CmoCh04G010010 (gene) Cucurbita moschata (Rifu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmoCh04G010010
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu))
Description	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Location	Cmo_Chr04 : 5043355 .. 5046189 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATCTGTTGAAGGCTGAAACTGAGTTAAAACAATTGAGTATGGACATTGATCCTCCATTACAAGTTTGCTACAAGGCCCTGATGAAAGATGAAGTGGAGGGTAAAAGTGGAAGAGTGGAGGATGAGGTGGAGGAGACATATGAAGATCTACCAGTGATTGATTTAGGGCTTCTAAAGATGGGGAATTTAGAGAGGGAGAAGTGCAAAAAGGAGATAGTTGAAGCAGCGAGGAATTGGGGTTTCTTTCAAGTAATGAATCATGGGGTTCCTGAGAGGGCATTGAAGGCGTTGTTGTATGAGCAAAAGAAAGTGTTCTATCACCCTTTTGCACACAAGAGTTTGGGAAACTTTCTAAATTTGGGTGGAACTTACCGGTGGGGTAACCCTCTTGCCCTTTCTCCTTCTCAAATTTCTTGGTCTGAAGCTTTTCATATAGCTGTTTTGGATGTTTCAAGCTTGGAGGAGCATGCCACCCTCAGGTATATATGCCCTCTCCCTTGTCTGCAGTAACTCAATTCTTTCTATTTACCTTTTGTGCTTTCATGGGTTTTGTTACTTATATCATCAACTAAAAACACAATCAACCTTAATCTACCCTCCCTCACAAGGTTTAATACCTACAACTACTCAAACAAGTGAGATTAGGGTATGATCGAAACGCAAATTAACTTGGTAATCGGTAATAGCCCAACCTCACCGCTACTAGATATTGTCTTCTTTGGGTTAGGGAGAGGTTTTTCCTTTCACGTCTGTTAGGGGGAGGTTTCTACACTCTTATAAAAAAATGTTTCGTTTTCCGCCTCAACCAATGTGGGATCTCACAATCAACTCCCTTTCGAGGCCCAGCATCCTTGCTGACACTCGTTCCCTTCTCCAATCGATGTGGGACCCCCAATCCATCCCATATGGAGACCAGCGTCCTTGCTAGCACACCGCCTTGTGTCTACCCCCATTTGGGGTTCAGCCTCCTCGCTAGCACATCGCCCTGTATCTGGCTCTGATACCATTTGTAATAACCCAAGCTCACCGCAAACCGATATTGTCCTCTTTGAGCTTTCCCTTTTGGACTTCTTCTCAAGATTTTTAAAACGCGTATGCTAGAGAGAGAGGTTTACACACCCTTATAAAGAATGCTTTGTGCTTCTCCCCAACTGATGTGAGATCTGACGTAATGACTTTTTTTGAGAGACATAAAAAGTGGCATAGAGAAGGAAAGGTTGAAACTCTCAGCTGATAAGGTCATTGTACATTTTGAACATGGATTTGAGCACTAAAACTACAAGTTTTCTTTTAATTGGTATGAAGAATGAAGCATATGAGCAATCCCAATAAGTTACTACTGATGTATGTTGCTGCTTTCCCCTTGAAAACCAACAAAATGTACAAACTGTCAAGCATATTAACACAGGAGAGTGCTTTTGATTTCTGTGTCTTTTGATTGGAAGTTTTCTATTGTTTTTTTTTTTACCCTTTCACTGCCTGCCCTGGTTTTGAATTCAGTGTCTGCATTTACTTCATACAATTACCAAAGTAAAAGAAAAAAGTACTTCCATACAGCCCTCTACATGGGTTTCGCTCACATGTTTAAAAACTCTAGGATTGCAAGCTAGATCCGTGAGCCCCGCACCTCTTAATACATATTTAGGGTACCCCACAAGGACTGAAGGGCATGTGAAGTACCTGCGGGGGGGTGCTTCCACGTCAATGGAGACTGCATGGCTCCATGGGTATACCCTTAGTTTGACACCACCCCATCACATGACCATCACATGAACCAGTTTTCTAGTACCCCGTGGGGACACACCCTCCTCCATGGTCTTATCTTCATTTTTTGGTGCTGGGTTTTTAAGCAACACATTCATTTTGGCTCTTACTACTCTTCTTCTTTCATTATTACAAGTTGTTCATGAAGACCAAGCTTGAAAGAACACATTTTGGATCATACCCAGAAGTTGTTCATGAACAATTCGAGATCAATTCATGTTAATAAACTATGAACTCACTCAAACATCCTTAAAATCTTCCAAAGTTCTCAAATTTATTGGCCAAATGCCTGAATTCAAGCCCCAAAAGTGCTTTAAAGAGGGAAAAAAGTAATCTTTTCTCCTGGGTATGATCCAAAAAAAAATCAGTGCTATACATCTCTGTTTCTGTTTTTGCACAGATCCACAATGGAAACAGTTGCGAAGAAATTTGGGAGCTTAGCAGAGAGCATAGCAGAGATTTTAGGGCAAAGTTTGGGGATAAAATGGAGTTACTTCAAAGAAAGATGTGAGAAAGGAAAGAGCAGCCTTAGACTGAACAGATACCCACCTTGTCCATTTGCCTCCAAAGTGTATGGCCTTATTCCTCACACAGACAGCGATTATCTGACAATACTTTATCAAGCTCATACCAGTGGTCTGCAGCTCATGAGAGATGGAACCTGGTTTCCTGTTAAACCTAACCCACAATATTTGCTTGTCAACATCGGTGACTTACTTCAGGTGCACACTTTGTTCCCCCGACTTAAATCTTATTTCCTCCATTGATATTAATTGGTGAAAATATGATGAAAATGTGATGCAGGTTGTGAGCAATGATGTTTTCAAGAGCATAAAACACAGAGTGGTTGCTTCTGAAGAAGATGAGAGATTTTCGTTTGCATATTTCTACTGCCCATCGGAAGATGTGATGATTGAGAGCTGCATGAAACCAAGCATTTACAAGCAGTTCAGCTACAAAGAGTATAGACAACAAATAGAAAAAGACGTGGAGAAAACAGGCAACAAAGTAGGGCTTTCAAGGTTTTTCTTGCACCACATGTTGGATTTTAATTTGCTCAATGAGTAA

mRNA sequence

ATGGATCTGTTGAAGGCTGAAACTGAGTTAAAACAATTGAGTATGGACATTGATCCTCCATTACAAGTTTGCTACAAGGCCCTGATGAAAGATGAAGTGGAGGGTAAAAGTGGAAGAGTGGAGGATGAGGTGGAGGAGACATATGAAGATCTACCAGTGATTGATTTAGGGCTTCTAAAGATGGGGAATTTAGAGAGGGAGAAGTGCAAAAAGGAGATAGTTGAAGCAGCGAGGAATTGGGGTTTCTTTCAAGTAATGAATCATGGGGTTCCTGAGAGGGCATTGAAGGCGTTGTTGTATGAGCAAAAGAAAGTGTTCTATCACCCTTTTGCACACAAGAGTTTGGGAAACTTTCTAAATTTGGGTGGAACTTACCGGTGGGGTAACCCTCTTGCCCTTTCTCCTTCTCAAATTTCTTGGTCTGAAGCTTTTCATATAGCTGTTTTGGATGTTTCAAGCTTGGAGGAGCATGCCACCCTCAGATCCACAATGGAAACAGTTGCGAAGAAATTTGGGAGCTTAGCAGAGAGCATAGCAGAGATTTTAGGGCAAAGTTTGGGGATAAAATGGAGTTACTTCAAAGAAAGATGTGAGAAAGGAAAGAGCAGCCTTAGACTGAACAGATACCCACCTTGTCCATTTGCCTCCAAAGTGTATGGCCTTATTCCTCACACAGACAGCGATTATCTGACAATACTTTATCAAGCTCATACCAGTGGTCTGCAGCTCATGAGAGATGGAACCTGGTTTCCTGTTAAACCTAACCCACAATATTTGCTTGTCAACATCGGTGACTTACTTCAGGTTGTGAGCAATGATGTTTTCAAGAGCATAAAACACAGAGTGGTTGCTTCTGAAGAAGATGAGAGATTTTCGTTTGCATATTTCTACTGCCCATCGGAAGATGTGATGATTGAGAGCTGCATGAAACCAAGCATTTACAAGCAGTTCAGCTACAAAGAGTATAGACAACAAATAGAAAAAGACGTGGAGAAAACAGGCAACAAAGTAGGGCTTTCAAGGTTTTTCTTGCACCACATGTTGGATTTTAATTTGCTCAATGAGTAA

Coding sequence (CDS)

