTRHNH 64

Query: 83  ASASDNPILSEWWDGDIILDGSPAADLIPNLDRQAT--DEVIRKNTYSAFRNTRLEDCLK 142
            S +D+ +L EW +GD+ILDG+  +++I  +  Q T  DE++ KNTYSAF  TRL++ L+
Sbjct: 65  KSPTDHGMLGEWCNGDVILDGTTDSEIIQEIQGQVTGPDEMVEKNTYSAFNKTRLQENLE 124

Query: 143 EKGVEEVIVTGVMTNICCETTAREAFVRGYRVFFSTDATATVDLELHDATLKNLAYGFAY 202
           + GV+EVIV GVMTN+CCETTAREAF++G+RVFFSTDATAT + ELH+ATL NLA+GFAY
Sbjct: 125 KIGVKEVIVIGVMTNLCCETTAREAFIKGFRVFFSTDATATFNEELHEATLMNLAFGFAY 184

Query: 203 LVDWQRLQ 209
           LVD  +L+
Sbjct: 185 LVDCDKLR 192

[Close]

BLAST of CmoCh04G013250 vs. Swiss-Prot
Match: DHBB_BACSU (Isochorismatase OS=Bacillus subtilis (strain 168) GN=dhbB PE=1 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 2.4e-17
Identity = 59/178 (33.15%), Postives = 94/178 (52.81%), Query Frame = 1


Query: 33  LPNPKIAALLVIDMQNYF----SAMAKPIF---SNLLTTIRLCRAASIPVIFTRHAHA-S 92
           +P+P  A LL+ DMQNYF    +A A P+    +N+      C    IPV++T    + +
Sbjct: 25  VPDPNRAVLLIHDMQNYFVDAFTAGASPVTELSANIRKLKNQCVQLGIPVVYTAQPGSQN 84

Query: 93  ASDNPILSEWWDGDIILDGSPAADLIPNLDRQATDEVIRKNTYSAFRNTRLEDCLKEKGV 152
             D  +L+++W G  +  G     +I  L  +  D V+ K  YSAF+ T L + ++++G 
Sbjct: 85  PDDRALLTDFW-GPGLNSGPYEEKIITELAPEDDDLVLTKWRYSAFKRTNLLEMMRKEGR 144

Query: 153 EEVIVTGVMTNICCETTAREAFVRGYRVFFSTDATATVDLELHDATLKNLAYGFAYLV 203
           +++I+TG+  +I C  TA EAF+   + FF  DA A   LE H   L+  A   A+ V
Sbjct: 145 DQLIITGIYAHIGCLVTACEAFMEDIKAFFVGDAVADFSLEKHQMALEYAAGRCAFTV 201

[Close]

BLAST of CmoCh04G013250 vs. Swiss-Prot
Match: ENTB_ECO57 (Enterobactin synthase component B OS=Escherichia coli O157:H7 GN=entB PE=3 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 1.5e-16
Identity = 57/168 (33.93%), Postives = 86/168 (51.19%), Query Frame = 1


Query: 36  PKIAALLVIDMQNYFSA-------MAKPIFSNLLTTIRLCRAASIPVIFTRHA-HASASD 95
           P+ AALL+ DMQ+YF +       M + + +N+      C+  +IPV +T      S  D
Sbjct: 28  PQRAALLIHDMQDYFVSFWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDED 87

Query: 96  NPILSEWWDGDIILDGSPAADLIPNLDRQATDEVIRKNTYSAFRNTRLEDCLKEKGVEEV 155
             +L++ W G  +        ++  L   A D V+ K  YSAF  + LE  LKE G  ++
Sbjct: 88  RALLNDMW-GPGLTRSPEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQL 147

Query: 156 IVTGVMTNICCETTAREAFVRGYRVFFSTDATATVDLELHDATLKNLA 196
           I+TGV  +I C TTA +AF+R  + F   DA A    + H  +LK +A
Sbjct: 148 IITGVYAHIGCMTTATDAFMRDIKPFMVADALADFSRDEHLMSLKYVA 194

[Close]

BLAST of CmoCh04G013250 vs. Swiss-Prot
Match: ENTB_ECOLI (Enterobactin synthase component B OS=Escherichia coli (strain K12) GN=entB PE=1 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 1.5e-16
Identity = 57/168 (33.93%), Postives = 86/168 (51.19%), Query Frame = 1


Query: 36  PKIAALLVIDMQNYFSA-------MAKPIFSNLLTTIRLCRAASIPVIFTRHA-HASASD 95
           P+ AALL+ DMQ+YF +       M + + +N+      C+  +IPV +T      S  D
Sbjct: 28  PQRAALLIHDMQDYFVSFWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDED 87

