Carg02056 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg02056
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	Isochorismatase hydrolase family protein
Location	Cucurbita_argyrosperma_scaffold_003 : 1956142 .. 1956783 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATAACCCCAAATCAAGGGCCGCCGCCGCCGACGCCGCCACTGCAGGCGGCGGAATCTGTCCATCATACAAGAAATACGAGATCCGCCACCGACTCCCAAATCCAAAAATCGCCGCACTCTTAGTGATCGACATGCAGAACTACTTCTCCGCCATGGCCAAACCCATCTTCTCCAATCTCCTCACCACCATCCGTCTCTGCCGTGCCGCCTCCATCCCCGTCATCTTTACCCGCCACGCCCACGCCTCTGCTTCCGACAACCCCATCCTTAGCGAGTGGTGGGACGGCGATATCATCCTCGATGGCTCCCCTGCCGCCGACCTCATCCCTAATCTCGACCGGCAGGCTACCGACGAGGTCATTCGGAAGAACACCTACAGCGCGTTCAGGAACACGCGACTAGAAGACTGCCTAAAAGAGAAGGGCGTGGAAGAAGTGATTGTAACAGGGGTTATGACGAACATCTGCTGCGAGACGACGGCGCGTGAGGCGTTCGTTAGAGGGTACAGAGTGTTCTTCTCCACCGACGCGACGGCGACGGTGGACTTGGAGCTACACGATGCAACATTGAAGAATTTGGCTTATGGGTTCGCTTACTTGGTGGATTGGCAGAGGCTCCAAGAAATTTCAGGTAATTGA

mRNA sequence

ATGGATAACCCCAAATCAAGGGCCGCCGCCGCCGACGCCGCCACTGCAGGCGGCGGAATCTGTCCATCATACAAGAAATACGAGATCCGCCACCGACTCCCAAATCCAAAAATCGCCGCACTCTTAGTGATCGACATGCAGAACTACTTCTCCGCCATGGCCAAACCCATCTTCTCCAATCTCCTCACCACCATCCGTCTCTGCCGTGCCGCCTCCATCCCCGTCATCTTTACCCGCCACGCCCACGCCTCTGCTTCCGACAACCCCATCCTTAGCGAGTGGTGGGACGGCGATATCATCCTCGATGGCTCCCCTGCCGCCGACCTCATCCCTAATCTCGACCGGCAGGCTACCGACGAGGTCATTCGGAAGAACACCTACAGCGCGTTCAGGAACACGCGACTAGAAGACTGCCTAAAAGAGAAGGGCGTGGAAGAAGTGATTGTAACAGGGGTTATGACGAACATCTGCTGCGAGACGACGGCGCGTGAGGCGTTCGTTAGAGGGTACAGAGTGTTCTTCTCCACCGACGCGACGGCGACGGTGGACTTGGAGCTACACGATGCAACATTGAAGAATTTGGCTTATGGGTTCGCTTACTTGGTGGATTGGCAGAGGCTCCAAGAAATTTCAGGTAATTGA

Coding sequence (CDS)

ATGGATAACCCCAAATCAAGGGCCGCCGCCGCCGACGCCGCCACTGCAGGCGGCGGAATCTGTCCATCATACAAGAAATACGAGATCCGCCACCGACTCCCAAATCCAAAAATCGCCGCACTCTTAGTGATCGACATGCAGAACTACTTCTCCGCCATGGCCAAACCCATCTTCTCCAATCTCCTCACCACCATCCGTCTCTGCCGTGCCGCCTCCATCCCCGTCATCTTTACCCGCCACGCCCACGCCTCTGCTTCCGACAACCCCATCCTTAGCGAGTGGTGGGACGGCGATATCATCCTCGATGGCTCCCCTGCCGCCGACCTCATCCCTAATCTCGACCGGCAGGCTACCGACGAGGTCATTCGGAAGAACACCTACAGCGCGTTCAGGAACACGCGACTAGAAGACTGCCTAAAAGAGAAGGGCGTGGAAGAAGTGATTGTAACAGGGGTTATGACGAACATCTGCTGCGAGACGACGGCGCGTGAGGCGTTCGTTAGAGGGTACAGAGTGTTCTTCTCCACCGACGCGACGGCGACGGTGGACTTGGAGCTACACGATGCAACATTGAAGAATTTGGCTTATGGGTTCGCTTACTTGGTGGATTGGCAGAGGCTCCAAGAAATTTCAGGTAATTGA

Protein sequence

MDNPKSRAAAADAATAGGGICPSYKKYEIRHRLPNPKIAALLVIDMQNYFSAMAKPIFSNLLTTIRLCRAASIPVIFTRHAHASASDNPILSEWWDGDIILDGSPAADLIPNLDRQATDEVIRKNTYSAFRNTRLEDCLKEKGVEEVIVTGVMTNICCETTAREAFVRGYRVFFSTDATATVDLELHDATLKNLAYGFAYLVDWQRLQEISGN

Homology

[Close]

BLAST of Carg02056 vs. NCBI nr
Match: XP_022957085.1 (nicotinamidase 2 isoform X2 [Cucurbita moschata])

HSP 1 Score: 429.5 bits (1103), Expect = 6.7e-117
Identity = 212/212 (100.00%), Postives = 212/212 (100.00%), Query Frame = 0


