Carg27773 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg27773
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	rRNA N-glycosidase
Location	Cucurbita_argyrosperma_scaffold_363 : 43233 .. 44150 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGTGCATTGGATCAACCCAAGACTTGATACAAGCTCCCTTTCCCTTAAACTGTGGTGCTCATGCTGCCATTCGTGCTCCTCGTCCTTCCGCCAATGCTAATCTCAGCTTCTCTCTGTTGGGTGCCACTGCCCAAACCTACAAACAATTCATTAATGATCTTCGTAAGGCACTTACGGTAAACTCCCACAAAGTGTACGATATTCCTGTGCTGCCAGCCACGGCCAAAGGGTTAGCACGCTTCATATTGGTCAATCTCACCAATTACAAAGGTGAATCCATCACTGTTGCAATCGACACAGTGGATGTCTATATTGTGGCATACCAAGCAGGAAACAAGGCTTATTTTCTAAAAGATGTTTCCAAAGAAGCCCATGATGTGCTATTTAAAGGTAGCAAACATGAAACGCTGTCGTATAAAGGGAATTATGATGATCTTGAGAGGCATGCAGGTAAGATTTCAAGAGAGAATATTGAGCTTGGATTCTCTGAATTAAGCAGCTCCATTGGCAACATGTTCCACTATAACGCTGGCTATTCTGTGCCTAGATCGTTTATTGTGATGATTCAAATGCTTTCAGAAGCTTCGAGGTTTAAGTATATTGAGACGAGAAATTGTGAGAAGGTTGAATCAAAGTTTAAGCCAGATCCTGGATACTTGAGTTTAGAGAACCGTTGGTCTAATTTATCTGAACAGGTACAGAAGGCGCAAAAGCATGGAGGTAAATTTGAGCATCCAGTTCAAGTTCGAAGTGTTAGCAATAAGCCAGTTCTGGTGAGTGATGTTAATTCCCCTGTTGTTAAAGGAATAGCGCTGTTACTTTGCAACAAGCCAGGGGTTAATGGAGTATCCAAATTGATGGAAGAGTTTATCAAATGGGAAGCTGCTCGAGGTGCTTTTGGAATTAATTTTTGA

mRNA sequence

ATGGAGTGCATTGGATCAACCCAAGACTTGATACAAGCTCCCTTTCCCTTAAACTGTGGTGCTCATGCTGCCATTCGTGCTCCTCGTCCTTCCGCCAATGCTAATCTCAGCTTCTCTCTGTTGGGTGCCACTGCCCAAACCTACAAACAATTCATTAATGATCTTCGTAAGGCACTTACGGTAAACTCCCACAAAGTGTACGATATTCCTGTGCTGCCAGCCACGGCCAAAGGGTTAGCACGCTTCATATTGGTCAATCTCACCAATTACAAAGGTGAATCCATCACTGTTGCAATCGACACAGTGGATGTCTATATTGTGGCATACCAAGCAGGAAACAAGGCTTATTTTCTAAAAGATGTTTCCAAAGAAGCCCATGATGTGCTATTTAAAGGTAGCAAACATGAAACGCTGTCGTATAAAGGGAATTATGATGATCTTGAGAGGCATGCAGGTAAGATTTCAAGAGAGAATATTGAGCTTGGATTCTCTGAATTAAGCAGCTCCATTGGCAACATGTTCCACTATAACGCTGGCTATTCTGTGCCTAGATCGTTTATTGTGATGATTCAAATGCTTTCAGAAGCTTCGAGGTTTAAGTATATTGAGACGAGAAATTGTGAGAAGGTTGAATCAAAGTTTAAGCCAGATCCTGGATACTTGAGTTTAGAGAACCGTTGGTCTAATTTATCTGAACAGGTACAGAAGGCGCAAAAGCATGGAGGTAAATTTGAGCATCCAGTTCAAGTTCGAAGTGTTAGCAATAAGCCAGTTCTGGTGAGTGATGTTAATTCCCCTGTTGTTAAAGGAATAGCGCTGTTACTTTGCAACAAGCCAGGGGTTAATGGAGTATCCAAATTGATGGAAGAGTTTATCAAATGGGAAGCTGCTCGAGGTGCTTTTGGAATTAATTTTTGA

Coding sequence (CDS)

ATGGAGTGCATTGGATCAACCCAAGACTTGATACAAGCTCCCTTTCCCTTAAACTGTGGTGCTCATGCTGCCATTCGTGCTCCTCGTCCTTCCGCCAATGCTAATCTCAGCTTCTCTCTGTTGGGTGCCACTGCCCAAACCTACAAACAATTCATTAATGATCTTCGTAAGGCACTTACGGTAAACTCCCACAAAGTGTACGATATTCCTGTGCTGCCAGCCACGGCCAAAGGGTTAGCACGCTTCATATTGGTCAATCTCACCAATTACAAAGGTGAATCCATCACTGTTGCAATCGACACAGTGGATGTCTATATTGTGGCATACCAAGCAGGAAACAAGGCTTATTTTCTAAAAGATGTTTCCAAAGAAGCCCATGATGTGCTATTTAAAGGTAGCAAACATGAAACGCTGTCGTATAAAGGGAATTATGATGATCTTGAGAGGCATGCAGGTAAGATTTCAAGAGAGAATATTGAGCTTGGATTCTCTGAATTAAGCAGCTCCATTGGCAACATGTTCCACTATAACGCTGGCTATTCTGTGCCTAGATCGTTTATTGTGATGATTCAAATGCTTTCAGAAGCTTCGAGGTTTAAGTATATTGAGACGAGAAATTGTGAGAAGGTTGAATCAAAGTTTAAGCCAGATCCTGGATACTTGAGTTTAGAGAACCGTTGGTCTAATTTATCTGAACAGGTACAGAAGGCGCAAAAGCATGGAGGTAAATTTGAGCATCCAGTTCAAGTTCGAAGTGTTAGCAATAAGCCAGTTCTGGTGAGTGATGTTAATTCCCCTGTTGTTAAAGGAATAGCGCTGTTACTTTGCAACAAGCCAGGGGTTAATGGAGTATCCAAATTGATGGAAGAGTTTATCAAATGGGAAGCTGCTCGAGGTGCTTTTGGAATTAATTTTTGA