Query: 96  NPILSEWWDGDIILDGSPAADLIPNLDRQATDEVIRKNTYSAFRNTRLEDCLKEKGVEEV 155
             +L++ W G  +        ++  L   A D V+ K  YSAF  + LE  LKE G  ++
Sbjct: 88  RALLNDMW-GPGLTRSPEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQL 147

Query: 156 IVTGVMTNICCETTAREAFVRGYRVFFSTDATATVDLELHDATLKNLA 196
           I+TGV  +I C TTA +AF+R  + F   DA A    + H  +LK +A
Sbjct: 148 IITGVYAHIGCMTTATDAFMRDIKPFMVADALADFSRDEHLMSLKYVA 194

[Close]

BLAST of CmoCh04G013250 vs. TrEMBL
Match: A0A0A0KLA3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G175740 PE=4 SV=1)

HSP 1 Score: 358.6 bits (919), Expect = 5.2e-96
Identity = 179/211 (84.83%), Postives = 193/211 (91.47%), Query Frame = 1


Query: 1   MDNPKSRAAAADAATAGGGICPSYKKYEIRHRLPNPKIAALLVIDMQNYFSAMAKPIFSN 60
           MDNPKS+ +AA          PSYKKYEIRHRLPNPK+AALLVIDMQNYFSAMAKPIF +
Sbjct: 1   MDNPKSKPSAA---------IPSYKKYEIRHRLPNPKVAALLVIDMQNYFSAMAKPIFPH 60

Query: 61  LLTTIRLCRAASIPVIFTRHAHASASDNPILSEWWDGDIILDGSPAADLIPNLDRQATDE 120
           +LTTIRLCRAASIPVIFTRHAHASASDNPIL+EWW+GDIIL+G+PAA LI +LDR+  DE
Sbjct: 61  ILTTIRLCRAASIPVIFTRHAHASASDNPILNEWWNGDIILEGTPAAGLISDLDREDADE 120

Query: 121 VIRKNTYSAFRNTRLEDCLKEKGVEEVIVTGVMTNICCETTAREAFVRGYRVFFSTDATA 180
           VIRKNTYSAFRNTRLEDCLKEKGVEEVIVTGVMTNICCETTAR+AFVRGYRVFFSTDATA
Sbjct: 121 VIRKNTYSAFRNTRLEDCLKEKGVEEVIVTGVMTNICCETTARDAFVRGYRVFFSTDATA 180

Query: 181 TVDLELHDATLKNLAYGFAYLVDWQRLQEIS 212
           TVDLELH+ATLKNLAYGFAYLVD +RL EIS
Sbjct: 181 TVDLELHEATLKNLAYGFAYLVDCKRLGEIS 202

[Close]

BLAST of CmoCh04G013250 vs. TrEMBL
Match: M5XSY3_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa011697mg PE=4 SV=1)

HSP 1 Score: 283.5 bits (724), Expect = 2.1e-73
Identity = 134/186 (72.04%), Postives = 160/186 (86.02%), Query Frame = 1


Query: 23  SYKKYEIRHRLPNPKIAALLVIDMQNYFSAMAKPIFSNLLTTIRLCRAASIPVIFTRHAH 82
           SY+KY++R R PNPK+AALLVID+QNYFS++A+PI  NLLTTIRLCR ASIPVIFTRH+H
Sbjct: 5   SYEKYDVRKRNPNPKVAALLVIDVQNYFSSIAQPILQNLLTTIRLCRRASIPVIFTRHSH 64

Query: 83  ASASDNPILSEWWDGDIILDGSPAADLIPNLDRQATDEVIRKNTYSAFRNTRLEDCLKEK 142
            S SD  +L EWW GD+I DG+P + L+P L+  A D V+ KNTYSAFRNTRL++ L+E+
Sbjct: 65  KSPSDYGMLDEWWRGDLIFDGTPESHLMPELNPNAQDHVVHKNTYSAFRNTRLQEYLQER 124

Query: 143 GVEEVIVTGVMTNICCETTAREAFVRGYRVFFSTDATATVDLELHDATLKNLAYGFAYLV 202
           GVEE+IVTGVMTN+CCETTAR+AFVRG+RVFFSTDAT T D ELH+ATLKNLAYGFAYLV
Sbjct: 125 GVEELIVTGVMTNLCCETTARDAFVRGFRVFFSTDATGTSDPELHEATLKNLAYGFAYLV 184

Query: 203 DWQRLQ 209
           D  RL+
Sbjct: 185 DCNRLE 190

[Close]