Query: 1   MDNPKSRAAAADAATAGGGICPSYKKYEIRHRLPNPKIAALLVIDMQNYFSAMAKPIFSN 60
           MDNPKSRAAAADAATAGGGICPSYKKYEIRHRLPNPKIAALLVIDMQNYFSAMAKPIFSN
Sbjct: 24  MDNPKSRAAAADAATAGGGICPSYKKYEIRHRLPNPKIAALLVIDMQNYFSAMAKPIFSN 83

Query: 61  LLTTIRLCRAASIPVIFTRHAHASASDNPILSEWWDGDIILDGSPAADLIPNLDRQATDE 120
           LLTTIRLCRAASIPVIFTRHAHASASDNPILSEWWDGDIILDGSPAADLIPNLDRQATDE
Sbjct: 84  LLTTIRLCRAASIPVIFTRHAHASASDNPILSEWWDGDIILDGSPAADLIPNLDRQATDE 143

Query: 121 VIRKNTYSAFRNTRLEDCLKEKGVEEVIVTGVMTNICCETTAREAFVRGYRVFFSTDATA 180
           VIRKNTYSAFRNTRLEDCLKEKGVEEVIVTGVMTNICCETTAREAFVRGYRVFFSTDATA
Sbjct: 144 VIRKNTYSAFRNTRLEDCLKEKGVEEVIVTGVMTNICCETTAREAFVRGYRVFFSTDATA 203

Query: 181 TVDLELHDATLKNLAYGFAYLVDWQRLQEISG 213
           TVDLELHDATLKNLAYGFAYLVDWQRLQEISG
Sbjct: 204 TVDLELHDATLKNLAYGFAYLVDWQRLQEISG 235

[Close]

BLAST of Carg02056 vs. NCBI nr
Match: XP_022957084.1 (nicotinamidase 2 isoform X1 [Cucurbita moschata])

HSP 1 Score: 429.5 bits (1103), Expect = 6.7e-117
Identity = 212/212 (100.00%), Postives = 212/212 (100.00%), Query Frame = 0


Query: 1   MDNPKSRAAAADAATAGGGICPSYKKYEIRHRLPNPKIAALLVIDMQNYFSAMAKPIFSN 60
           MDNPKSRAAAADAATAGGGICPSYKKYEIRHRLPNPKIAALLVIDMQNYFSAMAKPIFSN
Sbjct: 24  MDNPKSRAAAADAATAGGGICPSYKKYEIRHRLPNPKIAALLVIDMQNYFSAMAKPIFSN 83

Query: 61  LLTTIRLCRAASIPVIFTRHAHASASDNPILSEWWDGDIILDGSPAADLIPNLDRQATDE 120
           LLTTIRLCRAASIPVIFTRHAHASASDNPILSEWWDGDIILDGSPAADLIPNLDRQATDE
Sbjct: 84  LLTTIRLCRAASIPVIFTRHAHASASDNPILSEWWDGDIILDGSPAADLIPNLDRQATDE 143

Query: 121 VIRKNTYSAFRNTRLEDCLKEKGVEEVIVTGVMTNICCETTAREAFVRGYRVFFSTDATA 180
           VIRKNTYSAFRNTRLEDCLKEKGVEEVIVTGVMTNICCETTAREAFVRGYRVFFSTDATA
Sbjct: 144 VIRKNTYSAFRNTRLEDCLKEKGVEEVIVTGVMTNICCETTAREAFVRGYRVFFSTDATA 203

Query: 181 TVDLELHDATLKNLAYGFAYLVDWQRLQEISG 213
           TVDLELHDATLKNLAYGFAYLVDWQRLQEISG
Sbjct: 204 TVDLELHDATLKNLAYGFAYLVDWQRLQEISG 235

[Close]

BLAST of Carg02056 vs. NCBI nr
Match: XP_023543219.1 (nicotinamidase 2 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 416.4 bits (1069), Expect = 5.9e-113
Identity = 207/212 (97.64%), Postives = 209/212 (98.58%), Query Frame = 0


Query: 1   MDNPKSRAAAADAATAGGGICPSYKKYEIRHRLPNPKIAALLVIDMQNYFSAMAKPIFSN 60
           MDNPKSRAAAADAATAGGGICPSYKKYEIRHRLPNPKIAALLVIDMQNYFSAMAKPIFSN
Sbjct: 24  MDNPKSRAAAADAATAGGGICPSYKKYEIRHRLPNPKIAALLVIDMQNYFSAMAKPIFSN 83

Query: 61  LLTTIRLCRAASIPVIFTRHAHASASDNPILSEWWDGDIILDGSPAADLIPNLDRQATDE 120
           LLTTIRLCRAASIPVIFTRHAHASASDN ILSEWWDGDIILDG+PAADLIPNLDRQATDE
Sbjct: 84  LLTTIRLCRAASIPVIFTRHAHASASDNTILSEWWDGDIILDGTPAADLIPNLDRQATDE 143

Query: 121 VIRKNTYSAFRNTRLEDCLKEKGVEEVIVTGVMTNICCETTAREAFVRGYRVFFSTDATA 180
           VIRKNTYSAFRNTRLEDCLKEKGVEEVIVTGVMTNICCETTAREAFVRGYRVFFSTDATA
Sbjct: 144 VIRKNTYSAFRNTRLEDCLKEKGVEEVIVTGVMTNICCETTAREAFVRGYRVFFSTDATA 203

