CSPI03G05430 (gene) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI03G05430
Type	gene
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	Gibberellin 2-beta-dioxygenase 8
Location	Chr3 : 4479894 .. 4480409 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCGAGATTGAACCACCATTAGAAGAAAGGTATAATGAGTTGAAGAAAGAGGAATTAAATGGCAGAAGAACTATAGAAGCTGATGAAGAAGTAATAGAAGAATGTGAAGAAGAAATAGAGTTACCAGTGATTGATTTAGGGCAACTAAAGAAGGGGAATTTAGAGAGAGAGAAATGCAAGAAAGAAATAGTGGAAGCAGCAATGAATTGGGGTTTTTTTCAAGTAATAAATCATGGGGTTGCTGAGAAAGTATTGAATGCAATGATAAATGAGCAAAAGAAAGTGTTTAATCAACCTTTTGTTAACAAAAGTTTGAGTACTAATTTTCTTAATTTGCCTTCAACTCACTACCGCTGGGGTAACCCTGTCGCTATTTCTTCCTCTCAAATCTCTTGGTCTGAAGCTTTTCATATTCCTGTTTCGGAAGTTTCAACCTCGCACCATCATATTACTCTCAGGTATGCCCTCTACCTTACTGTCCCTTCTTCACTTTCTCTTCGTTATCAACTCTGA

mRNA sequence

ATGGCCGAGATTGAACCACCATTAGAAGAAAGGTATAATGAGTTGAAGAAAGAGGAATTAAATGGCAGAAGAACTATAGAAGCTGATGAAGAAGTAATAGAAGAATGTGAAGAAGAAATAGAGTTACCAGTGATTGATTTAGGGCAACTAAAGAAGGGGAATTTAGAGAGAGAGAAATGCAAGAAAGAAATAGTGGAAGCAGCAATGAATTGGGGTTTTTTTCAAGTAATAAATCATGGGGTTGCTGAGAAAGTATTGAATGCAATGATAAATGAGCAAAAGAAAGTGTTTAATCAACCTTTTGTTAACAAAAGTTTGAGTACTAATTTTCTTAATTTGCCTTCAACTCACTACCGCTGGGGTAACCCTGTCGCTATTTCTTCCTCTCAAATCTCTTGGTCTGAAGCTTTTCATATTCCTGTTTCGGAAGTTTCAACCTCGCACCATCATATTACTCTCAGGTATGCCCTCTACCTTACTGTCCCTTCTTCACTTTCTCTTCGTTATCAACTCTGA

Coding sequence (CDS)

ATGGCCGAGATTGAACCACCATTAGAAGAAAGGTATAATGAGTTGAAGAAAGAGGAATTAAATGGCAGAAGAACTATAGAAGCTGATGAAGAAGTAATAGAAGAATGTGAAGAAGAAATAGAGTTACCAGTGATTGATTTAGGGCAACTAAAGAAGGGGAATTTAGAGAGAGAGAAATGCAAGAAAGAAATAGTGGAAGCAGCAATGAATTGGGGTTTTTTTCAAGTAATAAATCATGGGGTTGCTGAGAAAGTATTGAATGCAATGATAAATGAGCAAAAGAAAGTGTTTAATCAACCTTTTGTTAACAAAAGTTTGAGTACTAATTTTCTTAATTTGCCTTCAACTCACTACCGCTGGGGTAACCCTGTCGCTATTTCTTCCTCTCAAATCTCTTGGTCTGAAGCTTTTCATATTCCTGTTTCGGAAGTTTCAACCTCGCACCATCATATTACTCTCAGGTATGCCCTCTACCTTACTGTCCCTTCTTCACTTTCTCTTCGTTATCAACTCTGA

Homology

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BLAST of CSPI03G05430 vs. Swiss-Prot
Match: G2OX8_ARATH (Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana GN=GA2OX8 PE=1 SV=2)

HSP 1 Score: 117.1 bits (292), Expect = 1.9e-25
Identity = 73/177 (41.24%), Postives = 98/177 (55.37%), Query Frame = 1


Query: 4   IEPPLEERYNELKKEELNGRRTIEADEEVIE--------ECEEEIELPVIDLGQLKKG-N 63
           ++PP  E YN L   ++  +     D +V E           EE+ELPVID+ +L  G  
Sbjct: 1   MDPPFNEIYNNLLYNQITKK-----DNDVSEIPFSFSVTAVVEEVELPVIDVSRLIDGAE 60

Query: 64  LEREKCKKEIVEAAMNWGFFQVINHGVAEKVLNAMINEQKKVFNQPFVNKSLSTNFLNLP 123
            EREKCK+ I  A+  WGFFQVINHG++  VL  M  EQ +VF +PF  KS S  F    
Sbjct: 61  EEREKCKEAIARASREWGFFQVINHGISMDVLEKMRQEQIRVFREPFDKKSKSEKF---S 120

