CmoCh01G010040 (gene) Cucurbita moschata (Rifu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmoCh01G010040
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu))
Description	(Ribosome inactivating protein) (3.2.2.22)
Location	Cmo_Chr01 : 7159573 .. 7160490 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGTGCATTGGATCAACCCAAGACTTGATACAAGCTCCCTTTCCCTTAAACTGTGGTGCTCATGCTGCCATTCGTGCTCCTCGTCCTTCCGCCAATGCTAATCTCAGCTTCTCTCTGTTGGGTGCCACTGCCCAAACCTACAAACAATTCATTAATGATCTTCGTAAGGCACTTACGGTAAACTCCCACAAAGTGTACGACATTCCTGTGCTGCCAGCCACAGCCAAAGGGTTAGCACGCTTCATATTGGTCAATCTCACCAATTACAAAGGTGAATCCATCACTGTTGCAATCGACACAGTGGATGTCTATATTGTCGCATACCAAGCAGGAAACAAGGCTTATTTTCTAAAAGATGTTTCCAAGGAAGCCCATGATGTGCTATTTAAAGGTAGCAAACATGAAACGCTGTCGTATAAAGGGAATTATGATGATCTTGAGAGGCATGCTGGTAAGATTTCAAGAGAGAATATTGAGCTTGGATTCTCTGAATTAAGCAGCTCCATTGGCAACATGTTCCACTATAAGGCTGGCTATTCTGTGCCTAGATCGTTTATTGTGATGATTCAAATGCTTTCAGAAGCTTCGAGGTTTAAGTATATTGAGACGAGAAATTGTGAGAAGGTTGAATCAAAGTTTAAGCCAGATCCTGGATACTTGAGTTTAGAGAACCGTTGGTCTAATTTATCTGAACAGGTACAGAAGGCGCAAAAGCATGGAGGTAAATTTGAGCATCCAGTTCAAGTTCGAAGTGTTAGCAATAAGCCAGTTCTGGTGAGTGATGTTAATTCCCCTGTTGTTAAAGGAATAGCGCTGTTACTTTCCAACAAGCCAGGGGTTAATGGAGTATCCAAATTGATGGAAGAGTTTATCCATTGGGAAGCTGCTCGAGGTCATTTTGGAATTGATTTCTGA

mRNA sequence

ATGGAGTGCATTGGATCAACCCAAGACTTGATACAAGCTCCCTTTCCCTTAAACTGTGGTGCTCATGCTGCCATTCGTGCTCCTCGTCCTTCCGCCAATGCTAATCTCAGCTTCTCTCTGTTGGGTGCCACTGCCCAAACCTACAAACAATTCATTAATGATCTTCGTAAGGCACTTACGGTAAACTCCCACAAAGTGTACGACATTCCTGTGCTGCCAGCCACAGCCAAAGGGTTAGCACGCTTCATATTGGTCAATCTCACCAATTACAAAGGTGAATCCATCACTGTTGCAATCGACACAGTGGATGTCTATATTGTCGCATACCAAGCAGGAAACAAGGCTTATTTTCTAAAAGATGTTTCCAAGGAAGCCCATGATGTGCTATTTAAAGGTAGCAAACATGAAACGCTGTCGTATAAAGGGAATTATGATGATCTTGAGAGGCATGCTGGTAAGATTTCAAGAGAGAATATTGAGCTTGGATTCTCTGAATTAAGCAGCTCCATTGGCAACATGTTCCACTATAAGGCTGGCTATTCTGTGCCTAGATCGTTTATTGTGATGATTCAAATGCTTTCAGAAGCTTCGAGGTTTAAGTATATTGAGACGAGAAATTGTGAGAAGGTTGAATCAAAGTTTAAGCCAGATCCTGGATACTTGAGTTTAGAGAACCGTTGGTCTAATTTATCTGAACAGGTACAGAAGGCGCAAAAGCATGGAGGTAAATTTGAGCATCCAGTTCAAGTTCGAAGTGTTAGCAATAAGCCAGTTCTGGTGAGTGATGTTAATTCCCCTGTTGTTAAAGGAATAGCGCTGTTACTTTCCAACAAGCCAGGGGTTAATGGAGTATCCAAATTGATGGAAGAGTTTATCCATTGGGAAGCTGCTCGAGGTCATTTTGGAATTGATTTCTGA

Coding sequence (CDS)

ATGGAGTGCATTGGATCAACCCAAGACTTGATACAAGCTCCCTTTCCCTTAAACTGTGGTGCTCATGCTGCCATTCGTGCTCCTCGTCCTTCCGCCAATGCTAATCTCAGCTTCTCTCTGTTGGGTGCCACTGCCCAAACCTACAAACAATTCATTAATGATCTTCGTAAGGCACTTACGGTAAACTCCCACAAAGTGTACGACATTCCTGTGCTGCCAGCCACAGCCAAAGGGTTAGCACGCTTCATATTGGTCAATCTCACCAATTACAAAGGTGAATCCATCACTGTTGCAATCGACACAGTGGATGTCTATATTGTCGCATACCAAGCAGGAAACAAGGCTTATTTTCTAAAAGATGTTTCCAAGGAAGCCCATGATGTGCTATTTAAAGGTAGCAAACATGAAACGCTGTCGTATAAAGGGAATTATGATGATCTTGAGAGGCATGCTGGTAAGATTTCAAGAGAGAATATTGAGCTTGGATTCTCTGAATTAAGCAGCTCCATTGGCAACATGTTCCACTATAAGGCTGGCTATTCTGTGCCTAGATCGTTTATTGTGATGATTCAAATGCTTTCAGAAGCTTCGAGGTTTAAGTATATTGAGACGAGAAATTGTGAGAAGGTTGAATCAAAGTTTAAGCCAGATCCTGGATACTTGAGTTTAGAGAACCGTTGGTCTAATTTATCTGAACAGGTACAGAAGGCGCAAAAGCATGGAGGTAAATTTGAGCATCCAGTTCAAGTTCGAAGTGTTAGCAATAAGCCAGTTCTGGTGAGTGATGTTAATTCCCCTGTTGTTAAAGGAATAGCGCTGTTACTTTCCAACAAGCCAGGGGTTAATGGAGTATCCAAATTGATGGAAGAGTTTATCCATTGGGAAGCTGCTCGAGGTCATTTTGGAATTGATTTCTGA