Protein sequence

MECIGSTQDLIQAPFPLNCGAHAAIRAPRPSANANLSFSLLGATAQTYKQFINDLRKALTVNSHKVYDIPVLPATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYFLKDVSKEAHDVLFKGSKHETLSYKGNYDDLERHAGKISRENIELGFSELSSSIGNMFHYNAGYSVPRSFIVMIQMLSEASRFKYIETRNCEKVESKFKPDPGYLSLENRWSNLSEQVQKAQKHGGKFEHPVQVRSVSNKPVLVSDVNSPVVKGIALLLCNKPGVNGVSKLMEEFIKWEAARGAFGINF

Homology

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BLAST of Carg27773 vs. NCBI nr
Match: XP_022926255.1 (ribosome-inactivating protein bryodin II-like [Cucurbita moschata])

HSP 1 Score: 595.9 bits (1535), Expect = 7.8e-167
Identity = 300/305 (98.36%), Postives = 301/305 (98.69%), Query Frame = 0


Query: 1   MECIGSTQDLIQAPFPLNCGAHAAIRAPRPSANANLSFSLLGATAQTYKQFINDLRKALT 60
           MECIGSTQDLIQAPFPLNCGAHAAIRAPRPSANANLSFSLLGATAQTYKQFINDLRKALT
Sbjct: 1   MECIGSTQDLIQAPFPLNCGAHAAIRAPRPSANANLSFSLLGATAQTYKQFINDLRKALT 60

Query: 61  VNSHKVYDIPVLPATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYFLKD 120
           VNSHKVYDIPVLPATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYFLKD
Sbjct: 61  VNSHKVYDIPVLPATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYFLKD 120

Query: 121 VSKEAHDVLFKGSKHETLSYKGNYDDLERHAGKISRENIELGFSELSSSIGNMFHYNAGY 180
           VSKEAHDVLFKGSKHETLSYKGNYDDLERHAGKISRENIELGFSELSSSIGNMFHY AGY
Sbjct: 121 VSKEAHDVLFKGSKHETLSYKGNYDDLERHAGKISRENIELGFSELSSSIGNMFHYKAGY 180

Query: 181 SVPRSFIVMIQMLSEASRFKYIETRNCEKVESKFKPDPGYLSLENRWSNLSEQVQKAQKH 240
           SVPRSFIVMIQMLSEASRFKYIETRNCEKVESKFKPDPGYLSLENRWSNLSEQVQKAQKH
Sbjct: 181 SVPRSFIVMIQMLSEASRFKYIETRNCEKVESKFKPDPGYLSLENRWSNLSEQVQKAQKH 240

Query: 241 GGKFEHPVQVRSVSNKPVLVSDVNSPVVKGIALLLCNKPGVNGVSKLMEEFIKWEAARGA 300
           GGKFEHPVQVRSVSNKPVLVSDVNSPVVKGIALLL NKPGVNGVSKLMEEFI WEAARG 
Sbjct: 241 GGKFEHPVQVRSVSNKPVLVSDVNSPVVKGIALLLSNKPGVNGVSKLMEEFIHWEAARGH 300

Query: 301 FGINF 306
           FGI+F
Sbjct: 301 FGIDF 305

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BLAST of Carg27773 vs. NCBI nr
Match: XP_022981562.1 (ribosome-inactivating protein bryodin II-like [Cucurbita maxima])

HSP 1 Score: 592.0 bits (1525), Expect = 1.1e-165
Identity = 295/305 (96.72%), Postives = 301/305 (98.69%), Query Frame = 0


Query: 1   MECIGSTQDLIQAPFPLNCGAHAAIRAPRPSANANLSFSLLGATAQTYKQFINDLRKALT 60
           MECIGST+DLIQAPFPLNCGAH AIRAPRPSANANLSFSLLGATAQTYKQFI+DLRKALT
Sbjct: 1   MECIGSTKDLIQAPFPLNCGAHVAIRAPRPSANANLSFSLLGATAQTYKQFISDLRKALT 60

Query: 61  VNSHKVYDIPVLPATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYFLKD 120
           VNSHKVYDIPVLPATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYFLKD
Sbjct: 61  VNSHKVYDIPVLPATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYFLKD 120

Query: 121 VSKEAHDVLFKGSKHETLSYKGNYDDLERHAGKISRENIELGFSELSSSIGNMFHYNAGY 180
           VSKEAHDVLFKGSKHETLSYKGNYDDLERHAGKISRENI+LGFSELSSSIGNMFHYNAGY
Sbjct: 121 VSKEAHDVLFKGSKHETLSYKGNYDDLERHAGKISRENIDLGFSELSSSIGNMFHYNAGY 180

Query: 181 SVPRSFIVMIQMLSEASRFKYIETRNCEKVESKFKPDPGYLSLENRWSNLSEQVQKAQKH 240
           SVP+SFIVMIQMLSEASRFKYIE RNCE VESKFKPDPGYLSLENRWSNLSEQVQKAQKH
Sbjct: 181 SVPKSFIVMIQMLSEASRFKYIEARNCENVESKFKPDPGYLSLENRWSNLSEQVQKAQKH 240

Query: 241 GGKFEHPVQVRSVSNKPVLVSDVNSPVVKGIALLLCNKPGVNGVSKLMEEFIKWEAARGA 300
           GGKFEHPVQVRSVSNKPVLVSDVNSPVVKG+ALLLCNKPGVNGVSKLMEEFIKWEAARG 
Sbjct: 241 GGKFEHPVQVRSVSNKPVLVSDVNSPVVKGMALLLCNKPGVNGVSKLMEEFIKWEAARGH 300