BLAST of CmoCh04G013250 vs. TrEMBL
Match: A0A061E9Z8_THECC (Nicotinamidase 2 OS=Theobroma cacao GN=TCM_007722 PE=4 SV=1)

HSP 1 Score: 282.7 bits (722), Expect = 3.6e-73
Identity = 137/190 (72.11%), Postives = 160/190 (84.21%), Query Frame = 1


Query: 21  CPSYKKYEIRHRLPNPKIAALLVIDMQNYFSAMAKPIFSNLLTTIRLCRAASIPVIFTRH 80
           C SYKKYEIR R PNPK +ALLVIDMQNYFS+MAKPI +N +TTI LCR ASIPV FTRH
Sbjct: 9   CSSYKKYEIRKRDPNPKTSALLVIDMQNYFSSMAKPILNNAITTINLCRQASIPVFFTRH 68

Query: 81  AHASASDNPILSEWWDGDIILDGSPAADLIPNLDRQA-TDEVIRKNTYSAFRNTRLEDCL 140
            H S +D  +L EWWD D+I DG+  ++LIP + + A  +EV+ KNTYSAF+NTRL+D L
Sbjct: 69  CHKSPADYGMLGEWWDNDLIFDGTVDSELIPEIGQLARANEVVEKNTYSAFQNTRLQDML 128

Query: 141 KEKGVEEVIVTGVMTNICCETTAREAFVRGYRVFFSTDATATVDLELHDATLKNLAYGFA 200
            EKGVEEVIVTGVMTN+CCETTAREAFV+G+RVFFSTDATAT DLELH+ATLKN+AYGFA
Sbjct: 129 LEKGVEEVIVTGVMTNLCCETTAREAFVKGFRVFFSTDATATSDLELHEATLKNMAYGFA 188

Query: 201 YLVDWQRLQE 210
           YLVD + LQ+
Sbjct: 189 YLVDCKGLQQ 198

[Close]

BLAST of CmoCh04G013250 vs. TrEMBL
Match: W9SIY9_9ROSA (Uncharacterized protein OS=Morus notabilis GN=L484_007546 PE=4 SV=1)

HSP 1 Score: 275.8 bits (704), Expect = 4.5e-71
Identity = 133/189 (70.37%), Postives = 158/189 (83.60%), Query Frame = 1


Query: 21  CPSYKKYEIRHRLPNPKIAALLVIDMQNYFSAMAKPIFSNLLTTIRLCRAASIPVIFTRH 80
           C SYKKYEIR R P+ + AALLVID+QNYFS+MAKPI  +LL TIRLCR ASIPV FTRH
Sbjct: 8   CSSYKKYEIRRRNPDRRSAALLVIDVQNYFSSMAKPILPSLLCTIRLCRRASIPVFFTRH 67

Query: 81  AHASASDNPILSEWWDGDIILDGSPAADLIPNLDRQATDEVIRKNTYSAFRNTRLEDCLK 140
            H S +D+ +L EWW+GD+ILDG+  A+L+P+LDR+  D V+ K TYSAF+ TRLE+ L+
Sbjct: 68  CHKSPADHGMLGEWWNGDLILDGTSEAELMPDLDRRPDDVVLEKTTYSAFQGTRLEEILR 127

Query: 141 EKGVEEVIVTGVMTNICCETTAREAFVRGYRVFFSTDATATVDLELHDATLKNLAYGFAY 200
           E+ VEEVIVTGVMTN+CCETT+R AFV+G+RVFFSTDATAT + ELHDA LKNLAYGFAY
Sbjct: 128 ERRVEEVIVTGVMTNLCCETTSRGAFVKGFRVFFSTDATATANEELHDAALKNLAYGFAY 187

Query: 201 LVDWQRLQE 210
           LVD  RLQE
Sbjct: 188 LVDCDRLQE 196

[Close]

BLAST of CmoCh04G013250 vs. TrEMBL
Match: G7IBE1_MEDTR (Isochorismatase hydrolase family protein OS=Medicago truncatula GN=MTR_1g038530 PE=4 SV=1)

HSP 1 Score: 274.6 bits (701), Expect = 9.9e-71
Identity = 133/186 (71.51%), Postives = 155/186 (83.33%), Query Frame = 1


Query: 23  SYKKYEIRHRLPNPKIAALLVIDMQNYFSAMAKPIFSNLLTTIRLCRAASIPVIFTRHAH 82
           SY+KYE+R R PNP+  ALLVIDMQNYFS+MA PI +NL TTI LCR ASIPVIFTRH H
Sbjct: 12  SYEKYEVRKRNPNPQTCALLVIDMQNYFSSMASPILANLNTTITLCRRASIPVIFTRHCH 71