Homology

[Close]

BLAST of CmoCh01G010040 vs. Swiss-Prot
Match: RIP2_BRYDI (Ribosome-inactivating protein bryodin II OS=Bryonia dioica PE=1 SV=1)

HSP 1 Score: 308.1 bits (788), Expect = 1.0e-82
Identity = 161/259 (62.16%), Postives = 196/259 (75.68%), Query Frame = 1


Query: 17  LNCGAHAAIRAPRPSANANLSFSLLGATAQTYKQFINDLRKALTVNSHKVYDIPVLPATA 76
           L  GAH        + + +++FSL+GAT  TYK FI +LR  LTV + +VYDIPVL   A
Sbjct: 12  LYVGAHV-------TEDVDINFSLIGATGATYKTFIRNLRTKLTVGTPRVYDIPVLRNAA 71

Query: 77  KGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYFLKDVSKEAHDVLFKGSKHE 136
            GLARF LV LTNY GES+TVA+D V+VY+VAY+AGN AYFL D S EA++VLF G  H 
Sbjct: 72  AGLARFQLVTLTNYNGESVTVALDVVNVYVVAYRAGNTAYFLADASTEANNVLFAGINHV 131

Query: 137 TLSYKGNYDDLERHAGKISRENIELGFSELSSSIGNMFHYKAGYSVPRSFIVMIQMLSEA 196
            L Y GNYD LE  AG+ISRENIELGFSE+SS+IGNMF +  G SVPR+FIV+IQ +SEA
Sbjct: 132 RLPYGGNYDGLETAAGRISRENIELGFSEISSAIGNMFRHNPGTSVPRAFIVIIQTVSEA 191

Query: 197 SRFKYIETRNCEKVESKFKPDPGYLSLENRWSNLSEQVQKAQKHGGKFEHPVQVRSVSNK 256
           +RFKYIE R  E V +KFKPDP +LSL+N W +LSEQ+Q AQ  GG+F  PV++R+VSN 
Sbjct: 192 ARFKYIEQRVSENVGTKFKPDPAFLSLQNAWGSLSEQIQIAQTRGGEFARPVELRTVSNT 251

Query: 257 PVLVSDVNSPVVKGIALLL 276
           P  V++VNSPVVKGIALLL
Sbjct: 252 PTFVTNVNSPVVKGIALLL 263

[Close]

BLAST of CmoCh01G010040 vs. Swiss-Prot
Match: RIP3_MOMCH (Ribosome-inactivating protein beta-momorcharin OS=Momordica charantia GN=MAP30 PE=1 SV=2)

HSP 1 Score: 213.4 bits (542), Expect = 3.5e-54
Identity = 120/250 (48.00%), Postives = 172/250 (68.80%), Query Frame = 1


Query: 30  PSANANLSFSLLGATAQTYKQFINDLRKALTVNSHKVYDIPVLPATAKGLARFILVNLTN 89
           P+A  +++F L  ATA+TY +FI D R  L   SHKVYDIP+L +T     RFIL+NLT+
Sbjct: 19  PTAKGDVNFDLSTATAKTYTKFIEDFRATLPF-SHKVYDIPLLYSTISDSRRFILLNLTS 78

Query: 90  YKGESITVAIDTVDVYIVAYQAGNKAYFLKDVSKEAHDVLFKGSKHETLSYKGNYDDLER 149
           Y  E+I+VAID  +VY+VAY+  + +YF K+   EA+++LFKG++  TL Y GNY++L+ 
Sbjct: 79  YAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKGTRKITLPYTGNYENLQT 138

Query: 150 HAGKISRENIELGFSELSSSIGNMFHYKAGYSVPRSFIVMIQMLSEASRFKYIETRNCEK 209
            A KI RENI+LG   LSS+I  +F+Y A  S P + +V+IQ  +EA+RFKYIE    + 
Sbjct: 139 AAHKI-RENIDLGLPALSSAITTLFYYNA-QSAPSALLVLIQTTAEAARFKYIERHVAKY 198

Query: 210 VESKFKPDPGYLSLENRWSNLSEQVQKAQKHGGKFEHPVQVRSVSNKPVLVSDVNSPVVK 269
           V + FKP+   +SLEN+WS LS+Q+  AQ  GGKF +PV +   + +   V++V+S VVK
Sbjct: 199 VATNFKPNLAIISLENQWSALSKQIFLAQNQGGKFRNPVDLIKPTGERFQVTNVDSDVVK 258

Query: 270 G-IALLLSNK 279
           G I LLL+++
Sbjct: 259 GNIKLLLNSR 265

[Close]

BLAST of CmoCh01G010040 vs. Swiss-Prot
Match: RIP2_MOMBA (Ribosome-inactivating protein momordin II OS=Momordica balsamina PE=3 SV=1)

HSP 1 Score: 211.5 bits (537), Expect = 1.3e-53
Identity = 119/250 (47.60%), Postives = 172/250 (68.80%), Query Frame = 1


Query: 30  PSANANLSFSLLGATAQTYKQFINDLRKALTVNSHKVYDIPVLPATAKGLARFILVNLTN 89
           P+A  +++F L  ATA+TY +FI D R  L   SHKVYDIP+L +T     RFIL++LT+
Sbjct: 19  PTAKGDVNFDLSTATAKTYTKFIEDFRATLPF-SHKVYDIPLLYSTISDSRRFILLDLTS 78