ATGGATCTGTTGAAGGCTGAAACTGAGTTAAAACAATTGAGTATGGACATTGATCCTCCATTACAAGTTTGCTACAAGGCCCTGATGAAAGATGAAGTGGAGGGTAAAAGTGGAAGAGTGGAGGATGAGGTGGAGGAGACATATGAAGATCTACCAGTGATTGATTTAGGGCTTCTAAAGATGGGGAATTTAGAGAGGGAGAAGTGCAAAAAGGAGATAGTTGAAGCAGCGAGGAATTGGGGTTTCTTTCAAGTAATGAATCATGGGGTTCCTGAGAGGGCATTGAAGGCGTTGTTGTATGAGCAAAAGAAAGTGTTCTATCACCCTTTTGCACACAAGAGTTTGGGAAACTTTCTAAATTTGGGTGGAACTTACCGGTGGGGTAACCCTCTTGCCCTTTCTCCTTCTCAAATTTCTTGGTCTGAAGCTTTTCATATAGCTGTTTTGGATGTTTCAAGCTTGGAGGAGCATGCCACCCTCAGATCCACAATGGAAACAGTTGCGAAGAAATTTGGGAGCTTAGCAGAGAGCATAGCAGAGATTTTAGGGCAAAGTTTGGGGATAAAATGGAGTTACTTCAAAGAAAGATGTGAGAAAGGAAAGAGCAGCCTTAGACTGAACAGATACCCACCTTGTCCATTTGCCTCCAAAGTGTATGGCCTTATTCCTCACACAGACAGCGATTATCTGACAATACTTTATCAAGCTCATACCAGTGGTCTGCAGCTCATGAGAGATGGAACCTGGTTTCCTGTTAAACCTAACCCACAATATTTGCTTGTCAACATCGGTGACTTACTTCAGGTTGTGAGCAATGATGTTTTCAAGAGCATAAAACACAGAGTGGTTGCTTCTGAAGAAGATGAGAGATTTTCGTTTGCATATTTCTACTGCCCATCGGAAGATGTGATGATTGAGAGCTGCATGAAACCAAGCATTTACAAGCAGTTCAGCTACAAAGAGTATAGACAACAAATAGAAAAAGACGTGGAGAAAACAGGCAACAAAGTAGGGCTTTCAAGGTTTTTCTTGCACCACATGTTGGATTTTAATTTGCTCAATGAGTAA

Homology

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BLAST of CmoCh04G010010 vs. Swiss-Prot
Match: G2OX8_ARATH (Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana GN=GA2OX8 PE=1 SV=2)

HSP 1 Score: 316.2 bits (809), Expect = 4.5e-85
Identity = 162/339 (47.79%), Postives = 217/339 (64.01%), Query Frame = 1


Query: 17  IDPPLQVCYKALMKDEVEGKSGRVED---------EVEETYEDLPVIDLGLLKMG-NLER 76
           +DPP    Y  L+ +++  K   V +          VEE   +LPVID+  L  G   ER
Sbjct: 1   MDPPFNEIYNNLLYNQITKKDNDVSEIPFSFSVTAVVEEV--ELPVIDVSRLIDGAEEER 60

Query: 77  EKCKKEIVEAARNWGFFQVMNHGVPERALKALLYEQKKVFYHPFAHKSLGNFLNLGGTYR 136
           EKCK+ I  A+R WGFFQV+NHG+    L+ +  EQ +VF  PF  KS     +  G+YR
Sbjct: 61  EKCKEAIARASREWGFFQVINHGISMDVLEKMRQEQIRVFREPFDKKSKSEKFS-AGSYR 120

Query: 137 WGNPLALSPSQISWSEAFHIAVLDVSSLEEHATLRSTMETVAKKFGSLAESIAEILGQSL 196
           WG P A S  Q+SWSEAFH+ + D+S  ++  TL STME  A +  +LA  +AE+L +  
Sbjct: 121 WGTPSATSIRQLSWSEAFHVPMTDISDNKDFTTLSSTMEKFASESEALAYMLAEVLAEKS 180

Query: 197 GIKWSYFKERCEKGKSSLRLNRYPPCPFASKVYGLIPHTDSDYLTILYQAHTSGLQLMRD 256
           G   S+FKE C +    LR+NRYPPCP  S+VYGL+PHTDSD+LTILYQ    GLQL++D
Sbjct: 181 GQNSSFFKENCVRNTCYLRMNRYPPCPKPSEVYGLMPHTDSDFLTILYQDQVGGLQLIKD 240

Query: 257 GTWFPVKPNPQYLLVNIGDLLQVVSNDVFKSIKHRVVASEEDERFSFAYFYCPSEDVMIE 316
             W  VKPNP+ L++NIGDL Q  SN ++KS++HRV+ + + ERFS AYF CPS D +IE
Sbjct: 241 NRWIAVKPNPKALIINIGDLFQAWSNGMYKSVEHRVMTNPKVERFSTAYFMCPSYDAVIE 300

Query: 317 SCMKPSIYKQFSYKEYRQQIEKDVEKTGNKVGLSRFFLH 346
                  Y+ FS++E+RQQ+++DV+K G KVGL RF  H
Sbjct: 301 CSSDRPAYRNFSFREFRQQVQEDVKKFGFKVGLPRFLNH 336

[Close]

BLAST of CmoCh04G010010 vs. Swiss-Prot
Match: G2OX7_ARATH (Gibberellin 2-beta-dioxygenase 7 OS=Arabidopsis thaliana GN=GA2OX7 PE=1 SV=1)

HSP 1 Score: 273.5 bits (698), Expect = 3.3e-72
Identity = 135/297 (45.45%), Postives = 199/297 (67.00%), Query Frame = 1


Query: 51  LPVIDLGLLKMGN-LEREKCKKEIVEAARNWGFFQVMNHGVPERALKALLYEQKKVFYHP 110
           LPVIDL  L  G  ++R++C K++V AA+ WGFFQ++NHG+P+   + +L E+KK+F  P
Sbjct: 40  LPVIDLSHLTSGEEVKRKRCVKQMVAAAKEWGFFQIVNHGIPKDVFEMMLLEEKKLFDQP 99

Query: 111 FAHKSLGNFLNLG-GTYRWGNPLALSPSQISWSEAFHIAVLDVSSL-EEHATLRSTMETV 170
           F+ K    F +L   +YRWGNP A SP+Q S SEAFHI + +VS + ++   LR+ +ET 
Sbjct: 100 FSVKVRERFSDLSKNSYRWGNPSATSPAQYSVSEAFHIILSEVSRISDDRNNLRTIVETY 159

Query: 171 AKKFGSLAESIAEILGQSLGIKWSYFKERCEKGKSSLRLNRYPPCPFASKVYGLIPHTDS 230
            ++   +A+ I EILG+ + +   YF+   E   S LRLN+Y P  F S+V+GL+PHTD+
Sbjct: 160 VQEIARVAQMICEILGKQVNVSSEYFENIFELENSFLRLNKYHPSVFGSEVFGLVPHTDT 219

Query: 231 DYLTILYQAHTSGLQLMRDGTWFPVKPNPQYLLVNIGDLLQVVSNDVFKSIKHRVVASEE 290
            +LTIL Q    GL+L  +G W  VKP  + L VNIGD+ Q +SN V++S++HRV++   
Sbjct: 220 SFLTILSQDQIGGLELENNGQWISVKPCLEALTVNIGDMFQALSNGVYQSVRHRVISPAN 279

Query: 291 DERFSFAYFYCPSEDVMIESCMKPSIYKQFSYKEYRQQIEKDVEKTGNKVGLSRFFL 345
            ER S A+F CP  +  I+    P  Y++FS++EY++Q E DV++TG+KVGLSRF +
Sbjct: 280 IERMSIAFFVCPYLETEIDCFGYPKKYRRFSFREYKEQSEHDVKETGDKVGLSRFLI 336

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BLAST of CmoCh04G010010 vs. Swiss-Prot
Match: DLO2_ARATH (Protein DMR6-LIKE OXYGENASE 2 OS=Arabidopsis thaliana GN=DLO2 PE=2 SV=1)

HSP 1 Score: 154.8 bits (390), Expect = 1.7e-36
Identity = 89/294 (30.27%), Postives = 140/294 (47.62%), Query Frame = 1


Query: 43  EVEETYEDLPVIDLGLLKMGNLEREKCKKEIVEAARNWGFFQVMNHGVPERALKALLYEQ 102
           EV+ + + +P+IDL  L   N  R     +   A  + GFFQ+ NHGVPE  +K ++   
Sbjct: 34  EVQTSGDSIPLIDLHDLHGPN--RADIINQFAHACSSCGFFQIKNHGVPEETIKKMMNAA 93

Query: 103 KKVFYHPFAHKSLGNFLNLGGTYRWGNPLALSPSQIS-WSEAFHIAVLDVSSLEEH---- 162
           ++ F    + +      +   T R      +S  ++S W +   +    +          
Sbjct: 94  REFFRQSESERVKHYSADTKKTTRLSTSFNVSKEKVSNWRDFLRLHCYPIEDFINEWPST 153

Query: 163 -ATLRSTMETVAKKFGSLAESIAEILGQSLGIKWSYFKERCEKGKSSLRLNRYPPCPFAS 222
             + R      A    +L  ++ E + +SLG+          K    + +N YP CP   
Sbjct: 154 PISFREVTAEYATSVRALVLTLLEAISESLGLAKDRVSNTIGKHGQHMAINYYPRCPQPE 213