Query: 124 STHYRWGNPVAISSSQISWSEAFHIPVSEVSTSHHHITLRYALYLTVPSSLSLRYQL 172
           +  YRWG P A S  Q+SWSEAFH+P++++S +    TL   +      S +L Y L
Sbjct: 121 AGSYRWGTPSATSIRQLSWSEAFHVPMTDISDNKDFTTLSSTMEKFASESEALAYML 169

[Close]

BLAST of CSPI03G05430 vs. Swiss-Prot
Match: G2OX7_ARATH (Gibberellin 2-beta-dioxygenase 7 OS=Arabidopsis thaliana GN=GA2OX7 PE=1 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 1.2e-22
Identity = 55/107 (51.40%), Postives = 74/107 (69.16%), Query Frame = 1


Query: 40  IELPVIDLGQLKKGN-LEREKCKKEIVEAAMNWGFFQVINHGVAEKVLNAMINEQKKVFN 99
           I+LPVIDL  L  G  ++R++C K++V AA  WGFFQ++NHG+ + V   M+ E+KK+F+
Sbjct: 38  IKLPVIDLSHLTSGEEVKRKRCVKQMVAAAKEWGFFQIVNHGIPKDVFEMMLLEEKKLFD 97

Query: 100 QPFVNKSLSTNFLNLPSTHYRWGNPVAISSSQISWSEAFHIPVSEVS 146
           QPF  K +   F +L    YRWGNP A S +Q S SEAFHI +SEVS
Sbjct: 98  QPFSVK-VRERFSDLSKNSYRWGNPSATSPAQYSVSEAFHIILSEVS 143

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BLAST of CSPI03G05430 vs. Swiss-Prot
Match: ACCH3_ARATH (1-aminocyclopropane-1-carboxylate oxidase homolog 3 OS=Arabidopsis thaliana GN=At1g06650 PE=2 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 2.0e-06
Identity = 36/103 (34.95%), Postives = 51/103 (49.51%), Query Frame = 1


Query: 42  LPVIDLGQLK-KGNLEREKCKKEIVEAAMNWGFFQVINHGVAEKVLNAM------INEQK 101
           +P IDLG    +  ++R    +EI EAA  WGFFQVINHGV+ ++L  M       +EQ 
Sbjct: 64  IPTIDLGGRDFQDAIKRNNAIEEIKEAAAKWGFFQVINHGVSLELLEKMKKGVRDFHEQS 123

Query: 102 KVFNQPFVNKSLSTNFLNLPSTHYRWGNPVAISSSQISWSEAF 138
           +   + F ++  S  FL L        N    SS   +W + F
Sbjct: 124 QEVRKEFYSRDFSRRFLYL-------SNFDLFSSPAANWRDTF 159

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BLAST of CSPI03G05430 vs. Swiss-Prot
Match: NCS1_COPJA (S-norcoclaurine synthase 1 OS=Coptis japonica GN=NCS1 PE=1 SV=1)

HSP 1 Score: 53.5 bits (127), Expect = 2.6e-06
Identity = 37/124 (29.84%), Postives = 64/124 (51.61%), Query Frame = 1


Query: 27  EADEEVIEECEEEIELPVIDLGQLKKGNLEREKCKKEIVE---AAMNWGFFQVINHGVAE 86
           E + + +   +  +E+PVIDL +L    L+++    E+ +   A ++WGFFQ+INHGV E
Sbjct: 36  ELEHDDVVPIDNSLEIPVIDLSRL----LDQQYACDELAKFHSACLDWGFFQLINHGVRE 95

Query: 87  KVLNAMINEQKKVFNQPFVNKSLSTNFLNLPSTHYRWGNPVAISSSQ-ISWSEAFHI--- 144
           +V+  M  + +  F  PF  K+    +  LP+    +G     S  Q + W++   +   
Sbjct: 96  EVIEKMKVDTEDFFRLPFKEKNA---YRQLPNGMEGYGQAFVTSEEQKLDWADMHFLITK 152

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BLAST of CSPI03G05430 vs. Swiss-Prot
Match: F6H2_ARATH (Feruloyl CoA ortho-hydroxylase 2 OS=Arabidopsis thaliana GN=F6'H2 PE=1 SV=1)

HSP 1 Score: 52.8 bits (125), Expect = 4.5e-06
Identity = 35/85 (41.18%), Postives = 45/85 (52.94%), Query Frame = 1


Query: 32  VIEECEEEIELPVIDLGQLKKGNLEREKCKKEIVEAAMNWGFFQVINHGVAEKVLNAMIN 91
           V E+ +E I  PVID+      NL+ +   K + +AA  WGFFQVINHGV+ +VL  M  
Sbjct: 54  VKEDSDESI--PVIDIS-----NLDEKSVSKAVCDAAEEWGFFQVINHGVSMEVLENMKT 113