Query: 90  YKGESITVAIDTVDVYIVAYQAGNKAYFLKDVSKEAHDVLFKGSKHETLSYKGNYDDLER 149
           Y  E+I+VAID  +VY+VAY+  + +YF K+   EA+++LFKG++  TL Y GNY++L+ 
Sbjct: 79  YAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKGTRKITLPYTGNYENLQT 138

Query: 150 HAGKISRENIELGFSELSSSIGNMFHYKAGYSVPRSFIVMIQMLSEASRFKYIETRNCEK 209
            A KI RENI+LG   LSS+I  +F+Y A  S P + +V+IQ  +EA+RFKYIE    + 
Sbjct: 139 AAHKI-RENIDLGLPALSSAITTLFYYNA-QSAPSALLVLIQTTAEAARFKYIERHVAKY 198

Query: 210 VESKFKPDPGYLSLENRWSNLSEQVQKAQKHGGKFEHPVQVRSVSNKPVLVSDVNSPVVK 269
           V + FKP+   +SLEN+WS LS+Q+  AQ  GGKF +PV +   + +   V++V+S VVK
Sbjct: 199 VATNFKPNLAIISLENQWSALSKQIFLAQNQGGKFRNPVDLIKPTGERFQVTNVDSDVVK 258

Query: 270 G-IALLLSNK 279
           G I LLL+++
Sbjct: 259 GNIKLLLNSR 265

[Close]

BLAST of CmoCh01G010040 vs. Swiss-Prot
Match: RIP_CUCMO (Ribosome-inactivating protein cucurmosin OS=Cucurbita moschata PE=1 SV=2)

HSP 1 Score: 199.9 bits (507), Expect = 4.0e-50
Identity = 115/243 (47.33%), Postives = 158/243 (65.02%), Query Frame = 1


Query: 35  NLSFSLLGATAQTYKQFINDLRKALTVNSHKVYDIPVLPATAKGLARFILVNLTNYKGES 94
           N+ F L  AT+ +YK FI +LR+AL  +  KVYDIPVL +T     RFIL++L NY G+S
Sbjct: 1   NVRFDLSSATSSSYKTFIKNLREALPKDG-KVYDIPVLLSTVMDSRRFILIDLVNYDGQS 60

Query: 95  ITVAIDTVDVYIVAYQAGNKAYFLKDVSKEAHDVLFKGSKHETLSYKGNYDDLERHAGKI 154
           IT AID ++VYIVAY  G  +YF + V  +A  +LFKG++  TL Y GNY++L+  A K+
Sbjct: 61  ITAAIDVLNVYIVAYSTGTVSYFFQQVPAQAPKLLFKGTQQRTLPYTGNYENLQTAAKKL 120

Query: 155 SRENIELGFSELSSSIGNMFHYKAGYSVPRSFIVMIQMLSEASRFKYIETRNCEKVESKF 214
            RENIELG   L S+I  +FHY A  +   + +V+IQ  SEA+RF+YIE +    V +KF
Sbjct: 121 -RENIELGLPALDSAITTLFHYNA-EAAASALLVLIQTTSEAARFRYIELQIANNVGTKF 180

Query: 215 KPDPGYLSLENRWSNLSEQVQKAQKHGGKFEHPVQVRSVSNKPVLVSDVNSPVV-KGIAL 274
           KP    +SLEN WS LS+Q+Q A+   G+FE PV +       V +++V S VV + I L
Sbjct: 181 KPSQTIISLENNWSALSKQIQIAKNKNGQFETPVILIDPQGNRVQITNVTSNVVTQNIQL 240

Query: 275 LLS 277
           LL+
Sbjct: 241 LLN 240

[Close]

BLAST of CmoCh01G010040 vs. Swiss-Prot
Match: RIPT_TRIKI (Ribosome-inactivating protein alpha-trichosanthin OS=Trichosanthes kirilowii PE=1 SV=2)

HSP 1 Score: 188.7 bits (478), Expect = 9.2e-47
Identity = 111/250 (44.40%), Postives = 165/250 (66.00%), Query Frame = 1


Query: 30  PSANANLSFSLLGATAQTYKQFINDLRKALTVNSHKVYDIPVLPATAKGLARFILVNLTN 89
           P+   ++SF L GAT+ +Y  FI++LRKAL  N  K+YDIP+L ++  G  R+ L++LTN
Sbjct: 19  PAVEGDVSFRLSGATSSSYGVFISNLRKALP-NERKLYDIPLLRSSLPGSQRYALIHLTN 78

Query: 90  YKGESITVAIDTVDVYIVAYQAGNKAYFLKDVSK-EAHDVLFKGSKHE-TLSYKGNYDDL 149
           Y  E+I+VAID  +VYI+ Y+AG+ +YF  + S  EA   +FK +  + TL Y GNY+ L
Sbjct: 79  YADETISVAIDVTNVYIMGYRAGDTSYFFNEASATEAAKYVFKDAMRKVTLPYSGNYERL 138

Query: 150 ERHAGKISRENIELGFSELSSSIGNMFHYKAGYSVPRSFIVMIQMLSEASRFKYIETRNC 209
           +  AGKI RENI LG   L S+I  +F+Y A  S   + +V+IQ  SEA+R+K+IE +  
Sbjct: 139 QTAAGKI-RENIPLGLPALDSAITTLFYYNAN-SAASALMVLIQSTSEAARYKFIEQQIG 198

Query: 210 EKVESKFKPDPGYLSLENRWSNLSEQVQKAQKHGGKFEHPVQVRSVSNKPVLVSDVNSPV 269
           ++V+  F P    +SLEN WS LS+Q+Q A  + G+FE PV + +  N+ V +++V++ V
Sbjct: 199 KRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQFESPVVLINAQNQRVTITNVDAGV 258