Query: 301 FGINF 306
           FGI+F
Sbjct: 301 FGIDF 305

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BLAST of Carg27773 vs. NCBI nr
Match: XP_023523582.1 (ribosome-inactivating protein bryodin II-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 587.0 bits (1512), Expect = 3.6e-164
Identity = 294/305 (96.39%), Postives = 298/305 (97.70%), Query Frame = 0


Query: 1   MECIGSTQDLIQAPFPLNCGAHAAIRAPRPSANANLSFSLLGATAQTYKQFINDLRKALT 60
           MECIGSTQDLIQAPFPLNCGAHAAIRAPRPSANANLSFSLLGATAQTYKQFINDLR ALT
Sbjct: 1   MECIGSTQDLIQAPFPLNCGAHAAIRAPRPSANANLSFSLLGATAQTYKQFINDLRNALT 60

Query: 61  VNSHKVYDIPVLPATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYFLKD 120
           V SHKVY IPVLPATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYFLKD
Sbjct: 61  VQSHKVYGIPVLPATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYFLKD 120

Query: 121 VSKEAHDVLFKGSKHETLSYKGNYDDLERHAGKISRENIELGFSELSSSIGNMFHYNAGY 180
           VSKEAHDVLFKGSKHETLSYKGNYDDLERHAG ISRENIELGFSELSSSIGNMFHYNAGY
Sbjct: 121 VSKEAHDVLFKGSKHETLSYKGNYDDLERHAGMISRENIELGFSELSSSIGNMFHYNAGY 180

Query: 181 SVPRSFIVMIQMLSEASRFKYIETRNCEKVESKFKPDPGYLSLENRWSNLSEQVQKAQKH 240
           SVPRSFIVMIQMLSEASRFKYIETRNCEKVESKFKPDPGYLSLENRWSNLSEQVQKAQKH
Sbjct: 181 SVPRSFIVMIQMLSEASRFKYIETRNCEKVESKFKPDPGYLSLENRWSNLSEQVQKAQKH 240

Query: 241 GGKFEHPVQVRSVSNKPVLVSDVNSPVVKGIALLLCNKPGVNGVSKLMEEFIKWEAARGA 300
           GGKFE PVQVRSVSNKP+ V+DVNSPVVKGIALLLCNKPGVNGVSKLME+FI WEAARGA
Sbjct: 241 GGKFERPVQVRSVSNKPIKVTDVNSPVVKGIALLLCNKPGVNGVSKLMEQFIHWEAARGA 300

Query: 301 FGINF 306
           FGI+F
Sbjct: 301 FGIDF 305

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BLAST of Carg27773 vs. NCBI nr
Match: XP_022158191.1 (ribosome-inactivating protein bryodin II-like [Momordica charantia])

HSP 1 Score: 390.2 bits (1001), Expect = 6.5e-105
Identity = 211/313 (67.41%), Postives = 249/313 (79.55%), Query Frame = 0


Query: 1   MECIGSTQDLIQAPFPLN----CGAHA--AIRAPRPSANANLSFSLLGATAQTYKQFIND 60
           MEC+ S++  IQ P P N     G HA  A+ AP    N+N+SFSLLGAT +TY+QFI +
Sbjct: 1   MECVRSSEPFIQ-PLPFNDIGIHGVHANGAVNAP----NSNVSFSLLGATGKTYQQFIQN 60

Query: 61  LRKALTVNSHKVYDIPVLPATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNK 120
           LR ALT+NS  VY+IPVL ATA   ARFILV+LTNYK E+ITVAID V+VYIVAYQAGNK
Sbjct: 61  LRNALTINSKIVYNIPVLAATALSSARFILVHLTNYKNETITVAIDVVNVYIVAYQAGNK 120

Query: 121 AYFLKDVSKEAHDVLFKGSKHETLSYKGNYDDLERHAGKISRENIELGFSELSSSIGNMF 180
           AYFLKD SKEA DVLFKG K E L YKGNYD LE  AGKISRE I+LGFSEL S+IGNM+
Sbjct: 121 AYFLKDASKEARDVLFKGIKQEILPYKGNYDGLETAAGKISREKIDLGFSELGSAIGNMY 180

Query: 181 HYNAGYSVPRSFIVMIQMLSEASRFKYIETRNCEKVESKFKPDPGYLSLENRWSNLSEQV 240
           HYNAG SVPR+FIVMIQ +SEA+RFKYIET+  + VE+KFKPDPG+LSLENRWS+LSEQV
Sbjct: 181 HYNAGTSVPRAFIVMIQTISEAARFKYIETKVSQNVETKFKPDPGFLSLENRWSDLSEQV 240

Query: 241 QKAQKHGGKFEHPVQVRSVSNKPVLVSDVNSPVVKGIALLLCNKPGVNG-VSKLMEEFIK 300
           Q AQ   G+F  P++VRSV+NKP+LV++V S VV+G+ALLL +KP  NG + +LMEEF K
Sbjct: 241 QIAQNRKGEFARPIEVRSVTNKPILVTNVKSKVVEGMALLLYSKPRGNGDLMELMEEFNK 300

Query: 301 -WEAARGAFGINF 306
            WE  RG FGI+F
Sbjct: 301 EWENGRGEFGIDF 308

[Close]

BLAST of Carg27773 vs. NCBI nr
Match: XP_022158193.1 (ribosome-inactivating protein bryodin II-like [Momordica charantia])

HSP 1 Score: 329.3 bits (843), Expect = 1.4e-86
Identity = 171/251 (68.13%), Postives = 206/251 (82.07%), Query Frame = 0