Query: 83  ASASDNPILSEWWDGDIILDGSPAADLIPNLDRQATDEVIRKNTYSAFRNTRLEDCLKEK 142
            S SD+ +L EWW GDII+DGS  A+L+  LDR   D V+ K+TYSAFRNT LE+ LKE 
Sbjct: 72  KSPSDHGMLEEWWFGDIIMDGSIEAELMTALDRSGEDMVVEKSTYSAFRNTGLEEKLKEM 131

Query: 143 GVEEVIVTGVMTNICCETTAREAFVRGYRVFFSTDATATVDLELHDATLKNLAYGFAYLV 202
           GV+EVIVTGVMTN+CCETTAREAF+RG+RVFFSTDATAT DL+LH+ATLKN+AYGFAY +
Sbjct: 132 GVDEVIVTGVMTNLCCETTAREAFIRGFRVFFSTDATATSDLDLHEATLKNMAYGFAYFL 191

Query: 203 DWQRLQ 209
           D  RL+
Sbjct: 192 DCHRLK 197

[Close]

BLAST of CmoCh04G013250 vs. TAIR10
Match: AT5G23230.1 (AT5G23230.1 nicotinamidase 2)

HSP 1 Score: 256.9 bits (655), Expect = 1.1e-68
Identity = 125/188 (66.49%), Postives = 154/188 (81.91%), Query Frame = 1


Query: 23  SYKKYEIRHRLPNPKIAALLVIDMQNYFSAMAKPIFSNLLTTIRLCRAASIPVIFTRHAH 82
           S + YE R R PNPKIAALLVIDMQN+F +MA+PI  N LTTI +CR ASIPV FTRH H
Sbjct: 5   SSRTYETRKREPNPKIAALLVIDMQNHFYSMAEPILQNALTTIDICRRASIPVFFTRHNH 64

Query: 83  ASASDNPILSEWWDGDIILDGSPAADLIPNLDRQAT--DEVIRKNTYSAFRNTRLEDCLK 142
            S +D+ +L EWW+GD+ILDG+  +++IP ++RQ T  DE++ K+TYSAF NT L++ L 
Sbjct: 65  KSPTDHGMLGEWWNGDLILDGTTDSEIIPEINRQVTGPDEIVEKSTYSAFNNTHLQEKLD 124

Query: 143 EKGVEEVIVTGVMTNICCETTAREAFVRGYRVFFSTDATATVDLELHDATLKNLAYGFAY 202
           + GV+EVIV GVMTN+CCETTAREAFV+G+RVFFSTDATATV+ ELH+ATL NLAYGFAY
Sbjct: 125 KIGVKEVIVIGVMTNLCCETTAREAFVKGFRVFFSTDATATVNEELHEATLMNLAYGFAY 184

Query: 203 LVDWQRLQ 209
           LVD  RL+
Sbjct: 185 LVDCDRLR 192

[Close]

BLAST of CmoCh04G013250 vs. TAIR10
Match: AT5G23220.1 (AT5G23220.1 nicotinamidase 3)

HSP 1 Score: 246.1 bits (627), Expect = 1.9e-65
Identity = 120/188 (63.83%), Postives = 153/188 (81.38%), Query Frame = 1


Query: 23  SYKKYEIRHRLPNPKIAALLVIDMQNYFSAMAKPIFSNLLTTIRLCRAASIPVIFTRHAH 82
           S +KYE R R PN KIAALLVIDMQN+FS+MAKPI +N+LTTI +CR AS+PV FTRH H
Sbjct: 5   STRKYETRKRDPNSKIAALLVIDMQNHFSSMAKPILNNVLTTIDICRRASVPVFFTRHNH 64

Query: 83  ASASDNPILSEWWDGDIILDGSPAADLIPNLDRQAT--DEVIRKNTYSAFRNTRLEDCLK 142
            S +D+ +L EW +GD+ILDG+  +++I  +  Q T  DE++ KNTYSAF  TRL++ L+
Sbjct: 65  KSPTDHGMLGEWCNGDVILDGTTDSEIIQEIQGQVTGPDEMVEKNTYSAFNKTRLQENLE 124

Query: 143 EKGVEEVIVTGVMTNICCETTAREAFVRGYRVFFSTDATATVDLELHDATLKNLAYGFAY 202
           + GV+EVIV GVMTN+CCETTAREAF++G+RVFFSTDATAT + ELH+ATL NLA+GFAY
Sbjct: 125 KIGVKEVIVIGVMTNLCCETTAREAFIKGFRVFFSTDATATFNEELHEATLMNLAFGFAY 184