Query: 270 V-KGIALLLS 277
           V   IALLL+
Sbjct: 259 VTSNIALLLN 265

[Close]

BLAST of CmoCh01G010040 vs. TrEMBL
Match: B1B626_TRIKI (rRNA N-glycosidase OS=Trichosanthes kirilowii var. japonica PE=2 SV=1)

HSP 1 Score: 314.7 bits (805), Expect = 1.2e-82
Identity = 160/248 (64.52%), Postives = 197/248 (79.44%), Query Frame = 1


Query: 34  ANLSFSLLGATAQTYKQFINDLRKALTVNSHKVYDIPVLPATAKGLARFILVNLTNYKGE 93
           A+++FSLLG+T+ TYKQFI +LR ALTV S  V +IPVL  TA GLARF+LV+LTNY GE
Sbjct: 21  ADVNFSLLGSTSATYKQFIQNLRAALTVGSPTVENIPVLKGTAAGLARFLLVHLTNYNGE 80

Query: 94  SITVAIDTVDVYIVAYQAGNKAYFLKDVSKEAHDVLFKGSKHETLSYKGNYDDLERHAGK 153
           SITVA+D V+VY+VAY+AGN AYFL D S EA++VLFKG  H  L Y GNYD LE  AG+
Sbjct: 81  SITVAVDVVNVYVVAYRAGNNAYFLNDASAEANNVLFKGINHVRLPYGGNYDGLETAAGR 140

Query: 154 ISRENIELGFSELSSSIGNMFHYKAGYSVPRSFIVMIQMLSEASRFKYIETRNCEKVESK 213
           ISRE I LGFSE+SS+IG+MFHY AG SVP++FIV+IQ +SEA+RFKYI+ R  E V++K
Sbjct: 141 ISREKIYLGFSEISSAIGSMFHYNAGTSVPKAFIVIIQTVSEAARFKYIQQRVSENVQTK 200

Query: 214 FKPDPGYLSLENRWSNLSEQVQKAQKHGGKFEHPVQVRSVSNKPVLVSDVNSPVVKGIAL 273
           F PDP +LSLEN W  LSEQ+Q AQ  GG+F  P+++R+VSN P  V++VNSPVVKG+AL
Sbjct: 201 FLPDPAFLSLENAWGGLSEQIQIAQYRGGEFARPIELRTVSNTPTFVTNVNSPVVKGVAL 260

Query: 274 LLSNKPGV 282
           LL  K  V
Sbjct: 261 LLYYKVNV 268

[Close]

BLAST of CmoCh01G010040 vs. TrEMBL
Match: S6CNA0_MOMCH (Ribosome inactivating protein (Fragment) OS=Momordica charantia GN=rip PE=4 SV=1)

HSP 1 Score: 216.1 bits (549), Expect = 6.0e-53
Identity = 121/250 (48.40%), Postives = 173/250 (69.20%), Query Frame = 1


Query: 30  PSANANLSFSLLGATAQTYKQFINDLRKALTVNSHKVYDIPVLPATAKGLARFILVNLTN 89
           P+A  +++F L  ATA+TY +FI D R  L   SHKVYDIP+L +T  G  RFIL+NLT+
Sbjct: 13  PTAKGDVNFDLSTATAKTYTKFIEDFRATLPF-SHKVYDIPLLYSTISGSRRFILLNLTS 72

Query: 90  YKGESITVAIDTVDVYIVAYQAGNKAYFLKDVSKEAHDVLFKGSKHETLSYKGNYDDLER 149
           Y  E+I+VAID  +VY+VAY+  + +YF K+   EA+++LFKG++  TL Y GNY++L+ 
Sbjct: 73  YAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKGTRKITLPYTGNYENLQT 132

Query: 150 HAGKISRENIELGFSELSSSIGNMFHYKAGYSVPRSFIVMIQMLSEASRFKYIETRNCEK 209
            A KI RENI+LG   LSS+I  +F+Y A  S P + +V+IQ  +EA+RFKYIE    + 
Sbjct: 133 AAHKI-RENIDLGLPALSSAITTLFYYNA-QSAPSALLVLIQTTAEAARFKYIERHVAKY 192

Query: 210 VESKFKPDPGYLSLENRWSNLSEQVQKAQKHGGKFEHPVQVRSVSNKPVLVSDVNSPVVK 269
           V + FKP+   +SLEN+WS LS+Q+  AQ  GGKF +PV +   + +   V++V+S VVK
Sbjct: 193 VATNFKPNLAIISLENQWSALSKQIFLAQNQGGKFRNPVDLIKPTGERFQVTNVDSDVVK 252

Query: 270 G-IALLLSNK 279
           G I LLL+++
Sbjct: 253 GNIKLLLNSR 259

[Close]

BLAST of CmoCh01G010040 vs. TrEMBL
Match: S6CNH6_MOMCH (Ribosome inactivating protein (Fragment) OS=Momordica charantia GN=rip PE=4 SV=1)

HSP 1 Score: 215.7 bits (548), Expect = 7.8e-53
Identity = 121/250 (48.40%), Postives = 173/250 (69.20%), Query Frame = 1


Query: 30  PSANANLSFSLLGATAQTYKQFINDLRKALTVNSHKVYDIPVLPATAKGLARFILVNLTN 89
           P+A  +++F L  ATA+TY +FI D R  L   SHKVYDIP+L +T     RFIL+NLT+
Sbjct: 13  PTAKGDVNFDLSTATAKTYTKFIEDFRATLPF-SHKVYDIPLLYSTISDSRRFILLNLTS 72