Query: 223 KVYGLIPHTDSDYLTILYQAHTSGLQLMRDGTWFPVKPNPQYLLVNIGDLLQVVSNDVFK 282
             YGL  H D++ +T+L Q   SGLQ+ +DG W  V P P   +VN+GD +QV+SN+ +K
Sbjct: 214 LTYGLPGHKDANLITVLLQDEVSGLQVFKDGKWIAVNPVPNTFIVNLGDQMQVISNEKYK 273

Query: 283 SIKHRVVASEEDERFSFAYFYCPSEDVMIESCMK--------PSIYKQFSYKEY 323
           S+ HR V + + ER S   FYCPSED +I    +        P+IY+ F+Y EY
Sbjct: 274 SVLHRAVVNSDMERISIPTFYCPSEDAVISPAQELINEEEDSPAIYRNFTYAEY 325

[Close]

BLAST of CmoCh04G010010 vs. Swiss-Prot
Match: ACCO2_ARATH (1-aminocyclopropane-1-carboxylate oxidase 2 OS=Arabidopsis thaliana GN=ACO2 PE=2 SV=2)

HSP 1 Score: 152.5 bits (384), Expect = 8.5e-36
Identity = 90/283 (31.80%), Postives = 142/283 (50.18%), Query Frame = 1


Query: 52  PVIDLGLLKMGNLEREKCKKEIVEAARNWGFFQVMNHGVPERALKALLYEQKKVFYHPFA 111
           PV+DL   K+   ER++    I EA  NWGFF+++NHG+P   +  +  +  K  Y    
Sbjct: 8   PVVDLS--KLNGEERDQTMALINEACENWGFFEIVNHGLPHDLMDKI-EKMTKDHYKTCQ 67

Query: 112 HKSLGNFLNLGGTYRWGNPLALSPSQISWSEAFHIAVLDVSSLEEHATLRSTMETVAKKF 171
            +   + L   G     + L      + W   F++  L  S+L + + +     T  K F
Sbjct: 68  EQKFNDMLKSKGL----DNLETEVEDVDWESTFYVRHLPQSNLNDISDVSDEYRTAMKDF 127

Query: 172 G----SLAESIAEILGQSLGIKWSYFKERCEKGKS---SLRLNRYPPCPFASKVYGLIPH 231
           G    +LAE + ++L ++LG++  Y K+     K      +++ YPPCP    + GL  H
Sbjct: 128 GKRLENLAEDLLDLLCENLGLEKGYLKKVFHGTKGPTFGTKVSNYPPCPKPEMIKGLRAH 187

Query: 232 TDSDYLTILYQAH-TSGLQLMRDGTWFPVKPNPQYLLVNIGDLLQVVSNDVFKSIKHRVV 291
           TD+  + +L+Q    SGLQL++DG W  V P    +++N+GD L+V++N  +KS+ HRVV
Sbjct: 188 TDAGGIILLFQDDKVSGLQLLKDGDWIDVPPLNHSIVINLGDQLEVITNGKYKSVLHRVV 247

Query: 292 ASEEDERFSFAYFYCPSEDVMIESCM----KPSIYKQFSYKEY 323
             +E  R S A FY P  D  I        K S Y  F + +Y
Sbjct: 248 TQQEGNRMSVASFYNPGSDAEISPATSLVEKDSEYPSFVFDDY 283

[Close]

BLAST of CmoCh04G010010 vs. Swiss-Prot
Match: ACCO3_PETHY (1-aminocyclopropane-1-carboxylate oxidase 3 OS=Petunia hybrida GN=ACO3 PE=3 SV=1)

HSP 1 Score: 147.5 bits (371), Expect = 2.7e-34
Identity = 89/290 (30.69%), Postives = 148/290 (51.03%), Query Frame = 1


Query: 49  EDLPVIDLGLLKMGNLEREKCKKEIVEAARNWGFFQVMNHGVPERALKALLYEQKKVFYH 108
           E+ P+I+L   K+   ER+   + I +A  NWGFF+++NHG+P   +  +  +  K  Y 
Sbjct: 2   ENFPIINLE--KLNGSERDATMEMIKDACENWGFFELVNHGIPHEVMDTV-EKLTKGHYK 61

Query: 109 PFAHKSLGNFLNLGGTYRWGNPLALSPSQISWSEAFHIAVLDVSSLEEHATL----RSTM 168
               +     +   G       +    + + W   F +  L VS++ E   L    R  M
Sbjct: 62  KCMEQRFKELVASKGL----EAVQAEVTDLDWESTFFLRHLPVSNISEVPDLDDEYREVM 121

Query: 169 ETVAKKFGSLAESIAEILGQSLGIKWSYFKERCEKGKS---SLRLNRYPPCPFASKVYGL 228
              AK+   LAE + ++L ++LG++  Y K+     K      +++ YPPCP    + GL
Sbjct: 122 RDFAKRLEKLAEELLDLLCENLGLEKGYLKKAFYGSKGPNFGTKVSNYPPCPKPDLIKGL 181

Query: 229 IPHTDSDYLTILYQAH-TSGLQLMRDGTWFPVKPNPQYLLVNIGDLLQVVSNDVFKSIKH 288
             HTD+  + +L+Q    SGLQL++DG W  V P    ++VN+GD L+V++N  +KS+ H
Sbjct: 182 RAHTDAGGIILLFQDDKVSGLQLLKDGQWIDVPPMRHSIVVNLGDQLEVITNGKYKSVLH 241

Query: 289 RVVASEEDERFSFAYFYCPSEDVMIESCMKPSIYKQFSYKEYRQQIEKDV 331
           RV+A  +  R S A FY P  D +I     P++ ++ + +E +Q   K V
Sbjct: 242 RVIAQTDGTRMSLASFYNPGSDAVIYPA--PTLVEKEADQECKQVYPKFV 282

[Close]

BLAST of CmoCh04G010010 vs. TrEMBL
Match: K7LW87_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_12G214000 PE=3 SV=1)

HSP 1 Score: 372.5 bits (955), Expect = 5.8e-100
Identity = 188/339 (55.46%), Postives = 247/339 (72.86%), Query Frame = 1


Query: 15  MDIDPPLQVCYKALMK------DEVEGKSGRVEDEVEETYEDLPVIDLGLLKMGNLEREK 74
           MD +PP    YK L+       D     SG + ++  E + +LP+IDLG L +G++ERE 
Sbjct: 1   MDYEPPFLEFYKPLLLQGSHSVDVGISGSGIIRNDKSE-WRELPLIDLGQLSLGHVERED 60

Query: 75  CKKEIVEAARNWGFFQVMNHGVPERALKALLYEQKKVFYHPFAHKSLGNFLNLGG--TYR 134
           C +EI EAAR WGFFQV+NHGV +  L++L +EQ +VF  PFA KS  +FLNL    +YR
Sbjct: 61  CMREICEAARTWGFFQVVNHGVSQELLQSLRHEQVEVFRTPFARKSRESFLNLPAARSYR 120

Query: 135 WGNPLALSPSQISWSEAFHIAVLDVSSLEEHATLRSTMETVAKKFGSLAESIAEILGQSL 194
           WGNP A +  QISWSEAFH+ + D++ +++H +LRST+E  A     LAES+ +IL Q L
Sbjct: 121 WGNPSATNLRQISWSEAFHMFLPDIARMDQHQSLRSTIEAFASVVSPLAESLVQILVQKL 180

Query: 195 GIKWSYFKERCEKGKSSLRLNRYPPCP-FASKVYGLIPHTDSDYLTILYQAHTSGLQLMR 254
            IK+SYF+E C    S LRLNRYPPCP F S+V+GL+PHTDS +LTI+ Q    GLQ+M+
Sbjct: 181 NIKFSYFRENCSANTSFLRLNRYPPCPIFHSRVFGLLPHTDSSFLTIVNQDQIGGLQIMK 240

Query: 255 DGTWFPVKPNPQYLLVNIGDLLQVVSNDVFKSIKHRVVASEEDERFSFAYFYCPSEDVMI 314
           DG WF VKPNPQ L+VNIGDLLQ +SND++ S KHRVVA+E+ ERFS AYFY PS+D +I
Sbjct: 241 DGNWFGVKPNPQALVVNIGDLLQALSNDIYISAKHRVVAAEKVERFSVAYFYNPSKDALI 300

Query: 315 ESCMKPSIYKQFSYKEYRQQIEKDVEKTGNKVGLSRFFL 345
           ES + P +Y++F++ EYR+QIEKDV++TG+KVGLSRF L
Sbjct: 301 ESHIMPPMYRKFTFGEYRRQIEKDVKETGDKVGLSRFLL 338

[Close]

BLAST of CmoCh04G010010 vs. TrEMBL
Match: A0A0B2R2Q6_GLYSO (Gibberellin 2-beta-dioxygenase 8 OS=Glycine soja GN=glysoja_007702 PE=3 SV=1)

HSP 1 Score: 372.5 bits (955), Expect = 5.8e-100
Identity = 188/339 (55.46%), Postives = 247/339 (72.86%), Query Frame = 1


Query: 15  MDIDPPLQVCYKALMK------DEVEGKSGRVEDEVEETYEDLPVIDLGLLKMGNLEREK 74
           MD +PP    YK L+       D     SG + ++  E + +LP+IDLG L +G++ERE 
Sbjct: 1   MDYEPPFLEFYKPLLLQGSHSVDVGISGSGIIRNDKSE-WRELPLIDLGQLSLGHVERED 60