Query: 203 LVDWQRLQ 209
           LVD  +L+
Sbjct: 185 LVDCDKLR 192

[Close]

BLAST of CmoCh04G013250 vs. TAIR10
Match: AT3G16190.1 (AT3G16190.1 Isochorismatase family protein)

HSP 1 Score: 63.9 bits (154), Expect = 1.3e-10
Identity = 58/193 (30.05%), Postives = 81/193 (41.97%), Query Frame = 1


Query: 40  ALLVIDMQNYFSAMA--------KPIFSNLLTTIRLCRAASIPVI--------------- 99
           ALLVIDMQN F            K I  N++  + L R   I VI               
Sbjct: 9   ALLVIDMQNDFIEEGAVTQVKGGKSIVPNVIRVVELARQRGILVIWVVREHDRQGRDVEL 68

Query: 100 FTRHAHASASDNPILSEWWDGDIILDGSPAADLIPNLDRQATDEV-IRKNTYSAFRNTRL 159
           F RH ++S    P++           G+  A+L+  L     D+  I K  +SAF +T L
Sbjct: 69  FRRHNYSSEKVGPVIK----------GTVGAELVDGLMINEEDDYKIVKTRFSAFFSTNL 128

Query: 160 EDCLKEKGVEEVIVTGVMTNICCETTAREAFVRGY-RVFFSTDATATVDLELHDAT---L 205
              L+  GV ++++ GV T  C   T  +A    Y  V   TDATA    E+H A    +
Sbjct: 129 HSFLQTSGVTKLVIAGVQTPNCIRQTVFDAVALDYPNVTVITDATAAATPEIHTANILDM 188

[Close]

BLAST of CmoCh04G013250 vs. NCBI nr
Match: gi|659090178|ref|XP_008445877.1| (PREDICTED: uncharacterized protein LOC103488764 [Cucumis melo])

HSP 1 Score: 362.8 bits (930), Expect = 4.0e-97
Identity = 183/213 (85.92%), Postives = 196/213 (92.02%), Query Frame = 1


Query: 1   MDNPKSRAAAADAATAGGGICPSYKKYEIRHRLPNPKIAALLVIDMQNYFSAMAKPIFSN 60
           M+NPKS+ AAA +  A   I  SYKKYEIRHRLPNPKIAALLVIDMQNYFSAMA+PIF N
Sbjct: 1   MENPKSKPAAATSTDA---ISSSYKKYEIRHRLPNPKIAALLVIDMQNYFSAMAEPIFPN 60

Query: 61  LLTTIRLCRAASIPVIFTRHAHASASDNPILSEWWDGDIILDGSPAADLIPNLDRQATDE 120
           +LTTIRLCRAASIPVIFTRHAHASASDNPIL+EWW+GDIILDG+ AA LI +LDR+  DE
Sbjct: 61  ILTTIRLCRAASIPVIFTRHAHASASDNPILNEWWNGDIILDGTHAAGLISDLDREDADE 120

Query: 121 VIRKNTYSAFRNTRLEDCLKEKGVEEVIVTGVMTNICCETTAREAFVRGYRVFFSTDATA 180
           VIRKNTYSAFRNTRLEDCLKEKGVEEVIVTGVMTNICCETTAREAFVRGYRVFFSTDATA
Sbjct: 121 VIRKNTYSAFRNTRLEDCLKEKGVEEVIVTGVMTNICCETTAREAFVRGYRVFFSTDATA 180

Query: 181 TVDLELHDATLKNLAYGFAYLVDWQRLQEISGN 214
           TVDLELH+ATLKNLAYGFAYLVD +RL EISG+
Sbjct: 181 TVDLELHEATLKNLAYGFAYLVDCKRLGEISGS 210

[Close]

BLAST of CmoCh04G013250 vs. NCBI nr
Match: gi|778700714|ref|XP_004143697.2| (PREDICTED: protein FAR1-RELATED SEQUENCE 5-like [Cucumis sativus])

HSP 1 Score: 358.6 bits (919), Expect = 7.5e-96
Identity = 179/211 (84.83%), Postives = 193/211 (91.47%), Query Frame = 1


Query: 1   MDNPKSRAAAADAATAGGGICPSYKKYEIRHRLPNPKIAALLVIDMQNYFSAMAKPIFSN 60
           MDNPKS+ +AA          PSYKKYEIRHRLPNPK+AALLVIDMQNYFSAMAKPIF +
Sbjct: 1   MDNPKSKPSAA---------IPSYKKYEIRHRLPNPKVAALLVIDMQNYFSAMAKPIFPH 60