Query: 181 TVDLELHDATLKNLAYGFAYLVDWQRLQEISG 213
           TVDLELH+ATLKNLAYG AYLVD QRLQEISG
Sbjct: 204 TVDLELHEATLKNLAYGLAYLVDCQRLQEISG 235

[Close]

BLAST of Carg02056 vs. NCBI nr
Match: XP_023543224.1 (nicotinamidase 2 isoform X3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 416.4 bits (1069), Expect = 5.9e-113
Identity = 207/212 (97.64%), Postives = 209/212 (98.58%), Query Frame = 0


Query: 1   MDNPKSRAAAADAATAGGGICPSYKKYEIRHRLPNPKIAALLVIDMQNYFSAMAKPIFSN 60
           MDNPKSRAAAADAATAGGGICPSYKKYEIRHRLPNPKIAALLVIDMQNYFSAMAKPIFSN
Sbjct: 24  MDNPKSRAAAADAATAGGGICPSYKKYEIRHRLPNPKIAALLVIDMQNYFSAMAKPIFSN 83

Query: 61  LLTTIRLCRAASIPVIFTRHAHASASDNPILSEWWDGDIILDGSPAADLIPNLDRQATDE 120
           LLTTIRLCRAASIPVIFTRHAHASASDN ILSEWWDGDIILDG+PAADLIPNLDRQATDE
Sbjct: 84  LLTTIRLCRAASIPVIFTRHAHASASDNTILSEWWDGDIILDGTPAADLIPNLDRQATDE 143

Query: 121 VIRKNTYSAFRNTRLEDCLKEKGVEEVIVTGVMTNICCETTAREAFVRGYRVFFSTDATA 180
           VIRKNTYSAFRNTRLEDCLKEKGVEEVIVTGVMTNICCETTAREAFVRGYRVFFSTDATA
Sbjct: 144 VIRKNTYSAFRNTRLEDCLKEKGVEEVIVTGVMTNICCETTAREAFVRGYRVFFSTDATA 203

Query: 181 TVDLELHDATLKNLAYGFAYLVDWQRLQEISG 213
           TVDLELH+ATLKNLAYG AYLVD QRLQEISG
Sbjct: 204 TVDLELHEATLKNLAYGLAYLVDCQRLQEISG 235

[Close]

BLAST of Carg02056 vs. NCBI nr
Match: XP_023543212.1 (nicotinamidase 2 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 416.4 bits (1069), Expect = 5.9e-113
Identity = 207/212 (97.64%), Postives = 209/212 (98.58%), Query Frame = 0


Query: 1   MDNPKSRAAAADAATAGGGICPSYKKYEIRHRLPNPKIAALLVIDMQNYFSAMAKPIFSN 60
           MDNPKSRAAAADAATAGGGICPSYKKYEIRHRLPNPKIAALLVIDMQNYFSAMAKPIFSN
Sbjct: 24  MDNPKSRAAAADAATAGGGICPSYKKYEIRHRLPNPKIAALLVIDMQNYFSAMAKPIFSN 83

Query: 61  LLTTIRLCRAASIPVIFTRHAHASASDNPILSEWWDGDIILDGSPAADLIPNLDRQATDE 120
           LLTTIRLCRAASIPVIFTRHAHASASDN ILSEWWDGDIILDG+PAADLIPNLDRQATDE
Sbjct: 84  LLTTIRLCRAASIPVIFTRHAHASASDNTILSEWWDGDIILDGTPAADLIPNLDRQATDE 143

Query: 121 VIRKNTYSAFRNTRLEDCLKEKGVEEVIVTGVMTNICCETTAREAFVRGYRVFFSTDATA 180
           VIRKNTYSAFRNTRLEDCLKEKGVEEVIVTGVMTNICCETTAREAFVRGYRVFFSTDATA
Sbjct: 144 VIRKNTYSAFRNTRLEDCLKEKGVEEVIVTGVMTNICCETTAREAFVRGYRVFFSTDATA 203

Query: 181 TVDLELHDATLKNLAYGFAYLVDWQRLQEISG 213
           TVDLELH+ATLKNLAYG AYLVD QRLQEISG
Sbjct: 204 TVDLELHEATLKNLAYGLAYLVDCQRLQEISG 235

[Close]

BLAST of Carg02056 vs. TAIR10
Match: AT5G23230.1 (nicotinamidase 2)

HSP 1 Score: 256.9 bits (655), Expect = 1.1e-68
Identity = 125/188 (66.49%), Postives = 154/188 (81.91%), Query Frame = 0


Query: 23  SYKKYEIRHRLPNPKIAALLVIDMQNYFSAMAKPIFSNLLTTIRLCRAASIPVIFTRHAH 82
           S + YE R R PNPKIAALLVIDMQN+F +MA+PI  N LTTI +CR ASIPV FTRH H
Sbjct: 5   SSRTYETRKREPNPKIAALLVIDMQNHFYSMAEPILQNALTTIDICRRASIPVFFTRHNH 64

Query: 83  ASASDNPILSEWWDGDIILDGSPAADLIPNLDRQAT--DEVIRKNTYSAFRNTRLEDCLK 142
            S +D+ +L EWW+GD+ILDG+  +++IP ++RQ T  DE++ K+TYSAF NT L++ L 
Sbjct: 65  KSPTDHGMLGEWWNGDLILDGTTDSEIIPEINRQVTGPDEIVEKSTYSAFNNTHLQEKLD 124