Query: 92  EQKKVFNQPFV-------NKSLSTN 110
              + F  P          KSLSTN
Sbjct: 114 ATHRFFGLPVEEKRKFSREKSLSTN 131

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BLAST of CSPI03G05430 vs. TrEMBL
Match: A0A0A0L322_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G081390 PE=4 SV=1)

HSP 1 Score: 335.5 bits (859), Expect = 3.8e-89
Identity = 170/171 (99.42%), Postives = 170/171 (99.42%), Query Frame = 1


Query: 1   MAEIEPPLEERYNELKKEELNGRRTIEADEEVIEECEEEIELPVIDLGQLKKGNLEREKC 60
           MAEIEPPLEERYNELKKEELNGRRTIEADEEVIEECEEEIELPVIDLGQLKKGNLEREKC
Sbjct: 1   MAEIEPPLEERYNELKKEELNGRRTIEADEEVIEECEEEIELPVIDLGQLKKGNLEREKC 60

Query: 61  KKEIVEAAMNWGFFQVINHGVAEKVLNAMINEQKKVFNQPFVNKSLSTNFLNLPSTHYRW 120
           KKEIVEAAMNWGFFQVINHGVAEKVLNAMINEQKKVFNQPFVNKSLSTNFLNLPSTHYRW
Sbjct: 61  KKEIVEAAMNWGFFQVINHGVAEKVLNAMINEQKKVFNQPFVNKSLSTNFLNLPSTHYRW 120

Query: 121 GNPVAISSSQISWSEAFHIPVSEVSTSHHHITLRYALYLTVPSSLSLRYQL 172
           GNPVAISSSQISWSEAFHIPV EVSTSHHHITLRYALYLTVPSSLSLRYQL
Sbjct: 121 GNPVAISSSQISWSEAFHIPVLEVSTSHHHITLRYALYLTVPSSLSLRYQL 171

[Close]

BLAST of CSPI03G05430 vs. TrEMBL
Match: B9H1H6_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0004s02200g PE=3 SV=2)

HSP 1 Score: 141.0 bits (354), Expect = 1.4e-30
Identity = 70/146 (47.95%), Postives = 94/146 (64.38%), Query Frame = 1


Query: 3   EIEPPLEERYNELKKEELNGRRTIEADEEVIEECEEEIELPVIDLGQLKKGNLEREKCKK 62
           +++PP +  Y  L ++   G    +   ++IEECE    LP+IDLG+L   NLE+EKCK 
Sbjct: 2   DVDPPFQLTYKTLLEKTTEGATDHKDVVDIIEECE----LPLIDLGRLNLKNLEKEKCKS 61

Query: 63  EIVEAAMNWGFFQVINHGVAEKVLNAMINEQKKVFNQPFVNKSLSTNFLNLPSTHYRWGN 122
           EI  A+  WGFFQV+NHG++ ++L+ M +EQ KVF QPF  KS    FLN     YRWG 
Sbjct: 62  EIARASREWGFFQVVNHGISREILDKMRSEQVKVFKQPFNEKSKEEKFLNFSRGTYRWGT 121

Query: 123 PVAISSSQISWSEAFHIPVSEVSTSH 149
           P A    Q+SWSEAFHIP+SE+  S+
Sbjct: 122 PTATCLKQLSWSEAFHIPMSEIQVSN 143

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BLAST of CSPI03G05430 vs. TrEMBL
Match: I1K1R2_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_05G081600 PE=3 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 1.2e-29
Identity = 73/155 (47.10%), Postives = 102/155 (65.81%), Query Frame = 1


Query: 3   EIEPPLEERYNELKKEELNGRRTIEADEEVIEECEEEIELPVIDLGQLKKGNLEREKCKK 62
           + EPP  E Y  L +  ++  +    D  ++E CE    LPVIDLG   K N ER++C+K
Sbjct: 2   DYEPPFLETYKALVQNHVDDSKN---DSSLVERCE----LPVIDLG---KFNYERDECEK 61

Query: 63  EIVEAAMNWGFFQVINHGVAEKVLNAMINEQKKVFNQPFVNKSLSTNFLNLPSTHYRWGN 122
           EI EAA  WGFFQV+NHG+++++L ++  EQKK+F QPFVNKS   NF +L +  YRWGN
Sbjct: 62  EIAEAANKWGFFQVVNHGISQELLKSLEFEQKKLFYQPFVNKSAKFNFSSLSAKTYRWGN 121