Query: 75  CKKEIVEAARNWGFFQVMNHGVPERALKALLYEQKKVFYHPFAHKSLGNFLNLGG--TYR 134
           C +EI EAAR WGFFQV+NHGV +  L++L +EQ +VF  PFA KS  +FLNL    +YR
Sbjct: 61  CMREICEAARTWGFFQVVNHGVSQELLQSLRHEQVEVFRTPFARKSRESFLNLPAARSYR 120

Query: 135 WGNPLALSPSQISWSEAFHIAVLDVSSLEEHATLRSTMETVAKKFGSLAESIAEILGQSL 194
           WGNP A +  QISWSEAFH+ + D++ +++H +LRST+E  A     LAES+ +IL Q L
Sbjct: 121 WGNPSATNLRQISWSEAFHMFLPDIARMDQHQSLRSTIEAFASVVSPLAESLVQILVQKL 180

Query: 195 GIKWSYFKERCEKGKSSLRLNRYPPCP-FASKVYGLIPHTDSDYLTILYQAHTSGLQLMR 254
            IK+SYF+E C    S LRLNRYPPCP F S+V+GL+PHTDS +LTI+ Q    GLQ+M+
Sbjct: 181 NIKFSYFRENCSANTSFLRLNRYPPCPIFHSRVFGLLPHTDSSFLTIVNQDQIGGLQIMK 240

Query: 255 DGTWFPVKPNPQYLLVNIGDLLQVVSNDVFKSIKHRVVASEEDERFSFAYFYCPSEDVMI 314
           DG WF VKPNPQ L+VNIGDLLQ +SND++ S KHRVVA+E+ ERFS AYFY PS+D +I
Sbjct: 241 DGNWFGVKPNPQALVVNIGDLLQALSNDIYISAKHRVVAAEKVERFSVAYFYNPSKDALI 300

Query: 315 ESCMKPSIYKQFSYKEYRQQIEKDVEKTGNKVGLSRFFL 345
           ES + P +Y++F++ EYR+QIEKDV++TG+KVGLSRF L
Sbjct: 301 ESHIMPPMYRKFTFGEYRRQIEKDVKETGDKVGLSRFLL 338

[Close]

BLAST of CmoCh04G010010 vs. TrEMBL
Match: A0A061FDJ5_THECC (Gibberellin 2-oxidase 8 OS=Theobroma cacao GN=TCM_031076 PE=3 SV=1)

HSP 1 Score: 370.5 bits (950), Expect = 2.2e-99
Identity = 176/329 (53.50%), Postives = 240/329 (72.95%), Query Frame = 1


Query: 15  MDIDPPLQVCYKALMKDEVEGKSGRVEDEVEETYEDLPVIDLGLLKMGNLEREKCKKEIV 74
           M ++PP    YKA+++   E +     +  EE   +LP+IDL  L +G  ER+KC +++ 
Sbjct: 1   MGVEPPFFEKYKAILQSSTEKEKFSTIEVFEEF--ELPLIDLSGLNLGPFERQKCIEQMD 60

Query: 75  EAARNWGFFQVMNHGVPERALKALLYEQKKVFYHPFAHKSLGNFLNLGG-TYRWGNPLAL 134
           +AAR WGFFQ++NHG+P   L  L YEQ KVF+ PF  K+   FLNL   +YRWGNPLA 
Sbjct: 61  KAAREWGFFQIVNHGIPGGLLDRLKYEQMKVFHLPFDKKAEKKFLNLSAHSYRWGNPLAT 120

Query: 135 SPSQISWSEAFHIAVLDVSSLEEHATLRSTMETVAKKFGSLAESIAEILGQSLGIKWSYF 194
           S   +SWSEA HI++ D+S ++E   LRST++  A+K   LA+ +AE L Q LGIK +YF
Sbjct: 121 SLRNLSWSEALHISLKDISRMDECNKLRSTIQEYARKANLLAQKLAEHLAQILGIKPNYF 180

Query: 195 KERCEKGKSSLRLNRYPPCPFASKVYGLIPHTDSDYLTILYQAHTSGLQLMRDGTWFPVK 254
           +E C    SSLR+NRYPPCPF+S+++G+IPHTD+D+LTIL Q    GLQ   DG WF VK
Sbjct: 181 QENCSPSSSSLRMNRYPPCPFSSRMFGIIPHTDTDFLTILSQDQVGGLQFNTDGRWFSVK 240

Query: 255 PNPQYLLVNIGDLLQVVSNDVFKSIKHRVVASEEDERFSFAYFYCPSEDVMIESCMKPSI 314
           PNP+ L++NIGDL Q +SN ++KSI HRV A+EE ER+S AYFYCP+++V+I+SC +P++
Sbjct: 241 PNPKALVINIGDLFQALSNGIYKSITHRVAANEEVERYSIAYFYCPTDEVVIQSCSEPAL 300

Query: 315 YKQFSYKEYRQQIEKDVEKTGNKVGLSRF 343
           Y+ FS+KEY+QQIE+DV+ TG+KVGLSRF
Sbjct: 301 YRNFSFKEYKQQIERDVKATGDKVGLSRF 327

[Close]

BLAST of CmoCh04G010010 vs. TrEMBL
Match: I1K1R2_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_05G081600 PE=3 SV=1)

HSP 1 Score: 370.5 bits (950), Expect = 2.2e-99
Identity = 184/332 (55.42%), Postives = 244/332 (73.49%), Query Frame = 1


Query: 15  MDIDPPLQVCYKALMKDEVEGKSGRVEDEVEETYEDLPVIDLGLLKMGNLEREKCKKEIV 74
           MD +PP    YKAL+++ V+      +  + E  E LPVIDLG     N ER++C+KEI 
Sbjct: 1   MDYEPPFLETYKALVQNHVDDSKN--DSSLVERCE-LPVIDLGKF---NYERDECEKEIA 60

Query: 75  EAARNWGFFQVMNHGVPERALKALLYEQKKVFYHPFAHKSLG-NFLNLGG-TYRWGNPLA 134
           EAA  WGFFQV+NHG+ +  LK+L +EQKK+FY PF +KS   NF +L   TYRWGNP A
Sbjct: 61  EAANKWGFFQVVNHGISQELLKSLEFEQKKLFYQPFVNKSAKFNFSSLSAKTYRWGNPFA 120

Query: 135 LSPSQISWSEAFHIAVLDVSSLEEHATLRSTMETVAKKFGSLAESIAEILGQSLGIKWSY 194
            +  Q+SWSEAFH  + D+S +++H ++RS++E  A +  SLA+S+AEIL  +L  K +Y
Sbjct: 121 TNLRQLSWSEAFHFYLSDISWMDQHHSMRSSLEAFASRVFSLAKSLAEILAFNLNTKSNY 180

Query: 195 FKERCEKGKSSLRLNRYPPCPFASKVYGLIPHTDSDYLTILYQAHTSGLQLMRDGTWFPV 254
           F+E C    S +RLNRYPPCP +SKV+GL+PH+D+ +LTI++Q    GLQLM+DG W  V
Sbjct: 181 FRENCLPKSSYIRLNRYPPCPISSKVHGLLPHSDTSFLTIVHQDQVGGLQLMKDGKWVGV 240

Query: 255 KPNPQYLLVNIGDLLQVVSNDVFKSIKHRVVASEEDERFSFAYFYCPSEDVMIESCMKPS 314
           KPNPQ L+VNIGD  Q  SN V+KSIKHRVVASE+ ERFS A+FYCPSE+ +IES +KP+
Sbjct: 241 KPNPQALVVNIGDFFQAFSNGVYKSIKHRVVASEKVERFSVAFFYCPSEEAVIESHIKPA 300

Query: 315 IYKQFSYKEYRQQIEKDVEKTGNKVGLSRFFL 345
            Y++F+ +EYRQQ EKDV++TG+KVGLSRF L
Sbjct: 301 TYRKFTSREYRQQTEKDVKQTGDKVGLSRFLL 326

[Close]

BLAST of CmoCh04G010010 vs. TrEMBL
Match: A0A0B2SGG6_GLYSO (Gibberellin 2-beta-dioxygenase 8 OS=Glycine soja GN=glysoja_047321 PE=3 SV=1)

HSP 1 Score: 370.5 bits (950), Expect = 2.2e-99
Identity = 184/332 (55.42%), Postives = 244/332 (73.49%), Query Frame = 1


Query: 15  MDIDPPLQVCYKALMKDEVEGKSGRVEDEVEETYEDLPVIDLGLLKMGNLEREKCKKEIV 74
           MD +PP    YKAL+++ V+      +  + E  E LPVIDLG     N ER++C+KEI 
Sbjct: 1   MDYEPPFLETYKALVQNHVDDSKN--DSSLVERCE-LPVIDLGKF---NYERDECEKEIA 60