Query: 31  SANANLSFSLLGATAQTYKQFINDLRKALTVNSHKVYDIPVLPATAKGLARFILVNLTNY 90
           +A A++SFS+LG+T++TYKQFI ++R ALTV S  VYDIPVL ATA G ARF+LV+LTNY
Sbjct: 18  AAEADVSFSMLGSTSETYKQFIKNVRSALTVGSQIVYDIPVLKATATGSARFLLVHLTNY 77

Query: 91  KGESITVAIDTVDVYIVAYQAGNKAYFLKDVSKEAHDVLFKGSKHETLSYKGNYDDLERH 150
             ESI VAID V+VY+VAY+AGN AYFL D S EA  VLF+G  H  LSYKGNYD LE  
Sbjct: 78  NSESIIVAIDVVNVYVVAYRAGNNAYFLNDASAEADRVLFQGINHVKLSYKGNYDGLET- 137

Query: 151 AGKISRENIELGFSELSSSIGNMFHYNAGYSVPRSFIVMIQMLSEASRFKYIETRNCEKV 210
           A KISRE I+LGFSE+SSSIGNMFH+NAG SVPR+FIV+IQ +SEA+RFKYIE R  E V
Sbjct: 138 AAKISREKIDLGFSEISSSIGNMFHHNAGTSVPRAFIVIIQSVSEAARFKYIEQRVSENV 197

Query: 211 ESKFKPDPGYLSLENRWSNLSEQVQKAQKHGGKFEHPVQVRSVSNKPVLVSDVNSPVVKG 270
           ++ FKPDP +LSL+NRWS+LSEQ+Q AQK GG+F   V++R+VSNKP+LV+DV+SPVVKG
Sbjct: 198 KTTFKPDPAFLSLQNRWSDLSEQIQIAQKRGGEFARAVELRTVSNKPILVTDVSSPVVKG 257

Query: 271 IALLLCNKPGV 282
           IALLL  K  V
Sbjct: 258 IALLLYYKVSV 267

[Close]

BLAST of Carg27773 vs. Swiss-Prot
Match: sp|P98184|RIP2_BRYDI (Ribosome-inactivating protein bryodin II OS=Bryonia dioica OX=3652 PE=1 SV=1)

HSP 1 Score: 310.5 bits (794), Expect = 2.1e-83
Identity = 162/259 (62.55%), Postives = 197/259 (76.06%), Query Frame = 0


Query: 17  LNCGAHAAIRAPRPSANANLSFSLLGATAQTYKQFINDLRKALTVNSHKVYDIPVLPATA 76
           L  GAH        + + +++FSL+GAT  TYK FI +LR  LTV + +VYDIPVL   A
Sbjct: 12  LYVGAHV-------TEDVDINFSLIGATGATYKTFIRNLRTKLTVGTPRVYDIPVLRNAA 71

Query: 77  KGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYFLKDVSKEAHDVLFKGSKHE 136
            GLARF LV LTNY GES+TVA+D V+VY+VAY+AGN AYFL D S EA++VLF G  H 
Sbjct: 72  AGLARFQLVTLTNYNGESVTVALDVVNVYVVAYRAGNTAYFLADASTEANNVLFAGINHV 131

Query: 137 TLSYKGNYDDLERHAGKISRENIELGFSELSSSIGNMFHYNAGYSVPRSFIVMIQMLSEA 196
            L Y GNYD LE  AG+ISRENIELGFSE+SS+IGNMF +N G SVPR+FIV+IQ +SEA
Sbjct: 132 RLPYGGNYDGLETAAGRISRENIELGFSEISSAIGNMFRHNPGTSVPRAFIVIIQTVSEA 191

Query: 197 SRFKYIETRNCEKVESKFKPDPGYLSLENRWSNLSEQVQKAQKHGGKFEHPVQVRSVSNK 256
           +RFKYIE R  E V +KFKPDP +LSL+N W +LSEQ+Q AQ  GG+F  PV++R+VSN 
Sbjct: 192 ARFKYIEQRVSENVGTKFKPDPAFLSLQNAWGSLSEQIQIAQTRGGEFARPVELRTVSNT 251

Query: 257 PVLVSDVNSPVVKGIALLL 276
           P  V++VNSPVVKGIALLL
Sbjct: 252 PTFVTNVNSPVVKGIALLL 263

[Close]

BLAST of Carg27773 vs. Swiss-Prot
Match: sp|P24817|RIP3_MOMCH (Ribosome-inactivating protein beta-momorcharin OS=Momordica charantia OX=3673 GN=MAP30 PE=1 SV=2)

HSP 1 Score: 215.7 bits (548), Expect = 7.2e-55
Identity = 120/248 (48.39%), Postives = 169/248 (68.15%), Query Frame = 0


Query: 30  PSANANLSFSLLGATAQTYKQFINDLRKALTVNSHKVYDIPVLPATAKGLARFILVNLTN 89
           P+A  +++F L  ATA+TY +FI D R  L   SHKVYDIP+L +T     RFIL+NLT+
Sbjct: 19  PTAKGDVNFDLSTATAKTYTKFIEDFRATLPF-SHKVYDIPLLYSTISDSRRFILLNLTS 78

Query: 90  YKGESITVAIDTVDVYIVAYQAGNKAYFLKDVSKEAHDVLFKGSKHETLSYKGNYDDLER 149
           Y  E+I+VAID  +VY+VAY+  + +YF K+   EA+++LFKG++  TL Y GNY++L+ 
Sbjct: 79  YAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKGTRKITLPYTGNYENLQT 138

Query: 150 HAGKISRENIELGFSELSSSIGNMFHYNAGYSVPRSFIVMIQMLSEASRFKYIETRNCEK 209
            A KI RENI+LG   LSS+I  +F+YNA  S P + +V+IQ  +EA+RFKYIE    + 
Sbjct: 139 AAHKI-RENIDLGLPALSSAITTLFYYNA-QSAPSALLVLIQTTAEAARFKYIERHVAKY 198