Query: 123 PVAISSSQISWSEAFHIPVSEVSTSHHHITLRYAL 158
           P A +  Q+SWSEAFH  +S++S    H ++R +L
Sbjct: 122 PFATNLRQLSWSEAFHFYLSDISWMDQHHSMRSSL 146

[Close]

BLAST of CSPI03G05430 vs. TrEMBL
Match: A0A0B2SGG6_GLYSO (Gibberellin 2-beta-dioxygenase 8 OS=Glycine soja GN=glysoja_047321 PE=3 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 1.2e-29
Identity = 73/155 (47.10%), Postives = 102/155 (65.81%), Query Frame = 1


Query: 3   EIEPPLEERYNELKKEELNGRRTIEADEEVIEECEEEIELPVIDLGQLKKGNLEREKCKK 62
           + EPP  E Y  L +  ++  +    D  ++E CE    LPVIDLG   K N ER++C+K
Sbjct: 2   DYEPPFLETYKALVQNHVDDSKN---DSSLVERCE----LPVIDLG---KFNYERDECEK 61

Query: 63  EIVEAAMNWGFFQVINHGVAEKVLNAMINEQKKVFNQPFVNKSLSTNFLNLPSTHYRWGN 122
           EI EAA  WGFFQV+NHG+++++L ++  EQKK+F QPFVNKS   NF +L +  YRWGN
Sbjct: 62  EIAEAANKWGFFQVVNHGISQELLKSLEFEQKKLFYQPFVNKSAKFNFSSLSAKTYRWGN 121

Query: 123 PVAISSSQISWSEAFHIPVSEVSTSHHHITLRYAL 158
           P A +  Q+SWSEAFH  +S++S    H ++R +L
Sbjct: 122 PFATNLRQLSWSEAFHFYLSDISWMDQHHSMRSSL 146

[Close]

BLAST of CSPI03G05430 vs. TrEMBL
Match: G7IJL9_MEDTR (Gibberellin 2-beta-dioxygenase OS=Medicago truncatula GN=MTR_2g083030 PE=3 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 1.2e-29
Identity = 71/155 (45.81%), Postives = 100/155 (64.52%), Query Frame = 1


Query: 3   EIEPPLEERYNELKKEELNGRRTIEADEEVIEECEEEIELPVIDLGQLKKGNLEREKCKK 62
           + EPP  + YN L ++ L       ++ ++  + E   ELP+IDL +L   + +RE+C K
Sbjct: 2   DFEPPFLKIYNTLLEKNLGDN----SENDLYSKVEGSEELPLIDLEKLNLEDPKREECMK 61

Query: 63  EIVEAAMNWGFFQVINHGVAEKVLNAMINEQKKVFNQPFVNKSLSTNFLNLPSTHYRWGN 122
           EI EAA  WGFFQ+INHG++ ++LN MI+EQKK+F QPFVNK  +    NL    YRWGN
Sbjct: 62  EISEAASKWGFFQIINHGISNEILNKMISEQKKLFYQPFVNKLSAETVFNLSPKTYRWGN 121

Query: 123 PVAISSSQISWSEAFHIPVSEVSTSHHHITLRYAL 158
           P A +  Q+SWSEAFH  ++++     HITLR +L
Sbjct: 122 PCATNLRQLSWSEAFHFALTDIPNMDQHITLRSSL 152

[Close]

BLAST of CSPI03G05430 vs. TAIR10
Match: AT4G21200.1 (AT4G21200.1 gibberellin 2-oxidase 8)

HSP 1 Score: 117.1 bits (292), Expect = 1.1e-26
Identity = 73/177 (41.24%), Postives = 98/177 (55.37%), Query Frame = 1


Query: 4   IEPPLEERYNELKKEELNGRRTIEADEEVIE--------ECEEEIELPVIDLGQLKKG-N 63
           ++PP  E YN L   ++  +     D +V E           EE+ELPVID+ +L  G  
Sbjct: 1   MDPPFNEIYNNLLYNQITKK-----DNDVSEIPFSFSVTAVVEEVELPVIDVSRLIDGAE 60

Query: 64  LEREKCKKEIVEAAMNWGFFQVINHGVAEKVLNAMINEQKKVFNQPFVNKSLSTNFLNLP 123
            EREKCK+ I  A+  WGFFQVINHG++  VL  M  EQ +VF +PF  KS S  F    
Sbjct: 61  EEREKCKEAIARASREWGFFQVINHGISMDVLEKMRQEQIRVFREPFDKKSKSEKF---S 120

Query: 124 STHYRWGNPVAISSSQISWSEAFHIPVSEVSTSHHHITLRYALYLTVPSSLSLRYQL 172
           +  YRWG P A S  Q+SWSEAFH+P++++S +    TL   +      S +L Y L
Sbjct: 121 AGSYRWGTPSATSIRQLSWSEAFHVPMTDISDNKDFTTLSSTMEKFASESEALAYML 169