Query: 75  EAARNWGFFQVMNHGVPERALKALLYEQKKVFYHPFAHKSLG-NFLNLGG-TYRWGNPLA 134
           EAA  WGFFQV+NHG+ +  LK+L +EQKK+FY PF +KS   NF +L   TYRWGNP A
Sbjct: 61  EAANKWGFFQVVNHGISQELLKSLEFEQKKLFYQPFVNKSAKFNFSSLSAKTYRWGNPFA 120

Query: 135 LSPSQISWSEAFHIAVLDVSSLEEHATLRSTMETVAKKFGSLAESIAEILGQSLGIKWSY 194
            +  Q+SWSEAFH  + D+S +++H ++RS++E  A +  SLA+S+AEIL  +L  K +Y
Sbjct: 121 TNLRQLSWSEAFHFYLSDISWMDQHHSMRSSLEAFASRVFSLAKSLAEILAFNLNTKSNY 180

Query: 195 FKERCEKGKSSLRLNRYPPCPFASKVYGLIPHTDSDYLTILYQAHTSGLQLMRDGTWFPV 254
           F+E C    S +RLNRYPPCP +SKV+GL+PH+D+ +LTI++Q    GLQLM+DG W  V
Sbjct: 181 FRENCLPKSSYIRLNRYPPCPISSKVHGLLPHSDTSFLTIVHQDQVGGLQLMKDGKWVGV 240

Query: 255 KPNPQYLLVNIGDLLQVVSNDVFKSIKHRVVASEEDERFSFAYFYCPSEDVMIESCMKPS 314
           KPNPQ L+VNIGD  Q  SN V+KSIKHRVVASE+ ERFS A+FYCPSE+ +IES +KP+
Sbjct: 241 KPNPQALVVNIGDFFQAFSNGVYKSIKHRVVASEKVERFSVAFFYCPSEEAVIESHIKPA 300

Query: 315 IYKQFSYKEYRQQIEKDVEKTGNKVGLSRFFL 345
            Y++F+ +EYRQQ EKDV++TG+KVGLSRF L
Sbjct: 301 TYRKFTSREYRQQTEKDVKQTGDKVGLSRFLL 326

[Close]

BLAST of CmoCh04G010010 vs. TAIR10
Match: AT4G21200.1 (AT4G21200.1 gibberellin 2-oxidase 8)

HSP 1 Score: 316.2 bits (809), Expect = 2.5e-86
Identity = 162/339 (47.79%), Postives = 217/339 (64.01%), Query Frame = 1


Query: 17  IDPPLQVCYKALMKDEVEGKSGRVED---------EVEETYEDLPVIDLGLLKMG-NLER 76
           +DPP    Y  L+ +++  K   V +          VEE   +LPVID+  L  G   ER
Sbjct: 1   MDPPFNEIYNNLLYNQITKKDNDVSEIPFSFSVTAVVEEV--ELPVIDVSRLIDGAEEER 60

Query: 77  EKCKKEIVEAARNWGFFQVMNHGVPERALKALLYEQKKVFYHPFAHKSLGNFLNLGGTYR 136
           EKCK+ I  A+R WGFFQV+NHG+    L+ +  EQ +VF  PF  KS     +  G+YR
Sbjct: 61  EKCKEAIARASREWGFFQVINHGISMDVLEKMRQEQIRVFREPFDKKSKSEKFS-AGSYR 120

Query: 137 WGNPLALSPSQISWSEAFHIAVLDVSSLEEHATLRSTMETVAKKFGSLAESIAEILGQSL 196
           WG P A S  Q+SWSEAFH+ + D+S  ++  TL STME  A +  +LA  +AE+L +  
Sbjct: 121 WGTPSATSIRQLSWSEAFHVPMTDISDNKDFTTLSSTMEKFASESEALAYMLAEVLAEKS 180

Query: 197 GIKWSYFKERCEKGKSSLRLNRYPPCPFASKVYGLIPHTDSDYLTILYQAHTSGLQLMRD 256
           G   S+FKE C +    LR+NRYPPCP  S+VYGL+PHTDSD+LTILYQ    GLQL++D
Sbjct: 181 GQNSSFFKENCVRNTCYLRMNRYPPCPKPSEVYGLMPHTDSDFLTILYQDQVGGLQLIKD 240

Query: 257 GTWFPVKPNPQYLLVNIGDLLQVVSNDVFKSIKHRVVASEEDERFSFAYFYCPSEDVMIE 316
             W  VKPNP+ L++NIGDL Q  SN ++KS++HRV+ + + ERFS AYF CPS D +IE
Sbjct: 241 NRWIAVKPNPKALIINIGDLFQAWSNGMYKSVEHRVMTNPKVERFSTAYFMCPSYDAVIE 300

Query: 317 SCMKPSIYKQFSYKEYRQQIEKDVEKTGNKVGLSRFFLH 346
                  Y+ FS++E+RQQ+++DV+K G KVGL RF  H
Sbjct: 301 CSSDRPAYRNFSFREFRQQVQEDVKKFGFKVGLPRFLNH 336

[Close]

BLAST of CmoCh04G010010 vs. TAIR10
Match: AT1G50960.1 (AT1G50960.1 gibberellin 2-oxidase 7)

HSP 1 Score: 273.5 bits (698), Expect = 1.9e-73
Identity = 135/297 (45.45%), Postives = 199/297 (67.00%), Query Frame = 1


Query: 51  LPVIDLGLLKMGN-LEREKCKKEIVEAARNWGFFQVMNHGVPERALKALLYEQKKVFYHP 110
           LPVIDL  L  G  ++R++C K++V AA+ WGFFQ++NHG+P+   + +L E+KK+F  P
Sbjct: 40  LPVIDLSHLTSGEEVKRKRCVKQMVAAAKEWGFFQIVNHGIPKDVFEMMLLEEKKLFDQP 99

Query: 111 FAHKSLGNFLNLG-GTYRWGNPLALSPSQISWSEAFHIAVLDVSSL-EEHATLRSTMETV 170
           F+ K    F +L   +YRWGNP A SP+Q S SEAFHI + +VS + ++   LR+ +ET 
Sbjct: 100 FSVKVRERFSDLSKNSYRWGNPSATSPAQYSVSEAFHIILSEVSRISDDRNNLRTIVETY 159

Query: 171 AKKFGSLAESIAEILGQSLGIKWSYFKERCEKGKSSLRLNRYPPCPFASKVYGLIPHTDS 230
            ++   +A+ I EILG+ + +   YF+   E   S LRLN+Y P  F S+V+GL+PHTD+
Sbjct: 160 VQEIARVAQMICEILGKQVNVSSEYFENIFELENSFLRLNKYHPSVFGSEVFGLVPHTDT 219

Query: 231 DYLTILYQAHTSGLQLMRDGTWFPVKPNPQYLLVNIGDLLQVVSNDVFKSIKHRVVASEE 290
            +LTIL Q    GL+L  +G W  VKP  + L VNIGD+ Q +SN V++S++HRV++   
Sbjct: 220 SFLTILSQDQIGGLELENNGQWISVKPCLEALTVNIGDMFQALSNGVYQSVRHRVISPAN 279

Query: 291 DERFSFAYFYCPSEDVMIESCMKPSIYKQFSYKEYRQQIEKDVEKTGNKVGLSRFFL 345
            ER S A+F CP  +  I+    P  Y++FS++EY++Q E DV++TG+KVGLSRF +
Sbjct: 280 IERMSIAFFVCPYLETEIDCFGYPKKYRRFSFREYKEQSEHDVKETGDKVGLSRFLI 336

[Close]

BLAST of CmoCh04G010010 vs. TAIR10
Match: AT5G05600.1 (AT5G05600.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 159.1 bits (401), Expect = 5.1e-39
Identity = 107/347 (30.84%), Postives = 169/347 (48.70%), Query Frame = 1


Query: 4   LKAETELKQLSMDIDPPLQVCYKALMKDEVEGKSGR---------VEDEVEETYEDLPVI 63
           +  + E K+ SMD  P   V  ++L +  +     R            E   T  ++P+I
Sbjct: 6   IDVKIETKKGSMDEWPEPIVRVQSLAESNLSSLPDRYIKPASLRPTTTEDAPTATNIPII 65

Query: 64  DL-GLLKMGNLEREKCKKEIVEAARNWGFFQVMNHGVPERALKALLYEQKKVFYHPFAHK 123
           DL GL     L  +     I EA R WGFFQV+NHGV    + A     ++ F+ P   K
Sbjct: 66  DLEGLFSEEGLSDDVIMARISEACRGWGFFQVVNHGVKPELMDAARENWREFFHMPVNAK 125

Query: 124 SLGNFLNLGGTYR-WGNPLALSP-SQISWSEAFHIAVLDVSSLEEHA------TLRSTME 183
               + N   TY  +G+ L +   + + WS+ + + +L     + +       T+R  ++
Sbjct: 126 E--TYSNSPRTYEGYGSRLGVEKGASLDWSDYYFLHLLPHHLKDFNKWPSFPPTIREVID 185

Query: 184 TVAKKFGSLAESIAEILGQSLGIKWSYFKERC--EKGKSSLRLNRYPPCPFASKVYGLIP 243
              ++   L+  I  +L  +LG+K   F+E    E   + LR+N YP CP      GL P
Sbjct: 186 EYGEELVKLSGRIMRVLSTNLGLKEDKFQEAFGGENIGACLRVNYYPKCPRPELALGLSP 245