Query: 143 EKGVEEVIVTGVMTNICCETTAREAFVRGYRVFFSTDATATVDLELHDATLKNLAYGFAY 202
           + GV+EVIV GVMTN+CCETTAREAFV+G+RVFFSTDATATV+ ELH+ATL NLAYGFAY
Sbjct: 125 KIGVKEVIVIGVMTNLCCETTAREAFVKGFRVFFSTDATATVNEELHEATLMNLAYGFAY 184

Query: 203 LVDWQRLQ 209
           LVD  RL+
Sbjct: 185 LVDCDRLR 192

[Close]

BLAST of Carg02056 vs. TAIR10
Match: AT5G23220.1 (nicotinamidase 3)

HSP 1 Score: 246.1 bits (627), Expect = 1.9e-65
Identity = 120/188 (63.83%), Postives = 153/188 (81.38%), Query Frame = 0


Query: 23  SYKKYEIRHRLPNPKIAALLVIDMQNYFSAMAKPIFSNLLTTIRLCRAASIPVIFTRHAH 82
           S +KYE R R PN KIAALLVIDMQN+FS+MAKPI +N+LTTI +CR AS+PV FTRH H
Sbjct: 5   STRKYETRKRDPNSKIAALLVIDMQNHFSSMAKPILNNVLTTIDICRRASVPVFFTRHNH 64

Query: 83  ASASDNPILSEWWDGDIILDGSPAADLIPNLDRQAT--DEVIRKNTYSAFRNTRLEDCLK 142
            S +D+ +L EW +GD+ILDG+  +++I  +  Q T  DE++ KNTYSAF  TRL++ L+
Sbjct: 65  KSPTDHGMLGEWCNGDVILDGTTDSEIIQEIQGQVTGPDEMVEKNTYSAFNKTRLQENLE 124

Query: 143 EKGVEEVIVTGVMTNICCETTAREAFVRGYRVFFSTDATATVDLELHDATLKNLAYGFAY 202
           + GV+EVIV GVMTN+CCETTAREAF++G+RVFFSTDATAT + ELH+ATL NLA+GFAY
Sbjct: 125 KIGVKEVIVIGVMTNLCCETTAREAFIKGFRVFFSTDATATFNEELHEATLMNLAFGFAY 184

Query: 203 LVDWQRLQ 209
           LVD  +L+
Sbjct: 185 LVDCDKLR 192

[Close]

BLAST of Carg02056 vs. Swiss-Prot
Match: sp|Q9FMX7|NIC2_ARATH (Nicotinamidase 2 OS=Arabidopsis thaliana OX=3702 GN=NIC2 PE=1 SV=1)

HSP 1 Score: 256.9 bits (655), Expect = 2.0e-67
Identity = 125/188 (66.49%), Postives = 154/188 (81.91%), Query Frame = 0


Query: 23  SYKKYEIRHRLPNPKIAALLVIDMQNYFSAMAKPIFSNLLTTIRLCRAASIPVIFTRHAH 82
           S + YE R R PNPKIAALLVIDMQN+F +MA+PI  N LTTI +CR ASIPV FTRH H
Sbjct: 5   SSRTYETRKREPNPKIAALLVIDMQNHFYSMAEPILQNALTTIDICRRASIPVFFTRHNH 64

Query: 83  ASASDNPILSEWWDGDIILDGSPAADLIPNLDRQAT--DEVIRKNTYSAFRNTRLEDCLK 142
            S +D+ +L EWW+GD+ILDG+  +++IP ++RQ T  DE++ K+TYSAF NT L++ L 
Sbjct: 65  KSPTDHGMLGEWWNGDLILDGTTDSEIIPEINRQVTGPDEIVEKSTYSAFNNTHLQEKLD 124

Query: 143 EKGVEEVIVTGVMTNICCETTAREAFVRGYRVFFSTDATATVDLELHDATLKNLAYGFAY 202
           + GV+EVIV GVMTN+CCETTAREAFV+G+RVFFSTDATATV+ ELH+ATL NLAYGFAY
Sbjct: 125 KIGVKEVIVIGVMTNLCCETTAREAFVKGFRVFFSTDATATVNEELHEATLMNLAYGFAY 184

Query: 203 LVDWQRLQ 209
           LVD  RL+
Sbjct: 185 LVDCDRLR 192

[Close]

BLAST of Carg02056 vs. Swiss-Prot
Match: sp|Q9FMX8|NIC3_ARATH (Nicotinamidase 3 OS=Arabidopsis thaliana OX=3702 GN=NIC3 PE=1 SV=1)

HSP 1 Score: 246.1 bits (627), Expect = 3.5e-64
Identity = 120/188 (63.83%), Postives = 153/188 (81.38%), Query Frame = 0


Query: 23  SYKKYEIRHRLPNPKIAALLVIDMQNYFSAMAKPIFSNLLTTIRLCRAASIPVIFTRHAH 82
           S +KYE R R PN KIAALLVIDMQN+FS+MAKPI +N+LTTI +CR AS+PV FTRH H
Sbjct: 5   STRKYETRKRDPNSKIAALLVIDMQNHFSSMAKPILNNVLTTIDICRRASVPVFFTRHNH 64