[Close]

BLAST of CSPI03G05430 vs. TAIR10
Match: AT1G50960.1 (AT1G50960.1 gibberellin 2-oxidase 7)

HSP 1 Score: 107.8 bits (268), Expect = 6.6e-24
Identity = 55/107 (51.40%), Postives = 74/107 (69.16%), Query Frame = 1


Query: 40  IELPVIDLGQLKKGN-LEREKCKKEIVEAAMNWGFFQVINHGVAEKVLNAMINEQKKVFN 99
           I+LPVIDL  L  G  ++R++C K++V AA  WGFFQ++NHG+ + V   M+ E+KK+F+
Sbjct: 38  IKLPVIDLSHLTSGEEVKRKRCVKQMVAAAKEWGFFQIVNHGIPKDVFEMMLLEEKKLFD 97

Query: 100 QPFVNKSLSTNFLNLPSTHYRWGNPVAISSSQISWSEAFHIPVSEVS 146
           QPF  K +   F +L    YRWGNP A S +Q S SEAFHI +SEVS
Sbjct: 98  QPFSVK-VRERFSDLSKNSYRWGNPSATSPAQYSVSEAFHIILSEVS 143

[Close]

BLAST of CSPI03G05430 vs. TAIR10
Match: AT3G11180.2 (AT3G11180.2 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 65.5 bits (158), Expect = 3.7e-11
Identity = 37/102 (36.27%), Postives = 56/102 (54.90%), Query Frame = 1


Query: 39  EIELPVIDLGQLKKGNLEREKCKKEIVEAAMNWGFFQVINHGVAEKVLNAMINEQKKVFN 98
           +I +P+IDL  L  GN   E  KK I EA   WGFFQVINHGV  ++++A     K  FN
Sbjct: 92  DINIPIIDLDSLFSGN---EDDKKRISEACREWGFFQVINHGVKPELMDAARETWKSFFN 151

Query: 99  QPFVNKSLSTNFLNLPSTHYRWGNPVAISSSQI-SWSEAFHI 140
            P   K +   + N P T+  +G+ + +    I  W++ +++
Sbjct: 152 LPVEAKEV---YSNSPRTYEGYGSRLGVEKGAILDWNDYYYL 187

[Close]

BLAST of CSPI03G05430 vs. TAIR10
Match: AT1G06650.2 (AT1G06650.2 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 53.9 bits (128), Expect = 1.1e-07
Identity = 36/103 (34.95%), Postives = 51/103 (49.51%), Query Frame = 1


Query: 42  LPVIDLGQLK-KGNLEREKCKKEIVEAAMNWGFFQVINHGVAEKVLNAM------INEQK 101
           +P IDLG    +  ++R    +EI EAA  WGFFQVINHGV+ ++L  M       +EQ 
Sbjct: 64  IPTIDLGGRDFQDAIKRNNAIEEIKEAAAKWGFFQVINHGVSLELLEKMKKGVRDFHEQS 123

Query: 102 KVFNQPFVNKSLSTNFLNLPSTHYRWGNPVAISSSQISWSEAF 138
           +   + F ++  S  FL L        N    SS   +W + F
Sbjct: 124 QEVRKEFYSRDFSRRFLYL-------SNFDLFSSPAANWRDTF 159

[Close]

BLAST of CSPI03G05430 vs. TAIR10
Match: AT3G55970.1 (AT3G55970.1 jasmonate-regulated gene 21)

HSP 1 Score: 52.8 bits (125), Expect = 2.5e-07
Identity = 30/100 (30.00%), Postives = 56/100 (56.00%), Query Frame = 1


Query: 42  LPVIDLGQLKKGNLERE-KCKKEIVEAAMNWGFFQVINHGVAEKVLNAMINEQKKVFNQP 101
           +P+IDLG+L   +L  + K   EI +A    GFFQV+NHG++ ++++      ++ FN P
Sbjct: 52  IPIIDLGRLYTDDLTLQAKTLDEISKACRELGFFQVVNHGMSPQLMDQAKATWREFFNLP 111

Query: 102 FVNKSLSTNFLNLPSTHYRWGNPVAISSSQI-SWSEAFHI 140
              K++     N P T+  +G+ + +    I  WS+ +++
Sbjct: 112 MELKNMHA---NSPKTYEGYGSRLGVEKGAILDWSDYYYL 148

[Close]

BLAST of CSPI03G05430 vs. NCBI nr
Match: gi|700201030|gb|KGN56163.1| (hypothetical protein Csa_3G081390 [Cucumis sativus])

HSP 1 Score: 335.5 bits (859), Expect = 5.5e-89
Identity = 170/171 (99.42%), Postives = 170/171 (99.42%), Query Frame = 1