Query: 61  LLTTIRLCRAASIPVIFTRHAHASASDNPILSEWWDGDIILDGSPAADLIPNLDRQATDE 120
           +LTTIRLCRAASIPVIFTRHAHASASDNPIL+EWW+GDIIL+G+PAA LI +LDR+  DE
Sbjct: 61  ILTTIRLCRAASIPVIFTRHAHASASDNPILNEWWNGDIILEGTPAAGLISDLDREDADE 120

Query: 121 VIRKNTYSAFRNTRLEDCLKEKGVEEVIVTGVMTNICCETTAREAFVRGYRVFFSTDATA 180
           VIRKNTYSAFRNTRLEDCLKEKGVEEVIVTGVMTNICCETTAR+AFVRGYRVFFSTDATA
Sbjct: 121 VIRKNTYSAFRNTRLEDCLKEKGVEEVIVTGVMTNICCETTARDAFVRGYRVFFSTDATA 180

Query: 181 TVDLELHDATLKNLAYGFAYLVDWQRLQEIS 212
           TVDLELH+ATLKNLAYGFAYLVD +RL EIS
Sbjct: 181 TVDLELHEATLKNLAYGFAYLVDCKRLGEIS 202

[Close]

BLAST of CmoCh04G013250 vs. NCBI nr
Match: gi|658052341|ref|XP_008361908.1| (PREDICTED: uncharacterized protein LOC103425595 [Malus domestica])

HSP 1 Score: 294.7 bits (753), Expect = 1.3e-76
Identity = 145/202 (71.78%), Postives = 169/202 (83.66%), Query Frame = 1


Query: 10  AADAATAGGGICPSYKKYEIRHRLPNPKIAALLVIDMQNYFSAMAKPIFSNLLTTIRLCR 69
           A+ ++++      SY+KYEIR R PNPK AALLVID+QNYFSAMA+PI  NLLTTIRLCR
Sbjct: 2   ASASSSSSSSSSSSYRKYEIRKRNPNPKAAALLVIDVQNYFSAMARPILQNLLTTIRLCR 61

Query: 70  AASIPVIFTRHAHASASDNPILSEWWDGDIILDGSPAADLIPNLDRQATDE--VIRKNTY 129
            ASIPVIFTRHAH S SD  +L EWW+GD++ DG+P + L+P LDR+  DE  V  KNTY
Sbjct: 62  RASIPVIFTRHAHKSPSDYGMLDEWWNGDLVYDGTPESQLMPELDRRGPDEDRVFDKNTY 121

Query: 130 SAFRNTRLEDCLKEKGVEEVIVTGVMTNICCETTAREAFVRGYRVFFSTDATATVDLELH 189
           SAFRNT LE+ LKEKGVEEVIVTGVMTN+CCETTAREAF+RGYRVFFSTDATAT D ELH
Sbjct: 122 SAFRNTGLEEYLKEKGVEEVIVTGVMTNLCCETTAREAFIRGYRVFFSTDATATSDRELH 181

Query: 190 DATLKNLAYGFAYLVDWQRLQE 210
           +ATLKN+AYGFAYLVD +RL++
Sbjct: 182 EATLKNMAYGFAYLVDCKRLED 203

[Close]

BLAST of CmoCh04G013250 vs. NCBI nr
Match: gi|694321138|ref|XP_009351735.1| (PREDICTED: uncharacterized protein LOC103943221 [Pyrus x bretschneideri])

HSP 1 Score: 293.5 bits (750), Expect = 3.0e-76
Identity = 144/189 (76.19%), Postives = 162/189 (85.71%), Query Frame = 1


Query: 23  SYKKYEIRHRLPNPKIAALLVIDMQNYFSAMAKPIFSNLLTTIRLCRAASIPVIFTRHAH 82
           SY KYEIR R PNPK AALLVID+QNYFSAMA+PI  NLLTTIRLCR ASIPVIFTRHAH
Sbjct: 10  SYTKYEIRKRNPNPKAAALLVIDVQNYFSAMARPILQNLLTTIRLCRRASIPVIFTRHAH 69

Query: 83  ASASDNPILSEWWDGDIILDGSPAADLIPNLDRQATDE--VIRKNTYSAFRNTRLEDCLK 142
            S SD  +L EWW+GD++ DG+P + L+P LDR+  DE  V  KNTYSAFRNT LE+ LK
Sbjct: 70  KSPSDYGMLDEWWNGDLVYDGTPESQLMPELDRRGPDEDRVFDKNTYSAFRNTGLEEYLK 129