Query: 83  ASASDNPILSEWWDGDIILDGSPAADLIPNLDRQAT--DEVIRKNTYSAFRNTRLEDCLK 142
            S +D+ +L EW +GD+ILDG+  +++I  +  Q T  DE++ KNTYSAF  TRL++ L+
Sbjct: 65  KSPTDHGMLGEWCNGDVILDGTTDSEIIQEIQGQVTGPDEMVEKNTYSAFNKTRLQENLE 124

Query: 143 EKGVEEVIVTGVMTNICCETTAREAFVRGYRVFFSTDATATVDLELHDATLKNLAYGFAY 202
           + GV+EVIV GVMTN+CCETTAREAF++G+RVFFSTDATAT + ELH+ATL NLA+GFAY
Sbjct: 125 KIGVKEVIVIGVMTNLCCETTAREAFIKGFRVFFSTDATATFNEELHEATLMNLAFGFAY 184

Query: 203 LVDWQRLQ 209
           LVD  +L+
Sbjct: 185 LVDCDKLR 192

[Close]

BLAST of Carg02056 vs. Swiss-Prot
Match: sp|P45743|DHBB_BACSU (Isochorismatase OS=Bacillus subtilis (strain 168) OX=224308 GN=dhbB PE=1 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 2.4e-17
Identity = 59/178 (33.15%), Postives = 94/178 (52.81%), Query Frame = 0


Query: 33  LPNPKIAALLVIDMQNY----FSAMAKPI---FSNLLTTIRLCRAASIPVIFT-RHAHAS 92
           +P+P  A LL+ DMQNY    F+A A P+    +N+      C    IPV++T +    +
Sbjct: 25  VPDPNRAVLLIHDMQNYFVDAFTAGASPVTELSANIRKLKNQCVQLGIPVVYTAQPGSQN 84

Query: 93  ASDNPILSEWWDGDIILDGSPAADLIPNLDRQATDEVIRKNTYSAFRNTRLEDCLKEKGV 152
             D  +L+++W G  +  G     +I  L  +  D V+ K  YSAF+ T L + ++++G 
Sbjct: 85  PDDRALLTDFW-GPGLNSGPYEEKIITELAPEDDDLVLTKWRYSAFKRTNLLEMMRKEGR 144

Query: 153 EEVIVTGVMTNICCETTAREAFVRGYRVFFSTDATATVDLELHDATLKNLAYGFAYLV 203
           +++I+TG+  +I C  TA EAF+   + FF  DA A   LE H   L+  A   A+ V
Sbjct: 145 DQLIITGIYAHIGCLVTACEAFMEDIKAFFVGDAVADFSLEKHQMALEYAAGRCAFTV 201

[Close]

BLAST of Carg02056 vs. Swiss-Prot
Match: sp|P0ADI5|ENTB_ECO57 (Enterobactin synthase component B OS=Escherichia coli O157:H7 OX=83334 GN=entB PE=3 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 1.6e-16
Identity = 57/168 (33.93%), Postives = 87/168 (51.79%), Query Frame = 0


Query: 36  PKIAALLVIDMQNYFSA-------MAKPIFSNLLTTIRLCRAASIPVIFT-RHAHASASD 95
           P+ AALL+ DMQ+YF +       M + + +N+      C+  +IPV +T +    S  D
Sbjct: 28  PQRAALLIHDMQDYFVSFWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDED 87

Query: 96  NPILSEWWDGDIILDGSPAADLIPNLDRQATDEVIRKNTYSAFRNTRLEDCLKEKGVEEV 155
             +L++ W G  +        ++  L   A D V+ K  YSAF  + LE  LKE G  ++
Sbjct: 88  RALLNDMW-GPGLTRSPEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQL 147

Query: 156 IVTGVMTNICCETTAREAFVRGYRVFFSTDATATVDLELHDATLKNLA 196
           I+TGV  +I C TTA +AF+R  + F   DA A    + H  +LK +A
Sbjct: 148 IITGVYAHIGCMTTATDAFMRDIKPFMVADALADFSRDEHLMSLKYVA 194

[Close]

BLAST of Carg02056 vs. Swiss-Prot
Match: sp|P0ADI4|ENTB_ECOLI (Enterobactin synthase component B OS=Escherichia coli (strain K12) OX=83333 GN=entB PE=1 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 1.6e-16
Identity = 57/168 (33.93%), Postives = 87/168 (51.79%), Query Frame = 0


Query: 36  PKIAALLVIDMQNYFSA-------MAKPIFSNLLTTIRLCRAASIPVIFT-RHAHASASD 95
           P+ AALL+ DMQ+YF +       M + + +N+      C+  +IPV +T +    S  D
Sbjct: 28  PQRAALLIHDMQDYFVSFWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDED 87

Query: 96  NPILSEWWDGDIILDGSPAADLIPNLDRQATDEVIRKNTYSAFRNTRLEDCLKEKGVEEV 155
             +L++ W G  +        ++  L   A D V+ K  YSAF  + LE  LKE G  ++
Sbjct: 88  RALLNDMW-GPGLTRSPEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQL 147

Query: 156 IVTGVMTNICCETTAREAFVRGYRVFFSTDATATVDLELHDATLKNLA 196
           I+TGV  +I C TTA +AF+R  + F   DA A    + H  +LK +A
Sbjct: 148 IITGVYAHIGCMTTATDAFMRDIKPFMVADALADFSRDEHLMSLKYVA 194

[Close]