Query: 1   MAEIEPPLEERYNELKKEELNGRRTIEADEEVIEECEEEIELPVIDLGQLKKGNLEREKC 60
           MAEIEPPLEERYNELKKEELNGRRTIEADEEVIEECEEEIELPVIDLGQLKKGNLEREKC
Sbjct: 1   MAEIEPPLEERYNELKKEELNGRRTIEADEEVIEECEEEIELPVIDLGQLKKGNLEREKC 60

Query: 61  KKEIVEAAMNWGFFQVINHGVAEKVLNAMINEQKKVFNQPFVNKSLSTNFLNLPSTHYRW 120
           KKEIVEAAMNWGFFQVINHGVAEKVLNAMINEQKKVFNQPFVNKSLSTNFLNLPSTHYRW
Sbjct: 61  KKEIVEAAMNWGFFQVINHGVAEKVLNAMINEQKKVFNQPFVNKSLSTNFLNLPSTHYRW 120

Query: 121 GNPVAISSSQISWSEAFHIPVSEVSTSHHHITLRYALYLTVPSSLSLRYQL 172
           GNPVAISSSQISWSEAFHIPV EVSTSHHHITLRYALYLTVPSSLSLRYQL
Sbjct: 121 GNPVAISSSQISWSEAFHIPVLEVSTSHHHITLRYALYLTVPSSLSLRYQL 171

[Close]

BLAST of CSPI03G05430 vs. NCBI nr
Match: gi|778676167|ref|XP_004152933.2| (PREDICTED: gibberellin 2-beta-dioxygenase 8-like [Cucumis sativus])

HSP 1 Score: 304.7 bits (779), Expect = 1.0e-79
Identity = 153/154 (99.35%), Postives = 153/154 (99.35%), Query Frame = 1


Query: 1   MAEIEPPLEERYNELKKEELNGRRTIEADEEVIEECEEEIELPVIDLGQLKKGNLEREKC 60
           MAEIEPPLEERYNELKKEELNGRRTIEADEEVIEECEEEIELPVIDLGQLKKGNLEREKC
Sbjct: 1   MAEIEPPLEERYNELKKEELNGRRTIEADEEVIEECEEEIELPVIDLGQLKKGNLEREKC 60

Query: 61  KKEIVEAAMNWGFFQVINHGVAEKVLNAMINEQKKVFNQPFVNKSLSTNFLNLPSTHYRW 120
           KKEIVEAAMNWGFFQVINHGVAEKVLNAMINEQKKVFNQPFVNKSLSTNFLNLPSTHYRW
Sbjct: 61  KKEIVEAAMNWGFFQVINHGVAEKVLNAMINEQKKVFNQPFVNKSLSTNFLNLPSTHYRW 120

Query: 121 GNPVAISSSQISWSEAFHIPVSEVSTSHHHITLR 155
           GNPVAISSSQISWSEAFHIPV EVSTSHHHITLR
Sbjct: 121 GNPVAISSSQISWSEAFHIPVLEVSTSHHHITLR 154

[Close]

BLAST of CSPI03G05430 vs. NCBI nr
Match: gi|659126970|ref|XP_008463453.1| (PREDICTED: gibberellin 2-beta-dioxygenase 8-like [Cucumis melo])

HSP 1 Score: 287.7 bits (735), Expect = 1.3e-74
Identity = 145/152 (95.39%), Postives = 147/152 (96.71%), Query Frame = 1


Query: 3   EIEPPLEERYNELKKEELNGRRTIEADEEVIEECEEEIELPVIDLGQLKKGNLEREKCKK 62
           EIEPPLEERYNELKKEELNGRR IEADEE IEECEEEIELPVIDLGQLKKGNLERE+CKK
Sbjct: 2   EIEPPLEERYNELKKEELNGRRIIEADEEAIEECEEEIELPVIDLGQLKKGNLEREECKK 61

Query: 63  EIVEAAMNWGFFQVINHGVAEKVLNAMINEQKKVFNQPFVNKSLSTNFLNLPSTHYRWGN 122
           EIVEAAMNWGFFQVINHGVAEKVLNAMINEQKKVFNQPFVNKSLS NFLNLPSTHYRWGN
Sbjct: 62  EIVEAAMNWGFFQVINHGVAEKVLNAMINEQKKVFNQPFVNKSLSGNFLNLPSTHYRWGN 121

Query: 123 PVAISSSQISWSEAFHIPVSEVSTSHHHITLR 155
           PVAISSSQISWSEAFHIPV EVST +HHITLR
Sbjct: 122 PVAISSSQISWSEAFHIPVLEVSTLNHHITLR 153

[Close]