Query: 90  YKGESITVAIDTVDVYIVAYQAGNKAYFLKDVSKEAHDVLFKGSKHETLSYKGNYDDLER 149
           Y  E+I+VAID  +VY+VAY+  + +YF K+   EA+++LFKG++  TL Y GNY++L+ 
Sbjct: 73  YAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKGTRKITLPYTGNYENLQT 132

Query: 150 HAGKISRENIELGFSELSSSIGNMFHYKAGYSVPRSFIVMIQMLSEASRFKYIETRNCEK 209
            A KI RENI+LGF  LSS+I  +F+Y A  S P + +V+IQ  +EA+RFKYIE    + 
Sbjct: 133 AAHKI-RENIDLGFPALSSAITTLFYYNA-QSAPSALLVLIQTTAEAARFKYIERHVAKY 192

Query: 210 VESKFKPDPGYLSLENRWSNLSEQVQKAQKHGGKFEHPVQVRSVSNKPVLVSDVNSPVVK 269
           V + FKP+   +SLEN+WS LS+Q+  AQ  GGKF +PV +   + +   V++V+S VVK
Sbjct: 193 VATNFKPNLAIISLENQWSALSKQIFLAQNQGGKFRNPVDLIKPTGERFQVTNVDSDVVK 252

Query: 270 G-IALLLSNK 279
           G I LLL+++
Sbjct: 253 GNIKLLLNSR 259

[Close]

BLAST of CmoCh01G010040 vs. TrEMBL
Match: Q5PZ05_MOMCH (rRNA N-glycosidase OS=Momordica charantia PE=3 SV=1)

HSP 1 Score: 213.4 bits (542), Expect = 3.9e-52
Identity = 120/250 (48.00%), Postives = 172/250 (68.80%), Query Frame = 1


Query: 30  PSANANLSFSLLGATAQTYKQFINDLRKALTVNSHKVYDIPVLPATAKGLARFILVNLTN 89
           P+A  +++F L  ATA+TY +FI D R  L   SHKVYDIP+L +T     RFIL+NLT+
Sbjct: 19  PTAKGDVNFDLSTATAKTYTKFIEDFRATLPF-SHKVYDIPLLYSTISDSRRFILLNLTS 78

Query: 90  YKGESITVAIDTVDVYIVAYQAGNKAYFLKDVSKEAHDVLFKGSKHETLSYKGNYDDLER 149
           Y  E+I+VAID  +VY+VAY+  + +YF K+   EA+++LFKG++  TL Y GNY++L+ 
Sbjct: 79  YAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKGTRKITLPYTGNYENLQT 138

Query: 150 HAGKISRENIELGFSELSSSIGNMFHYKAGYSVPRSFIVMIQMLSEASRFKYIETRNCEK 209
            A KI RENI+LG   LSS+I  +F+Y A  S P + +V+IQ  +EA+RFKYIE    + 
Sbjct: 139 AAHKI-RENIDLGLPALSSAITTLFYYNA-QSAPSALLVLIQTTAEAARFKYIERHVAKY 198

Query: 210 VESKFKPDPGYLSLENRWSNLSEQVQKAQKHGGKFEHPVQVRSVSNKPVLVSDVNSPVVK 269
           V + FKP+   +SLEN+WS LS+Q+  AQ  GGKF +PV +   + +   V++V+S VVK
Sbjct: 199 VATNFKPNLAIISLENQWSALSKQIFLAQNQGGKFRNPVDLIKPTGERFQVTNVDSDVVK 258

Query: 270 G-IALLLSNK 279
           G I LLL+++
Sbjct: 259 GNIKLLLNSR 265

[Close]

BLAST of CmoCh01G010040 vs. TrEMBL
Match: S6CMM2_MOMCH (Ribosome inactivating protein (Fragment) OS=Momordica charantia GN=rip PE=4 SV=1)

HSP 1 Score: 213.4 bits (542), Expect = 3.9e-52
Identity = 120/250 (48.00%), Postives = 172/250 (68.80%), Query Frame = 1


Query: 30  PSANANLSFSLLGATAQTYKQFINDLRKALTVNSHKVYDIPVLPATAKGLARFILVNLTN 89
           P+A  +++F L  ATA+TY +FI D R  L   SHKVYDIP+L +T     RFIL+NLT+
Sbjct: 13  PAAKGDVNFDLSTATAKTYTKFIEDFRATLPF-SHKVYDIPLLYSTISDSRRFILLNLTS 72

Query: 90  YKGESITVAIDTVDVYIVAYQAGNKAYFLKDVSKEAHDVLFKGSKHETLSYKGNYDDLER 149
           Y  E+I+VAID  +VY+VAY+  + +YF K+   EA+++LFKG++  TL Y GNY++L+ 
Sbjct: 73  YAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKGTRKITLPYTGNYENLQT 132

Query: 150 HAGKISRENIELGFSELSSSIGNMFHYKAGYSVPRSFIVMIQMLSEASRFKYIETRNCEK 209
            A KI RENI+LG   LSS+I  +F+Y A  S P + +V+IQ  +EA+RFKYIE    + 
Sbjct: 133 AAHKI-RENIDLGLPALSSAITTLFYYNA-QSAPSALLVLIQTTAEAARFKYIERHVAKY 192

Query: 210 VESKFKPDPGYLSLENRWSNLSEQVQKAQKHGGKFEHPVQVRSVSNKPVLVSDVNSPVVK 269
           V + FKP+   +SLEN+WS LS+Q+  AQ  GGKF +PV +   + +   V++V+S VVK
Sbjct: 193 VATNFKPNLAIISLENQWSALSKQIFLAQNQGGKFRNPVDLIKPTGERFQVTNVDSDVVK 252

Query: 270 G-IALLLSNK 279
           G I LLL+++
Sbjct: 253 GNIKLLLNSR 259

[Close]

BLAST of CmoCh01G010040 vs. NCBI nr
Match: gi|169635959|dbj|BAG12408.1| (karasurin-H precursor [Trichosanthes kirilowii var. japonica])