BLAST of Carg02056 vs. TrEMBL
Match: tr|A0A1S3BD81|A0A1S3BD81_CUCME (nicotinamidase 2-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103488764 PE=4 SV=1)

HSP 1 Score: 362.8 bits (930), Expect = 5.1e-97
Identity = 183/213 (85.92%), Postives = 196/213 (92.02%), Query Frame = 0


Query: 1   MDNPKSRAAAADAATAGGGICPSYKKYEIRHRLPNPKIAALLVIDMQNYFSAMAKPIFSN 60
           M+NPKS+ A   AAT+   I  SYKKYEIRHRLPNPKIAALLVIDMQNYFSAMA+PIF N
Sbjct: 1   MENPKSKPA---AATSTDAISSSYKKYEIRHRLPNPKIAALLVIDMQNYFSAMAEPIFPN 60

Query: 61  LLTTIRLCRAASIPVIFTRHAHASASDNPILSEWWDGDIILDGSPAADLIPNLDRQATDE 120
           +LTTIRLCRAASIPVIFTRHAHASASDNPIL+EWW+GDIILDG+ AA LI +LDR+  DE
Sbjct: 61  ILTTIRLCRAASIPVIFTRHAHASASDNPILNEWWNGDIILDGTHAAGLISDLDREDADE 120

Query: 121 VIRKNTYSAFRNTRLEDCLKEKGVEEVIVTGVMTNICCETTAREAFVRGYRVFFSTDATA 180
           VIRKNTYSAFRNTRLEDCLKEKGVEEVIVTGVMTNICCETTAREAFVRGYRVFFSTDATA
Sbjct: 121 VIRKNTYSAFRNTRLEDCLKEKGVEEVIVTGVMTNICCETTAREAFVRGYRVFFSTDATA 180

Query: 181 TVDLELHDATLKNLAYGFAYLVDWQRLQEISGN 214
           TVDLELH+ATLKNLAYGFAYLVD +RL EISG+
Sbjct: 181 TVDLELHEATLKNLAYGFAYLVDCKRLGEISGS 210

[Close]

BLAST of Carg02056 vs. TrEMBL
Match: tr|A0A1S4DWB8|A0A1S4DWB8_CUCME (nicotinamidase 2-like isoform X3 OS=Cucumis melo OX=3656 GN=LOC103488764 PE=4 SV=1)

HSP 1 Score: 362.5 bits (929), Expect = 6.7e-97
Identity = 183/212 (86.32%), Postives = 195/212 (91.98%), Query Frame = 0


Query: 1   MDNPKSRAAAADAATAGGGICPSYKKYEIRHRLPNPKIAALLVIDMQNYFSAMAKPIFSN 60
           M+NPKS+ A   AAT+   I  SYKKYEIRHRLPNPKIAALLVIDMQNYFSAMA+PIF N
Sbjct: 1   MENPKSKPA---AATSTDAISSSYKKYEIRHRLPNPKIAALLVIDMQNYFSAMAEPIFPN 60

Query: 61  LLTTIRLCRAASIPVIFTRHAHASASDNPILSEWWDGDIILDGSPAADLIPNLDRQATDE 120
           +LTTIRLCRAASIPVIFTRHAHASASDNPIL+EWW+GDIILDG+ AA LI +LDR+  DE
Sbjct: 61  ILTTIRLCRAASIPVIFTRHAHASASDNPILNEWWNGDIILDGTHAAGLISDLDREDADE 120

Query: 121 VIRKNTYSAFRNTRLEDCLKEKGVEEVIVTGVMTNICCETTAREAFVRGYRVFFSTDATA 180
           VIRKNTYSAFRNTRLEDCLKEKGVEEVIVTGVMTNICCETTAREAFVRGYRVFFSTDATA
Sbjct: 121 VIRKNTYSAFRNTRLEDCLKEKGVEEVIVTGVMTNICCETTAREAFVRGYRVFFSTDATA 180

Query: 181 TVDLELHDATLKNLAYGFAYLVDWQRLQEISG 213
           TVDLELH+ATLKNLAYGFAYLVD +RL EISG
Sbjct: 181 TVDLELHEATLKNLAYGFAYLVDCKRLGEISG 209

[Close]

BLAST of Carg02056 vs. TrEMBL
Match: tr|A0A1S4DX27|A0A1S4DX27_CUCME (uncharacterized protein LOC103488764 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103488764 PE=4 SV=1)

HSP 1 Score: 360.1 bits (923), Expect = 3.3e-96
Identity = 182/211 (86.26%), Postives = 194/211 (91.94%), Query Frame = 0


Query: 1   MDNPKSRAAAADAATAGGGICPSYKKYEIRHRLPNPKIAALLVIDMQNYFSAMAKPIFSN 60
           M+NPKS+ A   AAT+   I  SYKKYEIRHRLPNPKIAALLVIDMQNYFSAMA+PIF N
Sbjct: 1   MENPKSKPA---AATSTDAISSSYKKYEIRHRLPNPKIAALLVIDMQNYFSAMAEPIFPN 60

Query: 61  LLTTIRLCRAASIPVIFTRHAHASASDNPILSEWWDGDIILDGSPAADLIPNLDRQATDE 120
           +LTTIRLCRAASIPVIFTRHAHASASDNPIL+EWW+GDIILDG+ AA LI +LDR+  DE
Sbjct: 61  ILTTIRLCRAASIPVIFTRHAHASASDNPILNEWWNGDIILDGTHAAGLISDLDREDADE 120