BLAST of CSPI03G05430 vs. NCBI nr
Match: gi|743926939|ref|XP_011007642.1| (PREDICTED: gibberellin 2-beta-dioxygenase 8-like [Populus euphratica])

HSP 1 Score: 146.4 bits (368), Expect = 4.7e-32
Identity = 72/146 (49.32%), Postives = 97/146 (66.44%), Query Frame = 1


Query: 3   EIEPPLEERYNELKKEELNGRRTIEADEEVIEECEEEIELPVIDLGQLKKGNLEREKCKK 62
           +++PP  + Y  L ++   G  T   D ++IEECE    LP+IDLG+L   NLE+EKC+ 
Sbjct: 2   DVDPPFPQTYKTLLEKTRKGA-TDHKDVDIIEECE----LPLIDLGRLNLKNLEKEKCET 61

Query: 63  EIVEAAMNWGFFQVINHGVAEKVLNAMINEQKKVFNQPFVNKSLSTNFLNLPSTHYRWGN 122
           EI  A+  WGFFQV+NHG++ ++L+ M +EQ KVF QPF+ KS   NFLN     YRWG 
Sbjct: 62  EIARASQEWGFFQVVNHGISREILDKMRSEQVKVFKQPFIEKSKEENFLNFSRGTYRWGT 121

Query: 123 PVAISSSQISWSEAFHIPVSEVSTSH 149
           P A    Q+SWSEAFHIP+SE+  S+
Sbjct: 122 PTATCLKQLSWSEAFHIPLSEIQVSN 142

[Close]

BLAST of CSPI03G05430 vs. NCBI nr
Match: gi|566164487|ref|XP_002304931.2| (hypothetical protein POPTR_0004s02200g [Populus trichocarpa])

HSP 1 Score: 141.0 bits (354), Expect = 2.0e-30
Identity = 70/146 (47.95%), Postives = 94/146 (64.38%), Query Frame = 1


Query: 3   EIEPPLEERYNELKKEELNGRRTIEADEEVIEECEEEIELPVIDLGQLKKGNLEREKCKK 62
           +++PP +  Y  L ++   G    +   ++IEECE    LP+IDLG+L   NLE+EKCK 
Sbjct: 2   DVDPPFQLTYKTLLEKTTEGATDHKDVVDIIEECE----LPLIDLGRLNLKNLEKEKCKS 61

Query: 63  EIVEAAMNWGFFQVINHGVAEKVLNAMINEQKKVFNQPFVNKSLSTNFLNLPSTHYRWGN 122
           EI  A+  WGFFQV+NHG++ ++L+ M +EQ KVF QPF  KS    FLN     YRWG 
Sbjct: 62  EIARASREWGFFQVVNHGISREILDKMRSEQVKVFKQPFNEKSKEEKFLNFSRGTYRWGT 121

Query: 123 PVAISSSQISWSEAFHIPVSEVSTSH 149
           P A    Q+SWSEAFHIP+SE+  S+
Sbjct: 122 PTATCLKQLSWSEAFHIPMSEIQVSN 143

The following BLAST results are available for this feature:

BLAST of CSPI03G05430 vs. Swiss-Prot


Match Name	E-value	Identity	Description
G2OX8_ARATH	1.9e-25	41.24	Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana GN=GA2OX8 PE=1 SV=2	[more]
G2OX7_ARATH	1.2e-22	51.40	Gibberellin 2-beta-dioxygenase 7 OS=Arabidopsis thaliana GN=GA2OX7 PE=1 SV=1	[more]
ACCH3_ARATH	2.0e-06	34.95	1-aminocyclopropane-1-carboxylate oxidase homolog 3 OS=Arabidopsis thaliana GN=A...	[more]
NCS1_COPJA	2.6e-06	29.84	S-norcoclaurine synthase 1 OS=Coptis japonica GN=NCS1 PE=1 SV=1	[more]
F6H2_ARATH	4.5e-06	41.18	Feruloyl CoA ortho-hydroxylase 2 OS=Arabidopsis thaliana GN=F6'H2 PE=1 SV=1	[more]

BLAST of CSPI03G05430 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L322_CUCSA	3.8e-89	99.42	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G081390 PE=4 SV=1	[more]
B9H1H6_POPTR	1.4e-30	47.95	Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0004s02200g PE=3 SV=2	[more]
I1K1R2_SOYBN	1.2e-29	47.10	Uncharacterized protein OS=Glycine max GN=GLYMA_05G081600 PE=3 SV=1	[more]
A0A0B2SGG6_GLYSO	1.2e-29	47.10	Gibberellin 2-beta-dioxygenase 8 OS=Glycine soja GN=glysoja_047321 PE=3 SV=1	[more]
G7IJL9_MEDTR	1.2e-29	45.81	Gibberellin 2-beta-dioxygenase OS=Medicago truncatula GN=MTR_2g083030 PE=3 SV=1	[more]