HSP 1 Score: 314.7 bits (805), Expect = 1.8e-82
Identity = 160/248 (64.52%), Postives = 197/248 (79.44%), Query Frame = 1


Query: 34  ANLSFSLLGATAQTYKQFINDLRKALTVNSHKVYDIPVLPATAKGLARFILVNLTNYKGE 93
           A+++FSLLG+T+ TYKQFI +LR ALTV S  V +IPVL  TA GLARF+LV+LTNY GE
Sbjct: 21  ADVNFSLLGSTSATYKQFIQNLRAALTVGSPTVENIPVLKGTAAGLARFLLVHLTNYNGE 80

Query: 94  SITVAIDTVDVYIVAYQAGNKAYFLKDVSKEAHDVLFKGSKHETLSYKGNYDDLERHAGK 153
           SITVA+D V+VY+VAY+AGN AYFL D S EA++VLFKG  H  L Y GNYD LE  AG+
Sbjct: 81  SITVAVDVVNVYVVAYRAGNNAYFLNDASAEANNVLFKGINHVRLPYGGNYDGLETAAGR 140

Query: 154 ISRENIELGFSELSSSIGNMFHYKAGYSVPRSFIVMIQMLSEASRFKYIETRNCEKVESK 213
           ISRE I LGFSE+SS+IG+MFHY AG SVP++FIV+IQ +SEA+RFKYI+ R  E V++K
Sbjct: 141 ISREKIYLGFSEISSAIGSMFHYNAGTSVPKAFIVIIQTVSEAARFKYIQQRVSENVQTK 200

Query: 214 FKPDPGYLSLENRWSNLSEQVQKAQKHGGKFEHPVQVRSVSNKPVLVSDVNSPVVKGIAL 273
           F PDP +LSLEN W  LSEQ+Q AQ  GG+F  P+++R+VSN P  V++VNSPVVKG+AL
Sbjct: 201 FLPDPAFLSLENAWGGLSEQIQIAQYRGGEFARPIELRTVSNTPTFVTNVNSPVVKGVAL 260

Query: 274 LLSNKPGV 282
           LL  K  V
Sbjct: 261 LLYYKVNV 268

[Close]

BLAST of CmoCh01G010040 vs. NCBI nr
Match: gi|12643707|sp|P98184.1|RIP2_BRYDI (RecName: Full=Ribosome-inactivating protein bryodin II; AltName: Full=BD2; AltName: Full=rRNA N-glycosidase; Flags: Precursor [Bryonia dioica])

HSP 1 Score: 308.1 bits (788), Expect = 1.7e-80
Identity = 161/259 (62.16%), Postives = 196/259 (75.68%), Query Frame = 1


Query: 17  LNCGAHAAIRAPRPSANANLSFSLLGATAQTYKQFINDLRKALTVNSHKVYDIPVLPATA 76
           L  GAH        + + +++FSL+GAT  TYK FI +LR  LTV + +VYDIPVL   A
Sbjct: 12  LYVGAHV-------TEDVDINFSLIGATGATYKTFIRNLRTKLTVGTPRVYDIPVLRNAA 71

Query: 77  KGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYFLKDVSKEAHDVLFKGSKHE 136
            GLARF LV LTNY GES+TVA+D V+VY+VAY+AGN AYFL D S EA++VLF G  H 
Sbjct: 72  AGLARFQLVTLTNYNGESVTVALDVVNVYVVAYRAGNTAYFLADASTEANNVLFAGINHV 131

Query: 137 TLSYKGNYDDLERHAGKISRENIELGFSELSSSIGNMFHYKAGYSVPRSFIVMIQMLSEA 196
            L Y GNYD LE  AG+ISRENIELGFSE+SS+IGNMF +  G SVPR+FIV+IQ +SEA
Sbjct: 132 RLPYGGNYDGLETAAGRISRENIELGFSEISSAIGNMFRHNPGTSVPRAFIVIIQTVSEA 191

Query: 197 SRFKYIETRNCEKVESKFKPDPGYLSLENRWSNLSEQVQKAQKHGGKFEHPVQVRSVSNK 256
           +RFKYIE R  E V +KFKPDP +LSL+N W +LSEQ+Q AQ  GG+F  PV++R+VSN 
Sbjct: 192 ARFKYIEQRVSENVGTKFKPDPAFLSLQNAWGSLSEQIQIAQTRGGEFARPVELRTVSNT 251

Query: 257 PVLVSDVNSPVVKGIALLL 276
           P  V++VNSPVVKGIALLL
Sbjct: 252 PTFVTNVNSPVVKGIALLL 263

[Close]

BLAST of CmoCh01G010040 vs. NCBI nr
Match: gi|530776695|emb|CCD28533.1| (ribosome inactivating protein, partial [Momordica charantia])

HSP 1 Score: 216.1 bits (549), Expect = 8.6e-53
Identity = 121/250 (48.40%), Postives = 173/250 (69.20%), Query Frame = 1


Query: 30  PSANANLSFSLLGATAQTYKQFINDLRKALTVNSHKVYDIPVLPATAKGLARFILVNLTN 89
           P+A  +++F L  ATA+TY +FI D R  L   SHKVYDIP+L +T  G  RFIL+NLT+
Sbjct: 13  PTAKGDVNFDLSTATAKTYTKFIEDFRATLPF-SHKVYDIPLLYSTISGSRRFILLNLTS 72

Query: 90  YKGESITVAIDTVDVYIVAYQAGNKAYFLKDVSKEAHDVLFKGSKHETLSYKGNYDDLER 149
           Y  E+I+VAID  +VY+VAY+  + +YF K+   EA+++LFKG++  TL Y GNY++L+ 
Sbjct: 73  YAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKGTRKITLPYTGNYENLQT 132

Query: 150 HAGKISRENIELGFSELSSSIGNMFHYKAGYSVPRSFIVMIQMLSEASRFKYIETRNCEK 209
            A KI RENI+LG   LSS+I  +F+Y A  S P + +V+IQ  +EA+RFKYIE    + 
Sbjct: 133 AAHKI-RENIDLGLPALSSAITTLFYYNA-QSAPSALLVLIQTTAEAARFKYIERHVAKY 192

Query: 210 VESKFKPDPGYLSLENRWSNLSEQVQKAQKHGGKFEHPVQVRSVSNKPVLVSDVNSPVVK 269
           V + FKP+   +SLEN+WS LS+Q+  AQ  GGKF +PV +   + +   V++V+S VVK
Sbjct: 193 VATNFKPNLAIISLENQWSALSKQIFLAQNQGGKFRNPVDLIKPTGERFQVTNVDSDVVK 252

Query: 270 G-IALLLSNK 279
           G I LLL+++
Sbjct: 253 GNIKLLLNSR 259

[Close]

BLAST of CmoCh01G010040 vs. NCBI nr
Match: gi|530776699|emb|CCD28535.1| (ribosome inactivating protein, partial [Momordica charantia])

HSP 1 Score: 215.7 bits (548), Expect = 1.1e-52
Identity = 121/250 (48.40%), Postives = 173/250 (69.20%), Query Frame = 1


Query: 30  PSANANLSFSLLGATAQTYKQFINDLRKALTVNSHKVYDIPVLPATAKGLARFILVNLTN 89
           P+A  +++F L  ATA+TY +FI D R  L   SHKVYDIP+L +T     RFIL+NLT+
Sbjct: 13  PTAKGDVNFDLSTATAKTYTKFIEDFRATLPF-SHKVYDIPLLYSTISDSRRFILLNLTS 72

Query: 90  YKGESITVAIDTVDVYIVAYQAGNKAYFLKDVSKEAHDVLFKGSKHETLSYKGNYDDLER 149
           Y  E+I+VAID  +VY+VAY+  + +YF K+   EA+++LFKG++  TL Y GNY++L+ 
Sbjct: 73  YAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKGTRKITLPYTGNYENLQT 132

Query: 150 HAGKISRENIELGFSELSSSIGNMFHYKAGYSVPRSFIVMIQMLSEASRFKYIETRNCEK 209
            A KI RENI+LGF  LSS+I  +F+Y A  S P + +V+IQ  +EA+RFKYIE    + 
Sbjct: 133 AAHKI-RENIDLGFPALSSAITTLFYYNA-QSAPSALLVLIQTTAEAARFKYIERHVAKY 192

Query: 210 VESKFKPDPGYLSLENRWSNLSEQVQKAQKHGGKFEHPVQVRSVSNKPVLVSDVNSPVVK 269
           V + FKP+   +SLEN+WS LS+Q+  AQ  GGKF +PV +   + +   V++V+S VVK
Sbjct: 193 VATNFKPNLAIISLENQWSALSKQIFLAQNQGGKFRNPVDLIKPTGERFQVTNVDSDVVK 252

Query: 270 G-IALLLSNK 279
           G I LLL+++
Sbjct: 253 GNIKLLLNSR 259

[Close]

BLAST of CmoCh01G010040 vs. NCBI nr
Match: gi|109676414|gb|ABG37691.1| (anti-HIV protein [Momordica charantia])

HSP 1 Score: 213.4 bits (542), Expect = 5.6e-52
Identity = 120/250 (48.00%), Postives = 172/250 (68.80%), Query Frame = 1


Query: 30  PSANANLSFSLLGATAQTYKQFINDLRKALTVNSHKVYDIPVLPATAKGLARFILVNLTN 89
           P+A  +++F L  ATA+TY +FI D R  L   SHKVYDIP+L +T     RFIL+NLT+
Sbjct: 19  PTAKGDVNFDLSTATAKTYTKFIEDFRATLPF-SHKVYDIPLLYSTISDSRRFILLNLTS 78

Query: 90  YKGESITVAIDTVDVYIVAYQAGNKAYFLKDVSKEAHDVLFKGSKHETLSYKGNYDDLER 149
           Y  E+I+VAID  +VY+VAY+  + +YF K+   EA+++LFKG++  TL Y GNY++L+ 
Sbjct: 79  YAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKGTRKITLPYTGNYENLQT 138

Query: 150 HAGKISRENIELGFSELSSSIGNMFHYKAGYSVPRSFIVMIQMLSEASRFKYIETRNCEK 209
            A KI RENI+LG   LSS+I  +F+Y A  S P + +V+IQ  +EA+RFKYIE    + 
Sbjct: 139 AAHKI-RENIDLGLPALSSAITTLFYYNA-QSAPSALLVLIQTTAEAARFKYIERHVAKY 198

Query: 210 VESKFKPDPGYLSLENRWSNLSEQVQKAQKHGGKFEHPVQVRSVSNKPVLVSDVNSPVVK 269
           V + FKP+   +SLEN+WS LS+Q+  AQ  GGKF +PV +   + +   V++V+S VVK
Sbjct: 199 VATNFKPNLAIISLENQWSALSKQIFLAQNQGGKFRNPVDLIKPTGERFQVTNVDSDVVK 258

Query: 270 G-IALLLSNK 279
           G I LLL+++
Sbjct: 259 GNIKLLLNSR 265

The following BLAST results are available for this feature:

BLAST of CmoCh01G010040 vs. Swiss-Prot


Match Name	E-value	Identity	Description
RIP2_BRYDI	1.0e-82	62.16	Ribosome-inactivating protein bryodin II OS=Bryonia dioica PE=1 SV=1	[more]
RIP3_MOMCH	3.5e-54	48.00	Ribosome-inactivating protein beta-momorcharin OS=Momordica charantia GN=MAP30 P...	[more]
RIP2_MOMBA	1.3e-53	47.60	Ribosome-inactivating protein momordin II OS=Momordica balsamina PE=3 SV=1	[more]
RIP_CUCMO	4.0e-50	47.33	Ribosome-inactivating protein cucurmosin OS=Cucurbita moschata PE=1 SV=2	[more]
RIPT_TRIKI	9.2e-47	44.40	Ribosome-inactivating protein alpha-trichosanthin OS=Trichosanthes kirilowii PE=...	[more]

BLAST of CmoCh01G010040 vs. TrEMBL


Match Name	E-value	Identity	Description
B1B626_TRIKI	1.2e-82	64.52	rRNA N-glycosidase OS=Trichosanthes kirilowii var. japonica PE=2 SV=1	[more]
S6CNA0_MOMCH	6.0e-53	48.40	Ribosome inactivating protein (Fragment) OS=Momordica charantia GN=rip PE=4 SV=1	[more]
S6CNH6_MOMCH	7.8e-53	48.40	Ribosome inactivating protein (Fragment) OS=Momordica charantia GN=rip PE=4 SV=1	[more]
Q5PZ05_MOMCH	3.9e-52	48.00	rRNA N-glycosidase OS=Momordica charantia PE=3 SV=1	[more]
S6CMM2_MOMCH	3.9e-52	48.00	Ribosome inactivating protein (Fragment) OS=Momordica charantia GN=rip PE=4 SV=1	[more]

BLAST of CmoCh01G010040 vs. TAIR10


Match Name	E-value	Identity	Description

BLAST of CmoCh01G010040 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|169635959\|dbj\|BAG12408.1\|	1.8e-82	64.52	karasurin-H precursor [Trichosanthes kirilowii var. japonica]	[more]
gi\|12643707\|sp\|P98184.1\|RIP2_BRYDI	1.7e-80	62.16	RecName: Full=Ribosome-inactivating protein bryodin II; AltName: Full=BD2; AltNa...	[more]
gi\|530776695\|emb\|CCD28533.1\|	8.6e-53	48.40	ribosome inactivating protein, partial [Momordica charantia]	[more]
gi\|530776699\|emb\|CCD28535.1\|	1.1e-52	48.40	ribosome inactivating protein, partial [Momordica charantia]	[more]
gi\|109676414\|gb\|ABG37691.1\|	5.6e-52	48.00	anti-HIV protein [Momordica charantia]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR001574	Ribosome_inactivat_prot
IPR016138	Ribosome_inactivat_prot_sub1
IPR016139	Ribosome_inactivat_prot_sub2
IPR017989	Ribosome_inactivat_1/2

Vocabulary: **Biological Process**
Term	Definition
GO:0017148	negative regulation of translation

Vocabulary: **Molecular Function**
Term	Definition
GO:0030598	rRNA N-glycosylase activity

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006952	defense response
biological_process	GO:0017148	negative regulation of translation
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0030598	rRNA N-glycosylase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh01G010040.1	CmoCh01G010040.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata
Date Performed: 2017-05-19


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001574	Ribosome-inactivating protein	PFAM	PF00161	RIP	coord: 44..235 score: 1.7
IPR001574	Ribosome-inactivating protein	unknown	SSF56371	Ribosome inactivating proteins (RIP)	coord: 36..276 score: 3.8
IPR016138	Ribosome-inactivating protein, subdomain 1	GENE3D	G3DSA:3.40.420.10		coord: 35..196 score: 2.1
IPR016139	Ribosome-inactivating protein, subdomain 2	GENE3D	G3DSA:4.10.470.10		coord: 198..277 score: 8.7
IPR017989	Ribosome-inactivating protein subgroup	PRINTS	PR00396	SHIGARICIN	coord: 218..235 score: 1.9E-29coord: 94..109 score: 1.9E-29coord: 46..59 score: 1.9E-29coord: 156..170 score: 1.9E-29coord: 186..206 score: 1.9

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CmoCh01G010040	Carg27773	Silver-seed gourd	carcmoB0897

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CmoCh01G010040	Wild cucumber (PI 183967)	cmocpiB484
CmoCh01G010040	Cucumber (Chinese Long) v2	cmocuB478
CmoCh01G010040	Melon (DHL92) v3.5.1	cmomeB387
CmoCh01G010040	Watermelon (Charleston Gray)	cmowcgB379
CmoCh01G010040	Watermelon (97103) v1	cmowmB405
CmoCh01G010040	Cucurbita pepo (Zucchini)	cmocpeB448
CmoCh01G010040	Cucurbita pepo (Zucchini)	cmocpeB464
CmoCh01G010040	Bottle gourd (USVL1VR-Ls)	cmolsiB397
CmoCh01G010040	Cucumber (Gy14) v2	cgybcmoB914
CmoCh01G010040	Melon (DHL92) v3.6.1	cmomedB444
CmoCh01G010040	Silver-seed gourd	carcmoB0093
CmoCh01G010040	Silver-seed gourd	carcmoB1120
CmoCh01G010040	Cucumber (Chinese Long) v3	cmocucB0574
CmoCh01G010040	Watermelon (97103) v2	cmowmbB498
CmoCh01G010040	Wax gourd	cmowgoB0621
CmoCh01G010040	Cucurbita moschata (Rifu)	cmocmoB018
CmoCh01G010040	Cucurbita moschata (Rifu)	cmocmoB367
CmoCh01G010040	Cucumber (Gy14) v1	cgycmoB0135
CmoCh01G010040	Cucurbita maxima (Rimu)	cmacmoB048
CmoCh01G010040	Cucurbita maxima (Rimu)	cmacmoB044
CmoCh01G010040	Cucurbita maxima (Rimu)	cmacmoB468