Query: 121 VIRKNTYSAFRNTRLEDCLKEKGVEEVIVTGVMTNICCETTAREAFVRGYRVFFSTDATA 180
           VIRKNTYSAFRNTRLEDCLKEKGVEEVIVTGVMTNICCETTAREAFVRGYRVFFSTDATA
Sbjct: 121 VIRKNTYSAFRNTRLEDCLKEKGVEEVIVTGVMTNICCETTAREAFVRGYRVFFSTDATA 180

Query: 181 TVDLELHDATLKNLAYGFAYLVDWQRLQEIS 212
           TVDLELH+ATLKNLAYGFAYLVD +RL EIS
Sbjct: 181 TVDLELHEATLKNLAYGFAYLVDCKRLGEIS 208

[Close]

BLAST of Carg02056 vs. TrEMBL
Match: tr|A0A0A0KLA3|A0A0A0KLA3_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G175740 PE=4 SV=1)

HSP 1 Score: 358.6 bits (919), Expect = 9.7e-96
Identity = 179/211 (84.83%), Postives = 193/211 (91.47%), Query Frame = 0


Query: 1   MDNPKSRAAAADAATAGGGICPSYKKYEIRHRLPNPKIAALLVIDMQNYFSAMAKPIFSN 60
           MDNPKS+ +AA          PSYKKYEIRHRLPNPK+AALLVIDMQNYFSAMAKPIF +
Sbjct: 1   MDNPKSKPSAA---------IPSYKKYEIRHRLPNPKVAALLVIDMQNYFSAMAKPIFPH 60

Query: 61  LLTTIRLCRAASIPVIFTRHAHASASDNPILSEWWDGDIILDGSPAADLIPNLDRQATDE 120
           +LTTIRLCRAASIPVIFTRHAHASASDNPIL+EWW+GDIIL+G+PAA LI +LDR+  DE
Sbjct: 61  ILTTIRLCRAASIPVIFTRHAHASASDNPILNEWWNGDIILEGTPAAGLISDLDREDADE 120

Query: 121 VIRKNTYSAFRNTRLEDCLKEKGVEEVIVTGVMTNICCETTAREAFVRGYRVFFSTDATA 180
           VIRKNTYSAFRNTRLEDCLKEKGVEEVIVTGVMTNICCETTAR+AFVRGYRVFFSTDATA
Sbjct: 121 VIRKNTYSAFRNTRLEDCLKEKGVEEVIVTGVMTNICCETTARDAFVRGYRVFFSTDATA 180

Query: 181 TVDLELHDATLKNLAYGFAYLVDWQRLQEIS 212
           TVDLELH+ATLKNLAYGFAYLVD +RL EIS
Sbjct: 181 TVDLELHEATLKNLAYGFAYLVDCKRLGEIS 202

[Close]

BLAST of Carg02056 vs. TrEMBL
Match: tr|A0A2P5G0G7|A0A2P5G0G7_9ROSA (Isochorismatase-like OS=Trema orientalis OX=63057 GN=TorRG33x02_007630 PE=4 SV=1)

HSP 1 Score: 288.9 bits (738), Expect = 9.4e-75
Identity = 138/187 (73.80%), Postives = 161/187 (86.10%), Query Frame = 0


Query: 23  SYKKYEIRHRLPNPKIAALLVIDMQNYFSAMAKPIFSNLLTTIRLCRAASIPVIFTRHAH 82
           SYKKY+IR R PNP+ AALLVID+QNYFSAMAKPI  NLL TIRLCR ASIPV+FTRH H
Sbjct: 10  SYKKYDIRRRNPNPRAAALLVIDVQNYFSAMAKPILPNLLATIRLCRRASIPVLFTRHCH 69

Query: 83  ASASDNPILSEWWDGDIILDGSPAADLIPNLDRQATDEVIRKNTYSAFRNTRLEDCLKEK 142
            S +D  +L+EWW+GD+ILDG+P ADL+P+LDR+  D V+ KNTYSAF+ TRLE+ L+E 
Sbjct: 70  KSPADYGLLAEWWNGDVILDGTPDADLMPDLDRRPNDVVLDKNTYSAFQGTRLEEILREW 129

Query: 143 GVEEVIVTGVMTNICCETTAREAFVRGYRVFFSTDATATVDLELHDATLKNLAYGFAYLV 202
            VEEVIVTGVMTN+CCETTAR+AFVRG+RVFFS DATAT D ELHDA LKNLAYGFAYLV
Sbjct: 130 RVEEVIVTGVMTNLCCETTARDAFVRGFRVFFSADATATADAELHDAALKNLAYGFAYLV 189

Query: 203 DWQRLQE 210
           D ++L+E
Sbjct: 190 DCEKLEE 196

The following BLAST results are available for this feature:

BLAST of Carg02056 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022957085.1	6.7e-117	100.00	nicotinamidase 2 isoform X2 [Cucurbita moschata]	[more]
XP_022957084.1	6.7e-117	100.00	nicotinamidase 2 isoform X1 [Cucurbita moschata]	[more]
XP_023543219.1	5.9e-113	97.64	nicotinamidase 2 isoform X2 [Cucurbita pepo subsp. pepo]	[more]
XP_023543224.1	5.9e-113	97.64	nicotinamidase 2 isoform X3 [Cucurbita pepo subsp. pepo]	[more]
XP_023543212.1	5.9e-113	97.64	nicotinamidase 2 isoform X1 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Carg02056 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G23230.1	1.1e-68	66.49	nicotinamidase 2	[more]
AT5G23220.1	1.9e-65	63.83	nicotinamidase 3	[more]