BLAST of CSPI03G05430 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G21200.1	1.1e-26	41.24	gibberellin 2-oxidase 8	[more]
AT1G50960.1	6.6e-24	51.40	gibberellin 2-oxidase 7	[more]
AT3G11180.2	3.7e-11	36.27	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot...	[more]
AT1G06650.2	1.1e-07	34.95	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot...	[more]
AT3G55970.1	2.5e-07	30.00	jasmonate-regulated gene 21	[more]

BLAST of CSPI03G05430 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700201030\|gb\|KGN56163.1\|	5.5e-89	99.42	hypothetical protein Csa_3G081390 [Cucumis sativus]	[more]
gi\|778676167\|ref\|XP_004152933.2\|	1.0e-79	99.35	PREDICTED: gibberellin 2-beta-dioxygenase 8-like [Cucumis sativus]	[more]
gi\|659126970\|ref\|XP_008463453.1\|	1.3e-74	95.39	PREDICTED: gibberellin 2-beta-dioxygenase 8-like [Cucumis melo]	[more]
gi\|743926939\|ref\|XP_011007642.1\|	4.7e-32	49.32	PREDICTED: gibberellin 2-beta-dioxygenase 8-like [Populus euphratica]	[more]
gi\|566164487\|ref\|XP_002304931.2\|	2.0e-30	47.95	hypothetical protein POPTR_0004s02200g [Populus trichocarpa]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR026992	DIOX_N
IPR027443	IPNS-like

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0071704	organic substance metabolic process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0016706	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0016491	oxidoreductase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G05430.1	CSPI03G05430.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 42..142 score: 3.1
IPR027443	Isopenicillin N synthase-like	GENE3D	G3DSA:2.60.120.330		coord: 34..144 score: 1.2
None	No IPR available	PANTHER	PTHR10209	OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEIN	coord: 27..147 score: 4.7
None	No IPR available	PANTHER	PTHR10209:SF90	GIBBERELLIN 2-BETA-DIOXYGENASE 7-RELATED	coord: 27..147 score: 4.7
None	No IPR available	unknown	SSF51197	Clavaminate synthase-like	coord: 38..145 score: 2.47

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CSPI03G05430	Cla005259	Watermelon (97103) v1	cpiwmB193
CSPI03G05430	Csa3G081390	Cucumber (Chinese Long) v2	cpicuB120
CSPI03G05430	ClCG10G005830	Watermelon (Charleston Gray)	cpiwcgB190
CSPI03G05430	Lsi05G006390	Bottle gourd (USVL1VR-Ls)	cpilsiB197
CSPI03G05430	CsaV3_3G005490	Cucumber (Chinese Long) v3	cpicucB144
CSPI03G05430	Cla97C10G190000	Watermelon (97103) v2	cpiwmbB184
CSPI03G05430	Bhi11G000533	Wax gourd	cpiwgoB234
CSPI03G05430	Cucsa.059620	Cucumber (Gy14) v1	cgycpiB044
CSPI03G05430	CmaCh04G009310	Cucurbita maxima (Rimu)	cmacpiB710
CSPI03G05430	CmaCh16G008420	Cucurbita maxima (Rimu)	cmacpiB334
CSPI03G05430	CmoCh16G009200	Cucurbita moschata (Rifu)	cmocpiB317
CSPI03G05430	CmoCh04G010010	Cucurbita moschata (Rifu)	cmocpiB703
CSPI03G05430	Cp4.1LG00g00200	Cucurbita pepo (Zucchini)	cpecpiB007
CSPI03G05430	Cp4.1LG01g08420	Cucurbita pepo (Zucchini)	cpecpiB392
CSPI03G05430	CsGy3G005550	Cucumber (Gy14) v2	cgybcpiB110
CSPI03G05430	Carg24829	Silver-seed gourd	carcpiB0828
CSPI03G05430	Carg00048	Silver-seed gourd	carcpiB0696

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CSPI03G05430	Cucurbita maxima (Rimu)	cmacpiB019
CSPI03G05430	Cucurbita moschata (Rifu)	cmocpiB010
CSPI03G05430	Melon (DHL92) v3.5.1	cpimeB231
CSPI03G05430	Melon (DHL92) v3.5.1	cpimeB242
CSPI03G05430	Cucurbita pepo (Zucchini)	cpecpiB783
CSPI03G05430	Melon (DHL92) v3.6.1	cpimedB226
CSPI03G05430	Melon (DHL92) v3.6.1	cpimedB228
CSPI03G05430	Silver-seed gourd	carcpiB0942
CSPI03G05430	Watermelon (97103) v2	cpiwmbB217
CSPI03G05430	Wax gourd	cpiwgoB218