Query: 210 VESKFKPDPGYLSLENRWSNLSEQVQKAQKHGGKFEHPVQVRSVSNKPVLVSDVNSPVVK 269
           V + FKP+   +SLEN+WS LS+Q+  AQ  GGKF +PV +   + +   V++V+S VVK
Sbjct: 199 VATNFKPNLAIISLENQWSALSKQIFLAQNQGGKFRNPVDLIKPTGERFQVTNVDSDVVK 258

Query: 270 GIALLLCN 278
           G   LL N
Sbjct: 259 GNIKLLLN 263

[Close]

BLAST of Carg27773 vs. Swiss-Prot
Match: sp|P29339|RIP2_MOMBA (Ribosome-inactivating protein momordin II OS=Momordica balsamina OX=3672 PE=3 SV=1)

HSP 1 Score: 213.8 bits (543), Expect = 2.7e-54
Identity = 119/248 (47.98%), Postives = 169/248 (68.15%), Query Frame = 0


Query: 30  PSANANLSFSLLGATAQTYKQFINDLRKALTVNSHKVYDIPVLPATAKGLARFILVNLTN 89
           P+A  +++F L  ATA+TY +FI D R  L   SHKVYDIP+L +T     RFIL++LT+
Sbjct: 19  PTAKGDVNFDLSTATAKTYTKFIEDFRATLPF-SHKVYDIPLLYSTISDSRRFILLDLTS 78

Query: 90  YKGESITVAIDTVDVYIVAYQAGNKAYFLKDVSKEAHDVLFKGSKHETLSYKGNYDDLER 149
           Y  E+I+VAID  +VY+VAY+  + +YF K+   EA+++LFKG++  TL Y GNY++L+ 
Sbjct: 79  YAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKGTRKITLPYTGNYENLQT 138

Query: 150 HAGKISRENIELGFSELSSSIGNMFHYNAGYSVPRSFIVMIQMLSEASRFKYIETRNCEK 209
            A KI RENI+LG   LSS+I  +F+YNA  S P + +V+IQ  +EA+RFKYIE    + 
Sbjct: 139 AAHKI-RENIDLGLPALSSAITTLFYYNA-QSAPSALLVLIQTTAEAARFKYIERHVAKY 198

Query: 210 VESKFKPDPGYLSLENRWSNLSEQVQKAQKHGGKFEHPVQVRSVSNKPVLVSDVNSPVVK 269
           V + FKP+   +SLEN+WS LS+Q+  AQ  GGKF +PV +   + +   V++V+S VVK
Sbjct: 199 VATNFKPNLAIISLENQWSALSKQIFLAQNQGGKFRNPVDLIKPTGERFQVTNVDSDVVK 258

Query: 270 GIALLLCN 278
           G   LL N
Sbjct: 259 GNIKLLLN 263

[Close]

BLAST of Carg27773 vs. Swiss-Prot
Match: sp|P84531|RIP_CUCMO (Ribosome-inactivating protein cucurmosin OS=Cucurbita moschata OX=3662 PE=1 SV=2)

HSP 1 Score: 201.8 bits (512), Expect = 1.1e-50
Identity = 116/242 (47.93%), Postives = 158/242 (65.29%), Query Frame = 0


Query: 35  NLSFSLLGATAQTYKQFINDLRKALTVNSHKVYDIPVLPATAKGLARFILVNLTNYKGES 94
           N+ F L  AT+ +YK FI +LR+AL  +  KVYDIPVL +T     RFIL++L NY G+S
Sbjct: 1   NVRFDLSSATSSSYKTFIKNLREALPKDG-KVYDIPVLLSTVMDSRRFILIDLVNYDGQS 60

Query: 95  ITVAIDTVDVYIVAYQAGNKAYFLKDVSKEAHDVLFKGSKHETLSYKGNYDDLERHAGKI 154
           IT AID ++VYIVAY  G  +YF + V  +A  +LFKG++  TL Y GNY++L+  A K+
Sbjct: 61  ITAAIDVLNVYIVAYSTGTVSYFFQQVPAQAPKLLFKGTQQRTLPYTGNYENLQTAAKKL 120

Query: 155 SRENIELGFSELSSSIGNMFHYNAGYSVPRSFIVMIQMLSEASRFKYIETRNCEKVESKF 214
            RENIELG   L S+I  +FHYNA  +   + +V+IQ  SEA+RF+YIE +    V +KF
Sbjct: 121 -RENIELGLPALDSAITTLFHYNA-EAAASALLVLIQTTSEAARFRYIELQIANNVGTKF 180

Query: 215 KPDPGYLSLENRWSNLSEQVQKAQKHGGKFEHPVQVRSVSNKPVLVSDVNSPVV-KGIAL 274
           KP    +SLEN WS LS+Q+Q A+   G+FE PV +       V +++V S VV + I L
Sbjct: 181 KPSQTIISLENNWSALSKQIQIAKNKNGQFETPVILIDPQGNRVQITNVTSNVVTQNIQL 239

Query: 275 LL 276
           LL
Sbjct: 241 LL 239

[Close]

BLAST of Carg27773 vs. Swiss-Prot
Match: sp|P09989|RIPT_TRIKI (Ribosome-inactivating protein alpha-trichosanthin OS=Trichosanthes kirilowii OX=3677 PE=1 SV=2)

HSP 1 Score: 191.0 bits (484), Expect = 1.9e-47
Identity = 110/251 (43.82%), Postives = 164/251 (65.34%), Query Frame = 0


Query: 30  PSANANLSFSLLGATAQTYKQFINDLRKALTVNSHKVYDIPVLPATAKGLARFILVNLTN 89
           P+   ++SF L GAT+ +Y  FI++LRKAL  N  K+YDIP+L ++  G  R+ L++LTN
Sbjct: 19  PAVEGDVSFRLSGATSSSYGVFISNLRKALP-NERKLYDIPLLRSSLPGSQRYALIHLTN 78