BLAST of Carg02056 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q9FMX7\|NIC2_ARATH	2.0e-67	66.49	Nicotinamidase 2 OS=Arabidopsis thaliana OX=3702 GN=NIC2 PE=1 SV=1	[more]
sp\|Q9FMX8\|NIC3_ARATH	3.5e-64	63.83	Nicotinamidase 3 OS=Arabidopsis thaliana OX=3702 GN=NIC3 PE=1 SV=1	[more]
sp\|P45743\|DHBB_BACSU	2.4e-17	33.15	Isochorismatase OS=Bacillus subtilis (strain 168) OX=224308 GN=dhbB PE=1 SV=1	[more]
sp\|P0ADI5\|ENTB_ECO57	1.6e-16	33.93	Enterobactin synthase component B OS=Escherichia coli O157:H7 OX=83334 GN=entB P...	[more]
sp\|P0ADI4\|ENTB_ECOLI	1.6e-16	33.93	Enterobactin synthase component B OS=Escherichia coli (strain K12) OX=83333 GN=e...	[more]

BLAST of Carg02056 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A1S3BD81\|A0A1S3BD81_CUCME	5.1e-97	85.92	nicotinamidase 2-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103488764 PE=4 SV...	[more]
tr\|A0A1S4DWB8\|A0A1S4DWB8_CUCME	6.7e-97	86.32	nicotinamidase 2-like isoform X3 OS=Cucumis melo OX=3656 GN=LOC103488764 PE=4 SV...	[more]
tr\|A0A1S4DX27\|A0A1S4DX27_CUCME	3.3e-96	86.26	uncharacterized protein LOC103488764 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10...	[more]
tr\|A0A0A0KLA3\|A0A0A0KLA3_CUCSA	9.7e-96	84.83	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G175740 PE=4 SV=1	[more]
tr\|A0A2P5G0G7\|A0A2P5G0G7_9ROSA	9.4e-75	73.80	Isochorismatase-like OS=Trema orientalis OX=63057 GN=TorRG33x02_007630 PE=4 SV=1	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003824	catalytic activity

Vocabulary: **Biological Process**
Term	Definition
GO:0008152	metabolic process

Vocabulary: **INTERPRO**
Term	Definition
IPR000868	Isochorismatase-like
IPR036380	Isochorismatase-like_sf

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008152	metabolic process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003824	catalytic activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg02056-RA	Carg02056-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036380	Isochorismatase-like superfamily	GENE3D	G3DSA:3.40.50.850		coord: 21..207 e-value: 2.5E-55 score: 189.4
IPR036380	Isochorismatase-like superfamily	SUPERFAMILY	SSF52499	Isochorismatase-like hydrolases	coord: 28..207
IPR000868	Isochorismatase-like	PFAM	PF00857	Isochorismatase	coord: 40..205 e-value: 2.0E-44 score: 151.7
None	No IPR available	PANTHER	PTHR43540	FAMILY NOT NAMED	coord: 34..210
None	No IPR available	PANTHER	PTHR43540:SF6	NICOTINAMIDASE 2-RELATED	coord: 34..210
None	No IPR available	CDD	cd00431	cysteine_hydrolases	coord: 40..195 e-value: 3.14432E-52 score: 167.06

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Carg02056	Cla020058	Watermelon (97103) v1	carwmB0844
Carg02056	Csa5G175740	Cucumber (Chinese Long) v2	carcuB0858
Carg02056	MELO3C011747	Melon (DHL92) v3.5.1	carmeB0832
Carg02056	ClCG02G014040	Watermelon (Charleston Gray)	carwcgB0812
Carg02056	CSPI05G08660	Wild cucumber (PI 183967)	carcpiB0878
Carg02056	Cucsa.166140	Cucumber (Gy14) v1	carcgyB0963
Carg02056	CmaCh04G012510	Cucurbita maxima (Rimu)	carcmaB1170
Carg02056	CmoCh04G013250	Cucurbita moschata (Rifu)	carcmoB1138
Carg02056	Lsi04G007970	Bottle gourd (USVL1VR-Ls)	carlsiB765
Carg02056	Cp4.1LG01g10150	Cucurbita pepo (Zucchini)	carcpeB1177
Carg02056	CsGy5G006050	Cucumber (Gy14) v2	carcgybB0832
Carg02056	MELO3C011747.2	Melon (DHL92) v3.6.1	carmedB0844
Carg02056	CsaV3_5G006120	Cucumber (Chinese Long) v3	carcucB0882
Carg02056	Cla97C02G039780	Watermelon (97103) v2	carwmbB0874
Carg02056	Bhi07G000929	Wax gourd	carwgoB0981

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Carg02056	Wax gourd	carwgoB0984
Carg02056	Silver-seed gourd	carcarB625
Carg02056	Cucurbita maxima (Rimu)	carcmaB1171
Carg02056	Cucurbita moschata (Rifu)	carcmoB1139
Carg02056	Cucurbita pepo (Zucchini)	carcpeB1179