Query: 244 HTDSDYLTILY-QAHTSGLQLMRDGTWFPVKPNPQYLLVNIGDLLQVVSNDVFKSIKHRV 303
           H+D   +TIL       GLQ+ +D TW  VKP+P   +VNIGD +Q++SN  +KS++HRV
Sbjct: 246 HSDPGGMTILLPDDQVFGLQVRKDDTWITVKPHPHAFIVNIGDQIQILSNSTYKSVEHRV 305

Query: 304 VASEEDERFSFAYFYCPSEDVMIE------SCMKPSIYKQFSYKEYR 324
           + + + ER S A+FY P  D+ I+      S   P +Y   ++ +YR
Sbjct: 306 IVNSDKERVSLAFFYNPKSDIPIQPLQELVSTHNPPLYPPMTFDQYR 350

[Close]

BLAST of CmoCh04G010010 vs. TAIR10
Match: AT4G10490.1 (AT4G10490.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 154.8 bits (390), Expect = 9.7e-38
Identity = 89/294 (30.27%), Postives = 140/294 (47.62%), Query Frame = 1


Query: 43  EVEETYEDLPVIDLGLLKMGNLEREKCKKEIVEAARNWGFFQVMNHGVPERALKALLYEQ 102
           EV+ + + +P+IDL  L   N  R     +   A  + GFFQ+ NHGVPE  +K ++   
Sbjct: 34  EVQTSGDSIPLIDLHDLHGPN--RADIINQFAHACSSCGFFQIKNHGVPEETIKKMMNAA 93

Query: 103 KKVFYHPFAHKSLGNFLNLGGTYRWGNPLALSPSQIS-WSEAFHIAVLDVSSLEEH---- 162
           ++ F    + +      +   T R      +S  ++S W +   +    +          
Sbjct: 94  REFFRQSESERVKHYSADTKKTTRLSTSFNVSKEKVSNWRDFLRLHCYPIEDFINEWPST 153

Query: 163 -ATLRSTMETVAKKFGSLAESIAEILGQSLGIKWSYFKERCEKGKSSLRLNRYPPCPFAS 222
             + R      A    +L  ++ E + +SLG+          K    + +N YP CP   
Sbjct: 154 PISFREVTAEYATSVRALVLTLLEAISESLGLAKDRVSNTIGKHGQHMAINYYPRCPQPE 213

Query: 223 KVYGLIPHTDSDYLTILYQAHTSGLQLMRDGTWFPVKPNPQYLLVNIGDLLQVVSNDVFK 282
             YGL  H D++ +T+L Q   SGLQ+ +DG W  V P P   +VN+GD +QV+SN+ +K
Sbjct: 214 LTYGLPGHKDANLITVLLQDEVSGLQVFKDGKWIAVNPVPNTFIVNLGDQMQVISNEKYK 273

Query: 283 SIKHRVVASEEDERFSFAYFYCPSEDVMIESCMK--------PSIYKQFSYKEY 323
           S+ HR V + + ER S   FYCPSED +I    +        P+IY+ F+Y EY
Sbjct: 274 SVLHRAVVNSDMERISIPTFYCPSEDAVISPAQELINEEEDSPAIYRNFTYAEY 325

[Close]

BLAST of CmoCh04G010010 vs. TAIR10
Match: AT1G62380.1 (AT1G62380.1 ACC oxidase 2)

HSP 1 Score: 152.5 bits (384), Expect = 4.8e-37
Identity = 90/283 (31.80%), Postives = 142/283 (50.18%), Query Frame = 1


Query: 52  PVIDLGLLKMGNLEREKCKKEIVEAARNWGFFQVMNHGVPERALKALLYEQKKVFYHPFA 111
           PV+DL   K+   ER++    I EA  NWGFF+++NHG+P   +  +  +  K  Y    
Sbjct: 8   PVVDLS--KLNGEERDQTMALINEACENWGFFEIVNHGLPHDLMDKI-EKMTKDHYKTCQ 67

Query: 112 HKSLGNFLNLGGTYRWGNPLALSPSQISWSEAFHIAVLDVSSLEEHATLRSTMETVAKKF 171
            +   + L   G     + L      + W   F++  L  S+L + + +     T  K F
Sbjct: 68  EQKFNDMLKSKGL----DNLETEVEDVDWESTFYVRHLPQSNLNDISDVSDEYRTAMKDF 127

Query: 172 G----SLAESIAEILGQSLGIKWSYFKERCEKGKS---SLRLNRYPPCPFASKVYGLIPH 231
           G    +LAE + ++L ++LG++  Y K+     K      +++ YPPCP    + GL  H
Sbjct: 128 GKRLENLAEDLLDLLCENLGLEKGYLKKVFHGTKGPTFGTKVSNYPPCPKPEMIKGLRAH 187

Query: 232 TDSDYLTILYQAH-TSGLQLMRDGTWFPVKPNPQYLLVNIGDLLQVVSNDVFKSIKHRVV 291
           TD+  + +L+Q    SGLQL++DG W  V P    +++N+GD L+V++N  +KS+ HRVV
Sbjct: 188 TDAGGIILLFQDDKVSGLQLLKDGDWIDVPPLNHSIVINLGDQLEVITNGKYKSVLHRVV 247

Query: 292 ASEEDERFSFAYFYCPSEDVMIESCM----KPSIYKQFSYKEY 323
             +E  R S A FY P  D  I        K S Y  F + +Y
Sbjct: 248 TQQEGNRMSVASFYNPGSDAEISPATSLVEKDSEYPSFVFDDY 283

[Close]

BLAST of CmoCh04G010010 vs. NCBI nr
Match: gi|659126970|ref|XP_008463453.1| (PREDICTED: gibberellin 2-beta-dioxygenase 8-like [Cucumis melo])

HSP 1 Score: 499.2 bits (1284), Expect = 5.9e-138
Identity = 250/340 (73.53%), Postives = 285/340 (83.82%), Query Frame = 1


Query: 15  MDIDPPLQVCYKALMKDEVEGKSGRVEDE--VEETYED--LPVIDLGLLKMGNLEREKCK 74
           M+I+PPL+  Y  L K+E+ G+     DE  +EE  E+  LPVIDLG LK GNLERE+CK
Sbjct: 1   MEIEPPLEERYNELKKEELNGRRIIEADEEAIEECEEEIELPVIDLGQLKKGNLEREECK 60

Query: 75  KEIVEAARNWGFFQVMNHGVPERALKALLYEQKKVFYHPFAHKSL-GNFLNLGGT-YRWG 134
           KEIVEAA NWGFFQV+NHGV E+ L A++ EQKKVF  PF +KSL GNFLNL  T YRWG
Sbjct: 61  KEIVEAAMNWGFFQVINHGVAEKVLNAMINEQKKVFNQPFVNKSLSGNFLNLPSTHYRWG 120

Query: 135 NPLALSPSQISWSEAFHIAVLDVSSLEEHATLRSTMETVAKKFGSLAESIAEILGQSLGI 194
           NP+A+S SQISWSEAFHI VL+VS+L  H TLR+TME V KK GSLAE I+EILGQSLGI
Sbjct: 121 NPVAISSSQISWSEAFHIPVLEVSTLNHHITLRTTMEGVVKKLGSLAEKISEILGQSLGI 180

Query: 195 KWSYFKERCEKGKSSLRLNRYPPCPFASKVYGLIPHTDSDYLTILYQAHTSGLQLMRDGT 254
           K +YFKERCEKGKSS R+NRYPPCP AS+VYGLIPHTD+DYLTILYQ   SGLQLM+   
Sbjct: 181 KSNYFKERCEKGKSSFRMNRYPPCPIASQVYGLIPHTDTDYLTILYQPQISGLQLMKSAK 240

Query: 255 WFPVKPNPQYLLVNIGDLLQVVSNDVFKSIKHRVVASEEDERFSFAYFYCPSEDVMIESC 314
           WFPVKPNPQ LL+NIGDL QVVSND+F+S+KHRVVAS   ER+SFAYFYCPS+DVMIES 
Sbjct: 241 WFPVKPNPQALLLNIGDLFQVVSNDIFRSLKHRVVASVGVERYSFAYFYCPSDDVMIESW 300

Query: 315 MKPSIYKQFSYKEYRQQIEKDVEKTGNKVGLSRFFLHHML 349
           +KPSIYKQFSYKEYRQQIEKDVEKTGNKVGLSRFFLH+++
Sbjct: 301 LKPSIYKQFSYKEYRQQIEKDVEKTGNKVGLSRFFLHNII 340

[Close]

BLAST of CmoCh04G010010 vs. NCBI nr
Match: gi|778676167|ref|XP_004152933.2| (PREDICTED: gibberellin 2-beta-dioxygenase 8-like [Cucumis sativus])

HSP 1 Score: 489.6 bits (1259), Expect = 4.7e-135
Identity = 245/338 (72.49%), Postives = 280/338 (82.84%), Query Frame = 1