Query: 90  YKGESITVAIDTVDVYIVAYQAGNKAYFLKDVS-KEAHDVLFKGSKHE-TLSYKGNYDDL 149
           Y  E+I+VAID  +VYI+ Y+AG+ +YF  + S  EA   +FK +  + TL Y GNY+ L
Sbjct: 79  YADETISVAIDVTNVYIMGYRAGDTSYFFNEASATEAAKYVFKDAMRKVTLPYSGNYERL 138

Query: 150 ERHAGKISRENIELGFSELSSSIGNMFHYNAGYSVPRSFIVMIQMLSEASRFKYIETRNC 209
           +  AGKI RENI LG   L S+I  +F+YNA  S   + +V+IQ  SEA+R+K+IE +  
Sbjct: 139 QTAAGKI-RENIPLGLPALDSAITTLFYYNAN-SAASALMVLIQSTSEAARYKFIEQQIG 198

Query: 210 EKVESKFKPDPGYLSLENRWSNLSEQVQKAQKHGGKFEHPVQVRSVSNKPVLVSDVNSPV 269
           ++V+  F P    +SLEN WS LS+Q+Q A  + G+FE PV + +  N+ V +++V++ V
Sbjct: 199 KRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQFESPVVLINAQNQRVTITNVDAGV 258

Query: 270 VKGIALLLCNK 279
           V     LL N+
Sbjct: 259 VTSNIALLLNR 266

[Close]

BLAST of Carg27773 vs. TrEMBL
Match: tr|B1B626|B1B626_TRIKI (rRNA N-glycosidase OS=Trichosanthes kirilowii var. japonica OX=61011 PE=2 SV=1)

HSP 1 Score: 316.6 bits (810), Expect = 6.0e-83
Identity = 161/248 (64.92%), Postives = 198/248 (79.84%), Query Frame = 0


Query: 34  ANLSFSLLGATAQTYKQFINDLRKALTVNSHKVYDIPVLPATAKGLARFILVNLTNYKGE 93
           A+++FSLLG+T+ TYKQFI +LR ALTV S  V +IPVL  TA GLARF+LV+LTNY GE
Sbjct: 21  ADVNFSLLGSTSATYKQFIQNLRAALTVGSPTVENIPVLKGTAAGLARFLLVHLTNYNGE 80

Query: 94  SITVAIDTVDVYIVAYQAGNKAYFLKDVSKEAHDVLFKGSKHETLSYKGNYDDLERHAGK 153
           SITVA+D V+VY+VAY+AGN AYFL D S EA++VLFKG  H  L Y GNYD LE  AG+
Sbjct: 81  SITVAVDVVNVYVVAYRAGNNAYFLNDASAEANNVLFKGINHVRLPYGGNYDGLETAAGR 140

Query: 154 ISRENIELGFSELSSSIGNMFHYNAGYSVPRSFIVMIQMLSEASRFKYIETRNCEKVESK 213
           ISRE I LGFSE+SS+IG+MFHYNAG SVP++FIV+IQ +SEA+RFKYI+ R  E V++K
Sbjct: 141 ISREKIYLGFSEISSAIGSMFHYNAGTSVPKAFIVIIQTVSEAARFKYIQQRVSENVQTK 200

Query: 214 FKPDPGYLSLENRWSNLSEQVQKAQKHGGKFEHPVQVRSVSNKPVLVSDVNSPVVKGIAL 273
           F PDP +LSLEN W  LSEQ+Q AQ  GG+F  P+++R+VSN P  V++VNSPVVKG+AL
Sbjct: 201 FLPDPAFLSLENAWGGLSEQIQIAQYRGGEFARPIELRTVSNTPTFVTNVNSPVVKGVAL 260

Query: 274 LLCNKPGV 282
           LL  K  V
Sbjct: 261 LLYYKVNV 268

[Close]

BLAST of Carg27773 vs. TrEMBL
Match: tr|S6CNA0|S6CNA0_MOMCH (Ribosome inactivating protein (Fragment) OS=Momordica charantia OX=3673 GN=rip PE=4 SV=1)

HSP 1 Score: 218.4 bits (555), Expect = 2.2e-53
Identity = 121/248 (48.79%), Postives = 170/248 (68.55%), Query Frame = 0


Query: 30  PSANANLSFSLLGATAQTYKQFINDLRKALTVNSHKVYDIPVLPATAKGLARFILVNLTN 89
           P+A  +++F L  ATA+TY +FI D R  L   SHKVYDIP+L +T  G  RFIL+NLT+
Sbjct: 13  PTAKGDVNFDLSTATAKTYTKFIEDFRATLPF-SHKVYDIPLLYSTISGSRRFILLNLTS 72

Query: 90  YKGESITVAIDTVDVYIVAYQAGNKAYFLKDVSKEAHDVLFKGSKHETLSYKGNYDDLER 149
           Y  E+I+VAID  +VY+VAY+  + +YF K+   EA+++LFKG++  TL Y GNY++L+ 
Sbjct: 73  YAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKGTRKITLPYTGNYENLQT 132

Query: 150 HAGKISRENIELGFSELSSSIGNMFHYNAGYSVPRSFIVMIQMLSEASRFKYIETRNCEK 209
            A KI RENI+LG   LSS+I  +F+YNA  S P + +V+IQ  +EA+RFKYIE    + 
Sbjct: 133 AAHKI-RENIDLGLPALSSAITTLFYYNA-QSAPSALLVLIQTTAEAARFKYIERHVAKY 192

Query: 210 VESKFKPDPGYLSLENRWSNLSEQVQKAQKHGGKFEHPVQVRSVSNKPVLVSDVNSPVVK 269
           V + FKP+   +SLEN+WS LS+Q+  AQ  GGKF +PV +   + +   V++V+S VVK
Sbjct: 193 VATNFKPNLAIISLENQWSALSKQIFLAQNQGGKFRNPVDLIKPTGERFQVTNVDSDVVK 252

Query: 270 GIALLLCN 278
           G   LL N
Sbjct: 253 GNIKLLLN 257

[Close]