Query: 143 EKGVEEVIVTGVMTNICCETTAREAFVRGYRVFFSTDATATVDLELHDATLKNLAYGFAY 202
           EKGVEEVIVTGVMTN+CCETTAREAF+RGYRVFFSTDATAT D ELH+ATLKN+AYGFAY
Sbjct: 130 EKGVEEVIVTGVMTNLCCETTAREAFIRGYRVFFSTDATATSDRELHEATLKNMAYGFAY 189

Query: 203 LVDWQRLQE 210
           LVD +RL++
Sbjct: 190 LVDCKRLED 198

[Close]

BLAST of CmoCh04G013250 vs. NCBI nr
Match: gi|645228590|ref|XP_008221068.1| (PREDICTED: uncharacterized protein LOC103321087 [Prunus mume])

HSP 1 Score: 286.6 bits (732), Expect = 3.6e-74
Identity = 137/186 (73.66%), Postives = 160/186 (86.02%), Query Frame = 1


Query: 23  SYKKYEIRHRLPNPKIAALLVIDMQNYFSAMAKPIFSNLLTTIRLCRAASIPVIFTRHAH 82
           SY+KY+IR R PNPK+AALLVIDMQNYFS++A+PI  NLLTTIRLCR ASIPVIFTRH+H
Sbjct: 6   SYEKYDIRKRNPNPKVAALLVIDMQNYFSSIAQPILQNLLTTIRLCRRASIPVIFTRHSH 65

Query: 83  ASASDNPILSEWWDGDIILDGSPAADLIPNLDRQATDEVIRKNTYSAFRNTRLEDCLKEK 142
            S SD  +L EWW GD+I DG+P + L+P L+  A D V+ KNTYSAFRNTRL++ L+E+
Sbjct: 66  KSPSDYGMLDEWWRGDLIFDGTPESHLMPELNPDAQDHVVHKNTYSAFRNTRLQEYLQER 125

Query: 143 GVEEVIVTGVMTNICCETTAREAFVRGYRVFFSTDATATVDLELHDATLKNLAYGFAYLV 202
           GVEE+IVTGVMTN+CCETTAREAFVRG+RVFFSTDAT T D ELH+ATLKNLAYGFAYLV
Sbjct: 126 GVEELIVTGVMTNLCCETTAREAFVRGFRVFFSTDATGTSDPELHEATLKNLAYGFAYLV 185

Query: 203 DWQRLQ 209
           D  RL+
Sbjct: 186 DCNRLE 191

The following BLAST results are available for this feature:

BLAST of CmoCh04G013250 vs. Swiss-Prot


Match Name	E-value	Identity	Description
NIC2_ARATH	1.9e-67	66.49	Nicotinamidase 2 OS=Arabidopsis thaliana GN=NIC2 PE=1 SV=1	[more]
NIC3_ARATH	3.4e-64	63.83	Nicotinamidase 3 OS=Arabidopsis thaliana GN=NIC3 PE=1 SV=1	[more]
DHBB_BACSU	2.4e-17	33.15	Isochorismatase OS=Bacillus subtilis (strain 168) GN=dhbB PE=1 SV=1	[more]
ENTB_ECO57	1.5e-16	33.93	Enterobactin synthase component B OS=Escherichia coli O157:H7 GN=entB PE=3 SV=1	[more]
ENTB_ECOLI	1.5e-16	33.93	Enterobactin synthase component B OS=Escherichia coli (strain K12) GN=entB PE=1 ...	[more]

BLAST of CmoCh04G013250 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KLA3_CUCSA	5.2e-96	84.83	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G175740 PE=4 SV=1	[more]
M5XSY3_PRUPE	2.1e-73	72.04	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa011697mg PE=4 SV=1	[more]
A0A061E9Z8_THECC	3.6e-73	72.11	Nicotinamidase 2 OS=Theobroma cacao GN=TCM_007722 PE=4 SV=1	[more]
W9SIY9_9ROSA	4.5e-71	70.37	Uncharacterized protein OS=Morus notabilis GN=L484_007546 PE=4 SV=1	[more]
G7IBE1_MEDTR	9.9e-71	71.51	Isochorismatase hydrolase family protein OS=Medicago truncatula GN=MTR_1g038530 ...	[more]

BLAST of CmoCh04G013250 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G23230.1	1.1e-68	66.49	nicotinamidase 2	[more]
AT5G23220.1	1.9e-65	63.83	nicotinamidase 3	[more]
AT3G16190.1	1.3e-10	30.05	Isochorismatase family protein	[more]