Query: 16  DIDPPLQVCYKALMKDEVEGKSGRVEDE--VEETYED--LPVIDLGLLKMGNLEREKCKK 75
           +I+PPL+  Y  L K+E+ G+     DE  +EE  E+  LPVIDLG LK GNLEREKCKK
Sbjct: 3   EIEPPLEERYNELKKEELNGRRTIEADEEVIEECEEEIELPVIDLGQLKKGNLEREKCKK 62

Query: 76  EIVEAARNWGFFQVMNHGVPERALKALLYEQKKVFYHPFAHKSLG-NFLNLGGT-YRWGN 135
           EIVEAA NWGFFQV+NHGV E+ L A++ EQKKVF  PF +KSL  NFLNL  T YRWGN
Sbjct: 63  EIVEAAMNWGFFQVINHGVAEKVLNAMINEQKKVFNQPFVNKSLSTNFLNLPSTHYRWGN 122

Query: 136 PLALSPSQISWSEAFHIAVLDVSSLEEHATLRSTMETVAKKFGSLAESIAEILGQSLGIK 195
           P+A+S SQISWSEAFHI VL+VS+   H TLR+TME V KK GSLAE I+EILGQSLGIK
Sbjct: 123 PVAISSSQISWSEAFHIPVLEVSTSHHHITLRTTMEGVVKKLGSLAEKISEILGQSLGIK 182

Query: 196 WSYFKERCEKGKSSLRLNRYPPCPFASKVYGLIPHTDSDYLTILYQAHTSGLQLMRDGTW 255
            +YFKERCEKGKSS R+NRYPPCP AS+VYGLIPHTD+DYLTILYQ   SGLQL +   W
Sbjct: 183 SNYFKERCEKGKSSFRMNRYPPCPIASQVYGLIPHTDTDYLTILYQPQISGLQLKKSAKW 242

Query: 256 FPVKPNPQYLLVNIGDLLQVVSNDVFKSIKHRVVASEEDERFSFAYFYCPSEDVMIESCM 315
           FPVKPNP+ LL+NIGDL QVVSND+F+S+KHRVVASE  ER+SFAYFYCPS+DV+IES +
Sbjct: 243 FPVKPNPRALLLNIGDLFQVVSNDIFRSLKHRVVASEGVERYSFAYFYCPSDDVIIESWL 302

Query: 316 KPSIYKQFSYKEYRQQIEKDVEKTGNKVGLSRFFLHHM 348
           KPSIYK FSYKEYRQQIEKDVEKTGNKVGLSRFFLH++
Sbjct: 303 KPSIYKHFSYKEYRQQIEKDVEKTGNKVGLSRFFLHNI 340

[Close]

BLAST of CmoCh04G010010 vs. NCBI nr
Match: gi|356542211|ref|XP_003539563.1| (PREDICTED: gibberellin 2-beta-dioxygenase 8-like [Glycine max])

HSP 1 Score: 372.5 bits (955), Expect = 8.4e-100
Identity = 188/339 (55.46%), Postives = 247/339 (72.86%), Query Frame = 1


Query: 15  MDIDPPLQVCYKALMK------DEVEGKSGRVEDEVEETYEDLPVIDLGLLKMGNLEREK 74
           MD +PP    YK L+       D     SG + ++  E + +LP+IDLG L +G++ERE 
Sbjct: 1   MDYEPPFLEFYKPLLLQGSHSVDVGISGSGIIRNDKSE-WRELPLIDLGQLSLGHVERED 60

Query: 75  CKKEIVEAARNWGFFQVMNHGVPERALKALLYEQKKVFYHPFAHKSLGNFLNLGG--TYR 134
           C +EI EAAR WGFFQV+NHGV +  L++L +EQ +VF  PFA KS  +FLNL    +YR
Sbjct: 61  CMREICEAARTWGFFQVVNHGVSQELLQSLRHEQVEVFRTPFARKSRESFLNLPAARSYR 120

Query: 135 WGNPLALSPSQISWSEAFHIAVLDVSSLEEHATLRSTMETVAKKFGSLAESIAEILGQSL 194
           WGNP A +  QISWSEAFH+ + D++ +++H +LRST+E  A     LAES+ +IL Q L
Sbjct: 121 WGNPSATNLRQISWSEAFHMFLPDIARMDQHQSLRSTIEAFASVVSPLAESLVQILVQKL 180

Query: 195 GIKWSYFKERCEKGKSSLRLNRYPPCP-FASKVYGLIPHTDSDYLTILYQAHTSGLQLMR 254
            IK+SYF+E C    S LRLNRYPPCP F S+V+GL+PHTDS +LTI+ Q    GLQ+M+
Sbjct: 181 NIKFSYFRENCSANTSFLRLNRYPPCPIFHSRVFGLLPHTDSSFLTIVNQDQIGGLQIMK 240

Query: 255 DGTWFPVKPNPQYLLVNIGDLLQVVSNDVFKSIKHRVVASEEDERFSFAYFYCPSEDVMI 314
           DG WF VKPNPQ L+VNIGDLLQ +SND++ S KHRVVA+E+ ERFS AYFY PS+D +I
Sbjct: 241 DGNWFGVKPNPQALVVNIGDLLQALSNDIYISAKHRVVAAEKVERFSVAYFYNPSKDALI 300

Query: 315 ESCMKPSIYKQFSYKEYRQQIEKDVEKTGNKVGLSRFFL 345
           ES + P +Y++F++ EYR+QIEKDV++TG+KVGLSRF L
Sbjct: 301 ESHIMPPMYRKFTFGEYRRQIEKDVKETGDKVGLSRFLL 338

[Close]

BLAST of CmoCh04G010010 vs. NCBI nr
Match: gi|1000957715|ref|XP_002522845.2| (PREDICTED: gibberellin 2-beta-dioxygenase 8 [Ricinus communis])

HSP 1 Score: 372.1 bits (954), Expect = 1.1e-99
Identity = 175/333 (52.55%), Postives = 242/333 (72.67%), Query Frame = 1


Query: 13  LSMDIDPPLQVCYKALMKDEVEGKSGRVEDEVEETYEDLPVIDLGLLKMGNLEREKCKKE 72
           +SMDIDPP Q  ++AL K+ +  ++   ED +  +  +LP+ID+  L +G+ +REKC KE
Sbjct: 19  ISMDIDPPFQEKHRALFKNSIVRETN--EDMLMASQCELPLIDMNSLNVGHPDREKCIKE 78

Query: 73  IVEAARNWGFFQVMNHGVPERALKALLYEQKKVFYHPFAHKSLGNFLNL-GGTYRWGNPL 132
           + EAA  WGFFQV+NHG+P   L+ L YEQ  VF+ PF+ KS   FLNL   +Y WGN  
Sbjct: 79  MGEAASEWGFFQVVNHGIPNEILERLQYEQMMVFHEPFSKKSQDRFLNLPANSYIWGNSK 138

Query: 133 ALSPSQISWSEAFHIAVLDVSSLEEHATLRSTMETVAKKFGSLAESIAEILGQSLGIKWS 192
           A   SQ SWSEAFHI V D+S+++    L ST+E       SLAES+AE L  ++G+K +
Sbjct: 139 ATCLSQFSWSEAFHIPVTDISTMDGVRNLGSTIEAFVNLAASLAESLAETLADNIGVKTN 198

Query: 193 YFKERCEKGKSSLRLNRYPPCPFASKVYGLIPHTDSDYLTILYQAHTSGLQLMRDGTWFP 252
           +FKE C    S +R+NRYPPCPF+S+VYGL+PHTDS++LTILYQ    GL+L++DG W  
Sbjct: 199 FFKENCTTSSSYVRMNRYPPCPFSSQVYGLLPHTDSNFLTILYQDQNGGLELLKDGNWLS 258

Query: 253 VKPNPQYLLVNIGDLLQVVSNDVFKSIKHRVVASEEDERFSFAYFYCPSEDVMIESCMKP 312
           V+PNP+ L++NIGD  Q  SN++++S +HRVVA ++ ERFS A+FYCPS + +IES +KP
Sbjct: 259 VRPNPEALVINIGDFFQAFSNNIYRSTEHRVVAPQKVERFSMAFFYCPSYEAVIESYIKP 318

Query: 313 SIYKQFSYKEYRQQIEKDVEKTGNKVGLSRFFL 345
           + Y++FS++EY+QQI+KDV+ TGNK+GLSRF L
Sbjct: 319 AKYRKFSFREYKQQIQKDVQATGNKIGLSRFLL 349

[Close]

BLAST of CmoCh04G010010 vs. NCBI nr
Match: gi|590607616|ref|XP_007021034.1| (Gibberellin 2-oxidase 8 [Theobroma cacao])

HSP 1 Score: 370.5 bits (950), Expect = 3.2e-99
Identity = 176/329 (53.50%), Postives = 240/329 (72.95%), Query Frame = 1


Query: 15  MDIDPPLQVCYKALMKDEVEGKSGRVEDEVEETYEDLPVIDLGLLKMGNLEREKCKKEIV 74
           M ++PP    YKA+++   E +     +  EE   +LP+IDL  L +G  ER+KC +++ 
Sbjct: 1   MGVEPPFFEKYKAILQSSTEKEKFSTIEVFEEF--ELPLIDLSGLNLGPFERQKCIEQMD 60