BLAST of Carg27773 vs. TrEMBL
Match: tr|S6CNH6|S6CNH6_MOMCH (Ribosome inactivating protein (Fragment) OS=Momordica charantia OX=3673 GN=rip PE=4 SV=1)

HSP 1 Score: 218.0 bits (554), Expect = 2.9e-53
Identity = 121/248 (48.79%), Postives = 170/248 (68.55%), Query Frame = 0


Query: 30  PSANANLSFSLLGATAQTYKQFINDLRKALTVNSHKVYDIPVLPATAKGLARFILVNLTN 89
           P+A  +++F L  ATA+TY +FI D R  L   SHKVYDIP+L +T     RFIL+NLT+
Sbjct: 13  PTAKGDVNFDLSTATAKTYTKFIEDFRATLPF-SHKVYDIPLLYSTISDSRRFILLNLTS 72

Query: 90  YKGESITVAIDTVDVYIVAYQAGNKAYFLKDVSKEAHDVLFKGSKHETLSYKGNYDDLER 149
           Y  E+I+VAID  +VY+VAY+  + +YF K+   EA+++LFKG++  TL Y GNY++L+ 
Sbjct: 73  YAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKGTRKITLPYTGNYENLQT 132

Query: 150 HAGKISRENIELGFSELSSSIGNMFHYNAGYSVPRSFIVMIQMLSEASRFKYIETRNCEK 209
            A KI RENI+LGF  LSS+I  +F+YNA  S P + +V+IQ  +EA+RFKYIE    + 
Sbjct: 133 AAHKI-RENIDLGFPALSSAITTLFYYNA-QSAPSALLVLIQTTAEAARFKYIERHVAKY 192

Query: 210 VESKFKPDPGYLSLENRWSNLSEQVQKAQKHGGKFEHPVQVRSVSNKPVLVSDVNSPVVK 269
           V + FKP+   +SLEN+WS LS+Q+  AQ  GGKF +PV +   + +   V++V+S VVK
Sbjct: 193 VATNFKPNLAIISLENQWSALSKQIFLAQNQGGKFRNPVDLIKPTGERFQVTNVDSDVVK 252

Query: 270 GIALLLCN 278
           G   LL N
Sbjct: 253 GNIKLLLN 257

[Close]

BLAST of Carg27773 vs. TrEMBL
Match: tr|S6CNY1|S6CNY1_MOMCH (Ribosome inactivating protein (Fragment) OS=Momordica charantia OX=3673 GN=rip PE=4 SV=1)

HSP 1 Score: 215.7 bits (548), Expect = 1.4e-52
Identity = 120/248 (48.39%), Postives = 169/248 (68.15%), Query Frame = 0


Query: 30  PSANANLSFSLLGATAQTYKQFINDLRKALTVNSHKVYDIPVLPATAKGLARFILVNLTN 89
           P+A  +++F L  ATA+TY +FI D R  L   SHKVYDIP+L +T     RFIL+NLT+
Sbjct: 13  PTAKGDVNFDLSTATAKTYTKFIEDFRATLPF-SHKVYDIPLLYSTISDSRRFILLNLTS 72

Query: 90  YKGESITVAIDTVDVYIVAYQAGNKAYFLKDVSKEAHDVLFKGSKHETLSYKGNYDDLER 149
           Y  E+I+VAID  +VY+VAY+  + +YF K+   EA+++LFKG++  TL Y GNY++L+ 
Sbjct: 73  YAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKGTRKITLPYTGNYENLQT 132

Query: 150 HAGKISRENIELGFSELSSSIGNMFHYNAGYSVPRSFIVMIQMLSEASRFKYIETRNCEK 209
            A KI RENI+LG   LSS+I  +F+YNA  S P + +V+IQ  +EA+RFKYIE    + 
Sbjct: 133 AAHKI-RENIDLGLPALSSAITTLFYYNA-QSAPSALLVLIQTTAEAARFKYIERHVAKY 192

Query: 210 VESKFKPDPGYLSLENRWSNLSEQVQKAQKHGGKFEHPVQVRSVSNKPVLVSDVNSPVVK 269
           V + FKP+   +SLEN+WS LS+Q+  AQ  GGKF +PV +   + +   V++V+S VVK
Sbjct: 193 VATNFKPNLAIISLENQWSALSKQIFLAQNQGGKFRNPVDLIKPTGERFQVTNVDSDVVK 252

Query: 270 GIALLLCN 278
           G   LL N
Sbjct: 253 GNIKLLLN 257

[Close]

BLAST of Carg27773 vs. TrEMBL
Match: tr|Q0ZBB9|Q0ZBB9_MOMCH (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=MAP30 PE=3 SV=1)

HSP 1 Score: 215.7 bits (548), Expect = 1.4e-52
Identity = 120/248 (48.39%), Postives = 169/248 (68.15%), Query Frame = 0


Query: 30  PSANANLSFSLLGATAQTYKQFINDLRKALTVNSHKVYDIPVLPATAKGLARFILVNLTN 89
           P+A  +++F L  ATA+TY +FI D R  L   SHKVYDIP+L +T     RFIL+NLT+
Sbjct: 19  PTAKGDVNFDLSTATAKTYTKFIEDFRATLPF-SHKVYDIPLLYSTISDSRRFILLNLTS 78

Query: 90  YKGESITVAIDTVDVYIVAYQAGNKAYFLKDVSKEAHDVLFKGSKHETLSYKGNYDDLER 149
           Y  E+I+VAID  +VY+VAY+  + +YF K+   EA+++LFKG++  TL Y GNY++L+ 
Sbjct: 79  YAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKGTRKITLPYTGNYENLQT 138

Query: 150 HAGKISRENIELGFSELSSSIGNMFHYNAGYSVPRSFIVMIQMLSEASRFKYIETRNCEK 209
            A KI RENI+LG   LSS+I  +F+YNA  S P + +V+IQ  +EA+RFKYIE    + 
Sbjct: 139 AAHKI-RENIDLGLPALSSAITTLFYYNA-QSAPSALLVLIQTTAEAARFKYIERHVAKY 198