BLAST of CmoCh04G013250 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659090178\|ref\|XP_008445877.1\|	4.0e-97	85.92	PREDICTED: uncharacterized protein LOC103488764 [Cucumis melo]	[more]
gi\|778700714\|ref\|XP_004143697.2\|	7.5e-96	84.83	PREDICTED: protein FAR1-RELATED SEQUENCE 5-like [Cucumis sativus]	[more]
gi\|658052341\|ref\|XP_008361908.1\|	1.3e-76	71.78	PREDICTED: uncharacterized protein LOC103425595 [Malus domestica]	[more]
gi\|694321138\|ref\|XP_009351735.1\|	3.0e-76	76.19	PREDICTED: uncharacterized protein LOC103943221 [Pyrus x bretschneideri]	[more]
gi\|645228590\|ref\|XP_008221068.1\|	3.6e-74	73.66	PREDICTED: uncharacterized protein LOC103321087 [Prunus mume]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR000868	Isochorismatase-like

Vocabulary: **Molecular Function**
Term	Definition
GO:0003824	catalytic activity

Vocabulary: **Biological Process**
Term	Definition
GO:0008152	metabolic process

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006355	regulation of transcription, DNA-templated
biological_process	GO:0019290	siderophore biosynthetic process
biological_process	GO:0008152	metabolic process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0008908	isochorismatase activity
molecular_function	GO:0008270	zinc ion binding
molecular_function	GO:0003824	catalytic activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh04G013250.1	CmoCh04G013250.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata
Date Performed: 2017-05-19


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000868	Isochorismatase-like	GENE3D	G3DSA:3.40.50.850		coord: 32..207 score: 9.3
IPR000868	Isochorismatase-like	PFAM	PF00857	Isochorismatase	coord: 40..205 score: 2.0
IPR000868	Isochorismatase-like	unknown	SSF52499	Isochorismatase-like hydrolases	coord: 28..207 score: 1.96
None	No IPR available	PANTHER	PTHR11080	PYRAZINAMIDASE/NICOTINAMIDASE	coord: 14..210 score: 3.2
None	No IPR available	PANTHER	PTHR11080:SF13	NICOTINAMIDASE 3	coord: 14..210 score: 3.2

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CmoCh04G013250	Cucsa.166140	Cucumber (Gy14) v1	cgycmoB0475
CmoCh04G013250	CmaCh04G012510	Cucurbita maxima (Rimu)	cmacmoB728
CmoCh04G013250	Cla020058	Watermelon (97103) v1	cmowmB719
CmoCh04G013250	Csa5G175740	Cucumber (Chinese Long) v2	cmocuB736
CmoCh04G013250	MELO3C011747	Melon (DHL92) v3.5.1	cmomeB644
CmoCh04G013250	ClCG02G014040	Watermelon (Charleston Gray)	cmowcgB643
CmoCh04G013250	CSPI05G08660	Wild cucumber (PI 183967)	cmocpiB746
CmoCh04G013250	Lsi04G007970	Bottle gourd (USVL1VR-Ls)	cmolsiB669
CmoCh04G013250	Cp4.1LG01g10150	Cucurbita pepo (Zucchini)	cmocpeB673
CmoCh04G013250	MELO3C011747.2	Melon (DHL92) v3.6.1	cmomedB734
CmoCh04G013250	CsaV3_5G006120	Cucumber (Chinese Long) v3	cmocucB0872
CmoCh04G013250	Cla97C02G039780	Watermelon (97103) v2	cmowmbB713
CmoCh04G013250	Bhi07G000929	Wax gourd	cmowgoB0905
CmoCh04G013250	CsGy5G006050	Cucumber (Gy14) v2	cgybcmoB651
CmoCh04G013250	Carg02056	Silver-seed gourd	carcmoB1138

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CmoCh04G013250	Cucurbita pepo (Zucchini)	cmocpeB675
CmoCh04G013250	Melon (DHL92) v3.6.1	cmomedB711
CmoCh04G013250	Silver-seed gourd	carcmoB1149
CmoCh04G013250	Cucumber (Chinese Long) v3	cmocucB0840
CmoCh04G013250	Wax gourd	cmowgoB0891
CmoCh04G013250	Cucurbita moschata (Rifu)	cmocmoB464
CmoCh04G013250	Cucurbita moschata (Rifu)	cmocmoB470
CmoCh04G013250	Cucurbita maxima (Rimu)	cmacmoB730
CmoCh04G013250	Cucurbita maxima (Rimu)	cmacmoB744
CmoCh04G013250	Melon (DHL92) v3.5.1	cmomeB623