Query: 75  EAARNWGFFQVMNHGVPERALKALLYEQKKVFYHPFAHKSLGNFLNLGG-TYRWGNPLAL 134
           +AAR WGFFQ++NHG+P   L  L YEQ KVF+ PF  K+   FLNL   +YRWGNPLA 
Sbjct: 61  KAAREWGFFQIVNHGIPGGLLDRLKYEQMKVFHLPFDKKAEKKFLNLSAHSYRWGNPLAT 120

Query: 135 SPSQISWSEAFHIAVLDVSSLEEHATLRSTMETVAKKFGSLAESIAEILGQSLGIKWSYF 194
           S   +SWSEA HI++ D+S ++E   LRST++  A+K   LA+ +AE L Q LGIK +YF
Sbjct: 121 SLRNLSWSEALHISLKDISRMDECNKLRSTIQEYARKANLLAQKLAEHLAQILGIKPNYF 180

Query: 195 KERCEKGKSSLRLNRYPPCPFASKVYGLIPHTDSDYLTILYQAHTSGLQLMRDGTWFPVK 254
           +E C    SSLR+NRYPPCPF+S+++G+IPHTD+D+LTIL Q    GLQ   DG WF VK
Sbjct: 181 QENCSPSSSSLRMNRYPPCPFSSRMFGIIPHTDTDFLTILSQDQVGGLQFNTDGRWFSVK 240

Query: 255 PNPQYLLVNIGDLLQVVSNDVFKSIKHRVVASEEDERFSFAYFYCPSEDVMIESCMKPSI 314
           PNP+ L++NIGDL Q +SN ++KSI HRV A+EE ER+S AYFYCP+++V+I+SC +P++
Sbjct: 241 PNPKALVINIGDLFQALSNGIYKSITHRVAANEEVERYSIAYFYCPTDEVVIQSCSEPAL 300

Query: 315 YKQFSYKEYRQQIEKDVEKTGNKVGLSRF 343
           Y+ FS+KEY+QQIE+DV+ TG+KVGLSRF
Sbjct: 301 YRNFSFKEYKQQIERDVKATGDKVGLSRF 327

The following BLAST results are available for this feature:

BLAST of CmoCh04G010010 vs. Swiss-Prot


Match Name	E-value	Identity	Description
G2OX8_ARATH	4.5e-85	47.79	Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana GN=GA2OX8 PE=1 SV=2	[more]
G2OX7_ARATH	3.3e-72	45.45	Gibberellin 2-beta-dioxygenase 7 OS=Arabidopsis thaliana GN=GA2OX7 PE=1 SV=1	[more]
DLO2_ARATH	1.7e-36	30.27	Protein DMR6-LIKE OXYGENASE 2 OS=Arabidopsis thaliana GN=DLO2 PE=2 SV=1	[more]
ACCO2_ARATH	8.5e-36	31.80	1-aminocyclopropane-1-carboxylate oxidase 2 OS=Arabidopsis thaliana GN=ACO2 PE=2...	[more]
ACCO3_PETHY	2.7e-34	30.69	1-aminocyclopropane-1-carboxylate oxidase 3 OS=Petunia hybrida GN=ACO3 PE=3 SV=1	[more]

BLAST of CmoCh04G010010 vs. TrEMBL


Match Name	E-value	Identity	Description
K7LW87_SOYBN	5.8e-100	55.46	Uncharacterized protein OS=Glycine max GN=GLYMA_12G214000 PE=3 SV=1	[more]
A0A0B2R2Q6_GLYSO	5.8e-100	55.46	Gibberellin 2-beta-dioxygenase 8 OS=Glycine soja GN=glysoja_007702 PE=3 SV=1	[more]
A0A061FDJ5_THECC	2.2e-99	53.50	Gibberellin 2-oxidase 8 OS=Theobroma cacao GN=TCM_031076 PE=3 SV=1	[more]
I1K1R2_SOYBN	2.2e-99	55.42	Uncharacterized protein OS=Glycine max GN=GLYMA_05G081600 PE=3 SV=1	[more]
A0A0B2SGG6_GLYSO	2.2e-99	55.42	Gibberellin 2-beta-dioxygenase 8 OS=Glycine soja GN=glysoja_047321 PE=3 SV=1	[more]

BLAST of CmoCh04G010010 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G21200.1	2.5e-86	47.79	gibberellin 2-oxidase 8	[more]
AT1G50960.1	1.9e-73	45.45	gibberellin 2-oxidase 7	[more]
AT5G05600.1	5.1e-39	30.84	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot...	[more]
AT4G10490.1	9.7e-38	30.27	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot...	[more]
AT1G62380.1	4.8e-37	31.80	ACC oxidase 2	[more]

BLAST of CmoCh04G010010 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659126970\|ref\|XP_008463453.1\|	5.9e-138	73.53	PREDICTED: gibberellin 2-beta-dioxygenase 8-like [Cucumis melo]	[more]
gi\|778676167\|ref\|XP_004152933.2\|	4.7e-135	72.49	PREDICTED: gibberellin 2-beta-dioxygenase 8-like [Cucumis sativus]	[more]
gi\|356542211\|ref\|XP_003539563.1\|	8.4e-100	55.46	PREDICTED: gibberellin 2-beta-dioxygenase 8-like [Glycine max]	[more]
gi\|1000957715\|ref\|XP_002522845.2\|	1.1e-99	52.55	PREDICTED: gibberellin 2-beta-dioxygenase 8 [Ricinus communis]	[more]
gi\|590607616\|ref\|XP_007021034.1\|	3.2e-99	53.50	Gibberellin 2-oxidase 8 [Theobroma cacao]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR005123	Oxoglu/Fe-dep_dioxygenase
IPR026992	DIOX_N
IPR027443	IPNS-like

Vocabulary: **Molecular Function**
Term	Definition
GO:0016491	oxidoreductase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	oxidation-reduction process

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0071704	organic substance metabolic process
biological_process	GO:0055114	oxidation-reduction process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0016706	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
molecular_function	GO:0016491	oxidoreductase activity
molecular_function	GO:0051213	dioxygenase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh04G010010.1	CmoCh04G010010.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata
Date Performed: 2017-05-19


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PFAM	PF03171	2OG-FeII_Oxy	coord: 203..299 score: 3.4
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROFILE	PS51471	FE2OG_OXY	coord: 200..299 score: 12
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 51..149 score: 3.6
IPR027443	Isopenicillin N synthase-like	GENE3D	G3DSA:2.60.120.330		coord: 45..326 score: 1.6
None	No IPR available	PANTHER	PTHR10209	OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEIN	coord: 2..337 score: 6.2E
None	No IPR available	PANTHER	PTHR10209:SF177	SUBFAMILY NOT NAMED	coord: 2..337 score: 6.2E
None	No IPR available	unknown	SSF51197	Clavaminate synthase-like	coord: 48..326 score: 3.78

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CmoCh04G010010	Cucsa.059620	Cucumber (Gy14) v1	cgycmoB0076
CmoCh04G010010	CmaCh16G008420	Cucurbita maxima (Rimu)	cmacmoB337
CmoCh04G010010	CmaCh04G009310	Cucurbita maxima (Rimu)	cmacmoB728
CmoCh04G010010	Cla005259	Watermelon (97103) v1	cmowmB653
CmoCh04G010010	Csa3G081390	Cucumber (Chinese Long) v2	cmocuB693
CmoCh04G010010	MELO3C025434	Melon (DHL92) v3.5.1	cmomeB684
CmoCh04G010010	ClCG10G005830	Watermelon (Charleston Gray)	cmowcgB615
CmoCh04G010010	CSPI03G05430	Wild cucumber (PI 183967)	cmocpiB703
CmoCh04G010010	Lsi05G006390	Bottle gourd (USVL1VR-Ls)	cmolsiB684
CmoCh04G010010	Cp4.1LG00g00200	Cucurbita pepo (Zucchini)	cmocpeB644
CmoCh04G010010	Cp4.1LG01g08420	Cucurbita pepo (Zucchini)	cmocpeB673
CmoCh04G010010	MELO3C025434.2	Melon (DHL92) v3.6.1	cmomedB776
CmoCh04G010010	CsaV3_3G005490	Cucumber (Chinese Long) v3	cmocucB0822
CmoCh04G010010	Cla97C10G190000	Watermelon (97103) v2	cmowmbB689
CmoCh04G010010	Bhi11G000532	Wax gourd	cmowgoB0817
CmoCh04G010010	CsGy3G005550	Cucumber (Gy14) v2	cgybcmoB393
CmoCh04G010010	Carg24829	Silver-seed gourd	carcmoB1070
CmoCh04G010010	Carg00048	Silver-seed gourd	carcmoB0923

The following gene(s) are paralogous to this gene:


Gene	Paralogue	Organism	Block
CmoCh04G010010	CmoCh16G009200	Cucurbita moschata (Rifu)	cmocmoB290

The following block(s) are covering this gene:


Gene	Organism	Block
CmoCh04G010010	Cucurbita moschata (Rifu)	cmocmoB285
CmoCh04G010010	Bottle gourd (USVL1VR-Ls)	cmolsiB678
CmoCh04G010010	Bottle gourd (USVL1VR-Ls)	cmolsiB682