Query: 210 VESKFKPDPGYLSLENRWSNLSEQVQKAQKHGGKFEHPVQVRSVSNKPVLVSDVNSPVVK 269
           V + FKP+   +SLEN+WS LS+Q+  AQ  GGKF +PV +   + +   V++V+S VVK
Sbjct: 199 VATNFKPNLAIISLENQWSALSKQIFLAQNQGGKFRNPVDLIKPTGERFQVTNVDSDVVK 258

Query: 270 GIALLLCN 278
           G   LL N
Sbjct: 259 GNIKLLLN 263

The following BLAST results are available for this feature:

BLAST of Carg27773 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022926255.1	7.8e-167	98.36	ribosome-inactivating protein bryodin II-like [Cucurbita moschata]	[more]
XP_022981562.1	1.1e-165	96.72	ribosome-inactivating protein bryodin II-like [Cucurbita maxima]	[more]
XP_023523582.1	3.6e-164	96.39	ribosome-inactivating protein bryodin II-like [Cucurbita pepo subsp. pepo]	[more]
XP_022158191.1	6.5e-105	67.41	ribosome-inactivating protein bryodin II-like [Momordica charantia]	[more]
XP_022158193.1	1.4e-86	68.13	ribosome-inactivating protein bryodin II-like [Momordica charantia]	[more]

BLAST of Carg27773 vs. TAIR10


Match Name	E-value	Identity	Description

BLAST of Carg27773 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|P98184\|RIP2_BRYDI	2.1e-83	62.55	Ribosome-inactivating protein bryodin II OS=Bryonia dioica OX=3652 PE=1 SV=1	[more]
sp\|P24817\|RIP3_MOMCH	7.2e-55	48.39	Ribosome-inactivating protein beta-momorcharin OS=Momordica charantia OX=3673 GN...	[more]
sp\|P29339\|RIP2_MOMBA	2.7e-54	47.98	Ribosome-inactivating protein momordin II OS=Momordica balsamina OX=3672 PE=3 SV...	[more]
sp\|P84531\|RIP_CUCMO	1.1e-50	47.93	Ribosome-inactivating protein cucurmosin OS=Cucurbita moschata OX=3662 PE=1 SV=2	[more]
sp\|P09989\|RIPT_TRIKI	1.9e-47	43.82	Ribosome-inactivating protein alpha-trichosanthin OS=Trichosanthes kirilowii OX=...	[more]

BLAST of Carg27773 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|B1B626\|B1B626_TRIKI	6.0e-83	64.92	rRNA N-glycosidase OS=Trichosanthes kirilowii var. japonica OX=61011 PE=2 SV=1	[more]
tr\|S6CNA0\|S6CNA0_MOMCH	2.2e-53	48.79	Ribosome inactivating protein (Fragment) OS=Momordica charantia OX=3673 GN=rip P...	[more]
tr\|S6CNH6\|S6CNH6_MOMCH	2.9e-53	48.79	Ribosome inactivating protein (Fragment) OS=Momordica charantia OX=3673 GN=rip P...	[more]
tr\|S6CNY1\|S6CNY1_MOMCH	1.4e-52	48.39	Ribosome inactivating protein (Fragment) OS=Momordica charantia OX=3673 GN=rip P...	[more]
tr\|Q0ZBB9\|Q0ZBB9_MOMCH	1.4e-52	48.39	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=MAP30 PE=3 SV=1	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Biological Process**
Term	Definition
GO:0017148	negative regulation of translation

Vocabulary: **Molecular Function**
Term	Definition
GO:0030598	rRNA N-glycosylase activity

Vocabulary: **INTERPRO**
Term	Definition
IPR036041	Ribosome-inact_prot_sf
IPR016139	Ribosome_inactivat_prot_sub2
IPR001574	Ribosome_inactivat_prot
IPR016138	Ribosome_inactivat_prot_sub1
IPR017989	Ribosome_inactivat_1/2

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0017148	negative regulation of translation
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0030598	rRNA N-glycosylase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg27773-RA	Carg27773-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR017989	Ribosome-inactivating protein type 1/2	PRINTS	PR00396	SHIGARICIN	coord: 218..235 score: 43.57 coord: 94..109 score: 44.9 coord: 46..59 score: 42.29 coord: 156..170 score: 48.77 coord: 186..206 score: 53.88
IPR016138	Ribosome-inactivating protein, subdomain 1	GENE3D	G3DSA:3.40.420.10		coord: 34..197 e-value: 1.3E-51 score: 176.5
IPR001574	Ribosome-inactivating protein	PFAM	PF00161	RIP	coord: 44..235 e-value: 1.7E-34 score: 119.1
IPR016139	Ribosome-inactivating protein, subdomain 2	GENE3D	G3DSA:4.10.470.10		coord: 198..280 e-value: 5.0E-24 score: 86.0
None	No IPR available	PANTHER	PTHR33453	FAMILY NOT NAMED	coord: 44..246
IPR036041	Ribosome-inactivating protein superfamily	SUPERFAMILY	SSF56371	Ribosome inactivating proteins (RIP)	coord: 36..276

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Carg27773	CmoCh01G010040	Cucurbita moschata (Rifu)	carcmoB0897

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Carg27773	Cucurbita maxima (Rimu)	carcmaB0923
Carg27773	Wild cucumber (PI 183967)	carcpiB0684
Carg27773	Cucumber (Chinese Long) v2	carcuB0674
Carg27773	Cucumber (Chinese Long) v3	carcucB0686
Carg27773	Bottle gourd (USVL1VR-Ls)	carlsiB594
Carg27773	Watermelon (97103) v1	carwmB0651
Carg27773	Watermelon (Charleston Gray)	carwcgB0634